Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Tetramisole

Tetramisole

Tetramisole is an anthelmintic drug used to treat parasitic infections.
It acts by inhibiting the enzyme acetylcholinesterase, leading to spasmodic paralysis and expulsion of the parasite.
Tetramisole is commonly used to treat roundworm and hookworm infections in both humans and animals.
Researchers can optimzie their Tetramisole studies using PubCompare.ai, an AI-driven platform that helps identify the best protocols and products to enhance reproducibility and accuracy.
Leveraging advanced technoques, PubCompare.ai allows users to easily locate and compare Tetramisole protocols from literature, preprints, and patents, taking your research to new hights.
Experince the power of AI-driven protocol comparison today.

Most cited protocols related to «Tetramisole»

1
Imaging Kinesin Dynamics in C. elegans
Cited 36 times
Microscopy images were acquired using a custom-built epi-illuminated wide-field fluorescence microscope operated by a MicroManager software interface (μManager, MicroManager 1.4, www.micromanager.org; Edelstein et al. 2014 ) and built around an inverted microscope body (Eclipse Ti; Nikon, Amsterdam, Netherlands) fitted with a 60× water-immersion objective (CFI Plan Apo IR 60× water immersion, numerical aperture 1.27; Nikon). Excitation light was provided by a diode-pumped solid-state laser (Calypso 50, 491 nm; Cobolt, Solna, Sweden). Images were captured with an electron-multiplying charge-coupled device camera (iXon 897; Andor, Belfast, UK). One camera pixel corresponded to 92 nm × 92 nm in the image plane.
The C. elegans strain expressing EGFP-tagged OSM-3 kinesin motor proteins (Snow et al. 2004 (link)) was a kind gift of Jonathan M. Scholey (University of California, Davis, Davis, CA). Fluorescence imaging in living C. elegans was performed by anesthetizing adult worms (maintained at 20°C) in M9 containing 5 mM levamisole (tetramisole hydrochloride, L9756; Sigma-Aldrich, St. Louis, MO) and immobilizing them between a 2% agarose pad and a coverslip. Samples were imaged at room temperature (21°C) at 152 ms/frame.
Mangeol P., Prevo B, & Peterman E.J. (2016). KymographClear and KymographDirect: two tools for the automated quantitative analysis of molecular and cellular dynamics using kymographs. Molecular Biology of the Cell, 27(12), 1948-1957.
Publication 2016
Adult Electrons Fluorescence Helminths Kinesin Levamisole Light Light Microscopy Medical Devices Microscopy Microscopy, Fluorescence OSM-3 protein, C elegans Reading Frames Sepharose Snow Somatotype Strains Submersion Tetramisole thiacloprid
2
ATP-induced Phosphate Release Assay
Cited 6 times
