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Testosterone

Testosterone is a steroid hormone that plays a crucial role in the development and maintenance of male sexual characteristics.
It is produced primarily by the testes in men and the ovaries and adrenal glands in women.
Testosterone is essential for the regulation of various physiological processes, including sexual function, muscle growth, bone density, and red blood cell production.
Researchers studying testosterone may utilize PubCompare.ai, an AI-driven platform, to optimize their research by locating the best protocols from literature, pre-prints, and patents using intelligent comparisons.
This tool can enhance reproducibility and accuracy by providing powerful analysis tools, allowing researchers to experience the future of testosterone research today.

Most cited protocols related to «Testosterone»

Wild type adult male and female zebrafish, Danio rerio, were obtained from a commercial supplier (Ekkwill, Gibsonton, FL) and maintained in 30 gal aquaria at 28°C on a 14:10 light-dark cycle. Fertilized eggs were collected after natural spawning, washed, and distributed into 20 × 100 mm culture plates (Fisher Scientific). Embryos (150 embryos/50 ml egg water) were allowed to develop at 28°C on a 14L:10D cycle [36 ]. For developmental expression analysis embryos were collected after timed intervals: 2, 6, 12, 24, 48, 72, and 120 hours post-fertilization (hpf), quick-frozen on dry ice, and stored at -70°C until analysis (3 independent embryo pools, 50 embryos per pool, per time point from the same spawning group). For treatment expression analysis embryos were left untreated until 24 hpf and then exposed to 17β-estradiol (E2; 0.1 μM), testosterone (T; 1 μM), ICI 182,780 (ICI; 10 μM; Tocris Bioscience, Ellisville, MO), β-napthaflavone (BNF; 10 nM), or 2,3,7,8, tetrachlodibenzo-p-dioxin (TCDD; 1 nM; Ultra Scientific, N. Kingstown, RI) dissolved in dimethyl sulfoxide (DMSO). All chemicals were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Stock solutions of chemicals were added directly to egg water and replaced daily. In addition, embryos were treated with DMSO alone (final concentration, 0.0006%), EtOH alone (final concentration 0.0005%), or left untreated as a control. Embryos were collected at 96 hpf, quick-frozen on dry ice, and stored at -70°C until analysis (3 independent embryo pools per treatment). Treated embryo RNAs were used for both housekeeping gene expression analysis (Table 3) and gene of interest normalization (Figure 2). Tissues (brain, eye, heart, liver, muscle, gonad) were collected from adult male and female zebrafish, pooled by sex (3 pools per tissue type/sex, 5 fish per pool), quick-frozen on dry ice, and stored at -70°C. Adult fish were reproductively active stock from our breeding colony.
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Publication 2008
Adult Brain Dioxins Dry Ice Embryo Embryonic Development Estradiol Ethanol Females Fertilization Fishes Freezing Gene Expression Profiling Genes Gonads Heart Histocompatibility Testing ICI 182780 Liver Males Muscle Tissue RNA Sulfoxide, Dimethyl Testosterone Tetrachlorodibenzodioxin Tissues Zebrafish Zygote
The Nkx3.1CreERT2/+ allele was generated by gene targeting using standard techniques; the Nkx3.1 null mutant mice have been previously described21 (link). R26R-lacZ and Pten conditional mutant mice were obtained from the Jackson Laboratory Induced Mutant Resource; the R26R-YFP mice were provided by Dr. Frank Costantini. All lines were maintained on a hybrid C57BL/6-129/Sv strain background.
Castration of adult male mice was performed using standard techniques. For tamoxifen induction of Cre activity in mice containing Nkx3.1CreERT2/+, mice were administered 9 mg/40 g tamoxifen for 4 consecutive days. For prostate regeneration, physiological levels of testosterone (1.875 µg/hr) were administered for four weeks by subcutaneous implantation of mini-osmotic pumps (Alzet)45 (link). When included, BrdU (100 mg/kg) was administered once daily during the first three days of regeneration. For single-cell transplantation, single YFP+ cells were isolated by mouth-pipetting under epifluorescence illumination from a dissociated prostate cell suspension obtained from castrated and tamoxifen-induced Nkx3.1CreERT2/+; R26R-YFP/+ mice. A single YFP+ cell (or YFP cell as a control) was recombined with 2.5 × 105 rat urogenital sinus mesenchyme cells in a 10 µl collagen pad, followed by transplantation under the kidney capsule of nude mice and harvesting after 10–12 weeks.
Cryosections were stained with primary antibodies as listed in Supp. Table 5, and counterstained with TOPRO3 or DAPI (Invitrogen/Molecular Probes). Secondary antibodies were labeled with Alexa Fluor 488 , 555, or 594 (Invitrogen/Molecular Probes). Immunofluorescence staining was imaged using a Leica TCS5 spectral confocal microscope. Cell counting was performed manually using confocal photomicrographs with at least three animals for each experiment or genotype analyzed.
