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Sulfate, Atropine

Sulfate and atropine are two important compounds in biomedical research.
Sulfate is an anion that plays a crucial role in various physiological processes, while atropine is a tropane alkaloid with anticholinergic properties.
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Most cited protocols related to «Sulfate, Atropine»

CFB−/− mice on a C57BL/6 background were generously provided by V. Michael Holers (University of Colorado Health Science Center, Denver, CO). C57BL/6 mice were generated from breeding pairs (Harlan Laboratories, Indianapolis, IN). Animals were housed under a 12-hour light/12-hour dark cycle with access to food and water ad libitum.
For CNV lesions, 3-month-old mice were anesthetized (20 mg/kg xylazine, 80 mg/kg ketamine), and pupils were dilated (2.5% phenylephrine HCl and 1% atropine sulfate). Argon laser photocoagulation (532 nm, 100 μm spot size, 0.1 second duration, 100 mW) was used to generate four laser spots in each eye surrounding the optic nerve with the use of a handheld coverslip as a contact lens. Bubble formation at the laser spot indicated the rupture of Bruch membrane.17 (link)
For tail-vein injections, the vein was vasodilated by heat, a 25-gauge needle was inserted, and a 50 μL volume was injected (250 μg CR2-fH in PBS, molar equivalent of CR2 in PBS [113 μg], or PBS only). Dosing and treatment schedules are outlined in Results. All experiments were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and were approved by the University Animal Care and Use Committee.
Publication 2009
Animals Argon Ion Lasers Bruch Membrane Contact Lenses Exanthema Food Ketamine Light Coagulation Mice, House Mice, Inbred C57BL Molar Needles Optic Nerve Phenylephrine Hydrochloride Pupil Sulfate, Atropine Tail Treatment Protocols Veins Vision Xylazine
2 h before cremaster muscle exteriorization, mice received 4 μg PTx i.v. (Sigma-Aldrich) and 500 ng TNF-α intrascrotally (R&D Systems). Mice were anesthetized with an i.p. injection of 125 mg/kg ketamine hydrochloride (Sanofi), 0.025 mg/kg atropine sulfate (Fujisawa), and 12.5 mg/kg xylazine (TranquiVed; Phoenix Scientific) and placed on a heating pad. The cremaster muscle was prepared as previously described (51 (link)). Postcapillary venules with a diameter between 20 and 40 μm were recorded using an intravital microscope (Axioskop, SW 40/0.75 objective; Carl Zeiss, Inc.) through a digital camera (sensicam qe; Cooke Corporation). Blood flow centerline velocity was measured using a dual-photodiode sensor system (CircuSoft Instrumentation).
Publication 2008
Blood Flow Velocity Cremaster Muscle Fingers Intravital Microscopy Iodine-125 Ketamine Hydrochloride Mice, House Sulfate, Atropine Tumor Necrosis Factor-alpha Venules Xylazine
Adult male cats (ages: 6–12 months, n =40) were tranquilized with acepromazine (0.2 mg/kg, intramuscularly) and initially anesthetized with ketamine (10 mg/kg, intramuscularly). An intravenous catheter was inserted into each hind limb to allow continuous infusions of propofol (5–6 mg/kg/hr) and sufentanil (10–20 ng/kg/hr) for anesthesia, vecuronium bromide (0.2 mg/kg/hr) for muscle paralysis, and saline (1–3 ml/hr) for hydration. All vital signs were closely monitored and carefully maintained within normal physiological limits. The nictitating membranes were retracted with 2% neosynephrine and the pupils dilated with 1% atropine sulfate. Contact lenses were used to protect the corneas and focus visual stimuli on the retina. The positions of the optic disk and the area centralis were plotted on a screen in front of the animal by using a fiber optic light source. Details of the surgical procedures have been described previously10 (link). We also performed recordings in one male rhesus macaque (age: 8.5 years, 10 Kg) using similar procedures to those described above. The macaque was anesthetized with ketamine (10 mg/kg, intramuscularly) and diazepam (0.75 mg/kg, intravenous) followed by propofol (1.8 mg/kg, intravenous) and a continuous infusion of sufentanil citrate that was maintained throughout the experiment (6–20 μg/kg/hr, intravenous). The animal was paralyzed after finishing the surgery with vecuronium bromide (0.1 mg/kg/hr, intravenous).
