Log In Sign up
The largest database of trusted experimental protocols
> Chemicals & Drugs > Organic Chemical > Thioglycerol

Thioglycerol

Thioglycerol is a organic compound with the chemical formula HOCH2CH(SH)CH2OH.
It is a viscous, colorless liquid with a mild odor.
Thioglycerol is used as a reducing agent, a plasticizer, and an intermediate in the synthesis of other chemicals.
It has applications in the pharmaceutical, cosmetic, and rubber industries.
Researchers can use the PubCompare.ai platform to locate the best protocols from literature, preprints, and patents involving thioglycerol, and optimize their research protocols to boost reproducibility and streamline their work.

Most cited protocols related to «Thioglycerol»

1
Optimizing Cardiac Differentiation of Pluripotent Stem Cells
Cited 139 times
Both hiPSCs and hESCs (>p20) were split at 1:10 or 1:12 ratios, using EDTA as above and grown for 4 days, at which time they reached ~85% confluence. Medium was changed to CDM3, consisting of RPMI 1640 (11875, Life Technologies), 500 µg/mL Oryza sativa-derived recombinant human albumin (A0237, Sigma-Aldrich, 75 mg/mL stock solution in WFI H2O, stored at −20 °C), and 213 µg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich, 64 mg/mL stock solution in WFI H2O, stored at −20 °C). Medium was changed every other day (48 h). For d0-d2, medium was supplemented with 6 µM CHIR99021 (LC Laboratories). On d2, medium was changed to CDM3 supplemented with 2 µM Wnt-C59 (Selleck Chemicals). Medium was changed on d4 and every other day for CDM3. Contracting cells were noted from d7.
Other additions to cardiac differentiation media tested were 10.7 µg/mL recombinant human transferrin, 14 µg/mL sodium selenite, 1 µg/mL linoleic acid, 1 µg/mL linolenic acid, 2 ng/mL triiodo-l-thyronine, 2 µg/mL L-carnitine, 1 µg/mL D,L-alpha-tocopherol acetate, 100 ng/mL retinol acetate, 1 µg/mL ethanolamine, 20 ng/mL corticosterone, 9 ng/mL progesterone, 47 ng/mL lipoic acid, 100 ng/mL retinol, 1 µg/mL D,L-alpha-tocopherol, 100 ng/mL biotin, 2.5 ug/mL catalase, 2.5 µg/mL glutathione, 2.5 µg/mL superoxide dismutase, 2 µg/mL L-carnitine, 15 µg/mL D(+)-galactose, 16.1 µg/mL putrescine, 450 µM 1-thioglycerol, 55 µM 2-mercaptoethanol, and 64 µg/mL L-ascorbic acid 2-phosphate (all from Sigma-Aldrich).
Basal media assessed were DMEM (catalogue #11965), DMEM/F12 (11330), IMDM (12440), IMDM/F12 (12440/11765), RPMI 1640 (11875), McCoy’s 5A (16600), M199 (with Earle’s Salts, 11150), MEMα (with Earle’s Salts, no nucleosides, 12561), and MEM (with Earle’s Salts, 11095) (all from Life Technologies). RPMI 1640 media assessed were RPMI 1640 with L-glutamine (catalogue number 11875), RPMI 1640 with L-glutamine and HEPES (22400), RPMI 1640 with GlutaMAX (61870), and RPMI with GlutaMAX and HEPES (72400) (all from Life Technologies).
Albumin sources assessed were human serum albumin (A1653, Sigma-Aldrich), Oryza sativa-derived recombinant human albumin (A0237, Sigma-Aldrich), Saccharomyces cerevisiae-derived recombinant Albucult (Novozymes Biopahrma/A6608, Sigma Aldrich), Oryza sativa-derived recombinant Cellastim (Invitria/A9731, Sigma Aldrich), and embryo-grade bovine serum albumin (A3311, Sigma Aldrich).
