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Staurosporine

Staurosporine is a potent kinase inhibitor with a wide range of biological activities.
It is commonly used in research to study cell signaling pathways and induce apoptosis.
Staurosporine has been investigated for its potential therapeutic applications in cancer, neurodegeneration, and other diseases.
This MeSH term provides a comprehensive overview of the properties, uses, and research related to this important pharmacological tool.

Most cited protocols related to «Staurosporine»

1
Quantifying Cell Colony Formation Assay
Cited 139 times
2500 cells per well were plated in 12-well plates (Greiner Bio One Cellstar, Frickenhauser - Germany) and were allowed to grow for about 4 to 5 days until small colonies could be clearly seen. Cells were treated for 48 hrs with different concentrations (2–100 nM) of staurosporine or UCN-01 (7-hydroxystaurosporine) in growth media. For each concentration datapoint of the two drugs, cells were analyzed in quadruplicates. Staurosporine was purchased as 1 mM ready-made solution in DMSO (Sigma Cat # S6942) and UCN-01 as powder (Sigma Cat # U6508). UCN-01 was diluted in DMSO according to the manufacturer's instructions. Cell culture plates containing colonies were gently washed with PBS and fixed with 3.7% formaldehyde for 10 minutes. Wells were rinsed once again with PBS and colonies were stained with 0.2% crystal violet solution in 10% ethanol for 10 minutes. Excess stain was removed by washing repeatedly with PBS. All the procedures were done at room temperature. The plates can be stored at room temperature or at +4 °C for several months without any visible fading of the dye.
Guzmán C., Bagga M., Kaur A., Westermarck J, & Abankwa D. (2014). ColonyArea: An ImageJ Plugin to Automatically Quantify Colony Formation in Clonogenic Assays. PLoS ONE, 9(3), e92444.
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Publication 2014
7-hydroxystaurosporine Cell Culture Techniques Cells Culture Media Ethanol Formaldehyde Pharmaceutical Preparations Powder Stains Staurosporine Sulfoxide, Dimethyl UCN 01 Violet, Gentian Vision
2
Structural and Functional Activation of BAX
Cited 37 times
SAHBs were synthesized using our established method46 (link),47 (link) and recombinant BAX for NMR and biochemical analyses was generated as previously described33 (link),35 (link). Samples for HSQC and PRE NMR contained uniformly 15N-labeled BAX at 0.2 mM prepared in 10 mM sodium acetate solution at pH 6.0 with up to a 1:1 molar ratio of SAHB. NMR spectra were acquired at 32°C on Bruker 600 and 800 MHz spectrometers, and then processed and analyzed as described in the Full Methods. To evaluate BIM SAHB-induced BAX activation, four in vitro assays were performed. The oligomerization assay employed freshly purified monomeric BAX in combination with BIM SAHB at the indicated ratios and incubation durations followed by size-exclusion chromatography to quantify monomeric vs. oligomeric BAX. The BAX conformational change assay also employed the indicated BIM SAHB:BAX mixtures, which were exposed to the conformation-specific 6A7 anti-BAX antibody, followed by immunoprecipitation and BAX Western analysis to monitor the proportion of activated conformer of BAX upon BIM SAHB exposure. To determine if the BIM SAHB-induced BAX conformational change reflected functional activation of its release activity, we conducted liposomal and mitochondrial release assays as previously described33 (link),48 (link) and using the indicated doses and constructs of BIM SAHB and BAX. For cellular studies, DKO MEFs were reconstituted with BAX by retroviral transduction of BAX-IRES-GFP as previously reported7 (link),13 (link) and as described in the Full Methods. BAX or BAXK21E-reconstituted DKO MEFs were exposed to either BIM SAHBs or staurosporine, and cell death quantified over time by annexin-V-Cy3 staining followed by flow cytometric analysis.
Gavathiotis E., Suzuki M., Davis M.L., Pitter K., Bird G.H., Katz S.G., Tu H.C., Kim H., Cheng E.H., Tjandra N, & Walensky L.D. (2008). BAX Activation is Initiated at a Novel Interaction Site. Nature, 455(7216), 1076-1081.
Publication 2008
Annexin A5 Antibodies, Anti-Idiotypic Biological Assay Cell Death Cells Flow Cytometry Gel Chromatography Immunoprecipitation Internal Ribosome Entry Sites Liposomes Mitochondria Molar Retroviridae Sodium Acetate Staurosporine
3
Structural and Functional Activation of BAX
Cited 36 times
