The largest database of trusted experimental protocols

Tricaprylin

Tricaprylin is a medium-chain triglyceride composed of three molecules of caprylic acid (octanoic acid) attached to a glycerol backbone.
It is used in pharmaceutical formulations and as a dietary supplement due to its potential health benefits, including improved fat metabolism and energy production.
Tricaprylin has been studied for its role in supporting cognitive function, weight management, and athletic performance.
However, more research is needed to fully understand its mechanisms of action and optimal therapeutic uses.
PubCompare.ai's AI-driven research protocol optimization can help locate the best scientific protocols and insights on Tricaprylin, ensuring reproducibility and accuracy in your research journry.

Most cited protocols related to «Tricaprylin»

A total of 14 mouse liver samples
were used to prepare a pooled sample. About 60 mg of liver tissue
from each mouse was mixed with deionized water at a ratio of 100 mg/mL.
The mixture was then homogenized for 2 min and stored at −80
°C until use. To extract metabolites from liver tissue, 100 μL
of each homogenized liver sample was mixed with 20 μL of butylated
hydroxytoluene (BHT) mixture (50 mg of BHT into 1 mL methanol) and
800 μL methanol. The mixture was vortexed for 1 min followed
by centrifugation at 4 °C for 10 min at 15 000 rpm. A
portion (700 μL) of the supernatant was aspirated into a plastic
tube and dried by N2 flow. After dissolving the dried sample
with 100 μL of methanol, a stock solution was prepared by diluting
the sample 10 times. Twenty microliter aliquots of each of 14 mouse
liver exacts were combined to make a pooled sample for this work.
A mixture of 16 compound standards was prepared at a concentration
of 100 μg/mL for each compound, including three fatty acid (heptadecanoic
acid, heneicosanoic acid, and nonadecanoic acid), five triglycerides
(trilauroyl-glycerol, trimyristin, tripalmitin, tricaprylin, and tricaprin),
and eight phospholipids (PC(16:0/16:0), PC(16:0/14:0), PC(12:0/12:0),
PC(6:0/6:0), LysoPC(16:0/0:0), LysoPC(10:0), PC(18:2(9Z,12Z)/18:2(9Z,12Z)),
and PC(24:1(15Z)/24:1(15Z)). To prepare the spiked-in samples 100
μL of the pooled sample was added to each of three sample vials,
followed by addition of 20, 50, and 80 μL of the standard mixture
to the first, second, and third vial, respectively. Dichloromethane/methanol
(v/v = 2:1) was then added to each of the three vials to make the
total volume of 200 μL. This resulted in three sample groups
with spiked-in compound standards. The concentration of compound standards
in each of the spiked-in sample groups was 10, 25, and 40 μg/mL,
respectively.
Publication 2014
11-dehydrocorticosterone Centrifugation Fatty Acids Glycerin heneicosanoic acid Liver LYSO-PC Methanol Methylene Chloride Mice, House nonadecanoic acid PC 12 ester Phospholipids Tissues tricaprin tricaprylin Triglycerides trimyristin tripalmitin
Carcinogen-induced primary tumors were induced by administration of 6×1 mg DMBA (DMBA was dissolved in tricaprylin at 5 mg/ml, 0.2 ml/dose, (Sigma, St. Louis, MO); approximately 0.15 µmols/gbw per treatment) by orogastric gavage to female BALB/c mice, once a week starting at 12–14 weeks. This well-established protocol was originally described by Dan Medina, Baylor College, TX, and was used in a prior study by our lab [27] . 20 mice were administered DMBA using this protocol, and 14 mice developed one or multiple mammary gland tumors within 7 months.
The tumors arising in response to a different protocol, but using the same mouse strain and carcinogen, were used as a useful cohort for direct functional and molecular comparison. Thirteen mice were administered DMBA by intraperitoneal injection at 12–14 weeks old (0.01 µmol/gbw, 10× less than the gavage protocol); 10 mice developed mammary gland tumors within 14 months. This protocol induced many types of tumors (principally lung and liver, see [27] ), but more interestingly for this study, induced mammary tumors with a distinct histopathology (not microacinar, and no expression of basal cell associated cytokeratin5).
For serial transplantation experiments, tumors were dissociated, and 5000–10000 single cells were isografted into cleared fat pads as described [28] (link). To test the contribution of host cells to tumor outgrowth, BALB/c tumor cells were transplanted into F1 C57Bl6(EGFP): BALB/c recipients, to ensure immuno-compatibility. Tumor initiating cell frequency was calculated using limdil software (http://bioinf.wehi.edu.au/software/limdil).
Full text: Click here
Publication 2012
9,10-Dimethyl-1,2-benzanthracene Animal Mammary Neoplasms Carcinogens Cells Injections, Intraperitoneal Liver Lung Mice, Inbred BALB C Mus Neoplasms Neoplastic Stem Cells Pad, Fat Strains Transplantation tricaprylin Tube Feeding Woman

