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Sodium Acetate Trihydrate

Sodium acetate trihydrate is a chemical compound with the formula CH3COONa·3H2O.
It is a crystalline solid that is commonly used in a variety of applications, including as a buffer in biochemical reactions, a food additive, and a de-icing agent.
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Most cited protocols related to «Sodium Acetate Trihydrate»

Sodium Acetate Trihydrate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and Tris(hydroxymethyl)aminomethane (Tris) were purchased from Thermo Fisher Scientific Inc. MES sodium salt was purchased from Sigma as an anhydrate. Bis-Tris (2,2-Bis(hydroxymethyl)-2,2’,2”-nitrilotriethanol) was purchased from Acros Organics, and Tricine (N-Tris[hydroxymethyl]methylglycine was purchased from BioRad.
Each universal buffer is an equimolar mixture of three reagents with each performing as a buffer when the pH is near its pKa. Universal buffers were prepared by adding appropriate amounts of individual dried buffer to distilled water. To standardize concentration for each universal buffer used in the titration and temperature experiments, the sum concentration of reagents was 60mM for each formulation. For the titrations, pH of the individual buffer and universal buffers was set to pH 11 using 10M sodium hydroxide and then brought to final concentration by addition of water. Titrations from high to low pH were conducted by step-wise addition of 5M hydrochloric acid followed by vigorous mixing. pH was measured using a standard pH electrode (Thermo Fisher Scientific Dj Glass AG/AGCL Ph Electrode with a waterproof BNC).
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Publication 2015
Acids Bistris Buffers HEPES Hydrochloric acid methylamine Sarcosine Sodium Sodium Acetate Trihydrate Sodium Chloride Sodium Hydroxide Titrimetry tricine triethanolamine Tromethamine

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Publication 2016
Amniotic Fluid ATP8A2 protein, human Baculoviridae Chromatography, Affinity Cloning Vectors Crystallization Cytokinesis Diffusion DNA, Complementary Endosomes Escherichia coli Gel Chromatography Glycerin Glycosylated Proteins HEK293 Cells his6 tag Inclusion Bodies Metals Molar Mothers Mucins Mutagenesis, Site-Directed Niemann-Pick C1 Protein Niemann-Pick Disease, Type C1 Peptide Hydrolases Polyethylene Glycols Proteins Sf9 Cells Sodium Acetate Trihydrate Sodium Chloride Strains Thermolysin