SAHBs were synthesized using our established method46 (link),47 (link) and recombinant BAX for NMR and biochemical analyses was generated as previously described33 (link),35 (link). Samples for HSQC and PRE NMR contained uniformly 15N-labeled BAX at 0.2 mM prepared in 10 mM sodium acetate solution at pH 6.0 with up to a 1:1 molar ratio of SAHB. NMR spectra were acquired at 32°C on Bruker 600 and 800 MHz spectrometers, and then processed and analyzed as described in the Full Methods. To evaluate BIM SAHB-induced BAX activation, four in vitro assays were performed. The oligomerization assay employed freshly purified monomeric BAX in combination with BIM SAHB at the indicated ratios and incubation durations followed by size-exclusion chromatography to quantify monomeric vs. oligomeric BAX. The BAX conformational change assay also employed the indicated BIM SAHB:BAX mixtures, which were exposed to the conformation-specific 6A7 anti-BAX antibody, followed by immunoprecipitation and BAX Western analysis to monitor the proportion of activated conformer of BAX upon BIM SAHB exposure. To determine if the BIM SAHB-induced BAX conformational change reflected functional activation of its release activity, we conducted liposomal and mitochondrial release assays as previously described33 (link),48 (link) and using the indicated doses and constructs of BIM SAHB and BAX. For cellular studies, DKO MEFs were reconstituted with BAX by retroviral transduction of BAX-IRES-GFP as previously reported7 (link),13 (link) and as described in the Full Methods. BAX or BAXK21E-reconstituted DKO MEFs were exposed to either BIM SAHBs or staurosporine, and cell death quantified over time by annexin-V-Cy3 staining followed by flow cytometric analysis.
Gavathiotis E., Suzuki M., Davis M.L., Pitter K., Bird G.H., Katz S.G., Tu H.C., Kim H., Cheng E.H., Tjandra N, & Walensky L.D. (2008). BAX Activation is Initiated at a Novel Interaction Site. Nature, 455(7216), 1076-1081.
Publication 2008
Annexin A5 Antibodies, Anti-Idiotypic Biological Assay Cell Death Cells Flow Cytometry Gel Chromatography Immunoprecipitation Internal Ribosome Entry Sites Liposomes Mitochondria Molar Retroviridae Sodium Acetate Staurosporine
4
Genetic Manipulation of Cell Death Pathways
Cited 24 times
CRISPR-mediated knockout plasmids containing guide RNAs targeting BAX, BAK1, NCKAP1, ACSL4, SLC7A11, CYFIP1, WAVE-2, Abi2, HSPC300 were generated in lentiCRISPR v2 (Addgene, #52961) according to the standard protocol. The SLC7A11 cDNA–containing expression construct was described in previous publications25 , 26 . The lentiviral construct expressing membrane-bound green fluorescent protein (mGFP) (#22479) and Rac1-Q61L cDNA-containing construct (#84605) were obtained from Addgene. NCKAP1 cDNA and shRNA constructs targeting RPN1, N-WASP, WHAMM were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. NCKAP1 and Rac1-Q61L cDNA were subsequently cloned into the vector pLX302 with a C-terminal V5 tag (Addgene, #25896). WAVE-2 constructs were provided by Dr. Daniel D. Billadeau. All constructs were confirmed by DNA sequencing. The sequences of gRNAs and shRNA used in this study are listed in Supplementary Table 4. Necroptosis inhibitor Nec-1s (#2263) was from BioVision, and necrosis inhibitor Necrox-2 (#ALX-430-166-M001) was from Enzo. Ferroptosis inducer (1S,3R)-RSL3 (#19288) and apoptosis inducer staurosporine (#81590) were from Cayman Chemical. L-[1, 2, 1', 2'-14C]-cystine (#NEC854010UC) was from PerkinElmer. KL-11743 was from Kadmon. The following reagents were obtained from Sigma-Aldrich: 2-deoxy-D-glucose (#D8375-1G), Trolox (#238813), 4-Hydroxy-TEMPO (Tempol) (#176141), beta-mercaptoethanol (2ME) (#M6250), deferoxamine mesylate salt (DFO) (#D9533), ferrostatin-1 (#SML0583), chloroquine (#C6628), diamide (#D3648), diethyl-maleate (#D97703, BAY-876 (#SML1774), and L-Cystine (#C7602). All reagents were dissolved according to manufacturers’ instructions.
Atkin W.S., Hart A., Edwards R., Cook C.F., Wardle J., McIntyre P., Aubrey R., Baron C., Sutton S., Cuzick J., Senapati A, & Northover J.M. (2000). Single blind, randomised trial of efficacy and acceptability of oral Picolax versus self administered phosphate enema in bowel preparation for flexible sigmoidoscopy screening. BMJ : British Medical Journal, 320(7248), 1504-1509.
Publication 2023
2-Mercaptoethanol ABI2 protein, human Apoptosis BAK1 protein, human BAY-876 Caimans Chloroquine Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats Cystine Diamide diethyl maleate DNA, Complementary Ferroptosis ferrostatin-1 Glucose Malignant Neoplasms Membrane Proteins Mesylate, Deferoxamine NCKAP1 protein, human Necroptosis Necrosis oxytocin, 1-desamino-(O-Et-Tyr)(2)- Plasmids RNA Salts Short Hairpin RNA Staurosporine tempol TEMPOL-H Trolox C WASL protein, human
5
Dissecting HIV Infection Dynamics
Cited 19 times

