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Resveratrol

Resveratrol is a polyphenolic compound found naturally in various plants, including grapes, berries, and peanuts.
It has generated significant interest in the scientific community due to its potential health benefits, including antioxidant, anti-inflammatory, and cardioprotective properties.
Reserach has sugested that resveratrol may have therapeutic applications in a variety of conditions, such as cardiovascular disease, neurodegenerative disorders, and cancer.
This MeSH term provides a comprehensive overview of the current understanding of resveratrol and its potential role in health and disease.

Most cited protocols related to «Resveratrol»

1
Multiplex Assay for Neurodegenerative Markers
Cited 11 times
We used a multiplex Xmap technology that uses magnetic microspheres internally coded with two fluorescent dyes to measure markers of neurodegeneration (Millipore, Cat#: HNABTMAG-68K). All samples including placebo and resveratrol at baseline and 52 weeks were analyzed in parallel using the same reagents. Through precise combinations of these two dyes, multiple proteins are measured within the sample. Each of these spheres is coated with a specific capture antibody. The capture antibody binds to the detection antibody and a reporter molecule, completing the reaction on the surface of the bead. CSF or plasma (25 μl) was incubated overnight at 4 °C with 25 μl of a mixed bead solution, containing human total tau, p-tau181, Aβ42, and Aβ40 (CSF Aβ40 is diluted 1:10). After washing, samples were incubated with 25 μl detection antibody solution for 1.5 h at room temperature. Streptavidin-phycoerythrin (25 μl) was added to each well containing the 25 μl of detection antibody solution. Samples were then washed and suspended in 100 μl of sheath fluid. Samples were then run on MAGPIX with Xponent software. The median fluorescent intensity (MFI) data was analyzed using a 5-parameter logistic or spline curve-fitting method for calculating analyte concentrations in samples. We also performed multiplex ELISA (Millipore, CAT#: HCYTOMAG-60K) to profile a panel of plasma and CSF markers that are indicative of inflammation, including human EGF, FGF-2, Eotaxin, TGF-α, G-CSF, Flt-3L, GM-CSF, Fractalkine, IFNα2, IFNγ, GRO, IL-10, MCP-3, IL-12P40, MDC, IL-12P70, PDGF-AA, IL-13, PDGF-AB/BB, IL-15, sCD40L, IL-17A, IL-1RA, IL-1α, IL-9, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, TNFα, TNFβ, and VEGF.
Moussa C., Hebron M., Huang X., Ahn J., Rissman R.A., Aisen P.S, & Turner R.S. (2017). Resveratrol regulates neuro-inflammation and induces adaptive immunity in Alzheimer’s disease. Journal of Neuroinflammation, 14, 1.
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Publication 2017
Becaplermin CCL2 protein, human CCL7 protein, human Dyes Enzyme-Linked Immunosorbent Assay Eotaxin-1 Fibroblast Growth Factor 2 Fluorescent Dyes Fractalkine Granulocyte-Macrophage Colony-Stimulating Factor Granulocyte Colony-Stimulating Factor Homo sapiens IL1RN protein, human IL10 protein, human Immunoglobulins Inflammation Interferon Type II Interleukin-1 beta Interleukin-12 Interleukin-12 Subunit p40 Interleukin-13 Interleukin-15 Interleukin-17A Microspheres Nerve Degeneration PDGF AA Phycoerythrin Placebos Plasma Proteins RANTES Resveratrol Streptavidin TGFA protein, human Tumor Necrosis Factor-alpha TUMOR NECROSIS FACTOR BETA Vascular Endothelial Growth Factors
2
Cell Viability Assay of Resveratrol and Ascorbic Acid
Cited 10 times
The neutral red assay [23 (link),24 (link)] was used to determine the cell viability after incubation with the different test compounds. HUH7 cells were seeded at a density of 0.2 × 106 cells/well (for HUH7) or 0.15 × 106 cells/well (for HUH7/PON1) for 24 h and treated with 5-100 μmol/l resveratrol and 10-1000 μmol/l ascorbic acid, respectively. HUH7 cells were incubated with test compounds for 24 h, HUH7/PON1 cells for 48 h. In brief, the culture medium containing the test substances was replaced with fresh serum-containing medium including 60 μg/ml of Neutral Red (Carl Roth, Karlsruhe, Germany). After incubation for 1.5 h the medium was removed and the cells were extracted using a solution comprising 50:49:1 (v/v/v) ethanol, water and glacial acetic acid. The absorbance was measured in a plate reader (Labsystems, Helsinki, Finland) at 540 nm.
Wagner A.E., Boesch-Saadatmandi C., Breckwoldt D., Schrader C., Schmelzer C., Döring F., Hashida K., Hori O., Matsugo S, & Rimbach G. (2011). Ascorbic acid partly antagonizes resveratrol mediated heme oxygenase-1 but not paraoxonase-1 induction in cultured hepatocytes - role of the redox-regulated transcription factor Nrf2. BMC Complementary and Alternative Medicine, 11, 1.
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Publication 2011
Acetic Acid Ascorbic Acid Biological Assay Cells Cell Survival Ethanol PON1 protein, human Resveratrol Serum
3
Rhesus Monkeys on HFS Diet
Cited 9 times

