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Rhein

Rhein is a naturally occurring anthraquinone derivative found in various medicinal plants, such as rhubarb and aloe vera.
It has been studied for its potential therapeutic properties, including anti-inflammatory, antioxidant, and laxative effects.
Rhein has been investigated for its role in managing conditions like constipation, liver disorders, and certain types of cancer.
Researchers can leverage the AI-driven platform PubCompare.ai to optimize their Rhein research protocols, enhancing reproducibility and accuracy.
The platform helps locate relevant protocols from literature, pre-prints, and patents, while providing AI-driven comparisons to identify the best protocols and products.
This can help improve the quality and efficiency of Rhein research.
Aditionally, PubCompare.ai offers powerful tools and insights to further advance Rhein-related investigations.

Most cited protocols related to «Rhein»

The above decomposition (Equation 2) suggests the following simple strategy for statistical learning of causal networks. First, by multiple testing of A MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=bq8bbaa@382B@ = 0 we determine the network topology, i.e. we identify those edges for which the corresponding partial correlation is not vanishing. Second, by subsequent multiple testing of log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) = 0 we establish a partial ordering of the nodes, which in turn imposes a partial directionality upon the edges.
In more detail, we propose the following five-step algorithm:
1. First, it is essential to determine an accurate and positive definite estimate R of the correlation matrix. Only if the sample size is large with many more observations than variables (n > > p) the usual empirical correlation estimate will be suitable. In all other instances, the use of a regularized estimator is absolutely vital (e.g., the Stein-type shrinkage estimator of [20 ]) in order to improve efficiency and to guarantee positive definiteness. In addition, if the samples are longitudinal it may be necessary to adjust for autocorrelation [27 ].
2. From the estimated correlations we compute the partial variances and correlations (see Table 1), and from those in turn plug-in estimates of the factors A MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=bq8bbaa@382B@ and MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ of Equation 2 for all possible edges. Note that in this calculation each variable assumes in turn the role of the response Y . An efficient way to calculate the various MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ is given by taking the square root of the diagonal of the inverse of the estimated correlation matrix, and computing the corresponding pairwise ratios.
3. Subsequently, we infer the partial correlation graph following the algorithm described in [19 (link)]. Essentially, we perform multiple testing of all partial correlation coefficients A MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=bq8bbaa@382B@ . Note that for high dimensions (large p) the null distribution of partial correlations across edges can be determined from the data, which in turn allows the adaptive computation of corresponding false discovery rates [28 (link)].
4. In a similar fashion we then conduct multiple testing of all log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ). As MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ is the ratio of two variances with the same degrees of freedom, it is implicit that log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) is approximately normally distributed [29 ], with an unknown variance parameter θ. Thus, the observed z = log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) across all edges follow a mixture distribution