Publication 2016
Acepromazine Adult A Fibers Anesthesia Animals Catheters Citrate, Sufentanil Contact Lenses Cornea Diazepam Eye Felis catus Hindlimb Ketamine Light Macaca Macaca mulatta Males Neo-Synephrine Neuromuscular Block Nictitating Membrane Operative Surgical Procedures Optic Disk physiology Propofol Pupil Retina Saline Solution Signs, Vital Sufentanil Sulfate, Atropine Vecuronium Bromide
To stimulate endogenous release of norepinephrine, all rats were infused for 15 min with tyramine (1.26 μmol/min/kg, 217 μl/min/kg, Berg, 2005 (link)). The control group (PBS + tyramine) was injected with PBS 10 min prior to the tyramine-infusion. To identify responses caused by the tyramine-induced reverse transport through NET, rats were injected i.p. with the NET inhibitor desipramine hydrochloride (44 μmol/kg) 5 h prior to the experiment (Miralles et al., 2002 (link); Berg et al., 2012 (link)), and pre-treated with PBS 10 min before tyramine during the experiment (desipramine + PBS + tyramine). To test if the tyramine-evoked rise in BP elicited baroreceptor activation and reflex vagal inhibition of HR, another group was pre-treated with the muscarinic receptor antagonist atropine sulfate (2.9 μmol, Berg, 2002 (link)) 20 min before tyramine (atropine + tyramine). To analyze the influence of α2AR, rats were pre-treated with the non-selective α2AR antagonist L-659,066, which does not penetrate the blood-brain barrier (Clineschmidt et al., 1988 (link); 4.4 μmol/kg, 10 min before tyramine, Berg et al., 2012 (link); L-659,066 + tyramine), or with the non-selective, α2AR-agonist clonidine, which easily penetrates the blood-brain barrier (151 nmol/kg, 15 min prior to tyramine, Berg et al., 2012 (link)). Clonidine was injected 10 min after a sham-injection with PBS (PBS + clonidine + tyramine) or L-659,066 as above (L-659,066 + clonidine + tyramine) to differentiate between involvement of CNS and peripheral α2AR. In a time-control group, the rats were pre-treated with PBS and subsequently infused with PBS instead of tyramine (PBS + PBS). The number of rats per group is shown in Table 1.
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Publication 2013
Atropine Blood-Brain Barrier Clonidine Desipramine Hydrochloride, Desipramine L 659066 Muscarinic Antagonists Norepinephrine Pneumogastric Nerve Pressoreceptors Psychological Inhibition Rattus norvegicus Reflex Sulfate, Atropine Tyramine
Overnight dark-adapted mice (5 weeks of age) were anesthetized with intraperitoneal ketamine and xylazine as described above. Pupils were dilated with 1% (w/v) atropine sulfate in saline solution. The mice were exposed to fluorescent light at 800 lux for 5 min, then transferred to darkness for the indicated times. Mice were killed by cervical dislocation under anesthesia, eyes were enucleated, and the retinas and RPE were separately dissected. All tissue manipulations were done under dim red light (Kodak Wratten 1A). The tissues were homogenized in PBS and the retinoids extracted into hexane as described previously (Radu et al., 2008b (link)). For the ex vivo experiment, we isolated retinas from dark-adapted, 5-week-old wild-type and irbp−/− mice and placed each in 100 µl of conditioned DMEM culture medium plus 20 mm HEPES buffer, pH 7.2. The wild-type retinal explants were placed in conditioned medium from 293T cells expressing human IRBP, while the irbp−/− explants were placed in conditioned medium from 293T cells transfected with nonrecombinant pRK5 plasmid. The retinas were exposed to white light at 800 lux for 5 min, then incubated in the dark for 0, 15, 30, or 60 min. We collected the retinas by centrifugation and homogenized them in 20 mm HEPES, pH 7.2, 0.1% SDS and 150 mm hydroxylamine. An equal volume of ethanol was added and the retinoids were extracted into hexane for HPLC analysis. Retinoids were analyzed by normal-phase HPLC as described previously (Radu et al., 2008b (link)). In brief, samples were dissolved in 200 µl of hexane, and retinoids were separated by chromatography on a silica column (Zorbax-Sil 5 µm, 250 × 4.6 mm, Agilent Technologies) by gradient elution of mobile phase (0.2–10% dioxane in hexane, 2ml per min flow rate) in an Agilent 1100 liquid chromatograph equipped with a UV photodiode-array detector. Identified peaks were confirmed by spectral analysis and coelution with authentic retinoid standards.
