Experiments were carried out according to National Institutes of Health Guidelines on the Use of Laboratory Animals and all procedures were approved by the Thomas Jefferson University Committee on Animal Care. A total of 497 (384 mice for MI and 113 for I/R) male 8-10 week old C57/B6 mice were used for this study. For the MI model, mice were subjected to permanent coronary artery ligation using either the new (N) method or the classical (C) method. Mice were randomly assigned to four groups: new method of MI (MI-N) or sham (S-N); classical method of MI (MI-C) or sham (S-C). There were 119 mice used for survival study. Some of the mice survived at the end of 28 days were also used for echocardiographic, hemodynamic and infarct size studies as indicated in each study. The rest of 232 mice survived from all kinds of 265 procedures (33 mice died) were used for 24h infarct size measurement (32 mice), Masson's trichrome stain (18 mice), arrhythmia analysis (28 mice), myeloperoxidase (MPO, 81) and TNFα (73) assays. In I/R model, mice were subjected to 30 min of myocardial ischemia followed by 24 hrs of reperfusion. Mice were divided into four groups also: new method of I/R (I/R-N, n=41) or sham (SI/R-N, n=16), classical method of I/R or sham I/R (I/R-C, n=40, SI/R-C, n=16, respectively). All animals were monitored after the surgery and received one dose (0.3mg/kg) of buprenophine within 6 hours post surgery and another dose was administered the following morning. No further analgesia was given thereafter.
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Trichrome stain
Trichrome stain
Trichrome staining is a widely used histological technique that allows for the simultaneous visualization of multiple tissue components.
This staining method utilizes three different dyes to differentially label collagen, muscle, and nuclei, providing a detailed view of tissue architecture.
Trichrome stains are commonly employed in the analysis of various pathological conditions, including fibrosis, inflammation, and neoplastic processes.
By optimizing trichrome stain protocols, researchers can enhance the accuracy and reproducibility of their findings, leading to more reliable and insightful research.
PubCompare.ai's AI-driven platform can assist in this process by effortlessly locating and comparing trichrome stain protocols from literature, preprints, and patents, enabling seamless optimization and improved research outcomes.
This staining method utilizes three different dyes to differentially label collagen, muscle, and nuclei, providing a detailed view of tissue architecture.
Trichrome stains are commonly employed in the analysis of various pathological conditions, including fibrosis, inflammation, and neoplastic processes.
By optimizing trichrome stain protocols, researchers can enhance the accuracy and reproducibility of their findings, leading to more reliable and insightful research.
PubCompare.ai's AI-driven platform can assist in this process by effortlessly locating and comparing trichrome stain protocols from literature, preprints, and patents, enabling seamless optimization and improved research outcomes.
Most cited protocols related to «Trichrome stain»
Animals
Animals, Laboratory
Artery, Coronary
Biological Assay
Buprenorphine
Cardiac Arrhythmia
Echocardiography
Hemodynamics
Infarction
Ligation
Males
Management, Pain
Mice, House
Myocardial Ischemia
Operative Surgical Procedures
Peroxidase
Reperfusion
trichrome stain
Tumor Necrosis Factor-alpha
Representative sampling of the LV (approx. 12 sections) was obtained by transverse sectioning (3 µm) from the apex to the base (atrium region) of paraffin-embedded hearts with an interval of 300 µm among each section (Figure 1A ).
Paraffin sections were stained with modified Masson's trichrome staining (MT). MT staining was performed according to the Trichrome (Masson) Stain kit (Sigma-Aldrich) with the following modifications: nuclei were pre-stained with Celestine Blue solution following staining with Gill's Hematoxylin and incubation for 1 hour in aqueous Bouin's solution to promote a uniform staining.
Paraffin sections were stained with modified Masson's trichrome staining (MT). MT staining was performed according to the Trichrome (Masson) Stain kit (Sigma-Aldrich) with the following modifications: nuclei were pre-stained with Celestine Blue solution following staining with Gill's Hematoxylin and incubation for 1 hour in aqueous Bouin's solution to promote a uniform staining.
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Bouin's solution
Celestine Blue
Cell Nucleus
Heart
Heart Atrium
Hematoxylin
Paraffin
Paraffin Embedding
Biopsy
Ethics Committees, Research
Fibrosis
Formalin
Hematologic Tests
Homo sapiens
Inflammation
Life Experiences
Liver
Non-alcoholic Fatty Liver Disease
Nonalcoholic Steatohepatitis
Pathologists
Patients
Radiologist
Steatohepatitis
Tissue Fixation
trichrome stain
Ultrasonography
Most recents protocols related to «Trichrome stain»
Masson’s trichrome stain kit (G1340, Beijing Solarbio Science and Technology, Beijing, China) was used to stain the prepared paraffin sections (G1340, Beijing Solarbio Science and Technology, Beijing, China). The Masson’s trichrome stain slices were observed by the optical microscope (Leica CTR6, Leica, Germany). ImageJ software (version 1.8.0) was used to quantify the collagen proportion of stained pieces.
