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Sphingolipids

Sphingolipids are a class of lipids that play crucial roles in cellular signaling, membrane structure, and cellular function.
They are composed of a sphingoid base, a long-chain amino alcohol, and various headgroup moieties.
Sphingolipids are involved in a wide range of biological processes, including cell growth, differentiation, and apoptosis.
Dysregulation of sphingolipid metabolism has been implicated in a number of diseases, such as neurodegenerative disorders, cancer, and metabolic diseases.
Resaerch into the mechanismms and functions of sphingolipids is an active area of investigation, with numerous published methods, pre-prents, and patents describing analytical techniques and experimental approaches.
PubCompare.ai's AI-driven platform can help optimize your Sphingolipid resaerch by enabling you to discover, compare, and identify the best published methods to enhance reproducibility and accelerate your work.

Most cited protocols related to «Sphingolipids»

1
Targeted Metabolite Profiling in Urine via DFI-MS/MS
Cited 32 times
To assess the performance of direct flow injection DFI-MS/MS methods in urine metabolomics and to determine the concentration ranges of a number of metabolites not measurable by other methods, we used the commercially available Absolute-IDQ p180 Kit (BIOCRATES Life Sciences AG - Austria). The kit, in combination with an ABI 4000 Q-Trap (Applied Biosystems/MDS Sciex) mass spectrometer, can be used for the targeted identification and quantification of 187 different metabolites or metabolite species including amino acids, biogenic amines, creatinine, acylcarnitines, glycerophospholipids, sphingolipids and hexoses. This method involves derivatization and extraction of analytes from the biofluid of interest, along with selective mass spectrometric detection and quantification via multiple reactions monitoring (MRM). Isotope-labeled internal standards are integrated into the kit plate filter to facilitate metabolite quantification. Metabolite concentrations were expressed as ratios relative to creatinine to correct for dilution, assuming a constant rate creatinine excretion for each urine sample (see Method S3 for additional information).
Bouatra S., Aziat F., Mandal R., Guo A.C., Wilson M.R., Knox C., Bjorndahl T.C., Krishnamurthy R., Saleem F., Liu P., Dame Z.T., Poelzer J., Huynh J., Yallou F.S., Psychogios N., Dong E., Bogumil R., Roehring C, & Wishart D.S. (2013). The Human Urine Metabolome. PLoS ONE, 8(9), e73076.
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Publication 2013
acylcarnitine Amino Acids Biogenic Amines Creatinine Glycerophospholipids Hexoses Isotopes Mass Spectrometry Sphingolipids Tandem Mass Spectrometry Technique, Dilution Urine
2
Comprehensive Serum Metabolite Profiling via UPLC-MS
Cited 27 times
A UPLC-single quadrupole-MS amino acid analysis system was combined with two separate UPLC-time-of-flight (TOF)-MS based platforms analyzing methanol and chloroform/methanol serum extracts. Identified ion features in the methanol extract platform included NEFA, acyl carnitines, bile acids, monoacylglycerophospholipids, monoetherglycerophospholipids, free sphingoid bases, and oxidized fatty acids. The chloroform/methanol extract platform provided coverage over glycerolipids, cholesterol esters, sphingolipids, diacylglycerophospholipids, and acyl-ether-glycerophospholipids. The metabolite extraction procedure was as follows for each platform (lipid nomenclature follows the LIPID MAPS convention – www.lipidmaps.org):
