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SCH 23390

SCH 23390 is a selective D1 dopamine receptor antagonist widely used in neuroscience research.
This powerful tool allows scientists to investigate the role of D1 receptors in a variety of neurological processes and disorders.
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Most cited protocols related to «SCH 23390»

1
Mapping Reward and Fear Behaviors in Rat Brain
Cited 9 times
Following behavioral testing, rats were deeply anesthetized with an overdose of sodium pentobarbital. Rats in which Fos plumes were measured were perfused and brains treated as described previously (Reynolds and Berridge, 2008 (link)). These included rats behaviorally tested in the environmental shift group (n=10; which therefore received a 7th final drug or vehicle microinjection and behavioral test 90 minutes prior to perfusion) and a separate dedicated Fos group (n = 36; which were histologically assessed after just a single drug or vehicle microinjection into locations staggered throughout medial shell, administered under conditions identical to the first day of testing for behavioral rats). The purpose of the dedicated Fos group was to assess maximal local impact radius, and avoid danger of under-estimating plume size due to progressive necrosis/gliosis over a series of microinjections that might shrink a final plume. If shrinkage occurred in the behaviorally tested group, that in turn could give rise to overly precise estimates of localization of function in brain maps. This potential distortion of impact estimates by plume shrinkage was prevented in the dedicated group that received only one microinjection.
All rats used for Fos analysis were anesthetized and transcardially perfused 90 minutes after their final or sole bilateral microinjection of vehicle (n=10), DNQX alone (n=13), DNQX plus SCH23390 (n=6), DNQX plus raclopride (n=10), DNQX plus raclopride and SCH23390 (n=3) or no solution (normal, n=3). Brain slices were processed for Fos-like immunoreactivity using NDS, goat anti-cfos (Santa Cruz Biotechnology, Santa Cruz, CA) and donkey anti-goat Alexa Fluor 488 (Invitrogen, Carlsbad, CA) (Faure et al., 2008 (link); Reynolds and Berridge, 2008 (link)). Sections were mounted, air-dried and coverslipped with ProLong Gold antifade reagent (Invitrogen). Zones where the expression of fluorescent Fos was elevated in neurons surrounding microinjection sites (“Fos plumes”) were assessed via microscope as described previously (Reynolds and Berridge, 2008 (link)).
Other brains were removed and fixed in 10% paraformaldehyde for 1–2 days and in 25% sucrose solution (0.1 M NaPB) for 3 days. For assessment of microinjection site locations in behaviorally tested rats, brains were sliced at 60 microns on a freezing microtome, mounted, air-dried and stained with cresyl violet for verification of microinjection sites. Bilateral microinjection sites for each rats were placed on coronal slices from a rat brain atlas (Paxinos and Watson, 2007 ), which were used to extrapolate the position of each site on one sagittal slice. Mapping in the sagittal view allows for the presentation on the same map of the entire rostrocaudal and dorsoventral extents of NAc medial shell. Functional effects on appetitive and fearful behaviors were mapped using color-coding to express the intensity of changes in motivated behaviors for individual behaviorally-tested rats. Symbols were sized to match the maximal diameter of Fos plumes measured as described below. Sites were classified as rostral shell if their NAc placements were located +1.4 to +2.6 mm ahead of bregma, and as caudal shell if their placements were located +0.4 to +1.4 mm ahead of bregma.
Richard J.M, & Berridge K.C. (2011). Nucleus Accumbens Dopamine/Glutamate Interaction Switches Modes to Generate Desire versus Dread: D1 Alone for Appetitive Eating But D1 and D2 Together for Fear. The Journal of Neuroscience, 31(36), 12866-12879.
