Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Sodium Citrate

Sodium Citrate

Sodium citrate is a chemical compound that plays a crucial role in various biological processes.
It is a salt of citric acid, commonly used as a pH buffer, anticoagulant, and flavoring agent in a wide range of applications.
Sodium citrate helps maintain the acid-base balance in the body, and it is also found in some food and beverage products.
Researchers use sodium citrate in their studies to investigate its effects on cellular function, blood clotting, and other physiological mechanisms.
Understanding the properties and applications of sodium citrate is important for advancing scientific knowledge and developing new therapies.
With PubCommpare.ai's AI-driven optimization, researchers can effortlessly find the most reproducible and effective sodium citrate protocols from research publications, preprints, and patents, streamlining their experimental process and taking the guesswork out of their work.

Most cited protocols related to «Sodium Citrate»

1
Immunohistochemical Staining of Tumor Tissue
Standard IHC protocol was followed to stain the tumor tissue samples using the mouse monoclonal antibody against hNIS (human Sodium Iodide Symporter) (Abcam, ab17795), ER (Estrogen Receptor) (Abcam, ab16660, ab288). Briefly, 5 µm sized paraffin embedded tissue sections were de-paraffinized with xylene and endogenous peroxidase activity was quenched with 3% H2O2 in methanol for 30 minutes in the dark. Tissue sections were dehydrated through graded alcohols and subjected to antigen retrieval using 10mM sodium citrate. Sections were washed with TBST (Tris Borate Saline Tween-20) and then blocked with 5% BSA (Bovine Serum Albumin) for one hour. Slides were incubated with the respective mouse monoclonal primary antibody diluted with TBS. Slides were then washed for 5 minutes in TBST and incubated for 1 hour with the respective HRP (Horse Raddish Peroxidase) conjugated anti-mouse secondary antibody diluted with TBS in a ratio of 1∶200. After washing, slides were incubated with DAB (3,3′-diaminobenzidine tetrahydrochloride) (Sigma) and immediately washed under tap water after color development. Slides were then counter stained with hematoxylin. Slides were mounted with DPX (dibutyl phthalate xylene) and were then observed under a light microscope (Carl Zeiss).
Varghese F., Bukhari A.B., Malhotra R, & De A. (2014). IHC Profiler: An Open Source Plugin for the Quantitative Evaluation and Automated Scoring of Immunohistochemistry Images of Human Tissue Samples. PLoS ONE, 9(5), e96801.
Full text: Click here
Publication 2014
Antibodies, Anti-Idiotypic Antigens Borates Equus caballus estrogen receptor alpha, human Ethanol Homo sapiens Light Microscopy Methanol Monoclonal Antibodies Mus Neoplasms Paraffin Peroxidase Peroxide, Hydrogen Phthalate, Dibutyl Saline Solution Serum Albumin, Bovine SLC5A5 protein, human Sodium Citrate Stains Tissues Tromethamine Tween 20 Xylene
2
Whole-mount in situ hybridization of planarians
Unless otherwise noted, asexual planarians 1–5 mm in length were processed for WISH essentially as described [21 (link)] with the following significant modifications: the reduction step prior to dehydration was omitted. Bleaching was performed for 2 hours in formamide bleaching solution (1.2% H2O2, 5% formamide, and 0.5xSSC [32 ]). For regenerating planarians, the Proteinase K/post fixation steps were replaced with a 10 minute boiling step in 10 mM sodium citrate pH 6.0 with 0.05% Tween20, followed by a 20 minute room temperature incubation in PBSTx (Phosphate Buffered Saline [32 ], 0.3% Triton X-100) with 1% SDS. Blocking and antibody incubation for peroxidase-conjugated anti-digoxigenin (1:2,000 [Roche]), anti-fluorescein (1:2,000 [Roche]), and anti-dinitrophenol (1:300 [PerkinElmer]) were performed with 5% horse serum and 0.5% RWBR in TNTx (100 mM Tris pH 7.5, 150 mM NaCl, 0.3% Triton X-100). For chromogenic detection using alkaline phosphatase-conjugated anti-digoxigenin antibody (1:2,000 [Roche]), antibody incubation and blocking were performed with 5% horse serum in TNTx, and post-antibody washes were with TNTx prior to development as described in [21 (link)].
