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Rhodamine

Rhodamine: A group of fluorescent dyes commonly used in biochemical and biomedical research.
These dyes exhibit bright, photostable fluorescence and are often employed as tracers, labels, and probes in various applications such as flow cytometry, microscopy, and protein detection.
Rhodamine dyes can be conjugated to biomolecules like antibodies, nucleic acids, and proteins, enabling visualization and tracking of cellular processes.
Their versatility and favorable optical properties make them indispensable tools for researchers in the life sciences.

Most cited protocols related to «Rhodamine»

1
Comprehensive Neuronal Tracing and Characterization
Cited 170 times
For in situ hybridization analysis, cryostat sections were hybridized using digoxigenin-labeled probes [45 (link)] directed against mouse TrkA or TrkB, or rat TrkC (gift from L. F. Parada). Antibodies used in this study were as follows: rabbit anti-Er81 [14 (link)], rabbit anti-Pea3 [14 (link)], rabbit anti-PV [14 (link)], rabbit anti-eGFP (Molecular Probes, Eugene, Oregon, United States), rabbit anti-Calbindin, rabbit anti-Calretinin (Swant, Bellinzona, Switzerland), rabbit anti-CGRP (Chemicon, Temecula, California, United States), rabbit anti-vGlut1 (Synaptic Systems, Goettingen, Germany), rabbit anti-Brn3a (gift from E. Turner), rabbit anti-TrkA and -p75 (gift from L. F. Reichardt), rabbit anti-Runx3 (Kramer and Arber, unpublished reagent), rabbit anti-Rhodamine (Molecular Probes), mouse anti-neurofilament (American Type Culture Collection, Manassas, Virginia, United States), sheep anti-eGFP (Biogenesis, Poole, United Kingdom), goat anti-LacZ [14 (link)], goat anti-TrkC (gift from L. F. Reichardt), and guinea pig anti-Isl1 [14 (link)]. Terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) to detect apoptotic cells in E13.5 DRG on cryostat sections was performed as described by the manufacturer (Roche, Basel, Switzerland). Quantitative analysis of TUNEL+ DRG cells was performed essentially as described [27 (link)]. BrdU pulse-chase experiments and LacZ wholemount stainings were performed as previously described [46 (link)]. For anterograde tracing experiments to visualize projections of sensory neurons, rhodamine-conjugated dextran (Molecular Probes) was injected into single lumbar (L3) DRG at E13.5 or applied to whole lumbar dorsal roots (L3) at postnatal day (P) 5 using glass capillaries. After injection, animals were incubated for 2–3 h (E13.5) or overnight (P5). Cryostat sections were processed for immunohistochemistry as described [14 (link)] using fluorophore-conjugated secondary antibodies (1:1,000, Molecular Probes). Images were collected on an Olympus (Tokyo, Japan) confocal microscope. Images from in situ hybridization experiments were collected with an RT-SPOT camera (Diagnostic Instruments, Sterling Heights, Michigan, United States), and Corel (Eden Prairie, Minnesota, United States) Photo Paint 10.0 was used for digital processing of images.
Hippenmeyer S., Vrieseling E., Sigrist M., Portmann T., Laengle C., Ladle D.R, & Arber S. (2005). A Developmental Switch in the Response of DRG Neurons to ETS Transcription Factor Signaling. PLoS Biology, 3(5), e159.
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Publication 2005
Anabolism Animals Antibodies Apoptosis Bromodeoxyuridine Calbindins Calretinin Capillaries Cavia Cells Diagnosis Digoxigenin DNA Nucleotidylexotransferase Domestic Sheep Goat Immunohistochemistry In Situ Hybridization In Situ Nick-End Labeling LacZ Genes Lumbar Region Mice, House Microscopy, Confocal Molecular Probes Neurofilaments Neuron, Afferent Pulse Rate Rabbits Rhodamine rhodamine dextran Root, Dorsal Staining transcription factor PEA3 tropomyosin-related kinase-B, human
