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Rosmarinic acid

Rosmarinic acid is a natural compound found in various plants, including rosemary, sage, and mint.
It has been studied for its potential health benefits, such as antioxidant, anti-inflammatory, and neuroprotective properties.
Researchers use PubCompare.ai to optimize their Rosmarinic acid research by locating the most effective protocols from literature, preprints, and patents.
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Most cited protocols related to «Rosmarinic acid»

1
Western Blot Protein Expression Analysis
Western blot was performed referring to our previous articles 24 (link)-26 (link). In brief, proteins from cultured cells or homogenized left ventricles were separated by 10% SDS-PAGE and were electrophoretically transferred onto PVDF membranes (EMD Millipore, Billerica, MA, USA; No. IPFL00010). Membranes were blocked with 5% nonfat milk for 60min at room temperature, and were subsequently probed with indicating primary antibodies overnight at 4°C, followed with the secondary antibodies at 37°C for 1 hour. The bands were scanned and quantified by Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA), and protein expressions were normalized to total proteins or GAPDH. Nuclear and cytosolic protein fractions were separated by a commercial kit as our previously described 9 (link), 21 (link). Proteins from cytosolic lysates were normalized to GAPDH, whereas proteins from nuclear lysates were normalized to PCNA.
Total RNA was isolated using TRIzol according to our previous studies and reverse transcribed with Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, MA, USA)27 (link). The expression level of each individual transcript was normalized to Gapdh.
Zhang X., Zhu J.X., Ma Z.G., Wu H.M., Xu S.C., Song P., Kong C.Y., Yuan Y.P., Deng W, & Tang Q.Z. (2019). Rosmarinic acid alleviates cardiomyocyte apoptosis via cardiac fibroblast in doxorubicin-induced cardiotoxicity. International Journal of Biological Sciences, 15(3), 556-567.
Publication 2019
Anabolism Antibodies Cultured Cells Cytosol DNA, Complementary GAPDH protein, human Left Ventricles Milk, Cow's Nuclear Proteins polyvinylidene fluoride Proliferating Cell Nuclear Antigen Proteins SDS-PAGE Tissue, Membrane trizol Western Blotting
2
Protein and mRNA quantification in cardiac cells
After being extracted from the frozen pulverized left ventricles or cultured cells and quantified, total proteins were prepared for western blot analysis and normalized to the matched total proteins or GAPDH according to our previous study52 (link). Briefly, separated proteins were incubated with the indicated primary antibodies overnight at 4 °C and with secondary antibodies for 60 min at room temperature. Nuclear and cytosolic protein fractions were separated using a commercial kit (Thermo Fisher Scientific) according to the manufacturer′s protocol. Proteins from cytosolic lysates were normalized to GAPDH, whereas proteins from nuclear lysates were normalized to PCNA.
Isolated total mRNA from hearts and cultured cells was reversely transcribed to complementary DNA (cDNA) with Transcriptor First Strand cDNA Synthesis Kit (Roche (Basel, Switzerland), 04896866001). Transcriptional level of target genes were normalized to Gapdh, and the primers for quantitative real-time PCR are shown in Supplementary Table 3.
Zhang X., Ma Z.G., Yuan Y.P., Xu S.C., Wei W.Y., Song P., Kong C.Y., Deng W, & Tang Q.Z. (2018). Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling. Cell Death & Disease, 9(2), 102.
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Publication 2018
Anabolism Antibodies Cultured Cells Cytosol DNA, Complementary Freezing GAPDH protein, human Heart Left Ventricles Nuclear Proteins Oligonucleotide Primers Proliferating Cell Nuclear Antigen Proteins Real-Time Polymerase Chain Reaction RNA, Messenger Transcription, Genetic Western Blot
3
Echocardiography and Hemodynamic Monitoring in Mice
Transthoracic echocardiography was performed by a MyLab 30CV ultrasound (Esaote SpA, Genoa, Italy) with a 10 MHz phased array transducer as previously described56 (link),57 (link). Light anesthetized by 1.5% isoflurane, mice were lightly secured in a warming pad with a shallow left lateral position after the precordium shaved. After imaged with two-dimensional (2D) mode in the parasternal long-axis and/or parasternal short-axis at the level close to papillary muscles, a 2D guided M-mode trace crossing the anterior and posterior wall of the left ventricle was recorded and the morphological parameters of the heart were collected and calculated. Attention was given not to bring excessive pressure to the chest, which could cause bradycardia and deformation of the heart.
Invasive hemodynamic monitoring was performed by cardiac catheterization according to our previous articles56 (link),57 (link). In brief, mice was placed on a warmed surgical platform with supine position and the right carotid artery was isolated, exposed. Then a 1.4-French Millar catheter transducer (SPR-839; Millar Instruments, Houston, TX) was inserted into the left ventricle through the isolated carotid artery. And data were analyzed using the PVAN data analysis software.
