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Sodium Butyrate

Sodium butyrate is a sodium salt of butyric acid, a short-chain fatty acid with important physiological functions.
It is found naturally in the human gut and plays a role in maintaining intestinal health.
Sodium butyrate has been studied for its potential therapeutic applications in conditions like ulcerative colitis, Crohn's disease, and colorectal cancer due to its anti-inflammatory and anti-proliferative properties.
Researchers can use PubCompare.ai's AI-driven platform to discover relevant protocols from literature, pre-prints, and patents, and identify the best protocols and products for their sodium butyrate research.
This can help enhance reproducibility and optimize the research workflow.

Most cited protocols related to «Sodium Butyrate»

1
Comparative Analysis of Perturbed Gene Expression
Cited 50 times
The first dataset consists of nine gene perturbations with matched control samples (Almudevar et al., 2011 ); the second dataset is composed of two cell types and three treatments (Sampson et al., 2013 (link)); the third dataset is a study of the effect of p53 and/or Ras mutations on gene expression (McMurray et al., 2008 (link)).
In the first dataset, cells transformed to malignancy by mutant p53 and activated Ras are perturbed with the aim of restoring gene expression to levels found in non-transformed parental cells via retrovirus-mediated re-expression of corresponding cDNAs or shRNA-dependent stable knock-down. The data contain four to six replicates for each perturbation, and each perturbation has a corresponding control sample in which only the vector has been added (Almudevar et al., 2011 ).
The second dataset consists of two cell types—young adult mouse colon (YAMC) cells and mutant-p53/activated-Ras transformed YAMC cells—in combination with three treatments—untreated, sodium butyrate or valproic acid. Four replicates were performed for each cell-type/treatment combination (Sampson et al., 2013 (link)).
The third dataset is a comparison between four cell types—YAMC cells, mutant-p53 YAMC cells, activated-Ras YAMC cells and p53/Ras double mutant YAMC cells. Three replicates were performed for the untransformed YAMC cells, and four replicates were performed for each of the other cell types (McMurray et al., 2008 (link)).
As in the original publications, all three datasets were normalized to a reference gene, Becn1, with the resulting values denoted as ΔCt. In the first dataset, ΔΔCt values were computed by comparing each perturbed sample to its corresponding control sample. Additional details regarding each of these datasets can be found in the original publications.
McCall M.N., McMurray H.R., Land H, & Almudevar A. (2014). On non-detects in qPCR data. Bioinformatics, 30(16), 2310-2316.
Publication 2014
BECN1 protein, human Cells Cloning Vectors Colon DNA, Complementary Gene Expression Genes Lanugo Malignant Neoplasms Mus Mutation Parent Retroviridae Short Hairpin RNA Sodium Butyrate Valproic Acid Young Adult
2
Nicotinic Receptor Purification and Characterization
Cited 42 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2016
Cells Centrifugation cholesterol-hemisuccinate Crystallization Endoglycosidases Enzymes G-800 Gel Chromatography L Cells Mass Spectrometry Nicotine Proteins SDS-PAGE Sodium Butyrate Sodium Chloride Streptococcal Infections Tissue, Membrane Tromethamine Trypsin Virus
3
HDAC1/2 Overexpression in Tau Mice
Cited 27 times
The mouse HDAC1 or HDAC2 coding sequence was placed into exon 1 of the Tau gene. HDAC2 KO was produced in the laboratory of R.A.D. and engineered to contain loxP recombination sites such that Cre-mediated recombination deletes exons 5 and 6. Sodium butyrate (Sigma) was dissolved in saline. HDAC inhibitors were dissolved in DMSO in 50mg/ml and diluted with saline immediate before injection (100μl–150μl, i.p.). Lysates for immunoblotting were prepared as previously described4 (link). Immunoblot data were quantified by measuring the band intensity using NIH imaging software and UN-SCAN-it gel digitizing software (Silk Scientific). Immunostaining was performed as described previously4 (link) using LSMeta10 software and a confocal microscope (Zeiss). All behavioral testing was performed as described before4 (link). The data were analyzed by unpaired Student’s t-test. Two-way ANOVA was employed to compare difference between groups in several time points. Error bars present s.e.m.