King R.S, & Newmark P.A. (2013). In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea. BMC Developmental Biology, 13, 8.
Full text: Click here
Publication 2013
Alkaline Phosphatase Antibodies, Anti-Idiotypic azo rubin S Dehydration Digoxigenin Dinitrophenols Endopeptidase K Equus caballus Fluorescein formamide Immunoglobulins Peroxidase Peroxide, Hydrogen Phosphates Planarians Saline Solution Serum Sodium Chloride Sodium Citrate Triton X-100 Tromethamine Tween 20
3
Immunohistochemical and FISH Analysis of Breast Tumors
The 4046 tumors assembled into tissue microarrays, in BCCA series, were examined with immunohistochemistry and fluorescent in situ hybridization. Immunohistochemistry for ER, PR, HER2, and Ki67 was performed concurrently on serial sections with the standard streptavidin–biotin complex method with 3,3′-diaminobenzidine as the chromogen. Staining for ER, PR, and HER2 interpretation was as described previously (20 (link)). Briefly, the Ki67 antibody (clone SP6; ThermoScientific, Fremont, CA) was applied at a 1:200 dilution for 32 minutes, by following the Ventana Benchmark automated immunostainer (Ventana, Tucson AZ) standard Cell Conditioner 1 (CC1, a proprietary buffer) protocol at 98°C for 30 minutes. ER antibody (clone SP1; ThermoFisher Scientific, Fremont CA) was used at 1:250 dilution with 10-minute incubation, after an 8-minute microwave antigen retrieval in 10 mM sodium citrate (pH 6.0). Ready-to-use PR antibody (clone 1E2; Ventana) was used by following the CC1 protocol as above. HER2 staining was done with the SP3 antibody (ThermoFisher Scientific) at a 1:100 dilution after antigen retrieval in 0.05 M Tris buffer (pH 10.0) with heating to 95°C in a steamer for 30 minutes. For HER2 fluorescent in situ hybridization assay, slides were hybridized with probes to LSI (locus-specific identifier) HER2/neu and to centromere 17 by use of the PathVysion HER-2 DNA Probe kit (Abbott Molecular, Abbott Park, IL) according to manufacturer's instructions, with modifications to pretreatment and hybridization as previously described (29 (link)). Slides were counterstained with 4′,6-diamidino-2-phenylindole, stained material was visualized on a Zeiss Axioplan epifluorescent microscope, and signals were analyzed with a Metafer image acquisition system (Metasystems, Altlussheim, Germany). Biomarker expression from immunohistochemistry assays was scored by two surgical pathologists (T. O. Nielsen and D. Gao), who were blinded to the clinicopathological characteristics and outcome and who used previously established and published criteria for biomarker expression levels that had been developed on other breast cancer cohorts (12 (link),30 (link)). Tumors were considered positive for ER (27 (link)) or PR (31 ) if immunostaining was observed in more than 1% of tumor nuclei, as described previously. Tumors were considered positive for HER2 if immunostaining was scored as 3+ according to HercepTest criteria, with an amplification ratio for fluorescent in situ hybridization of 2.0 or more being the cut point that was used to segregate immunohistochemistry equivocal tumors (scored as 2+) (32 (link)). Ki67 was visually scored for percentage of tumor cell nuclei with positive immunostaining above the background level by two pathologists (T. O. Nielsen and D. Gao). Tissue microarray core samples with fewer than 50 tumor cells were considered uninterpretable (27 (link),28 (link)). All the stained tissue microarrays were digitally scanned, and primary image data are available for public access (http://www.gpecimage.ubc.ca; username, luminalB; password, luminalb).
Cheang M.C., Chia S.K., Voduc D., Gao D., Leung S., Snider J., Watson M., Davies S., Bernard P.S., Parker J.S., Perou C.M., Ellis M.J, & Nielsen T.O. (2009). Ki67 Index, HER2 Status, and Prognosis of Patients With Luminal B Breast Cancer. JNCI Journal of the National Cancer Institute, 101(10), 736-750.