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Publication 2014
Animals Dietary Supplements Macaca mulatta Males Monkeys Placebos Resveratrol Sucrose Therapy, Diet
4
Reactive Gliosis and Resveratrol Effects
Cited 9 times
For reactive gliosis 1.0 μl of 2mM domoic acid and 7mM ouabain plus 1mM BrdU was injected into the eyes of P16 ketamine/xylazine anesthetized mice 16 (link). For resveratrol, time pregnant female mice were injected daily with 4 mg/kg of body weight (Cat. 60512A, AKSci) or 0.1%DMSO intraperitoneally from E16. Virus injection and electroporation were as described 30 . Fixation, antibody labeling, TUNEL, and quantification of sections, or dissociation and quantification of cells, and RNA and protein analysis were essentially as described 15 (link), 31 (link). The PALM microlaser system was used for LCM following the manufacturer’s recommendations. For flow cytometry E2f3f/f and E2f1−/−, E2f2−/−, E2f3f/f fibroblasts were infected with MXIE or MXIRE-Cre retrovirus. E14 retinas were dissected and dissociated. GFP+ cells were sorted using a FACSAria (Becton Dickinson). For cell cycle analysis, cells were fixed in ice-cold 80% ethanol, counterstained with propidium iodide and analyzed with a BD FACSCaliburTM system. Data were collected using CELLFIT software.
Full Methods and associated references are available in the online version of the paper.
Chen D., Pacal M., Wenzel P., Knoepfler P.S., Leone G, & Bremner R. (2009). Division and apoptosis in the E2f-deficient retina. Nature, 462(7275), 925.
Publication 2009
Arecaceae Body Weight Bromodeoxyuridine Cell Cycle Cells Common Cold domoic acid E2F1 protein, human Electroporation Ethanol Eye Fibroblasts Flow Cytometry Gliosis Immunoglobulins In Situ Nick-End Labeling Ketamine Mus Ouabain Pregnant Women Propidium Iodide Proteins Resveratrol Retina Retroviridae Sulfoxide, Dimethyl Virus Xylazine
5
Cisplatin-Induced Neuropathy Protection
Cited 7 times
Cisplatin (Tocris, Ellisville, MO, USA) was administered intraperitoneally (i.p.) once a week at a dose of 5 mg/kg for 6 (36 days) weeks (cumulative dose: 30 mg/kg i.p.) [5 (link)]. Cisplatin was diluted in normal saline (0.9% NaCl) and delivered in a volume of 10 ml/kg body weight. Control groups were injected with an equivalent volume of saline (i.p.) in lieu of Cisplatin
[8 (link)]. Before each Cisplatin/saline i.p. injection either (1) 0.9% NaCl
[15 (link)]; (2) 4% sodium bicarbonate (NaHCO3 dissolved in 0.9% NaCl)
[8 (link)]; (3) vitamin C (25 mg/kg s.c.)
[22 (link)]; or (4) resveratrol (25 mg/kg s.c.)
[20 (link)] was administered subcutaneously in a final volume of 1 ml. Injections were always performed after completion of mechanical and cold withdrawal testing.
Guindon J., Deng L., Fan B., Wager-Miller J, & Hohmann A.G. (2014). Optimization of a cisplatin model of chemotherapy-induced peripheral neuropathy in mice: use of vitamin C and sodium bicarbonate pretreatments to reduce nephrotoxicity and improve animal health status. Molecular Pain, 10, 56.
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Publication 2014
Ascorbic Acid Bicarbonate, Sodium Body Weight Cisplatin Common Cold Normal Saline Resveratrol Saline Solution

