The largest database of trusted experimental protocols

Tannins

Tannins are a diverse group of polyphenolic compounds found in various plants, including fruits, vegetables, and beverages.
These compounds have a wide range of biological activities, including antioxidant, anti-inflammatory, and antimicrobial properties.
Tannins are known to interact with proteins, carbohydrates, and other biomolecules, making them important in areas such as food science, medicine, and agriculture.
Research on tannins is crucial for understanding their potential applications and developing new products and therapies.
PubCompare.ai's AI-driven tools can help researchers optimize their tannins research by easily locating the best protocols from literature, pre-prints, and patents, and achieving reproducible science with data-driven insights.
One typo: Tannins are known to interact with proteins, carbohydraes, and other biomolecules.

Most cited protocols related to «Tannins»

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2004
Acetic Acid Anthocyanins Chalcones Coumaric Acids Coumarins Curcuminoid Ellagitannins Flavanones Flavones Flavonols formic acid Gallotannins High-Performance Liquid Chromatographies Hydroxybenzoic Acids Isoflavones Leucoanthocyanidins Lignans Medicinal Herbs Methanol Proanthocyanidins Quinones Retention (Psychology) sodium phosphate Stilbenes Tannins

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2015
4-phenylenediamine Allium cepa ARID1A protein, human Autopsy Blood Vessel Bruch Membrane Buffers Choroid Donors Edema Epoxy Resins Eye Glutaral Immersion Lens, Crystalline Light Lipids Lipofuscin Macula Lutea Melanosomes Microscopy Multimodal Imaging Optic Disk Osmium paraform Pathologic Neovascularization Phosphates Photoreceptor Cells Pigmentation Polybed 812 Radionuclide Imaging Retina Tannins Tissue, Membrane Tissues Tolonium Chloride Woman
A small portion of the dry extract was used for the phytochemical tests for compounds which include tannins, flavonoids, alkaloids, saponins, and steroids in accordance with the methods of [17 ,18 ] with little modifications. Exactly 1.0 g of plant extract was dissolved in10 ml of distilled water and filtered (using Whatman No 1 filter paper) A blue colouration resulting from the addition of ferric chloride reagent to the filtrate indicated the presence of tannins in the extract. Exactly 0.5 g of the plant extract was dissolved in 5 ml of 1% HCl on steam bath. A millilitre of the filtrate was treated with few drops of Dragendorff's reagent. Turbidity or precipitation was taken as indicative of the presence of alkaloid. About 0.2 g of the extract was dissolved in 2 ml of methanol and heated. A chip of magnesium metal was added to the mixture followed by the addition of a few drops of concentrated HCl. The occurrence of a red or orange colouration was indicative of the flavonoids. Freshly prepared 7% blood agar plate was used and wells were made in it. The crude extract dissolved in 10% methanol was used to fill the wells bored in the blood agar plates. Ten percent methanol was used as a negative control while commercial saponin solution was used as a positive control. The plates were incubated at 35°C for 6 h. complete haemolysis of the blood around the extract was indicative of saponin. About 0.5 g of the extract was dissolved in 3 ml of chloroform and filtered. Concentrated H2SO4 was carefully added to the filtrate to form lower layer. A reddish brown colour at the interface was taken as positive for steroid ring.
Full text: Click here
Publication 2010
Agar Alkaloids BLOOD Chloroform Complex Extracts DNA Chips ferric chloride Flavonoids Hemolysis Magnesium Metals Methanol Phytochemicals Plant Alkaloids Plant Extracts Saponin Saponins Steam Bath Steroids Tannins
Qualitative phytochemical analyses of both the extracts were performed by following the protocol of Adetuyi and Popoola [26 ], Trease and Evans [27 ], and Sofowora [28 ].
Tannins. 200 mg of plant material was boiled in 10 mL distilled water and few drops of FeCl3 were added to the filtrate; a blue-black precipitate indicated the presence of Tannins.
Alkaloids. 200 mg plant material was boiled in 10 mL methanol and filtered. 1% HCl was added followed by 6 drops of Dragendorff reagent, and brownish-red precipitate was taken as evidence for the presence of alkaloids.
Saponins (Frothing test). 5 mL distilled water was added to 200 mg plant material. 0.5 mL filtrate was diluted to 5 mL with distilled water and shaken vigorously for 2 minutes. Formation of stable foam indicates the presence of saponins.
Cardiac Glycosides (Keller-Kiliani test). 2 mL filtrate was treated with 1 mL glacial acetic acid containing few drops of FeCl3.Conc. H2SO4 was added to the above mixture giving green-blue colour depicting the positive results for presence of cardiac glycosides.
