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Sinapinic acid

Sinapinic acid, also known as 3,5-dimethoxy-4-hydroxycinnamic acid, is a naturally occurring phenolic compound found in various plants.
It has been studied for its potential pharmaceutical and research applications, including its use as a matrix in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis.
Sinapinic acid exhibits antioxidant, anti-inflammatory, and antimicrobial properties, making it a valuable compound in the field of medicinal chemistry.
Researchers can utilize PubCompare.ai's AI-driven platform to easily locate, compare, and optimize protocols for working with sinapinic acid, saving time and improving research outcomes.

Most cited protocols related to «Sinapinic acid»

Comprehensive Phytochemical and Antioxidant Analysis

In this study, most of the chemicals, reagents, and standards were analytical grade and purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Gallic acid, L-ascorbic acid, vanillin, hexahydrate aluminium chloride, Folin-Ciocalteu’s phenol reagent, sodium phosphate, iron(III) chloride hexahydrate (Fe[III]Cl₃.6H₂O), hydrated sodium acetate, hydrochloric acid, sodium carbonate anhydrous, ammonium molybdate, quercetin, catechin, 2,2′-diphenyl-1-picrylhy-drazyl (DPPH), 2,4,6tripyridyl-s-triazine (TPTZ), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from the Sigma-Aldrich (Castle Hill, NSW, Australia) for the estimation of polyphenols and antioxidant potential. Sulfuric acid (H₂SO₄) with 98% purity was purchased from RCI Labscan (Rongmuang, Thailand). HPLC standards including gallic acid, p-hydroxybenzoic acid, caftaric acid, caffeic acid, protocatechuic acid, sinapinic acid, chlorogenic acid, syringic acid, ferulic acid, coumaric acid, catechin, quercetin, quercetin-3-galactoside, diosmin, quercetin-3-glucuronide, epicatechin gallate, quercetin-3-glucoside, kaempferol and kaempferol-3-glucoside were produced by Sigma-Aldrich (Castle Hill, NSW, Australia) for quantification proposes. HPLC and LC-MS grade reagents including methanol, ethanol, acetonitrile, formic acid, and glacial acetic acid were purchased from Thermo Fisher Scientific Inc. (Scoresby, VIC, Australia). To perform various in vitro bioactivities and antioxidant assays, 96 well-plates were bought from the Thermo Fisher Scientific (VIC, Australia). Additionally, HPLC vials (1 mL) were procured from the Agilent technologies (VIC, Australia).

Suleria H.A., Barrow C.J, & Dunshea F.R. (2020). Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels. Foods, 9(9), 1206.

Publication 2020

2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 4-hydroxybenzoic acid Acetic Acid acetonitrile Aluminum Chloride ammonium molybdate Antioxidants Ascorbic Acid Biological Assay caffeic acid caftaric acid Catechin Chlorides Chlorogenic Acid Coumaric Acids Diosmin diphenyl epicatechin-3-gallate Ethanol ferulic acid folin formic acid Gallic Acid Glucosides High-Performance Liquid Chromatographies Hydrochloric acid hyperoside Iron isoquercetin kaempferol Methanol Phenol Polyphenols protocatechuic acid Quercetin quercetin 3-O-glucuronide sinapinic acid Sodium Acetate sodium carbonate sodium phosphate Sulfonic Acids Sulfuric Acids syringic acid Triazines vanillin

MALDI-TOF Mass Spectrometry Imaging

Cryosections of the tissues were cut in a cryo-microtome (Leica CM1900-UV) at a thickness of 10 μm and transferred onto conductive indium-tin-oxide-coated glass slides (Bruker Daltonik, Bremen, Germany). The sections were vacuum-dried in a desiccator for approximately 15 min then washed two times in 70% ethanol and once in 96% ethanol for 1 min each. The sections were then dried and stored under vacuum until the matrix was applied.
The sections were coated with matrix using an ImagePrep (Bruker Daltonik) according to the manufacturer's standard protocols. The brain and testis samples were coated with α-cyano-4-hydroxy-cinnamic acid (Bruker Daltonik), while the pancreas sample was coated with sinapinic acid (Bruker Daltonik).
All mass spectra were acquired in linear mode on autoflex or ultraflex instruments equipped with smartbeam (pancreas) or smartbeam II lasers (all other samples; Bruker Daltonik). For each pixel, 200 laser shots were accumulated at constant laser energy.

Deininger S.O., Cornett D.S., Paape R., Becker M., Pineau C., Rauser S., Walch A, & Wolski E. (2011). Normalization in MALDI-TOF imaging datasets of proteins: practical considerations. Analytical and Bioanalytical Chemistry, 401(1), 167-181.