Assuming that most z belong to the null model, i.e. that most edges are undirected, it is possible to infer non-parametrically the alternative distribution fA (z), the proportion η0, as well as the variance parameter θ – for an algorithm see [28 (link)]. From the resulting densities and distribution functions local and tail-area-based false discovery rates for the test log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) = 0 are computed. Note that in this procedure we include all edges, regardless of the corresponding value of A MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=bq8bbaa@382B@ or the outcome of the test A MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=bq8bbaa@382B@ = 0.
5. Finally, a partially directed network is constructed as follows. All edges in the correlation graph with significant log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) ≠ 0 are directed in such a fashion that the direction of the arrow points from the node with the larger standardized partial variance (the more "exogenous" variable) to the node with the smaller standardized partial variance (the more "endogenous" variable). The other edges with log( MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaat0uy0HwzTfgDPnwy1egaryqtHrhAL1wy0L2yHvdaiqaaliab=Xsicbaa@3788@ ) ≈ 0 remain undirected. The subgraph consisting of all directed edges constitutes the inferred causal network. Note that this does not necessarily include all nodes that are contained in the GGM network.
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Publication 2007
Acclimatization Plant Roots Tail
We enrolled patients at Mulago Hospital, Kampala, and Mbarara Hospital, Mbarara — both in Uganda — and at GF Jooste Hospital in Cape Town, South Africa, beginning in November 2010, February 2011, and April 2011, respectively. Patients with suspected meningitis were screened at the time of hospital presentation and counseled regarding cryptococcosis, HIV and AIDS, ART, and possible research participation. Eligibility criteria for enrollment were an age of 18 years or older, a diagnosis of human immunodeficiency virus (HIV) infection, no previous receipt of ART, a diagnosis of cryptococcal meningitis based on cerebrospinal fluid (CSF) culture or CSF cryptococcal antigen assay, and treatment with amphotericin-based therapy. Exclusion criteria were an inability to undergo follow-up, contraindication for or refusal to undergo lumbar punctures, multiple concurrent CNS infections, previous cryptococcosis, receipt of chemotherapy or immunosuppressive agents, pregnancy, breast-feeding, and serious coexisting conditions that precluded random assignment to earlier or deferred ART. Women included in the study agreed to use two forms of contraception, because high-dose fluconazole is potentially teratogenic during the first trimester of pregnancy. Written informed consent was obtained from each participant or his or her surrogate. The institutional review board at each participating site approved the study.
Publication 2014
Acquired Immunodeficiency Syndrome Amphotericin Antigens Biological Assay Central Nervous System Infection Cerebrospinal Fluid Contraceptive Methods Cryptococcus Cryptococcus neoformans Infections Diagnosis Eligibility Determination Ethics Committees, Research Fluconazole HIV HIV Infections Immunosuppressive Agents Inpatient Meningitis Meningitis, Cryptococcal Patients Pharmacotherapy Pregnancy Punctures, Lumbar Teratogenesis Therapeutics Woman
H. polymorpha open reading frame sequences were collected from the H. polymorpha genome database (Rhein Biotech, unpublished; [7 (link)] and NCBI [45 (link)-47 (link)]. For the genes on contigs 1 - 48, the annotation was based on the information from RheinBiotech and Ramezani-Rad et al. [7 (link)]. The additional NCBI sequences were listed as Hp50 and Hp51 numbers. The annotation of these genes was as described on NCBI (Hp50s) or by manual blast search (Hp51s). All Hp sequences were applied for design of oligonucleotide probes using OLIGOARRAY v2.1 [48 (link)] with the following oligonucleotide primer design parameters: a length of 58-60 nucleotides, a melting temperature (Tm) of at least 80°C, a G/C-content of 34-52% and a maximum Tm of secondary structures and cross-hybridisations of 68°C. Oligo's were designed within the 3'-regions of the ORFs (setting: maximum distance between the 5' end of the oligo and the 3' end of the input sequence: 600-nt) to minimise including intron-sequences, since these were not discarded in the input ORF sequences. Paralogous sequences were removed during the final analysis of the design using blastN. Of the 6,248 oligo-probes, 6,002 are from the annotated genes of H. polymorpha and 23 probes are from heterologous genes of specific research interest (data not shown). The remaining 223 probes include positive and negative controls. Subsequently, the oligo-set was printed twice in each of the 8 arrays per slide (8-plex format) using Agilent's SurePrint technology (in situ synthesis; via eArray 4.0-website; Agilent Technologies Netherlands B.V., Amstelveen, the Netherlands).
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Publication 2010
Anabolism Crossbreeding Gene Annotation Genes Genome Introns Nucleotides Oligonucleotide Primers Oligonucleotide Probes Oligonucleotides Open Reading Frames rhein