Wnt inhibitors assessed were IWP-2, IWR-1 (both Sigma-Aldrich), XAV-939, ICG-001 (Selleck Chemicals), IWP-4 (Stemgent), and Wnt-C59 (Selleck Chemicals). GSK3B inhibitors assessed were CHIR99021 (LC Laboratories), BIO, TWS119 (Selleck Chemicals), 1-azenkenpaullone, TDZD-8, ARA014418, and 3F8 (all Sigma-Aldrich). Inhibitors used for pathway analysis were PD173074, SB203580, LDN193189, SB431542 (all Selleck Chemicals), SU5402, Dorsomorphin, A83-01 (all Tocris), ALK5 inhibitor (Stemgent), and ITD-1 (Xcessbio). All small molecules were resuspended to 10 mM in dimethyl sulfoxide (DMSO) and used at 5 µM except for Wnt-C59 which was used at 2 µM.
For control treatments (0 µM) 0.1% DMSO was used. Comparisons of differentiation media were made to RPMI+B27-ins consisting of RPMI 1640 (11875) supplemented with 2% B27 without insulin (0050129SA, Life Technologies), and StemPro-34 (Life Technologies) supplemented as shown in Supplementary Table 1. LI-APEL low insulin medium and Xeno-free Differentiation Medium were made as described2 (link), 9 (link), 48 (link). For optimization of cardiac differentiation conditions, cells were differentiated in 12-well plates and samples were analyzed at day 15 of differentiation after dissociation with TrypLE Express for 10 min at 37 °C.
Burridge P.W., Matsa E., Shukla P., Lin Z.C., Churko J.M., Ebert A.D., Lan F., Diecke S., Huber B., Mordwinkin N.M., Plews J.R., Abilez O.J., Cui B., Gold J.D, & Wu J.C. (2014). Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes. Nature methods, 11(8), 855-860.
Publication 2014
2
Culturing Lung, Mast, and Fibroblast Cells
Cited 18 times
The human lung epithelial cell line, A549 (ATCC), was cultured in DMEM/F12 (1:1) medium (HyClone laboratories, Inc., Logan, UT), the human mast cell, HMC-1 (Dr. Joseph Butterfield, Mayo Clinic, Rochester, MN, and a kind gift from professor Gunnar Nilsson at Karolinska Institute, Stockholm, Sweden), was cultured in IMDM (Sigma Aldrich, St. Louis, MO) and the mouse fibroblast cell line, NIH3T3 (ATCC), was cultured in DMEM (with Glutamine; HyClone laboratories, Inc.). All media was supplemented with 10% FBS, 100 units/ml penicillin and 100 µg/ml streptomycin (HyClone laboratories, Inc.). Additional, 2 mM l-glutamine (HyClone laboratories, Inc.) and 1.2 mM alpha-thioglycerol (Sigma Aldrich) were added to the HMC-1 cell media. All supplement FBS was depleted of EVs by ultracentrifugation for 18 hours at 120,000×g (Type 45 Ti rotor, 38, 800 rpm, k-factor 178.6, Beckman coulter, Brea, CA) unless it is specifically mentioned as non-depleted. All cells were cultured at 37°C and 5% CO2.
Shelke G.V., Lässer C., Gho Y.S, & Lötvall J. (2014). Importance of exosome depletion protocols to eliminate functional and RNA-containing extracellular vesicles from fetal bovine serum. Journal of Extracellular Vesicles, 3, 10.3402/jev.v3.24783.
Publication 2014
Cell Lines Cells Dietary Supplements Epithelial Cells Fibroblasts Glutamine Homo sapiens Lung Mast Cell Mus NIH 3T3 Cells Penicillins Streptomycin thioglycerol Ultracentrifugation
3
Forced Aggregation Cardiac Differentiation
Cited 11 times
For forced aggregation hEB differentiations, confluent hESC or hiPSC which had
been grown on Geltrex as monolayers for 3 to 13 passages were passaged with
TrypLE Select and seeded at 2.5×106 per T25 flask. After 24 h
growth, cells were treated with TrypLE Select and seeded at 5000 cells per well
in 96-well V-bottom uncoated plates (249952, NUNC Rochester, NY, http://www.nuncbrand.com) in 100 µL per well RPMI+PVA
