> Chemicals & Drugs > Organic Chemical > Resazurin

Resazurin

Q: What are some common challenges in using Resazurin assays?

One challenge with Resazurin assays is ensuring reproducibility and accuracy. Factors like cell density, incubation time, and reagent concentrations can all impact the results. Additionally, there are different variations of Resazurin assays, such as the Alamar Blue assay, which can make it difficult to compare results across studies.

Resazurin is a blue, non-fluorescent dye that can be reduced to the pink, highly fluorescent resorufin by metabolically active cells.
This reduction process is commonly used in cell viability assays, as the amount of resorufin produced is proportional to the number of live cells.
Resazurin-based assays are widely used in drug discovery, toxicology, and cell biology research to evaluate cell proliferation, cytotoxicity, and metabolic activity.
The PubCompare.ai tool can help researchers optimized their Resazurin assay protocols by providing data-driven insights to identify the best procedures and products for their experiments, ensuring reproducibility and accuracy.
With its AI-driven capabilities, PubCompare.ai allows users to easily locate and compare Resazurin assay protocols from literature, pre-prints, and patents, leveraging this valuable information to improve the quality and efficiency of their research.

Most cited protocols related to «Resazurin»

Microdilution Assay for Antimicrobial Activity

Cited 204 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2007

Antibiotics Asepsis Bacteria Ciprofloxacin Complex Extracts Normal Saline Nutrients resazurin Sterility, Reproductive Strains Sulfoxide, Dimethyl Technique, Dilution

Antimicrobial Activity of Biosurfactants

Cited 81 times

Biosurfactants were dissolved in Muller Hinton broth (MHB) at twice the concentration of the final test, with pH adjusted to 7. 100 µl of the biosurfactant/MHB broth was dispensed in each well of Column 1, while Columns 2-10 contained 50 µl of MHB broth only. Column 11 contained 100 µl of diluted standardised inoculum, and Column 12 contained 100 µl of the medium broth (as a control to monitor sterility), as shown in processed plate Fig. 2. A multichannel pipette was then used to transfer and mix biosurfactants from column 1–10, resulting in 50 µl biosurfactant per well. The tested concentrations of the different biosursurfants achieved through double serial dilutions from columns 10–1 were as follows; 25–0.05 mg ml⁻¹ rhamnolipids (JBR325), 50–0.01 mg ml⁻¹ rhamnolipids from Burkholderia thailandensis E264, 12.5–0.025 mg ml⁻¹ of lactonic sophorolipids, 100–0.02 mg ml⁻¹ of acidic sophorolipids and 1–0.002 µg ml⁻¹ of polymyxin. The standardised microorganism suspension was then diluted by 1:100 in MHB broth. 50 µl of the adjusted OD₆₀₀ bacterial suspension was then added to all wells containing biosurfactant and to the control wells, resulting in approx. 5 x10⁵ CFU ml⁻¹. The time taken to prepare and dispense the OD adjusted bacteria did not exceed 15 min. After incubation for 24 h at 37 °C, resazurin (0.015 %) was added to all wells (30 µl per well), and further incubated for 2–4 h for the observation of colour change. On completion of the incubation, columns with no colour change (blue resazurin colour remained unchanged) were scored as above the MIC value. The minimum biocidal concentration (MBC) was determined by plating directly the content of wells with concentrations higher than the MIC value, as detailed in Table 1. The MBC value was determined when there was no colony growth from the directly plated contents of the wells. In addition the contents of the wells showing indications of growth inhibition were serially diluted to quantify an end-point killing of the bacteria as detailed in the results section.Fig. 2

Determination of MIC for Rhamnolipid JBR325 against Streptococcus mutans (DSM-20523). After the period of incubation, resazurin dye was added. Column 12 confirms no contamination occurred while preparing the plate. Column 11, a negative control shows a change of resazurin natural colour (blue/purple) to the reduced form (red-colourless). The highest concentration incorporated into the plate is 25 mg ml⁻¹ and the lowest achieved through double serial dilution is 0.05 mg ml⁻¹. Column 7 shows no colour changes therefore concentration of biosurfactant in that column was taken as the MIC value. The range of biosurfactant concentration in the wells was 25–0.05 mg ml⁻¹

