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Sitosterol

Sitosterol is a plant sterol that plays a crucial role in regulating cholesterol levels and promoting cardiovascular health.
It is found naturally in various plant-based foods, such as vegetable oils, nuts, and legumes.
Sitosterol has been shown to inhibit the absorption of dietary cholesterol, potentially lowering the risk of heart disease.
Researchers utilize Sitosterol in a variety of studies, examining its effects on lipid profiles, inflammation, and overall metabolic function.
PubCompare.ai can enhance the efficiency and accuracy of Sitosterol research by providing access to a comprehensive database of protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best approaches and products for your studies.
Improve your research confidence and productivity with PubCompare.ai.

Most cited protocols related to «Sitosterol»

1
Inhibition of Acetylcholinesterase and Antioxidant Activity
Cited 11 times
AChE from Electrophorus electricus (CAS: 9000-81-1) was obtained from Sigma Aldrich, St. Loius, MO, United States, while BChE was derived from equine serum (9001-08-5) and was purchased from Sigma–Aldrich GmbH, Germany. Acetylthiocholine iodide (CAS1866-15-5) and butyrylthiocholine iodide (CAS 2494-56-6) were purchased from Sigma–Aldrich United Kingdom and Sigma–Aldrich Switzerland respectively. 5,5-Dithio-bis-nitrobenzoic acid (DTNB) (CAS 69-78-3) (Sigma–Aldrich GmbH, Germany) and galanthamine HBr Lycoris Sp. (CAS: 1953-04-4) (Sigma–Aldrich, France) were used in enzymes studies. Antioxidant reagents including DPPH (CAS: 1898-66-4) ABTS (CAS: 30931-67-0) were purchased from Sigma Aldrich St. Loius, MO, United States. H2O2 (batch no: A040) was obtained from Rehmat pharma Lahore, Pakistan. Potassium peroxodisulfate (LOT NO: 51240) was obtained from Labor chemikalien GmbH & Co KGD-30926 Seelze. For genotyping of transgenic animals, GF-1 tissue DNA extraction kit (Cat:GF-TD-100, Vivantis), agarose (Invitrogen CAT:75510-011, Carlsbad, CA, United States), boric acid (Serva CAT 15165, Germany), DNA Ladder (Serva CAT:15165, Germany), EDTA (Invitrogen CAT:75576-028, Carlsbad, CA, United States), ethanol (Merck CAT:26225745, Germany), ethidium bromide (Sigma CAT:E7637, United States), MgCl2 (Invitrogen CAT:AM9530G, Carlsbad, CA, United States), DNTPs (Promega CAT:U1515, United States), Taq polymerase (Thermo Scientific CAT: EP0402, United States), PCR primers (Thermo Scientific CAT:OIMR3610 F, OIMR3611 R), PCR grade distilled water (Thermo Scientific CAT: R0581), sucrose (Invitrogen CAT: 15503-022, Carlsbad, CA, United States), Tris EDTA solution (50X), 2XPCR Master mix (Fermentas CAT: K0171, EU), NaCl (Invitrogen CAT: 24740-011, Carlsbad, CA, United States) and tris (Invitrogen CAT: 15504-020, Carlsbad, CA, United States) were purchased from authorized dealers in Pakistan. Solvents and buffer salts used were of extra pure quality.
Ayaz M., Junaid M., Ullah F., Subhan F., Sadiq A., Ali G., Ovais M., Shahid M., Ahmad A., Wadood A., El-Shazly M., Ahmad N, & Ahmad S. (2017). Anti-Alzheimer’s Studies on β-Sitosterol Isolated from Polygonum hydropiper L.. Frontiers in Pharmacology, 8, 697.
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Publication 2017
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid acetylthiocholine iodide Animals, Transgenic Antioxidants boric acid Buffers Butyrylthiocholine Edetic Acid Electric Eel Enzymes Equus caballus Ethanol Ethidium Bromide Galanthamine Hydrobromide Iodides Lycoris Magnesium Chloride Nitrobenzoic Acids Obstetric Labor Oligonucleotide Primers Pain Peroxide, Hydrogen potassium persulfate Promega Salts Sepharose Serum Sodium Chloride Solvents Sucrose Taq Polymerase Tissues Tromethamine
2
Cholesterol Absorption and Metabolism in Mice
Cited 10 times