Publication 2011
6,7-dinitroquinoxaline-2,3-dione alexa fluor 488 Brain Brain Mapping cresyl violet Drug Overdose Equus asinus Fear Gliosis Goat Gold Microinjections Microscopy Microtomy Necrosis Neurons paraform Pentobarbital Sodium Perfusion Pharmaceutical Preparations Raclopride Radius Rattus norvegicus SCH 23390 Sucrose
2
Cocaine Self-Administration and Motivation
Cited 8 times

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Publication 2013
Animals Brain Cannulation Catheterization Cocaine Females Men Motivation neuro-oncological ventral antigen 2, human Operative Surgical Procedures Rattus norvegicus SCH 23390 Self Administration Sucrose
3
Rotarod Task for Evaluating Dopamine Receptor Function
Cited 8 times
A computer-interfaced rotarod accelerating from 4–40 rotations per min over 300 s was used (ENV-575M, Med Associates). Animals were trained with ten trials per day for either 1 d or 8 d (trained every other day). This training protocol was chosen on the basis of studies determining the time course of sensitivity of this task to interference, protein synthesis blockers (data not shown) and dopamine receptor antagonists (Fig. 5d). Each trial ended when the mouse fell off the rotarod or after 300 s had elapsed and there was a resting period of approximately 300 s between trials. Yoked animals were handled and placed in the rotarod in the same manner as the trained animals, but without the rotation of the rod. During the in vivo recordings, the beginning and end of the running period were signaled to the MAP recording system (Plexon) as events. The D1 receptor antagonist SCH-23390 (0.4 mg per kg of body weight, Sigma-Aldrich) and D2 receptor antagonist raclopride (2.0 mg per kg, Sigma-Aldrich) were dissolved in phosphate-buffered saline with 1% DMSO by volume (control injection) and injected intra-peritoneally at 10 ml kg–1 (these doses completely block the effects of 3,4-dihydroxy-l-phenylalanine after dopamine depletion49 (link), indicating complete block of D1 and D2 receptors in vivo).
Yin H.H., Mulcare S.P., Hilário M.R., Clouse E., Holloway T., Davis M.I., Hansson A.C., Lovinger D.M, & Costa R.M. (2009). Dynamic reorganization of striatal circuits during the acquisition and consolidation of a skill. Nature neuroscience, 12(3), 333-341.
Publication 2009
A 300 Animals Body Weight Cardiac Arrest Dopamine Dopamine Antagonists Dopamine D1 Receptor Dopamine D2 Receptor Hypersensitivity Levodopa Mice, House Phosphates Protein Biosynthesis Raclopride Saline Solution SCH 23390 Sulfoxide, Dimethyl
4
Microinjection of Neuromodulators in Honeybees
Cited 8 times
Drugs were dissolved in honeybee Ringer [NaCl 130 mM, KCl 6 mM, MgCl2 4 mM, CaCl2 5 mM, HEPES 10 mM, glucose 25 mM, sucrose 160 mM]. Drugs or Ringer alone (control) were injected into the brain through the median ocellar tract. In addition to normal preparation, the head of each harnessed bee was fastened to the holder with a small drop of wax to avoid movements. A Harvard GC 100-10 microelectrode filled with the drug to be injected was connected to an IM 300 Narishige microinjector and used to deliver 10×20 nl into the brain. Volumes injected were calibrated before and after injection by means of a Malassez cell. Injections were performed 30 min before conditioning, as earlier experiments (reviewed in ref. 42) showed that pharmacological injections of catecholamines and their inhibitors are effective approximately 30 min after drug application. Octopaminergic (mianserine, epinastine) and dopaminergic receptor antagonists (fluphenazine, flupentixol, SCH23390, spiperone) (Sigma Aldrich, Deisenhofen, Germany) were used at µm and mM doses (Fig. 6). Low-dose experiments (except for flupenthixol 0.2 µm) and their respective Ringer control were performed in parallel (yellow rows in Fig. 6). High-dose experiments (and flupentixol 0.2 µm) were also performed in parallel with their respective Ringer control (pink rows in Fig. 6). The group injected with epinastine received one dose and had a separate Ringer control (blue row in Fig. 6).
Vergoz V., Roussel E., Sandoz J.C, & Giurfa M. (2007). Aversive Learning in Honeybees Revealed by the Olfactory Conditioning of the Sting Extension Reflex. PLoS ONE, 2(3), e288.
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Publication 2007
Brain Catecholamines Cells Dopamine Antagonists epinastine Flupenthixol Fluphenazine Glucose Head HEPES inhibitors Magnesium Chloride Mianserin Microelectrodes Movement Pharmaceutical Preparations SCH 23390 Sodium Chloride Spiperone Sucrose
5
Investigating Drug Effects on Habit Formation
Cited 7 times
During degradation sessions, conditions were identical to training except that the grain pellet reinforcer was delivered on a non-contingent schedule determined by each individual animal's reinforcement rate on the day prior. Reinforcer delivery was spaced equivalently across the 30-min session. Responses on the active and inactive nosepokes were recorded, but did not result in reinforcer delivery. Infusions of drugs occurred 5 min prior to the start of the degradation session. Mice were assigned to infusion groups by matching baseline response rates, and received a 0.2 ul infusion of either saline (n = 17), DHX (n = 11), SCH23390 (n = 6), quinpirole (n = 12), or sulpiride (n = 9). More animals were in the saline groups as a cohort of saline animals was included with each behavioral test session to confirm baseline effects were replicated. Data were compared to a non-degraded session in which the animals received the same drug; the order of these sessions was counterbalanced and animals received one normal RI 60 training session between both test sessions where no drug was administered.
