All reagents and anhydrous solvents used during the synthesis were of commercial quality. 5,11,17,23-Tetra-(t-butyl)-25-hydroxy-26,27,28-tripropoxy-calix[4]arene (6 ) was prepared according to the literature (Gutsche and Iqbal, 1990 (link)). The lower rim of the calix[4]arene backbone was modified in accordance to the described procedures for the propylation (7a,b) of the hydroxylic groups (Gutsche and Lin, 1986 (link)), as well as condensation of one of the hydroxyls (8a ) with N-(3-bromo)propylphthalimide (Lalor et al., 2007 (link)). The steps, preceding the final conjugation with the DOTA-units (Schühle et al., 2009 (link)) included nitration (9a,b ) of the upper rim of the calix[4]arene backbone (Kelderman et al., 1992 (link)) followed by the reduction (10a,b ) of the nitro groups to the amines (Klimentová and Vojtíšek, 2007 (link)). 1H NMR spectra were recorded at 25°C on Bruker Avance-400 spectrometer operating at 400.13 MHz and analyzed using Bruker™ TopSpin 2.1 software. The chemical shifts are reported in δ (ppm) using tetramethylsilane (TMS) as an internal reference. Ultra-filtration was performed with a Millipore stirred cell using an Amicon cellulose acetate membrane. All HPLC measurements were carried out on a Shimadzu LC-20 system consisting out of an LC-20AT pump, Sil-20A HT autosampler, CTO-20AC column oven, SPD-M20A PDA detector, CBM-20A controller, and a Waters Fraction Collector III; data processing was carried out using Shimadzu Lab Solutions. Both analytical and preparative methods were carried out operating at 40°C using eluents A: H2O (95%), AcCN (5%), TFA (0.1%) and B: H2O (5%), AcCN (95%), TFA (0.1%). Mobile phase gradient started with 75% A and 25% B, after 18 min followed by a change linear to 58% A and 42% B, after 2 min a change linear to 100% B, which was hold for 0.5 min and then chanced back to starting conditions stabilized for 3.5 min. Analytical measurements used a Waters Xterra 4.6 × 150 mm column and an injection volume of 1 μL, flow was 1 mL/min. Preparative HPLC was performed using Xbridge™ PrepShield RP18-OBD C18-19 × 150 mm column. Mass spectrometry analysis was done with electron spray ionization technique on Waters Qtof Premier MS using a NE-1000 syringe pump for direct infusion; data processing was carried out using Waters Masslynx. Qualitative luminescence measurements were done on a Jasco J815 CD spectrometer using 100 μL of sample in a 3 × 3 mm quartz cuvette. UV absorption spectra were measured on a UV2401 PC Shimadzu spectrometer. For quantitative luminescence measurements, the samples (either powders or solutions in Milli-Q water at concentration 2, 0.2, and 0.04 mM) were placed into 2.4 mm quartz capillaries and measured on a Horiba-Jobin-Yvon Fluorolog 3 spectrofluorimeter equipped with visible (220–800 nm, photon-counting unit R928P) and NIR (950–1,450 nm, photon-counting units H10330-45 from Hamamatsu or DSS-IGA020L Jobin-Yvon solid-state InGaAs detector, cooled to 77 K) detectors. All spectra were corrected for the instrumental functions. Luminescence lifetimes of TbIII-complexes were determined under excitation at 300 nm provided by a Xenon flash lamp monitoring the signal at 545 nm (5D4→7F5 transition). Quantum yields were measured according to an absolute method using an integration sphere (GMP SA). Each sample was measured several times under slightly different experimental conditions. Estimated experimental error for quantum yields determination is 10%. Nile red (NR) fluorescence measurements were performed on a Jasco J-815 CD spectrometer. The temperature was controlled using a Jasco PFD 4252/15 Peltier temperature unit. All samples contained Nile red in 2 μM concentrations and were excited at 550 nm. The maximum Nile red emission wavelength (λmax) was determined as a function of the calix[4]arene concentration.
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Tetramethylsilane
Tetramethylsilane
Tetramethylsilane is a colorless, volatile organic compound with the chemical formula Si(CH3)4.
It is commonly used as a standard reference compound in nuclear magnetic resonance (NMR) spectroscopy due to its sharp, singlet signal.
