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Tribromoethanol

Tribromoethanol is a sedative and hypnotic agent commonly used in laboratory research.
It is known for its ability to induce a state of anesthesia and relaxation in experimental animals.
PubCompare.ai's innovative protocol comparison tool can help researchers optimize their Tribromoethanol studies by identifying the most accurate and reproducible experimental protocols from the literature, pre-prints, and patents.
This AI-driven platform allows users to easily compare different approaches, select the best products, and improve the quality and consistency of their Tribromoethanol experiments.
Experince the future of research optimization today with PubCompare.ai.

Most cited protocols related to «Tribromoethanol»

Measuring Pulmonary Hypertension in Mice

Cited 18 times

After set duration of hypoxic or normoxic exposure, mice were weighed and anesthetized with Avertin (tribromoethanol) 0.375 mg/g body weight injected intraperitoneally. A tracheostomy was performed with a 22 gauge angiocatheter and secured in place with a 4.0 silk suture. Mice were ventilated with a Harvard Mini-Vent with a stroke volume of 325 µl and rate of 150 stroke/min. Anesthesia was maintained throughout with 1% isoflurane mixed with room air or 10% O₂. After thoracotomy, a 25 gauge needle fitted to a pressure transducer was inserted into the right ventricle. Right ventricular systolic pressure (RVSP) was measured and continuously recorded on a Gould polygraph (model TA-400, Gould instruments, Cleveland, Ohio). Immediately after RVSP measurements were obtained, the mice were sacrificed.
An expanded Materials and Methods section is available in the online data supplement at http://circres.ahajournals.org and provides details of all materials, bone marrow transplantation, pulmonary vascular morphometry, Western blot, immunohistochemistry, immunofluorescence and statistical analyses.

Young K.C., Torres E., Hatzistergos K.E., Hehre D., Suguihara C, & Hare J.M. (2009). INHIBITION OF THE SDF-1/CXCR4 AXIS ATTENUATES NEONATAL HYPOXIA-INDUCED PULMONARY HYPERTENSION. Circulation research, 104(11), 1293-1301.

Publication 2009

Anesthesia Blood Vessel Body Weight Bone Marrow Transplantation Cerebrovascular Accident Dietary Supplements Hypoxia Immunofluorescence Immunohistochemistry Isoflurane Lung Mus Needles Silk Stroke Volume Sutures Systolic Pressure Thoracotomy Tracheostomy Transducers, Pressure tribromoethanol Ventricles, Right Western Blot

Cdkl5 Knockout Mouse Handling

Cited 13 times

All mice were handled according to protocols approved by the Italian Ministry of Health and commensurate with NIH guidelines for the ethical treatment of animals. Mice for testing were produced by crossing Cdkl5^KO/+ females with Cdkl5^KO/Y males and Cdkl5^KO/X females with +/Y male. Littermate controls were used for all experiments. A portion of the behavioral data derived from knockout mice containing the neomycin selection cassette. No difference in behavior was noted between this allele and the neo-negative allele and the results were combined. For dendritic reconstructions mice with sparse fluorescent labeling of cortical neurons were obtained by crossing Thy1::GFP/Thy1::GFP;+/Y males with +/+;Cdkl5^KO/X females and Thy1::GFP/+;Cdkl5^KO/Y males with +/+;Cdkl5^KO/X females. After weaning, mice were housed three to five per cage on a 12 h light/dark cycle (lights off at 19:00 h) in a temperature-controlled environment (21±2 C) with food and water provided ad libitum. For tissues collections all surgery was performed under anesthesia with tribromoethanol 250 mg/Kg (avertin). All efforts were made to minimize suffering.

Amendola E., Zhan Y., Mattucci C., Castroflorio E., Calcagno E., Fuchs C., Lonetti G., Silingardi D., Vyssotski A.L., Farley D., Ciani E., Pizzorusso T., Giustetto M, & Gross C.T. (2014). Mapping Pathological Phenotypes in a Mouse Model of CDKL5 Disorder. PLoS ONE, 9(5), e91613.