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2009
acetonitrile Ammonium ammonium acetate ARID1A protein, human Biological Assay Chromatography, Reverse-Phase diolein Glycerin Lipids Liquid Chromatography Mass Spectrometry Methanol Methylene Chloride monoolein Solvents Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry tricaprin tricaprylin Triglycerides trilaurin trimyristin Triolein tripalmitin
Two animal studies were carried out. In both study, all mice were monitored for their well-being, including body weight (BW), general appearance (e.g., skin, hair [rough hair coat], eyes, nose, breathing, locomotion), clinical signs (e.g., rapid weight loss, diarrhea, bleeding), behavior changes (e.g., eating, drinking, or lethargy), breathing abnormalities, and posture (e.g., hunched posture or body hunching).
In experiment 1, mice were given a single i.p. injection of B(a)P at 100 mg/kg body weight in 0.2 ml of tricaprylin. Six-week-old AJ/p53val135/wt mice were randomized into 6 groups with 18 mice per group (Figure 1A). Gefitinib was freshly prepared in Mazola corn oil before gavage administration. Oral gavage was administered once a day 5 times a week for the duration of the study. Control animals were treated with 0.2 ml corn oil throughout the study. The treatment was begun 2 weeks after the B(a)P injection and continued for 18 consecutive weeks. The daily dose of Gefitinib was 80 mg/kg body weight; oral gavage once a day, 5 days a week. The weekly dose of Gefitinib was 400 mg/kg body weight; oral gavage once weekly. The intermittent dosing of Gefitinib was 400 mg/kg body weight; oral gavage once weekly on weeks 3, 4, 5, 9, 10, 11, 15, 16, 17 with final sacrifice at week 20.
In experiment 2, Swiss/p53val135/wt mice at 8 weeks of age were given the first dose of NTCU and this time point is noted as “week 0”. The dorsal skin of each mouse was shaved 24 to 48 hours prior to the first dose of NTCU. For the application of NTCU, 100-microliter drops of 0.04 M NTCU was applied to the shaved skin with a micro-pipette. This process was repeated twice a week with a 3.5-day interval for 30 consecutive weeks (Figure 2A).
Two weeks after the first dose of NTCU when mice were ∼10 weeks old, mice were divided into the 4 groups and treated with Gefitinib using both daily and weekly dosing regimens. Gefitinib daily (80 mg/kg body weight/day i.g., 5 days a week) and weekly dosing (5 × 80 mg = 400 mg/kg body weight i.g. oral gavage once weekly) were used.
Animals were housed with wood chip bedding in an environmentally controlled, clean-air room with a 12-hour light-dark cycle and a relative humidity of 50%. Drinking water and diet were supplied ad libitum. The study was approved by the Institutional Animal Care and Use Committee at the Medical College of Wisconsin.
For both experiments, body weight was recorded weekly. Mice were euthanized by carbon dioxide (CO2) asphyxiation. Lungs of each mouse were fixed in zinc formalin solution overnight then stored in 70% ethanol [17 (link)]. For the lung AD model, the fixed lungs were evaluated under a dissecting microscope to obtain the surface tumor count and individual tumor diameter. Tumor volume was calculated based on the formula: V = 4πr3/3. The total tumor volume in each mouse was calculated from the sum of all tumors. Tumor load was determined by averaging the total tumor volume of each mouse in each group. For the SCC model, mice were euthanized by CO2 asphyxiation at 32 weeks after the first dose of NTCU treatment. Lungs from all mice were fixed in Zinc formalin overnight then stored in 70% ethanol. Histopathological evaluation of the lung tumors was carried out using the following method [18 (link)]: approximately 100 serial tissue sections (4 μm each) were cut from formalin fixed lung, and one in every 20 sections (approximately 100 μm apart) was stained with hematoxylin and eosin (H&E). The lung SCCs area/lung lobe area ratio was evaluated using NanoZoomer Digital Pathology Virtual Slide Viewer software (Hamamatsu Photonic Co.). H&E-stained slides were scanned with the NanoZoomer HT slide scanner (Hamamatsu Photonics), and virtual slides were analyzed and quantified.
Full text: Click here
Publication 2017
Animals Asphyxia Body Weight Carbon dioxide Congenital Abnormality Corn oil Diarrhea Diet DNA Chips Eosin Ethanol Eye Fingers Formalin Gefitinib Hair Human Body Humidity Institutional Animal Care and Use Committees Lethargy Locomotion Lung Lung Neoplasms Microscopy Mouse, Swiss Mus Neoplasms Nose Skin sodium copper chlorophyllin Tissues Treatment Protocols tricaprylin Tube Feeding Tumor Burden Zinc
Purified DNA samples were size-separated on a 0.7% low melting point (LMP) agarose gel in TAE buffer at 20 V/cm. DNA bands from 20 to 40 kb were excised from the gel and extracted by the phenol method [57 ]. A metagenomic DNA library was constructed using the CopyControl Fosmid Library production kit, according to the manufacturer's protocol (Epicentre Biotechnologies, Madison, WI, USA). For activity screening, the transformed cells were plated onto modified Luria-Bertani (LB) agar plates (5 g/L peptone, 3 g/L yeast extract, 13 g/L bacteriological agar, 10 g/L gum arabic) containing 1% (v/v) emulsified tributyrin as substrate. Cells were grown at 37°C for four days and transformants with clear halos around individual colonies were chosen as possible lipase/esterase producing clones. The selected clones were transferred to 96-well microtiter plates with Terrific Broth and subjected to a second screening for lipolytic activity on the modified LB agar except that 1% (v/v) tricaprylin was used instead of tributyrin. Tricaprylin positive clones were transferred to 96-well microtiter plates and stored. True lipase producing clones were identified amongst the tricaprylin active clones by screening on modified LB agar containing 1% (v/v) triolein. Three clones that showed the strongest lipase activity (FosC12, FosE6 and FosH10) were further analyzed.
Full text: Click here
Publication 2011
Agar Cells Clone Cells DNA Library Esterases Gum Arabic Lipase Lipolysis Metagenome Peptones Phenol Sepharose tributyrin tricaprylin Triolein tris-acetate-EDTA buffer Yeast, Dried