Table 1

Determination of the MIC by Resazurin aided microdilution method of two antibiotics against two standard strains

Bacteria	Antibiotic	MIC reported in this study (µg ml⁻¹)	MIC recommended by CLSI (µg ml⁻¹)
Escherichia coli ATCC 25922	Tetracycline	2	0.5–2
Staphylococcus aureus ATCC 29213	Tetracycline	0.5	0.12–1
Escherichia coli ATCC 25922	Gentamicin	1	0.25–1
Staphylococcus aureus ATCC 29213	Gentamicin	0.5	0.12–1

Values obtained were compared with those recommended by the CLSI

Elshikh M., Ahmed S., Funston S., Dunlop P., McGaw M., Marchant R, & Banat I.M. (2016). Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology Letters, 38, 1015-1019.

Full text: Click here

Publication 2016

Validating Antibiotic Susceptibility Testing

Cited 39 times

To establish the accuracy of this method, it was first important to validate the performance of standard antibiotics against several ATCC strains to determine the MIC values and compare them with those published by The Clinical and Laboratory Standards Institute (M100-S22, Volume 32 No 3. January 2012). In this assay, two of the quality control ATCC strains, namely Gram-positive Staphylococcus aureus ATCC 29213 and Gram-negative Escherichia coli ATCC 25922, were screened against the antibiotics tetracycline (bacteriostatic in action) and gentamicin (bactericidal in action) and the MIC determined through recording of the colour change observed.

Full text: Click here

Publication 2016

Antibiotics, Antitubercular Biological Assay Clinical Laboratory Services Escherichia coli Gentamicin Staphylococcus aureus Strains Tetracycline