Publication 2009
Acid Hybridizations, Nucleic Antigens azo rubin S Biological Assay Biological Markers biotin-streptavidin complex Breast Carcinoma Buffers Cell Nucleus Cells Centromere Clone Cells DNA Probes ERBB2 protein, human Fluorescent in Situ Hybridization Immunoglobulins Immunohistochemistry Microarray Analysis Microscopy Microwaves Neoplasms Operative Surgical Procedures Pathologists Receptor, ErbB-2 Sodium Citrate Technique, Dilution Tissues Tissue Stains Tromethamine
4
Structural Insights into GluCl Receptor
GluClcryst was expressed from baculovirus-infected Sf9 cells and purified by metal ion affinity chromatography. The Fab complex was isolated by size-exclusion chromatography. The GluClcryst-Fab complex was concentrated to 1-2 mg/mL and supplemented with synthetic lipids and ivermectin. Crystallization was performed by hanging drop vapor diffusion at 4°C with a precipitating solution containing 21-23% PEG 400, 50 mM sodium citrate pH 4.5 and 70 mM sodium chloride. Cryoprotection was achieved by soaking crystals in precipitant solution supplemented with 30% PEG 400. Additional complexes were obtained by soaking crystals in cryoprotectant containing L-glutamate, picrotoxin or sodium iodide. Diffraction data were indexed, integrated and scaled and the structure solved by molecular replacement using a GLIC-derived homology model of GluClcryst and a Fab homology model as search probes. The molecular replacement phases were used to initiate autobuilding and the resulting model was iteratively improved by cycles of manual adjustment and crystallographic refinement. Function of GluCl was examined by two-electrode voltage clamp experiments and by [3H]-L-glutamate saturation and competition binding assays.
Hibbs R.E, & Gouaux E. (2011). Principles of activation and permeation in an anion-selective Cys-loop receptor. Nature, 474(7349), 54-60.
Publication 2011
Baculoviridae Biological Assay Chromatography, Affinity Cryoprotective Agents Crystallization Crystallography Diffusion Gel Chromatography Glutamate Ivermectin Lipids Metals Picrotoxin polyethylene glycol 400 Sf9 Cells Sodium Chloride Sodium Citrate Sodium Iodide
5
RNA Extraction from Microbial Cells
The extractions using the "BPC" method were performed as previously described [24 (link)], except that no 0.1 mm beads were used and the bead beating settings were not the same. All procedures were carried out at room temperature and centrifugations at 10000 g. The concentrated cell samples were centrifuged for 3 minutes and resuspended in 800 μl of water and 600 μl of buffer saturated phenol (pH 4.3). Addition of 0.5 g of 0.5 mm glass beads was followed by homogenization using a Bertin Precellys 24 (maximum speed, 20 seconds, twice). The sample tubes were then centrifuged for 10 minutes after which 750 μl of aqueous layer was transferred to a fresh tube, mixed with same volume of buffer saturated phenol (pH 4.3) and vortexed for 30 seconds. This was followed by centrifugation for 5 minutes and removal of 700 μl of aqueous supernatant that was then mixed with same volume of chloroform, in fresh tubes. After vortexing for 30 seconds, the samples were centrifuged for 5 minutes. At this point, 650 μl of aqueous layer were transferred to a fresh tube to which 65 μl of 3 M sodium acetate and 650 μl of isopropanol were added. The tubes were vortexed for 30 seconds and stored at -20°C for 10 minutes. After centrifugation for 5 minutes, the supernatant was removed and the pellet washed with 1 mL of 70% ethanol. One other centrifugation followed (5 minutes) and after removing the supernatant and air drying the RNA 50 μl of RNA storage solution (1 mM sodium citrate, pH 6.4, Ambion, Austin, USA) was added. The samples were stored at -80°C until analyzed.
Pinto F.L., Thapper A., Sontheim W, & Lindblad P. (2009). Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria. BMC Molecular Biology, 10, 79.
Full text: Click here
Publication 2009
austin Buffers Cells Centrifugation Chloroform Ethanol Isopropyl Alcohol Neoplasm Metastasis Phenol Sodium Acetate Sodium Citrate