Barker J.M., Torregrossa M.M, & Taylor J.R. (2013). Bidirectional modulation of infralimbic dopamine D1 and D2 receptor activity regulates flexible reward seeking. Frontiers in Neuroscience, 7, 126.
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Publication 2013
Animals Behavior Test Cereals Mice, House Obstetric Delivery Pharmaceutical Preparations Quinpirole Reinforcement, Psychological Saline Solution SCH 23390 Sulpiride

Most recents protocols related to «SCH 23390»

1
Measuring D1DR Binding Density in Brain
Brain regional D1DR-binding densities were examined as described previously [23 (link),26 (link)]. Slide-mounted sections were incubated in Tris-buffer (50 mM Tris–HCl containing 120 mM NaCl, 5 mM KCl, 2 mM CaCl2, and 1 mM MgCl2; pH 7.7) containing 0.2 nM [3H]SCH 23390 (specific activity 66.0 Ci/mmol, Amersham (Amersham, UK)) for 90 min at room temperature. Sections for non-specific binding were incubated in Tris-buffer containing 0.2 nM [3H]SCH 23390 and 10−7 M cis-flupenthixol. After incubation, the slides were drained, washed twice for 5 min in buffer at +4 °C and briefly dipped twice into distilled water (+4 °C). Sections were dried at room temperature overnight and exposed to a tritium-sensitive film (3H Hyperfilm®, Amersham) at −20 °C for 6 weeks, together with Amersham 3H Microscale Autoradiography Standards®.
Tsyba E.T., Midzyanovskaya I.S., Birioukova L.M., Tuomisto L.M., van Luijtelaar G, & Abbasova K.R. (2023). Striatal Patchwork of D1-like and D2-like Receptors Binding Densities in Rats with Genetic Audiogenic and Absence Epilepsies. Diagnostics, 13(4), 587.
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Publication 2023
Autoradiography Brain Buffers Flupenthixol Magnesium Chloride SCH 23390 Sodium Chloride Tritium Tromethamine
2
Spatial Correlation of Brain-Fingerprints and Neuroreceptor Maps
Using a similar approach as in the above subsection, we estimated the Pearson’s spatial correlation between the regional ΔICC values of the brain-fingerprints and each of the normative atlas maps (from neuromaps36 (link)) of 19 receptors and transporters from 9 neurotransmitter systems. These latter consisted of: dopamine (D1: 13 adults, [11C]SCH23390 PET; D2: 92, [11C]FLB-457, DAT: 174, [123I]-FP-CIT), serotonin (5-HT1a: 36, [11C]WAY-100635; 5-HT1b: 88, [11C]P943; 5-HT2a: 29, [11C]Cimbi-36; 5-HT4: 59, [11C]SB207145; 5-HT6: 30, [11C]GSK215083; 5-HTT: 100, [11C]DASB), acetylcholine (α4β2: 30, [18F]flubatine; M1: 24, [11C]LSN3172176; VAChT: 30, [18F]FEOBV), GABA (GABAa: 16, [11C]flumazenil), glutamate (NMDA: 29, [18F]GE-179; mGluR5: 123, [11C]ABP688), norepinephrine (NET: 77, [11C]MRB), histamine (H3: 8, [11 C]GSK189254), cannabinoid (CB1: 77, [11 C]OMAR), and opioid (MOR: 204, [11 C]carfentanil).