medium consisting of RPMI Media 1640 (with L-Glutamine), 4 mg
mL−1 polyvinyl alcohol (P8136 Sigma-Aldrich St. Louis MO,
http://www.sigmaaldrich.com), dissolved in RPMI at 4°C for
at least 72 h, mixing by inversion every day, 1% chemically defined lipid
concentrate, 10 µg mL−1 recombinant human insulin (I9278,
Sigma-Aldrich), 400 µM 1-thioglycerol (Sigma-Aldrich), 25 ng
mL−1 human BMP4 and 5 ng mL−1 human FGF2
(both from R&D systems), 1 µM Y-27632 (Stemgent, Cambridge, MA,
http://www.stemgent.com). This medium is not stable and was made
fresh for each experiment. After 48 hours medium was aspirated with a Costar
8-channel aspirator (Corning Life Sciences, Corning, NY, http://www.corning.com) and replaced with RPMI+FBS medium
consisting of RPMI Media 1640, 20% FBS (Characterized, Hyclone), 400
µM 1-thioglycerol. On day 4 media was aspirated and replaced with
RPMI+INS consisting of RPMI, 1% chemically defined lipid
concentrate, 10 µg mL−1 recombinant human insulin, 400
µM 1-thioglycerol and hEB were transferred to 96-well U-bottom tissue
culture treated plates (NUNC). Media was changed on d7 and every 3 days
afterwards. hEB were visually assessed for contraction on d9 using a Nikon
Eclipse Ti microscope (Nikon Instruments, Melvin, NY, http://www.nikoninstruments.com). Images were captured using
NIS-Elements (Nikon). Other factors that were tested include: Germcell human
serum, Benchmark FBS (Gemini, Sacramento, CA, http://www.gembio.com), Growth
factor reduced Matrigel (BD Biosciences), NODAL, activin A, DKK1,
VEGFA165, WNT3A, TDGF1, BMP2, BMP6, TGFB, IGF1, IGF2, Nidogen
(R&D systems), 96-well U-bottom uncoated plates, 96-well F-bottom tissue
culture plates (NUNC), ITS-X, ITS-G, N2 supplement, B27 supplement,
non-essential amino acids, DMEM, IMDM, F12, KO-DMEM, StemPro-34, KnockOut Serum
Replacement, Xeno-free Knockout Serum Replacement, Qualified FBS (all from
Invitrogen), X-VIVO 10 (Lonza), BSA (A3311), human serum albumin (HSA),
recombinant human albumin (rHA), human transferrin, L-ascorbic acid, L-ascorbic
acid-2-phosphate, Stemline II (Sigma-Aldrich), mTeSR1, SFEM, ES-Cult FBS for
Hematopoietic Differentiation (StemCell Technologies), mouse WNT3A, EX-CYTE
(Millipore, Billerica, MA, http://www.millipore.com).
Traditional cardiac differentiations using FBS were performed as previously
described [6] (link).
Briefly, confluent H9 hESC grown as colonies on MEF were treated with
collagenase IV for 5 min at 37°C then washed from the plate using a 5 mL
pipette. Cell clusters were then transferred to Petri dishes in DMEM (with
Glutamine), 20% FBS (Characterized, Hyclone), 1% NEAA, 100
µM 2-mercaptethanol medium for 7 days. hEB were then transferred to
gelatin coated tissue culture plates. Media was changed every three days.
Burridge P.W., Thompson S., Millrod M.A., Weinberg S., Yuan X., Peters A., Mahairaki V., Koliatsos V.E., Tung L, & Zambidis E.T. (2011). A Universal System for Highly Efficient Cardiac Differentiation of Human Induced Pluripotent Stem Cells That Eliminates Interline Variability. PLoS ONE, 6(4), e18293.
Full text: Click here
Publication 2011
4
Comparative Trypanosomatid Growth Inhibition
Cited 11 times