Tetramethylsilane is also employed as a silylaing reagent in organic synthesis and as a solvent in various chemical applications.
The PubCompare.ai platform can help researchers optimize and compare protocols for working with tetramethylslane, enabling more accurate and reproducible experiments.
It is commonly used as a standard reference compound in nuclear magnetic resonance (NMR) spectroscopy due to its sharp, singlet signal.
Tetramethylsilane is also employed as a silylaing reagent in organic synthesis and as a solvent in various chemical applications.
The PubCompare.ai platform can help researchers optimize and compare protocols for working with tetramethylslane, enabling more accurate and reproducible experiments.
Most cited protocols related to «Tetramethylsilane»
Acids
Benzene
Boronic Acids
Carbon-13 Magnetic Resonance Spectroscopy
diphenyl
Esters
Fingers
Gas Chromatography-Mass Spectrometry
Mass Spectrometry
Nexus
NMR, Multinuclear
Palladium
Phenols
Polychlorinated Biphenyls
Spectrometry, Mass, Electrospray Ionization
tetramethylsilane
triphenylphosphine
Workers
1H NMR
Alabaster
Anabolism
Chloroform
Chromatography
Ethyl Ether
Gel Chromatography
Glycerylphosphorylcholine
Lipids
Mass Spectrometry
n-hexane
Phospholipids
Silica Gel
Silicon Dioxide
Solvents
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
tetramethylsilane
Toluene
Reagents and solvents were purchased from Fischer Scientific, Sigma Aldrich™, Fluorochem™, or Alfa Aesar™, were of analytical reagent grade and were used as received. 1H and 13C-NMR spectra were recorded, in specified deuterated solvents, (purchased from Apollo Scientific™), at room temperature on Bruker™ Avance-400 (1H, 13C) (operating at 400.13 MHz) spectrometers and are reported as follows: chemical shift δ (ppm) (number of protons, multiplicity, coupling constant J (Hz) (if applicable), assignment). Multiplicities are reported using the following abbreviations: s (singlet), d (doublet), t (triplet), q (quartet) and m (unresolved multiplet). All 13C-NMR spectra were proton-decoupled and carbons are numbered according to the IUPAC systematic name. The 1H and 13C chemical shifts are reported using the residual signal of deuterated solvent as the internal reference (for CDCl3: δH = 7.26 ppm; δC = 77.16 ppm and for deuterated d6-DMSO: δH = 2.50 ppm; δC = 39.51 ppm). All chemical shifts are quoted in parts per million, relative to tetramethylsilane (δH, δC = 0.00 ppm). All coupling constants are 3JHH unless otherwise stated. Electrospray Ionisation (ESI) mass spectra, for LC-MS results were obtained using a TQD mass spectrometer (Waters UK Ltd., Manchester, UK). High-resolution mass spectra were obtained with an LCT Premier XE mass spectrometer (Waters UK Ltd., Manchester, UK); all were obtained by the Durham University Mass Spectrometry service. ASAP mass spectra were obtained using a Waters™ Synapt G2s apparatus. Thin-Layer Chromatography was performed using Merck TLC Aluminium oxide 60 F254 with glass backing. Plates were stained with potassium permanganate solution, where required and visualised using UV light. Column chromatography refers to purification by applying the mixture, dissolved in a minimum amount of dichloromethane, onto silica gel (40–63 µm mesh size) with the stated solvent system.
Carbon
Carbon-13 Magnetic Resonance Spectroscopy
Chromatography
Mass Spectrometry
Methylene Chloride
Oxide, Aluminum
Potassium Permanganate
Protons
Silica Gel
Solvents
Spectrometry, Mass, Electrospray Ionization
Sulfoxide, Dimethyl
tetramethylsilane
Thin Layer Chromatography
Triplets
Ultraviolet Rays
Chemistry All chemical compounds were purchased from commercial suppliers of Merck and Aldrich companies. The purity of the prepared compounds was proved by thin layer chromatography (TLC) using various solvents of different polarities. Merck silica gel 60 F254 plates were applied for analytical TLC. Column chromatography was performed on Merck silica gel (70-230 mesh) for purification obtained compounds. 1H-NMR spectra were recorded using a Brucker 200 MHz spectrometer, and chemical shifts were expressed as δ (ppm) with tetramethylsilane (TMS) as internal standard. The IR spectra were obtained on a Shimadzu 470 spectrophotometer (potassium bromide disks). Melting points were determined using electrothermal melting point analyzer apparatus and were uncorrected. The mass spectra were run on a Finigan TSQ-70 spectrometer (Finigan, USA) at 70 eV. All cell lines were purchased from the Pasteur Institute of Iran.