Zhang X., Ma Z.G., Yuan Y.P., Xu S.C., Wei W.Y., Song P., Kong C.Y., Deng W, & Tang Q.Z. (2018). Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling. Cell Death & Disease, 9(2), 102.
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Publication 2018
Attention Catheterizations, Cardiac Catheters Chest Common Carotid Artery Echocardiography Epistropheus Heart Isoflurane Left Ventricles Light Mice, House Operative Surgical Procedures Papillary Muscles Pressure Transducers Ultrasonography
4
Microgreen Phenotyping and Phytochemical Analysis
Total cotyledon area was measured using a Scanalyzer PL (LemnaTec, Aachen, Germany), which allowed photographing the boxes containing microgreens from a fixed height and calculations of areas. Measurements were performed over an 8 days period starting with day 6 after sowing, up to the point where leaves from different individuals began to overlap, preventing accurate measurements. Boxes were photographed sequentially, minimizing the time spent outside the defined light treatment, generally 1 min. The software setup for the phenotyping system was similar to that previously described by Arvidsson et al. [68 (link)].
Chlorophyll and carotenoid pigments were extracted in 80% aqueous acetone and quantified by spectrophotometric means using formulas described in Wellburn [69 (link)]. Chlorophyll fluorescence was measured using a FMS2 fluorometer (HansaTech, Norfolk, UK) for 10 cotyledons/treatment. Chlorophyll related analyses were performed at the end of the experiment, at 17 days after sowing.
For total phenolics, flavonoids, free radical scavenging activity and anthocyanin evaluations, 10 plantlets were pooled from each of 2 boxes per treatment at defined days, resulting a total of 20 plantlets/treatment/day of assessment. Total phenolics, flavonoids and free radical scavenging activity were assayed in 70% ethanolic extracts, obtained by maceration for 24 h at room temperature, according to methods described in Herald et al. [70 (link)]. Anthocyanin determinations were performed according to Lee et al. [71 (link)] in methanol:water:hydrochloric acid (70:29:1) mixture extracts. For each assessment, 3 technical replicates were performed.
Individual phenolic acids at 17 days after sowing were determined using a Shimadzu High Performance liquid chromatography (HPLC) (Shimadzu LC-10ADVP, Columbia, MD, USA) coupled to a photodiode array (PDA) detector (Shimadzu SPD-M20A, Columbia, MD, USA). The chromatographic conditions were represented by a Macherey-Nagel C18 reverse phase (150 mm × 4.6 mm × 4 µm) column, water:acetic acid (99:1) (A) and methanol (B) mobile phases, 0.6 mL/min flow rate and 40 °C. The chromatographic program was 0 min 100% A, 5 min 6% B, 5–7 min 6% B, 50 min 30% B, 50–52 min 30% B, 62 min 100% B. Compounds (rosmarinic and caffeic acids) were quantified using external standards, HPLC grade (Sigma, Steinheim, Germany).
Lobiuc A., Vasilache V., Pintilie O., Stoleru T., Burducea M., Oroian M, & Zamfirache M.M. (2017). Blue and Red LED Illumination Improves Growth and Bioactive Compounds Contents in Acyanic and Cyanic Ocimum basilicum L. Microgreens. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(12), 2111.
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Publication 2017
Acetic Acid Acetone Anthocyanins AT 17 Caffeic Acids Carotenoids Chlorophyll Chromatography Cotyledon Diet, Formula Ethanol Flavonoids Fluorescence Free Radicals High-Performance Liquid Chromatographies Hydrochloric acid hydroxybenzoic acid Light Methanol Pigmentation Spectrophotometry
5
Colitis Alleviation by 5-ASA and Rosmarinic Acid
Experimental colitis was induced by giving mice drinking water ad libitum containing 5% (w/v) DSS for 7 days. Mice of each of the groups were monitored carefully every day to confirm that they had consumed an approximately equal volume of water containing DSS. For each experiment, the mice were divided into five experimental groups (n = 10/group). The first group was kept as the vehicle-treated control, and the second group was given drinking water with DSS only during the experimental period. The other three groups consisted of mice receiving 5% DSS who were administrated 5-ASA (75 mg/kg/day p.o.) or rosmarinic acid (30 or 60 mg/kg/day p.o.) daily for 7 days, according to the experimental design. All materials were dissolved in a vehicle of 0.9% saline. Control groups were given the vehicle daily for 7 days as appropriate. Administration of each drug was initiated simultaneously with the DSS treatment.
Jin B.R., Chung K.S., Cheon S.Y., Lee M., Hwang S., Noh Hwang S., Rhee K.J, & An H.J. (2017). Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of NF-κB and STAT3 activation. Scientific Reports, 7, 46252.
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Publication 2017
Colitis Mesalamine Mice, House Normal Saline rosmarinic acid