2
Fluorescent Imaging of Golgi Organization
Cited 151 times
Cells were cultured on glass coverslips (Matsunami) pre-coated with 10 µg/ml fibronectin (Sigma) and fixed with 4% (w/v) paraformaldehyde in PBS or BRB80 [80 mM Pipes (pH 6.8), 1 mM MgCl2, and 1 mM EGTA] for 10 min at room temperature. Fixed cells were stained with the respective antibodies, phalloidin conjugated with either Alexa Fluor 488 or rhodamine (Invitrogen), along with DAPI (Sigma) as described previously2 (link), 54 (link). In situ proximity ligation assay (PLA) was performed using Duolink kit (Olink Bioscience) according to the manufacturer’s instructions. After completion of the PLA reaction, samples were refixed with 4% (w/v) paraformaldehyde and incubated with Alexa Fluor-conjugated secondary antibodies (Life Technologies) to detect the individual proteins. Fluorescence images were obtained using a laser scanning confocal imaging system (LSM700, Carl Zeiss) and processed using the ImageJ software. Number of Golgi fragments was quantified by using the ImageJ particle analysis tool. Colocalization was examined using the ImageJ JACoP plugin64 (link) or Metamorph (Molecular Devices).
Nishita M., Park S.Y., Nishio T., Kamizaki K., Wang Z., Tamada K., Takumi T., Hashimoto R., Otani H., Pazour G.J., Hsu V.W, & Minami Y. (2017). Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness. Scientific Reports, 7, 1.
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Publication 2017
alexa fluor 488 Antibodies Biological Assay Cells DAPI Egtazic Acid Fluorescence FN1 protein, human Golgi Apparatus Ligation Magnesium Chloride Medical Devices paraform Phalloidine piperazine-N,N'-bis(2-ethanesulfonic acid) Proteins Rhodamine
3
Detecting Apoptosis and Oxidative Stress in Yeast
Cited 42 times
Electron microscopy, annexin V labeling, and DAPI staining were performed as described previously (Madeo et al., 1997 (link)). For the TdT-mediated dUTP nick end labeling (TUNEL) test, cells were prepared as described (Madeo et al., 1997 (link)), and the DNA ends were labeled using the In Situ Cell Death Detection Kit, POD (Boehringer Mannheim). Yeast cells were fixed with 3.7% formaldehyde, digested with lyticase, and applied to a polylysine-coated slide as described for immunofluorescence (Adams and Pringle, 1984 (link)). The slides were rinsed with PBS and incubated with 0.3% H2O2 in methanol for 30 min at room temperature to block endogenous peroxidases. The slides were rinsed with PBS, incubated in permeabilization solution (0.1% Triton X-100 and 0.1% sodium citrate) for 2 min on ice, rinsed twice with PBS, incubated with 10 μl TUNEL reaction mixture (terminal deoxynucleotidyl transferase 200 U/ml, FITC-labeled dUTP 10 mM, 25 mM Tris-HCl, 200 mM sodium cacodylate, 5 mM cobalt chloride; Boehringer Mannheim) for 60 min at 37°C, and then rinsed 3× with PBS. For the detection of peroxidase, cells were incubated with 10 μl Converter-POD (anti-FITC antibody, Fab fragment from sheep, conjugated with horseradish peroxidase) for 30 min at 37°C, rinsed 3× with PBS, and then stained with DAB-substrate solution (Boehringer Mannheim) for 10 min at room temperature. A coverslip was mounted with a drop of Kaiser's glycerol gelatin (Merck). As staining intensity varies, only samples from the same slide were compared.
