The antioxidant capacity of the medicinal plants was estimated spectrophotometrically following the procedure of Benzie and Strain[6 (link)]. The method is based on the reduction of Fe3+ TPTZ complex (colorless complex) to Fe2+ -tripyridyltriazine (blue colored complex) formed by the action of electron donating antioxidants at low pH. This reaction is monitored by measuring the change in absorbance at 593 nm. The Ferric reducing antioxidant power (FRAP) reagent was prepared by mixing 300 mM acetate buffer, 10 ml TPTZ in 40 mM HCl and 20 mM FeCl3.6H2O in the proportion of 10:1:1 at 37°. Freshly prepared working FRAP reagent was pipetted using 1-5 ml variable micropipette (3.995 ml) and mixed with 5 μl of the appropriately diluted plant sample and mixed thoroughly. An intense blue color complex was formed when ferric tripyridyl triazine (Fe3+ TPTZ) complex was reduced to ferrous (Fe2+) form and the absorbance at 593 nm was recorded against a reagent blank (3.995 ml FRAP reagent+5 μl distilled water) after 30 min incubation at 37°. All the determinations were performed in triplicates. The calibration curve was prepared by plotting the absorbance at 593 nm versus different concentrations of FeSO4. The concentrations of FeSO4 were in turn plotted against concentration of standard antioxidant trolox. The FRAP values were obtained by comparing the absorbance change in the test mixture with those obtained from increasing concentrations of Fe3+ and expressed as mg of Trolox equivalent per gram of sample.
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Triazines
Triazines
Triazines are a class of heterocyclic organic compounds containing a six-membered ring with three nitrogen atoms.
These versatile molecules have a wide range of applications in fields like pharmaceuticals, agriculture, and materials science.
Explore the latest research on triazines using PubCompare.ai, an AI-driven platform that helps you locate the best protocols from literature, pre-prints, and patents, with seamless comparisons to enhance reproducibility and accuracy.
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These versatile molecules have a wide range of applications in fields like pharmaceuticals, agriculture, and materials science.
Explore the latest research on triazines using PubCompare.ai, an AI-driven platform that helps you locate the best protocols from literature, pre-prints, and patents, with seamless comparisons to enhance reproducibility and accuracy.
Streamline your research process and unlock new inshights with PubCompare.ai.
Most cited protocols related to «Triazines»
Acetate
Antioxidant Activity
Antioxidants
Buffers
Electrons
Plants
Plants, Medicinal
Strains
TEST mixture
Triazines
Trolox C
Each extract was tested for its reducing power by FRAP assay with some modifications [25 (link)]. Briefly, 20 μL of the sample solution in DMSO with the concentration of 1 mg/mL was mixed with 180 μL of freshly prepared FRAP solution, which contains 0.3 M acetate buffer (pH 3.6), 10 mM 2,4,6 tripyridyl-s-triazine (TPTZ) solution in 40 mM HCl, and 20 mM ferric chloride (10:1:1), and kept in room temperature for 5 min. The absorbance was measured at 595 nm by using a multimode detector (Beckman Coulter DTX880, Fullerton, CA, USA). Ferrous sulfate (FeSO4) was used as a standard and the ferric ions reducing power were expressed as equivalent capacity (EC1) which represented the amount of FeSO4 equivalents per mg of the sample. EC1 was calculated using the following equation:
where a is an absorbance of sample solution with the present of FRAP solution and b is an absorbance of sample solution without the present of FRAP solution. All experiments were done in triplicate.
where a is an absorbance of sample solution with the present of FRAP solution and b is an absorbance of sample solution without the present of FRAP solution. All experiments were done in triplicate.