Most recents protocols related to «Resveratrol»

1
Resveratrol and SEB Protocol
Resveratrol (RES) was purchased from Supelco (MO, United States) and prepared freshly in an appropriate vehicle (VEH) consisting of 1% carboxyl methyl cellulose (CMC, Sigma-Aldrich, United States). SEB was obtained from Toxin Technology Inc. (FL, United States) and aliquoted in sterile phosphates buffer saline (PBS) at a stock concentration of 2 μg/μL prior to storage at −20°C until being used.
Alghetaa H., Mohammed A., Singh N., Wilson K., Cai G., Putluri N., Nagarkatti M, & Nagarkatti P. (2023). Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway. Frontiers in Pharmacology, 14, 1106733.
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Publication 2023
Buffers Methylcellulose Phosphates Resveratrol Saline Solution Sterility, Reproductive Toxins, Biological
2
Resveratrol Reverses SEB-Induced ARDS
We used a previously described method to induce ARDS by SEB involving double sensitization with SEB in C3H/HeJ mice which leads to 100% mortality and treatment with RES reverses this action. Briefly, C3H/HeJ mice pretreated orally with two doses of either 100 mg/kg resveratrol or VEH at 24 h intervals. After the second dose of RES, intranasal administration of 5µg/mouse of SEB was given and then after 90 min from the first SEB dose, a second dose of i. p 2µg/mouse of SEB was given as a fatal model of dual-dose SEB exposure (Rao et al., 2015b (link); Alghetaa et al., 2018 (link)). Endpoint of the experiment was 48 h post first SEB exposure at which time the mice were euthanized and the infiltrating mononuclear cells in the lungs were collected by gradient separation (Elliott et al., 2016 (link)).
Alghetaa H., Mohammed A., Singh N., Wilson K., Cai G., Putluri N., Nagarkatti M, & Nagarkatti P. (2023). Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway. Frontiers in Pharmacology, 14, 1106733.
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Publication 2023
Administration, Intranasal Lung Mice, Inbred C3H Mus Respiratory Distress Syndrome, Adult Resveratrol
3
Single-cell RNA-seq of Lung Cells
The TC20 Automated Cell Counter (BioRad) was used to measure the cell count and viability of isolated lung cells. The cells were next loaded onto the Chromium Controller (10x Genomics). Following the manufacturer’s protocol, the Chromium single cell 5′ reagent kits (10x Genomics) were used to process samples into single-cell RNA-seq (scRNAseq) libraries. Sequencing of those libraries was performed using the NextSeq 550 instrument (Illumina) with a depth of 40k–60k reads per cell. The base call files generated from sequencing the libraries were then processed in the 10x Genomics Cell Ranger pipeline (version 2.0) to create FASTQ files. The FASTQ files were then aligned to the mm10 mouse genome and the read count for each gene in each cell was generated (Mohammed et al., 2020c (link)). Downstream analysis was completed using Seurat suite version 3.0 within R studio (Butler et al., 2018 (link); Stuart et al., 2019 (link)). Data were integrated into Seurat using anchor and integration functions. The integrated data were scaled and Principal-component analysis (PCA) was completed for dimensionality reduction. Clusters were made following PCA analysis by adjusting the granularity resolution to 0.25. We determined the number of principal components (PCs) to utilize post-JackStraw analysis within Seurat to determine PCs with the lowest p-value. Differential expressions were determined for each cluster to determine cluster biomarkers, and between the vehicle and resveratrol-treated samples using the default Wilcoxon rank sum test.