Publication 2009
Adult alexa fluor 488 Alleles Animals Antibodies Bromodeoxyuridine Capsule Cells Cell Transplantation Collagen Cryoultramicrotomy DAPI Fluorescent Antibody Technique Genotype Hybrids Kidney LacZ Genes Light Male Castration Mesenchyma Mice, Knockout Mice, Nude Microscopy, Confocal Molecular Probes Mus Oral Cavity Orchiectomy Osmosis Ovum Implantation Photomicrography physiology Prostate PTEN protein, human Regeneration Sinuses, Nasal Strains System, Genitourinary Tamoxifen Testosterone Transplantation
The demonstration cases described below feature published microarray gene-expression data. They were converted to ranked lists by calculating t-test P-values between the relevant sample types (classes) for each probe, that were log10-transformed and signed to indicate direction of change (positive for increased in Class B, negative for increased in Class A or decreased in Class B). Probes were annotated with current mouse or human UniGene identifiers and homologs were identified using HomoloGene; only the probe with the highest absolute signed t-test P-value within those with matching UniGene identifiers was kept in the collapsing step. Data downloaded from Gene-expression Omnibus (GEO) (19 (link)): MPAKT prostate cancer mouse model, GSE1413; breast tumors with ER, PR and HER2 status, GSE2603; MMTV-HER2/neu mouse model, GSE2528; BCR-ABL transfected cell line, GSE10912; mammary stem cell, GSE3711; KRAS2 overexpression cell line, GSE3151; lung tumors with KRAS2 status, GSE3141; imatinib treatment in leukemia patients, GSE2535; dasatinib treatment in cell lines, GSE9633 and GSE6569; castration and testosterone treatment in mice, GSE5901; gedunin treatment in prostate cancer cell line, GSE5506. Data downloaded from Array Express (20 (link)): imatinib treatment in leukemia patients, E-MEXP-433 (21 (link)). Data downloaded from an individual lab website: KRAS2 lung tumors and mouse model (1 (link)), Cmap database (10 (link)). Data communicated by collaborators: PTEN knockout prostate cancer mouse model, Dr Shunyou Wang, laboratory of Dr Hong Wu, UCLA (22 (link)).
Publication 2010
Breast Breast Neoplasm Castration Cell Lines Dasatinib erbb2 Gene ERBB2 protein, human gedunin Gene Expression Homo sapiens Imatinib KRAS protein, human Leukemia Lung Neoplasms Mice, Knockout Microarray Analysis Mouse mammary tumor virus Mus Patients Prostate Cancer PTEN protein, human Stem Cells Testosterone
Tolbutamide was purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). 4-hydroxyTolbutamide and 6-hydroxychlorzoxazone were obtained from Toronto Research Chemicals Inc. (North York, Canada). Dextromethorphan, dextrorphan and chlorzoxazone were supplied by Sigma-Aldrich Co. (St Louis, MO, USA). Testosterone was obtained from International Laboratory Limited (San Bruno, CA, USA). 6β-hydroxytestosterone was purchased from BD Biosciences Co. (Woburn, MA, USA). Phenacetin, cortisone acetate, EB and EE were from National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). NADPH was obtained from Roche Diagnostics GmbH (Mannheim, Germany). All other reagents were of HPLC or analytical grade.
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Publication 2014
4'-hydroxytolbutamide 6-hydroxychlorzoxazone Biological Products Chlorzoxazone Cortisone Acetate Dextromethorphan Dextrorphan Diagnosis High-Performance Liquid Chromatographies NADP Pharmaceutical Preparations Phenacetin Testosterone Tolbutamide
Bioavailable 25-hydroxyvitamin D was defined as circulating 25-hydroxyvitamin D not bound to vitamin D–binding protein, which is analogous to the definition of bioavailable testosterone.28 (link) Concentrations of bioavailable 25-hydroxyvitamin D were calculated in 1025 homozygotes, for whom we could use a single genotype-specific binding affinity constant on the basis of the presence of a single vitamin D–binding protein variant (see the Methods section in the Supplementary Appendix).22 (link) Calculated concentrations of bioavailable 25-hydroxyvitamin D were validated by direct measurement in a subgroup of homozygous participants with the use of a competitive radioligand-binding assay (see the Methods section and Fig. S2 and S3 in the Supplementary Appendix). Measured and calculated 25-hydroxyvitamin D concentrations were correlated (Pearson's r = 0.81 in 32 Gc1F homozygotes and 0.90 in 13 Gc1S homozygotes; P<0.001 for both relationships) (Fig. S4 in the Supplementary Appendix).
Publication 2013
25-hydroxyvitamin D Ergocalciferol Genotype Homozygote Mutant Proteins Radioligand Assay Testosterone Vitamin D-Binding Protein