Chromatographic separation and mass spectrometric detection conditions employed for each platform are summarized in Supplementary Table 1. Representative base peak ion chromatograms corresponding to the UPLC-TOF platforms are shown in Figure 1. Online tandem mass spectrometry (MS/MS) experiments for metabolite identification were performed on a Waters QTOF Premier (Waters Corp.) and a Waters SYNAPT G2 instrument, operating in both the positive and negative ion electrospray modes, as described in detail previously14 (link).
Barr J., Caballería J., Martínez-Arranz I., Domínguez-Díez A., Alonso C., Muntané J., Pérez-Cormenzana M., García-Monzón C., Mayo R., Martín-Duce A., Romero-Gómez M., Iacono O.L., Tordjman J., Andrade R.J., Pérez-Carreras M., Le Marchand-Brustel Y., Tran A., Fernández-Escalante C., Arévalo E., García–Unzueta M., Clement K., Crespo J., Gual P., Gómez-Fleitas M., Martínez-Chantar M.L., Castro A., Lu S.C., Vázquez-Chantada M, & Mato J.M. (2012). Obesity dependent metabolic signatures associated with nonalcoholic fatty liver disease progression. Journal of Proteome Research, 11(4), 2521-2532.
Publication 2012
acylcarnitine Amino Acids Bile Acids Chloroform Cholesterol Esters Chromatography Conferences Ethyl Ether Fatty Acids Glycerophospholipids Lipids Mass Spectrometry Methanol Microtubule-Associated Proteins Nonesterified Fatty Acids Serum Sphingolipids Tandem Mass Spectrometry
3
Simultaneous Sphingolipid and Ceramide Quantification
Cited 18 times
The concentration of the 10 sphingolipids and isotopic enrichment of [13C16]16:0-ceramide in muscle were simultaneously measured against an extracted concentration standard curve as well as an enrichment standard curve on a Thermo TSQ Quantum Ultra mass spectrometer (Waltham, MA) coupled with a Waters Acquity UPLC system (Milford, MA). The sphingolipids were separated on the UPLC with a Waters Acquity C8 UPLC BEH column 2.1 × 150 mm, 1.7 μm at 43°C using two buffers. Buffer A was methanol, 2 mM ammonium formate, 0.1% formic acid; buffer B was water, 1 mM ammonium formate, 0.1% formic acid. The flow rate was 0.4 ml/min, and the gradient conditions were as follows: 0 min at 20% B, 0–1.5 min 20-10% B, 1.5–2.3 min isocratic at 10% B, 2.3–9.3 min 10-1%B, 9.3–11 min isocratic at 1%B, 11–11.3 min 1–20%B, 11.3–13 min isocratic at 20%B. Standards and samples were re-suspended in 50 μl buffer A prior to injecting 5 μl onto the UPLC/MS/MS. Figure 1 shows the separation of all species in the standards (panel A) and muscle (panel B).
The mass spectrometer was equipped with an electrospray ionization interface. The following conditions were used: the spray voltage set at 4000V, sheath gas at 0.675 L/min, ion sweep gas at 0.6 L/min, aux gas at 1.2 L/min, and transfer capillary at 275°C. The collision gas was set at 1.2 mTorr. All sphingolipids, except C16:0-Cer, were monitored as [M+H]+ in positive mode. The C16:0-Cer and [13C16]16:0-Cer were monitored as [M+2+H]+ and [M+16+H]+ respectively. Transition of masses and collision energy are shown in Table 1. The entire analysis was performed in SRM mode.
Blachnio-Zabielska A.U., Persson X.M., Koutsari C., Zabielski P, & Jensen M.D. (2012). A liquid chromatography/tandem mass spectrometry method for measuring the in vivo incorporation of plasma free fatty acids into intramyocellular ceramides in humans. Rapid Communications in Mass Spectrometry, 26(9), 1134-1140.
Publication 2012
Buffers Capillaries Ceramides formic acid formic acid, ammonium salt GAS6 protein, human Isotopes Methanol Muscle Tissue Sphingolipids Tandem Mass Spectrometry
4
Lipid Extraction from Barley Roots
Cited 13 times