Free intracellular radicals were detected with dihydrorhodamine 123, dichlorodihydrofluorescein diacetate (dichlorofluorescin diacetate), or dihydroethidium (hydroethidine; Sigma Chemical Co.). Dihydrorhodamine 123 was added ad-5 μg per ml of cell culture from a 2.5-mg/ml stock solution in ethanol and cells were viewed without further processing through a rhodamine optical filter after a 2-h incubation. Dichlorodihydrofluorescein diacetate was added ad-10 μg per ml of cell culture from a 2.5 mg/ml stock solution in ethanol and cells were viewed through a fluorescein optical filter after a 2-h incubation. Dihydroethidium was added ad-5 μg per ml of cell culture from a 5 mg/ml aqueous stock solution and cells were viewed through a rhodamine optical filter after a 10-min incubation. For flow cytometric analysis, cells were incubated with dihydrorhodamine 123 for 2 h and analyzed using a FACS® Calibur (Becton Dickinson) at low flow rate with excitation and emission settings of 488 and 525–550 nm (filter FL1), respectively.
Free spin trap reagents N-tert-butyl-α−phenylnitrone (PBN; Sigma-Aldrich) and 3,3,5,5,-tetramethyl-pyrroline N-oxide (TMPO; Sigma-Aldrich) were added directly to the cell cultures as 10-mg/ml aqueous stock solutions. Viability was determined as the portion of cell growing to visible colonies within 3 d.
To determine frequencies of morphological phenotypes (TUNEL, Annexin V, DAPI, dihydrorhodamine 123), at least 300 cells of three independent experiments were evaluated.
Madeo F., Fröhlich E., Ligr M., Grey M., Sigrist S.J., Wolf D.H, & Fröhlich K.U. (1999). Oxygen Stress: A Regulator of Apoptosis in Yeast. The Journal of Cell Biology, 145(4), 757-767.
Publication 1999
3,3,5,5-tetramethyl-1-pyrroline N-oxide Annexin A5 Antibodies, Anti-Idiotypic Cacodylate Cardiac Arrest Cell Culture Techniques Cell Death Cells cobaltous chloride DAPI deoxyuridine triphosphate dichlorofluorescin dihydroethidium dihydrorhodamine 123 DNA Nucleotidylexotransferase Domestic Sheep Electron Microscopy Ethanol Flow Cytometry Fluorescein Fluorescein-5-isothiocyanate Formaldehyde Free Radicals Gelatins Glycerin Horseradish Peroxidase hydroethidine Immunofluorescence Immunoglobulins, Fab In Situ Nick-End Labeling lyticase Methanol Oxides Peroxidase Peroxidases Peroxide, Hydrogen Phenotype Polylysine Protoplasm pyrroline Rhodamine Sodium Sodium Citrate TERT protein, human Triton X-100 Tromethamine Yeast, Dried
4
Generating mKate2 Expression Under Xanf1 Promoter
Cited 30 times
To generate a construct expressing mKate2 under the control of Xanf1 promoter, the 2200 bp fragment of this promoter sufficient for specifically targeting the expression to the anterior neural plate [10 (link)] was subcloned into VspI (blunted)/AgeI sites of mKate2-N vector, instead of the multiple cloning site region and CMV (cytomegalovirus) promoter. For transgenic experiments, vectors were linearized by SfiI and purified using Qiagen columns. Transgenic embryos were generated by the REMI (restriction enzyme-mediated integration) technique exactly as described previously [10 (link)] and were analysed using a Leica MZFLIII fluorescent stereomicroscope with the rhodamine filter set: excitation filter 546/10 nm; suppression filter 565 nm. To visualize a fluorescent signal on tissue sections, transgenic embryos were fixed overnight in 4 % (w/v) formaldehyde in 0.1 % PBS, embedded in 3 % agarose in 0.1 % PBS and sectioned using a HB 650 Vibratome (Microme) in 50 μm sections. The animal work was performed in Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, in accordance with the regulations of the Department of Health and Human Services, National Institutes of Health.
SHCHERBO D., MURPHY C.S., ERMAKOVA G.V., SOLOVIEVA E.A., CHEPURNYKH T.V., SHCHEGLOV A.S., VERKHUSHA V.V., PLETNEV V.Z., HAZELWOOD K.L., ROCHE P.M., LUKYANOV S., ZARAISKY A.G., DAVIDSON M.W, & CHUDAKOV D.M. (2009). Far-red fluorescent tags for protein imaging in living tissues. The Biochemical journal, 418(3), 567-574.
Publication 2009
Animals Animals, Transgenic Cloning Vectors Cytomegalovirus DNA Restriction Enzymes Embryo Formaldehyde Neural Plate Rhodamine Sepharose Tissues
5
TUNEL Staining Protocol for Planarians
Cited 19 times