Most recents protocols related to «Tricaprylin»

To study the protein structure at the molecule level, an MD simulation was performed on the GROMAS 2018 software package (http://www.gromacs.org/, accessed on 1 January 2020). The CHARMM36 force field was used for the MD simulation. The crystal structure of lipase NS 40086 (PDB ID: 3TGL) was obtained from the Protein Data Bank as the initial structure in a closed conformation for the simulation. The protein was dissolved in an explicit solvent. Considering that the primary TAGs in the reaction are triolein and tricaprylin, as for the water-free system, 44 triolein molecules (PubChem CID: 5497163) and 47 tricaprylin molecules (PubChem CID: 10850) were added to the simulation box. In the simulation of the PE system, a certain proportion of water molecules (about 9056) was added in the box, the TIP3P water model was selected, and 55 triolein molecules and 39 tricaprylin molecules acted as the oil phase. The model was placed in a cubic box. Energy minimization was performed using the steepest descent method. Furthermore, the system was first equilibrated for 100 ps under an isothermal-isochoric (NVT) system and then for 100 ps under an isothermal-isobaric (NPT) system using a leap-frog integrator and reached the corresponding simulated temperature (298.15 K) and pressure (0.1 MPa). The balanced system was simulated under the NPT system for 5 ns of all-atom molecular dynamics. The LINCS algorithm was used for all the key constraints in the system. Long-range electrostatic interactions were calculated using the Particle Mesh Ewald method with a time step of 2 fs. The root mean square deviation (RMSD) was studied to make sure that the protein structure was fully relaxed. Protein structure characterization and pertinent images were obtained using standard tools supplied by the Gromacs package, PyMol (Schrödinger, Inc., LLC, New York, NY, USA), VMD (University of Illinois, Champaign, IL, USA). The Caver web online server was used to conduct the tunnel analysis of lipase in the water-free system and the PE system [29 (link)].
Full text: Click here
Publication 2024
The production methodology was based on the specialist literature [18, (link)19] (link) and is briefly discussed in this text.
The pilot module comprised a 2 L encased glass reactor, a thermostated bath (TECNAL, TE184), and a mechanical stirrer (TECNAL, TE2003). The pilot modulo was agitated continuously and maintained at 323.15 K. After stabilizing the experimental conditions, the reactor was filled with potassium hydroxide (1 wt%) and alcohol. The reaction was conducted at an oil-alcohol molar ratio of 1:10 (oil-alcohol) for 30 min. After the completion of the reaction, the product was collected and centrifuged to separate the biodiesel-and glycerol-rich phases.
FAEEs and FAMEs were washed repeatedly with distilled water until the pH reached 7.0, with a progressive reduction in washing water volume. Initially, a water-biodiesel ratio of 1:10 at 343.15 K was employed to remove the catalyst, ethanol, and mono-and diacylglycerols. The pH was adjusted by adding sulfuric acid solution at a specific mass ratio of sulfuric acid to biodiesel. After each wash, phase separation was achieved via centrifugation, and the pH of the biodiesel-rich phase was verified. Subsequent washes with sterile water were conducted until a pH of 7.0 was reached. The purified biodiesel was then dried with manganese sulfate (MnSO 4 ) and filtered, and the yield of the transesterification/esterification reaction was determined with gas chromatography to quantify the total esters [18] (link).