According to the
Synthesis of N-(5-Mercapto-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (2)In a flask, equimolar amounts of 4-trifluoromethylphenylacetic acid, EDC and HOBtin acetonitrile solvent were stirred for 30 min and then equimolar quantity of 5-amino-1,3,4-thiadiazole-2-thiol was added. The stirring condition was continued for 24 h. The end point of the reaction was determined by thin layer chromatography(TLC). Acetonitrile was removed under reduced pressure and ethyl acetate/water was added. The aqueous layer was removed and organic layer was washed two times by sodium bicarbonate 5%, diluted sulfuric acid and brine. Anhydrous sodium sulfate was added for drying and then filtered. The ethyl acetate was evaporated using rotary evaporator apparatus. The obtained yellowish solid was washed by dry ether and used for the next step.
mp. 171°C, Yield: 65%,C11H8F3N3OS2, MW: 319 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 3.76 (s, 2H, -CH2CO-), 3.95 (s, 1H, -SH), 7.58 (m, 2H, J = 8Hz), 8.19 (m, 2H, J = 8Hz, 4-trifluoromethylphenyl), 12.76 (brs, 1H, NH).IR (KBr, cm-1) ῡ: 3265, 1697, 1580, 1519, 1321, 1155, 1103, 1064, 821, 705.MS(m/z, %): M++2: 322(10), M+: 320(10), 279(45), 276(35), 167(80), 159(95), 149(100), 133(15), 109(15), 71(15), 57(20).
General procedure for synthesis of compounds 3a-3lEquimolar quantities of appropriate benzyl chloride derivative was treated with 2-(4-fluorophenyl)-N-(5-mercapto-1,3,4-thiadiazol-2-yl)acetamide (2 (link)). Equimolar amount of potassium hydroxide in absolute ethanol was added to convert the thiol moiety to the thiolate anion. Then, the related benzyl chloride derivative was added to the reaction medium and reflux condition was performed for 24 h. Crushed ice was added and the precipitate filtered, washed by cool water and purified by appropriate procedures such as crystallization or column chromatography (EtOAC/Petroleum ether: 3/2).
N-(5-(2-Fluorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3a)mp. 201 °C, Yield: 42%,C18H13F4N3OS2, MW: 427 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 4.00 (s, 2H, -CH2CO-), 4.53 (s, 2H, -CH2S-), 7.16-7.54 (m, 2-fluorobenzyl), 7.59 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.76 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 13.00 (s, NH). IR(KBr, cm-1) ῡ: 3158, 3050, 2898, 1690, 1560, 1493, 1456, 1421, 1402, 1337, 1301, 1236, 1168, 1117, 1071, 1019, 972, 844, 757, 693, 655.MS(m/z, %): M+: 427(95), 377(60), 325(35), 297(30), 236(25), 236(15), 159(75), 109(100).
N-(5-(3-Fluorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3b)mp. 190 °C, Yield: 58%,C18H13F4N3OS2, MW: 427 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 3.93 (s, 2H, -CH2CO-), 4.53 (s, 2H, -CH2S-), 7.01-7.47 (m, 3-fluorobenzyl), 7.58 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.98 (s, NH).IR(KBr, cm-1) ῡ: 3448, 3156, 2916, 1699, 1562, 1488, 1358, 1326, 1172, 1110, 1068, 837.MS(m/z, %): M+: 427(85), 377(80), 325(40), 297(25), 236(20), 159(70), 109(100).
