Each universal buffer is an equimolar mixture of three reagents with each performing as a buffer when the pH is near its pKa. Universal buffers were prepared by adding appropriate amounts of individual dried buffer to distilled water. To standardize concentration for each universal buffer used in the titration and temperature experiments, the sum concentration of reagents was 60mM for each formulation. For the titrations, pH of the individual buffer and universal buffers was set to pH 11 using 10M sodium hydroxide and then brought to final concentration by addition of water. Titrations from high to low pH were conducted by step-wise addition of 5M hydrochloric acid followed by vigorous mixing. pH was measured using a standard pH electrode (Thermo Fisher Scientific Dj Glass AG/AGCL Ph Electrode with a waterproof BNC).
Sodium Acetate Trihydrate
It is a crystalline solid that is commonly used in a variety of applications, including as a buffer in biochemical reactions, a food additive, and a de-icing agent.
PubCompare.ai's AI-powered platform allows researchers to sealmessly locate protocols from literature, preprints, and patents, and utilize cutting-edge AI comparisons to identify the optimal protocols and products for working with sodium acetate trihydrate.
This can help enhance research reproducibility and achieve better results.
Start optimizing your experiments today with PubCompare.ai.
Most cited protocols related to «Sodium Acetate Trihydrate»
Each universal buffer is an equimolar mixture of three reagents with each performing as a buffer when the pH is near its pKa. Universal buffers were prepared by adding appropriate amounts of individual dried buffer to distilled water. To standardize concentration for each universal buffer used in the titration and temperature experiments, the sum concentration of reagents was 60mM for each formulation. For the titrations, pH of the individual buffer and universal buffers was set to pH 11 using 10M sodium hydroxide and then brought to final concentration by addition of water. Titrations from high to low pH were conducted by step-wise addition of 5M hydrochloric acid followed by vigorous mixing. pH was measured using a standard pH electrode (Thermo Fisher Scientific Dj Glass AG/AGCL Ph Electrode with a waterproof BNC).
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
Most recents protocols related to «Sodium Acetate Trihydrate»
Nebivolol HCl (NEB, 99.4% purity), was kindly supplied by Sigma Company for Pharmaceutical Industries, Quesna, Egypt.
Telmisartan (TEL,99.91% purity) was gently given by International Drug Agency for Pharmaceutical Industry (IDI), Cairo, Egypt
Nevilob Tablets (Batch No. 2033518) labeled to include 5 mg NEB per tablet, a product of Marcyrl Pharmaceutical Industries, Cairo, Egypt.
Micardis Tablets (Batch No. 906949) are labeled to include 40 mg TEL per tablet, a product of Boehringer Ingelheim Pharma, Germany.
Both preparations were purchased from a local Pharmacy in the Egyptian market.
Methanol, acetonitrile, ethanol, Phenol, hexane, Tween-80, 99% cetrimide, 95% sodium dodecyl sulphate, methylcellulose, and β-cyclodextrin were bought from Sigma-Aldrich, Germany. All surfactants were prepared in distilled water at a concentration of 1% w/v or v/v.
Also, 96%acetic acid, sodium acetate trihydrate, sodium hydroxide, and boric acid were bought from the same source.
Acetate buffer (0.2 M) was prepared using sodium acetate trihydrate and acetic acid and its pH was adjusted at 3.7 − 5.5, while borate buffer (0.2 M) was composed of boric acid and potassium chloride, and pH was adjusted to cover the range of (6–9) using sodium hydroxide.
Human plasma samples were obtained from Blood Bank, Mansoura University Hospital (Mansoura, Egypt), and kept frozen at − 20 °C until use after gentle thawing.
The defined basal medium to which carbon/energy sources were added contained (per L): 8 g NH4Cl, 1.2 g KH2PO4, 1.0 K2HPO4, 2.0 g K2SO4, 0.6 g MgSO4·7H2O, 0.25 mg ZnSO4·7H2O, 0.125 mg CuCl2·2H2O, 1.25 mg MnSO4·H2O, 0.875 mg CoCl2·6H2O, 0.06 mg H3BO3, 0.25 mg Na2MoO4·2H2O, 5.5 mg FeSO4·7H2O, 20 mg Na2EDTA·2H2O, 20 mg citric acid, 20 mg thiamine·HCl. In shake flask cultures used 20.9 g 3‐[N‐morpholino]propanesulfonic acid (100 mM MOPS) while for batch processes used 25 mM MOPS. Thiamine was filtered sterilized, and other medium components were autoclaved in compatible mixtures, combined and then adjusted to a pH of 7.1 with 20% (w/v) NaOH.