A GC-2010/Shimadzu instrument (Shimadzu, San Jose, CA, USA) equipped with a split/splitless injection system was used, operating at 523.15 K, with a split ratio of 100:1, sample injection volume of 1.0 µL, and flame ionization detector (FID) operating at 523.15 K. A polar capillary column ZB-WAX plus/Phenomenex (Torrance, CA, USA) was employed, measuring 30 m in length, 0.32 mm in internal diameter, with a film thickness of 0.25 µm. High-purity hydrogen gas (99.95% LINDE) was used as the carrier gas. The temperature program for the oven and column was as follows: 433.15-498.15 K at 15 K/min, then 433.15-498.15 K at 3 K/min, resulting in a total analysis time of 11 min. The fatty acid composition was determined by identifying fatty acids by comparing retention times with those of standard ester mixtures (tricaprylin). Equation (1) was used to integrate the peak areas via normalization to quantify the fatty acids. The chemical composition of the major fatty acids was determined via gas chromatography (GC) using the European Standard EN14103 [20] (see Table 1).
where m tricaprylin , f tricaprylin , and A tricaprylin represent the mass, response factor, and peak area of the internal standard, respectively; A B is the sum of the peak areas of FAMEs or FAEEs; and m s is the mass of the sample. The average molar masses of sesame FAEEs and FAMEs were calculated based on the fatty acids listed in Table 1, yielding values of 307.738 and 293.714 g/mol for FAEEs and FAMEs, respectively. To estimate the molar mass of the esters formed, we assumed that each fatty acid was completely converted into its respective ester during the transesterification reaction. Based on this assumption, we utilized the methodology described by Halvorsen et al. [21] (link) to estimate the molecular weights of the produced esters.
Publication 2024
All animal experiments (approval number: A19-021-2) were carried out under the Kyushu University Guidelines for Animal Experiments and the ARRIVE guidelines. Eight-week-old female C57BL/6J mice were obtained from Japan SLC (Shizuoka, Japan) and were housed under specific pathogen-free conditions for 1 week before administration of recombinant human SP-D (5 μg in 50 μl of PBS) and BaP (1 mg in 50 μl of tricaprylin) by intratracheal instillation. The mice were sacrificed and analyzed 48 h later. At intratracheal instillation and terminal procedure, the mice were anesthetized with a mixture of medetomidine hydrochloride (0.3 mg/kg), midazolam (4 mg/kg) and butorphanol tartrate (5 mg/kg) intraperitoneally. The mice were monitored for a surgical plane of anesthesia determined by loss of reflexes (lack of response to toe and tail pinch), muscle relaxation, and deep, rhythmic breathing. At termination, the mice were euthanized by exsanguination. The right lung lobes were fixed by intratracheal instillation of 10% neutral-buffered formalin at a pressure of 20 cm H2O and paraffine-embedded for histology, then the left lung lobes were taken for protein extraction.
Full text: Click here
Publication 2024