Alghetaa H., Mohammed A., Singh N., Wilson K., Cai G., Putluri N., Nagarkatti M, & Nagarkatti P. (2023). Resveratrol attenuates staphylococcal enterotoxin B-activated immune cell metabolism via upregulation of miR-100 and suppression of mTOR signaling pathway. Frontiers in Pharmacology, 14, 1106733.
Full text: Click here
Publication 2023
Biological Markers Cells Cell Survival Chromium Cytoplasmic Granules Genes Genome Lung Mus Resveratrol Single-Cell RNA-Seq
4
Constructing Resveratrol-Producing Yeast Strains
For the construction of resveratrol-producing strains, Pc4CL (P14912.1) from Petroselinum crispum, VvSTS (P28343.2) from Vitis vinifera, RtPAL/TAL (P11544) from Rhodotorula toruloides (Synonyms R. gracilis), AtCPR1 (NP_194183.1) and AtC4H (NP_180607.1) from Arabidopsis thaliana were codon-optimized for expression in S. cerevisiae and synthesized by Sangon Biotech (Shanghai, China). SeACSL641P (MP052228.1) and selective marker cassettes (HIS3, LEU2 and URA3) were also synthesized by Sangon Biotech (Shanghai, China). Integration homologous arms (about 500 bp), ScACC1, ScARO4, ScARO7, and ScARO2 were amplified from the genome of BY4742. LYS2 including its homologous arms was amplified from the genome of W303-1A. EcAROL was amplified from the genome of E. coli. Mutants of ACC1, ARO4 and ARO7 were created by overlap PCR according to the previous report. Expression cassettes with promoters, terminators and genes were assembled to plasmid G418 using Minerva Super Fusion Cloning Kit (Yuheng Biotech, Suzhou, China). The plasmids pCas with specific gRNA sequences used for CRISPR/Cas9 editing were obtained according to the standard Quick-Change Site-Directed Mutagenesis protocol. The gRNA sequences of sites cit2, lpp1, pha2, and dpp1 were designed using online tool E-CRISP [37 (link)], and the gRNA sequences of other sites were reported in the previous study [38 (link)]. Recombinant plasmids were confirmed by DNA sequencing. The detailed information of plasmids (Additional file 1: Table S2) and the primers (Additional file 1: Table S3) used in our study were listed in supplementary information.
Meng L., Diao M., Wang Q., Peng L., Li J, & Xie N. (2023). Efficient biosynthesis of resveratrol via combining phenylalanine and tyrosine pathways in Saccharomyces cerevisiae. Microbial Cell Factories, 22, 46.
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Publication 2023
2-(5-(3-fluorophenyl)-1H-pyrazol-3-yl)-5-methylphenol ACACA protein, human antibiotic G 418 Arabidopsis thalianas Arm, Upper Clustered Regularly Interspaced Short Palindromic Repeats Codon Escherichia coli Genes Genome Gracilis Muscle Mutagenesis, Site-Directed Oligonucleotide Primers Petroselinum crispum Plasmids Resveratrol Rhodotorula toruloides Saccharomyces cerevisiae Strains Vitis
5
Optimizing Resveratrol Production in Yeast
Single colonies were picked from the YPD agar plate, inoculated into 5 mL YPD in culture tubes, and incubated overnight at 30 °C in a rotary shaker (200 rpm). The seed cultures were then transferred into 50 mL fresh medium to reach an initial OD600 of 0.1–0.2 and grown under the same conditions. The fermentation ended at 72 h and 144 h respectively when strains cultured in YPD containing 20 g/L glucose (YPD-20G) and 40 g/L glucose (YPD-40G) to compare the performance of different strains. Time courses were plotted every 12 h or 24 h until resveratrol titer reached a plateau. Cell growth was evaluated by OD600 using a Shimadzu UV-1900i spectrophotometer, and dry cell weight (DCW) in shake flasks was calculated using the coefficient, 1 OD600 = 0.3722 g/L DCW.
Meng L., Diao M., Wang Q., Peng L., Li J, & Xie N. (2023). Efficient biosynthesis of resveratrol via combining phenylalanine and tyrosine pathways in Saccharomyces cerevisiae. Microbial Cell Factories, 22, 46.
Full text: Click here
Publication 2023
Agar Cells Fermentation Gastrin-Secreting Cells Glucose Resveratrol Strains Tremor