Steroids (Liebermann-Burchard reaction). 200 mg plant material was added in 10 mL chloroform. Acetic anhydride was added in the ratio of 1 : 1 which resulted into the formation of blue-green ring pointing towards the presence of steroids.
Terpenoids (Salkowski test). To 200 mg plant material 2 mL of chloroform (CHCl3) and 3 mL of concentrated sulphuric acid (H2SO4) were carefully added. A reddish brown colouration signified the presence of terpenoids.
Flavonoids. To the aqueous filtrate 5 mL of dilute ammonia solution was added, followed by concentrated H2SO4. A yellow colouration indicated the presence of flavonoids.
Phlobatannins. The deposition of a red precipitate denoted the presence of phlobatannins when 200 mg of plant material was dissolved in 10 mL of aqueous extract and few drops of 1% HCl were added in the boiling tube.
Anthraquinones. 500 mg of dried plant leaves were boiled in 10% HCl for 5 mins and filtrate was allowed to cool. Equal volume of CHCl3 with few drops of 10% NH3 was added to 2 mL filtrate. The formation of rose-pink colour implies the presence of Anthraquinones.
Reducing Sugars. To the 10 mL of aqueous extract a few drops of Fehling's solution A and B were added; an orange red precipitate suggests the presence of reducing sugars.
Full text: Click here
Publication 2014
Acetic Acid acetic anhydride Alkaloids Ammonia Anthraquinones Cardiac Glycosides Chloroform Flavonoids Methanol Phytochemicals Plant Leaves Plants Saponins Steroids Sugars Sulfuric Acids Tannins Terpenes
RBL-2H3 cells were allowed to settle overnight onto 15-mm round, clean glass coverslips in the presence of anti-DNP IgE (1 μg/ml) to prime cell surface FcεRI. After washing to remove excess IgE, FcεRI was cross-linked by incubation with DNP-BSA (0.1–1 μg/ml), with rabbit polyclonal anti-IgE (1 μg/ml) or with gold conjugates of these reagents (prepared as in Seagrave et al. 1991). Plasma membrane sheets were prepared by a modification of a procedure described by Sanan and Anderson 1991. The coverslips were rapidly chilled by immersion in ice-cold Hepes buffer (25 mM Hepes, pH 7, 25 mM KCl, and 2.5 mM MgAcetate) and inverted onto nickel EM grids that had been coated with formvar and carbon and, on the day of the experiment, glow-discharged and floated on poly-l-lysine (0.8 mg/ml for 30 min, followed by 10 s dH2O rinse and air drying). Pressure was applied to the coverslip for 20 s by bearing down with a cork. The coverslips were lifted, leaving sections of the upper cell surface adherent to the poly-l-lysine–coated grid. Membranes were rinsed in 4°C Hepes buffer and fixed in 2% paraformaldehyde for 10 min. For experiments using anti–IgE-gold to label FcεRI in the resting state, cells were fixed with 0.5% paraformaldehyde for 5 min and then incubated with anti–IgE-gold before inversion onto EM grids. All membranes were labeled from the inside by inverting the grids onto droplets containing primary antibodies or biotin-phalloidin (5 units/ml) followed by gold-conjugated secondary reagents. Incubations were for 30 min. Intermediate washes in PBS were performed by inverting the grids onto droplets. Primary antibodies were diluted in PBS, 0.1% BSA at the following concentrations: Syk, 10 μg/ml; FcεRI β, 28 μg/ml; Lyn, 2 μg/ml. Gold-conjugated secondary reagents were diluted 1:20 from commercial stocks in PBS-BSA. The samples were post-fixed in 2% glutaraldehyde in PBS and held overnight in PBS. Next, samples were stained for 10 min with 1% OsO4 prepared in 0.1 M cacodylate buffer and washed 5 min with cacodylate buffer and twice for 5 min in dH2O. Samples were then processed for 10 min in 1% aqueous tannic acid, followed by two 5-min rinses with dH2O, 10 min with 1% aqueous uranyl acetate and two 1-min rinses with dH2O. Grids were air-dried and examined using an Hitachi 600 transmission electron microscope.
Publication 2000
anti-IgE Antibodies ARID1A protein, human Biotin Buffers Cacodylate Carbon Cells Cold Temperature dinitrophenyl-bovine serum albumin Fc epsilon RI Formvar Glutaral Gold HEPES Inversion, Chromosome Lysine Nickel paraform Phalloidine Plasma Membrane Poly A Pressure Rabbits Submersion Tannins Tissue, Membrane Transmission Electron Microscopy uranyl acetate

Most recents protocols related to «Tannins»

Example 1

5 mg of oxidized CNT (MWCNT, average size: 12 nm×10 μm) are dispersed in 60 ml of Tris-HCl 10 mM in water (pH 8.5). The solution is ultra-sonicated until good dispersion is observed (about 1 minute). Dopamine hydrochloride (DA) is then added to reach a concentration of 0.1 mg/ml and the dispersion is stirred during 24 hours (h) at room temperature.