L. major promastigotes (Friedlin strain; WHO designation MHOM/JL/81/Friedlin), procyclic trypomastigotes of T. brucei brucei S427 29-13 and epimastigotes of T. cruzi CL Brener (genome project standard clone) were adapted for growth in SDM-79 medium supplemented with 10% fetal bovine serum (Gibco, Paisley, UK) and haemin (100 mg·L−1). L. major promastigotes were grown at 24 °C with shaking, and T. brucei and T. cruzi were cultured at 28 °C. T. brucei bloodstream forms were cultured at 37 °C in modified HMI9 medium (56 μm 1-thioglycerol was substituted for 200 μm 2-mercaptoethanol) supplemented with 2.5 μg·mL−1 G418 to maintain expression of T7 RNA polymerase and the tetracycline repressor protein [34 (link)].
In order to directly compare the effects of methylglyoxal on the growth of these trypanosomatids, triplicate cultures containing methylglyoxal were seeded at 5 × 105 parasites per mL. As methylglyoxal interferes with the Alamar blue assay for viable cells, cell densities were determined using the CASY Model TT cell counter (Schärfe, Renlingen, Germany) after culture for 72 h. Concentrations of inhibitor causing a 50% reduction in growth (EC50) were determined using the following two-parameter equation by nonlinear regression using grafit: where the experimental data were corrected for background cell density and expressed as percentages of the uninhibited control cell density. In this equation, [I] represents inhibitor concentration, and m is the slope factor.
Greig N., Wyllie S., Patterson S, & Fairlamb A.H. (2009). A comparative study of methylglyoxal metabolism in trypanosomatids. The Febs Journal, 276(2), 376-386.
Full text: Click here
Publication 2009
2-Mercaptoethanol Alamar Blue antibiotic G 418 Biological Assay Blood Circulation Cells Chagas Disease Clone Cells Culture Media Fetal Bovine Serum Genome Hemin Parasites Proteins Pyruvaldehyde Strains Tetracycline thioglycerol Transcription, Genetic
5
Chemically Defined Fibroblast Reprogramming Protocol
Cited 9 times
Protocols were approved by the Stanford University Human Subjects Research Institutional Review Board. With informed written consent, two 2 mm skin punch biopsies were taken from each volunteer, diced with a scalpel, digested with 1 mg/mL collagenase IV (Life Technologies) for 2 h at 37 °C. Fibroblasts were then grown in DMEM with GlutaMAX (Life Technologies) supplemented with 10% fetal bovine serum (FBS, US origin, Life Technologies) on 6-well plates (Greiner) coated with a 1:200 dilution of growth-factor reduced Matrigel (9 µg/cm2, Corning). Medium was changed every other day. When confluent, fibroblasts were passaged with TrypLE Express (Life Technologies) onto Matrigel-coated T225 flasks (Nunc).
For Sendai reprogramming (Supplemental Fig. 1a), early passage (p2-p3) fibroblasts were seeded at 40,000 cells per well on Synthemax II-SC18 (625 ng/cm2, Corning)-coated 6-well plates. After 24 h, medium was changed to E841 (link). The E8 formula was modified to replace human-derived transferrin with an Oryza sativa-derived recombinant version, to make the formula completely chemically defined. The successful application of Synthemax II-SC at the low concentration of 625 ng/cm2 is in line with reports42 (link) that the minimal surface density of vitronectin protein is very low at 250 ng/cm2. E8 medium consisting of DMEM/F12 (10-092-CM, Corning), 20 µg/mL E. coli-derived recombinant human insulin (Dance Pharmaceuticals/CS Bio), 64 µg/mL L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich), 10.7 µg/mL Oryza sativa-derived recombinant human