Acetate
Biological Assay
Buffers
ferric chloride
ferrous sulfate
Ions
Sulfoxide, Dimethyl
Triazines
In this study, most of the chemicals, reagents, and standards were analytical grade and purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Gallic acid, L-ascorbic acid, vanillin, hexahydrate aluminium chloride, Folin-Ciocalteu’s phenol reagent, sodium phosphate, iron(III) chloride hexahydrate (Fe[III]Cl3.6H2O), hydrated sodium acetate, hydrochloric acid, sodium carbonate anhydrous, ammonium molybdate, quercetin, catechin, 2,2′-diphenyl-1-picrylhy-drazyl (DPPH), 2,4,6tripyridyl-s-triazine (TPTZ), and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from the Sigma-Aldrich (Castle Hill, NSW, Australia) for the estimation of polyphenols and antioxidant potential. Sulfuric acid (H2SO4) with 98% purity was purchased from RCI Labscan (Rongmuang, Thailand). HPLC standards including gallic acid, p-hydroxybenzoic acid, caftaric acid, caffeic acid, protocatechuic acid, sinapinic acid, chlorogenic acid, syringic acid, ferulic acid, coumaric acid, catechin, quercetin, quercetin-3-galactoside, diosmin, quercetin-3-glucuronide, epicatechin gallate, quercetin-3-glucoside, kaempferol and kaempferol-3-glucoside were produced by Sigma-Aldrich (Castle Hill, NSW, Australia) for quantification proposes. HPLC and LC-MS grade reagents including methanol, ethanol, acetonitrile, formic acid, and glacial acetic acid were purchased from Thermo Fisher Scientific Inc. (Scoresby, VIC, Australia). To perform various in vitro bioactivities and antioxidant assays, 96 well-plates were bought from the Thermo Fisher Scientific (VIC, Australia). Additionally, HPLC vials (1 mL) were procured from the Agilent technologies (VIC, Australia).
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
4-hydroxybenzoic acid
Acetic Acid
acetonitrile
Aluminum Chloride
ammonium molybdate
Antioxidants
Ascorbic Acid
Biological Assay
caffeic acid
caftaric acid
Catechin
Chlorides
Chlorogenic Acid
Coumaric Acids
Diosmin
diphenyl
epicatechin-3-gallate
Ethanol
ferulic acid
folin
formic acid
Gallic Acid
Glucosides
High-Performance Liquid Chromatographies
Hydrochloric acid
hyperoside
Iron
isoquercetin
kaempferol
Methanol
Phenol
Polyphenols
protocatechuic acid
Quercetin
quercetin 3-O-glucuronide
sinapinic acid
Sodium Acetate
sodium carbonate
sodium phosphate
Sulfonic Acids
Sulfuric Acids
syringic acid
Triazines
vanillin
Total organ vascular leakage was assessed by intravascular administration of Evan's blue (Sigma-Aldrich) as described previously (23 (link)). In brief, Evan's blue (0.2 ml of 0.5% in PBS) was injected intravenously into Cd73−/− or littermate control Cd73+/+ animals that were exposed to normobaric hypoxia (8% O2, 92% N2) or room temperature air for 4 h (n = 4–6 animals per condition). After experimental exposure, the animals were killed, and the colon, skeletal muscle (gluteus maximus), kidney, brain, heart, liver, and lungs were harvested. Evan's blue concentrations in organs were quantified after formamide extraction (55°C for 2 h) by measuring absorbances at 610 nm with subtraction of reference absorbances at 450 nm. This protocol was in accordance with National Institutes of Health (NIH) guidelines for use of live animals and was approved by the Institutional Animal Care and Use Committee at Brigham and Women's Hospital.
In subsets of experiments, mice were reconstituted with 5′-NT purified from Crotalus atrox venom (Sigma-Aldrich). Pilot dosing experiments revealed that 5′-NT could be used at concentrations as high as 500 U/kg i.p. without deleterious effects. After administration of 5′-NT, animals were subjected to normoxia or hypoxia, and examined for vascular leakage using Evan's blue as described before.