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Publication 2009
Acetate Acetic Acid alum, potassium anti-IgG anti-IgM Antibodies Avidin Biotin Buffers Cloning Vectors Ethanol Exhaling Goat imidazole imidazole acetate Immunoglobulins Light Microscopy Mice, House Microscopy Peroxide, Hydrogen Pharmaceutical Preparations Phosphates Saline Solution Serum Sodium Acetate Trihydrate sodium metaperiodate Tissues Triton X-100 Xylene
RiVax was obtained from a 100 l Escherichia coli fermentation and purified essentially as described by Smallshaw and coworkers (Peek et al., 2007 ▶ ; Smallshaw et al., 2002 ▶ ). The protein was stored as a stock at 253 K in 10 mM histidine pH 6.0, 140 mM sodium chloride and 50% glycerol (a stabilizing excipient). The glycerol was removed by dialysis against 4 l 75 mM Tris–HCl pH 8.0, 1 mM EDTA at 277 K. Dithiothreitol was added to a final concentration of 5 mM using a 1.0 M stock. Crystals were grown by the hanging-drop method using a 1:1 ratio of protein (2.8 mg ml−1) to precipitant. The precipitant used was 30% ammonium sulfate containing 50 mM sodium acetate pH 4.2. Crystals appeared within 24 h. Crystals were soaked in Crystal Screen Cryo (Hampton Research, Aliso Viejo, California, USA) solution No. 20 [0.16 M ammonium sulfate, 0.08 M sodium acetate trihydrate pH 5.0, 20%(w/v) PEG 4000, 20%(w/v) glycerol] and flash-frozen in liquid nitrogen. Diffraction data were collected with a Bruker FR591 high-flux rotating-anode X-ray diffractometer (PROTEUM) and a SMART 6000 2K CCD detector. Initial phases were calculated using the structure of RTA. The structure was solved by molecular replacement using AMoRe (Navaza, 2001 ▶ ). Simulated annealing was carried out with CNS v.1.1 (Brünger et al., 1998 ▶ ). The model was refined using REFMAC5 (Murshudov et al., 2011 ▶ ) and model building and solvent addition was performed with Coot (Emsley & Cowtan, 2004 ▶ ).
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Publication 2011
Dialysis Dithiothreitol Edetic Acid Escherichia coli Excipients Fermentation Freezing Glycerin Histidine Nitrogen polyetheretherketone Proteins Radiography RiVax Sodium Acetate Sodium Acetate Trihydrate Sodium Chloride Solvents Sulfate, Ammonium Tromethamine
Constructs of T. maritima Tm14S with altered termini (residues 107–192, 107–193, 107–194,106–191, 106–192) compared to those that produced the previous 3UR1 structure (107–191) were PCR cloned into vector pET28a (Novagen) and expressed with an N-terminal Histidine6 tag in E. coli strain BL21 (RIL DE3) (Novagen) after induction with IPTG at 18°C and overnight growth for 21 hours. The Tm14s fragments were purified first with Ni-NTA chromatography, followed by overnight thrombin digestion, and then size-exclusion chromatography (Superdex 75 Hi-load FPLC column in 50 mM NaCl, 100 mM Tris pH 7.5, 10% glycerol). T. maritima CheW and CheA Δ354 (P4P5 domain, residues 355–671) were expressed and purified as described previously34 (link).
Cubic shaped crystals (50×50×50 μm3) were grown from a mixture of 520 μM Tm14s, 457 μM CheA Δ354 and 121 μM CheW after 1 month by vapor diffusion from a 2 μl drop (1:1 mixture of protein and reservoir: 500 μl reservoir of 0.2 M sodium acetate trihydrate, 0.1 M Tris (pH 8.5), 15% w/v polyethylene glycol 4,000). Crystals with a similar shape and size as those derived from Tm14S (residues 107–191) were grown after 1 month. The new crystals (from Tm14S residues 107–192) consistently diffracted to 3.5 Å resolution. Crystals were soaked briefly in cryoprotectant that consisted of 85/15% (v/v) reservoir solution with glycerol prior to data collection in a N2 cold stream. Diffraction data were collected at 100K with synchrotron radiation at beamline A1 at the Cornell High Energy Synchrotron Source (CHESS). Selenomethionine was also incorporated into the Tm14S (residues 107–192) to aid in efforts to determine the helical registry, but unfortunately, the selenomethionine incorporated protein did not produce crystals.
Publication 2013
Chewing Chromatography Cloning Vectors Cold Temperature Cryoprotective Agents Cuboid Bone Diffusion Digestion Escherichia coli Gel Chromatography Glycerin Helix (Snails) Isopropyl Thiogalactoside Polyethylene Glycols Proteins Radiation Selenomethionine Sodium Acetate Trihydrate Sodium Chloride Strains Thrombin Tromethamine

Most recents protocols related to «Sodium Acetate Trihydrate»

Fabs were crystallized by the hanging drop method. Crystals of unbound UCA Fab with the Y35N (LC) mutation and unbound I-2 Fab with H35N (HC) and Y35N (LC) mutations were grown over solutions of 0.1 M succinic acid (pH 7), 0.1 M bicine (pH 8.5), and 30% polyethylene glycol monomethyl ether 550 or 0.8 M lithium sulfate monohydrate, 0.1 M sodium acetate trihydrate (pH 4), and 4% polyethylene glycol 200 (Hampton Research, #HR2-084), respectively, in a 96-well plate (Greiner, #655101) with ViewDrop II plate seals (sptlabtech, #4150-05600). Crystals were apparent after ~5–7 days. Then, 1 μL of 12% (+/-)-2-methyl-2,4-pentanediol (MPD) in the corresponding solution was added for cryoprotection. The crystals were then harvested and flash-cooled in liquid nitrogen.
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Publication 2023
lithium sulfate monomethoxypolyethylene glycol Mutation N,N-bis(2-hydroxyethyl)glycine Nitrogen Phocidae Polyethylene Glycols Sodium Acetate Trihydrate Succinic Acid

Nebivolol HCl (NEB, 99.4% purity), was kindly supplied by Sigma Company for Pharmaceutical Industries, Quesna, Egypt.