Optimizing Growth Media for Caldicellulosiruptor bescii

Cited 30 times

Modified DSMZ 640 medium [29 (link)], and a defined variant of this medium that lacks yeast extract and casein [7 (link)], has been described for the growth of C. bescii. For liquid media, a 1 % inoculum was used and cells were grown for 16–20 h at 75 °C, unless otherwise specified.
Initial liquid growth media contained sulfide and were prepared with mineral solution [8 (link)], trace element solution SL-10 [26 ], vitamin solution [28 (link)], 19 amino acid solution [1 (link)], 0.25 mg/l resazurin, 2.4 mM KH₂PO₄, and 5 g/l maltose. The media were prepared aerobically, 2 g/l NaHCO₃ was added, and the mixture was reduced using 3 g/l cysteine HCl and either 1 g/l Na₂S (as used previously), 0.5 g/l Na₂S or no Na₂S. The pH was adjusted to 6.8, and the media were aliquoted into serum bottles, sealed, and degassed by four cycles of vacuum and argon. The final pH after degassing was 7.2. Wild-type C. bescii was inoculated into 50-ml cultures and culture density was compared after 12 and 24 h of growth. Liquid growth media without sulfide were prepared with mineral solution [8 (link)], trace element solution SL-10 [26 ], vitamin solution [28 (link)], 0.25 mg/l resazurin, 2.4 mM KH₂PO₄, and 5 g/l maltose. In separate batches, the media were prepared with 19 amino acid solution [1 (link)], 0.05 % (w/v) casein hydrolysate, or both. The media were prepared aerobically, 2 g/l NaHCO₃ was added, and the mixture was reduced using 3 g/l cysteine HCl. The pH was adjusted to 6.8, and the media were aliquoted into serum bottles, sealed, and degassed by four cycles of vacuum and argon. The final pH after degassing was 7.2. Wild-type C. bescii was inoculated into 50-ml cultures and culture density was compared after 12 and 24 h of growth. Liquid growth media without sulfide, casein, or amino acids but varying the phosphate concentrations, were prepared with a modified mineral solution (containing 0.14 g/l CaCl₂ × 2H₂O but the same amounts of other salts), trace element solution SL-10 [26 ], vitamin solution [28 (link)], 0.25 mg/l resazurin, and 5 g/l maltose. These media were prepared with 2.4 mM, 1 mM, 100 μM, 10 μM, 1 μM, or no KH₂PO₄. The media were prepared aerobically, 2 g/l NaHCO₃ was added, and the mixture was reduced using 3 g/l cysteine HCl. The pH was adjusted to 6.8, and the media was aliquoted into serum bottles, sealed, and degassed by four cycles of vacuum and argon. The final pH after degassing was 7.2. Wild-type C. bescii was inoculated into 50-ml cultures and culture density was compared after 12 and 24 h of growth. Liquid growth media without sulfide, casein, or amino acids, with phosphate reduced to 10 μM were prepared with a modified mineral solution (containing 0.14 g/l CaCl₂ × 2H₂O but the same amounts of other salts), trace element solution SL-10 [26 ], vitamin solution [28 (link)], 0.25 mg/l resazurin, 100 μM KH₂PO₄, and 5 g/l maltose. The media were prepared aerobically, 2 g/l NaHCO₃ was added, and the mixture was reduced using 3 g/l cysteine HCl. In a separate batch, 1 g/l NaHCO₃ was added, and the mixture was reduced using 1 g/l cysteine HCl. The pH was adjusted to 6.8, and the media were aliquoted into serum bottles, sealed, and degassed by four cycles of vacuum and argon. The final pH after degassing was 7.2. Wild-type C. bescii was inoculated into 50-ml cultures and culture density was compared after 12 and 24 h of growth.
LOD and LOC media were prepared from filter sterilized stock solutions. The 50× CbeI partial base salt solution contains 16.5 g of MgCl₂, 16.5 g of KCl, 12.5 g of NH₄Cl, 7 g of CaCl₂ × 2H₂O, and 0.68 g of KH₂PO₄ per liter. The 1,0009 trace element SL-10 is prepared as described [26 ], and the vitamin solution is prepared as described previously [28 (link)], except concentrated to 2000×. Resazurin is dissolved in water to a concentration of 5 mg/ml. Uracil is dissolved in water to a concentration of 20 mM. Maltose, yeast extract, and casein hydrolysate are dissolved in water to a concentration of 10 % (w/v). LOD liquid medium contains 1× CbeI partial base salts, 1× trace minerals, 1× vitamins, 0.25 mg/l resazurin, and 5 g/l maltose, and when necessary, 40 μM uracil. Biomass media contain all these components, except that maltose is omitted, and biomass is added prior to bottling, to a concentration of 5 g/l. Transformation media contain all the same components as defined media, with the addition of 19 amino acid solution. LOC medium contains all the same components as LOD medium, with the addition of 1 g/l yeast extract and 2 g/l casein hydrolysate. Water used for preparing media was boiled and cooled to drive off dissolved oxygen. Media were reduced with 1 g/l cysteine HCl, buffered with 1 g/l, NaHCO₃, pH adjusted to 6.8 with 8 M NaOH, and degassed with argon. The final pH is 7.2. Solid media were prepared as described for liquid media, but the media was prepared as a 2× concentrate. This is added to an equal volume of 3 % agar solution (for a final 1.5 % agar) prior to pouring. Cells were either plated onto the solidified agar surface, then overlaid with 4 ml of 1.5 % agar, or suspended in the overlay and poured onto the plate surface.

Farkas J., Chung D., Cha M., Copeland J., Grayeski P, & Westpheling J. (2012). Improved growth media and culture techniques for genetic analysis and assessment of biomass utilization by Caldicellulosiruptor bescii. Journal of industrial microbiology & biotechnology, 40(1), 41-49.

