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Ryanodine

Ryanodine is a complex organic compound derived from the alkaloid ryanodine, which is extracted from the roots of certain plant species.
It acts as a specific agonist of the ryanodine receptor, a calcium release channel found in the sarcoplasmic reticulum of muscle cells.
Ryanodine binds to and modulates the activity of this receptor, playing a key role in the regulation of intracellular calcium homeostasis and excitation-contraction coupling in cardiac and skelatal musle.
Ryanodine is a valuable tool for the study of calcium signaling and muscle fuction, and has important implications for the understanding and treatment of various muscular and cardiovascular disoders.
Experince the power of AI-driven research optimization today with PubCompare.ai's protocol comparison tool for improved reproducibility in your Ryanodine studies.

Most cited protocols related to «Ryanodine»

1
Hyperalgesic Priming Induction Protocol
Cited 7 times
The procedure to induce hyperalgesic priming was based on a previously described protocol12 (link)20 (link). The inducer of priming (ryanodine or PPT, in this study) was injected intradermally on the dorsum of the hind paw, at the site of nociceptive testing. The presence of priming was confirmed, 3–5 days later, depending on the experiment, by the injection of PGE2 (100 ng), at the same site. The mechanical hyperalgesia induced by injection of PGE2 in the previously untreated naïve control paw lasts no more than 2 h57 (link), and in this study is represented by the reduction in the mechanical threshold evaluated 30 min after PGE2 injection, which expresses its acute hyperalgesic effect, even in the previously primed paw11 (link)12 (link); The prolongation of PGE2 hyperalgesia to greater than 4 h is used as a marker for the presence of priming7 (link)10 (link)12 (link)58 (link), produced by activation of an additional signaling pathway9 (link)11 (link)12 (link), and represented as the reduced mechanical threshold at the 4th h time point12 (link). Importantly, immediately before the tests for priming with PGE2, the mechanical nociceptive threshold is not significantly different from the mechanical baseline threshold evaluated before the injection of the priming inducer (3–5 days previously, see Supplementary Table S1).
Ferrari L.F., Khomula E.V., Araldi D, & Levine J.D. (2016). Marked Sexual Dimorphism in the Role of the Ryanodine Receptor in a Model of Pain Chronification in the Rat. Scientific Reports, 6, 31221.
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Publication 2016
Dinoprostone Hyperalgesia Mechanical Allodynia Ryanodine
2
Multi-State Cryo-EM Structural Analysis of RyR1
Cited 7 times