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Publication 2011
Acetate Anabolism Apolipoproteins B Apolipoproteins E Cholesterol Corn oil Feces Genotype High Density Lipoproteins Intestines, Small Lipids Liver Mice, House Phenobarbital Plasma Radioactivity sitosterol Sterols Tissues
3
Isolation and Evaluation of Anti-Alzheimer Compounds from P. hydropiper
Cited 9 times
In the search for new anti-Alzheimer’s and neuroprotective drugs from Polygonacae, P. hydropiper L. was identified, collected and processed for fractionation as previously reported from our laboratory (Ayaz et al., 2014b (link), 2016 (link)). A plant specimen was deposited with voucher no H.UOM.BG.107 at the herbarium of the University of Malakand, Chakdara, Dir (L), KP, Pakistan. Solvent based fractions and saponins were initially subjected to in vitro anti-Alzheimer’s studies. The solvent fractions, especially chloroform and ethyl acetate were selected for the isolation of pure compounds due to their prominent activities in preliminary assays. Several compounds were isolated using gravity column chromatography eluting with n-haxane and ethyl acetate. The purified compounds were rotary evaporated to remove any remaining solvent. Initially, 1H NMR spectra was obtained to reveal the chemical structure by comparing the spectra with those reported in the literature. The 13C NMR spectra were used to ascertain the carbon skeleton of the compounds. The spectral data was supplemented by using mass spectrometry to confirm the structures. Among the isolated compounds, β-sitosterol was most active in the preliminary analysis and was evaluated in detail (Figure 1).
Ayaz M., Junaid M., Ullah F., Subhan F., Sadiq A., Ali G., Ovais M., Shahid M., Ahmad A., Wadood A., El-Shazly M., Ahmad N, & Ahmad S. (2017). Anti-Alzheimer’s Studies on β-Sitosterol Isolated from Polygonum hydropiper L.. Frontiers in Pharmacology, 8, 697.
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Publication 2017
1H NMR antibiotic H107 Biological Assay Carbon Carbon-13 Magnetic Resonance Spectroscopy Chloroform Chromatography ethyl acetate Fractionation, Chemical Gravity Mass Spectrometry Neuroprotective Agents Plants Saponins sitosterol Skeleton Solvents
4
Cholesterol Kinetics in ApoE-Deficient Mice
Cited 7 times

Protocol full text hidden due to copyright restrictions

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Publication 2011
Acetate Anabolism Apolipoproteins B Apolipoproteins E Cholesterol Corn oil Feces Genotype High Density Lipoproteins Intestines, Small Lipids Liver Mice, House Phenobarbital Plasma Radioactivity sitosterol Sterols Tissues
5
Optimized HPLC Analysis of Triterpenoids
Cited 6 times
The optimized chromatographic analysis was carried out using a Waters Alliance 2695 liquid chromatography system (Waters, Milford, CT, USA) equipped with 2996 photodiode array detector (Waters, Milford, CT, USA) and ACE C18 (150 × 4.6 mm, 3 μm) column (ACT, Aberdeen, UK). Since all triterpenoids cannot be separated in a single chromatographic run, different chromatographic conditions were used.
For the analysis of triterpenoid acids (maslinic, corosolic, betulinic, oleanolic, ursolic acids) and neutral triterpenoids with chromophores (betulin, erythrodiol, uvaol), the mobile phase consisted of acetonitrile and water (89:11, v/v), delivered at a flow rate of 0.7 mL/min in the isocratic mode. The column temperature was set at 20 °C with an injection volume of 10 μL. Whereas the isocratic elution system for the analysis of neutral triterpenoids, which lacks chromophores (lupeol, β-amyrin, α-amyrin, friedelin) and phytosterol (β-sitosterol), consisted of acetonitrile and methanol (10:90, v/v). The column temperature was set at 35 °C, the flow rate was 1 mL/min, and the sample injection volume was 10 μL.
Detection of all triterpenoids was performed at a wavelength of 205 nm corresponding to the maximum absorption, and peaks were identified with retention times as compared with standards.
Vilkickyte G, & Raudone L. (2021). Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.. Molecules, 26(6), 1645.
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Publication 2021
acetonitrile Acids betulin Chromatography erythrodiol friedelin Liquid Chromatography lupeol Methanol Oleanolic Acid Phytosterols Retention (Psychology) sitosterol Triterpenes ursolic acid uvaol