N-(5-(4-Fluorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3c)mp. 166 °C, Yield: 40%,C18H13F4N3OS2, MW: 427 g/mol,1H NMR (DMSO-d6 , 200 MHz) δ: 3.99 (s, 2H, -CH2CO-), 4.51 (s, 2H, -S-CH2-), 7.24 (t, 2H, 4-fluorobenzyl), 7.48 (t, 2H, 4-fluorobenzyl), 7.58 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.97 (s, NH).IR(KBr, cm-1) ῡ: 3370, 3030, 2920, 2829, 1701, 1558, 1508, 1323, 1219, 1160, 1109, 1060, 835.MS(m/z, %): M+: 427(90), 377(75), 325(40), 297(30), 236(25), 159(75), 109(100).
N-(5-(2-Chlorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3d)mp. 208 °C, Yield: 54%,C18H13ClF3N3OS2, MW: 443 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 4.00 (s, 2H, -CH2CO-), 4.59 (s, 2H, -CH2S-), 7.35 (m, 2-chlorophenyl), 7.48 (d, 2H, J = 12 Hz, 4-trifluoromethylphenyl), 7.55 (m, 2-chlorophenyl), 7.97(d, 2H, J = 12 Hz, 4-trifluoromethylphenyl), 13.00 (s, NH). IR(KBr, cm-1) ῡ: 3447, 3165, 2922, 1700, 1570, 1445, 1326, 1171, 1109, 1067, 868, 759.MS(m/z, %): M+:443(10), 410(55), 409(85), 159(40), 127(50), 125(100), 109(10), 89(10).
N-(5-(3-Chlorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3e)mp. 198 °C, Yield: 57%,C18H13ClF3N3OS2, MW: 443 g/mol,1H NMR (DMSO-d6 , 200 MHz) δ: 4.00 (s, 2H, -CH2CO-), 4.59 (s, 2H, -S-CH2-), 7.33-7.40 (m, 3-chlorobenzyl), 7.43 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.56 (m, 3-chlorobenzyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 13.00 (s, NH).IR(KBr, cm-1) ῡ: 3427, 3165, 2914, 2730, 1700, 1569, 1446, 1356, 1326, 1301, 1170, 1112, 1066, 868, 839, 759.MS(m/z, %): M+:443(15), 410(60), 409(65), 282(10), 159(55), 127(35), 125(100), 89(10).
N-(5-(4-Chlorobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3f)mp. 198 °C, Yield: 34%,C18H13ClF3N3OS2, MW: 443 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 3.99 (s, 2H, -CH2CO-), 4.52 (s, 2H, -S-CH2-), 7.38-7.48 (m, 4-chlorobenzyl), 7.58 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.78 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.97 (s, NH).IR(KBr, cm-1) ῡ: 3440, 3130, 3040, 2877, 1690, 1556, 1334, 1163, 1118, 1068, 1020, 846, 745, 702.MS(m/z, %): M++1: 444(10), M+:443(10), 410(60), 409(80), 282(12), 178(12), 159(40), 127(50), 125(100), 109(10), 89(10).
N-(5-(3-Methoxybenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3g)mp. 160 °C, Yield: 32%,C19H16F3N3O2S2, MW: 439 g/mol,1H NMR (DMSO-d6 , 200 MHz) δ:1H NMR (DMSO-d6 , 200 MHz) δ: 3.80 (s, 3H, -OCH3), 3.99 (s, 2H, -CH2CO-), 4.48 (s, 2H, -S-CH2-),7.06(m, 3H, 3-methoxybenzyl), 7.32(m, 1H, 3-methoxybenzyl), 7.58(d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.97 (s, NH).IR(KBr, cm-1) ῡ:3167, 3038,2946, 1735, 1702, 1607, 1577, 1488, 1438, 1358, 1325, 1302, 1271, 1158, 1113, 1068, 839, 777, 736.MS(m/z, %): M+: 439(40), 159(75), 122(45), 121(100), 109(35).
N-(5-(4-Methoxybenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3h)mp. 219 °C, Yield: 36%,C19H16F3N3O2S2, MW: 439 g/mol,1H NMR (DMSO-d6 , 200 MHz) δ: 3.76 (s, 3H, -OCH3), 3.99 (s, 2H, -CH2CO-), 4.46 (s, 2H, -S-CH2-), 6.92 (d, 2H, J = 8 Hz, 4-methoxybenzyl), 7.35 (d, 2H, J = 8 Hz, 4-methoxybenzyl), 7.58 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.96 (s, NH).IR(KBr, cm-1) ῡ: 3265, 3045, 2870, 1691, 1554, 1510, 1400, 1332, 1298, 1170, 1122, 1107, 1066, 827. MS(m/z, %): M++2: 441(15), M++1: 440(20), M+: 439(25), 159(60), 122(45), 121(100), 109(30).