Most recents protocols related to «Sodium Citrate»

1
Cellulase Compositions for Sugar Snake Degradation
Not available on PMC !

Example 3

Multiple enzyme compositions comprising a cellulase were evaluated to determine sugar snake degradation performance as a baseline without potential surfactant synergy to assess the role of the enzyme composition versus improvement based on surfactant synergy. Compositions were prepared with 0.5 wt. % enzyme composition, 1.7 wt. % sodium citrate buffer, and water at a pH of 4.25. The four enzyme compositions tested were obtained from Novozymes and included: DRAIN EASE FLOW™, CELLUCLEAN CLASSIC 700T®, CELLUCLAST CONCENTRATED BG®, and Cellulase C. A sugar snake of equal mass was measured and the cleaning compositions were applied to it. The percent degradation of the sugar snake (based on mass) was evaluated after 2 hours of contact and after 24 hours of contact. The percent degradation is shown in FIG. 2 where 100 indicates 100% degradation. As can be seen in FIG. 2, CELLUCLAST CONCENTRATED BG® provided the best sugar snake degradation at both the 2-hour time and 24-hour time. DRAIN EASE FLOW™ and Cellulase C performed substantially similar and CELLUCLEAN CLASSIC 700T® did not appear to degrade the sugar snake at all.

US11859157B2. Synergistic cellulase-surfactant interactions for degradation of bacterial cellulose (2024-01-02). ECOLAB USA INC. [US]. Inventors: Jesse Ray Murphy [US], Lyndal Jensen [US].
Full text: Click here
Patent 2024
Bacteria Buffers Carbohydrates Cellulase Cellulose Endoglucanase C Enzymes Snakes Sodium Citrate Surface-Active Agents Vision
2
Liquisoft Capsule Composition and Evaluation
Not available on PMC !

Example 8

Exemplary capsule shell and matrix compositions useful for producing Liquisoft capsules as described herein are shown in Table 10. Composition components are set forth by weight percentage of the total weight of the composition. Such compositions may be encapsulated using rotary die encapsulation as described herein.

Formulas 14, 15, and 16 were the initial matrix prototypes for dextromethorphan hydrobromide (30 mg) and menthol (5 mg). Three different taste-masking agents were tested: mannitol, thaumatin (Talin®) and glycyrrhizic acid salts (MagnaSweet®). Thaumatin resulted in the most effective taste masking of the dextromethorphan hydrobromide, but resulted in a hazy appearance.

TABLE 10
Exemplary Liquisoft Composition
Matrix Formulation
ComponentFormula 14Formula 15Formula 16
Propylene Glycol8.18.18.1
Polyethylene Glycol 40025.4 25.4 25.4 
Polyvinylpyrrolidone K301.51.51.5
Maltitol50.0 50.0 50.0 
Sucralose0.60.60.6
Citric Acid1.01.01.0
Lactic Acid1.01.01.0
Sodium Citrate1.01.01.0
Mannitol3.0——
Thaumatin (Talin ®)—3.0—
Glycyrrhizic acid salts——3.0
(MagnaSweet ®)
Water5.05.05.0
Dextromethorphan3.03.03.0
Hydrobromide
Menthol0.50.50.5
TOTAL100%100%100%

US11872307B2. Liquisoft capsules (2024-01-16). PATHEON SOFTGELS INC. [US]. Inventors: YinYan Zhao [US], Yunhua Hu [US], Mervin Williams, Jr. [US], Tatyana Dyakonov [US], Saujanya Gosangari [US], Chue Yang [US], Henricus Marinus Gerardus Maria Van Duijnhoven [NL], Martin Piest [NL], Aqeel A. Fatmi [US].
Full text: Click here
Patent 2024
Capsule Citric Acid Dextromethorphan Hydrobromide Glycyrrhizic Acid Lactic Acid maltitol Mannitol Menthol polyethylene glycol 400 Povidone Propylene Glycol Salts Sodium Citrate sucralose Talin Taste
3
Enzyme Stability in Cleaning Compositions
Not available on PMC !

Example 5

Enzyme stability was tested in cleaning compositions prepared with differing stabilizers. All test compositions were prepared containing 0.5% DRAIN EASE FLOW™ 2% Tween® 20, and 1.8% sodium citrate buffer in water prepared at a pH of about 4.5 Enzyme stability was assessed by an activity assay. The results are provided in FIG. 4. Formulations containing 20% PEG 400® and 20% glycerol showed 84% and 83% retention of DRAIN EASE FLOW™ activity after 8 weeks at 37° C. Formulations containing 20% propylene glycol showed 100% retention of DRAIN EASE FLOW™ activity under the same conditions. Indicating the stabilizers did provide enzyme stability and retention.

US11859157B2. Synergistic cellulase-surfactant interactions for degradation of bacterial cellulose (2024-01-02). ECOLAB USA INC. [US]. Inventors: Jesse Ray Murphy [US], Lyndal Jensen [US].
Full text: Click here
Patent 2024
Bacteria Biological Assay Buffers Cellulase Cellulose Enzyme Stability Glycerin polyethylene glycol 400 Propylene Glycol Retention (Psychology) Sodium Citrate Surface-Active Agents Tween 20
4
Formulation and Sensory Evaluation of Low-Calorie Carbonated Beverages
Not available on PMC !