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Publication 2016
Caffeine Complement Factor B Cryoelectron Microscopy Egtazic Acid Helix (Snails) Human Body Ligands Microtubule-Associated Proteins Muscle Rigidity Python Reconstructive Surgical Procedures Ryanodine Ryanodine Receptor 1 Tandem Repeat Sequences
3
Structural Insights into InsP3 Receptor
Cited 7 times
The N-terminal (NT, residues 1-604) of rat InsP3R1 in which all Cys were replaced by Ala (NTCysless) was expressed in E. coli and purified. Crystals of NTCysless were grown by the hanging-drop vapour diffusion method in 0.1 M Hepes pH 7.0, 0.8-1.0 M (NH4)2SO4, and 3% (v/v) trimethylamine N-oxide for apo-state crystals, or 0.1 M Na citrate (pH 6.0), 8% (w/v) PEG-6000, 70 mM Li2SO4, 3% dimethyl sulfoxide for InsP3-bound crystals. Diffraction data were collected at 100 K on the 19-ID (apo-crystals) or 19-BM (InsP3-bound crystals) beam lines at the Advanced Photon Source Synchrotron facility (Argonne, IL) and processed with HKL200028 . Structures of apo-NTCysless at 3.0 Å resolution and InsP3-bound NTCysless at 3.6 Å resolution were determined by molecular replacement using structures of the SD (PDB code: 1XZZ)3 (link) and the IBC (1N4K)2 (link) as search models with the program Phaser29 (link). Iterative refinement and model building were performed with Refmac5 and Coot, respectively. Numbering of secondary structure motifs is in accord with Supplementary Figure 7. Binding of 3H-InsP3 or 3H-ryanodine to full-length InsP3R1, chimeras of InsP3R1 and RyR, and to related N-terminal fragments was defined using equilibrium-competition binding assays4 (link). Functional properties of InsP3R1 and chimeras were characterized after stable expression in DT40 cells lacking endogenous InsP3R4 (link). A luminal Ca2+ indicator was used to record InsP3-evoked Ca2+ release from the intracellular stores of permeabilized DT40 cells4 (link).
Seo M.D., Velamakanni S., Ishiyama N., Stathopulos P.B., Rossi A.M., Khan S.A., Dale P., Li C., Ames J.B., Ikura M, & Taylor C.W. (2012). Structural and functional conservation of key domains in InsP3 and ryanodine receptors. Nature, 483(7387), 108-112.
Publication 2011
Cells Chimera Citrate Diffusion Escherichia coli HEPES Inositol 1,4,5-Trisphosphate ITPR1 protein, human Phenobarbital Polyethylene Glycol 6000 Protoplasm Ryanodine Sulfoxide, Dimethyl trimethyloxamine
4
Optical Mapping of Ventricular Arrhythmias
Cited 7 times
An expanded Methods section is available in the online Data Supplement.
Hearts of New Zealand White rabbits (n=45) were perfused with 37°C oxygenated normal Tyrode's solution. We simultaneously mapped Cai and Vm using Rhod-2 AM and RH237 on epicardial (n=14) or endocardial (n=14) surface of ventricles. In additional hearts, we mapped epicardial surfaces to explore the effect of IK1 suppression (n=8), and endocardial surfaces to study the effect of ryanodine (n=3) and IKr blocker, E-4031(n=2). Transmembrane potential (TMP) was recorded in an additional 4 rabbits. Cytochalasin D (10 μmol/L, n=7) or blebbistatin (10 to 20 μmol/L, n=15) or both (n=23) was used to inhibit contraction during optical and TMP recordings (online Supplement Figure I).
Atrioventricular block was created in all hearts with cryoablation. VF was induced with burst ventricular pacing, and defibrillated with transvenous electrodes. To characterize spontaneous Cai elevation (SCaE), we performed ventricular pacing for 100 beats at a pacing cycle length (PCL) of 600, 500, 400, 300, 200 ms, and the minimum cycle length with 1:1 pacing capture. When substantial SCaEs emerged, pacing at the same PCL with different numbers of paced beats (50, 200, 300, and 400) were performed (n=6) to study a dependence of SCaE on the pacing duration. Isoproterenol at various concentrations (0.01 to 1.0 μmol/L) was administered, and defibrillation and pacing protocols were repeated. We defined the amplitude of baseline Vm and Cai transient as 1 arbitrary unit (AU). A focal ectopy with DAD at the earliest activation site (the onset of the optical action potential was required to precede the QRS onset of pseudo-ECG if it was available) was defined as TA.
Mitsunori M., Boyoung J., Liang T., Tetsuji S., Young-Keun O., Seongwook H., Eue-Keun C., Dae-Hyeok K., Mark J. S., James N. W., Shien-Fong L, & Peng-Sheng C. (2009). Diastolic Intracellular Calcium-Membrane Voltage Coupling Gain and Postshock Arrhythmias: Role of Purkinje Fibers and Triggered Activity. Circulation research, 106(2), 399.
Publication 2009
Action Potentials Atrioventricular Block blebbistatin Cardiac Arrest Cryosurgery Cytochalasin D Dietary Supplements E 4031 Ectopic Tissue Electric Countershock Endocardium Heart Heart Ventricle Isoproterenol Membrane Potentials New Zealand Rabbits Oryctolagus cuniculus PACE protocol rhod-2 Ryanodine Transients Tyrode's solution Vision
5
Estrogen Receptor Subtypes and Hyperalgesic Priming
Cited 6 times
To investigate the role of estrogen receptor subtypes in hyperalgesic priming induced by ryanodine, antisense AS ODN against ERα and ERβ mRNA were administered to female rats. The sequence for the ERα, 5′-CAT-GGT-CAT-GGT-CAG-3, and the ERβ, 5′-GAA-TGT-CAT-AGC-TGA-3′, AS ODN (Invitrogen Life Technologies), were directed against unique regions of each rat ER subtype [GeneBank accession numbers NM_012689.1 (ERα) and NM_012754.1 (ERβ)], and have been previously shown to attenuate cellular levels of the respective ERs59 (link)60 (link). The MM ODN sequences, 5′-ATC-GTG-GAT-CGT-GAC-3′, for ERα, and 5′-AAG-GTT-ATC-GCA-AGT-3′, for ERβ, were scrambled AS ODN sequences that have the same base pairs and GC ratio, with the order randomized, and little or no homology to any mRNA sequences posted at GenBank.
Before use, ODNs were reconstituted in nuclease-free 0.9% NaCl, and then administered intrathecally at a dose of 2 μg/μl in a volume of 20 μl, for 3 consecutive days, starting 3 days before the injection of ryanodine, and then continued for 3 additional days, at which time the evaluation for the presence of priming was performed by intradermal administration of PGE2 on the dorsum of the hind paw. As described previously53 (link), rats were anesthetized with isoflurane (2.5% in O2), and the ODN injected using a microsyringe (10 μl) with a 30-gauge needle, inserted into the subarachnoid space, between the L4 and L5 vertebrae.
Ferrari L.F., Khomula E.V., Araldi D, & Levine J.D. (2016). Marked Sexual Dimorphism in the Role of the Ryanodine Receptor in a Model of Pain Chronification in the Rat. Scientific Reports, 6, 31221.
Full text: Click here
Publication 2016
5'-chloroacetamido-5'-deoxythymidine Cells Dinoprostone estrogen receptor alpha, human Females Homologous Sequences Hyperalgesia Isoflurane Needles Normal Saline Rattus norvegicus RNA, Messenger Ryanodine Subarachnoid Space Vertebra