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Publication 2014

Alleles Anesthesia Animals Cortex, Cerebral Dendrites Environment, Controlled Females Food Light Males Mice, House Mice, Knockout Neomycin Neurons Operative Surgical Procedures Reconstructive Surgical Procedures tribromoethanol

Establishment of Patient-Derived Pancreatic Tumor Xenografts

Cited 10 times

Our use of the SCID mouse-patient tumor xenograft model has been previously described ([31 (link),33 (link),41 (link),32 (link),34 (link),30 (link)]). For these studies, surgical specimens of patients' pancreatic tumors were received shortly after resection through the Tissue Procurement Facility (TP) of RPCI and cut into 2 mm × 2 mm pieces in tissue culture medium (RPMI 1640) under sterile conditions. SCID mice were then anesthetized by intraperitoneal injection of 0.4–0.5 ml Avertin (2.5 g 2,2,2-tribromoethanol dissolved in 5 ml 2-methyl-butanol/200 ml ddH₂O) and individual tumor pieces were implanted subcutaneously in the abdominal wall of three mice (1^stpassage) and monitored for growth. The mice used in all experiments were 7–8 weeks old CB17 SCID mice with an average weight of 18–20 g. They were kept in sterile cages (4–5 mice per cage) and fed with autoclaved chow and water. Mice were maintained in air-conditioned and light controlled rooms (12 hr cycles). All procedures, injections and tumor measurements were carried out under a laminar flow hood using aseptic precautions. Tumor specimens that grew to a size of 1 cm (8–12 weeks) were retrieved and subsequently passaged into recipient mice (2^ndpassage) and were considered to have successfully engrafted when these tumors grew. Pathological diagnosis of patient specimens and evaluation of engrafted/ passaged tumors was performed in collaboration with a member of the Pathology Department at RPCI.

Hylander B.L., Pitoniak R., Penetrante R.B., Gibbs J.F., Oktay D., Cheng J, & Repasky E.A. (2005). The anti-tumor effect of Apo2L/TRAIL on patient pancreatic adenocarcinomas grown as xenografts in SCID mice. Journal of Translational Medicine, 3, 22.

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Publication 2005

Asepsis Culture Media Diagnosis Heterografts Injections, Intraperitoneal Mus Neoplasms Operative Surgical Procedures Pancreatic Neoplasm Patients SCID Mice Sterility, Reproductive tert-amyl alcohol Tissue Procurement Tissues TNFSF14 protein, human tribromoethanol Wall, Abdominal

Investigating Ultrasonic Vocalizations in Mice Confronted with Intruders

Cited 10 times

We used the following conditions: females were confronted with vivid and anaesthetized female intruders, while males were confronted with both vivid and anaesthetized male and female intruders. Because males were expected to call more than females, we omitted the vivid male intruder condition. Because there was no condition to compare to, we also refrained from incorporating the experiment with anaesthetized males.
At the day of recording, intruder mice (males and females) were divided into two groups: “Vivid intruders” and “anaesthetized intruders”. Intruder mice were anaesthetized with an intraperitoneal (i.p.) injection of 0.25% tribromoethanol (Sigma-Aldrich, Munich, Germany) in the dose 0.125 mg/g of body weight. Vivid intruders were not treated. The estrus cycle phase of the female mice used in our current experiments was not investigated. Ten minutes before initiation of recording both residents and vivid intruder mice were woken up and kept awake by gentle shaking of the home cage in order to achieve a similar arousing level.
For the recording, resident mice (males and females) were first habituated to the room: Mice in their own home cage were placed on the desk in the recording room for 60 seconds. Subsequently, an unfamiliar intruder mouse was placed into the home cage of resident, and the vocalization behavior was recorded for 3 min using AVISOFT RECORDER 4.1. We recorded ultrasonic vocalizations of male and female mice at a sampling frequency of 300 kHz. The microphone (UltraSoundGate CM16) was connected to a preamplifier (UltraSoundGate 116), which was connected to a computer (all sound recording hardware and software was from Avisoft Bioacoustics, Berlin, Germany).

Hammerschmidt K., Radyushkin K., Ehrenreich H, & Fischer J. (2012). The Structure and Usage of Female and Male Mouse Ultrasonic Vocalizations Reveal only Minor Differences. PLoS ONE, 7(7), e41133.