Most recents protocols related to «Testosterone»

EXAMPLE 1

TABLE 1
IngredientPercent (w/w)
Dutasteride0.005-1%
Castor Oil  30-50%
Medium Chain Triglycerides  25-35%
Ethanol  25-35%
Process for Preparation
    • 1. Dutasteride was dissolved in ethanol
    • 2. Medium chain triglycerides and castor oil was added to contents of step 1 to form the solution.
    • 3. The above solution was filled into suitable containers.

The hair growth and hair thickness measurement of was conducted in Wistar rats. Wistar rats was divided into groups, each group having 13 animals. The study on the Wistar rats was conducted for 21 days. On day “0” of the study, fur over and around the flank organs of Wistar rats was shaved with electric clippers and the area of 2×2 cm was used for topical application of dutasteride compositions of examples 2 to 13 at a dose of 100 μl/kg of example 2 to Example 13 along with the compositions of reference example 1 (Finasteride oral at a dose of 0.1 mg/kg) for a period of 21 days once daily (every day between 10 and 11 pm). 100 μl of 1% testosterone was injected subcutaneously daily for 21 days (at 9 am every day) and effect (hair growth and thickness) was evaluated on 22nd day after sacrificing the animals. The normal control of shaved rats (without the administration of testosterone) was placed with a group consisting of 13 animals.

The change in hair growth was measured by visual scoring (hair growth score) on the 13 animals of each group and the mean was calculated. The visual scoring was calculated based on following parameters

    • Score 0: no hair growth observed
    • Score 1: less than 20% growth observed
    • Score 2: 20% to less than 40% growth observed
    • Score 3: 40% to less than 60% growth observed
    • Score 4: 60% to less than 80% growth observed
    • Score 5: 80% to 100% growth

The visual scoring of mean of 13 animals in each group treated with compositions of example 2 to 13 along with reference oral finasteride and normal control was depicted in Table 7.