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Publication 2008
Blood CD4 Positive T Lymphocytes Cells HIV Infections Infection jasplakinolide latrunculin A Marshes Peptides Pertussis Toxin Staurosporine

Most recents protocols related to «Staurosporine»

1
MEK Inhibitor Sensitivity Across Cancer Lines
Not available on PMC !

Example 20

240 cell lines representative of multiple cancer indications with known alterations in the MAPK pathway, including KRAS, NRAS, HRAS, NF1, EGFR, BRAF and CRAF mutations, were seeded overnight in 386-well plates, then treated with a 9-point dose response of exemplary MEK inhibitors (starting dose of 100 nM and 3-fold dilution) for 5 days. Cell viability was determined using a Cell Titer Glo (CTG) assay. Percent inhibition was calculated for all compounds utilizing staurosporine (1000 nM) treatment as a measure of maximal inhibition. IC50 and area under the curve (AUC) values were determined by fitting a variable slope, four parameters curve to the compound concentration to percent inhibition relationship.

Compared to RAS/RAF wild-type cell lines, increased sensitivity to MEK inhibitors, such as I-2, was observed in cell lines with KRAS, NRAS, BRAF Class I and III mutations, as well as CRAF-alterations (both CRAF mutations and fusions). Cell lines with mutations in PIK3CA, PTEN, NF1, EGFR and HRAS showed similar sensitivity to MEK inhibition to RAS/RAF wild-type cell lines.