Guan J.S., Haggarty S.J., Giacometti E., Dannenberg J.H., Joseph N., Gao J., Nieland T.J., Zhou Y., Wang X., Mazitschek R., Bradner J.E., DePinho R.A., Jaenisch R, & Tsai L.H. (2009). HDAC2 negatively regulates memory formation and synaptic plasticity. Nature, 459(7243), 55-60.
Publication 2009
Exons Histone Deacetylase Inhibitor Immunoblotting Mice, House Microscopy, Confocal neuro-oncological ventral antigen 2, human Open Reading Frames Radionuclide Imaging Recombination, Genetic Saline Solution Silk Sodium Butyrate Student Sulfoxide, Dimethyl
4
Lentiviral Transduction and Recombination Protocols
Cited 17 times
All cell culture reagents were purchased from ThermoFisher Scientific unless otherwise noted. All cell lines were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 0.1 mg/ml streptomycin (D10). Cells were passaged by detachment with Trypsin-Ethylenediaminetetraacetic acid 0.25%.
Lentiviral vectors were produced by transfection of 250 000 HEK 293T cells in a 6-well with 500 ng pMD-VSV-G (Addgene #12259), 1750 ng PsPax2 (Addgene # 12260) and 1750 ng of the LLP or derivative vector template, using 6 μg of Fugene 6 (Promega). Media was changed the next day, and the supernatant collected over the next 72 h. The collected media was pelleted at 300 × g for 4 min, and the supernatant was passed through a 0.45 μm filter. HEK293T cells, A549 cells, NIH3T3 cells and HT-29 cells were incubated with various dilutions of lentiviral supernatant ranging from 100 μl to 4 ml, and transduced cultures were visually confirmed to have been transduced at MOI clearly less than one. Cells were then incubated with 10 μg/ml of Blasticidin. After a week of selection, cells were assessed with a BD FACS Aria II for fluorescence, and individual BFP+ cells were sorted into separate wells of 96-well plates. Cell clones that grew out were transferred into a 24-well plate and subsequently transferred to 6-well plates for analysis. Cells were induced to express off of the Tet-inducible promoter by adding doxycycline to a final concentration of 2 μg/ml (D10-dox). Cells were also maintained in D10-dox unless otherwise noted.
HEK 293T-based cells were recombined by transfecting 250 000 HEK 293T cells in a 6-well plate, with 1500 ng of pCAG-NLS-Bxb1, and 1500 ng of either individual or a mixture of attB recombination plasmids, incubated with 6 μg of Fugene 6 reagent in doxycycline-free media. Two or more days following transfection, the media was changed to D10-dox media, and cells were assessed for recombination at least 2 days after. For recombination of A549 cells, 150 000 cells plated in a 6-well with 2 ml of media were transfected with a mixture of 3.75 μl Lipofectamine 3000 diluted in 125 μl OPTI-MEM, and 5 μg DNA with 10 μl P3000 reagent diluted in 125 μl OPTI-MEM. For recombination of 3T3 cells, 150 000 cells plated in a 6-well with 2 ml of media were transfected with a mixture of 7.5 μl Lipofectamine 3000 diluted in 125 μl OPTI-MEM and 5 μg DNA with 10 μl P3000 reagent diluted in 125 μl OPTI-MEM. Recombined cells were positively selected by growing the cells for a week in D10-dox media supplemented with the indicated amounts of hygromycin, puromycin or blasticidin (Invivogen). Cells were split and media replenished every 3 days. Recombined cells were negatively selected with the addition of 10 nM AP1903 / Rimiducid (MedChemExpress). Cells were maintained in AP1903 for 2 days. Sodium Butyrate (Sigma) was dissolved as a 0.5 M stock solution in H2O.
Matreyek K.A., Stephany J.J., Chiasson M.A., Hasle N, & Fowler D.M. (2019). An improved platform for functional assessment of large protein libraries in mammalian cells. Nucleic Acids Research, 48(1), e1.