In other experiments, mice were administered the adenosine A2A receptor antagonist 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol (ZM 241385; Tocris Cookson Inc.; dosage of 1 mg/kg i.p. plus 1 mg/kg s.c.), adenosine A2B receptor antagonist N-(4-cyanophenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)-phenoxy]acetamide (MRS1754; a gift from K. Jacobson, Molecular Recognition Section, NIH, Bethesda, MD; dosage of 1 mg/kg i.p. plus 1 mg/kg s.c.) or the nonmetabolizable adenosine analogue NECA (Sigma-Aldrich; dosage of 0.1 mg/kg i.p. plus 0.1 mg/kg s.c. based on previous work [28 (link)]). After administration of drug, animals were subjected to normoxia or hypoxia, as indicated, and examined for vascular leakage using Evan's blue as described before. For assessment of pulmonary edema, lungs were collected, weighed, and dried by speed-vac. Weight differences before and after drying were used to calculate lung water content.
In subsets of experiments, mice were reconstituted with 5′-NT purified from Crotalus atrox venom (Sigma-Aldrich). Pilot dosing experiments revealed that 5′-NT could be used at concentrations as high as 500 U/kg i.p. without deleterious effects. After administration of 5′-NT, animals were subjected to normoxia or hypoxia, and examined for vascular leakage using Evan's blue as described before.
In other experiments, mice were administered the adenosine A2A receptor antagonist 4-(2-[7-amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol (ZM 241385; Tocris Cookson Inc.; dosage of 1 mg/kg i.p. plus 1 mg/kg s.c.), adenosine A2B receptor antagonist N-(4-cyanophenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)-phenoxy]acetamide (MRS1754; a gift from K. Jacobson, Molecular Recognition Section, NIH, Bethesda, MD; dosage of 1 mg/kg i.p. plus 1 mg/kg s.c.) or the nonmetabolizable adenosine analogue NECA (Sigma-Aldrich; dosage of 0.1 mg/kg i.p. plus 0.1 mg/kg s.c. based on previous work [28 (link)]). After administration of drug, animals were subjected to normoxia or hypoxia, as indicated, and examined for vascular leakage using Evan's blue as described before. For assessment of pulmonary edema, lungs were collected, weighed, and dried by speed-vac. Weight differences before and after drying were used to calculate lung water content.
acetamide
Adenosine
Adenosine-5'-(N-ethylcarboxamide)
Aftercare
Animal Diseases
Animals
Blood Vessel
Brain
Buttocks
Colon
Evans Blue
formamide
Heart
Hypoxia
Institutional Animal Care and Use Committees
Kidney
Liver
Lung
Mice, House
MRS 1754
NT5E protein, human
Phenols
Pulmonary Edema
Purinergic P1 Receptor Antagonists
Rattlesnake Venoms
Skeletal Muscles
Triazines
ZM 241385
The chelating effect on ferrous ions of the prepared extracts was estimated by the method of Dinis with slight modifications [10 (link)]. Briefly, 100 μL of each test sample (1 mg/mL) was taken and raised to 3 mL with methanol. 740 μL of methanol was added to 20 μL of 2 mM FeCl2. The reaction was initiated by the addition of 40 μL of 5 mM ferrozine into the mixture, which was then left at room temperature for 10 min and then the absorbance of the mixture was determined at 562 nm.
(i) Ferric Ion Reducing Antioxidant Power (FRAP Assay). FRAP activity was measured according to the method of Benzie and Strain [11 (link)]. Briefly, acetate buffer (300 mM, pH 3.6), TPTZ (2,4,6-tripyridyl-s-triazine) 10 mM in 40 mM HCl and FeCl3·6H2O (20 mM) were mixed in the ratio of 10 : 1 : 1 to obtain the working FRAP reagent. Test sample (0.5 mL) was mixed with 3 mL of working FRAP reagent and absorbance was measured at 593 nm after vortexing. Methanol solutions of FeSO4·7H2O ranging from 100 to 2000 μM were prepared and used for the preparation of the calibration curve of known Fe2+ concentration. The parameter equivalent concentration was defined as the concentration of antioxidant having a Ferric-TPTZ reducing ability equivalent to that of 1 mM FeSO4·7H2O.