Publication 2011

Brain cinnamic acid Cryoultramicrotomy Electric Conductivity Ethanol indium tin oxide Mass Spectrometry Microtomy Pancreas sinapinic acid Testis Tissues Vacuum

MALDI-TOF Mass Spectrometry for VeriStrat Classification

VeriStrat analysis was conducted on 441 available plasma samples by Biodesix (Broomfield, CO). Samples were thawed on wet ice and aliquots diluted 1:10 in HPLC-grade water (Burdick & Jackson, Muskegon, MI) then combined with an equal volume of sinapinic acid (Sigma, St. Louis, MO) solution (25 mg/ml sinapinic acid prepared in 50% acetonitrile/0.1% [Burdick & Jackson, Muskegon, MI] trifluoroacetic acid [Sigma,St. Louis, MO]). Each sample-matrix mixture was spotted in triplicate at randomly assigned positions on polished stainless steel MALDI plates (BrukerDaltonics, Bremen, Germany). Positive ion mass spectra for all samples and replicates were acquired in linear mode using the BrukerAutoflex III mass spectrometer. Averaged spectra, consisting of 2000 independent spectrum acquisitions, from each sample replicate were used for processing and classification. Spectral processing included background (BG) and noise estimation, BG subtraction, normalization to partial ion current and alignment. The classification algorithm, a KNN classifier based on eight distinct m/z features,^{5 (link)} was applied to the averaged, processed spectra. A VeriStrat label of Good or Poor was produced for each sample when all replicates from a sample gave the same classification. When replicates from a sample gave discordant classifications an indeterminate label was assigned. Results were sent to NCIC CTG where they were merged with the clinical trial database.

Carbone D.P., Ding K., Roder H., Grigorieva J., Roder J., Tsao M.S., Seymour L, & Shepherd F.A. (2012). Prognostic and Predictive Role of the VeriStrat® Plasma Test in Patients with Advanced Non-Small Cell Lung Cancer Treated with Erlotinib or Placebo in the NCIC Clinical Trials Group BR.21 Trial. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 7(11), 1653-1660.

Publication 2012

acetonitrile DNA Replication High-Performance Liquid Chromatographies Ion Transport Mass Spectrometry Plasma sinapinic acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stainless Steel Trifluoroacetic Acid

Serum Proteomic Profiling for Tuberculosis Diagnosis

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Publication 2006

Immunoaffinity Purification and Mass Spectrometry

Substrates from in vitro proteolysis assays or transformed/transfected cells (lysed in RIPA buffer) were subjected to anti-Flag immunoaffinity purification with the M2 resin (Sigma, St Louis, MO), and analyzed by MALDI-TOF mass spectrometry using sinapinic acid as the matrix as described previously (Baker et al., 2007 (link)).

Moin S.M, & Urban S. (2012). Membrane immersion allows rhomboid proteases to achieve specificity by reading transmembrane segment dynamics. eLife, 1, e00173.

Publication 2012

Biological Assay Buffers Cells Mass Spectrometry Proteolysis Radioimmunoprecipitation Assay Resins, Plant sinapinic acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Most recents protocols related to «Sinapinic acid»

MALDI-ToF MS Protein Sample Preparation

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Publication 2024

MALDI-TOF Mass Spectrometry Sample Preparation

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Publication 2024

DSPE-PEG2000-NOTA Conjugation Verification

Conjugation of DSPE-PEG2000-NOTA was confirmed by matrix-assisted laser desorption ionization/time of flight (MALDI/TOF) mass spectroscopy. The purified product (1 mg) was added to an ultraflextreme (Bruker Daltonics, Billerica, MA, USA) mass spectrometer using sinapinic acid as a matrix (Bruker Daltonics) in Korea Basic Science Institute (KBSI, Daejeon, Republic of Korea).

Jeong H.Y., Kang S.J., Kim M.W., Jeong I.H., Choi M.J., Jung C., Song I.H., Lee T.S, & Park Y.S. (2024). Development of PET Radioisotope Copper-64-Labeled Theranostic Immunoliposomes for EGFR Overexpressing Cancer-Targeted Therapy and Imaging. International Journal of Molecular Sciences, 25(3), 1813.

Publication 2024

MALDI-TOF-MS Protein Analysis Protocol

MALDI-TOF-MS was conducted on a
Bruker Microflex LRF instrument
with a microScout ion source. A saturated solution of sinapinic acid
(10 mg/mL in 70% acetonitrile in water +0.1% TFA) was used as the
matrix. Samples were prepared by a serial dilution of the matrix.
The solutions were dispensed onto a sample plate and dried at room
temperature. Apomyoglobin (MW = 16,952.27 Da) and aldolase (MW = 39, 211
Da) were used to calibrate the instrument.