Most recents protocols related to «Ryanodine»

1
Investigating Lipid-Mediated Cell Signaling
Sources for chemicals used in this study: Amitriptyline (Sigma Aldrich, Steinheim, Germany), BAPTA AM (Merck, Darmstadt, Germany), ceramide C16:0 and ceramide C24:1 (Cayman Chemical, Ann Arbor, MI, USA), DMSO (Sigma Aldrich), dodecane (Thermo Fisher Scientific, Waltham, MA, USA), ethanol (VWR, Radnor, PA, USA), fumonisin B1 (Cayman Chemical), hydrogen peroxide 3% (Sigma Aldrich), PDMP (Enzo Life Sciences, Farmingdale, NY, USA), 4-phenylbutyrate (Cayman Chemical, Ann Arbor, MI, USA), ryanodine (Santa Cruz, Dallas, TX, USA), sodium nitrite (Sigma Aldrich).
Zimmermann T., Staebler S., Taudte R.V., Ünüvar S., Grösch S., Arndt S., Karrer S., Fromm M.F, & Bosserhoff A.K. (2023). Cold Atmospheric Plasma Triggers Apoptosis via the Unfolded Protein Response in Melanoma Cells. Cancers, 15(4), 1064.
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Publication 2023
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 1-phenyl-2-decanoylamino-3-morpholino-1-propanol 4-phenylbutyrate Amitriptyline Caimans Ceramides Ethanol fumonisin B1 n-dodecane N-palmitoylsphingosine Peroxide, Hydrogen Peroxides Ryanodine Sodium Nitrite Sulfoxide, Dimethyl Tritium
2
Cell Treatment with Inhibitors and CAP
Unless otherwise stated, treatment with inhibitors began 24 h after seeding 200,000 cells in 35 mm cell culture dishes. The treatment procedure included 1 h of preincubation with each inhibitor, followed by CAP treatment and application of the inhibitor for one more hour. Preincubation was reduced to 30 min when using inhibitors of calcium metabolism (Ryanodine, BAPTA AM) to ensure comparability with previous studies [40 (link)]. PDMP was applied for a total of 48 h, starting with the seeding process and including a full change of cell culture medium and inhibitor after 24 h. Due to this increased incubation time, only 100,000 cells were seeded in 35 mm cell culture dishes for this experiment. In control experiments, cells were treated equally and received an appropriate amount of solvent.
Zimmermann T., Staebler S., Taudte R.V., Ünüvar S., Grösch S., Arndt S., Karrer S., Fromm M.F, & Bosserhoff A.K. (2023). Cold Atmospheric Plasma Triggers Apoptosis via the Unfolded Protein Response in Melanoma Cells. Cancers, 15(4), 1064.
Full text: Click here
Publication 2023
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 1-phenyl-2-decanoylamino-3-morpholino-1-propanol Calcium Cell Culture Techniques Cells Cultural Evolution Hyperostosis, Diffuse Idiopathic Skeletal inhibitors Metabolism Ryanodine Solvents
3
Measuring Calcium Dynamics in Skinned Muscle Fibers
Skeletal muscle fibers from WT mice were prepared as described (1 (link)). Briefly, a discrete section of isolated fibers were exposed to fluo-5N (1 mM) and RyR blocker ryanodine (50 µM). Fibers were then mechanically skinned and placed in a physiological solution containing 500 nM ERTY and incubated for 30 min at 37 °C. The solution was then replaced with a standard solution with 100 nM Ca2+ (no ERTY) and imaged on an Olympus FV1000 confocal microscope. ERTY fluorescence signal and temperature have been calibrated previously, with the signal changing at 3.9% per 1 °C (50 (link)).