(i) Ferric Ion Reducing Antioxidant Power (FRAP Assay). FRAP activity was measured according to the method of Benzie and Strain [11 (link)]. Briefly, acetate buffer (300 mM, pH 3.6), TPTZ (2,4,6-tripyridyl-s-triazine) 10 mM in 40 mM HCl and FeCl3·6H2O (20 mM) were mixed in the ratio of 10 : 1 : 1 to obtain the working FRAP reagent. Test sample (0.5 mL) was mixed with 3 mL of working FRAP reagent and absorbance was measured at 593 nm after vortexing. Methanol solutions of FeSO4·7H2O ranging from 100 to 2000 μM were prepared and used for the preparation of the calibration curve of known Fe2+ concentration. The parameter equivalent concentration was defined as the concentration of antioxidant having a Ferric-TPTZ reducing ability equivalent to that of 1 mM FeSO4·7H2O.
Acetate
Antioxidants
Biological Assay
Buffers
Ferrozine
Methanol
Strains
Triazines
Most recents protocols related to «Triazines»
Example 7
The title compound was prepared according to general procedure A using Intermediate 2 (15 mg, 0.04 mmol) and 2-amino-2-methyl-1-propanol (4.73 mg, 0.053 mmol). The crude product was purified by reverse-phase preparatory HPLC to give the title compound (2.8 mg, 16%) as a light yellow solid. 1H NMR (400 MHz, CD3OD) δ 1.40 (t, 3H), 1.47 (s, 6H), 3.70 (s, 2H), 4.16 (q, 2H), 7.20 (dd, 1H), 7.53 (dd, 1H), 7.71 (dd, 1H), 8.56-8.58 (m, 1H), 8.61 (d, 1H), 9.32 (s, 1H), 9.48 (d, 1H). MS (ES+) 411.0 (M+H).
1H NMR
2-amino-2-methyl-1-propanol
High-Performance Liquid Chromatographies
Light
Triazines
Example 3
2,4-Dichloro-6-phenyl-1,3,5-triazine (30 g, 132.71 mmol), carbazole (17.75 g, 106.17 mmol), and sodium tert-butoxide (14.03 g, 145.98 mmol) were put in a round-bottomed flask and then, stirred with 650 ml of THF at ambient temperature for 12 hours. A solid generated therein was filtered and then, stirred in an aqueous layer for 30 minutes. After the filtration, the solid was dried to obtain 20 g (42%) of Intermediate Int-14.
Anabolism
carbazole
Filtration
Sodium
TERT protein, human
Triazines
Pristine TC fabric and the DR, DY, DG, or DB singly dyed T/C fabrics from Sect. 2.2.2 were separately soaked in 1 M NaOH over 1 h to deprotonate the hydroxyl groups of the cotton yarn. The alkalized fabric was then placed in a solution of cyanuric chloride (2,4,6-trichloro-1,3,5-triazine; variable concentration based on amount of thionine used in Table 1 ) in tetrahydrofuran and incubated for 12 h at 40 °C, after which deionized water and tetrahydrofuran were used to wash out unreacted cyanuric acid and other impurities. The activated fabric was then submerged in a solution of thionine acetate (TA; 0.25 molar equiv of cyanuric chloride) in 80 mL deionized (DI) water at 40 °C for another 12 h. The conjugated fabrics were first dried at 40 °C, and then washed with deionized (DI) water and phosphate buffered saline (PBS; aqueous solution of 340 mM NaCl, 6.8 mM KCl, 20.0 mM Na2HPO4, 1.8 mM KH2PO4) to remove free thionine acetate. The washings were collected for calculating the amount of TA loaded onto the fabrics as follows: to ensure the successful removal of unbound thionine from the washing procedure, the prepared fabrics were placed into 3 mL PBS/cm2 fabric for 1 h with vigorous shaking before being transferred to a cuvette and the absorbance at 603 nm, corresponding to the absorption maximum of thionine acetate, was recorded. When the leaching of TA could no longer be observed within the detection limit of the spectrometer, the fabrics were then dried at 40 °C in an oven and stored in the dark until further use. Different initial concentrations of TA were employed to explore the concentration-dependent bactericidal activity of TA, and the names and relative the amounts of TA used are listed in Table 1 .