Publication 2019
3T3 Cells A549 Cells AP 1903 reagent Cell Culture Techniques Cell Lines Cells Clone Cells Cloning Vectors Culture Media Doxycycline Edetic Acid Fetal Bovine Serum Fluorescence FuGene HEK293 Cells HT29 Cells hygromycin A Lipofectamine NIH 3T3 Cells NRG1 protein, human Penicillins Plasmids Promega Puromycin Recombination, Genetic Sodium Butyrate Streptomycin Technique, Dilution Transfection Trypsin
5
Generating Recombinant Viral Particles for Transduction
Cited 15 times
FV supernatants containing recombinant viral particles were generated essentially as described previously [51 (link),52 (link)]. Briefly, FV supernatants were produced by cotransfection of 293T cells with transfer vector (puc2MD9 or pMD11), Env- (pczHFVenvEM002), Pol - (pcziPol), and Gag packaging plasmid (pcoPG4 or PG mutants thereof as indicated) at a ratio of 4:4:4:1 using polyethyleneimine (PEI) or Polyfect transfection reagents. At 24 h posttransfection, sodium butyrate (final concentration, 10 mM) was added to the growth medium for 8 h. Subsequently, the medium was replaced, and viral supernatants were harvested an additional 16 h later. Lentiviral supernatants were generated by cotransfection of transfer vector (p6NST90), Gag/Pol packaging plasmid (pCD/NL-BH), and an Env packaging plasmid (pczVSV-G or pczPE01) at a ratio of 1:1:1 and harvested as described above.
Transductions of recombinant EGFP expressing PFV vector particles containing various PFV Gag proteins were performed by infection of 2 × 104 HT1080 cells, plated 24 h in advance in 12-well plates. For the infection 1 ml of the viral supernatant or dilutions thereof were incubated with the target cells for 4 to 6 h. The percentage of EGFP-positive cells was determined by fluorescence-activated cell sorter (FACS) analysis 72 h after infection. All transduction experiments were performed three times, and in each independent experiment the values obtained with the wild-type construct pcoPG4 were arbitrarily set to 100%. Analysis of tissue tropism of different PFV vector particles or HIV-1 vector pseudotypes was performed on various target cell lines. Adherent target cells (HT1080, HeLa, mouseL, Sog9, Pac2), which were plated one day in advance at a density of 2 × 104 cells in 12-well plates, were infected with one ml of viral cell culture supernatant or dilutions thereof. Target cells growing in suspension (Jurkat, G1E-ER4) were infected by resuspending 1 × 105 target cells in one ml of viral cell culture supernatant or dilutions thereof. Afterwards they were transferred into a 6-well plate and either incubated at 37°C, 5% CO2 in a humidified incubator or centrifuged for 1 h at 2000 rpm (30°C) before the incubation step. Sixteen hours later the viral cell culture supernatant was replaced for both types of target cells by fresh media and the transduction efficiency was determined by flow cytometry 72 - 96 h after infection as described above.
Stirnnagel K., Lüftenegger D., Stange A., Swiersy A., Müllers E., Reh J., Stanke N., Große A., Chiantia S., Keller H., Schwille P., Hanenberg H., Zentgraf H, & Lindemann D. (2010). Analysis of Prototype Foamy Virus particle-host cell interaction with autofluorescent retroviral particles. Retrovirology, 7, 45.
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Publication 2010
Cell Culture Techniques Cell Lines Cells Cloning Vectors Flow Cytometry Fluorescence Gene Products, gag HEK293 Cells HeLa Cells HIV-1 Infection Plasmids Polyethyleneimine Sodium Butyrate Technique, Dilution Tissues Transfection Tropism Virion Virus Diseases

Most recents protocols related to «Sodium Butyrate»

1
Baculovirus-Mediated SLCO1B1 Variant Expression
Baculoviruses
carrying either
the reference or variant SLCO1B1 and sodium butyrate
(5 mM final concentration) were added to the HEK293 cells after the
cells were cultured for 24 h in T175 flasks. After 48 h of culturing,
the cells were pelleted by centrifugation (3000g,
15 min) and broken down using a Dounce tissue homogenizer and resuspended
in Tris-sucrose (TS) buffer (10 mM Tris-HEPES, 250 mM sucrose, pH
7.4) while being kept on ice. The cell homogenate was centrifuged
for 30 min (3220 g, 4 °C), separating the larger cellular organelles
and the nucleus in the pellet. The resulting supernatant was subsequently
separated into new tubes and centrifuged (21,000g, 4 °C, 99 min) again, resulting in a pellet containing the
crude cell membrane. The protein sample was suspended in TS buffer,
and the total protein concentration was quantified as previously described.