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Publication 2019
Adult Amphotericin B Biological Assay Cryptococcus Diagnosis Ethics Committees, Research HIV Infections Hospital Referral Icterus Liver Cirrhosis Meningitis, Cryptococcal Outpatients Patients Placebos Punctures, Lumbar Sertraline
The Gutenberg Heart Study (GHS) is designed as a community-based, prospective, observational single-center cohort study in the Rhein-Main region in western mid-Germany. The primary aim of the study is to improve the individual cardiovascular risk prediction by identifying genetic and non genetic risk factors contributing to cardiovascular diseases, with a strong emphasis on atherosclerosis.
A sample of eligible participants was randomly drawn from the registers of the local registry offices in the city of Mainz and the district of Mainz-Bingen. This sample was stratified in a ratio of 1∶1 for gender and residence, and in equal numbers for decades of age. Inclusion criteria were an age between 35 and 74 years and a written consent; exclusion criteria were insufficient knowledge of the German language to understand explanations and instructions, and physical or psychic inability to participate in the examinations in the study center. Individuals were invited for a 5-hour baseline-examination to the study center where clinical examinations and collection of blood samples were performed. The present analysis was based on an initial sample of 3,336 subjects successively enrolled into the GHS from April 2007 to April 2008. Genomic DNA was isolated from all participants. Monocyte RNA was isolated from half of the participants recruited each day to ensure rapid sample processing and isolation of total RNA. For approximately 1,500 study participants, both DNA and RNA were available.
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Publication 2010
Atherosclerosis Birth Cardiovascular Diseases Gender Genome Heart isolation Monocytes Physical Examination rhein Specimen Collections, Blood

Most recents protocols related to «Rhein»

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An accurately weighed quantity of Rhein reference standard (70mg, 98.9% Purity) was dissolved with 100 mL methanol HPLC to prepare the stock solution. Standard working solutions of Rhein were prepared by diluting the stock solution with methanol to obtain five solutions of varying concentrations ranging from 2.5 mcg/mL to 20 mcg/ mL. A standard curve was then prepared using the Rhein standard solutions.
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1mg of rhein was taken in 10mL of volumetric flask and was dissolved in 5 mL of acetonitrile and made upto 10ml with acetonitrile to give primary stock solution to produce concentration 100µg/ml. Dilutions were prepared out of primary stock solution in the concentration range 2 to 10µg/mL by pipetting 0.2, 0.4, 0.6, 0.8, 1ml and diluting each of them to 10ml with diluent. The absorbance was measured for each by UV spectrophotometer at λmax 430nm. The graph was plotted for absorbance vs concentration.
Publication 2024

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More about "Rhein"

Rhein, a naturally occurring anthraquinone derivative, has garnered significant attention for its potential therapeutic benefits.
Found in various medicinal plants like rhubarb (Rheum) and aloe vera, this bioactive compound has been extensively studied for its anti-inflammatory, antioxidant, and laxative properties.
Researchers can leverage the innovative AI-driven platform PubCompare.ai to optimize their Rhein-related research protocols, enhancing reproducibility and accuracy.
The platform empowers scientists to easily locate relevant protocols from academic literature, preprints, and patents, while providing AI-driven comparisons to identify the most effective protocols and products.
This can help improve the overall quality and efficiency of Rhein research.
PubCompare.ai also offers a suite of powerful tools and insights to further advance investigations into the therapeutic potential of this versatile compound.
Rhein has been investigated for its role in managing a variety of conditions, including constipation, liver disorders, and certain types of cancer.
Researchers can explore the synergistic effects of Rhein with other compounds, such as DMSO (dimethyl sulfoxide) and Metacam (meloxicam), to develop more effective treatment strategies.
Additionally, the platform can assist in the analysis of Rhein-related data using statistical software like GraphPad Prism 7.
To further enhance Rhein research, scientists may also consider utilizing complementary techniques, such as the use of Buscopan (hyoscine butylbromide) for its antispasmodic properties, Polyvinyl alcohol for its versatility in formulations, and Formic acid for its role in sample preparation.
The integration of TRIzol reagent can also aid in the extraction and purification of Rhein from plant sources.
By leveraging the powerful features of PubCompare.ai, researchers can streamline their Rhein-related investigations, optimize research protocols, and unlock new insights that can ultimately lead to the development of innovative therapeutic solutions.