N-(5-(2-Nitrobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3i)mp. 146 °C, Yield: 35%,C18H13F3N4O3S2, MW: 454 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 4.00 (s, 2H, -CH2CO-), 4.79 (s, 2H, -CH2S-), 7.49 (m, aromatic), 8.11 (m, aromatic), 8.21 (m, aromatic), 13.00 (s, NH). IR(KBr, cm-1) ῡ: 3447, 3165, 2923, 1698, 1612, 1573, 1527, 1443, 1335, 1168, 1106, 1066, 830, 827, 702.MS(m/z,%): M+: 454(15), 270(35), 242(65), 225(15), 195(100), 179(65), 165(85), 136(70), 106(45), 90(50), 78(35).
N-(5-(3-Nitrobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3j)mp. 132 °C, Yield: 45%,C18H13F3N4O3S2, MW: 454 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 3.84 (s, 2H, -CH2CO-), 4.51 (s, 2H, -CH2S-), 7.71(m, 5H, aromatic), 8.16(m, 3H, aromatic), 13(brs, NH).IR(KBr, cm-1) ῡ: 3318, 3154, 2850, 1692, 1629, 1562, 1527, 1508, 1487, 1348, 1329, 1163, 1117, 1073, 810, 747.MS(m/z,%): M++1: 455(7), M+: 454(15), 270(30), 242(75), 195(100), 179(70), 165(90), 136(60), 106(35), 90(45), 78(60).
N-(5-(4-Nitrobenzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3k)mp. 198 °C, Yield: 54%,C18H13F3N4O3S2, MW: 454 g/mol,1H NMR (DMSO-d6, 200 MHz) δ: 3.99 (s, 2H, -CH2CO-), 4.66 (s, 2H, -S-CH2-),7.55(m, 2H, aromatic), 7.77(m, 4H, aromatic), 8.25(m, 2H, aromatic), 12.99(brs, NH).IR(KBr, cm-1) ῡ:3265, 3153, 3040, 2912, 2852, 1697, 1554, 1517, 1342, 1323, 1155, 1103, 1064, 1020, 960, 830.MS(m/z,%): M++1: 455(10), M+: 454(10), 270(40), 242(75), 225(40), 195(100), 179(95), 165(85), 136(60), 106(50), 90(50), 78(60).
N-(5-(Benzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide (3l)mp. 203 °C, Yield: 47%,C18H14F3N3OS2, MW: 409 g/mol,1H NMR (DMSO-d6 , 200 MHz) δ: 3.99 (s, 2H, -CH2CO-), 4.52 (s, 2H, -S-CH2-), 7.30-7.49 (m, 5H, benzyl), 7.58 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 7.75 (d, 2H, J = 8 Hz, 4-trifluoromethylphenyl), 12.97 (s, NH).IR(KBr, cm-1) ῡ: 3375, 3040, 1701, 1556, 1508, 1323, 1294, 1220, 1159, 1107, 1066, 1020, 827, 700.MS(m/z, %): M+: 410(100), 409(95), 408(95), 159(75), 148(60), 91(75).
MTT assayDiverse derivatives of 1,3,4-thiadiazole (compounds 3a-3l) were tested for cytotoxic activity at 0.1-250 μg/mL concentration in three human cancer cell lines of PC3 cell (prostate cancer), U87 (gliobalstoma) and MDA (breast cancer). Cells from different cell lines were seeded in 96-well plates at the density of 8000–10,000 viable cells per well and incubated for 48 h to allow cell attachment.The cells were then incubated for another 48-96 h (depends to cell cycle of each cell line) with various concentrations of compounds 3a-3l. Cells were then washed inPBS, and 20 μL of MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide solution (5 mg/mL) were added to each well. An additional 4 h of incubation at 37°C were done, and then the medium was discarded. Dimethyl sulfoxide (60 μL) was added to each well, and the solution was vigorously mixed to dissolve the purple tetrazolium crystals. The absorbance of each well was measured by plate reader (Anthous 2020; Austria) at a test wavelength of 550 nm against a standard reference solution at 690 nm. The amount of produced purple formazan is proportional to the number of viable cells (16 (link)).