Telmisartan (TEL,99.91% purity) was gently given by International Drug Agency for Pharmaceutical Industry (IDI), Cairo, Egypt

Pharmaceutical Preparations

Nevilob Tablets (Batch No. 2033518) labeled to include 5 mg NEB per tablet, a product of Marcyrl Pharmaceutical Industries, Cairo, Egypt.

Micardis Tablets (Batch No. 906949) are labeled to include 40 mg TEL per tablet, a product of Boehringer Ingelheim Pharma, Germany.

Both preparations were purchased from a local Pharmacy in the Egyptian market.

Methanol, acetonitrile, ethanol, Phenol, hexane, Tween-80, 99% cetrimide, 95% sodium dodecyl sulphate, methylcellulose, and β-cyclodextrin were bought from Sigma-Aldrich, Germany. All surfactants were prepared in distilled water at a concentration of 1% w/v or v/v.

Also, 96%acetic acid, sodium acetate trihydrate, sodium hydroxide, and boric acid were bought from the same source.

Acetate buffer (0.2 M) was prepared using sodium acetate trihydrate and acetic acid and its pH was adjusted at 3.7 − 5.5, while borate buffer (0.2 M) was composed of boric acid and potassium chloride, and pH was adjusted to cover the range of (6–9) using sodium hydroxide.

Human plasma samples were obtained from Blood Bank, Mansoura University Hospital (Mansoura, Egypt), and kept frozen at − 20 °C until use after gentle thawing.