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2024
Not available on PMC !
After scaled-up synthesis and SPD, TAG species were analyzed by HPLC to further determine which enzyme worked best in producing MLM-type SL TAG species. TAG species determination was performed as described previously with modification 23) (link) . Tricaproin, tricaprylin, tricaprin, trilaurin, triolein and tristearin were used as standards. Their concentrations were at 1 mg/mL after dissolving in dichloromethane. Purified SL was prepared at 5 mg/mL concentration in dichloromethane. Agilent 1260 Infinity Quaternary LC (Agilent Technologies, Inc.) HPLC system equipped with a Sedex Model 85 ELSD (SEDERE SAS Inc., Alfortville, France) was used for TAG species analysis. Separation was achieved with a reverse phase Agilent Zorbax stable bond C18 (250 mm×4.6 mm, 5 µm) column. Column temperature was maintained at 50℃, flow rate at 0.5 mL/ min, injection volume was 5 µL. ELSD drift tube tempera-ture was set at 50℃, nebulizer gas pressure at 3.4 bar and gain at 10. 100% methanol was used as mobile phase A, and mobile phase B was a mixture of acetone/isopropanol (1:1, v/v) . The following gradient system was employed: hold at 1% TAG species were determined based on their equivalent carbon number (ECN) compared with the analyzed standards retention times in the chromatograph, and their relative mol% was calculated based on their peak area.
Publication 2024

Top products related to «Tricaprylin»

