Testosterone
It is produced primarily by the testes in men and the ovaries and adrenal glands in women.
Testosterone is essential for the regulation of various physiological processes, including sexual function, muscle growth, bone density, and red blood cell production.
Researchers studying testosterone may utilize PubCompare.ai, an AI-driven platform, to optimize their research by locating the best protocols from literature, pre-prints, and patents using intelligent comparisons.
This tool can enhance reproducibility and accuracy by providing powerful analysis tools, allowing researchers to experience the future of testosterone research today.
Most cited protocols related to «Testosterone»
Castration of adult male mice was performed using standard techniques. For tamoxifen induction of Cre activity in mice containing Nkx3.1CreERT2/+, mice were administered 9 mg/40 g tamoxifen for 4 consecutive days. For prostate regeneration, physiological levels of testosterone (1.875 µg/hr) were administered for four weeks by subcutaneous implantation of mini-osmotic pumps (Alzet)45 (link). When included, BrdU (100 mg/kg) was administered once daily during the first three days of regeneration. For single-cell transplantation, single YFP+ cells were isolated by mouth-pipetting under epifluorescence illumination from a dissociated prostate cell suspension obtained from castrated and tamoxifen-induced Nkx3.1CreERT2/+; R26R-YFP/+ mice. A single YFP+ cell (or YFP− cell as a control) was recombined with 2.5 × 105 rat urogenital sinus mesenchyme cells in a 10 µl collagen pad, followed by transplantation under the kidney capsule of nude mice and harvesting after 10–12 weeks.
Cryosections were stained with primary antibodies as listed in
Most recents protocols related to «Testosterone»
EXAMPLE 1
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- 1. Dutasteride was dissolved in ethanol
- 2. Medium chain triglycerides and castor oil was added to contents of step 1 to form the solution.
- 3. The above solution was filled into suitable containers.
The hair growth and hair thickness measurement of was conducted in Wistar rats. Wistar rats was divided into groups, each group having 13 animals. The study on the Wistar rats was conducted for 21 days. On day “0” of the study, fur over and around the flank organs of Wistar rats was shaved with electric clippers and the area of 2×2 cm was used for topical application of dutasteride compositions of examples 2 to 13 at a dose of 100 μl/kg of example 2 to Example 13 along with the compositions of reference example 1 (Finasteride oral at a dose of 0.1 mg/kg) for a period of 21 days once daily (every day between 10 and 11 pm). 100 μl of 1% testosterone was injected subcutaneously daily for 21 days (at 9 am every day) and effect (hair growth and thickness) was evaluated on 22nd day after sacrificing the animals. The normal control of shaved rats (without the administration of testosterone) was placed with a group consisting of 13 animals.
The change in hair growth was measured by visual scoring (hair growth score) on the 13 animals of each group and the mean was calculated. The visual scoring was calculated based on following parameters
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- Score 0: no hair growth observed
- Score 1: less than 20% growth observed
- Score 2: 20% to less than 40% growth observed
- Score 3: 40% to less than 60% growth observed
- Score 4: 60% to less than 80% growth observed
- Score 5: 80% to 100% growth
The visual scoring of mean of 13 animals in each group treated with compositions of example 2 to 13 along with reference oral finasteride and normal control was depicted in Table 7.
The hair thickness was measured by Caslite hair analysing instrument attached to microscope at 200× magnification and results of hair thickness (μm) in each group treated with compositions of example 2 to 13 along with reference oral finasteride and normal control was depicted in Table 7.
The data in the Table 7 shows the hair growth score and hair thickness increased significantly in the Wistar rats of example 2 to 4, most preferably the composition of example 3 consisting of Dutasteride 0.022 wt % (equivalent to 0.02% w/v), Castor Oil 40 wt %, Medium chain triglycerides 30 wt % and Ethanol: qs to 100 wt % (about 30 wt %) has highest hair growth score and hair thickness when compared to the other formulations. The rapid onset of the effects of the above described composition for topical application of the present invention can significantly improve the treatment compliance. That is, in case of the conventional preparations (finasteride oral) the onset of effects is similar to that of the formulation as disclosed in example 3 at the reduced dosage with topical administration and has little side-effects, when compared to the oral finasteride.
Example 1
In comparative example 1, Finasteride active ingredient was dissolved to prepare compositions comprising the oral medication using the following ingredients as shown in Table 6.
Oral Finasteride medication administered in rats at 0.1 mg/kg corresponds to 1 mg human dose.
Example 1
CYP-enzyme phenotyping using human liver microsomes and recombinant CYP enzymes revealed that mitapivat sulfate was primarily metabolized by CYP3A4/5 (>90%), with minor contributions from CYP2C9, CYP2C8, and CYP1A2. There was evidence of metabolism-dependent inhibition of CYP2C19 (largely reversible) and CYP3A4 (largely irreversible). Mitapivat sulfate was found to be a substrate and inhibitor for P-gp but not for breast cancer resistance protein (BCRP) and was found to be a potential inducer of human CYP2B6 and CYP3A4. Mitapivat sulfate appeared to be a mild inhibitor of CYP2C8, CYP2C9, xYP2C19, CYP2D6, and CYP3A4/5 enzymes (testosterone 6β-hydroxylation), bile salts export pump (BSEP), organic anion transporting polypeptide (OATP)1B1, organic anion transporter (OAT)3, and organic cation transporter (OCT)2, and of uridine-5′-diphospho-glucuronosyltransferase (UGT) 1A3, 1A4, and 1A9. Mitapivat sulfate does not appear to be an inhibitor of multidrug resistance-associated protein (MRP)2, MRP3, OATP1B3, and OAT1. The metabolite was found to be a mild inhibitor of CYP2C9 and CYP2C19 and was not an inhibitor of P-gp or BCRP under tested concentrations.
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More about "Testosterone"
Produced primarily by the testes in men and the ovaries and adrenal glands in women, this essential compound regulates a wide range of physiological processes, including sexual function, muscle growth, bone density, and red blood cell production.
Researchers exploring the intricacies of testosterone may leverage the power of PubCompare.ai, an AI-driven platform that enables the optimization of their research efforts.
This innovative tool allows scientists to locate the best protocols from a vast array of literature, pre-prints, and patents, utilizing intelligent comparison techniques.
By harnessing the capabilities of PubCompare.ai, researchers can enhance the reproducibility and accuracy of their testosterone studies, empowering them to experience the future of testosterone research today.
The platform's powerful analysis tools provide invaluable insights, facilitating informed decision-making and driving advancements in the field.
Beyond testosterone, researchers may also explore related concepts such as Immulite 2000, a widely used automated immunoassay system; Progesterone, another crucial steroid hormone; FBS (Fetal Bovine Serum), a commonly used cell culture supplement; Phenacetin, a historical analgesic drug; Cobas e411, a popular automated immunoassay analyzer; 6β-hydroxytestosterone, a key metabolite of testosterone; Chlorzoxazone, a muscle relaxant; and Corticosterone, a glucocorticoid hormone.
By understanding these interconnected topics, researchers can gain a more comprehensive understanding of the complex physiological landscape surrounding testosterone.
Experence the future of testosterone research today with PubCompare.ai, and unlock the full potential of your research endeavors.