Concentrations of thionine and cyanuric chloride used in the production of the TA-conjugated TC fabrics
| TC-TA% (w.o.f.) | Amount of TA (mmol) | Amount of TCT (mmol) |
|---|---|---|
| 0% (TC) | 0 | 0 |
| 0.72% (TC-TA1) | 0.125 | 0.5 |
| 1.44% (TC-TA2) | 0.25 | 1 |
| 5.76% (TC-TA3) | 1 | 4 |
| 11.52% (TC-TA4) | 2 | 8 |
Acetate
cyanuric acid
cyanuric chloride
Gossypium
GZMB protein, human
Hydroxyl Radical
Molar
Phosphates
Saline Solution
Sodium Chloride
tetrahydrofuran
thionine
Triazines
The monomers used were 1, 3,
5-triazine-2, 4, 6-triamine (melamine, 99%) from Merck, Darmstadt,
Germany, and polyoxymethylene (paraformaldehyde, extra pure) from
BDH, Poole, UK. Acetonitrile (ACN, p.a.), formic acid (FA, 98–100%),
phosphoryl trichloride (POCl3; 99%), α-hydro-ω-hydroxy-poly[oxy(1-methylethylene)]
[PPG4000; poly(propylene oxide), 4000 Da], iodoacetamide (IAA), 1,
4-dithiothreitol (DTT), triethylammonium bicarbonate (TEAB) buffer
(1 M), deuterium oxide (D2O, 99.9% atom-% D), and deuterated
acetonitrile (CD3CN, >99.8 atom-% D) were from Merck.
Trifluoroacetic
acid (TFA) was a product of VWR Chemicals (Radnor, PA, USA). The methanol
used in the Soxhlet extraction was of analytical grade from Prolabo,
obtained from VWR. Tetrahydrofuran (THF, 99.5% anhydrous, < 0.005%
H2O dried over molecular sieves, stabilized with 2, 6-di-tert-butyl-4-methylphenol) was from Scharlau (Barcelona,
Spain). Phenylphosphoric acid (PPA) was a product of TCI (Geel, Belgium).
The amphiphilic triblock copolymers Pluronic L61, L121, P123, and
F127, α, ω-hydroxy-poly(oxyethylene)-block-poly[oxy(1-methylethylene)]-block-poly(oxyethylene)
of varying overall molecular weights and block ratios were gifts from
BASF (Ludwigshafen, Germany). Dialysis tubing (MWCO 1000 Da) was from
Spectrum Laboratories (New Brunswick, NJ, USA). The GADDYYTAR peptides
(non- and mono-phosphorylated on tyrosine) were custom-synthesized
by LifeTein (Somerset, NJ, USA), and trypsin was from Promega (Madison,
WI, USA). All chemicals were used as received, unless otherwise noted.
Water used was prepared by Milli-Q or Ultra-Q equipment from Merck
Millipore (Bedford, MA, USA).
5-triazine-2, 4, 6-triamine (melamine, 99%) from Merck, Darmstadt,
Germany, and polyoxymethylene (paraformaldehyde, extra pure) from
BDH, Poole, UK. Acetonitrile (ACN, p.a.), formic acid (FA, 98–100%),
phosphoryl trichloride (POCl3; 99%), α-hydro-ω-hydroxy-poly[oxy(1-methylethylene)]
[PPG4000; poly(propylene oxide), 4000 Da], iodoacetamide (IAA), 1,
4-dithiothreitol (DTT), triethylammonium bicarbonate (TEAB) buffer
(1 M), deuterium oxide (D2O, 99.9% atom-% D), and deuterated
acetonitrile (CD3CN, >99.8 atom-% D) were from Merck.