The hair thickness was measured by Caslite hair analysing instrument attached to microscope at 200× magnification and results of hair thickness (μm) in each group treated with compositions of example 2 to 13 along with reference oral finasteride and normal control was depicted in Table 7.

TABLE 7
Hair growthHair thickness
Example NoCompositionscore(μm)
2Dutasteride 0.011 wt %4.1562.08
(0.01% w/v)
Castor Oil 40 wt %
Medium chain triglycerides
30 wt %
Ethanol-qs to 100 wt %
3Dutasteride 0.022 wt %4.8567.92
(0.02% w/v)
Castor Oil 40 wt %
Medium chain triglycerides
30 wt %
Ethanol-qs to 100 wt %
4Dutasteride 0.056 wt %4.6958
(0.05% w/v)
Castor Oil 40 wt %
Medium chain triglycerides
30 wt %
Ethanol-qs to 100 wt %
5Dutasteride 0.012 wt %4.0856.92
(0.01% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
6Dutasteride 0.024 wt %3.6960.08
(0.02% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
7Dutasteride 0.061 wt %4.1561.54
(0.05% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
8Dutasteride 0.011 wt %3.3843.15
(0.01% w/v)
Castor Oil 75 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
9Dutasteride 0.021 wt %3.8559
(0.02% w/v)
Castor Oil 75 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
10Dutasteride 0.054 wt %3.5450.85
(0.05% w/v)
Castor Oil 75 wt %
Medium chain triglycerides
12.5 wt %
Ethanol-qs to 100 wt %
11Dutasteride 0.011 wt %3.3149.23
(0.01% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
75 wt %
Ethanol-qs to 100 wt %
12Dutasteride 0.022 wt %4.0855.23
(0.02% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
75 wt %
Ethanol-qs to 100 wt %
13Dutasteride 0.054 wt %3.6945.69
(0.05% w/v)
Castor Oil 12.5 wt %
Medium chain triglycerides
75 wt %
Ethanol-qs to 100 wt %
ReferenceFinasteride oral4.8564.75
Example 1
Normal ControlNormal Control4.4666

The data in the Table 7 shows the hair growth score and hair thickness increased significantly in the Wistar rats of example 2 to 4, most preferably the composition of example 3 consisting of Dutasteride 0.022 wt % (equivalent to 0.02% w/v), Castor Oil 40 wt %, Medium chain triglycerides 30 wt % and Ethanol: qs to 100 wt % (about 30 wt %) has highest hair growth score and hair thickness when compared to the other formulations. The rapid onset of the effects of the above described composition for topical application of the present invention can significantly improve the treatment compliance. That is, in case of the conventional preparations (finasteride oral) the onset of effects is similar to that of the formulation as disclosed in example 3 at the reduced dosage with topical administration and has little side-effects, when compared to the oral finasteride.

Example 1

In comparative example 1, Finasteride active ingredient was dissolved to prepare compositions comprising the oral medication using the following ingredients as shown in Table 6.

TABLE 6
Comparative
IngredientExample 1
Finasteride0.1 mg
Polyethylene Glycol 4000.060ml
Purified waterQs to 1 ml

Oral Finasteride medication administered in rats at 0.1 mg/kg corresponds to 1 mg human dose.

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Patent 2024
Animals Castor oil Dutasteride Electricity Ethanol Finasteride Hair Homo sapiens Microscopy Pharmaceutical Preparations polyethylene glycol 400 Rats, Wistar Rattus norvegicus Testosterone Triglycerides