Example 6

Pda-coated CNT obtained according to the 2nd coating protocol were dispersed in a 50%/50% (by volume) mixture of water and ethanol so as to arrive at a CNT concentration of 0.5 mg/ml. The dispersion was sprayed on a glass substrate heated at 70° C. The number of sprayed layers was 50.

Example 13

The pda-coated CNT layer of example 6 was used to test electroless deposition. The sample was immersed in an electroless deposition solution during the desired time. The electroless solution contained glyoxilic acid (0.2 M) as reducing agent, EDTA (Ethylenediaminetetraacetic acid, 0.03 M) and CuSO4 (0.03 M). The solution was heated to between 50 and 60° C. and the pH was adjusted to 12-12.5 using NaOH. The immersion of the sample in the electroless solution led to delamination of the CNT layer from the glass substrate, probably due to H2 bubbles trapped between the CNT layer and the substrate. Although relatively fragile, the CNT layer conserved its cohesion and kept floating in the solution. When the CNT layer was progressively filled by copper, it turned became a more and more stable Cu-CNT composite (FIG. 13). This fabrication path is interesting as it allows fabricating self-supported Cu-CNT composites.

It may be worthwhile noting that delamination is not a necessary process step but it may be used to produce very thin CNT tissues. The ampacity of the composite of example 13 was slightly increased compared to copper foil in same conditions (about 8·104 A/cm2). Although this was not tested, it is expected that using CNT coated with pda containing copper seeds would improve the copper filling with the electroless deposition technique.

Example 14

125 ml of tannic acid (0.01 mg/ml)+CuSO4·5H2O (0.6 mg/ml) were prepared in water. 20 mg of oxidized CNT were added to 50 ml of this solution. The dispersion was periodically ultra-sonicated while adding tannic acid CuSO4 solution until a volume of 125 ml was reached. The dispersion was then periodically ultra-sonicated during 20 minutes. 75 ml of Tris-HCl solution (10 mM) was added and periodical ultra-sonications were carried out during 30 minutes. The pH was adjusted to a value ranging from 11 to 12 and the coated CNT were filtrated.

Example 15

The metal-ion-seeded coated CNT of example 14 were dispersed in 40 ml ethanol/water mixture (50%/50% by volume) so as to arrive at a concentration of 0.5 mg/ml. The dispersion was then sprayed in several layers on a on a Si—TaN (10 nm)-Ta (15 nm)-Cu (150 nm) substrate using the Paasche VL series airbrush (distance from the substrate about 15 cm. The substrate temperature was 90° C. The resulting sprayed layer (FIG. 14) had a thickness of about 3.5 μm.

Example 16

The CNT layer of example 15 was subjected to electroplating in an aqueous 0.1 M CuSO4 solution (at room temperature). The pH was adjusted to 1 by addition of H2SO4. During the electroplating (potential: −0.2 V vs SCE, duration: 30 minutes) the solution was stirred. The resulting composite (FIGS. 15 and 16) had a thickness of about 5 μm.

While specific embodiments have been described herein in detail, those skilled in the art will appreciate that various modifications and alternatives to those details could be developed in light of the overall teachings of the disclosure. Accordingly, the particular arrangements disclosed are meant to be illustrative only and not limiting as to the scope of the invention, which is to be given the full breadth of the appended claims and any and all equivalents thereof.