EXAMPLE 7

Low-Calorie Carbonated Beverage

A carbonated beverage according to formula presented below was prepared.

IngredientsQuantity, %
Sucrose5.5
Cola flavor0.340
ortho-Phosphoric acid0.100
Sodium citrate0.310
Sodium benzoate0.018
Citric acid0.018
Rebaudioside A0.003
Glucosyl stevia composition0.05
Carbonated waterto 100

The sensory properties were evaluated by 20 panelists. The results are summarized in Table 4.

TABLE 4
Evaluation of low-calorie carbonated beverage samples
Number of panelists detected the attribute
Taste Sample ,Sample Sample Sample
attributeNo. 1aNo. 2aNo. 3No. 4
Bitter taste00220
Astringent10320
taste
Aftertaste10220
Comments
Quality ofClean Clean Clean Bitter aftertaste
sweet taste(19 of 20)(20 of 20)(17 of 20)(5 of 20)
OverallSatisfactory Satisfactory Satisfactory Satisfactory
evaluation(18 of 20)(20 of 20)(15 of 20)(3 of 20)

The above results show that the beverages prepared using Samples 1a and 2a possessed the best organoleptic characteristics.

US11871771B2. Glucosyl Stevia composition (2024-01-16). PureCircle Sdn Bhd [MY]. Inventors: Avetik Markosyan [AM].
Full text: Click here
Patent 2024
Astringents Benzoate Beverages Carbonated Beverages Carbonated Water Citrate Citric Acid Cola Flavor Enhancers Phosphoric Acids Sodium Benzoate Sodium Citrate Stevia Sucrose Taste
5
Formulation and Sensory Evaluation of Low-Calorie Carbonated Beverage
Not available on PMC !

EXAMPLE 15

Low-Calorie Carbonated Beverage

A carbonated beverage according to formula presented below was prepared.

IngredientsQuantity, %
Sucrose5.5
Cola flavor0.340
ortho-Phosphoric acid0.100
Sodium citrate0.310
Sodium benzoate0.018
Citric acid0.018
Rebaudioside A0.003
Glucosyl stevia composition0.05
Carbonated waterto 100

The sensory properties were evaluated by 20 panelists. The results are summarized in Table 6.

TABLE 6
Evaluation of low-calorie carbonated beverage samples
Number of panelists detected the attribute
TasteSample Sample Sample Sample
attributeNo. 1bNo. 2bNo. 3No. 5
Bitter taste001012
Astringent101515
taste
Aftertaste101318
Comments
Quality ofCleanCleanCleanBitter aftertaste
sweet taste(18 of 20)(20 of 20)(14 of 20)(10 of 20)
OverallSatisfactorySatisfactorySatisfactorySatisfactory
evaluation(19 of 20)(20 of 20)(11 of 20)(9 of 20)

The above results show that the beverages prepared using Samples 1b and 2b possessed the best organoleptic characteristics.

US11871771B2. Glucosyl Stevia composition (2024-01-16). PureCircle Sdn Bhd [MY]. Inventors: Avetik Markosyan [AM].
Full text: Click here
Patent 2024
Astringents Benzoate Beverages Carbonated Beverages Carbonated Water Citrate Citric Acid Cola Flavor Enhancers Phosphoric Acids Sodium Benzoate Sodium Citrate Stevia Sucrose Taste

Top products related to «Sodium Citrate»