Most recents protocols related to «Rosmarinic acid»

1
Extraction and Quantification of Rosmarinic Acid
Not available on PMC !
The extraction technique for rosmarinic acid (RA) followed the guidelines provided by Komali and Kalidas [47] (link) and Lopez-Arnaldos et al. [48] (link). For the RA extraction, 200 mg of leaf tissue was homogenized in 10 ml of 50% methanol using a Bio-Homogenizer M 133/128-0. The mixture was heated to 55°C for two hours and centrifuged for ten minutes at 3,500 rpm. Using a Unico W49376 Spectrophotometer 1200 (Shanghai, China), the absorbance at 333 nm was measured after one ml of the extract was diluted with nine ml of 50% methanol, after which the content of rosmarinic acid was determined.
El-Naggar H.M, & Osman A.R. (2024). Enhancing growth and bioactive metabolites characteristics in Mentha pulegium L. via silicon nanoparticles during in vitro drought stress. BMC plant biology, 24(1).
Publication 2024
2
Quantification of Rosmarinic Acid in Plant Extracts
The rosmarinic acid content was assessed in extracts prepared from 100 mg of DW (dry weight) tissue in 2 mL of HPLC-grade methanol. Tissue was extracted by sonication (2 × 30 min at 23 ± 2 °C) (ultrasonic bath, Polsonic, Warsaw, Poland). Extracts were centrifuged (25,255×g for 8 min, 4 °C), with samples filtered through Millex®GP 0.22-μm syringe filters (Millipore, Merck, Darmstadt, Germany) for analysis by diode array detector high-pressure liquid chromatography (DAD-HPLC). The validated method was applied for quantitative analyses of rosmarinic acid using Merck-Hitachi (LaChrom Elite, Tokyo, Japan) apparatus and on a RP-18 column (Purospher, 5 μm, 250 × 4 mm; Merck, Darmstadt, Germany) (Sułkowska-Ziaja et al. 2016 (link)). The parameters were as follows: flow rate 1 mL/min, A—methanol, 0.5% acetic acid 1:4, B—methanol (v/v), temp. 25 °C, injection 10 µL, λ = 254 nm. The gradient program was as follows: 0 to 20 min, 0% B; 20 to 35 min, 0–20% B; 35 to 45 min, 20–30% B; 45 to 55 min, 30–40% B; 55 to 60 min, 40–50% B; 60 to 65 min, 50–75% B; and 65 to 70 min, 75–100% B. Identification was done by comparison to Rt (retention time) and UV spectra of reference substance of rosmarinic acid (Sigma-Aldrich Co, Merck, Darmstadt, Germany). Assays were done in three replications, with results expressed in mg/100 g DW ± SD.
Motyka S., Szopa A, & Ochatt S.J. (2024). Distinction of chia varieties in vivo and in vitro based on the flow cytometry and rosmarinic acid production. Applied Microbiology and Biotechnology, 108(1), 337.
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Publication 2024
3
Comprehensive Rosmarinic Acid Target Mapping
The keyword “Rosmarinic acid” was used to retrieve relevant genes from six databases: TCMSPS (https://old.tcmsp-e.com/tcmsp.php), SwissTargetPrediction (http://www.swisstargetprediction.ch/), Drugbank (https://go.drugbank.com/), Binding database (http://www.bindingdb.org/bind/index.jsp), STITCH (http://stitch.embl.de/), and Therapeutic Target Database (http://db.idrblab.net/ttd/). The targets from these six databases were combined, and the repeat duplicate values were removed to obtain the comprehensive set of ROSA-related targets.
Liao X., Xin J., Yu Z., Yan W., Li C., Cao L., Zhang H, & Wang W. (2024). Unlocking the antiviral potential of rosmarinic acid against chikungunya virus via IL-17 signaling pathway. Frontiers in Cellular and Infection Microbiology, 14, 1396279.
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Publication 2024
4
HPLC Analysis of O. aristatus Extracts
Not available on PMC !
The HPLC method used to measure the ethyl acetate extracts of O. aristatus genotypes was adapted from Batubara et al. (2020) (link) with some modifications. Separate standard solutions of rosmarinic acid and sinensetin (ChromaDex) were prepared in methanol at concentrations ranging from 1-100 μg/mL and 0.3-24 μg/mL, respectively. The analysis was conducted by an HPLC system (Shimadzu, Kyoto, Japan) that was equipped with a degassing unit (DGU-20A5R), pump (LC-20AB), autosampler (SIL-20A HT), column oven (CTO-20AC), and UV-Vis detector (SPD-20A). The HPLC system was used with a Shim-Pack VP ODS C18 Shimadzu column (75×4.6 mm i.d., 3 μm pore size).
The mobile phases used for elution were an aqueous solution containing 0.1% formic acid (A) and acetonitrile containing 0.1% formic acid (B). The gradient elution system was as follows: from 0-7 minutes, the percentage of B increased from 2% to 20%; from 7-10 minutes, the percentage of B increased from 20% to 30%; from 10-18 minutes, the percentage of B increased from 30% to 50%; from 18-20.5 minutes, the percentage of B increased from 50% to 98%; from 20.5-23 minutes, the percentage of B remained at 98%; and from 23-26 minutes, the percentage of B decreased from 98% to 2%. The system was stabilized for 35 minutes before the next injection. For each genotype sample, 10 µL of injection volume was used, and the mobile phase flow rate was set at 1 mL/min, with a detector wavelength of 320 nm and a temperature of 30°C for 35 minutes. The rosmarinic acid and sinensetin concentrations were expressed in mg/g DW based on their respective calibration curves for standards.
BOVANI R.P., Liwanda N., Batubara I., Ambarsari L., & NURCHOLIS W. (2024). Phytochemical content and antioxidant capacity of ethyl acetate extracts from fifteen Orthosiphon aristatus leaves genotypes. Biodiversitas Journal of Biological Diversity, 25(2).
Publication 2024
5
Quantification of Rosmarinic Acid in Dried Leaves
Not available on PMC !
Extraction was done in 1 mL of 80% methanol from the 20 mg dried leaf samples. This was successively followed by vortexing and sonication up to 30 min. Finally, the centrifugation of the extract was done at 10,000 rpm for 15 min. Identification and quantification of rosmarinic acid in HPLC was done following the published method of Dey et al. (2023) (link) with slight modification in elution gradient. The mobile phase consisted of A (0.1% TFA in water) and B (acetonitrile). The protocol for elution was 0-2 min 10% B, 2-5 min 15% B, 5-10 min hold 15% B, 10-15 min 22% B, 15-45 min 65% B, 45-50 min 90% B with a flow rate of 1 mL min -1 and injection volume was 50 μL.
Dey P.K., Lakhanpal M., & Mitra A. (2024). Metabolite profiling of sugar and phenolics provide clues for postharvest anthocyanin accumulation in detached leaves of lemon balm for possible utilization in food products. Emirates Journal of Food and Agriculture, 36, 1-10.
Publication 2024