Häkkinen K., Kiander W., Kidron H., Lähteenvuo M., Urpa L., Lintunen J., Vellonen K.S., Auriola S., Holm M., Lahdensuo K., Kampman O., Isometsä E., Kieseppä T., Lönnqvist J., Suvisaari J., Hietala J., Tiihonen J., Palotie A., Ahola-Olli A.V, & Niemi M. (2023). Functional Characterization of Six SLCO1B1 (OATP1B1) Variants Observed in Finnish Individuals with a Psychotic Disorder. Molecular Pharmaceutics, 20(3), 1500-1508.
Full text: Click here
Publication 2023
Cell Nucleus Cells Centrifugation HEK293 Cells HEPES Lanugo Plasma Membrane Proteins Sodium Butyrate Sucrose Tissues Tromethamine
2
HEK293 Cell Culture and Transduction
HEK293 human kidney
cells were cultured in Dulbecco’s modified Eagle medium (DMEM)
and high-glucose GlutaMax culture medium supplemented with 10% fetal
bovine serum (FBS) at 37 °C, 5% CO2. The cells (0.5
× 106) were seeded in each well of 48-well plates
(Thermo Fisher Scientific Nunc coated with poly-d-lysine
in-house) 24 h prior to transduction with the baculoviruses. To stimulate
the expression of proteins, sodium butyrate was added with the viruses
at a final concentration of 5 mM (as per in-house optimization).
Häkkinen K., Kiander W., Kidron H., Lähteenvuo M., Urpa L., Lintunen J., Vellonen K.S., Auriola S., Holm M., Lahdensuo K., Kampman O., Isometsä E., Kieseppä T., Lönnqvist J., Suvisaari J., Hietala J., Tiihonen J., Palotie A., Ahola-Olli A.V, & Niemi M. (2023). Functional Characterization of Six SLCO1B1 (OATP1B1) Variants Observed in Finnish Individuals with a Psychotic Disorder. Molecular Pharmaceutics, 20(3), 1500-1508.
Full text: Click here
Publication 2023
Baculoviridae Cells Culture Media Eagle Glucose Homo sapiens Poly A Proteins Serum Sodium Butyrate
3
Histone Extraction from AML12 Cells and Liver
AML12 cell pellets (2 × 106 cells) and liver samples were cut into small pieces (60–70 mg) and transferred to an Eppendorf tube. Thereafter, 1 ml of Triton Extraction Buffer (TEB; PBS containing 0.5% Triton X 100 (v/v), 0.02% (w/v) NaN3) + inhibitors (Trichostatin 10 μM, nicotinamide 10 mM, sodium butyrate 50 mM, with protease, and phosphatase inhibitors P0044, P5725, and P8340) were added per 200 mg of tissue/1 × 107 cells, and samples were homogenized. Then, lysates were incubated on rotation for 10 min at 4 °C, and the mixture was centrifuged at 2000 rpm for 10 min at 4 °C. The supernatant was removed, and the pellet was resuspended in 100 μl of 0.2 N HCl and incubated on rotation overnight at 4 °C. Afterwards, samples were centrifuged at 2000 rpm for 10 min at 4 °C, and the supernatant was transferred to a new tube. Next, HCl was neutralized with NaOH 0.2 N, and samples were stored at −80 °C until used.