5 × 104 CD4+/CD25+ or CD4+/CD25− cells were isolated and washed three times in cold phosphate-free buffer. Cells were warmed in incubation buffer (10 mM glucose, 20 mM Hepes, pH 7.5, 5 mM KCl, 120 mM NaCl, 2 mM CaCl2, and 5 mM tetramisole) to 37°C for 10 min. Cells were then incubated in the same buffer with 2 mM ATP for 10 min. Reactions were stopped with the addition of trichloroacetic acid to a final concentration of 5% and immediately put on ice. Phosphate concentration was measured after the addition of Malachite green/polyvinyl alcohol/ammonium molybdate solution for 20 min by a spectrophotometer (ELx808 Ultra Microplate Reader; Bio-Tek Instruments, Inc.) at 610 nm and compared against a standard curve.
Deaglio S., Dwyer K.M., Gao W., Friedman D., Usheva A., Erat A., Chen J.F., Enjyoji K., Linden J., Oukka M., Kuchroo V.K., Strom T.B, & Robson S.C. (2007). Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression. The Journal of Experimental Medicine, 204(6), 1257-1265.
Publication 2007
ammonium molybdate Buffers CD4 Positive T Lymphocytes Cells Cold Temperature Glucose HEPES IL2RA protein, human malachite green Phosphates Polyvinyl Alcohol Sodium Chloride Tetramisole Trichloroacetic Acid
3
Mitochondrial and Peroxisome Imaging in C. elegans
Cited 5 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2017
Cell Nucleus Cells Helminths Intestinal helminthiasis Intestines Microscopy Mitochondria Mitochondria, Muscle Mitochondrial Inheritance Mitochondrial Membrane, Outer Muscle Tissue Peroxisome Pharynx Sepharose Strains Tail Tetramisole Vision Vulva
4
Calcium Imaging of ASH and AWC Neurons
Cited 5 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2014
Adult Calcium Cells Cholinergic Agonists Dimethylpolysiloxanes Flicker Fusion Fluorescein Fluorescence Fluorescent Dyes Glycerin Helminths Microchip Analytical Devices Microscopy Movement Neurons Nose Obstetric Delivery Odors Polymers Pressure Pulse Rate Pulses Reading Frames Tetramisole
5
Laser Axotomy and Axon Regrowth Quantification
Cited 5 times
Animals were anaesthetized on agar pads using 0.01–0.05% Tetramisole. We performed laser axotomy using a MicroPoint Laser System Basic Unit attached to a Zeiss Axio Imager A1 (Objective EC Plan-Neofluar 100x/1.30 Oil M27). This laser delivers 120 μJoules of 337 nm energy with a 2 – 6 nsec pulse length. Axotomies were completed with 20 to 30 pulses on larval stage 4 animals at a point approximately 50 μm anterior to the ALM, PLM and PLN cell bodies, and approximately 500 μm from the cell body of ALN. Animals were analyzed with a Zeiss Axio Imager Z1 equipped with a Photometrics Cool Snap HQ2 camera and analysis was performed using Metamorph software. Axon regrowth was quantified by measuring the length of the longest ALM or PLM process beyond the cut site 24 hrs post-axotomy; neurons that underwent axonal fusion were excluded from these quantifications.
Neumann B., Nguyen K.C., Hall D.H., Ben-Yakar A, & Hilliard M.A. (2011). Axonal regeneration proceeds through specific axonal fusion in transected C. elegans neurons. Developmental dynamics : an official publication of the American Association of Anatomists, 240(6), 1365-1372.
Publication 2011
Agar Animals Axon Cell Body Larva Neurons Pulse Rate Pulses Tetramisole