transferrin (Optiferrin, Invitria/Sigma-Aldrich), 14 ng/mL sodium selenite (Sigma-Aldrich), 100 ng/mL recombinant human FGF2 (154 amino acid, E. coli-derived, Peprotech), and 2 ng/mL recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech). To the medium was added four OSKM CytoTune-iPS Sendai Reprogramming Kit viral particle factors (Life Technologies)43 diluted ~1/5 based on manufacturer’s recommendations (3 × 105 cell infectious units (CIU) of each particle per well, multiplicity of infection (MOI) = 7.5). Medium was changed after 24 h and, thereafter once every day. For the first 7 days, cultures were maintained in E8 supplemented with 100 nM hydrocortisone (Sigma-Aldrich) and 200 µM sodium butyrate (Sigma-Aldrich)44 (link). At day 7 medium was swapped to E7N (E8 minus TGFβ1; supplemented with 200 µM sodium butyrate). Medium switched to E8 at day 20.
For plasmid-based reprogramming45 (link), pCXLE-hSK (27078), pCXLE-hUL (27080), and pCXLE-hOCT4-shp53 (27077) plasmids were obtained from Addgene. The OSKM codon optimized mini-intronic plasmid (CoMiP) was generated by S. Diecke. Plasmid-containing E. coli were grown in Miller’s LB (Life Technologies), and purified using Plasmid Maxi Kit (QIAGEN) following manufacturer’s instructions, and quantified using a NanoDrop 2000 (Thermo Scientific). 1 × 106 cells were electroporated with 6 µg total DNA (2 µg of each for 3 plasmid-based systems, 6 µg for CoMiP) using a Neon Transfection System (Life Technologies), with the settings: 1650 V, 3 pulses, 10 ms, and 100 µL tips, and Buffers R and E2. Cells were plated on Synthemax II-SC-coated 6-well plates.
For peripheral blood mononuclear cell (PBMC) reprogramming, 20 mL of blood was collected in EDTA-containing Vacutainer tubes (BD Biosciences). PBMCs were isolated using a Ficoll-Paque PLUS (1.077 g/mL) gradient (GE Healthcare) and plated at 1 million cells per mL in 2 mL of a humanized version of blood medium46 (link) comprised of 50:50 IMDM:F12 (both Life Technologies), 2 mg/mL recombinant human albumin, 1% v/v chemically defined lipid concentrate (Life Technologies), 10 µg/mL recombinant human insulin, 100 µg/mL recombinant human transferrin, 15 ng/mL sodium selenite, 64 µg/mL L-ascorbic acid 2-phosphate, 450 µM 1-thioglycerol (Sigma-Aldrich), 50 ng/mL SCF (Peprotech), 10 ng/mL IL3 (Peprotech), 2 U/mL EPO (EMD Millipore), 40 ng/mL IGF1 (Peprotech), and 1 µM dexamethasone (Sigma-Aldrich). Cells were cultured for 9 days with 50% medium changes every other day. After 9 days, 1 × 106 were plated in blood medium with Sendai virus, as above. Medium was changed every other day and were transferred to E7N in a Synthemax II-SC-coated 6-well plate at d3.
For all reprogramming methods, individual colonies with hESC morphology were picked into 1 well of a 12-well plate (1 colony per well) at d17-d25 in E8 with 2 µM thiazovivin for 24 h after picking. Subsequently, cells were expanded into 6-well plates by passaging 1:1, 1:4, 1:6, 1:8, and finally 1:12, using 0.5 mM EDTA (Life Technologies) in D-PBS without CaCl2 or MgCl2 (Life Technologies) for 7 min at RT.
Burridge P.W., Matsa E., Shukla P., Lin Z.C., Churko J.M., Ebert A.D., Lan F., Diecke S., Huber B., Mordwinkin N.M., Plews J.R., Abilez O.J., Cui B., Gold J.D, & Wu J.C. (2014). Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes. Nature methods, 11(8), 855-860.
Publication 2014