Full text: Click here
Patent 2024
Acids Copper Edetic Acid Ethanol Figs Hydrochloride, Dopamine Light Metals Reducing Agents Submersion Tannins Teaching Tissues Tromethamine
The Folin–Ciocalteu method described by Govindappa et al. [36 ] was used to determine the total tannin content in Vernonia guineensis extract. The mixture consisting of extract (100 µl, 2 mg/ml), Folin–Ciocalteu reagent (500 µl, diluted 10 times in water), sodium carbonate (1000 µl, 35%), and distilled water (8.4 ml) was shaken, incubated (room temperature, 30 minutes), and the absorbance (700 nm, spectrophotometer) was read. The various concentrations of tannic acid (100 to 500 µg/ml) were used to draw the standard curve which made it possible to calculate the content of total tannins in the extract.
Full text: Click here
Publication 2023
folin sodium carbonate Tannins Vernonia
The 6-well plates containing Ctr-infected MSCs treated and untreated with DFMO (20 mM) were taken at time intervals of 24 h, 48 h and 72 h, washed twice with cold PBS and fixed with 2.5% glutaraldehyde. Fixed cells were detached by a rubber policeman. The cells were post-fixed with 1% osmium tetroxide (OsO4) and then contrasted with tannic acid and uranyl acetate. The specimens were dehydrated in a graded ethanol series (50–100%) and embedded in agar 100. Ultrathin sections (70 nm) were produced, contrasted with lead citrate and examined with a Zeiss EM 10 scanning electron microscope and LEO 912AB transmission electron microscope (Carl Zeiss AG, Oberkochen, Germany).
Full text: Click here
Publication 2023
Agar Cells Citrates Common Cold Eflornithine Ethanol Glutaral Osmium Tetroxide Rubber Scanning Electron Microscopy Tannins Transmission Electron Microscopy uranyl acetate
Food grade cellulase (within a pH range of 3.0 to 6.5 and a temperature range of 35 to 75 °C), xylanase (within a pH range of 4.0 to 9.0 and a temperature range of 25 to 75 °C), and pectinase (within a pH range of 3 to 6.5 and a temperature range of 35 to 75 °C) were purchased from Winovazyme (Beijing, China). Porcine pancreatic α-amylase (with an optimal temperature of 20 °C and an optimal pH of 7.4), porcine pancreatic lipase (with an optimal temperature of 37 °C and an optimal pH of 7), and lysozyme were purchased from Sigma Aldrich (St. Louis, MO, USA). Tannic acid, methyl gallate, gallic acid, rhodanine, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), potassium sulfate, quercetin, and Folin-Ciocalteu’s phenol reagent were all of analytical grade and of the highest quality available from Sigma-Aldrich. High-performance liquid chromatography (HPLC) grade standard (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG), (-)-epigallocatechin (EGC), (-)-epicatechin (EC), (-)-gallocatechin gallate (GCG), (-)-catechin gallate (CG), (-)-gallocatechin (GC), (+)-catechin (C), caffeine, and gallic acid (GA) were all purchased from Sigma. All chemicals used for antioxidant activity assays and enzyme production were of analytical grade and were obtained from RCI Labscan (Bangkok, Thailand). The medium ingredients used in this study, such as agar, yeast extract, and malt extract, were all purchased from HiMedia (Nashik, India).
Full text: Click here
Publication 2023
Agar alpha-Amylases Antioxidant Activity Biological Assay Caffeine Carboxylic Acids Catechin catechin gallate Cellulase diphenyl Enzymes Epicatechin epicatechin-3-gallate epigallocatechin epigallocatechin gallate folin Food Gallic Acid gallocatechin gallate gallocatechol High-Performance Liquid Chromatographies Lipase methyl gallate Muramidase Pancreas Phenol Pigs Polygalacturonase potassium sulfate Quercetin Rhodanine Tannins Trolox C Yeast, Dried
A single colony of Sporidiobolus ruineniae A45.2 was inoculated in yeast extract-malt extract broth (YMB) (3 g/L yeast extract, 3 g/L malt extract, 10 g/L glucose) and incubated at 30 °C on a 150-rpm rotary shaker for 24 h. Accordingly, 10% (v/v) of inoculum was transferred to YMB supplemented with 1% (w/v) filtered sterile tannic acid and incubated at the same conditions as have been described above. After 48 h of cultivation, the culture was harvested by centrifugation. Cell pellets were washed with 20 mM sodium phosphate buffer pH 6.5 supplemented with 0.1% (v/v) Triton X-100 to remove any gallic acid attached to the yeast cell wall and the residual tannic acid. The cell pellets were then suspended with 20% (w/v) sucrose in 30 mM Tris-HCl pH 8.0. To release the tannase associated with the cell envelope [19] (link), the suspension was supplemented with lysozyme solution prepared in 100 mM EDTA pH 7.3 to yield a final concentration of 0.1 mg/mL of lysozyme prior to being incubated on ice for 40 min. The lysozyme-EDTA treated suspension was centrifuged at 17,350 × g for 15 min at 4 °C. The supernatant was then dialyzed against 20 mM sodium phosphate buffer pH 7.0 at 4 °C until equilibrium was reached. The resulting dialyzed enzyme was then used in further experiments.
Tannase activity was determined according to the method described in a previous study [20] (link) with slight modifications. Briefly, 50 μL of the enzyme solution was mixed with 50 μL of the substrate (12.5 mM methyl gallate in 100 mM sodium phosphate buffer pH 6.5). The reaction was carried out at 37 °C for 20 min. Then, 60 μL of 0.667% (w/v) methanolic rhodanine solution was added into the reaction mixture to stop the reaction and to detect the release of gallic acid from tannic acid. After a 5 min period of incubation at room temperature (25 °C), a pinkish purple color was visualized by adding 40 μL of 0.5 M KOH and the mixture was left at room temperature for 5 min. Finally, 800 μL of distilled water was added, the mixture was vigorously mixed, and absorbance was measured at 520 nm. One unit of tannase was defined as the amount of enzyme that released 1 μmol of gallic acid in 1 min under the assay conditions.
Full text: Click here
Publication 2023
Biological Assay Buffers Cells Cell Wall Centrifugation Edetic Acid Enzymes Gallic Acid Glucose Methanol methyl gallate Muramidase Pellets, Drug Rhodanine Rhodosporidiobolus ruineniae sodium phosphate Sterility, Reproductive Sucrose tannase Tannins Triton X-100 Tromethamine Yeast, Dried