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Publication 2009
Acetylcysteine Amputation Animals Antibodies, Anti-Idiotypic Buffers Cloning Vectors Deoxyribonuclease I Digoxigenin DNA Breaks, Single-Stranded Enzymes Formaldehyde Immunoglobulins In Situ Nick-End Labeling Mucus Peroxide, Hydrogen Planarians Radiotherapy Rhodamine Transferase Triton X-100

Most recents protocols related to «Rhodamine»

1
Synthesis of Rhodamine Formamidine
Not available on PMC !

Example 121

[Figure (not displayed)]

Rhodamine formamidine 175. 5-Aminorhodamine 174 (0.100 g, 0.249 mmol), (chloromethylene)dimethylimminium chloride (0.304 g, 2.49 mmol), and DIEA (0.50 mL, 2.87 mmol) were stirred in 4 mL of DMF for 16 h. The reaction mixture was evaporated and the crude product was purified on a silica gel column (1.5×25 cm bed, patched in MeCN/H2O/AcOH (8:2:2.5)), eluant: MeCN/H2O/AcOH (8:2:2.5) to give amidine 175 (0.050 g, 44%) as a red solid.

US11867698B2. Fluorogenic pH sensitive dyes and their method of use (2024-01-09). Life Technologies Corporation [US]. Inventors: Jeffrey Dzubay [US], Kyle Gee [US], Vladimir Martin [US], Aleksey Rukavishnikov [US], Daniel Beacham [US].
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Patent 2024
Amidines Anabolism Chlorides formamidine N,N-diisopropylethylamine Rhodamine Silica Gel tetramethylrhodamine
2
Synthesis of X-Phodamine pH-Sensor 162
Not available on PMC !

Example 116

[Figure (not displayed)]

X-Phodamine PH-sensor 162. Aldehyde 159 (0.336 g, 1.0 mmol), 8-hydroxyjullolidine (0.416 g, 2.2 mmol), and 10-camphorsulfonic acid (20 mg, catalyst) were stirred for 18 h in propionic acid (10 mL) at 65-70° C., cooled to rt and poured into aqueous 3N NaOAc (200 mL) and sat. NaHCO3 (10 mL). The mixture was extracted with CHCl3 (7×40 mL), extract was washed with brine (200 mL), filtered through paper filter, and evaporated to give a crude dihydro derivative 161. A sample of this compound (40 mg, 0.06 mmol) was vigorously stirred in CHCl3 (50 mL) with Bengal Rose (10 mg, catalyst) in an open beaker under the sunlamp illumination for 18 h. After evaporation the residue was purified by preparative TLC on two silica gel plates using 7% H2O in MeCN as eluant to give compound 162 (11 mg, 24%) as a dark red solid.