US11878958B2. MEK inhibitors and uses thereof (2024-01-23). Ikena Oncology, Inc. [US]. Inventors: Alfredo C. Castro [US], Michael J. Burke [US], Thomas A. Wynn [US], Sabine K. Ruppel [US], Sergio L. Santillana Soto [US], Eric Haines [US], Lan Xu [US], Oksana Zavidij [US].
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Patent 2024
Biological Assay BRAF protein, human Cell Lines Cells Cell Survival EGFR protein, human HRAS protein, human Hypersensitivity inhibitors K-ras Genes Malignant Neoplasms Mutation NRAS protein, human PIK3CA protein, human Psychological Inhibition PTEN protein, human Raf1 protein, human Staurosporine Technique, Dilution
2
Cell Viability Screening of FDA-Approved Compounds
A total of 313 compounds (approved by the FDA, USP, JP, CFDA, INN, JAN, PMDA, Canada, BAN, EMA, EP, BJP, BP, INN, USAN, Poland) targeting a variety of signaling pathways, including DNA damage, angiogenesis, metabolism, and epigenetics, were tested in two cell lines by the CCK8 assay. Briefly, the cells were seeded in 384-well culture plates at a density of 1000 cells/well in 40 μL media and incubated overnight. Different drugs were added at concentrations of 10, 2, 0.4, 0.08, and 0.016 μM, and 10 μM staurosporine was used as the positive control. After culturing for 72 h, 5 μL CCK8 was added to each well, and the cells were incubated for 1–4 h. The optical density (OD) of the wells was measured at 450 nm, and the inhibition rate of each drug was calculated as (ODS-ODNC)/(ODPC-ODNC) × 100%, where ODS, ODNC, and ODPC correspond to the sample, negative control (DMSO), and positive control, respectively.
Zhang J., Guo F., Li C., Wang Y., Wang J., Sun F., Zhou Y., Ma F., Zhang B, & Qian H. (2023). Loss of TTC17 promotes breast cancer metastasis through RAP1/CDC42 signaling and sensitizes it to rapamycin and paclitaxel. Cell & Bioscience, 13, 50.
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Publication 2023
angiogen Biological Assay Cell Lines Cells DNA Damage Epigenetic Process fluoromethyl 2,2-difluoro-1-(trifluoromethyl)vinyl ether Metabolism n-octadecylphosphocholine Pharmaceutical Preparations Psychological Inhibition Signal Transduction Pathways Staurosporine Sulfoxide, Dimethyl Vision
3
Mitochondrial Membrane Potential Apoptosis Assay
Loss of MMP as a marker of apoptosis was assessed with JC-1 dye (MitoProbe JC-1 Assay Kit, M34152), a cationic carbocyanine dye that accumulates in mitochondria of cells in proportion to MMP. After treatment of the BL cell lines with rituximab in vitro, the cells were harvested, washed, and resuspended in 200 µl of warm medium and stained with JC-1 dye as per the manufacturer’s recommendations. Briefly, cells were resuspended at a density of 1 × 106 cells/ml in warm complete RPMI medium and stained with JC-1 dye at a final concentration of 2 µM for 30 min at 37°C, and analyzed by flow cytometry. Staurosporine (1 µM) was used as a positive control.
Saikumar Lakshmi P., Oduor C.I., Forconi C.S., M’Bana V., Bly C., Gerstein R.M., Otieno J.A., Ong’echa J.M., Münz C., Luftig M.A., Brehm M.A., Bailey J.A, & Moormann A.M. (2023). Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapies. Life Science Alliance, 6(5), e202101355.
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Publication 2023
Aftercare Apoptosis Biological Assay Carbocyanines Cations Cell Lines Cells Flow Cytometry Mitochondria Rituximab Staurosporine
4
Comprehensive Antibody Validation Protocol