For lipid extraction, frozen roots were homogenized into a fine powder using a mortar and pestle and liquid nitrogen. Lipids were extracted according to the procedure previously described for sphingolipid extraction in plant tissues (Markham et al., 2006 (link)). Homogenized frozen barley root powder (250−300 mg, exact weight of each sample was recorded) was quickly resuspended with a monophasic mixture of 2-propanol/hexane/water 60:26:14 (v/v/v, 6 ml) and incubated at 60°C for 30 min in an Eppendorf Thermomixer Comfort (Hamburg, Germany) mixing the solutions at 500 rpm. Samples were vortexed for 10 s and sonicated for 1 min every 10 min during incubation. The extracts were centrifuged at 2,000 g for 20 min at room temperature. The supernatant was transferred to a new tube, evaporated to dryness under a stream of nitrogen, then reconstituted in 500 μl of 2-propanol/methanol/water 4:4:1 (v/v/v) and stored at −20°C. A total of five biological replicates with each replicate combining two random plants were prepared. In order to compensate for variations in sample preparation and ionization efficiency, a total of 10 μl of an internal standard mixture, consisting of 100 μM of PE(12:0/12:0) and Cer(d18:1/12:0), was spiked into each replicate prior to extraction. A pooled biological quality control (PBQC) sample was produced by collecting 150 μl from each replicate as described previously (Hill et al., 2014 (link)). PBQC samples were used to monitor the reproducibility within and between different sample batches.
Yu D., Boughton B.A., Hill C.B., Feussner I., Roessner U, & Rupasinghe T.W. (2020). Insights Into Oxidized Lipid Modification in Barley Roots as an Adaptation Mechanism to Salinity Stress. Frontiers in Plant Science, 11, 1.
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Publication 2020
1-Propanol Biopharmaceuticals DNA Replication Freezing Hexanes Hordeum vulgare Lipids Methanol Nitrogen Plant Roots Plants Powder Sphingolipids Tissues
5
Comprehensive Lipid Profiling by Mass Spectrometry
Cited 12 times
Lipid extraction and measurement of lipid metabolites by LC-MS(/MS) was performed as reported previously [21] (link). In brief, small aliquots of frozen plasma and serum were thawed on ice for 2 h. Our normal samples were thus frozen and thawed twice in total, including the dispensing process described above. Lipid metabolites were extracted from 100 µl of plasma or serum by using the method described by Bligh and Dyer (BD) [25] (link) with a few modifications [21] (link). Lower organic layers were measured by ultra-performance liquid chromatography-time of flight mass spectrometry (UPLC-TOFMS; LCT Premier XE; Waters Micro-mass, Waters, Milford, MA) for analysis of phosphoglycerolipids (PLs), sphingolipids (SLs), and neutral lipids (NLs). To distinguish alkenylacyl and alkyl PL species with the same exact mass, a small aliquot of each BD sample was acid-hydrolyzed [26] (link) and analyzed by UPLC-TOFMS. Upper aqueous layers were subjected to solid extraction to obtain polyunsaturated fatty acids (PUFAs) and their oxidative fatty acids (oxFAs), and then measured by UPLC-MS/MS using a 5500QTRAP quadrupole-linear ion trap hybrid mass spectrometer (AB Sciex, Framingham, MA) interfaced with an ACQUITY UPLC System (Waters, Milford, MA). Structural analysis of PLs and SLs was performed by LC-Fourier Transform Mass Spectrometry (LC-FTMS; LTQ Orbitrap XL, Thermo Fisher Scientific, Waltham, MA) as previously described [26] (link), with a few modifications. Data-dependent MS3 analysis was performed in the positive-ion mode to identify the long chain base of ceramides and cerebrosides.
Ishikawa M., Maekawa K., Saito K., Senoo Y., Urata M., Murayama M., Tajima Y., Kumagai Y, & Saito Y. (2014). Plasma and Serum Lipidomics of Healthy White Adults Shows Characteristic Profiles by Subjects’ Gender and Age. PLoS ONE, 9(3), e91806.
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Publication 2014
Acids Ceramides Cerebrosides Fatty Acids Freezing Hybrids Lipids Liquid Chromatography Mass Spectrometry Plasma Polyunsaturated Fatty Acids Serum Sphingolipids Tandem Mass Spectrometry