The 0.8 M sodium acetate buffer (pH 5.8) was made by dissolving 109.2 g sodium acetate trihydrate and 40.0 mL acetic acid (1 M) to 0.8 L ultrapure water, followed by adding ultrapure water to a final volume of 1 L. The 0.2 M sodium acetate buffer (pH 5.0) contained 17.2 g sodium acetate trihydrate and 73.0 mL acetic acid (1 M). An aliquot of 2.9 mL SINICS comprised 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and a 35 μL 0.2 M sodium acetate buffer (pH 5.0). The 0.1 M solutions of L-glutamate acid, GABA, L-alanine, L-serine, and L-lysine were individually prepared using ultrapure water, and diluted to 10 mM as required. The borate buffer was manufactured by adding 4.9 g boric acid into 100.0 mL of water, adjusting the pH to 10.4 with NaOH, and then diluting it to 200.0 mL. The amino acids derivatization reagent was made by dissolving 10 mg o-phthalaldehyde in 2.5 mL acetonitrile, then supplementing 10 μL β-mercaptoethanol. The mobile phase of HPLC consisted of solvent A and solvent B at a volume ratio of 4:1. Solvent A was prepared by mixing 9.8 mL of 20.0 mM sodium acetate buffer (pH 7.3) and 0.2 mL triethylamine. Solvent B was acetonitrile.
The seed medium (pH 5.0) for L. brevis CD0817 consisted of (g/L): yeast extract, 35.0; MnSO4·H2O, 0.05; monosodium L-glutamate, 28.0; tween-80, 1.0; and glucose, 10.0. The fermentation medium was (g/L): yeast extract, 35.0; MnSO4·H2O, 0.05; tween-80, 1.0; glucose, 5.0; monosodium L-glutamate, 33.8; and 650.0 g solid powder L-glutamic acid was added prior to inoculation. Glucose, L-glutamic acid/monosodium L-glutamate, and the other components were individually sterilized at 121 °C for 20 min and mixed together before use.
Top products related to «Sodium Acetate Trihydrate»
More about "Sodium Acetate Trihydrate"
Also known as sodium acetate, this crystalline solid is commonly used as a buffer in biochemical reactions, a food additive, and a de-icing agent.
The compound is composed of sodium (Na), acetate (CH3COO-), and three water molecules (3H2O), forming a unique crystal structure.
Sodium acetate trihydrate is known for its ability to regulate pH levels and maintain a stable environment in various applications.
In research and laboratory settings, sodium acetate trihydrate is often used as a buffer to control the acidity or basicity (pH) of solutions.
This is particularly important in biochemical reactions, such as those involving enzymes, proteins, or other biomolecules, where the pH needs to be carefully maintained for optimal performance.
Aside from its use in biochemistry, sodium acetate trihydrate finds applications in the food industry as a preservative, flavoring agent, and pH regulator.
It is commonly used in the production of pickles, sauces, and other food products to enhance flavor and extend shelf life.
Furthermore, sodium acetate trihydrate has been employed as a de-icing agent, particularly in cold climates, due to its ability to lower the freezing point of water.
This property makes it useful for clearing snow and ice from roads, walkways, and other surfaces.
When working with sodium acetate trihydrate, researchers can benefit from the AI-powered platform provided by PubCompare.ai.
This platform allows seamless access to protocols from literature, preprints, and patents, enabling researchers to identify the optimal protocols and products for their experiments.
By utilizing cutting-edge AI comparisons, researchers can enhance the reproducibility of their work and achieve better results.
In addition to sodium acetate trihydrate, related compounds such as gallic acid, hydrochloric acid, sodium hydroxide, methanol, acetic acid, DPPH, Folin-Ciocalteu reagent, and sodium carbonate may also be relevant in various research and industrial applications.
Exploring the properties and interactions of these substances can provide valuable insights and opportunities for innovation.
Start optimizing your experiments today with PubCompare.ai and unlock the full potential of sodium acetate trihydrate and other related compounds in your research endeavors.