1
Sodium citrate by Merck Group
Sourced in United States, Germany, United Kingdom, China, India, Spain, Italy, Switzerland, France, Brazil, Canada, Australia, Ireland, Sao Tome and Principe, Mexico, Macao, Portugal, Austria
Sodium citrate is a chemical compound commonly used in laboratory settings. It is a salt of citric acid and serves as a buffering agent, helping to maintain a specific pH level in solutions. Sodium citrate is a white, crystalline powder that is soluble in water.
2
Streptozotocin (stz) by Merck Group
Sourced in United States, Germany, China, Sao Tome and Principe, United Kingdom, India, Japan, Macao, Canada, France, Italy, Switzerland, Egypt, Poland, Hungary, Denmark, Indonesia, Singapore, Sweden, Belgium, Malaysia, Israel, Spain, Czechia
STZ is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and experiments. The core function of STZ is to serve as a tool for carrying out specific tasks or procedures in a laboratory setting. No further details or interpretation of its intended use are provided.
3
In situ cell death detection kit by Roche
Sourced in Germany, United States, Switzerland, China, United Kingdom, France, Canada, Belgium, Japan, Italy, Spain, Hungary, Australia
The In Situ Cell Death Detection Kit is a laboratory product designed for the detection of programmed cell death, or apoptosis, in cell samples. The kit utilizes a terminal deoxynucleotidyl transferase (TdT) to label DNA strand breaks, allowing for the visualization and quantification of cell death. The core function of this product is to provide researchers with a tool to study and analyze cell death processes.
4
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, France, Canada, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
5
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
6
4 6 diamidino 2 phenylindole (dapi) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, Canada, Italy, Australia, France, Switzerland, Spain, Belgium, Denmark, Panama, Poland, Singapore, Austria, Morocco, Netherlands, Sweden, Argentina, India, Finland, Pakistan, Cameroon, New Zealand
DAPI is a fluorescent dye used in microscopy and flow cytometry to stain cell nuclei. It binds strongly to the minor groove of double-stranded DNA, emitting blue fluorescence when excited by ultraviolet light.
7
Facscalibur by BD
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
8
4 6 diamidino 2 phenylindole (dapi) by Merck Group
Sourced in United States, Germany, Japan, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Canada, Switzerland, Spain, Australia, Denmark, India, Poland, Israel, Belgium, Sweden, Ireland, Netherlands, Panama, Brazil, Portugal, Czechia, Puerto Rico, Austria, Hong Kong, Singapore
DAPI is a fluorescent dye that binds strongly to adenine-thymine (A-T) rich regions in DNA. It is commonly used as a nuclear counterstain in fluorescence microscopy to visualize and locate cell nuclei.
9
Propidium iodide by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Macao, Canada, Spain, India, Belgium, Australia, Israel, Switzerland, Poland, Ireland, Argentina, Austria, Brazil, Sweden, Portugal, New Zealand, Netherlands, Slovakia, Norway, Hungary, Czechia, Denmark
Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.
10
Vacutainer by BD
Sourced in United States, United Kingdom, Brazil, Germany, Canada, France, Spain, Italy, Switzerland, Australia, Sweden, Belgium, New Zealand, Denmark, Mexico, Jersey, South Sudan, Austria, Japan
The BD Vacutainer is a blood collection system used to collect, process, and preserve blood samples. It consists of a sterile evacuated glass or plastic tube with a closure that maintains the vacuum. The Vacutainer provides a standardized method for drawing blood samples for laboratory analysis.

More about "Sodium Citrate"

Sodium Citrate is a versatile chemical compound that plays a crucial role in various biological processes.
Also known as sodium 2-hydroxypropane-1,2,3-tricarboxylate, this salt of citric acid is widely used as a pH buffer, anticoagulant, and flavoring agent across a range of applications.
Researchers often utilize Sodium Citrate in their studies to investigate its effects on cellular function, blood clotting, and other physiological mechanisms.
Understanding the properties and applications of this compound is essential for advancing scientific knowledge and developing new therapies.
In addition to its use as a buffer and anticoagulant, Sodium Citrate has several other important applications.
It can be found in some food and beverage products, where it helps maintain the acid-base balance.
Researchers may also use Sodium Citrate in conjunction with other compounds, such as STZ (Streptozocin), to induce diabetes in animal models for the study of related conditions.
The In Situ Cell Death Detection Kit, which employs Sodium Citrate, is a valuable tool for researchers investigating cellular processes and apoptosis.
Triton X-100 and Bovine Serum Albumin are also commonly used in experiments involving Sodium Citrate, as they can help with cell permeabilization and blocking, respectively.
Flow cytometry techniques, such as those using the FACSCalibur instrument, often require the use of Sodium Citrate and other reagents like Propidium Iodide to analyze cellular characteristics and DNA content.
By utilizing PubCompare.ai's AI-driven optimization, researchers can effortlessly find the most reproducible and effective Sodium Citrate protocols from a vast array of research publications, preprints, and patents.
This streamlines the experimental process and takes the guesswork out of their work, ultimately accelerating scientific discoveries and advancements.