Most recents protocols related to «Tetramethylsilane»
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1H NMR spectra were obtained on a Bruker Avance 300 MHz spectrometer (Germany). All samples were dissolved in dimethyl sulfoxide (DMSO-d6). The internal standard was tetramethylsilane (TMS).
The NMR experiment was performed on a BRUKER AVANCE III 600 MHz NMR spectrometer with an experimental temperature of 298 K, and a solvent of deuterated chloroform. The internal standard was tetramethylsilane (TMS).
The chemical characteristics of the metabolites were investigated using gas chromatography-mass spectrometry (GC-MS), infrared spectroscopy (IR), nuclear magnetic resonance (NMR) spectroscopy, and referencing of chemical shifts to tetramethylsilane (TMS Oxoid, UK) as an international standard.
1H and 13C NMR spectra for the samples dissolved in chloroform-d (with tetramethylsilane internal standard) were collected using a Bruker Ascend 600 MHz spectrometer. For measurements, standard experimental conditions and the standard Bruker program were applied.
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The Avance 400 is a nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It is designed to analyze the chemical composition and structure of materials through the detection and measurement of nuclear magnetic resonances.
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The 400 MHz spectrometer is a laboratory instrument used for nuclear magnetic resonance (NMR) spectroscopy. It operates at a frequency of 400 MHz, which is a commonly used field strength for NMR analysis. The spectrometer is designed to detect and measure the magnetic properties of atomic nuclei within a sample, providing information about the chemical structure and composition of the sample.
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The Avance 400 spectrometer is a nuclear magnetic resonance (NMR) instrument manufactured by Bruker. It is designed to analyze the chemical structure and composition of samples using the principles of NMR spectroscopy. The Avance 400 spectrometer operates at a frequency of 400 MHz and is capable of performing a wide range of NMR experiments to study a variety of sample types.
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The Avance 500 is a high-performance nuclear magnetic resonance (NMR) spectrometer manufactured by Bruker. It provides advanced analytical capabilities for chemical and biological research applications.
More about "Tetramethylsilane"
Tetramethylsilane (TMS) is a colorless, volatile organic compound with the chemical formula Si(CH3)4.
It is a widely used standard reference compound in nuclear magnetic resonance (NMR) spectroscopy, known for its sharp, singlet signal.
TMS is also employed as a silylation reagent in organic synthesis and as a solvent in various chemical applications.
Researchers can optimize and compare protocols for working with tetramethylsilane using the PubCompare.ai platform.
This AI-driven tool helps locate the most accurate and reproducible protocols from literature, preprints, and patents, enabling researchers to identify the best approach for their experiments.
When conducting NMR analysis, TMS is often used in conjunction with silica gel 60 F254 as a thin-layer chromatography (TLC) adsorbent, and Avance 400 or AV-400 spectrometers operating at 400 MHz for high-resolution NMR measurements.
The Avance III and Avance 500 spectrometers are also commonly used for TMS-based NMR studies.
By utilizing the PubCompare.ai platform, researchers can streamline their research on tetramethylsilane, optimizing experimental protocols and ensuring more accurate and reproducible results.
This comprehensive approach to protocol optimization and product selection empowers researchers to make informed decisions and advance their scientific investigations.
It is a widely used standard reference compound in nuclear magnetic resonance (NMR) spectroscopy, known for its sharp, singlet signal.
TMS is also employed as a silylation reagent in organic synthesis and as a solvent in various chemical applications.
Researchers can optimize and compare protocols for working with tetramethylsilane using the PubCompare.ai platform.
This AI-driven tool helps locate the most accurate and reproducible protocols from literature, preprints, and patents, enabling researchers to identify the best approach for their experiments.
When conducting NMR analysis, TMS is often used in conjunction with silica gel 60 F254 as a thin-layer chromatography (TLC) adsorbent, and Avance 400 or AV-400 spectrometers operating at 400 MHz for high-resolution NMR measurements.
The Avance III and Avance 500 spectrometers are also commonly used for TMS-based NMR studies.
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