Zhang Z., Lynch C.J., Huo Y., Chakraborty S., Cremer P.S, & Mozhdehi D. (2024). Modulating Phase Behavior in Fatty Acid-Modified Elastin-like Polypeptides (FAMEs): Insights into the Impact of Lipid Length on Thermodynamics and Kinetics of Phase Separation. Journal of the American Chemical Society, 146(8), 5383-5392.

Publication 2024

Protein Purification and Characterization Protocol

Carboxylic
acids (C2:0-C16:0), triethylamine,
apomyoglobin, aldolase, sinapinic acid, trifluoroacetic acid (TFA),
and syringe filters were purchased from Sigma-Aldrich (St. Louis,
MO). Acetonitrile and SnakeSkin dialysis tubing with a 3.5 kDa nominal
molecular weight cutoff, tryptone, yeast extract, sodium chloride,
kanamycin, and phosphate-buffered saline (PBS) were purchased from
Thermo Fisher Scientific (Rockford, IL). Poly(N-isopropylacrylamide)
(PNIPAM) and poly(N,N-dimethylacrylamide)
(PDMA) were purchased from Polymer Source (Quebec, CA). Chemically
competent BL21(DE3) cells were purchased from New England Biolabs
(Ipswich, MA). Deionized water was obtained using a Milli-Q system
(Millipore SAS, France). All chemicals were used as received without
further purification.

Publication 2024

Top products related to «Sinapinic acid»

Sinapinic acid by Merck Group

Sourced in United States, Germany, Italy, France

Sinapinic acid is a type of phenolic compound commonly used as a matrix in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It facilitates the ionization of various analytes, including proteins, peptides, and small molecules, for their detection and analysis using MALDI-MS techniques.

Trifluoroacetic acid by Merck Group

Sourced in United States, Germany, United Kingdom, France, Spain, Italy, Australia, China, India, Sao Tome and Principe, Poland, Canada, Switzerland, Macao, Belgium, Netherlands, Czechia, Japan, Austria, Brazil, Denmark, Ireland

Trifluoroacetic acid is a colorless, corrosive liquid commonly used as a reagent in organic synthesis and analytical chemistry. It has the chemical formula CF3COOH.

Acetonitrile by Merck Group

Sourced in Germany, United States, Italy, India, China, United Kingdom, France, Poland, Spain, Switzerland, Australia, Canada, Brazil, Sao Tome and Principe, Ireland, Belgium, Macao, Japan, Singapore, Mexico, Austria, Czechia, Bulgaria, Hungary, Egypt, Denmark, Chile, Malaysia, Israel, Croatia, Portugal, New Zealand, Romania, Norway, Sweden, Indonesia

Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.

Flexanalysis 3 by Bruker

Sourced in Germany, United States, United Kingdom

FlexAnalysis 3.4 is a software package designed for the analysis and processing of mass spectrometry data. It provides users with a comprehensive suite of tools for data visualization, peak detection, and quantification.

Flexanalysis software by Bruker

Sourced in Germany, United States

FlexAnalysis is a software platform developed by Bruker that provides data analysis tools for various analytical techniques. It offers a user-friendly interface and a comprehensive set of features to process and interpret data from Bruker's analytical instruments.

Sinapinic acid by Bruker

Sourced in Germany, United States

Sinapinic acid is a chemical compound commonly used as a matrix in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It assists in the ionization of analytes, enabling their detection and analysis by the mass spectrometer.

P coumaric acid by Merck Group

Sourced in United States, Germany, Italy, Spain, France, China, Poland, United Kingdom, Sao Tome and Principe, Switzerland, Canada, Ireland, India, Australia, Japan, Macao, Portugal

P-coumaric acid is a naturally occurring phenolic compound that can be utilized as a reference standard or an analytical reagent in various laboratory settings. It is a white to off-white crystalline solid that is soluble in organic solvents. P-coumaric acid is commonly used as a standard in analytical techniques, such as high-performance liquid chromatography (HPLC) and spectrophotometric measurements, to quantify and characterize similar compounds in sample matrices.

Flexcontrol 3 by Bruker

Sourced in Germany, United States

FlexControl 3.4 is a software package developed by Bruker for controlling various analytical instruments. It provides a user-friendly interface for configuring and operating the instruments, allowing researchers to automate and streamline their experimental processes.