Most recents protocols related to «Tetramisole»

1
Imaging AP-3 localization in C. elegans
L4 or 1-day adult worms were immobilized using 30 mM sodium azide and mounted on 2–5% agarose pads. Images were acquired on an Olympus IX73 Epifluorescence microscope with an Andor EMCCD camera or the Olympus Fluoview FV1000 confocal laser scanning microscope or Olympus IX83 with Perkin Elmer Ultraview Spinning Disc confocal microscope fitted with a Hamamatsu EMCCD camera. Since AP-3 localization is sensitive to levels of ATP (Faundez and Kelly, 2000 (link)), static imaging of APB-3::GFP was performed using 5 mM Tetramisole. APB-3::GFP was imaged on Olympus Spin SR10 (SoRA, 50 μm disk) fitted with Teledyne Photometrics sCMOS camera.
Nadiminti S.S., Dixit S.B., Ratnakaran N., Hegde S., Swords S., Grant B.D, & Koushika S.P. (2023). Active zone protein SYD-2/Liprin-α acts downstream of LRK-1/LRRK2 to regulate polarized trafficking of synaptic vesicle precursors through clathrin adaptor protein complexes. bioRxiv.
Publication Preprint 2023
Adult Helminths Microscopy Microscopy, Confocal Microscopy, Confocal, Laser Scanning Sepharose Sodium Azide Tetramisole
2
Visualizing Neuronal Microtubule Dynamics in C. elegans
L4 worms were anesthetized in 3 mM tetramisole (Sigma-Aldrich) and mounted on 5% agarose pads. Time-lapse images were acquired in Olympus IX83 with Perkin Elmer Ultraview Spinning Disc confocal microscope and a Hamamatsu EMCCD camera or the Olympus Fluoview FV1000 confocal laser scanning microscope. Dual color simultaneous imaging was performed at 3 frames per second (fps), dual color sequential imaging was done at 1.3 fps, and single fluorophore imaging for analysis of vesicle length was done at 5 fps. All movies were 3 minutes long, and the region of imaging in the PLM comprised the first 60–100 μm of the neuronal process immediately outside the cell body, with the cell body in the frame of imaging. Live imaging of EBP-2::GFP to assess microtubule polarity was carried out using an Olympus IX73 Epifluorescence microscope with an Andor EMCCD camera at 3 fps.
Nadiminti S.S., Dixit S.B., Ratnakaran N., Hegde S., Swords S., Grant B.D, & Koushika S.P. (2023). Active zone protein SYD-2/Liprin-α acts downstream of LRK-1/LRRK2 to regulate polarized trafficking of synaptic vesicle precursors through clathrin adaptor protein complexes. bioRxiv.
Publication Preprint 2023
Cell Body Helminths Microscopy Microscopy, Confocal Microtubules Neurites Reading Frames Sepharose Tetramisole
3
Quantifying Bacterial Colonization in C. elegans
The C. elegans strain N2 was used for all in vivo experiments and was originally obtained from the Caenorhabditis Genetics Center (CGC). Worms were maintained on NGM plates inoculated with Escherichia coli OP50 at 20 °C following standard procedures 50 (link). Assay plates were prepared by adding L-serine at a concentration of 0 mM, 10 mM, or 100 mM to NGM. Overnight cultures of MYb11 and MYb71 were adjusted to OD600 = 2.0 in PBS. 30-40 synchronized first instar larvae (L1) were grown on supplemented NGM plates inoculated with 125 μl of respective mono- or co-culture of MYb11 and MYb71. After 72 hours, bacterial lawns were sampled by pressing the circular end of a 200 μl pipette tip into the lawn, avoiding the edges and any worms. The resulting disc was homogenized in PBS with zirconia beads in a Bead Ruptor 96 (Omni International) for 3 minutes at 30 Hz.
To assess the number of CFU, worms were washed off plates using M9-buffer + 0.025% Triton X-100, washed 5 times and paralyzed with 5 mM tetramisole to stop pumping of the worms as described in 22 (link). Worms were surface-sterilized following 22 (link), then washed 2 times in PBS to remove residual bleach. Worms were transferred to a new tube to determine the exact worm number (~20) and PBS was added to a final volume of 400 ml. Worms were allowed to settle and 100 μl supernatant was collected (supernatant control). Worms were homogenized in the remaining PBS using 1-mm zirconia beads in a Bead Ruptor 96 for 3 minutes at 30 Hz. Homogenized worms, lawns, and supernatants were serially diluted in PBS and plated on TSA plates. After up to 48 h at 25 °C, the colonies were scored at the appropriate dilutions and the CFUs per worm were calculated. Each colonization experiment was conducted in three independent runs and equal biological replicates. Data were pooled across runs, and a mixed model controlling for runs was used to assess the effect of L-serine supplementation on bacterial colonization in C. elegans. For all L-serine in vitro and in vivo experiments, statistical comparisons were performed with generalized linear models, if not otherwise mentioned. We calculated the expected proportion of MYb11 in worms as the product of MYb11 proportions available on the lawns and the observed shift in bacterial proportions due to host filtering:
E(Prop.MYb11insupplementedworms)=(Prop.MYb11onsupplementedlawns)∗Prop.MYb11inwormsProp.MYb11onlawns
Plots and statistical analysis were produced with R studio (version 4.1.3) and edited with Inkscape (version 1.1.2).