Most recents protocols related to «Sphingolipids»

1
Comprehensive Metabolomic Profiling via LC-MS/MS
The metabolomic profile was assessed with a validated targeted metabolomics approach, implementing liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and using the AbsoluteIDQ p180 kit (Biocrates Life Sciences AG AbsoluteIDQ® p180 Kit, Innsbruck, Austria), which benefits of an established good interlaboratory reproducibility (27 (link)). Briefly, the serum samples were placed on a 96-well plate pre-loaded with the isotopic labeled internal standards, along with a phosphate buffer solution as blank sample, a calibration curve (7 levels), and three levels of quality control samples. Two different plates were implemented for this study. The sample preparation consisted in the derivatization of amino acids and biogenic amines with phenyl isothiocyanate, evaporation, extraction with 5 mM ammonium acetate in methanol, centrifugation, and dilution. Amino acids and biogenic amines were separated and analyzed through an analytical column before the mass spectrometry (LC-MS/MS), while lipids and the hexose were analyzed with a simple flow injection analysis (FIA-MS/MS). A total of 188 metabolites were measured, including 21 amino acids, 21 biogenic amines, the sum of hexoses, 40 acylcarnitine, 15 sphingolipids (SM), and 90 glycerophospholipids among which 14 lysophosphatidylcholines (LysoPC), 38 diacylphosphatidylcholine (PC aa), and 38 acylalkylphosphatidylcholine (PC ae). Further instrumental and analytical details have been previously reported (28 (link)).
Borroni E., Frigerio G., Polledri E., Mercadante R., Maggioni C., Fedrizzi L., Pesatori A.C., Fustinoni S, & Carugno M. (2023). Metabolomic profiles in night shift workers: A cross-sectional study on hospital female nurses. Frontiers in Public Health, 11, 1082074.
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Publication 2023
acylcarnitine Amino Acids ammonium acetate Biogenic Amines Buffers Centrifugation Flow Injection Analysis Glycerophospholipids Hexoses Isotopes Lipids Liquid Chromatography Lysophosphatidylcholines Mass Spectrometry Methanol phenylisothiocyanate Phosphates Serum Sphingolipids Tandem Mass Spectrometry Technique, Dilution
2
Quantification of Sphingolipid Metabolites in Biological Samples
Sphingolipid metabolites in blood, plasma, and lung were quantified by liquid chromatography electrospray ionization–tandem mass spectrometry (LC‐ESI‐MS/MS) as previously described.36 Briefly, 50 μL of whole blood was used for the determination of sphingolipids. To obtain plasma, fresh whole blood was centrifuged (2000× g, 15 min, 4°C), and the supernatant was removed and stored at −80°C for later analysis.37 Weighed lung tissues were placed into 13 × 100 mm borosilicate tubes with a Teflon‐lined cap and 2 mL of CH3OH and 1 mL of CHCl3. Internal standards (Avanti Polar Lipids, Alabaster, AL) in 10 μL ethanol:methanol:water (7:2:1) as a cocktail of 250 pmol each were added to samples. Standards for sphingoid bases and sphingoid base 1‐phosphates were all 17‐carbon chain length analogs. Samples were dispersed by sonication at room temperature for 30 s. The single‐phase mixtures were incubated at 48°C for 8 h. The extracts were centrifuged and the supernatants transferred to new tubes. The extracts were dried with a speed vac and reconstituted in 0.5 mL of the starting mobile phase solvent for LC‐ESI‐MS/MS analysis, briefly sonicated and centrifuged, and supernatants transferred to autoinjector vials. Sphingolipids were separated by reverse‐phase HPLC using a Supelco 2.1 × 50 mm Ascentis Express C18 column (Sigma) with a binary solvent system at a flow rate of 0.5 mL/min with a column oven set at 35°C. Prior to injection of samples, the column was equilibrated for 0.5 min with a solvent mixture of 95% mobile phase A1 (CH3OH/H2O/HCOOH, 58/41/1, v/v/v, with 5 mM ammonium formate) and 5% mobile phase B1 (CH3OH/HCOOH, 99/1, v/v, with 5 mM ammonium formate), and after sample injection, the A1/B1 ratio was maintained at 95/5 for 2.25 min, followed by a linear gradient to 100% B1 over 1.5 min, which was held at 100% B1 for 5.5 min, followed by a 0.5 min gradient return to 95/5 A1/B1. The column was re‐equilibrated with 95:5 A1/B1 for 0.5 min before each run. The HPLC column was coupled to a Sciex 5500 quadrupole/linear ion trap (QTrap; SCIEX Framingham, MA) operating in triple quadrupole mode. Q1 and Q3 were set to pass molecularly distinctive precursor and product ions (or a scan across multiple m/z in Q1 or Q3), using N2 to collisionally induce dissociations in Q2 (which was offset from Q1 by 30–120 eV). The temperature of the ion source was set at 300°C.
James B.N., Weigel C., Green C.D., Brown R.D., Palladino E.N., Tharakan A., Milstien S., Proia R.L., Martin R.K, & Spiegel S. (2023). Neutrophilia in severe asthma is reduced in Ormdl3 overexpressing mice. The FASEB Journal, 37(3), e22799.
Publication 2023
Alabaster ARID1A protein, human BLOOD Carbon Chloroform Ethanol formic acid, ammonium salt High-Performance Liquid Chromatographies Lipids Liquid Chromatography Lung Methanol Phosphates Plasma Radionuclide Imaging Solvents Spectrometry, Mass, Electrospray Ionization Sphingolipids Teflon Tissues
3
Metabolomic Profiling Stability Analysis