Each map was parcellated using the 68 regions of the Desikan-Killiany atlas38 (link). Statistical significance was determined after correction for multiple comparisons using a False Discovery Rate (FDR) as implemented in the R function p.adjust98 . We derived Bayes factors to quantify the evidence in favour of the alternative hypothesis (i.e., a spatial correlation does exist) using the correlationBF function in R. For each significant spatial correspondence observed, we also estimated p-values based on spatially constrained permutation tests96 (link),97 (link). We performed 1000 permutations of the labels of patients vs. controls, computed a corresponding null ICC matrix for the patients and controls groups, and estimated the correlation of the resulting random differences in ICC for these null models with each of the 19 neurotransmitter atlas maps.
da Silva Castanheira J., Wiesman A.I., Hansen J.Y., Misic B, & Baillet S. (2023). The neurophysiological brain-fingerprint of Parkinson’s disease. medRxiv.
Publication Preprint 2023
3-(6-methylpyridin-2-ylethynyl)-cyclohex-2-enone-O-11C-methyl-oxime 123I-FP-CIT Acetylcholine Adult Brain Cannabinoids carfentanil Cimbi-36 Dopamine exo-2-(2'-fluoro-5'-pyridinyl)-7-azabicyclo(2.2.1)heptane FLB 457 Flumazenil gamma Aminobutyric Acid Glutamate GRM5 protein, human GSK 189254 GSK215083 Histamine HTR1A protein, human HTR1B protein, human LSN3172176 Microtubule-Associated Proteins N-Methylaspartate Neurotransmitters Neurotransmitter Transport Proteins Norepinephrine Opioids Patients SB 207145 SCH 23390 Serotonin WAY 100635
3
Investigating Matcha Tea Effects
SCH23390 hydrochloride (Cayman Chemical, Ann Arbor, MI, USA) was dissolved in saline at 0.05 mg/mL and intraperitoneally administered at a dose of 50 μg/kg 30 min before the oral administration of Matcha tea powder. As control experiments, the same volume of saline was administered intraperitoneally.
Kurauchi Y., Ohta Y., Matsuda K., Sanematsu W., Devkota H.P., Seki T, & Katsuki H. (2023). Matcha Tea Powder’s Antidepressant-like Effect through the Activation of the Dopaminergic System in Mice Is Dependent on Social Isolation Stress. Nutrients, 15(3), 581.
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Publication 2023
Administration, Oral Caimans Powder Saline Solution SCH 23390
4
Acute and Chronic Effects of Alpha-Synuclein on Neuronal Activity
Micro-electrode arrays were purchased from Multichannel Systems (MCS, Reutlingen, Germany). MEAs consist of 60 TiN (titanium nitride) planar round electrodes (30 μm diameter; 200 μm center-to-center inter-electrode distance). MEA amplifier was kept inside an incubator with a controlled temperature (37°C) and humified atmosphere (i.e., gas flow of 5% CO2 and 95% O2). All measurements were performed by keeping the neurons in their culture medium. Acquired signals, after 1,200× amplification, were sampled at 10 kHz and acquired through the data acquisition hardware and MC-Rack software (MCS). For each trial, data acquisition was performed over 2 min recordings.
All experiments using drugs have been performed by adding the drugs to the culture medium under static conditions, without superfusion. For acute application, measurement started 5 min after drugs administration, in order to restore temperature and CO2 conditions inside the incubator.
L-DOPA was purchased from Sigma-Aldrich (SIGMA, St. Louis, MO, USA) and used at 20 μM final concentration. D1 (SCH-23390) and D2 (sulpiride) receptors antagonist were purchased from Sigma-Aldrich (SIGMA, St. Louis, MO, USA) and used at 10 μM final concentration.
The experiments with α-synuclein (S7820, Sigma-Aldrich, Merck Darmstadt Germany) were carried out using different concentrations (0.3, 0.5, 1, 3, and 70 μM). Recordings were performed in three conditions: acute applications (see below for details), 24 and 48 h after α-synuclein addition.
Tomagra G., Franchino C., Cesano F., Chiarion G., de lure A., Carbone E., Calabresi P., Mesin L., Picconi B., Marcantoni A, & Carabelli V. (2023). Alpha-synuclein oligomers alter the spontaneous firing discharge of cultured midbrain neurons. Frontiers in Cellular Neuroscience, 17, 1078550.