Marinos G., Hamerich I.K., Debray R., Obeng N., Petersen C., Taubenheim J., Zimmermann J., Blackburn D., Samuel B.S., Dierking K., Franke A., Laudes M., Waschina S., Schulenburg H, & Kaleta C. (2023). Metabolic model predictions enable targeted microbiome manipulation through precision prebiotics. bioRxiv.
Full text: Click here
Publication Preprint 2023
Bacteria Biological Assay Biopharmaceuticals Buffers Caenorhabditis Caenorhabditis elegans Coculture Techniques Escherichia coli Helminths Larva Serine Technique, Dilution Tetramisole Triton X-100 zirconium oxide
4
Assessing Temperature-Sensitive Developmental Defects in swsn-1 Mutants
To assess temperature-sensitive developmental defects of different swsn-1 mutants and wild-type animals, animals were synchronized by bleaching as described in the section "Bleaching and Synchronization of C. elegans." To assess developmental differences between swsn-1 single and swsn-1; snfc-5 double mutants, recovered embryos were directly seeded onto NGM agar plates and grown at 25 °C for 48 h (SI Appendix, Fig. S1 D–F). To assess developmental differences between swsn-1 single and swsn-1; ubr-5 double mutants, recovered embryos were directly seeded onto NGM agar plates and grown at 22.5 °C for 48 h and 25 °C for 24 h (Fig. 1 D and E and SI Appendix, Fig. S1K). To assess developmental differences between swsn-1; snfc-5 double mutants and swsn-1; snfc-5; ubr-5 triple mutants, more stringent conditions were used. Recovered embryos were directly seeded onto NGM agar plates, grown at 15 °C until the L4 or young adult stage, and then shifted to 25 °C for 16 h. Gravid adults were bleached, and recovered embryos were seeded onto new NGM agar plates and grown at 25 °C for 72 h (Figs. 1 A and B and 2 B and C and SI Appendix, Figs. S1H and S2).
Animals were classified into categories (L1 or > L1, < gravid adult or gravid adult, and < L4 or L4 +, respectively) and manually counted (three replicates of at least 100 animals per genotype were counted). Subsequently, animals were washed off plates, collected in 1.5-mL tubes with M9 buffer, washed twice in 1 mL M9, pelleted, and M9 buffer-aspirated. To image animals, 18 µL of animals in M9 were deposited onto a glass slide coated with a 2% agarose pad and paralyzed by adding 2 µL 100 mM tetramisole. A coverslip was added and mounted with transparent nail polish. Representative differential interference contrast (DIC) microscopy images of animals were taken with a Leica DM6B microscope (upright microscope with the LAS X imaging system) at 10× magnification and 30-ms exposure time or 4x magnification and 10-ms exposure time.
Lampersberger L., Conte F., Ghosh S., Xiao Y., Price J., Jordan D., Matus D.Q., Sarkies P., Beli P., Miska E.A, & Burton N.O. (2023). Loss of the E3 ubiquitin ligases UBR-5 or HECD-1 restores Caenorhabditis elegans development in the absence of SWI/SNF function. Proceedings of the National Academy of Sciences of the United States of America, 120(5), e2217992120.
Full text: Click here
Publication 2023
Adult Agar Animals Animals, Wild Buffers Congenital Abnormality Embryo Figs Genotype Microscopy Microscopy, Differential Interference Contrast Nails Sepharose Tetramisole Young Adult
5
Dietary Restriction Imaging in C. elegans
Approximately 200 Day 1 adult worms in liquid culture were washed three times with SB media and moved to a flask containing 4 ml of SB media with a reduced food concentration to induce DR (109, 108 or 0 OP50 cells/ml) for a fixed amount of time (6, 12, or 24 h). After the DR period concluded, additional OP50 was added to raise the concentration to an ad libitum level (1010 OP50 cells/ml). FUDR concentration was raised to 100 µM total concentration to prevent offspring production69 (link). Worms were then kept at 20 °C for 3 days at ad libitum food concentration. DR exposure was repeated on Days 4, 7, and 10 of adulthood. At the start and end of each DR exposure, ~20–30 worms were immobilized using 10 µL of 2 mM tetramisole on dried 2% agarose pads of ~1 cm diameter. Confocal fluorescence microscopy was performed with a Leica DMi8 microscope paired with an 89 North LDI Laser Diode Illuminator coupled with a CrestOptics X-Light V2 confocal imager and an Orca-Flash 4.0 digital CMOS camera. The exposure time and laser intensity were kept constant throughout all experiments at 100 ms and 100%, respectively. Images containing 30 slices (Z-stacks with 3 µm spacing) were acquired with a MATLAB GUI (Graphical User Interface). Images were acquired in the green and red channels sequentially. This enabled later subtraction of autofluorescence present in both channels with a custom MATLAB script.
Huayta J., Crapster J.P, & San-Miguel A. (2023). Endogenous DAF-16 spatiotemporal activity quantitatively predicts lifespan extension induced by dietary restriction. Communications Biology, 6, 203.
Full text: Click here
Publication 2023
Adult Cells Chronic multifocal osteomyelitis Fingers Fluorescence Food Helminths Lasers, Semiconductor Light Microscopy Microscopy, Confocal Microscopy, Fluorescence Orcinus orca Sepharose Tetramisole