Sourced in United States, Germany, Sao Tome and Principe, Denmark, Singapore
Tricaprylin is a synthetic medium-chain triglyceride (MCT) compound. It is a clear, colorless, and odorless liquid. Tricaprylin is primarily used as a laboratory reagent and excipient in pharmaceutical formulations.
Sourced in United States, Germany, France, India, China, United Kingdom
Triolein is a laboratory reagent used as a standard for lipid analysis. It is a triglyceride composed of three oleic acid molecules esterified to a glycerol backbone. Triolein is commonly used as a reference substance in analytical techniques such as gas chromatography and high-performance liquid chromatography to quantify the composition of lipid samples.
Sourced in United States, Germany, United Kingdom
Tributyrin is a laboratory reagent used for various analytical and research applications. It is a triglyceride composed of three butyric acid molecules. Tributyrin serves as a substrate for enzymatic reactions and can be used in the analysis of lipase activity and lipolytic processes.
Sourced in United States
Tripalmitin is a type of lipid molecule that is commonly used in the formulation of various laboratory reagents and products. It is a triglyceride composed of three palmitic acid molecules. Tripalmitin serves as a key component in the preparation of buffers, emulsions, and other laboratory materials where a stable lipid source is required.
Sourced in Germany, United States, United Kingdom, Italy, India, Spain, China, Poland, Switzerland, Australia, France, Canada, Sweden, Japan, Ireland, Brazil, Chile, Macao, Belgium, Sao Tome and Principe, Czechia, Malaysia, Denmark, Portugal, Argentina, Singapore, Israel, Netherlands, Mexico, Pakistan, Finland
Acetone is a colorless, volatile, and flammable liquid. It is a common solvent used in various industrial and laboratory applications. Acetone has a high solvency power, making it useful for dissolving a wide range of organic compounds.
Sourced in United States, Germany, Denmark
Brij 97 is a non-ionic surfactant produced by Merck Group. It is a polyoxyethylene(10) oleyl ether with the molecular formula C26H54O11. Brij 97 is a liquid at room temperature and is commonly used in various laboratory applications.
Sourced in United States, Germany, Italy, China, France, Mexico, Australia, Macao, Japan, New Zealand, Canada, Malaysia, Singapore, Sao Tome and Principe, Poland, Spain, United Kingdom, Chile
α-tocopherol is a chemical compound that functions as a natural antioxidant. It is a type of vitamin E found in various plant oils and other food sources.
Sourced in Germany, United States, United Kingdom, Italy, India, France, China, Australia, Spain, Canada, Switzerland, Japan, Brazil, Poland, Sao Tome and Principe, Singapore, Chile, Malaysia, Belgium, Macao, Mexico, Ireland, Sweden, Indonesia, Pakistan, Romania, Czechia, Denmark, Hungary, Egypt, Israel, Portugal, Taiwan, Province of China, Austria, Thailand
Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
Sourced in United States
The NextSeq 500/550 High Output sequencing reagent Kits v2 are lab equipment used for high-throughput DNA and RNA sequencing. The kits provide the necessary reagents and consumables to perform sequencing runs on the NextSeq 500 or NextSeq 550 sequencing systems.
Sourced in United States, Germany, United Kingdom, India, Italy, China, Poland, France, Spain, Sao Tome and Principe, Canada, Macao, Brazil, Singapore, Ireland, Iceland, Australia, Japan, Switzerland, Israel, Malaysia, Portugal, Mexico, Denmark, Egypt, Czechia, Belgium
Chitosan is a natural biopolymer derived from the exoskeletons of crustaceans, such as shrimp and crabs. It is a versatile material with various applications in the field of laboratory equipment. Chitosan exhibits unique properties, including biocompatibility, biodegradability, and antimicrobial activity. It can be utilized in the development of a wide range of lab equipment, such as filters, membranes, and sorbents, due to its ability to interact with various substances and its potential for customization.

More about "Tricaprylin"

Tricaprylin, a medium-chain triglyceride (MCT), is a compound composed of three molecules of caprylic acid (octanoic acid) attached to a glycerol backbone.
This versatile substance has garnered attention for its potential health benefits, including improved fat metabolism, energy production, and cognitive function.
Tricaprylin's unique composition sets it apart from other triglycerides, such as triolein, tributyrin, and tripalmitin, which have different fatty acid profiles.
The inclusion of caprylic acid, a medium-chain fatty acid, is believed to contribute to Tricaprylin's beneficial effects on the body.
Researchers have explored Tricaprylin's role in weight management, athletic performance, and supporting overall health.
While more studies are needed to fully elucidate its mechanisms of action, the current evidence suggests that Tricaprylin may offer advantages over traditional long-chain triglycerides.
Alongside Tricaprylin, other compounds like acetone, Brij 97, α-tocopherol, and ethanol have been studied for their potential synergistic effects or as part of formulations involving Tricaprylin.
The NextSeq 500/550 High Output sequencing reagent Kits v2 and chitosan have also been utilized in research related to Tricaprylin.
To ensure the accuracy and reproducibility of your research journey, PubCompare.ai's AI-driven protocol optimization can be a valuable tool.
This platform helps researchers locate the best scientific protocols and insights, streamlining the research process and enhancing the quality of your findings.