Publication 2009
Anesthesia Buffers Centrifugation Chromatography Culture Media, Conditioned Darkness Dioxanes Ethanol Eye HEK293 Cells HEPES High-Performance Liquid Chromatographies Homo sapiens Hydroxylamine Joint Dislocations Ketamine Light Liquid Chromatography Mus n-hexane Neck Plasmids Pupil Retina Retinoids Saline Solution Silicon Dioxide Sulfate, Atropine Tissues Xylazine

Most recents protocols related to «Sulfate, Atropine»

In addition to the recordings provided by the hybrid diffuse optical setup, SpO2 , heart rate (HR) and mean arterial pressure (MAP) were extracted from either an anesthesia monitor (Datex-Ohmeda Aisys™, GE Healthcare, Little Chalfont, United Kingdom) by the open-source VitalSigns capture (VScapture) program60 (link) or from a general monitor (Philips IntelliVue MX800, Koninklijke Philips N.V.). The BIS data were acquired by a BIS sensor (BIS Vista™, Medtronic plc, IRL). These signals were recorded by the same monitor with the same timestamps and were synchronized with the optical signals with a precision of 1 s and according to the beginning of the measurement.
General anesthesia was performed under total intravenous anesthesia with propofol at a concentration of 1% (Propofol Fresenius®, Fresenius Kabi Deutschland GmbH, Bad Homburg, Germany) using the Schneider model of target-controlled infusion anesthesia61 (link) based on age, height, weight, and gender of the patient3 ,62 (link) implemented in a TIVA system (Alaris Asena® PK, Becton, Dickinson and Company, Franklin Lakes, New Jersey). After induction and tracheal intubation, pulmonary volume-controlled ventilation was maintained with a fraction of inspired oxygen ( FiO2 ) of 0.5, a tidal volume of 6 to 7  mg/kg , a respiration rate between 12 and 16 breaths per minute, a positive end-expiratory pressure of 4 to 6 mmHg, and a MAP between 60 and 80 mmHg.
It should be noted that the patients received further medications, such as fentanyl for analgesia,63 (link)65 (link, link) atropine sulfate, atracurium besylate, or rocuronium bromide to obtain neuromuscular blockade, remifentanil, neostigmine, lidocaine, and midazolam. Some of them are known to influence cerebral hemodynamics (i.e., fentanyl,63 (link)65 (link, link) midazolam,66 (link) remifentanil,67 (link) atropine sulfate,68 (link) and lidocaine69 (link)). Our study is not designed to investigate these interactions and it is not trivial to consider these effects since they are not well known. Since the primary goal of the study the direct comparison of the optical and BIS signals, we have decided to remove the duration of their administration from all the signals as described in the following section.
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Publication 2023
Anesthesia Anesthesia, Intravenous Atracurium Besylate Fentanyl General Anesthesia Hemodynamics Hybrids Intubation, Intratracheal Lidocaine Management, Pain Midazolam Neostigmine Neuromuscular Block Oxygen Patients Pharmaceutical Preparations Positive End-Expiratory Pressure Propofol Propofol Fresenius Rate, Heart Remifentanil Respiratory Rate Rocuronium Bromide Saturation of Peripheral Oxygen Sulfate, Atropine Tidal Volume Vision
Mice were anesthetized with an intraperitoneal injection of a mixture of Fentanyl (0.05 mg/kg bodyweight (BW), Fentanyl-Hameln, Hameln Pharma plus, Hameln, Germany), Midazolam (5.0 mg/kg BW, Midazolam-hameln®; Hameln Pharma plus), Medetomidin (0.5 mg/kg BW, Sedator®; Albrecht, Aulendorf, Germany) and atropine sulfate (0.2 mg/kg BW, B. Braun, Melsungen, Germany) diluted with water ad. inj. (Ampuwa, Fresenius KABI, Bad Homburg, Germany) to an injection volume of 10 mL/kg BW. Additional doses of anesthetics were administered if needed. The anesthesia was antagonized after the measurements by a subcutaneously administered mixture of Naloxon (1.2 mg/kg BW, Naloxon-hameln®; Hameln Pharma plus), Flumazenil (0.55 mg/kg BW, Flumazenil®; Fresenius KABI), and Atipazemol (2.5 mg/kg BW, Antisedan®; VETOQUINOL GmbH, Ravensburg, Germany) diluted with water ad. inj. (Ampuwa) to an injection volume of 10 mL/kg BW.