Top products related to «Resveratrol»

1
Resveratrol by Merck Group
Sourced in United States, Germany, Italy, Sao Tome and Principe, France, China, Canada, Spain, United Kingdom, Poland, Japan, India, Macao, Argentina
Resveratrol is a naturally occurring polyphenolic compound found in various plants, including grapes and berries. It is commonly used as a dietary supplement and in laboratory research settings. Resveratrol has been studied for its potential antioxidant and anti-inflammatory properties, but its specific functions and applications should be evaluated based on scientific evidence.
2
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Quercetin by Merck Group
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
5
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
6
Epicatechin by Merck Group
Sourced in United States, Germany, Italy, France, China, Spain, United Kingdom, Australia, Switzerland, Belgium, Sao Tome and Principe, India, New Zealand
Epicatechin is a natural compound found in various plants and is commonly used in laboratory settings. It serves as a standard reference material for analytical and research purposes. Epicatechin exhibits antioxidant properties and is often employed in the evaluation of antioxidant activity and the development of analytical methods.
7
Caffeic acid by Merck Group
Sourced in United States, Germany, Italy, France, Poland, Spain, China, United Kingdom, Australia, Sao Tome and Principe, Switzerland, India, Ireland, Canada, Macao, Brazil, Austria, Mexico, Czechia, Portugal
Caffeic acid is a phenolic compound commonly found in various plants. It serves as a laboratory standard for the identification and quantification of similar phenolic compounds using analytical techniques such as high-performance liquid chromatography (HPLC) and spectrophotometry.
8
Catechin by Merck Group
Sourced in United States, Germany, Italy, France, Australia, India, Spain, United Kingdom, China, Poland, Sao Tome and Principe, Japan, Portugal, Canada, Switzerland, Brazil, Malaysia, Singapore, Macao, Belgium, Ireland, Mexico, Hungary
Catechin is a natural polyphenolic compound found in various plants, including green tea. It functions as an antioxidant, with the ability to scavenge free radicals and protect cells from oxidative stress.
9
P coumaric acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, China, Poland, United Kingdom, Sao Tome and Principe, Switzerland, Canada, Ireland, India, Australia, Japan, Macao, Portugal
P-coumaric acid is a naturally occurring phenolic compound that can be utilized as a reference standard or an analytical reagent in various laboratory settings. It is a white to off-white crystalline solid that is soluble in organic solvents. P-coumaric acid is commonly used as a standard in analytical techniques, such as high-performance liquid chromatography (HPLC) and spectrophotometric measurements, to quantify and characterize similar compounds in sample matrices.
10
Rutin by Merck Group
Sourced in United States, Germany, Italy, France, China, Spain, India, Australia, Poland, United Kingdom, Sao Tome and Principe, Ireland, Brazil, Portugal, Canada, Switzerland, Japan
Rutin is a laboratory reagent used for analytical and research purposes. It is a flavonoid compound derived from various plant sources. Rutin exhibits antioxidant and anti-inflammatory properties, and is commonly used in assays, chromatography, and other analytical techniques.

More about "Resveratrol"

Resveratrol, a naturally occurring polyphenolic compound found in various plants like grapes, berries, and peanuts, has generated significant scientific interest due to its potential health benefits.
This powerful antioxidant and anti-inflammatory agent has shown promising therapeutic applications in a variety of conditions, including cardiovascular disease, neurodegenerative disorders, and cancer.
Resveratrol, also known as trans-3,4',5-trihydroxystilbene, is a member of the stilbene family of phytoalexins.
These compounds are produced by plants as a defense mechanism against environmental stressors, such as ultraviolet radiation, pathogens, and mechanical injury.
The potential health benefits of resveratrol have been extensively studied, with research suggesting that it may help protect the cardiovascular system, improve neurological function, and potentially inhibit the growth of certain cancer cells.
In addition to its antioxidant and anti-inflammatory properties, resveratrol has also been linked to improved insulin sensitivity and reduced risk of type 2 diabetes.
Closely related compounds, such as DMSO, quercetin, gallic acid, epicatechin, caffeic acid, catechin, and p-coumaric acid, have also been the focus of scientific investigation for their own unique health-promoting effects.
These polyphenolic compounds often work synergistically, and their combined use may enhance the overall therapeutic potential.
To optimize your resveratrol research, consider utilizing AI-powered platforms like PubCompare.ai.
These advanced tools can help you locate relevant protocols from the literature, preprints, and patents, and perform AI-driven comparisons to identify the most effective approaches.
By leveraging these cutting-edge technologies, you can enhance the reproducibility and accuracy of your resveratrol studies, taking your research to new heights.