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Publication 2023
Atmosphere Culture Media Levodopa Neurons Pharmaceutical Preparations SCH 23390 SNCA protein, human Sulpiride titanium nitride Training Programs
5
Osteogenic Induction with Dopamine Receptor Modulation
The MC3T3-E1 cells (Rockville, Maryland, USA) and BMSC cells (MUBMX-01001, Cyagen, Guangzhou, China) were grown in medium containing 10% FBS (16140071, Rockville, USA). Osteogenic induction medium (DMEM, SH30022.01, Cytiva, Pittsburgh, USA) supplemented with 10% FBS, 100 nm dexamethasone (D1756, Sigma-Aldrich, MO, USA), 10 mm β-glycerophosphate (G9422, Sigma-Aldrich, MO, USA), and 50 mm vitamin C (PHR1008, Sigma-Aldrich, MO, USA). To mimic the osteolytic microenvironment, 5 μg/cm2 of titanium particles were added to the culture medium. Then, the cells were pretreated with either a D1R agonist (10 μM, SKF38393) or a D1R agonist (10 μM) plus an inhibitor (0.2 nm, SCH23390).
Feng C., Li Y., Gu M., Li W., Yang Y., Chen S., Ma Y., Geng D., Xiao L, & Wang Z. (2023). The Dopamine D1 Receptor Attenuates Titanium Particle-Induced Inhibition of Osteogenesis by Activating the Wnt Signaling Pathway. Mediators of Inflammation, 2023, 6331650.
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Publication 2023
Ascorbic Acid beta-glycerol phosphate Cells Culture Media Dexamethasone Osteogenesis Osteolysis SCH 23390 SK&F-38393 Titanium

Top products related to «SCH 23390»

1
Sch23390 by Merck Group
Sourced in United States, United Kingdom
SCH23390 is a laboratory reagent used for scientific research. It is a specific antagonist of the D1 dopamine receptor, and is commonly used as a tool compound in neuroscience and biochemistry studies. The core function of SCH23390 is to selectively bind to and block the activity of the D1 dopamine receptor in in vitro and in vivo experimental settings.
2
Sch23390 by Bio-Techne
Sourced in United Kingdom, United States
SCH23390 is a selective dopamine D1 receptor antagonist. It binds to the D1 dopamine receptor with high affinity and selectivity, making it a useful tool for studying the function and distribution of this receptor subtype.
3
R sch 23390 hydrochloride by Merck Group
Sourced in United States, Germany
R(+)-SCH-23390 hydrochloride is a chemical compound that functions as a selective dopamine D1 receptor antagonist. It is commonly used as a research tool in pharmacological studies to investigate the role of dopamine D1 receptors in various biological processes.
4
Sulpiride by Bio-Techne
Sourced in United Kingdom, United States
Sulpiride is a laboratory reagent used for analytical and research purposes. It is a selective dopamine D2 receptor antagonist. Sulpiride is commonly used in various research applications, including neuroscience and pharmacology studies.
5
Sch 23390 hydrochloride by Bio-Techne
Sourced in United Kingdom
SCH 23390 hydrochloride is a dopamine D1 receptor antagonist used in scientific research. It is a white crystalline powder that is soluble in water and DMSO. The product is supplied as a solid.
6
Haloperidol by Merck Group
Sourced in United States, United Kingdom, Sao Tome and Principe, Germany, France, Japan, Spain
Haloperidol is a laboratory reagent used in various research and analytical applications. It is a butyrophenone-class antipsychotic drug that acts as a dopamine D2 receptor antagonist. Haloperidol is commonly used as a reference standard and in the development and validation of analytical methods.
7
3h sch23390 by PerkinElmer
Sourced in United States, Germany, France
[3H]SCH23390 is a high-affinity and selective dopamine D1 receptor radioligand. It is commonly used in receptor binding assays to study the distribution and pharmacology of dopamine D1 receptors in the central nervous system.
8
Sulpiride by Merck Group
Sourced in United States, United Kingdom, Brazil
Sulpiride is a laboratory product provided by Merck Group. It is a chemical compound primarily used in research and analytical applications. The core function of Sulpiride is to serve as a reference standard or analytical tool, without further interpretation of its intended use.
9
Raclopride by Merck Group
Sourced in United States
Raclopride is a selective dopamine D2 receptor antagonist used in research applications. It is commonly utilized in various scientific studies, including neuroimaging and pharmacological investigations.
10
Quinpirole by Bio-Techne
Sourced in United Kingdom, United States
Quinpirole is a synthetic chemical compound that is commonly used as a laboratory tool in scientific research. It functions as a selective agonist for the D2 and D3 dopamine receptors, which are important in the study of the dopaminergic system and its role in various biological processes and neurological disorders.

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