Singh D.P., Pearce L., Choi R.H., Meizoso-Huesca A., Wette S.G., Scott J.W., Lamboley C.R., Murphy R.M, & Launikonis B.S. (2023). Evolutionary isolation of ryanodine receptor isoform 1 for muscle-based thermogenesis in mammals. Proceedings of the National Academy of Sciences of the United States of America, 120(4), e2117503120.
Publication 2023
A Fibers Fluorescence FLUOS Microscopy, Confocal Mus PER1 protein, human physiology Ryanodine Skeletal Myocytes
4
Multidrug Combination Therapy Evaluation
Doxorubicin (Dox, MB1087-1), Methotrexate (MTX, MB1156-1), Cisplatin (Cis, MB1055-1) and Paclitaxel (PTX, MB1178-1) were purchased from Meilunbio. Amiloride hydrochloride (PHR1839), NAC (A9165), DPI (43088), cyclosporin A (CsA, SML1018), EHT1864 (E1657), SKF-525A (567300), SB203580 (S8307), JSH-23 (J4455), PKH26-Red fluorescent cell linker kit, NH4Cl (A9434), PKH67-Green fluorescent cell linker kit and anti-Lamp1 antibody (L1418) were purchased from Sigma-Aldrich. Ryanodine (ab120083), CGP37157 (ab120012) were purchased from Abcam. Bafilomycin A1 (HY-100558) were purchased from MCE. ER-, Mito-, Lyso-Tracker Green/Red, LysoSensor Yellow/Blue DND-160 (L7545), Early Endosomes-GFP (C10586), LysoSensor Green DND-189 (L7535), CellLight Golgi-RFP (C10593) and Late Endosomes-GFP (C10588) were purchased from Thermo Fisher Scientific. Clodronate liposomes were purchased from Liposomal.
Wei K., Zhang H., Yang S., Cui Y., Zhang B., Liu J., Tang L., Tan Y., Liu S., Chen S., Yuan W., Luo X., Chen C., Li F., Liu J., Chen J., Xu P., Lv J., Tang K., Zhang Y., Ma J, & Huang B. (2023). Chemo-drugs in cell microparticles reset antitumor activity of macrophages by activating lysosomal P450 and nuclear hnRNPA2B1. Signal Transduction and Targeted Therapy, 8, 22.
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Publication 2023
2-(4-pyridyl)-5-((4-(2-dimethylaminoethylaminocarbamoyl)methoxy)phenyl)oxazole Antibodies, Anti-Idiotypic bafilomycin A1 Cells CGP37157 CISH protein, human Cisplatin Clodronate Cyclosporine EHT 1864 Endocytic Vesicles Endosomes Erythrocytes Golgi Apparatus Hydrochloride, Amiloride JSH 23 Liposomes LysoSensor Green DND-189 lysosomal-associated membrane protein 1, human LysoTracker Mitomycin Paclitaxel PKH 26 PKH67 Ryanodine SB 203580 SK&F-525-A
5
Saponin-based Cardiac Muscle Perfusion
Saponin (MP Biomedicals, USA) was dissolved in Tyrode’s solution with adequate ultrasonic agitation for 30 min. In addition, several experiments required the hearts to be perfused with Tyrode’s solution supplemented with various reagents including ryanodine (FUJIFILM Wako Pure Chemical Corporation, Japan), thapsigargin (FUJIFILM Wako Pure Chemical Corporation, Japan), MDL28170 (Sigma‒Aldrich, USA), 2,3-butanedione monoxime (BDM) (Nacalai Tesque, Japan) or carbonyl cyanide 4-trifluoromethoxy phenylhydrazone (FCCP) (Abcam, UK), for specific pharmacological interventions.
Morishita Y., Tamura S., Mochizuki K., Harada Y., Takamatsu T., Hosoi H, & Tanaka H. (2023). Generation of myocyte agonal Ca2+ waves and contraction bands in perfused rat hearts following irreversible membrane permeabilisation. Scientific Reports, 13, 803.
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Publication 2023
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone carbonyl cyanide phenylhydrazone diacetylmonoxime Heart MDL 28170 Ryanodine Saponin Thapsigargin Ultrasonics

Top products related to «Ryanodine»