Sola-García A., Cáliz-Molina M.Á., Espadas I., Petr M., Panadero-Morón C., González-Morán D., Martín-Vázquez M.E., Narbona-Pérez Á.J., López-Noriega L., Martínez-Corrales G., López-Fernández-Sobrino R., Carmona-Marin L.M., Martínez-Force E., Yanes O., Vinaixa M., López-López D., Reyes J.C., Dopazo J., Martín F., Gauthier B.R., Scheibye-Knudsen M., Capilla-González V, & Martín-Montalvo A. (2023). Metabolic reprogramming by Acly inhibition using SB-204990 alters glucoregulation and modulates molecular mechanisms associated with aging. Communications Biology, 6, 250.
Full text: Click here
Publication 2023
Buffers Cells inhibitors Liver Niacinamide Pellets, Drug Peptide Hydrolases Phosphoric Monoester Hydrolases Sodium Azide Sodium Butyrate Tissues trichostatin A Triton X-100
4
Atherosclerosis Progression in Mouse Models
All animal protocols used in this study were approved by the Ethics Committee of Ningxia Medical University (No. 2020–527). Thirty male Jackson (C57BL/6J) mice aged 8 weeks and weighing 18–22 g were purchased from Ningxia Medical Laboratory Animal Center. Thirty male ApoE−/− mice (8-week-old) were obtained from Vital River Laboratory Animal Technology Co., Ltd., Beijing, China. All the mice were maintained under standard, specific, and pathogen-free conditions in individual cages in a temperature-controlled room (ambient temperature 22 ± 1°C, air humidity 40–70%) with a 12 h light/dark cycle in Ningxia Medical Laboratory Animal Center. A high-fat diet (HFD) with 0.5% cholesterol (No. TP28520) was purchased from TROPHIC Animal Feed High-tech Co., Ltd., Nantong, China. The exact product description of HFD and normal diet were supported in S1 Table. Sodium butyrate (NaB, purity>98, No. V900464) was obtained from Sigma (St Louis, MO, USA).
Ma H., Yang L., Liu Y., Yan R., Wang R., Zhang P., Bai Z., Liu Y., Ren Y., Li Y., Jiang X., Wang T., Ma P., Zhang Q., Li A., Guo M., Zhang X., Jia S, & Wang H. (2023). Butyrate suppresses atherosclerotic inflammation by regulating macrophages and polarization via GPR43/HDAC-miRNAs axis in ApoE−/− mice. PLOS ONE, 18(3), e0282685.
Full text: Click here
Publication 2023
Animals Animals, Laboratory Apolipoproteins E Cholesterol Diet, High-Fat Ethics Committees Humidity Males Mice, House Mice, Inbred C57BL pathogenesis Rivers Sodium Butyrate Therapy, Diet
5
Gut Microbiota Alterations in DMD
In this study, we found that in mdx mice, a validated preclinical model of DMD, the disease is associated with a significant alteration in the gut microbiota composition compared with healthy controls. Along with this alteration, the plasma of mdx mice showed a reduction in the levels of gut microbiota‐related metabolites, the short‐chain fatty acids (SCFAs), and an elevation of those of endocannabinoids. Supplementation with the SCFA, sodium butyrate (NaB), rescued muscle strength and autophagy, and prevented inflammation associated with excessive endocannabinoid signaling at CB1 receptors to the same extent as deflazacort (DFZ), the standard palliative care for DMD. In C2C12 myoblasts stimulated with lipopolysaccharide, a pro‐inflammatory molecule derived from a malfunctioning gut microbiota, NaB exerted anti‐inflammatory effects, promoted autophagy, and prevented dysregulation of microRNAs that keep under negative control the CB1 receptor gene and did so in a manner depending on the activation of GPR109A and PPARγ receptors.
Kalkan H., Pagano E., Paris D., Panza E., Cuozzo M., Moriello C., Piscitelli F., Abolghasemi A., Gazzerro E., Silvestri C., Capasso R., Motta A., Russo R., Di Marzo V, & Iannotti F.A. (2023). Targeting gut dysbiosis against inflammation and impaired autophagy in Duchenne muscular dystrophy. EMBO Molecular Medicine, 15(3), e16225.