Example 1

CYP-enzyme phenotyping using human liver microsomes and recombinant CYP enzymes revealed that mitapivat sulfate was primarily metabolized by CYP3A4/5 (>90%), with minor contributions from CYP2C9, CYP2C8, and CYP1A2. There was evidence of metabolism-dependent inhibition of CYP2C19 (largely reversible) and CYP3A4 (largely irreversible). Mitapivat sulfate was found to be a substrate and inhibitor for P-gp but not for breast cancer resistance protein (BCRP) and was found to be a potential inducer of human CYP2B6 and CYP3A4. Mitapivat sulfate appeared to be a mild inhibitor of CYP2C8, CYP2C9, xYP2C19, CYP2D6, and CYP3A4/5 enzymes (testosterone 6β-hydroxylation), bile salts export pump (BSEP), organic anion transporting polypeptide (OATP)1B1, organic anion transporter (OAT)3, and organic cation transporter (OCT)2, and of uridine-5′-diphospho-glucuronosyltransferase (UGT) 1A3, 1A4, and 1A9. Mitapivat sulfate does not appear to be an inhibitor of multidrug resistance-associated protein (MRP)2, MRP3, OATP1B3, and OAT1. The metabolite was found to be a mild inhibitor of CYP2C9 and CYP2C19 and was not an inhibitor of P-gp or BCRP under tested concentrations.

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Patent 2024
ABCB11 protein, human ABCG2 protein, human CYP2C19 protein, human Cytochrome P-450 CYP1A2 Cytochrome P-450 CYP2C8 Cytochrome P-450 CYP2D6 Cytochrome P-450 CYP3A4 Enzymes Glucuronosyltransferase Homo sapiens Hydroxylation Metabolism Microsomes, Liver mitapivat sulfate Multidrug-Resistance Associated Protein 2 Organic Anion Transporters Organic Anion Transport Polypeptides Psychological Inhibition SLC22A2 protein, human Testosterone Uridine
Serum testosterone levels were measured weekly from 1 week after the first Diphereline injection. Blood samples were collected from the orbital sinus using capillary tubes after anesthesia at the same time every week, and only serum was isolated by centrifugation at 3000 rpm for 10 min at 4°C. Testosterone levels were measured by Seoul Clinical Laboratories (SCL, Yongin, South Korea) using an electrochemiluminescence assay (ECLIA, Roche Diagnostics, Indianapolis, IN, USA).
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Publication 2023
Anesthesia Biological Assay BLOOD Capillaries Centrifugation Clinical Laboratory Services Diagnosis Serum Sinuses, Nasal Testosterone

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Publication 2023
Albumins alpha-Amylases Antibodies Aspartate Transaminase BLOOD Blood Coagulation Factor C-Peptide Care, Prenatal Cholesterol Complete Blood Count Creatinine Electrolytes Ferritin gamma-Glutamyl Transpeptidase Glucose Human Chorionic Gonadotropin Hydrocortisone IL6 protein, human Insulin Iron Mothers Nonesterified Fatty Acids Plasma Testosterone Transaminases Triglycerides Urea Uric Acid
Incubations were performed with 0.1 mg/mL human liver microsomes (purchased from BD Gentest as a pooled batch from 150 donors) in a final incubation volume of 0.25 mL. The incubation medium contained 0.05 M phosphate buffer (pH 7.4) containing an NADPH regenerating system (including 1.3 mM NADP, 3.3 mM glucose 6-phosphate,3.3 mM MgCl2, and 1.0 U/mL glucose 6-phosphate dehydrogenase). Probe CYP3A4 substrates (midazolam at 3 μM and testosterone at 50 μM), were incubated for 10 and 30 min, respectively, with increasing concentrations of GM, MGM or CGM (concentration range: 1 - 30 μM). The reaction was stopped by adding acetonitrile to precipitate the proteins. The incubation mixtures were then centrifuged for 5 min at 10,000 × g, and an aliquot of the supernatant was analyzed using high-performance liquid chromatography coupled to mass spectrometry for the assessment of 1’-hydroxymidazolam and 6β-hydroxytestosterone metabolite formation.
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Publication 2023
acetonitrile Buffers Cytochrome P-450 CYP3A4 Donors Glucose-6-Phosphate Glucosephosphate Dehydrogenase High-Performance Liquid Chromatographies Homo sapiens Magnesium Chloride Mass Spectrometry Microsomes, Liver Midazolam NADP Phosphates Proteins Psychological Inhibition Testosterone