US11867698B2. Fluorogenic pH sensitive dyes and their method of use (2024-01-09). Life Technologies Corporation [US]. Inventors: Jeffrey Dzubay [US], Kyle Gee [US], Vladimir Martin [US], Aleksey Rukavishnikov [US], Daniel Beacham [US].
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Patent 2024
10-camphorsulfonic acid 11-dehydrocorticosterone Aldehydes Anabolism Bicarbonate, Sodium brine Chloroform Lighting propionic acid Rhodamine Rose Bengal Silica Gel
3
Antibody Protocols for Westerns and IF
Details about antibodies used for Western blotting and immunofluorescence experiments are in Table S1. Fluorophore-conjugated or horseradish peroxidase secondary antibodies were from Jackson ImmunoResearch. Rhodamine-EGF was generated in-house as previously described by (73 (link)).
Rahmani S., Ahmed H., Ibazebo O., Fussner-Dupas E., Wakarchuk W.W, & Antonescu C.N. (2023). O-GlcNAc transferase modulates the cellular endocytosis machinery by controlling the formation of clathrin-coated pits. The Journal of Biological Chemistry, 299(3), 102963.
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Publication 2023
Antibodies Fluorescent Antibody Technique Horseradish Peroxidase Rhodamine
4
Endocytic Processes in ARPE-19 Cells
ARPE-19 cells stably expressing eGFP-CLC were seeded onto glass coverslips the day before the experiment, and in some cases gone through siRNA transfection (Figure 1, Figure 2, Figure 3, Figure 5, Figure 6, Figure 7). pSBTet-AAK1-GFP WT and pSBTet-AAK1-GFP mutant cells were seeded onto glass coverslips the day before the experiment for siRNA of AAK1 in Figure 3. After two rounds of siRNA transfection to AAK1, doxycycline was added to the cells during the rest day. Cells were incubated for 4h at 20 μM TMG for or in vehicle (dH20) prior to live-cell microscopy (Fig. 2). Cells were either incubated for 3h at 5 μM of LP-935509 or in vehicle control (0.1% (v/v) DMSO) prior to the fixation (Fig. 6). Following 1-h serum starvation, cells were treated with 20 ng/ml rhodamine EGF and 10 μg/ml A657-Tfn in combination (Fig. 3), followed by immediate fixation in 4% paraformaldehyde. For Figure 7, cells were seeded onto 6-well plates for 24 prior to incubation in media without glucose, glutamine, and no phenol red (catalog no. A1443001) supplemented with 10% dialyzed fetal bovine serum (Thermo Fisher Scientific), and glucose and/or glutamine, as indicated, for 4 h.
Rahmani S., Ahmed H., Ibazebo O., Fussner-Dupas E., Wakarchuk W.W, & Antonescu C.N. (2023). O-GlcNAc transferase modulates the cellular endocytosis machinery by controlling the formation of clathrin-coated pits. The Journal of Biological Chemistry, 299(3), 102963.
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Publication 2023
Cells Doxycycline Fetal Bovine Serum Glucose Glutamine Microscopy paraform Rhodamine RNA, Small Interfering Serum Sulfoxide, Dimethyl Transfection
5
In vitro Virus-Sperm Binding Assay
To detect the binding of RdFV CP with sperms in vitro, His-tag-fused CP was expressed in Escherichia coli strain Rosetta, and the proteins were purified using nickel-nitrilotriacetic acid resin (Qiagen). Sperm smears collected from testes of RdFV-free R. dorsalis were successively incubated with the purified CP (0.5 μg/μl) for 1 h, smeared on poly-lysine-treated glass slides, immunolabeled with CP-rhodamine (0.5 μg/μl), stained with DAPI (2.0 μg/ml), and then processed for immunofluorescence microscopy.
In neutralization experiments to test the direct interaction between RdFV CP and HongrES1, mature sperms excised from the testes of RdFV-free R. dorsalis were pre-incubated for 30 min with pre-immune antibody (0.5 μg/μl) or HongrES1 antibody (0.5 μg/μl), and then the in vitro CP-sperm binding experiment was performed as described above.
In neutralization experiments to test the direct interaction between RGDV particles and HongrES1, mature sperms excised from RGDV-free leafhoppers were pre-incubated for 30 min with pre-immune antibody (0.5 μg/μl) or HongrES1 antibody (0.5 μg/μl), incubated with the purified RGDV particles (1.0 μg/μl) for 1 h, smeared on poly-lysine-treated glass slides, immunolabeled with P8-FITC (0.5 μg/μl), stained with DAPI (2.0 μg/ml), and then processed for immunofluorescence microscopy.
Wan J., Liang Q., Zhang R., Cheng Y., Wang X., Wang H., Zhang J., Jia D., Du Y., Zheng W., Tang D., Wei T, & Chen Q. (2023). Arboviruses and symbiotic viruses cooperatively hijack insect sperm-specific proteins for paternal transmission. Nature Communications, 14, 1289.
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Publication 2023
DAPI Escherichia coli Fluorescein-5-isothiocyanate Immunofluorescence Microscopy Immunoglobulins Leafhoppers Lysine nickel nitrilotriacetic acid Poly A Proteins Resins, Plant Rhodamine Sperm Sperm Maturation Strains Testis

Top products related to «Rhodamine»