Most recents protocols related to «Thioglycerol»

1
Stabilizing Bendamustine in PEG-Propylene Glycol Solutions
Not available on PMC !

Example 4

Bendamustine-containing compositions were prepared by dissolving bendamustine HCl to a concentration of 50 mg/ml in 90% polyethylene glycol 400 and 10% propylene glycol. 5 mg/ml of thioglycerol, α-lipoic acid or dihydrolipoic acid was added as a stabilizing antioxidant as indicated in Table 4 below. The samples were maintained at 40° C. and analyzed after 15 days or one month for drug content and impurity profile as indicated in Table 4 below. The results obtained are presented in Table 4.

TABLE 4
Stability of Bendamustine (50 mg/ml) in 90% PEG 400,
10% Propylene Glycol and Antioxidant
% Impurities
RRT%
TContent%HP1PG esterTotal
Antioxidant(° C.)Time(mg/mL)Initial0.591.10Imps
Thioglycerol40initial48.8100<LD<LD0
401 month48.599.40.060.200.71
α-lipoic acid40initial49  100<LD<LD0
4015 days48.899.60.190.130.32
401 month48.799.40.340.260.79
Dihydrolipoic40initial49.3100<LD<LD0
acid401 month47.797.40.630.121.84
<LD = Below Level of Detection

As shown in Table 4, bendamustine, when dissolved in a pharmaceutically acceptable fluid, such as a combination of polyethylene glycol and propylene glycol, in the presence of a stabilizing amount of an antioxidant, such as thioglycerol, α-lipoic acid or dihydrolipoic acid, had substantially no increase in total degradants after a period of 1 month. This data supports the position that bendamustine-containing compositions according to the invention have a shelf life of at least about 2 years when stored at temperatures between 5° C. and 25° C.