Ferulic acid by Merck Group

Sourced in United States, Germany, Italy, United Kingdom, Spain, China, France, Poland, Australia, Ireland, Malaysia, Canada, India, Switzerland, Sao Tome and Principe, Japan, Brazil, Denmark

Ferulic acid is a phenolic compound that can be found in various plant sources, including rice, wheat, oats, and vegetables. It is commonly used as a lab equipment product for research and analysis purposes. Ferulic acid has antioxidant properties and can be used in a variety of applications, such as the study of plant-based compounds and their potential health benefits.

α cyano 4 hydroxycinnamic acid by Merck Group

Sourced in United States, Germany, France, United Kingdom, Japan, Sao Tome and Principe, Poland, Macao, Saudi Arabia

α-cyano-4-hydroxycinnamic acid is a chemical compound used in various laboratory applications. It serves as a matrix for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry analysis. The compound facilitates the ionization of analyte molecules during the MALDI process.

What are the key properties and applications of Sinapinic acid?

Sinapinic acid, also known as 3,5-dimethoxy-4-hydroxycinnamic acid, is a naturally occurring phenolic compound found in various plants. It exhibits potent antioxidant, anti-inflammatory, and antimicrobial properties, making it a valuable compound in medicinal chemistry and pharmaceutical research. Sinapinic acid is commonly used as a matrix in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, a technique that helps researchers identify and characterize biomolecules with high sensitivity and accuracy.

What are some common challenges researchers face when working with Sinapinic acid?

One of the main challenges with Sinapinic acid is optimizing the protocols and methods for its effective use. Researchers may struggle to identify the most efficient and reproducible procedures for tasks such as sample preparation, MALDI-MS analysis, and extraction. Additionally, there can be variability in the quality and purity of Sinapinic acid from different sources, which can impact experimental results.

How can PubCompare.ai help researchers working with Sinapinic acid?

PubCompare.ai's AI-driven platform can be extremely helpful for researchers working with Sinapinic acid. The platfrom allows you to efficiently screen protocol literature, leverage AI to pinpoint critical insights, and identify the most effective protocols related to Sinapinic acid for your specific research goals. The platform's advanced analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy, thus saving time and improving your research outcomes.

Are there different variations or types of Sinapinic acid available?

Yes, there are several different varations and types of Sinapinic acid available. While the basic chemical structure remains the same, some common variations include different purity levels, forms (e.g. crystalline, amorphous), and sourcing from different plant species. Researchers should carefuly evaluate the specific properties and quality of the Sinapinic acid they use to ensure consistent and reliable results in their experiments.

What are some specific applications of Sinapinic acid in research and industry?

In additoin to its use as a MALDI-MS matrix, Sinapinic acid has a range of other applications in research and industry. It has been studied for its potential pharmaceutical applications, such as its use as an antioxdant, anti-inflammatory, and antimicrobial agent. Sinapinic acid is also used in the production of certain cosmetic and personal care products due to its natural origin and beneficial properties. Reserachers can utilize PubCompares.ai's AI-driven platform to optimize protocols and methods for working with Sinapinic acid across these diverse applications.

More about "Sinapinic acid"

Sinapinic acid, also known as 3,5-dimethoxy-4-hydroxycinnamic acid, is a naturally occurring phenolic compound that can be found in various plants.
It has garnered significant attention in the scientific community due to its potential pharmaceutical and research applications.
One of the notable uses of sinapinic acid is as a matrix in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, a powerful analytical technique.
Sinapinic acid exhibits a range of beneficial properties, including antioxidant, anti-inflammatory, and antimicrobial activities, making it a valuable compound in the field of medicinal chemistry.
Researchers can leverage the power of PubCompare.ai's AI-driven platform to easily locate, compare, and optimize protocols for working with sinapinic acid, saving time and improving research outcomes.
In addition to sinapinic acid, other related compounds such as trifluoroacetic acid, acetonitrile, and p-coumaric acid are commonly used in MALDI-MS analysis and sample preparation.
The FlexAnalysis 3.4 software, a part of the FlexControl 3.4 suite, is a widely used tool for analyzing MALDI-MS data, and it can be integrated with sinapinic acid protocols to enhance the research process.
Furthermore, ferulic acid and α-cyano-4-hydroxycinnamic acid are structural analogues of sinapinic acid, and they share some of its properties and applications.
Researchers can explore the synergies and differences between these related compounds to expand their understanding and optimize their research strategies.
By leveraging the insights and capabilities provided by PubCompare.ai's AI-driven platform, scientists and researchers can streamline their workflows, access a wealth of information, and make more informed decisions when working with sinapinic acid and related compounds.
This integrated approach can lead to enhanced research outcomes, accelerated discoveries, and advancements in various fields, from analytical chemistry to pharmaceutical development.