1
4 6 diamidino 2 phenylindole (dapi) by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore
DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
2
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Italy, Australia, France, Switzerland, Spain, Belgium, Denmark, Panama, Poland, Singapore, Austria, Morocco, Netherlands, Sweden, Argentina, India, Finland, Pakistan, Cameroon, New Zealand
DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
3
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, France, Canada, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
4
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
5
Alexa fluor 488 by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Japan, France, Italy, Canada, China, Spain, Switzerland, Denmark, Australia, Hungary, Belgium, Ireland, Israel, Netherlands, Moldova, Republic of, India, Austria, Czechia, Poland
Alexa Fluor 488 is a fluorescent dye used in various biotechnological applications. It has an excitation maximum at 495 nm and an emission maximum at 519 nm, producing a green fluorescent signal. Alexa Fluor 488 is known for its brightness, photostability, and pH-insensitivity, making it a popular choice for labeling biomolecules in biological research.
6
Vectashield by Vector Laboratories
Sourced in United States, United Kingdom, Germany, Canada, Japan, France, Switzerland, Panama, Spain, China, Italy
Vectashield is a non-hardening, aqueous-based mounting medium designed for use with fluorescent-labeled specimens. It is formulated to retard photobleaching of fluorescent dyes and provides excellent preservation of fluorescent signals.
7
Lsm 710 by Zeiss
Sourced in Germany, United States, United Kingdom, Japan, Switzerland, France, China, Canada, Italy, Spain, Singapore, Austria, Hungary, Australia
The LSM 710 is a laser scanning microscope developed by Zeiss. It is designed for high-resolution imaging and analysis of biological and materials samples. The LSM 710 utilizes a laser excitation source and a scanning system to capture detailed images of specimens at the microscopic level. The specific capabilities and technical details of the LSM 710 are not provided in this response to maintain an unbiased and factual approach.
8
4 6 diamidino 2 phenylindole (dapi) by Vector Laboratories
Sourced in United States, United Kingdom, Canada, Germany, Japan, China, Spain, France, Colombia
DAPI is a fluorescent dye used for staining and visualizing DNA in biological samples. It binds to the minor groove of DNA, emitting blue fluorescence when excited by ultraviolet or violet light. DAPI is commonly used in fluorescence microscopy and flow cytometry applications.
9
Rhodamine red x by Jackson ImmunoResearch
Sourced in United States, Panama
Rhodamine Red-X is a fluorescent dye used in various biological applications. It has an excitation wavelength of approximately 570 nm and an emission wavelength of approximately 590 nm, making it suitable for detection and labeling purposes.

More about "Rhodamine"

Rhodamine is a versatile group of fluorescent dyes commonly used in biochemical and biomedical research.
These dyes, also known as Rhodamine B, Rhodamine 6G, and Rhodamine 123, exhibit bright, photostable fluorescence that makes them indispensable tools for researchers.
Rhodamine dyes can be conjugated to a variety of biomolecules, including antibodies, nucleic acids, and proteins, enabling the visualization and tracking of cellular processes.
One of the key applications of Rhodamine dyes is in flow cytometry, where they are used as labels to identify and sort cells based on their fluorescent properties.
In microscopy, Rhodamine dyes are employed as tracers and probes to study cellular structures and dynamics.
They are often used in combination with other fluorescent dyes, such as DAPI (4',6-diamidino-2-phenylindole) for nuclear staining and Alexa Fluor 488 for protein labeling.
To enhance the performance of Rhodamine-based experiments, researchers may use detergents like Triton X-100 to permeabilize cell membranes and Bovine Serum Albumin (BSA) to block non-specific binding.
Mounting media like Vectashield can also be used to preserve the fluorescence of Rhodamine-labeled samples.
The versatility of Rhodamine dyes extends to their use in a variety of imaging techniques, from confocal microscopy (e.g., LSM 710) to super-resolution microscopy.
Rhodamine Red-X, a specific variant of the Rhodamine family, is frequently used in fluorescence-based protein detection and quantification assays.
Overall, Rhodamine dyes are essential tools in the life sciences, enabling researchers to visualize, track, and study cellular processes with high sensitivity and precision.
Their unique optical properties and ability to be conjugated to biomolecules make them indispensable for a wide range of applications in the field of biochemistry and biology.