The anesthetized mice lay on a pre-warmed resting pad (37°C) in a soundproof chamber (IAC 400-A, Industrial Acoustics Company GmbH, Niederkrüchten, Germany). Auditory brainstem responses (ABRs) in anesthetized mice were evoked by short-duration sound stimuli with the same stimulus parameters for all groups. The ABRs represent the summed activity of neurons in distinct anatomical structures along the ascending auditory pathway recorded from subcutaneous cranial electrodes. A microphone (Bruel & Kjaer 4191, Naerum, Denmark) was used to calibrate and record the acoustic stimuli. ABR thresholds were elicited with click stimuli (100 μs duration with an FFT mean of 5.4 kHz). The stimulus level was increased stepwise from 10 to 100 dB SPL in 5 dB steps. Stimuli were generated with an I-O-card (PCI-6052E, PCI-6251, or PCIe-6259, National Instruments, Austin, Texas, United States) in an IBM compatible computer. The SPL of the stimuli was modulated by custom-made amplifier and attenuator systems (Wulf Elektronik, Frankfurt, Germany). The measured signals were band-pass filtered from 200 Hz to 5 kHz (F1, 6-pole Butterworth hardware Filter, Wulf Elektronik) and amplified by 100,000. The analog/digital (A/D) rate was 20 kHz. Each stimulus had a recording interval of 16 ms and was directly repeated and averaged up to 512 times.
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Publication 2023
Acoustics Anesthesia Anesthetics Auditory Brainstem Responses austin Body Weight Cranium Fentanyl Flumazenil Injections, Intraperitoneal Mice, House Midazolam Neurons Pathway, Auditory Sound Strains Sulfate, Atropine
A total of six mini-pigs (Sus scrofa; Medi Kinetics Co. Ltd., Pyeongtaek, Korea) were used for the in vivo experimental study (Supplementary Figure 1). The mean age of the mini-pigs were 14 months and each subject weighed approximately 30 kg. All animals were quarantined and acclimated in a vivarium for one week before the experiments. They were kept in specific pathogen-free facilities with complete substrate feeding. All animals fasted overnight and were given only water for 24 hours before the endoscopic procedure. Pre-anesthesia sedation consisted of an intramuscular injection of tiletamine/zolazepam (5mg/kg), xylazine hydrochloride (2mg/kg), and atropine sulfate (0.04 mg/kg). The animals were subsequently intubated, and general anesthesia was achieved with 1.5% isoflurane (Foran®; JW Pharmaceutical Corp., South Korea). During the procedure, heart rate, respiratory rate, and oxygen saturation were monitored continuously. The pigs resumed their usual diet (1000 kcal/day) on the day after the procedure.