US11872214B2. Formulations of Bendamustine (2024-01-16). Eagle Pharmaceuticals, Inc. [US]. Inventors: Nagesh R. Palepu [US], Philip Christopher Buxton [GB].
Full text: Click here
Patent 2024
2
Compound Preparation for Cell Culture
We used 4OHT (H7904, Sigma) and doxycycline (D3447, Sigma) as described20 (link). Etoposide (E1383, Sigma) was dissolved in 50 mM DMSO, aliquoted and stored at −20 °C. Before use, Etoposide was added to fresh medium at a final concentration of 20 µM. Rapamycin (1292, Tocris) was dissolved in 100% ethanol at a 27.3 mM concentration, aliquoted and stored at −20 °C. Before use Rapamycin was added to fresh medium from an intermediate 27.3 μM solution at a final concentration of 20 nM. DMOG (D3695, Sigma) was dissolved in water at a 150 mM concentration, aliquoted and stored at −20 °C. Before use, DMOG was added to fresh medium at a final concentration of 1 mM. 1-Thioglycerol (M1753, Sigma) was stored at 4 °C. Before use, 1-Thioglycerol was added to fresh medium at a final concentration of 1 mM. Nutlin-3 (S1061, Selleckchem) was dissolved in DMSO at a 10 mM concentration, aliquoted and stored at −80 °C. Before use, Nutlin-3 was added to fresh medium at a final concentration of 10 μM.
Tighanimine K., Nabuco Leva Ferreira Freitas J.A., Nemazanyy I., Bankolé A., Benarroch-Popivker D., Brodesser S., Doré G., Robinson L., Benit P., Ladraa S., Saada Y.B., Friguet B., Bertolino P., Bernard D., Canaud G., Rustin P., Gilson E., Bischof O., Fumagalli S, & Pende M. (2024). A homoeostatic switch causing glycerol-3-phosphate and phosphoethanolamine accumulation triggers senescence by rewiring lipid metabolism. Nature Metabolism, 6(2), 323-342.
Full text: Click here
Publication 2024
3
Purification of phosphorylated CDK2/CCNE1 complex
The CDK2/CCNE1 complex for crystallography was generated as previously described13 (link) with modifications. Briefly, the cDNAs encoding human full-length CDK2 and residues 96–378 of human CCNE1 with an N-terminal His tag were synthesized and cloned into modified pFastBack1 vectors, and then co-expressed in sf21 cells at a ratio of 1:1. Harvested cells were resuspended in lysis buffer (40 mM HEPES, pH 7.5, 150 mM NaCl, 0.01% 1-thioglycerol, and 25 mM imidazole), and lysates were cleared by centrifugation. The complex was bound on Probond (Invitrogen) resin followed by lysis buffer wash, elution at 250 mM imidazole, TEV protease digestion and overnight dialysis. The protein was again flowed over Probond resin to remove cleaved His tags, and His-tagged TEV. The complex was then subjected to size exclusion chromatography using a HiLoad 26/600 Superdex 200 pg column equilibrated in 40 mM HEPES, pH7.5, 150 mM NaCl, 0.01% 1-thioglycerol. Intact mass analysis indicated the complex was >95% phosphorylated as isolated and required no additional activation.
Zhang Y., Liu Z., Hirschi M., Brodsky O., Johnson E., Won S.J., Nagata A., Petroski M.D., Majmudar J.D., Niessen S., VanArsdale T., Gilbert A.M., Hayward M.M., Stewart A.E., Nager A.R., Melillo B, & Cravatt B. (2024). Expanding the ligandable proteome by paralog hopping with covalent probes. bioRxiv.
Publication Preprint 2024
4
Tissue Cryoprotection and 3D Imaging
Tissues were washed in 1X DPBS followed by cryoprotection in 30% sucrose for 24 hours and subsequently frozen in OCT (Thermo Scientific) on dry ice and stored at -80°C degrees. Coronal sections of 200μm were prepared from frozen tissue OCT blocks using a cryostat (Leica). Sections were washed in 1X DPBS and then permeabilized and blocked by incubating in block/perm buffer (DPBS/0.3% Triton X-100/1% BSA/1% normal mouse serum) overnight at 37°C degrees. After, samples were incubated with primary antibodies diluted in block/perm buffer at 37°C degrees for 3-4 days. Next, samples were washed in DPBS/0.3% Triton X-100/0.5% 1-thioglycerol for 1-2 days at room temperature, then incubated with secondary antibodies diluted in block/perm buffer at 37°C degrees for 3-4 days. Samples were washed in DPBS/0.3% Triton X-100/0.5% 1-thioglycerol for 1-2 day and then cleared by soaking in Ce3D clearing solution (DPBS/22% [weight/volume] N-methylacetamide/86% [weight/volume] Histodenz/0.7% Triton X-100) overnight. Samples were then mounted in fresh clearing solution and imaged. All preparations were scanned using a Nikon A1R laser scanning confocal including 405, 488, 561, and 650 laser lines for excitation and imaging with 16X/0.8 NA Plan Apo long working distance water immersion objective. Z steps were acquired every 3μm.
Kumar R., Kumar S., Mickael C., Fonseca Balladares D., Nolan K., Lee M.H., Sanders L., Nilsson J., Molofsky A.B., Tuder R.M., Stenmark K.R, & Graham B.B. (2024). Interstitial macrophage phenotypes in Schistosoma-induced pulmonary hypertension. Frontiers in Immunology, 15, 1372957.
Full text: Click here
Publication 2024
5
Cell culture protocol for viability
Conventional culture dishes that are 50 mm in diameter were purchased from Falcon (Sigma-Aldrich, Milan, Italy). Leibovitz’s L-15 medium, α-MEM GlutaMAX medium, insulin transferrin selenium (ITS) 100×, and LIVE/DEAD Fixable Far Red stain were purchased from Invitrogen (Milan, Italy). Penicillin-streptomycin 100×, amphotericin B 250 μg/mL, bovine serum albumin, L-ascorbic acid, L-glutamine 200 mM, Hoechst 33342, fructose, and α-thioglycerol were purchased from Sigma-Aldrich (Milan, Italy). Mayers’s hematoxylin and paraffin wax were purchased from Carlo Erba (Milan, Italy).
Fragomeni G., De Napoli L., De Gregorio V., Genovese V., Barbato V., Serratore G., Morrone G., Travaglione A., Candela A., Gualtieri R., Talevi R, & Catapano G. (2024). Enhanced solute transport and steady mechanical stimulation in a novel dynamic perifusion bioreactor increase the efficiency of the in vitro culture of ovarian cortical tissue strips. Frontiers in Bioengineering and Biotechnology, 12, 1310696.
Full text: Click here
Publication 2024