Publication 2012

Anaerobic Preculture and Culture Media

Cited 15 times

Saccharomyces cerevisiae BY4741 was used and precultured anaerobically in YPD media with 2.0% glucose (w/v) (Wako, Osaka, Japan) at 30 °C for 72 h without shaking. YPD media was used for one litter of medium: 10 g of yeast extract (Bacto, MD, USA), 20 g of Pepton (Bacto), 20 g of glucose, and adjusted to pH 6.
The preculture and culture medium for C. cellulovorans 743B (ATCC 35296) and C. beijerinckii NCIMB8052 (ATCC 51743) was partially modified and used [5]. For one litter of medium, it was prepared with 4 g of yeast extract, 1 mg of Resazurin salt, 1 g of l-cysteine HCl, 5 g of NaHCO₃, 0.45 g of K₂HPO₄, 0.45 g of KH₂PO₄, 0.3675 g of NH₄Cl, 0.9 g of NaCl, 0.1575 g of MgCl₂∙6H₂O, 0.12 g of CaCl₂∙2H₂O, 0.85 mg of MnCl₂∙4H₂O, 0.942 mg of CoCl₂∙6H₂O, 5.2 mg of Na₂EDTA, 1.5 mg of FeCl₂∙4H₂O, 0.07 mg of ZnCl₂, 0.1 mg of H₂BO₃, 0.017 mg of CuCl₂∙2H₂O, 0.024 mg of NiCl₂∙6H₂O, 0.036 mg of Na₂MoO₄∙2H₂O, 6.6 mg of FeSO₄∙7H₂O, 0.1 g of p-aminobenzoic acid), and was adjusted to pH 7 for C. cellulovorans and to pH 5 for C. beijerinckii, respectively. C. cellulovorans and C. beijerinckii were anaerobically precultured in the above medium with 0.5% (w/v) cellobiose (Sigma, MO, USA) and with 2.0% (w/v) glucose, respectively, at 37 °C for 23 h without shaking.

Tomita H., Okazaki F, & Tamaru Y. (2019). Direct IBE fermentation from mandarin orange wastes by combination of Clostridium cellulovorans and Clostridium beijerinckii. AMB Express, 9, 1.

Full text: Click here

Publication 2019

4-Aminobenzoic Acid Bicarbonate, Sodium Cellobiose cupric chloride Cysteine Hydrochloride Glucose Magnesium Chloride manganese chloride Normal Saline potassium phosphate, dibasic resazurin Saccharomyces cerevisiae Sodium Chloride sodium molybdate(VI) Yeast, Dried

Most recents protocols related to «Resazurin»

Mycobacterium tuberculosis Starvation Assay

Mtb H37Rv strains were starved at an OD₆₀₀ of 1 for 2 or 7 weeks using the standard starvation media before it was diluted 10-fold in fresh starvation media containing 0.8 mg/mL oleic and 0.1 mg/mL resazurin. Absorbance was measured at 570 and 600 nm. Reduction grade for each time point was calculated using the following equation:
where ε is the molar extinction coefficient for resazurin depending on the wavelength and its oxidation state, A is the absorbance at a given wavelength, t₀ is the initial measurement, and t_x is the measurement at a given time x. The values for the extinction coefficient are the following: εOX_{_600nm} = 117.216, εOX_{_570nm} = 80.586, εRED_{_570nm} = 155.677, and εRED_{_600nm} = 14.652 [Biosystems, (80 )]. The experiments were performed in sextuplicate in black 96 well plates with clear flat bottom (Corning) in a final volume of 100 µL per well. The microtiter plates were incubated in a closed container with a damp towel at 37°C.

Sturm A., Sun P., Avila-Pacheco J., Clatworthy A.E., Bloom-Ackermann Z., Wuo M.G., Gomez J.E., Jin S., Clish C.B., Kiessling L.L, & Hung D.T. (2024). Genetic factors affecting storage and utilization of lipids during dormancy in Mycobacterium tuberculosis. mBio, 15(2), e03208-23.

Full text: Click here

Publication 2024

Resazurin-based cell viability assay

Resazurin reduction was used to assess the short-term effects of drug treatment. Sterile 0.15 mg/ml resazurin stock was prepared in PBS. Cells were seeded in 96-well plates in RPMI-1640 at least 24 hours before the experiment. The medium was replaced with fresh RPMI-1640 mixed with resazurin stock 1:6 and supplemented with a drug or vehicle control. Cells were incubated for 90 min, and resorufin fluorescence was measured (560 nm excitation and 590 nm emission). The resazurin reduction assay was not used as a proxy for cell count in the present study.

Mukha D., Dessain J., O’Connor S., Pniewski K., Bertolazzi F., Patel J., Mullins M, & Schug Z.T. (2024). Identification of Fasnall as a therapeutically effective Complex I inhibitor. bioRxiv.