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Publication 2023
Chromatography Diglycerides Endocannabinoids ethanolamine oleoyl ethanolamine Fatty Acids Glycerin Isomerism Oxylipins prisma Sphingolipids Triglycerides Tryptophan vaccenic acid
4
Comprehensive Analytical Reagents for Metabolomic Studies

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Publication 2023
Acetic Acid Acetone acetonitrile Alabaster Anabolism Butanols Caimans Chloroform Citric Acid Endocannabinoids ethyl acetate formic acid Hexanes Hydrochloric acid Isopropyl Alcohol Isotopes Lipids Methanol Oxylipins sodium phosphate, dibasic Sphingolipids Tryptophan
5
Identifying Lipid Metabolism Regulatory Genes in Glioma
Cited 1 time
The LMRGs were obtained from the Molecular Signature Database (MsigDB, v7.5.1, https://www.gsea-msigdb.org/) (22 (link)), including the following pathways: glycerophospholipid metabolism, adipocytokine signing pathway, PPAR signaling pathway, glycerolipid metabolism, regulation of lipolysis in adipocytes, fatty acid metabolism, arachidonic acid metabolism, sphingolipid metabolism, cholesterol metabolism, fatty acid degradation, ether lipid metabolism, steroid hormone biosynthesis, fatty acid elongation, fat digestion and absorption, biosynthesis of unsaturated fatty acids, steroid biosynthesis, linoleic acid metabolism, alpha-linolenic acid metabolism, primary bile acid biosynthesis (Table S1). The LMRGs were further filtered by intersecting with DEGs between glioma and brain tissue samples in the TCGA dataset.
Li J., Zhang S., Chen S., Yuan Y., Zuo M., Li T., Wang Z, & Liu Y. (2023). Lipid metabolism-related gene signature predicts prognosis and depicts tumor microenvironment immune landscape in gliomas. Frontiers in Immunology, 14, 1021678.
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Publication 2023
Adipocytes Adipokines alpha-Linolenic Acid Anabolism Arachidonic Acid Bile Acids Brain Cholesterol Digestion Ethers Fatty Acids Fatty Acids, Unsaturated Glioma Glycerophospholipids Hormones Linoleic Acid Lipid Metabolism Lipolysis Metabolism Peroxisome Proliferator-Activated Receptors Signal Transduction Pathways Sphingolipids Steroids Tissues

Top products related to «Sphingolipids»