Most recents protocols related to «Sitosterol»

1
Quantification of β-Sitosterol in Javanese Ginseng Roots
Not available on PMC !
In this analysis, 10 mg of b-sitosterol standard was dissolved in a few drops of PA-grade chloroform and then supplemented with HPLC-grade methanol to reach a final volume of 10 mL. This solution was then prepared as a stock solution at a concentration of 1000 ppm, and further dilutions were made as needed using HPLCgrade methanol until the desired concentrations were achieved. All solutions with varying concentrations were filtered through a 0.22 µm PTFE filter before being injected into the HPLC system. HPLC analysis was performed at a flow rate of 1 mL/minute, using a mobile phase composed of methanol:acetonitrile (9:1, v/v), C18 stationary phase, and a wavelength of 202 nm to detect b-sitosterol (Khonsa et al., 2022) (link). After obtaining the peak areas from the HPLC analysis, a graph was constructed by plotting the concentrations of the standard b-sitosterol solutions (x-axis) against the corresponding peak areas (y-axis). The resulting line equation became the standard curve for b-sitosterol.
For the analysis of b-sitosterol content in the dry extract of Javanese ginseng roots, the extract was initially dissolved in 5 mL HPLC-grade methanol. Subsequently, the extract solution was filtered through a 0.22 µm PTFE filter into an HPLC vial, reaching a final volume of 1.5 mL. This solution was then injected into the HPLC instrument using the same HPLC settings as those used for the analysis of standard b-sitosterol solutions of various concentrations. The peak area obtained from HPLC analysis was used as the y-value in the standard curve equation to determine the concentration of bsitosterol in the sample as the x-value.
The concentration of b-sitosterol was then converted into the b-sitosterol content in the root extract using Eq. ( 5) (Fuentes-Arderiu, 2013). where C(SIT) represents the concentration of b-sitosterol, Vs is the solvent volume (HPLC-grade methanol), and RPW is the weight of root powder used for one-sample replication. Furthermore, the bsitosterol productivity of the roots was calculated using Eq. ( 6) (Mangoli, 2020) . In calculating the b-sitosterol productivity from the roots, we assumed the dimensions of the hydroponic gully to be 2.1 m x 0.58 m (length x width) with a total of 48 plants in each hydroponic set.
Widodo S.O.W., Faizal A., & Melani L. (2024). The influence of chitosan on plant growth and -sitosterol content in adventitious roots of Talinum paniculatum in NFT hydroponic cultivation system. Current Research on Biosciences and Biotechnology, 5(2), 5-11.
Publication 2024
2
Molecular Docking of β-Sitosterol Targets
A molecular docking approach was used to study the relationship between β-sitosterol and its main targets. Protein Data Bank (PDB; http://www.rcsb.org/pdb/home/home.do) was used to collect structural data of the key core genes and PubChem (https://pubchem.ncbi.nlm.nih.gov) was used to obtain chemical data of β-sitosterol. To simulate how β-sitosterol binds to the target protein, PyMol software was applied to dehydrate, hydrogenate, and charge. AutoDockTools-1.5.6 software was used to determine the location and size of the Grid box, the binding site of the disease target protein to the ligand. Autodock vina V1.1.2 software was used to complete the molecular docking and assess the affinity of the compounds to the target proteins. PyMOL software was used for visualization and numerical heat map analysis.
Zhang Z., Shang B., Mao X., Shi Y., Zhang G, & Wang D. (2024). Prognostic Risk Models Using Epithelial Cells Identify β-Sitosterol as a Potential Therapeutic Target Against Esophageal Squamous Cell Carcinoma. International Journal of General Medicine, 17, 1193-1211.
Publication 2024
3
Quantification of β-Sitosterol in Pet-B
β-sitosterol was chosen as a marker for the determination of Pet-B as it is isolated in considerable amount from Pet-B together with its various biological activities viz. wound healing, anti-oxidant, anti-inflammatory or anti-microbial activities. It was determined by HPTLC (CAMAG) in Pet-B according to Mallick and Dighe (2014) [41 (link)].