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Publication 2023
Acetate Acetic Acid acetonitrile Borates boric acid Buffers Cetrimide Cyclodextrins Ethanol Freezing Hexanes Homo sapiens Methanol Methylcellulose Micardis Nebivolol Pharmaceutical Preparations Phenol Plasma Potassium Chloride Sodium Acetate Trihydrate Sodium Hydroxide Sulfate, Sodium Dodecyl Surfactants Tablet Telmisartan Tween 80
Cultures were routinely grown on Lysogeny Broth (LB) during plasmid and strain construction, while aceE mutants were grown on TYA medium containing (per L) 10 g tryptone, 5 g NaCl, 1 g yeast extract, and 1 g sodium acetate trihydrate [32 ]. As needed, antibiotics were included in medium (final concentration): ampicillin (100 µg/ml), kanamycin (40 µg/ml), and chloramphenicol (20 µg/ml). For counter‐selection against sacB, the medium was supplemented with 100 g/L sucrose, and NaCl was excluded.
The defined basal medium to which carbon/energy sources were added contained (per L): 8 g NH4Cl, 1.2 g KH2PO4, 1.0 K2HPO4, 2.0 g K2SO4, 0.6 g MgSO4·7H2O, 0.25 mg ZnSO4·7H2O, 0.125 mg CuCl2·2H2O, 1.25 mg MnSO4·H2O, 0.875 mg CoCl2·6H2O, 0.06 mg H3BO3, 0.25 mg Na2MoO4·2H2O, 5.5 mg FeSO4·7H2O, 20 mg Na2EDTA·2H2O, 20 mg citric acid, 20 mg thiamine·HCl. In shake flask cultures used 20.9 g 3‐[N‐morpholino]propanesulfonic acid (100 mM MOPS) while for batch processes used 25 mM MOPS. Thiamine was filtered sterilized, and other medium components were autoclaved in compatible mixtures, combined and then adjusted to a pH of 7.1 with 20% (w/v) NaOH.
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Publication 2023
Acids Ampicillin Antibiotics, Antitubercular Batch Cell Culture Techniques Carbon Chloramphenicol Citric Acid cupric chloride Kanamycin Lysogeny morpholinopropane sulfonic acid Morpholinos Plasmids potassium phosphate, dibasic Sodium Acetate Trihydrate Sodium Chloride sodium molybdate(VI) Sucrose Sulfate, Magnesium Thiamine thiamine hydrochloride Yeast, Dried
The ultrapure water was prepared by Milli-Q Ultrapure Water System (Millipore Corporation, Billerica, MA, USA). GABA, L-glutamic acid, L-alanine, L-serine, and L-lysine were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Ninhydrin was purchased from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Yeast extract was purchased from Angel Yeast Co., Ltd. (Wuhan, China). The reagents used for HPLC were HPLC-grade. All other chemicals were of analytical grade. L. brevis CD0817 is a previously identified GABA producer [6 (link),33 (link)].
The 0.8 M sodium acetate buffer (pH 5.8) was made by dissolving 109.2 g sodium acetate trihydrate and 40.0 mL acetic acid (1 M) to 0.8 L ultrapure water, followed by adding ultrapure water to a final volume of 1 L. The 0.2 M sodium acetate buffer (pH 5.0) contained 17.2 g sodium acetate trihydrate and 73.0 mL acetic acid (1 M). An aliquot of 2.9 mL SINICS comprised 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and a 35 μL 0.2 M sodium acetate buffer (pH 5.0). The 0.1 M solutions of L-glutamate acid, GABA, L-alanine, L-serine, and L-lysine were individually prepared using ultrapure water, and diluted to 10 mM as required. The borate buffer was manufactured by adding 4.9 g boric acid into 100.0 mL of water, adjusting the pH to 10.4 with NaOH, and then diluting it to 200.0 mL. The amino acids derivatization reagent was made by dissolving 10 mg o-phthalaldehyde in 2.5 mL acetonitrile, then supplementing 10 μL β-mercaptoethanol. The mobile phase of HPLC consisted of solvent A and solvent B at a volume ratio of 4:1. Solvent A was prepared by mixing 9.8 mL of 20.0 mM sodium acetate buffer (pH 7.3) and 0.2 mL triethylamine. Solvent B was acetonitrile.
The seed medium (pH 5.0) for L. brevis CD0817 consisted of (g/L): yeast extract, 35.0; MnSO4·H2O, 0.05; monosodium L-glutamate, 28.0; tween-80, 1.0; and glucose, 10.0. The fermentation medium was (g/L): yeast extract, 35.0; MnSO4·H2O, 0.05; tween-80, 1.0; glucose, 5.0; monosodium L-glutamate, 33.8; and 650.0 g solid powder L-glutamic acid was added prior to inoculation. Glucose, L-glutamic acid/monosodium L-glutamate, and the other components were individually sterilized at 121 °C for 20 min and mixed together before use.
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Publication 2023
2-Mercaptoethanol Acetic Acid acetonitrile Acids Alanine Amino Acids Borates boric acid Buffers Ethanol ethyl acetate Fermentation gamma Aminobutyric Acid Glucose Glutamates Glutamic Acid High-Performance Liquid Chromatographies Lysine Ninhydrin o-Phthalaldehyde Powder Serine Sodium Acetate Sodium Acetate Trihydrate Sodium Glutamate Solvents triethylamine Tween 80 Vaccination Yeast, Dried
L-Phenylalanine was obtained from Fluka (Buchs, Switzerland). N,N-dimethylformamide (DMF), sodium acetate trihydrate, trifluoroacetic acid (TFA), dichloromethane (DCM), 2,5-dihydroxy-benzoic acid (DHB) and N-methyl morpholine (NMM) were purchased from Sigma-Aldrich (Steinheim, Germany). Rink amide resin (50–90 mesh, 0.51 mmol g−1) purchased from Sigma-Aldrich was used as a solid support. Acetic acid glacial and the amino acids protected at N-terminal with Fmoc group (9-fluorenylmethyloxycarbonyl) used in solid phase synthesis were purchased from Merck (Darmstadt, Germany) and piperazine—N,N′-bis (2-ethanesulfonic acid), PIPES buffer was from Carl Roth (Karlsruhe, Germany). Benzotriazol-1-yl-oxy-trispyrrolidinophosphonium-hexafluoro-phosphate (PyBOP) used as activator was purchased from NovaBiochem (Novabiochem, Merck KGaA, Darmstadt, Germany). Tris base (ULTROL®Grade) was obtained from the Calbiochem (EMD Chemicals, Inc., San Diego, CA, USA) and Thioflavin T from EMD (Millipore, Bedford, MA, USA). Acetonitrile (ACN) HPLC grade and piperidine were purchased from Merck (Darmstadt, Germany). All the solutions were prepared using deionized water (18.2 MΩ∙cm) produced by a Milli-Q system (Millipore, Bedford, MA, USA). All other reagents were used without further purification.
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Publication 2023
4-methylmorpholine Acetic Acid acetonitrile Amino Acids Benzoic Acid Buffers Dimethylformamide ethane sulfonate High-Performance Liquid Chromatographies Methylene Chloride Phenylalanine Phosphates Piperazine piperazine-N,N'-bis(2-ethanesulfonic acid) piperidine Rink amide resin Sodium Acetate Trihydrate thioflavin T Trifluoroacetic Acid Tromethamine