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Publication 2012

Body Weight Estrus Females Males Mice, House Neoplasm Metastasis tribromoethanol Ultrasonics

Quantifying Antigen-Specific Airway CD8 T Cells

Cited 10 times

Memory CD8 T cells, harvested from mice 35–45 days post-infection, were negatively selected from bronchoalveolar lavage (BAL) using Miltenyi CD8α T Cell Isolation Kit II. Influenza NP_366–374/D^b+ tetramer quantification allowed for equal numbers of antigen-specific cells to be i.t. transferred from donor mice to naïve recipient mice. No more than 1.5×10⁵ antigen-specific airway CD8 T_RM cells were transferred per recipient to approximate physiological numbers of airway T_RM cells. Antibodies used for flow cytometry and cell sorting were BioLegend CD62L [MEL-14], CD8α [53–6.7], CXCR3 [CXCR-173]; eBioscience CD11a [M17/4], CD44 [IM7]; and BD Biosciences CD3ε [145-2C11], CD45.2 [104], CD90.2 [53–2.1], IFN-γ [XMG1.2]. Intravital staining was performed immediately before mouse euthanasia and tissue harvest as previously described (15 (link)). Briefly, to identify T cells resident in various tissues, including the lung parenchyma, 1.5µg of fluorophore-conjugated α-CD3ε antibody in 200λ 1× PBS was intravenously injected into the tail vein of mice; five minutes post-injection, mice were euthanized with Avertin (2,2,2-Tribromoethanol - Sigma) and exsanguinated prior to harvest of BAL and other tissues. Staining for intracellular cytokines was performed as previously described following stimulation in the presence of Brefeldin A for the indicated periods of time (25 (link)). To study cell proliferation, mice were given an intraperitoneal bolus of BrdU (0.8mg) at the time of infection and maintained on BrdU drinking water (0.8mg/mL) until harvest. BrdU incorporation was measured using the BrdU Flow kit (BD Biosciences) following tetramer and antibody staining. Samples were run on a BD Biosciences Canto II or LSR II flow cytometer and analyzed with FlowJo software. Sorting was performed on an Influx or Aria II cell sorter (BD Biosciences).

McMaster S.R., Wilson J.J., Wang H, & Kohlmeier J.E. (2015). Airway-Resident Memory CD8 T cells Provide Antigen-Specific Protection against Respiratory Virus Challenge through Rapid IFN-γ Production. Journal of immunology (Baltimore, Md. : 1950), 195(1), 203-209.

Publication 2015

Antibodies Antigens Brefeldin A Bromodeoxyuridine CD3E protein, human CD8 Antigens CD44 protein, human Cell Proliferation Cells CXCR3 protein, human Cytokine Dimercaprol Euthanasia Flow Cytometry Immunoglobulins Infection Influenza Interferon Type II Lung Memory T Cells Mice, House Mus NRG1 protein, human physiology Protoplasm SELL protein, human T-Lymphocyte Tail Tetrameres Thy-1 Antigens Tissue Donors Tissue Harvesting Tissues TRAIL-R 1MAB tribromoethanol Veins

Most recents protocols related to «Tribromoethanol»

Pulmonary Clearance Model in Mice

The pulmonary clearance model in mice was carried out as previously described [42 (link)]. Briefly, five groups (n = 6) of 6–8-week-old female BALB/C mice were infected intranasally by injecting 40 μl of a bacterial suspension (∼5 × 10⁶ CFU) of each of the WT O35E, ΔfghA, ΔfghA/R, ΔadhC-fghA, and ΔadhC-fghA/R into the nostrils under anesthesia using 250 µL of 25 µg/mL 2,2,2-tribromoethanol. Four-and-a-half-hour post-inoculation, mice were sacrificed by an overdose of the anesthesia (750 µL of 25 µg/mL 2,2,2-tribromoethanol), followed by cervical dislocation. The lungs were excised, homogenized, serially diluted, and plated on TSB agar. Plates were incubated for 48 h followed by colony counting.