Top products related to «Rosmarinic acid»

1
Rosmarinic acid by Merck Group
Sourced in United States, Germany, Italy, Poland, France, Spain, Portugal, Sao Tome and Principe, United Kingdom, Brazil, Ireland, Australia, China
Rosmarinic acid is a natural polyphenolic compound found in various plant species, including rosemary, sage, and lemon balm. It is a commonly used laboratory reagent for research purposes.
2
Caffeic acid by Merck Group
Sourced in United States, Germany, Italy, France, Poland, Spain, China, United Kingdom, Australia, Sao Tome and Principe, Switzerland, India, Ireland, Canada, Macao, Brazil, Austria, Mexico, Czechia, Portugal
Caffeic acid is a phenolic compound commonly found in various plants. It serves as a laboratory standard for the identification and quantification of similar phenolic compounds using analytical techniques such as high-performance liquid chromatography (HPLC) and spectrophotometry.
3
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
4
Quercetin by Merck Group
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Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
5
Chlorogenic acid by Merck Group
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Chlorogenic acid is a compound found in various plants, including coffee beans. It is a type of polyphenol and is commonly used in laboratory settings for research purposes.
6
Rutin by Merck Group
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Rutin is a laboratory reagent used for analytical and research purposes. It is a flavonoid compound derived from various plant sources. Rutin exhibits antioxidant and anti-inflammatory properties, and is commonly used in assays, chromatography, and other analytical techniques.
7
Luteolin by Merck Group
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Luteolin is a laboratory equipment product manufactured by Merck Group. It is a flavonoid compound used as a chemical standard and reference material for analytical and research applications.
8
P coumaric acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, China, Poland, United Kingdom, Sao Tome and Principe, Switzerland, Canada, Ireland, India, Australia, Japan, Macao, Portugal
P-coumaric acid is a naturally occurring phenolic compound that can be utilized as a reference standard or an analytical reagent in various laboratory settings. It is a white to off-white crystalline solid that is soluble in organic solvents. P-coumaric acid is commonly used as a standard in analytical techniques, such as high-performance liquid chromatography (HPLC) and spectrophotometric measurements, to quantify and characterize similar compounds in sample matrices.
9
Apigenin by Merck Group
Sourced in United States, Germany, Italy, Poland, France, India, Spain, Sao Tome and Principe, China, Switzerland, Macao, Ireland, Portugal, Austria
Apigenin is a naturally occurring plant flavonoid compound. It is a light yellow crystalline solid that is widely used as a laboratory reagent in biochemical research.
10
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

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