Abdel Halim M.B., Eid H.H., El Deeb K.S., Metwally G.F., Masoud M.A., Ahmed-Farid O.A, & El Messiry H.M. (2024). The study of wound healing activity of Thespesia populnea L. bark, an approach for accelerating healing through nanoparticles and isolation of main active constituents. BMC Complementary Medicine and Therapies, 24, 85.
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Publication 2024
4
Zebrafish Models for NAFLD Study
Formulation of larval zebrafish HFD: Following the dissolution of 10 g of egg yolk powder and 1 g of cholesterol, the resulting mixture was homogenized on a magnetic stirrer and subsequently dried overnight in a freeze dryer (SCIENTE, Ningbo, China).
Formulation of adult zebrafish HFD: After the dissolution of 10 g of egg yolk powder, 10 g of cholesterol, and 3 g of glucose, the resulting mixture was homogenized with 100 g of zebrafish feed using a magnetic stirrer. Subsequently, the mixture was dried overnight in a freeze-dryer (SCIENTE, Ningbo, China). The HFD for the experimental group was enriched with 50 mg of β-sitosterol, while the control group received an equivalent amount of DMSO as the β-sitosterol group.
Construction of a larval zebrafish model of NAFLD: Fish were raised to 5 days post-fertilization (dpf) in an E3 medium, with 0.003% 1-phenyl-2-thiourea (PTU) added at 22 h post-fertilization (hpf) to clear pigment [33 (link)]. Zebrafish at 5 dpf were selected for the experiment, and the larvae were fed with HFD twice a day for 5 days. During feeding, 3% glucose solution was added for soaking, and the feeding time was 1 h. After feeding, a fresh E3 culture medium was replaced. The experimental group was given 200 μg/mL of β-sitosterol, the positive control group received 62.5 μg/mL of bezafibrate (a peroxisome proliferator-activated receptor activator that reduces blood lipids), and the control group received the same amount of DMSO as the β-sitosterol group. Subsequently, the accumulation of triglyceride and cholesterol in zebrafish larvae was detected [29 (link)]. Toxicity tests were used to determine bezafibrate and β-sitosterol concentrations.
Construction of an adult zebrafish model of NAFLD: This model was established by a feeding regimen of HFD administered twice daily for one month. Subsequently, the liver of the zebrafish was collected to determine the levels of cholesterol and triglyceride, followed by statistical analysis to confirm the establishment of the model [34 (link)].
Zhang P., Liu N., Xue M., Zhang M., Xiao Z., Xu C., Fan Y., Qiu J., Zhang Q, & Zhou Y. (2024). β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model. Animals : an Open Access Journal from MDPI, 14(9), 1289.
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Publication 2024
5
Optimizing Chitosan Effects on Plant Growth
Not available on PMC !
The research design in this study used a completely randomized design (CRD) with four variations of chitosan concentrations: 0, 12.5, 25, and 50 ppm, along with 12 replications (for cultivation data) or 3 replications (for HPLC data). ANOVA (Analysis of Variance) One-Way test was conducted to determine the presence of differences among the data groups. If differences were found, ANOVA was followed by Tukey's test (at a significance level of 0.05) to assess the significance of the effects of chitosan concentration on variables such as root weight increase, shoot weight increase, growth rate, root-to-shoot ratio, b-sitosterol content in the roots, and b-sitosterol productivity from the roots. All statistical analyses in this research were conducted using Minitab 19 software.
Widodo S.O.W., Faizal A., & Melani L. (2024). The influence of chitosan on plant growth and -sitosterol content in adventitious roots of Talinum paniculatum in NFT hydroponic cultivation system. Current Research on Biosciences and Biotechnology, 5(2), 5-11.
Publication 2024