1
Absoluteidq p180 kit by Biocrates
Sourced in Austria, United States, Japan, Germany
The AbsoluteIDQ p180 kit is a targeted metabolomics assay developed by Biocrates. The kit provides a quantitative analysis of up to 188 metabolites from various chemical classes, including acylcarnitines, amino acids, biogenic amines, and lipids. The kit utilizes flow injection analysis-tandem mass spectrometry (FIA-MS/MS) technology to enable the simultaneous measurement of these metabolites in a single analysis.
2
4000 qtrap by AB Sciex
Sourced in United States, Canada, Germany, Japan
The 4000 QTRAP is a highly sensitive and versatile mass spectrometry system designed for quantitative and qualitative analysis. It combines a triple quadrupole and a linear ion trap in a single instrument, providing enhanced performance and functionality for a wide range of applications.
3
Sphingolipid standards by Avanti Polar Lipids
Sourced in United States
Sphingolipid standards are reference materials used to identify and quantify various types of sphingolipids in biological samples. They serve as a benchmark for analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry.
4
Analyst 1 by AB Sciex
Sourced in United States, Canada, Germany, Singapore, United Kingdom
Analyst 1.6.3 software is a data processing and analysis tool developed by AB Sciex for use with their mass spectrometry instruments. The software provides functionalities for data acquisition, processing, and visualization. It serves as a core component in the operation and analysis of mass spectrometry experiments.
5
Tsq 7000 by Thermo Fisher Scientific
Sourced in Germany, United States
The TSQ 7000 is a high-performance triple quadrupole mass spectrometer designed for quantitative and qualitative analysis in various applications. The instrument provides reliable and accurate measurements with its advanced technology and robust design.
6
Analyst 1 by Thermo Fisher Scientific
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Analyst 1.5 software is a data analysis tool designed for use with Thermo Fisher Scientific's analytical instruments. The software provides users with functions to collect, process, and analyze data generated from these instruments.
7
Qtrap 5500 mass spectrometer by AB Sciex
Sourced in United States, Canada, Japan, Germany, Austria, China, Switzerland
The QTRAP 5500 mass spectrometer is a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. It is designed to provide sensitive and selective analysis of small molecules. The instrument features a triple quadrupole configuration and advanced ion optics for efficient ion transmission and fragmentation.
8
Metidq software by Biocrates
Sourced in Austria, Japan, United States
MetIDQ software is a comprehensive data analysis solution designed for metabolomics research. It provides automated data processing, statistical analysis, and visualization tools to support the interpretation of metabolomics data.
9
Acquity uplc system by Waters Corporation
Sourced in United States, United Kingdom, Germany, France, Japan, Singapore, China, Ireland
The Acquity UPLC system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. The system utilizes ultra-high performance liquid chromatography (UPLC) technology to provide rapid and efficient separation of complex samples. The Acquity UPLC system is capable of operating at high pressures and flow rates, enabling the use of small particle size columns to achieve enhanced chromatographic resolution and sensitivity.
10
C17 sphingosine by Avanti Polar Lipids
Sourced in United Kingdom, United States
C17-sphingosine is a long-chain sphingoid base used as an internal standard for the analysis of sphingolipids. It serves as a reference compound for the identification and quantification of related lipid species.

More about "Sphingolipids"

Sphingolipids are a diverse class of lipids that play crucial roles in cellular signaling, membrane structure, and a wide range of biological processes.
These complex molecules are composed of a sphingoid base, a long-chain amino alcohol, and various headgroup moieties.
Dysregulation of sphingolipid metabolism has been implicated in numerous diseases, including neurodegenerative disorders, cancer, and metabolic conditions.
Researchers studying sphingolipids have a wide range of analytical tools and techniques at their disposal.
The AbsoluteIDQ p180 kit, for example, enables the quantification of over 180 metabolites, including several sphingolipid species.
The 4000 QTRAP and QTRAP 5500 mass spectrometers provide high-sensitivity and high-resolution analysis of sphingolipids, while the Analyst 1.6.3 and Analyst 1.5 software packages facilitate data processing and interpretation.
In addition to analytical instrumentation, researchers can leverage a variety of sphingolipid standards and internal standards, such as C17-sphingosine, to ensure accurate quantification and identification of these important biomolecules.
The MetIDQ software, designed for metabolomics data analysis, can also be a valuable tool for exploring the complexities of sphingolipid metabolism.
Optimizing your sphingolipid research can be a challenge, but PubCompare.ai's AI-driven platform can help.
The tool enables you to discover, compare, and identify the best published methods, pre-prints, and patents, allowing you to enhance reproducibility and accelerate your work.
By navigating the literature and identifying top products and experimental approaches, PubCompare.ai can streamline your research and help you uncover new insights into the mechanisms and functions of these fascinating lipids.
OtherTerms: Sphingoid base, amino alcohol, headgroup moieties, AbsoluteIDQ p180 kit, 4000 QTRAP, QTRAP 5500, Analyst 1.6.3, Analyst 1.5, C17-sphingosine, MetIDQ software, Acquity UPLC system, pre-prints, patents, reproducibility, metabolomics