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Publication 2023
Anesthesia Animals Diet General Anesthesia Intramuscular Injection Isoflurane Kinetics Oxygen Saturation Pharmaceutical Preparations Pigs Rate, Heart Respiratory Rate Sedatives Specific Pathogen Free Sulfate, Atropine Surgical Endoscopy Sus scrofa Swine, Miniature tiletamine - zolazepam Xylazine Hydrochloride
During the antigen recall part of the study, six months post the final immunization (see Fig. 3), Purified H107 protein (50 μg per animal in 0.15mL) was administrated by intratracheal route (IT) using endoscope, directly inserted into the trachea until the bifurcation of caudal right lung (accessory lobe and caudal lobe). In parallel, purified H107 and control protein antigen (CTH522; composed of unrelated Chlamydia trachomatis-specific antigens) were administrated by intradermal (ID) route consisted of two intradermal injections of 0.1 mL, each containing 20 μg protein antigen per injection the right (H107) or left (control antigen) side of the back of the animal. In vivo recall antigens were diluted in 10mM Tris + 4%glycerol, pH 7.0. Pre-medication was performed using alpha-2 agonist atropine sulfate (0.04mg/kg) before anesthesia of the animals to reduce bronchospasm and mucus production during endoscopic exam. Animals were then sedated using ketamine hydrochloride (5 mg/kg, IM) associated with medetomidine hydrochloride (0.05mg/kg IM). After bronchoscopy administration and sampling, animals are injected with Atipamezol hydrochloride (0.25mg/kg) to induce recovery from anesthesia. The animals were sampled for blood at days 0, 3 and 8. On day 8, bronchoalveolar lavage (BAL) was also performed using 50 mL sterile saline, and skin biopsy were performed at injection sites. Blood cell counts, hemoglobin and hematocrit were determined from EDTA-treated blood using an HMX A/L analyzer (Beckman Coulter).
Dermal scoring (skin induration, DTH) was performed at days 0 and 8 post boost. Dermal scoring of the dosing site included observations and graded scoring for erythema, edema, bleeding, scabbing, fissuring and/or ulceration. Skin biopsies were gathered using 8mm2 punches and put in PBS after fatty tissue was removed. Briefly, skin biopsies were washed using RPMI 37°C and incubated overnight at 37°C in enzymatic solution (RPMI Glutamax+ 5%FCS+1%ATB+DNase 0.02mg/L) with 4 mg/mL Collagenase D. After incubation, supernatant was collected, aliquoted, and stored at −80°C for antibody ELISA assays. The remaining tissue was dissociated and washed before harvesting cells for stimulation for ICS assays.
Publication Preprint 2023
Adrenergic alpha-2 Receptor Agonists Anesthesia Animals Antigens atipamezole Biological Assay Biopsy BLOOD Blood Cell Count Bronchoalveolar Lavage Bronchoscopy Bronchospasm Cells Chlamydia trachomatis Collagenase Deoxyribonucleases Edema Edetic Acid Endoscopes Endoscopy Enzyme-Linked Immunosorbent Assay Enzymes Erythema Glycerin Haptens Hemoglobin Immunoglobulins Intradermal Injection Ketamine Hydrochloride Lung Medetomidine Hydrochloride Mental Recall Mucus Pharmaceutical Preparations Proteins Saline Solution Skin Sterility, Reproductive Sulfate, Atropine Tissue, Adipose Tissues Trachea Tromethamine Ulcer Vaccination Vascular Access Ports Volumes, Packed Erythrocyte
The surgery was performed under the isoflurane inhalation anesthesia (Aerrane, Baxter HealthCare Corporation, Deerfield, IL, USA) using an animal anesthesia system (E-Z-7000 Classic System, E-Z-Anesthesia® Systems, Palmer, PA, USA). Additionally, a subcutaneous injection of 0.1–0.2 mL 0.5% bupivacaine into the surgery field and an intraperitoneal premedication with atropine sulfate 0.05 mg/kg in 1 mL 0.9% NaCl were performed. For the induction of anesthesia, 3.5–4% isoflurane mix with atmospheric air was used and for the maintenance of anesthesia the percentage of isoflurane was reduced to 2–2.5%. Transient 90 minutes middle cerebral artery occlusion (tMCAO) with MRI guiding was performed as described previously [57 (link)]. In brief, the bifurcation of the right common carotid artery was exposed. The monofilament with a rubber-coated tip (Doccol Corporation, Sharon, MA, USA, diameter 0.19 mm, length 30 mm; diameter with coating 0.37 ± 0.02 mm; coating length 3–4 mm) was inserted through the external carotid artery into the lumen of the internal carotid artery till the origin of the middle cerebral artery. The occlusion period lasted for 90 min, after which the monofilament was removed and the surgical wound was sutured, 3 mL of sterile saline was injected intraperitoneally and 30 mg/kg gentamicin sulfate was given intramuscularly. During recovery from anesthesia, the operated rats were placed in preheated cages. Rats with unsuccessful stroke modeling were excluded from the study after the MRI was performed 24 hours after operation (n = 8).