Top products related to «Sitosterol»

1
β sitosterol by Merck Group
Sourced in United States, Germany, China, Australia, Italy, France, Japan
β-sitosterol is a phytosterol compound commonly found in plants. It is a naturally occurring sterol that is structurally similar to cholesterol. β-sitosterol is often used as a reference standard in analytical applications.
2
Stigmasterol by Merck Group
Sourced in United States, Germany, China, Australia, Spain, United Kingdom, Italy, France
Stigmasterol is a plant-derived sterol compound commonly used as a reference standard and analytical tool in laboratory settings. It serves as a key component in various analytical techniques, such as chromatography and spectroscopy, to identify and quantify similar sterol compounds in samples. Stigmasterol's core function is to provide a reliable and well-characterized reference point for the analysis and identification of other sterols in research and testing applications.
3
Campesterol by Merck Group
Sourced in United States, Germany, China, Australia, Italy, France
Campesterol is a plant sterol compound found in various plant sources. It is a natural component that can be isolated and used as a reference standard or analytical tool in laboratory settings.
4
Cholesterol by Merck Group
Sourced in United States, Germany, United Kingdom, India, Japan, Sao Tome and Principe, China, France, Spain, Canada, Switzerland, Italy, Australia, Israel, Brazil, Belgium, Poland, Hungary, Macao
Cholesterol is a lab equipment product that measures the concentration of cholesterol in a given sample. It provides quantitative analysis of total cholesterol, HDL cholesterol, and LDL cholesterol levels.
5
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
6
Sitosterol by Merck Group
Sourced in United States, Germany
Sitosterol is a phytosterol compound derived from plant sources. It is used as a raw material in the manufacturing of various pharmaceutical and biochemical products. Sitosterol serves as a core ingredient in the production of steroids and other related compounds. Its primary function is to provide a natural source of sterols for further chemical processing and formulation development.
7
5α cholestane by Merck Group
Sourced in United States, Germany, China, Poland
5α-cholestane is a laboratory standard compound used as a reference material for analytical procedures. It is a naturally occurring steroid hydrocarbon found in various biological samples. 5α-cholestane serves as a marker compound for identification and quantification purposes in analytical techniques such as gas chromatography and mass spectrometry.
8
Lupeol by Merck Group
Sourced in United States, Germany
Lupeol is a lab equipment product manufactured by Merck Group. It is a naturally occurring triterpene alcohol compound found in various plants. Lupeol serves as a core component for further research and analysis purposes in scientific and laboratory settings.
9
Quercetin by Merck Group
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
10
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

More about "Sitosterol"

Sitosterol, a plant-derived sterol, plays a crucial role in regulating cholesterol levels and promoting cardiovascular health.
It is found naturally in various plant-based foods like vegetable oils, nuts, and legumes.
Sitosterol inhibits the absorption of dietary cholesterol, potentially lowering the risk of heart disease.
Researchers utilize Sitosterol, also known as β-sitosterol, in studies examining its effects on lipid profiles, inflammation, and overall metabolic function.
Stigmasterol and Campesterol are other plant sterols related to Sitosterol that share similar cholesterol-lowering properties.
Cholesterol, a steroid lipid, is essential for cell membrane structure and hormone production, but high levels can contribute to cardiovascular issues.
Methanol is a toxic alcohol that should be avoided, while Sitosterol, 5α-cholestane, and Lupeol are plant-derived compounds with potential health benefits.
Quercetin, a flavonoid compound, has been studied in conjunction with Sitosterol for its anti-inflammatory and antioxidant effects.
FBS, or fetal bovine serum, is a common cell culture supplement that may be used in Sitosterol research.
PubCompare.ai can enhance the efficiency and accuracy of Sitosterol research by providing access to a comprehensive database of protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best approaches and products for your studies.
Improve your research confidence and productivity with PubCompare.ai.