Othman D., Elhosseiny N.M., Eltayeb W.N, & Attia A.S. (2024). The Moraxella catarrhalis AdhC–FghA system is important for formaldehyde detoxification and protection against pulmonary clearance. Medical Microbiology and Immunology, 213(1), 3.

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Publication 2024

Intranasal OMVs Immunization Against Pneumonia

BALB/c mice were anesthetized with tribromoethanol and intranasally immunized with 30 μL PBS containing 10 μg OMVs once. One week after immunization, mice were anesthetized with an intraperitoneal injection of tribromoethanol and then intranasally infected with 2 × 10⁹ CFU of A. baumannii WHG40137. Bacteria burden in the lungs of mice with bacterial pneumonia was determined 24 h post the sublethal challenge. Gene expressions were also measured using Bronchoalveolar lavage fluid (BALF) specimens from the singly immunized mice, as previously described [27 (link)]. The ^ΔΔCt method was used to calculate the relative gene expressions using the primer sets in Table S1, targeting TNF-α, IL-6, IL-1β, CYBB, S100A8, and mTOR genes, with β-actin as the housekeep gene. The HiScript II One Step qRT-PCR SYBR Green Kit was used to determine the Ct values according to the manufacturer’s instructions (Vazyme, China).

Li C., Xue H., Du X., Nyaruaba R., Yang H, & Wei H. (2024). Outer membrane vesicles generated by an exogenous bacteriophage lysin and protection against Acinetobacter baumannii infection. Journal of Nanobiotechnology, 22, 273.

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Publication 2024

Intranasal Challenge and Therapeutic Evaluation of A. baumannii

Two weeks following the last immunization, mice were anesthetized with an intraperitoneal injection of tribromoethanol and then challenged intranasally with either a sublethal dose of 2 × 10⁹ or an abdominally lethal dose of 2 × 10⁸ colony-forming unit (CFU) of A. baumannii WHG40137. The challenge doses were confirmed by CFU counts of serial tenfold dilutions on LB agar. The survival rates of the mice were monitored daily for 7 days. Additionally, bacterial burdens in the lungs and blood of mice with bacterial pneumonia were determined 24 h post the sublethal challenge.
To test the therapeutic effects of the immunized sera, BALB/c mice were anesthetized with tribromoethanol and intranasally challenged with a sublethal dose of 2 × 10⁹ CFU of A. baumannii WHG40137. After 24 h of the infection, 100 μL of either the immunized serum or the control serum was injected into each mouse via tail vein. The bacteria burden in the lungs of the mice was measured by plating serial dilutions on agar plates.

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Publication 2024

Radiographic Assessment of Tumor-Induced Bone Destruction

We anesthetized rats of each group with tribromoethanol on day 14 following the establishment of the model, and we captured X-ray films of the animals in the supine position to assess the bone destruction resulting from the tumor of the affected limb.

Duan C., Zhu Y., Zhang Z., Wu T., Shen M., Xu J., Gao W., Pan J., Wei L., Su H, & Shi C. (2024). Esketamine inhibits the c-Jun N-terminal kinase pathway in the spinal dorsal horn to relieve bone cancer pain in rats. Molecular Pain, 20, 17448069241239231.

Publication 2024

MPTP-induced Parkinson's Model Tissue Isolation

Not available on PMC !

On day 5 of last MPTP/p injection, mice were anesthetized with tribromoethanol (300 mg/kg, i.p.) 1 h after drugs treatment. The mice were decapitated, and the striatum (ST) and substantia nigra (SN) was isolated and stored at -80°C until use.

Kim J.H., Kim J.S., Ju I.G., Huh E., Choi Y., Lee S., Cho J.Y., Park B.Y, & Oh M.S. (2024). Coadministration of 6-Shogaol and Levodopa Alleviates Parkinson's Disease-Related Pathology in Mice. Biomolecules & therapeutics.

Publication 2024

Top products related to «Tribromoethanol»

Avertin by Merck Group

Sourced in United States, Germany, Japan, Italy, Canada, United Kingdom, Sweden, Sao Tome and Principe, Macao, China

Avertin is a laboratory reagent used as an anesthetic agent in various animal studies and experiments. It is a combination of 2,2,2-tribromoethanol and tert-amyl alcohol. Avertin induces a state of general anesthesia in animals, allowing for safe and controlled procedures to be performed.