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Publication 2023
Anesthesia Anesthesia, Inhalation Animals Bupivacaine Cerebrovascular Accident Common Carotid Artery Dental Occlusion External Carotid Arteries Internal Carotid Arteries Isoflurane Middle Cerebral Artery Middle Cerebral Artery Occlusion Normal Saline Premedication Rattus Rubber Saline Solution Sterility, Reproductive Subcutaneous Injections Sulfate, Atropine Sulfate, Gentamicin Surgical Wound Transients

Top products related to «Sulfate, Atropine»

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Atropine sulfate is a chemical compound commonly used in laboratory settings. It acts as a muscarinic antagonist, blocking the action of the neurotransmitter acetylcholine on muscarinic receptors. The core function of atropine sulfate is to inhibit the parasympathetic nervous system, leading to physiological effects such as pupil dilation, reduced salivation, and altered heart rate.
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Atropine sulphate is a laboratory chemical compound used as a reference standard in analytical testing procedures. It is a salt of the alkaloid atropine and sulfuric acid. Atropine sulphate is commonly used to calibrate and validate analytical instruments and methods in various scientific applications.
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Atropine sulfate is a chemical compound commonly used in ophthalmic laboratory equipment. It is a white crystalline powder that acts as a parasympatholytic agent, primarily blocking the muscarinic receptors of the parasympathetic nervous system. This compound is often utilized in various ophthalmic applications, such as pupil dilation and the treatment of certain eye conditions.
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Xylazine is a pharmaceutical product used as a sedative and analgesic in veterinary medicine. It is a central alpha-2 adrenergic agonist that produces a calming effect and pain relief in animals. Xylazine is used to facilitate handling, examination, and minor surgical procedures in various animal species.
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Rompun is a veterinary drug used as a sedative and analgesic for animals. It contains the active ingredient xylazine hydrochloride. Rompun is designed to induce a state of sedation and pain relief in animals during medical procedures or transportation.
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Potassium chloride (KCl) is an inorganic compound that is commonly used as a laboratory reagent. It is a colorless, crystalline solid with a high melting point. KCl is a popular electrolyte and is used in various laboratory applications.
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Verapamil hydrochloride is a pharmaceutical ingredient manufactured by Merck Group. It is a white to off-white crystalline powder that is soluble in water. The primary function of verapamil hydrochloride is as a calcium channel blocker, which can be used in the development of various pharmaceutical products.
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Acetylcholine chloride is a chemical compound used in various laboratory applications. It is a salt that dissociates into acetylcholine and chloride ions in aqueous solutions. The primary function of acetylcholine chloride is to serve as a standard or reference material for the identification and quantification of acetylcholine in analytical procedures.
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Carbachol is a chemical compound that acts as an acetylcholine receptor agonist. It is commonly used in laboratory settings as a research tool to study the effects of acetylcholine on various biological systems.
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L-NAME is a synthetic compound that functions as a nitric oxide synthase inhibitor. It is commonly used in research applications to study the role of nitric oxide in biological processes.

More about "Sulfate, Atropine"

Sulfate is a crucial anion involved in various physiological processes, playing a vital role in biomedical research.
It is often studied alongside other important compounds like atropine, a tropane alkaloid with anticholinergic properties.
Atropine sulfate and atropine sulphate are common salt forms of atropine used in research and medical applications.
Researchers can optimize their work with these compounds using PubCompare.ai, an AI-driven platform that helps locate protocols from literature, preprints, and patents.
By employing AI-driven comparisons, PubCompare.ai identifies the best protocols and products, allowing researchers to improve their work and enhance their scientific findings.
In addition to sulfate and atropine, other related compounds like xylazine (also known as Rompun) and potassium chloride (KCl) are commonly used in biomedical research.
Verapamil hydrochloride, acetylcholine chloride, and carbachol are also relevant in studies involving the cardiovascular and nervous systems.
Researchers can leverage PubCompare.ai's cutting-edge tools to streamline their work with these compounds and improve the reproducibility and accuracy of their experiments.