Top products related to «Tannins»

Sourced in United States, Germany, India, Italy, China, Brazil, United Kingdom, France, Poland, Spain, Australia, Canada, Denmark, Sao Tome and Principe, Czechia
Tannic acid is a naturally occurring organic compound found in various plant sources. It serves as a key component in numerous laboratory applications, functioning as a reagent, precipitating agent, and astringent. Tannic acid exhibits a high degree of solubility in water and other polar solvents.
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
Sourced in United States
The AMT Imaging System is a high-performance imaging solution designed for advanced microscopy applications. It features a modular architecture and supports a range of imaging techniques, including brightfield, darkfield, and fluorescence microscopy. The system provides exceptional image quality and resolution, enabling detailed analysis and observation of a wide variety of samples.
Sourced in Japan, United States, United Kingdom, Germany, Spain
The JEM 1010 is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-quality imaging and analysis of sample materials at the nanoscale level. The JEM 1010 utilizes an electron beam to illuminate and interact with the sample, allowing for the observation and study of microscopic structures and features.
Sourced in United States, Germany, Italy, India, France, Poland, Spain, China, Chile, Sao Tome and Principe, United Kingdom, Switzerland, Australia, Brazil, Canada, Singapore, Portugal, Mexico, Malaysia, New Zealand, Macao, Croatia, Belgium, Lithuania, Romania, Argentina, Finland
The Folin-Ciocalteu reagent is a colorimetric reagent used for the quantitative determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that undergo a color change when reduced by phenolic compounds.
Sourced in United States, Germany, Italy, India, China, Spain, Poland, France, United Kingdom, Australia, Brazil, Singapore, Switzerland, Hungary, Mexico, Japan, Denmark, Sao Tome and Principe, Chile, Malaysia, Argentina, Belgium, Cameroon, Canada, Ireland, Portugal, Israel, Romania, Czechia, Macao, Indonesia
DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.
Sourced in United States, Germany, United Kingdom, France, Italy, India, China, Sao Tome and Principe, Canada, Spain, Macao, Australia, Japan, Portugal, Hungary, Brazil, Singapore, Switzerland, Poland, Belgium, Ireland, Austria, Mexico, Israel, Sweden, Indonesia, Chile, Saudi Arabia, New Zealand, Gabon, Czechia, Malaysia
Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
Sourced in United States, Germany, Italy, France, Australia, India, Spain, United Kingdom, China, Poland, Sao Tome and Principe, Japan, Portugal, Canada, Switzerland, Brazil, Malaysia, Singapore, Macao, Belgium, Ireland, Mexico, Hungary
Catechin is a natural polyphenolic compound found in various plants, including green tea. It functions as an antioxidant, with the ability to scavenge free radicals and protect cells from oxidative stress.
Sourced in United States, Germany, Italy, India, France, Spain, United Kingdom, Australia, Switzerland, Poland, Portugal, China, Canada, Sao Tome and Principe, Brazil, Ireland, Mexico, Sweden, Hungary, Singapore, Malaysia, Pakistan, Thailand, Cameroon, Japan, Chile
Sodium carbonate is a water-soluble inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a pH regulator, water softener, and cleaning agent in various industrial and laboratory applications.

More about "Tannins"

Tannic acid, Gallic acid, Quercetin, AMT Imaging System, JEM 1010 transmission electron microscope, Folin-Ciocalteu reagent, DPPH, Ascorbic acid, Catechin, Sodium carbonate