1
Ryanodine by Bio-Techne
Sourced in United Kingdom, United States
Ryanodine is a chemical compound used in research and laboratory settings. It acts as a selective agonist for ryanodine receptors, which are responsible for the release of calcium from intracellular stores. Ryanodine binds to these receptors, modulating their activity and affecting cellular calcium signaling.
2
Ryanodine by Merck Group
Sourced in United States
Ryanodine is a laboratory reagent used in the study of ryanodine receptor channels, which are involved in the regulation of calcium signaling in various cell types. It functions as a selective ligand that binds to and modulates the activity of ryanodine receptors.
3
3h ryanodine by PerkinElmer
Sourced in United States
[3H]ryanodine is a radiolabeled compound used as a research tool to study ryanodine receptor channels, which play a crucial role in calcium signaling within cells. It can be utilized in various experimental techniques, such as binding assays and functional studies, to further understand the properties and regulation of ryanodine receptors.
4
Nifedipine by Merck Group
Sourced in United States, Germany, United Kingdom, China, Sao Tome and Principe, France, Italy, Australia, Canada, Israel, Belgium, Sweden, Brazil, Spain, India
Nifedipine is a pharmaceutical compound used in the production of lab equipment. It is a calcium channel blocker that can be used to regulate the flow of calcium into cells. The core function of Nifedipine is to control and maintain the balance of calcium levels within a controlled environment.
5
Caffeine by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Poland, Spain, Macao, Sao Tome and Principe, Belgium, Brazil, India, France, Australia, Argentina, Finland, Canada, Japan, Singapore, Israel
Caffeine is a naturally occurring stimulant compound that can be extracted and purified for use in various laboratory applications. It functions as a central nervous system stimulant, inhibiting the action of adenosine receptors in the brain.
6
Fluo 4 am by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Canada, China, France, Spain, Italy, Sweden, Japan, Switzerland, Belgium, Australia, Austria, Finland, New Zealand
Fluo-4 AM is a fluorescent calcium indicator used for the detection and measurement of intracellular calcium levels. It functions by binding to calcium ions, which results in an increase in fluorescence intensity. This product is commonly used in various cell-based assays and research applications involving calcium signaling.
7
Thapsigargin by Bio-Techne
Sourced in United Kingdom, United States
Thapsigargin is a selective inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump. It disrupts calcium homeostasis within the cell by blocking the uptake of calcium into the endoplasmic reticulum.
8
Thapsigargin by Merck Group
Sourced in United States, United Kingdom, Germany, Sao Tome and Principe, Italy, France, Japan, Canada, Spain, Macao, Belgium, Switzerland
Thapsigargin is a naturally occurring compound isolated from the plant Thapsia garganica. It functions as a selective inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump, which is responsible for the active uptake of calcium ions into the endoplasmic reticulum. Thapsigargin is a valuable tool for researchers studying calcium signaling and homeostasis in biological systems.
9
Fura 2 am by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Italy, France, Spain, Australia, Japan, China, Canada, Belgium, Austria, Hungary, Macao
Fura-2 AM is a fluorescent calcium indicator used for measuring intracellular calcium levels. It is a cell-permeable derivative of the parent compound Fura-2. Fura-2 AM can be loaded into cells, where intracellular esterases cleave off the acetoxymethyl (AM) ester group, trapping the Fura-2 indicator inside the cell.
10
2 apb by Merck Group
Sourced in United States, Germany, United Kingdom, Canada
2-APB is a chemical compound used as a laboratory reagent. It functions as an inhibitor of inositol 1,4,5-trisphosphate (IP3) receptors, which are responsible for the release of calcium from intracellular stores. The core function of 2-APB is to modulate cellular calcium signaling processes in various experimental settings.

More about "Ryanodine"

Ryanodine is a complex organic compound derived from the alkaloid ryanodine, which is extracted from the roots of certain plant species.
It acts as a specific agonist of the ryanodine receptor, a calcium release channel found in the sarcoplasmic reticulum of muscle cells.
Ryanodine binds to and modulates the activity of this receptor, playing a key role in the regulation of intracellular calcium homeostasis and excitation-contraction coupling in cardiac and skeletal muscle.
The ryanodine receptor (RyR) is a critical player in calcium signaling and muscle function. [3H]ryanodine, a radioactive analog of ryanodine, is a valuable tool used to study the distribution and characteristics of RyRs.
Calcium release through the RyR is also influenced by other compounds like Nifedipine, Caffeine, and 2-APB.
Intracellular calcium levels are further regulated by calcium-binding dyes like Fluo-4 AM and Fura-2 AM, as well as by the calcium ATPase inhibitor Thapsigargin.
Understanding the complex interplay between ryanodine, RyRs, and calcium signaling is crucial for the study and treatment of various muscular and cardiovascular disorders.
Ryanodine is a valuable tool for researchers, providing insights into the fundamental mechanisms of calcium homeostasis and muscle function.
Leveraging the power of AI-driven research optimization, tools like PubCompare.ai can help scientists identify the best protocols and products for improved reproducibility in their ryanodine-related studies.