Tribromoethanol by Merck Group

Sourced in United States, Germany, China, United Kingdom, Italy

Tribromoethanol is an organic compound commonly used as a sedative and anesthetic agent in veterinary medicine. It serves as a general anesthetic for small laboratory animals. The compound is a crystalline solid that is soluble in water, ethanol, and other organic solvents. Tribromoethanol is widely utilized in research applications involving animal models.

2 2 2 tribromoethanol by Merck Group

Sourced in United States, Germany, Japan, United Kingdom

2,2,2-tribromoethanol is a chemical compound used in research and laboratory settings. It is a colorless, crystalline solid with a boiling point of approximately 203°C. The compound is commonly used as a sedative and anesthetic agent in animal studies.

T48402 by Merck Group

Sourced in United States, Germany

The T48402 is a laboratory equipment product. It is designed to perform core functions related to laboratory processes. Further details on the specific capabilities and intended use of this product are not available at this time.

2 methyl 2 butanol by Merck Group

Sourced in United States, Germany, Italy

2-methyl-2-butanol is a chemical compound used as a laboratory reagent. It is a colorless, volatile liquid with a distinctive odor. The compound is commonly used as a solvent and in the synthesis of other chemical products.

Tert amyl alcohol by Merck Group

Sourced in United States

Tert-amyl alcohol is a colorless, flammable liquid chemical compound used in various industrial and laboratory applications. It serves as a solvent, intermediate, and reagent in various chemical processes. The core function of tert-amyl alcohol is to provide a stable and versatile solvent and reactant for a range of applications.

Avertin 2 2 2 tribromoethanol by Merck Group

Sourced in United States

Avertin, also known as 2,2,2-Tribromoethanol, is a laboratory chemical used as an anesthetic agent for small animals. It induces a state of general anesthesia when administered. Avertin is primarily used in research settings to facilitate procedures and experimentation on small laboratory animals.

C57bl 6j mice by Jackson ImmunoResearch

Sourced in United States, Montenegro, Japan, Canada, United Kingdom, Germany, Macao, Switzerland, China

C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.

Vt1200 by Leica

Sourced in Germany, United States, United Kingdom, Japan, Australia, Canada, Israel, Switzerland

The VT1200S is a vibrating microtome designed for precision sectioning of biological samples. It features a high-precision feed system and a stable base for consistent, uniform sectioning.

Stereotaxic apparatus by Stoelting

Sourced in United States, Ireland, Germany, Israel

A stereotaxic apparatus is a specialized laboratory instrument used to precisely position and orient surgical instruments or experimental tools within a subject's brain or other three-dimensional anatomical structure. It provides a standardized frame of reference to accurately target specific regions of the brain or body for various research and clinical applications.

More about "Tribromoethanol"

Tribromoethanol, also known as Avertin or 2,2,2-tribromoethanol, is a popular sedative and hypnotic agent widely used in laboratory research.
This chemical compound is known for its ability to induce a state of anesthesia and relaxation in experimental animals, making it a valuable tool for researchers.
Tribromoethanol (T48402) is often used as an anesthetic in rodents, such as C57BL/6J mice, during various procedures like stereotaxic surgery, where a VT1200S stereotaxic apparatus may be utilized.
The sedative properties of Tribromoethanol allow researchers to perform delicate experiments with better control and reduced stress on the animal subjects.
PubCompare.ai's innovative protocol comparison tool can help optimize Tribromoethanol studies by identifying the most accurate and reproducible experimental protocols from the literature, pre-prints, and patents.
This AI-driven platform allows users to easily compare different approaches, select the best products, and improve the quality and consistency of their Tribromoethanol experiments.
Additionally, Tert-amyl alcohol, also known as 2-methyl-2-butanol, is a structurally similar compound that has been used as an alternative to Tribromoethanol in some research applications.
Researchers can explore the use of Avertin/ 2,2,2-Tribromoethanol and compare its performance with other anesthetic agents to optimize their experimental designs.
Experince the future of research optimization today with PubCompare.ai and unlock the full potential of your Tribromoethanol studies.