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Sodium acetate trihydrate is a chemical compound commonly used as a buffer and pH adjuster in various laboratory applications. It is a white, crystalline solid that dissolves readily in water. Sodium acetate trihydrate is widely utilized in biochemical, analytical, and chemical research due to its ability to maintain a stable pH environment.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Acetic acid is a colorless, vinegar-like liquid chemical compound. It is a commonly used laboratory reagent with the molecular formula CH3COOH. Acetic acid serves as a solvent, a pH adjuster, and a reactant in various chemical processes.
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DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.
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The Folin-Ciocalteu reagent is a colorimetric reagent used for the quantitative determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that undergo a color change when reduced by phenolic compounds.
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Sodium carbonate is a water-soluble inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a pH regulator, water softener, and cleaning agent in various industrial and laboratory applications.
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Glacial acetic acid is a colorless, odorous, and corrosive liquid used as a laboratory reagent. It has a chemical formula of CH3COOH and a concentration of 99.7% or higher. Glacial acetic acid is commonly used in various analytical and research applications, serving as a solvent, catalyst, and pH modifier.

More about "Sodium Acetate Trihydrate"

Sodium acetate trihydrate (CH3COONa·3H2O) is a versatile chemical compound with a wide range of applications.
Also known as sodium acetate, this crystalline solid is commonly used as a buffer in biochemical reactions, a food additive, and a de-icing agent.
The compound is composed of sodium (Na), acetate (CH3COO-), and three water molecules (3H2O), forming a unique crystal structure.
Sodium acetate trihydrate is known for its ability to regulate pH levels and maintain a stable environment in various applications.
In research and laboratory settings, sodium acetate trihydrate is often used as a buffer to control the acidity or basicity (pH) of solutions.
This is particularly important in biochemical reactions, such as those involving enzymes, proteins, or other biomolecules, where the pH needs to be carefully maintained for optimal performance.
Aside from its use in biochemistry, sodium acetate trihydrate finds applications in the food industry as a preservative, flavoring agent, and pH regulator.
It is commonly used in the production of pickles, sauces, and other food products to enhance flavor and extend shelf life.
Furthermore, sodium acetate trihydrate has been employed as a de-icing agent, particularly in cold climates, due to its ability to lower the freezing point of water.
This property makes it useful for clearing snow and ice from roads, walkways, and other surfaces.
When working with sodium acetate trihydrate, researchers can benefit from the AI-powered platform provided by PubCompare.ai.
This platform allows seamless access to protocols from literature, preprints, and patents, enabling researchers to identify the optimal protocols and products for their experiments.
By utilizing cutting-edge AI comparisons, researchers can enhance the reproducibility of their work and achieve better results.
In addition to sodium acetate trihydrate, related compounds such as gallic acid, hydrochloric acid, sodium hydroxide, methanol, acetic acid, DPPH, Folin-Ciocalteu reagent, and sodium carbonate may also be relevant in various research and industrial applications.
Exploring the properties and interactions of these substances can provide valuable insights and opportunities for innovation.
Start optimizing your experiments today with PubCompare.ai and unlock the full potential of sodium acetate trihydrate and other related compounds in your research endeavors.