Trifluoroacetic
acid (TFA) was a product of VWR Chemicals (Radnor, PA, USA). The methanol
used in the Soxhlet extraction was of analytical grade from Prolabo,
obtained from VWR. Tetrahydrofuran (THF, 99.5% anhydrous, < 0.005%
H2O dried over molecular sieves, stabilized with 2, 6-di-tert-butyl-4-methylphenol) was from Scharlau (Barcelona,
Spain). Phenylphosphoric acid (PPA) was a product of TCI (Geel, Belgium).
The amphiphilic triblock copolymers Pluronic L61, L121, P123, and
F127, α, ω-hydroxy-poly(oxyethylene)-block-poly[oxy(1-methylethylene)]-block-poly(oxyethylene)
of varying overall molecular weights and block ratios were gifts from
BASF (Ludwigshafen, Germany). Dialysis tubing (MWCO 1000 Da) was from
Spectrum Laboratories (New Brunswick, NJ, USA). The GADDYYTAR peptides
(non- and mono-phosphorylated on tyrosine) were custom-synthesized
by LifeTein (Somerset, NJ, USA), and trypsin was from Promega (Madison,
WI, USA). All chemicals were used as received, unless otherwise noted.
Water used was prepared by Milli-Q or Ultra-Q equipment from Merck
Millipore (Bedford, MA, USA).
acetonitrile
Acids
Buffers
Deuterium Oxide
Dialysis
Dithiothreitol
formic acid
Gifts
Hydroxytoluene, Butylated
Iodoacetamide
melamine
paraform
Peptides
pluronic L61
poly(propylene oxide)
Poly A
Promega
tetrahydrofuran
Triazines
triethylammonium bicarbonate
Trypsin
Tyrosine
Mtb-specific IgG was measured using a Luminex isotype assay described by Lu et al., 2019.17 (link) Briefly, we bound Luminex MagPlex® carboxylated beads (Luminex) with Mtb-specific antigens and non-Mtb related antigen using N-Hydroxysuccinimide (NHS)-ester linkages following manufacturer's recommendations. Protein antigens were coupled to Magplex carboxylated beads using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide) according to the manufacturer's recommendations. LAM was modified by 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium prior to conjugation. Multiplexed antigen-coupled beads were incubated with sample (supernatant from the QFT-Plus NIL tube) at a 1:100 dilution in assay buffer (PBS with 0.1% BSA and 0.05% Tween) for 18 hours. IgG was detected using phycoerythrin-conjugated mouse antibodies against human antibody subclasses (Southern Biotech 9040-09). Secondary incubations were performed over 2 hours. Analysis was performed on an iQue Screener PLUS using ForeCyt software (Intellicyt). The relative concentration of IgG was expressed as Median Fluorescence Intensity (MFI) and absolute concentrations of these were not calculated. All assays were performed in duplicates.
Antibodies
Antigens
Biological Assay
Buffers
Carbodiimides
Esters
Fluorescence
Homo sapiens
Immunoglobulin Isotypes
Mice, House
N-hydroxysuccinimide
N-hydroxysulfosuccinimide
Phycoerythrin
Proteins
Technique, Dilution
Triazines
Tweens
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.
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Trolox is a water-soluble vitamin E analog that functions as an antioxidant. It is commonly used in research applications as a reference standard for measuring antioxidant capacity.
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The Folin-Ciocalteu reagent is a colorimetric reagent used for the quantitative determination of phenolic compounds. It is a mixture of phosphomolybdic and phosphotungstic acid complexes that undergo a color change when reduced by phenolic compounds.
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Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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2,4,6-tripyridyl-s-triazine is a chemical compound used in analytical laboratory applications. It functions as a chelating agent, capable of forming stable complexes with various metal ions. The compound's primary role is in spectrophotometric analysis, where it is utilized to determine the concentration of specific metal ions in solution.
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
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Sodium carbonate is a water-soluble inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a pH regulator, water softener, and cleaning agent in various industrial and laboratory applications.