Anti-CDC42 (#10155-1-AP, 1:1000 for WB), anti-PAK4 (#14685-1-AP, 1:1000 for WB), anti-PAK1(#21401-1-AP, 1:1000 for WB), monoclonal anti-GST(#66001-2-Ig, 1:10000 for WB), polyclonal anti-HA (#51064-2-AP, 1:5000 for WB), monoclonal anti-SIRT2 (#66410-1-IG, 1:10000 for WB), anti-beta tubulin (#10094-1-AP, 1:2000 for WB), MMP-2 (#10373-2-AP, 1:1000 for WB), MMP-9 (#10375-2-AP, 1:1000 for WB) were purchased from Proteintech; Monoclonal anti-HA (#AB0025, 1:5000 for WB); E-cadherin (#BS1098, 1:1000 for WB) were from Bioworld; Monoclonal anti-CDC42 (#sc-8401, 2 μg per 500 μg of total protein for Immunoprecipitation), anti-p-PAK4 (sc-135775, 1:200 for WB) were from Santa Cruz; Anti-p-PAK1 (#2601, 1:1000 for WB), anti-p-AKT (#4060, 1:1000 for WB), anti-AKT (#4691, 1:1000 for WB), anti-p-ERK (#9101, 1:1000 for WB), anti-ERK (#4370, 1:1000 for WB), anti-p-p38 (#9211, 1:1000 for WB), anti-p38 (#9212, 1:1000 for WB), anti-p-JNK (#9251, 1:1000 for WB), anti-ERK (#9252, 1:1000 for WB), anti-caspase 3 (#14220, 1:1000 for WB) and anti-PARP (#9542, 1:1000 for WB) were from Cell Signaling Technology; Monoclonal anti-HA (#M180, 1:500 for Immunoprecipitation), monoclonal anti-Flag (#M185, 1:500 for Immunoprecipitation), polyclonal anti-Flag (#PM020, 1:1000 for WB) and deacetylase inhibitors TSA (#9950) were from MBL; Deacetylase inhibitor NAM (#N1651) was from APExBIO; CBP/p300 inhibitor A-485 (#N1651) was from MCE; SIRT2 inhibitor AK7 (#4754–10) was from Tocris Bioscience; Staurosporine (#abs810006) was from Absin; Polybrene (#H9268) and puromycin (#P8833) were from Sigma-Aldrich. The anti-CDC42 K153Ac specific polyclonal antibody was raised against a synthetic peptide (Jiaxuan Biotech). In brief, the immune peptide DLKAVK(Ac)YVECSALTQKGL was used as antigen to immunize rabbits. During 45 days, rabbits were immunized for four times, and the antiserum was collected, and control peptide DLKAVKYVECSALTQKGL was used to remove non-specific antibody. The sensitivity and specificity of antibody were evaluated by ELISA and WB.
Wang D.N., Ni J.J., Li J.H., Gao Y.Q., Ni F.J., Zhang Z.Z., Fang J.Y., Lu J, & Yao Y.F. (2023). Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation. PLOS Pathogens, 19(2), e1011189.
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Publication 2023
Antibodies, Anti-Idiotypic Antigens beta-Tubulin Caspase 3 CDC42 protein, human CDH1 protein, human Enzyme-Linked Immunosorbent Assay EP300 protein, human Immune Sera Immunoglobulins Immunoprecipitation inhibitors MMP2 protein, human MMP9 protein, human Oryctolagus cuniculus PAK1 protein, human PAK4 protein, human Peptides Polybrene Proteins Puromycin SIRT2 protein, human Staurosporine
5
Macrophage Apoptosis Modulation by MoxR1
In a 24-well plate, RAW264.7 (0.5 × 106) macrophage cells were plated. 4 µg/ml of MoxR1 was used to treat cells for 24 hrs at 37 °C. A positive control involving RAW264.7 cells was treated with 0.1 µM staurosporine (Sigma, USA) for 24 hrs. The ZVAD-FMK (20 µM) was used as a caspase inhibitor and served as the control for apoptosis inhibition. Untreated cells and cells treated with HI MoxR1 (4 µg/ml) served as the negative controls. The Annexin V/PI kit was used in detecting apoptosis. BD FACS Verse Flow Cytometer was used to measure the fluorescent intensity of the stained samples. Following sample reading acquisition, cells were examined using the FlowJo software.
Quadir N., Shariq M., Sheikh J.A., Singh J., Sharma N., Hasnain S.E, & Ehtesham N.Z. (2023). Mycobacterium tuberculosis protein MoxR1 enhances virulence by inhibiting host cell death pathways and disrupting cellular bioenergetics. Virulence, 14(1), 2180230.
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Publication 2023
Anastasis Annexin A5 Apoptosis benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone Caspase Inhibitors Cells Macrophage RAW 264.7 Cells Staurosporine