Full text: Click here

Publication Preprint 2024

Resazurin Reduction Assay for Cytotoxicity

Not available on PMC !

We performed the resazurin reduction assay in order to examine the cytotoxicity of the extracts. The assay depends on the reduction of resazurin to resorufin by viable cells [30] (link). When non-viable cells lose their metabolic capacity hindering resorufin generation from resazurin, they do not show a blue staining. Briefly, aliquots of 5×10 5 adherent cells were seeded in 96-well-plates and were allowed to attach overnight. In the following, the cells were incubated with or without adding variable concentrations of the test substance to obtain a final volume of 200 μl/well. After 72 h incubation and combining resazurin (Sigma-Aldrich, Türkiye) for 4 h, staining was determined by an Infinite 200 M Plex plate reader (Tecan, Türkiye) using an excitation wavelength of 544 nm and an emission wavelength of 590 nm. Each assay was independently performed thrice, with six parallel replicates each. The protocol has been recently reported [31] (link).

Özenver N., ERKMEN Y., BOYALI F., & Demirezer L.Ö. (2024). CYTOTOXICITY SCREENING AND ANTIOXIDANT CAPACITY ASSESSMENT OF THE INNER PERIANTH SEGMENTS OF 14 RUMEX SPECIES GROWN IN TÜRKİYE. Ankara Universitesi Eczacilik Fakultesi Dergisi, 48(2), 5-5.

Publication 2024

Resazurin Reduction Assay for Cell Viability

The resazurin reduction assay to determine cell viability and metabolism was performed according to Ivanov et al. (2014) (link) with modifications. In brief, a stock solution (600 µM in PBS) was aliquoted and stored at −18°C. On the day of analysis, a working solution of 60 µM resazurin in the DMEM/F12 medium was prepared and protected from light. After 24 h of treatment, the medium in the wells was replaced with a working solution of resazurin (100 µL), and the plates were again stored at 37°C. Absorbance was measured at 570 and 600 nm using a microplate reader (BioTek Instruments, United States) 30 min and 4 h after the addition of the dye.

Kamińska J., Hecel A., Słowik J., Rombel-Bryzek A., Rowińska-Żyrek M, & Witkowska D. (2024). Characterization of four peptides from milk fermented with kombucha cultures and their metal complexes—in search of new biotherapeutics. Frontiers in Molecular Biosciences, 11, 1366588.

Full text: Click here

Publication 2024

Resazurin-Based Viability Assay

A 6× solution of resazurin sodium salt (Sigma–Aldrich, R7017) resuspended in Dulbecco’s phosphate-buffered saline was added to a final concentration of 25 μg/ml in fresh DMEM and then added to multiwell plates. After a 2 h incubation at 37°C, fluorescent signal (560 nm excitation/590 nm emission) was measured using a BioTek Synergy HT plate reader. Values for wells with no cells (i.e. media only) were subtracted for each well prior to further data processing.

Schillo J.L., Feddersen C.R., Peplinski R.M., Powell L.S., Varzavand A., Stipp C.S., Riordan J.D, & Dupuy A.J. (2024). Single-cell genomics analysis reveals complex genetic interactions in an in vivo model of acquired BRAF inhibitor resistance. NAR Cancer, 6(1), zcad061.

Full text: Click here

Publication 2024

Top products related to «Resazurin»

Resazurin by Merck Group

Sourced in United States, Germany, United Kingdom, Portugal, Australia, Italy, Canada, India, Brazil, France, Belgium, Poland, Sweden, Spain, China, Netherlands

Resazurin is a redox-sensitive dye that can be used as a non-toxic indicator in cell viability and proliferation assays. It undergoes a color change from blue to pink upon reduction, which can be measured using spectrophotometric or fluorometric methods.