Top products related to «Tetramisole»

1
Tetramisole hydrochloride by Merck Group
Sourced in United States
Tetramisole hydrochloride is a chemical compound used as a laboratory reagent. It is a synthetic organic compound with the chemical formula C11H12Cl2N2. Tetramisole hydrochloride is commonly used in various scientific and research applications.
2
Tetramisole by Merck Group
Sourced in United States
Tetramisole is a laboratory instrument used for the detection and identification of helminth eggs in fecal samples. It is a centrifugal flotation device that separates and concentrates parasite eggs from fecal matter, enabling their visual examination under a microscope.
3
Axio imager m2 microscope by Zeiss
Sourced in Germany, United States, France, Canada, United Kingdom
The Axio Imager M2 is a high-performance microscope designed for advanced imaging and analysis. It features a stable and ergonomic design, offering a range of optical and illumination options to suit various applications. The microscope is equipped with advanced imaging capabilities, ensuring high-quality data acquisition and precise results.
4
Poly l lysine (pll) by Merck Group
Sourced in United States, Germany, United Kingdom, France, Japan, Sao Tome and Principe, Italy, Canada, China, Australia, Spain, Macao, Israel, Austria, Belgium, Denmark, Switzerland, Senegal, Hungary, Sweden, Ireland, Netherlands
Poly-L-lysine is a synthetic polymer composed of the amino acid L-lysine. It is commonly used as a coating agent for various laboratory applications, such as cell culture and microscopy. Poly-L-lysine enhances the attachment and growth of cells on surfaces by providing a positively charged substrate.
5
Axioscope by Zeiss
Sourced in Germany, United States, Italy
The Axioscope is a high-quality optical microscope designed for a wide range of applications in research and industrial settings. It features advanced optics, precision mechanics, and a modular design to accommodate various accessories and configurations. The Axioscope is a versatile instrument that provides reliable and consistent imaging performance.
6
Axiocam camera by Zeiss
Sourced in Germany, United States, Canada, United Kingdom, France, Japan, Belgium
The AxioCam is a high-performance digital camera designed for use with Zeiss microscopes. It features a large sensor size, high resolution, and advanced image processing capabilities to capture detailed, high-quality images of microscopic samples.
7
Axiovision software by Zeiss
Sourced in Germany, United States, United Kingdom, Japan, France, Canada, Italy, Austria, Sweden, Switzerland
AxioVision is a software package developed by ZEISS. It provides a user interface for the control and operation of ZEISS microscopes and imaging systems. The software enables the capture, processing, and analysis of digital images and data generated by ZEISS hardware.
8
Nis elements advanced research version 4 by Nikon
NIS Elements Advanced Research, Version 4.40 is a software package designed for microscope imaging and analysis. It provides a comprehensive suite of tools for capturing, processing, and analyzing digital images from a variety of microscope types, including widefield, confocal, and high-content imaging systems.
9
Axiocam mrc camera by Zeiss
Sourced in Germany, United States, Switzerland, United Kingdom, Japan
The AxioCam MRc is a digital camera designed for microscopy applications. It features a high-resolution sensor and is capable of capturing detailed images of specimens under a microscope. The core function of the AxioCam MRc is to provide high-quality image capture for a variety of microscopy techniques.
10
Polybead microspheres by Polysciences
Sourced in United States, Germany
Polybead Microspheres are uniform, nonporous polymer microspheres that are available in a range of sizes and surface chemistries. These microspheres are designed for use as standards, markers, and tracers in various applications.

More about "Tetramisole"

Tetramisole, also known as levamisole, is a widely used anthelmintic drug that is effective in treating parasitic infections caused by roundworms and hookworms in both humans and animals.
This medication works by inhibiting the enzyme acetylcholinesterase, which leads to spasmodic paralysis and the expulsion of the parasites.
Researchers can optimize their Tetramisole studies by utilizing advanced tools like PubCompare.ai, an AI-driven platform that helps identify the best protocols and products to enhance reproducibility and accuracy.
PubCompare.ai allows users to easily locate and compare Tetramisole protocols from literature, preprints, and patents, taking their research to new heights.
When conducting Tetramisole-related studies, researchers may also utilize other equipment and software, such as the Axio Imager M2 microscope, Poly-L-lysine, Axioscope, AxioCam camera, AxioVision software, NIS Elements Advanced Research, Version 4.40 software, and the AxioCam MRc camera.
These tools can provide valuable insights and support accurate data collection and analysis.
By leveraging the power of AI-driven protocol comparison and accessing a wide range of related equipment and software, researchers can optimize their Tetramisole studies, enhance reproducibility, and drive their research forward to new discoveries.
Experience the power of AI-driven protocol comparison with PubCompare.ai today.