Top products related to «Thioglycerol»

1
1 thioglycerol by Merck Group
Sourced in United States, Japan, Germany, United Kingdom
1-thioglycerol is a colorless, viscous liquid chemical compound. It is commonly used as a laboratory reagent in various applications, including the synthesis of organic compounds and the study of biochemical processes. The core function of 1-thioglycerol is to serve as a versatile building block and intermediate in chemical reactions.
2
α thioglycerol by Merck Group
Sourced in United States, Italy
α-thioglycerol is a chemical compound used in various laboratory applications. It serves as a reducing agent and can be utilized in synthetic organic chemistry reactions. The core function of α-thioglycerol is to provide a source of thiol groups for chemical transformations.
3
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
5
Glutamax by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Switzerland, Canada, Japan, Australia, China, Belgium, Italy, Denmark, Spain, Austria, Netherlands, Sweden, Ireland, New Zealand, Israel, Gabon, India, Poland, Argentina, Macao, Finland, Hungary, Brazil, Slovenia, Sao Tome and Principe, Singapore, Holy See (Vatican City State)
GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
6
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
7
Ascorbic acid by Merck Group
Sourced in United States, Germany, United Kingdom, France, Italy, India, China, Sao Tome and Principe, Canada, Spain, Macao, Australia, Japan, Portugal, Hungary, Brazil, Singapore, Switzerland, Poland, Belgium, Ireland, Austria, Mexico, Israel, Sweden, Indonesia, Chile, Saudi Arabia, New Zealand, Gabon, Czechia, Malaysia
Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
8
Iscove modified dulbecco media (imdm) by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Germany, Canada, France, Italy, Austria, Israel, Switzerland, Belgium, Australia
IMDM (Iscove's Modified Dulbecco's Medium) is a cell culture medium formulated for the growth and maintenance of a wide variety of cell types, including hematopoietic cells. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.
9
Non essential amino acids (neaa) by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Canada, France, Japan, China, Australia, Switzerland, Belgium, Italy, Austria, Sweden, Ireland, Spain, Gabon, Brazil, Netherlands, Holy See (Vatican City State), Israel, Argentina, Colombia, Sao Tome and Principe
Non-essential amino acids are a group of amino acids that can be synthesized by the human body and are not required to be obtained through diet. These amino acids play a fundamental role in various biological processes, including protein synthesis and cellular function.
10
Bovine serum albumin (bsa) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

More about "Thioglycerol"

Thioglycerol, also known as 1-thioglycerol or α-thioglycerol, is a versatile organic compound with the chemical formula HOCH2CH(SH)CH2OH.
It is a viscous, colorless liquid with a mild odor, and has a variety of applications in the pharmaceutical, cosmetic, and rubber industries.
One of the key uses of thioglycerol is as a reducing agent, where it can help facilitate chemical reactions by providing electrons.
It is also used as a plasticizer, helping to improve the flexibility and durability of materials.
Importantly, thioglycerol serves as an intermediate in the synthesis of other chemicals, making it a valuable starting point for the production of a wide range of compounds.
In the pharmaceutical and cosmetic fields, thioglycerol may be combined with other ingredients like Penicillin/streptomycin, L-glutamine, GlutaMAX, FBS, Ascorbic acid, IMDM, and Non-essential amino acids to create specialized formulations.
These formulations can be used for a variety of purposes, such as wound healing, skin care, and cell culture applications.
Researchers can leverage the PubCompare.ai platform to locate the best protocols from literature, preprints, and patents involving thioglycerol and related compounds.
This can help them optimize their research protocols, boost reproducibility, and streamline their work, ultimately leading to more efficient and effective discoveries.
By incorporating the insights gained from PubCompare.ai, researchers can stay at the forefront of the latest developments in thioglycerol-based research and applications.