Resazurin sodium salt by Merck Group

Sourced in United States, Germany, United Kingdom, Switzerland, Australia, Canada, Brazil

Resazurin sodium salt is a chemical compound commonly used as an indicator in various laboratory applications. It is a redox-sensitive dye that changes color based on the oxidation-reduction state of the surrounding environment. When used in cell culture systems, resazurin can be employed as a viability and cytotoxicity assay to measure cellular metabolic activity.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

Alamar blue by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, France, Canada, Portugal, Italy, Belgium, Ireland, Australia, India, Switzerland, China, Spain, Hungary, Japan, Denmark

Alamar Blue is a cell viability indicator used to measure the proliferation and cytotoxicity of cells in various laboratory applications. It is a non-toxic reagent that can be added directly to cell cultures and provides a quantitative measure of cell health and metabolic activity.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Resazurin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China

Resazurin is a non-toxic, non-fluorescent compound that becomes fluorescent and red-colored when reduced by metabolically active cells. It is commonly used as a cell viability indicator in various cell-based assays.

Prestoblue by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, Japan, Canada, Australia, Switzerland, Ireland

PrestoBlue is a cell viability reagent used to assess the metabolic activity of cells. It is a resazurin-based solution that undergoes a color change when reduced by viable cells, allowing for the quantification of cell growth and proliferation.

Resazurin solution by Merck Group

Sourced in United States, Germany, Italy

Resazurin solution is a laboratory reagent commonly used as an indicator for cellular metabolic activity. It is a blue, non-fluorescent dye that is converted to a pink, highly fluorescent dye (resorufin) by metabolically active cells. This color change can be used to assess cell viability and proliferation in various cell-based assays.

Celltiter blue by Promega

Sourced in United States, Germany, United Kingdom, Sweden

CellTiter-Blue is a cell viability assay kit that measures the metabolic activity of cells. It uses the indicator dye, resazurin, to quantitatively measure the number of viable cells in a sample.

Spectramax m5 by Molecular Devices

Sourced in United States, China, Japan, Switzerland, United Kingdom, Canada, Germany, Brazil, Uruguay

The SpectraMax M5 is a multi-mode microplate reader designed for absorbance, fluorescence, and luminescence measurements. It provides a diverse range of capabilities to support various applications in life science research and drug discovery.

What is Resazurin and how is it used in research?

What are some common challenges in using Resazurin assays?

How can PubCompare.ai help optimize Resazurin assay protocols?

PubCompare.ai allows researchers to more efficiently screen protocol literature and leverage AI to pinpoint critical insights. The platform can help identify the most effective Resazurin assay protocols for your specific research goals. Its AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy. This can help ensure your Resazurin assays are well-designed and produce reliable results.

What are some of the different applications of Resazurin assays?

Resazurin assays have a wide range of applications in research. They are commonly used to evaluate cell proliferation, cytotoxicity, and metabolic activity in drug discovery, toxicology, and cell biology studies. Resazurin is also used to assess microbial viability and contamination in food and water samples. The versatility of this dye makes it a valuable tool across many scientific disciplines.

More about "Resazurin"

Resazurin, also known as Resazurin sodium salt or Alamar Blue, is a blue, non-fluorescent dye that can be reduced to the pink, highly fluorescent resorufin by metabolically active cells.
This reduction process is commonly used in cell viability assays, as the amount of resorufin produced is proportional to the number of live cells.
Resazurin-based assays, such as PrestoBlue and CellTiter-Blue, are widely used in drug discovery, toxicology, and cell biology research to evaluate cell proliferation, cytotoxicity, and metabolic activity.
The PubCompare.ai tool can help researchers optimize their Resazurin assay protocols by providing data-driven insights to identify the best procedures and products for their experiments, ensuring reproducibility and accuracy.
With its AI-driven capabilities, PubCompare.ai allows users to easily locate and compare Resazurin assay protocols from literature, pre-prints, and patents, leveraging this valuable information to improve the quality and efficiency of their research.
When conducting Resazurin assays, it's important to consider factors such as DMSO, which can be used to solubilize the dye, and FBS, which can impact cell growth and metabolism.
By using the SpectraMax M5 or similar plate readers, researchers can accurately measure the fluorescence of the reduced resorufin, providing quantitative data on cell viability and metabolic activity.
By optimizing their Resazurin assay protocols with the help of PubCompare.ai, researchers can ensure more reliable and reproducible results in their cell biology, drug discovery, and toxicology studies.