Full text: Click here
Publication 2023
Anti-Inflammatory Agents Autophagy deflazacort Endocannabinoids Fatty Acids, Volatile Gastrointestinal Microbiome Genes Inflammation Lipopolysaccharides Mice, Inbred mdx MicroRNAs Muscle Strength Myoblasts Palliative Care Plasma PPAR gamma Receptor, Cannabinoid, CB1 Sodium Butyrate

Top products related to «Sodium Butyrate»

1
Sodium butyrate by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, Canada, Macao, Poland
Sodium butyrate is a chemical compound that is commonly used as a laboratory reagent. It is a salt of butyric acid, which is a short-chain fatty acid. Sodium butyrate is a white, crystalline powder that is soluble in water and other polar solvents. Its primary function is to serve as a cell culture supplement in research applications.
2
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
3
Sodium propionate by Merck Group
Sourced in United States, Germany, United Kingdom
Sodium propionate is a chemical compound commonly used as a preservative in the food and pharmaceutical industries. It functions as an antimicrobial agent, inhibiting the growth of mold, yeast, and some bacteria. Sodium propionate is a white, crystalline powder that is soluble in water.
4
Sodium acetate by Merck Group
Sourced in United States, Germany, United Kingdom, Spain, India, Italy, France, China, Poland, Canada, Australia, Ireland, Hungary, Mexico, Chile, Switzerland, Brazil, Macao, Netherlands, Belgium, New Zealand, Portugal, Czechia, Sweden, Sao Tome and Principe
Sodium acetate is a chemical compound with the formula CH3COONa. It is a common salt that is widely used in various laboratory and industrial applications. Sodium acetate functions as a buffer solution, helping to maintain a specific pH level in chemical reactions and processes.
5
Trichostatin a by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, Macao
Trichostatin A is a histone deacetylase (HDAC) inhibitor used in laboratory research. It functions by inhibiting HDAC enzymes, which are involved in the regulation of gene expression. Trichostatin A is commonly utilized in cell-based assays and experiments to study the effects of HDAC inhibition on various biological processes.
6
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
7
Polybrene by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, Macao, Canada, France, Japan, Switzerland, Italy, Australia, Netherlands, Morocco, Israel, Ireland, Belgium, Denmark, Norway
Polybrene is a cationic polymer used as a transfection reagent in cell biology research. It facilitates the introduction of genetic material into cells by enhancing the efficiency of DNA or RNA uptake.
8
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
9
Protease inhibitor cocktail by Roche
Sourced in United States, Switzerland, Germany, China, United Kingdom, France, Canada, Japan, Italy, Australia, Austria, Sweden, Spain, Cameroon, India, Macao, Belgium, Israel
Protease inhibitor cocktail is a laboratory reagent used to inhibit the activity of proteases, which are enzymes that break down proteins. It is commonly used in protein extraction and purification procedures to prevent protein degradation.
10
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

More about "Sodium Butyrate"

Sodium butyrate, a short-chain fatty acid, is a natural compound found in the human gut and plays a crucial role in maintaining intestinal health.
It has been extensively studied for its potential therapeutic applications in conditions like ulcerative colitis, Crohn's disease, and colorectal cancer due to its anti-inflammatory and anti-proliferative properties.
Researchers can utilize PubCompare.ai's AI-driven platform to discover relevant protocols from literature, pre-prints, and patents, and identify the best protocols and products for their sodium butyrate research.
This can help enhance the reproducibility and optimize the research workflow.
Sodium butyrate is closely related to other fatty acids, such as sodium propionate and sodium acetate, which also have important physiological functions.
Additionally, compounds like trichostatin A, a histone deacetylase inhibitor, have been used in conjunction with sodium butyrate to study its epigenetic effects.
To conduct sodium butyrate research, researchers often use cell culture models and employ common laboratory reagents, such as fetal bovine serum (FBS), Lipofectamine 2000 for transfection, Polybrene for viral transduction, and penicillin/streptomycin for antibiotic selection.
Protease inhibitor cocktails may also be used to preserve protein integrity during experiments.
By utilizing PubCompare.ai's AI-driven platform, researchers can streamline their sodium butyrate research, enhance reproducibility, and optimize their workflow, ultimately leading to more efficient and impactful discoveries in the field of intestinal health and disease management.