Top products related to «Staurosporine»

1
Staurosporine by Merck Group
Sourced in United States, Germany, United Kingdom, France, Italy, Sao Tome and Principe, Israel, Canada, Austria, Switzerland
Staurosporine is a small molecule compound that acts as a broad-spectrum protein kinase inhibitor. It is commonly used as a research tool in cell biology and biochemistry studies.
2
Staurosporine by Cell Signaling Technology
Sourced in United States
Staurosporine is a small molecule compound that acts as a broad-spectrum protein kinase inhibitor. It has been widely used as a research tool to study cellular signaling pathways involving protein phosphorylation.
3
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
4
Caspase glo 3 7 assay by Promega
Sourced in United States, Germany, United Kingdom, Switzerland, Italy, France, Spain, Japan
The Caspase-Glo 3/7 Assay is a luminescent-based assay that measures the activities of caspase-3 and caspase-7, two key enzymes involved in the execution phase of apoptosis. The assay utilizes a luminogenic caspase-3/7 substrate, which, upon cleavage by the enzymes, generates a glow-type luminescent signal that is proportional to the amount of caspase activity present in the sample.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Etoposide by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, France, China, Italy, Canada, Switzerland, Belgium, Macao, Portugal, Poland
Etoposide is a chemotherapeutic agent used in the treatment of various types of cancer. It is a topoisomerase inhibitor that disrupts the process of DNA replication, leading to cell death. Etoposide is available as a solution for intravenous administration.
7
Staurosporine by Selleck Chemicals
Sourced in United States, Germany
Staurosporine is a chemical compound commonly used as a laboratory reagent. It is a potent and broad-spectrum protein kinase inhibitor, capable of affecting a wide range of cellular processes. Staurosporine is often utilized in research settings to study the role of protein kinases in various biological systems.
8
Staurosporine by Abcam
Sourced in United Kingdom, Germany, United States
Staurosporine is a natural product isolated from the bacterium Streptomyces staurosporeus. It is a potent inhibitor of various protein kinases, including protein kinase C (PKC), and has been widely used as a tool compound in cell biology research.
9
Cycloheximide (chx) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, Japan, Spain, Canada, Switzerland, India, Israel, Brazil, Poland, Portugal, Australia, Morocco, Sweden, Austria, Senegal, Belgium
Cycloheximide is a laboratory reagent commonly used as a protein synthesis inhibitor. It functions by blocking translational elongation in eukaryotic cells, thereby inhibiting the production of new proteins. This compound is often utilized in research applications to study cellular processes and mechanisms related to protein synthesis.
10
Staurosporine st by Merck Group
Sourced in United States, Germany, Canada
Staurosporine (STS) is a compound that inhibits a variety of protein kinases. It is commonly used as a laboratory tool in cell biology and biochemical research. STS functions as a broad-spectrum protein kinase inhibitor, affecting the activity of various enzymes involved in cellular signaling pathways.

More about "Staurosporine"

Staurosporine (STS) is a potent and versatile pharmacological tool that has garnered significant attention in the scientific community.
This indolocarbazole compound is a highly selective and broad-spectrum protein kinase inhibitor, with a wide range of biological activities.
Staurosporine has been extensively utilized in research to study various cell signaling pathways and to induce programmed cell death, or apoptosis, in a variety of cell types.
One of the key applications of Staurosporine is in cancer research, where it has been investigated for its potential therapeutic applications.
By targeting and inhibiting multiple kinases, Staurosporine can disrupt the signaling cascades that are critical for cancer cell survival and proliferation.
Additionally, Staurosporine has shown promise in the study of neurodegenerative disorders, where it may play a role in protecting against neuronal cell death.
Researchers often use Staurosporine in conjunction with other pharmacological agents, such as DMSO (Dimethyl Sulfoxide), which is commonly used as a solvent for Staurosporine.
The Caspase-Glo 3/7 Assay is another valuable tool that can be used to measure the apoptotic response induced by Staurosporine treatment.
Fetal Bovine Serum (FBS) may also be used in cell culture experiments to provide necessary growth factors and nutrients for cells exposed to Staurosporine.
Other related compounds, such as Etoposide and Cycloheximide, have also been studied in the context of Staurosporine-induced apoptosis, as they can modulate the cellular response to this potent kinase inhibitor.
By understanding the interactions and synergies between these various agents, researchers can optimize their experimental protocols and enhance the reproducibility and accuracy of their Staurosporine-based studies.
In summary, Staurosporine is an invaluable tool in the field of cell biology and pharmacology, with a broad range of applications and a growing body of research supporting its potential therapeutic uses.
By leveraging the insights gained from the extensive literature on Staurosporine, researchers can design more effective and efficient experiments, leading to a deeper understanding of cellular signaling, apoptosis, and the development of novel therapeutic interventions.