Top products related to «Testosterone»

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Testosterone is a laboratory equipment product that measures the concentration of the hormone testosterone in biological samples. It is used in research and clinical settings to assess testosterone levels for various purposes, such as evaluating hormonal imbalances or monitoring treatment effects.
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The Immulite 2000 is an automated immunoassay analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of immunoassay tests, including those for hormones, proteins, and other analytes. The system utilizes chemiluminescent technology for detection and provides automated sample handling, reagent management, and result reporting.
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Progesterone is a steroid hormone that plays a crucial role in the female reproductive system. It is a key component in the regulation of the menstrual cycle and supports the maintenance of pregnancy. Progesterone is commonly used in various lab equipment and scientific research applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Phenacetin is a chemical compound used in the manufacturing of various pharmaceutical and laboratory products. It serves as a key ingredient in the production process. Phenacetin has specific functional properties that make it a valuable component in relevant applications, but a detailed description of its core function is beyond the scope of this response.
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The Cobas e411 is a compact and fully automated immunoassay analyzer designed for clinical laboratory settings. It is capable of performing a wide range of immunoassay tests, including those for hormones, therapeutic drug monitoring, and infectious disease markers. The Cobas e411 is known for its reliability, ease of use, and efficient throughput.
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Testosterone is a steroid hormone that plays a crucial role in the development and maintenance of male sexual characteristics. It is a core product used in research and laboratory settings to study various biological processes related to male physiology and reproduction.
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6β-hydroxytestosterone is a synthetic steroid compound that is commonly used as a reference standard in analytical and clinical laboratory applications. It serves as a tool for identifying and quantifying the presence of this metabolite in various biological samples. This product is intended for research and development purposes only, and its specific uses should be evaluated by qualified professionals.
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Chlorzoxazone is a laboratory chemical used as a reference standard. It is a crystalline solid with a molecular formula of C7H5ClNO. Chlorzoxazone is primarily used for analytical purposes and quality control in various industries.
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Corticosterone is a laboratory reagent used in scientific research. It is a naturally occurring steroid hormone produced by the adrenal glands in various species. Corticosterone plays a role in the regulation of metabolism, immune function, and stress response. As a research tool, it is often utilized in studies involving endocrinology, neuroscience, and pharmacology.

More about "Testosterone"

Testosterone, a vital androgenic steroid hormone, plays a pivotal role in the development and maintenance of male sexual characteristics.
Produced primarily by the testes in men and the ovaries and adrenal glands in women, this essential compound regulates a wide range of physiological processes, including sexual function, muscle growth, bone density, and red blood cell production.
Researchers exploring the intricacies of testosterone may leverage the power of PubCompare.ai, an AI-driven platform that enables the optimization of their research efforts.
This innovative tool allows scientists to locate the best protocols from a vast array of literature, pre-prints, and patents, utilizing intelligent comparison techniques.
By harnessing the capabilities of PubCompare.ai, researchers can enhance the reproducibility and accuracy of their testosterone studies, empowering them to experience the future of testosterone research today.
The platform's powerful analysis tools provide invaluable insights, facilitating informed decision-making and driving advancements in the field.
Beyond testosterone, researchers may also explore related concepts such as Immulite 2000, a widely used automated immunoassay system; Progesterone, another crucial steroid hormone; FBS (Fetal Bovine Serum), a commonly used cell culture supplement; Phenacetin, a historical analgesic drug; Cobas e411, a popular automated immunoassay analyzer; 6β-hydroxytestosterone, a key metabolite of testosterone; Chlorzoxazone, a muscle relaxant; and Corticosterone, a glucocorticoid hormone.
By understanding these interconnected topics, researchers can gain a more comprehensive understanding of the complex physiological landscape surrounding testosterone.
Experence the future of testosterone research today with PubCompare.ai, and unlock the full potential of your research endeavors.