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The fractured femur and calluses were collected on day 28 days after the bone fracture. After undergoing decalci cation in 10% formic acid for a duration of one week, the samples were subsequently encased in para n wax. They were then cut longitudinally into sections with a thickness of 5 micrometers and carefully placed onto glass microscope slides. For the H&E staining of bone tissue, sections are rst dewaxed in xylene and rehydrated through a graded alcohol series before being stained with hematoxylin to label nuclei. After rinsing, eosin is applied to stain cytoplasmic elements. In Masson's Trichrome staining, similarly prepared sections undergo sequential staining with Weigert's hematoxylin for nuclear de nition, followed by Biebrich scarlet-acid fuchsin solution, and then differentiated in phosphomolybdic/phosphotungstic acid solution. Collagen is stained blue with aniline blue, providing contrast to red muscle bers. The stained sections are dehydrated, cleared, and mounted. The slides were examined and photographed under an BX53 microscope light microscope (Olympus, Tokyo, Japan).
The slides were re-fixed in Bouin’s solution for 24 h and rinsed with tap water. Tissue sections were stained with Weigert’s iron hematoxylin for 10 min, followed by Biebrich scarlet-acid fuchsin for 15 min. Differentiation was achieved using phosphomolybdic-phosphotungstic acid solution for 10 min. Finally, sections were stained with aniline blue solution for 3 min and washed with water. Stained sections were mounted with xylene-based DPX mountant and visualized using BX60 light microscopy. The MT staining differentiates various cellular components within the kidney tissue. In stained sections, renal tubules appear red, while collagen fibers are stained blue.
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Microwave procedure for Masson’s trichrome kit (HT15-1KT, Sigma-Aldrich, Germany) was used for infarct size estimation with the omission of counter staining with hematoxylin. Skin was the positive control.
5μm slides were prepared from paraffin blocks and mounted on Poly L-lysine coated slides. These slides were dewaxed in xylene and hydrated with decreasing gradations of alcohol and placed in a Coplin jar filled with Bouin’s solution (500ml distilled water was saturated with picric acid and then filtered 167ml formaldehyde and 33.3ml glacial acetic acid were then added to it). This was microwaved for 15seconds followed by a 5mins incubation in fume hood. Slides were then washed with tap water until yellow color on the slides disappeared. Slides were then stained with Biebrich Scarlet-Acid Fuchsin and microwaved for 12seconds, followed by a two minute incubation and then placed in Phosphotungstic/Phosphomolybdic acid (10ml Phosphotungstic acid and 10ml Phosphomolybdic acid is mixed in 20ml deionized water) and microwaved for 12seconds followed by 5mins incubation. Slides were then placed in Analine Blue solution, microwaved for nine seconds, followed by a one minute incubation. After several washes of deionized water, slides were placed in 1% acetic acid (1N acetic acid) for 45 seconds and washed with deionized water for two mins. This was followed by sequential dehydration with alcohol and clearing with two changes of xylene. Slides were then mounted with DPX.
5μm slides were prepared from paraffin blocks and mounted on Poly L-lysine coated slides. These slides were dewaxed in xylene and hydrated with decreasing gradations of alcohol and placed in a Coplin jar filled with Bouin’s solution (500ml distilled water was saturated with picric acid and then filtered 167ml formaldehyde and 33.3ml glacial acetic acid were then added to it). This was microwaved for 15seconds followed by a 5mins incubation in fume hood. Slides were then washed with tap water until yellow color on the slides disappeared. Slides were then stained with Biebrich Scarlet-Acid Fuchsin and microwaved for 12seconds, followed by a two minute incubation and then placed in Phosphotungstic/Phosphomolybdic acid (10ml Phosphotungstic acid and 10ml Phosphomolybdic acid is mixed in 20ml deionized water) and microwaved for 12seconds followed by 5mins incubation. Slides were then placed in Analine Blue solution, microwaved for nine seconds, followed by a one minute incubation. After several washes of deionized water, slides were placed in 1% acetic acid (1N acetic acid) for 45 seconds and washed with deionized water for two mins. This was followed by sequential dehydration with alcohol and clearing with two changes of xylene. Slides were then mounted with DPX.
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Hematoxylin and eosin (H&E) stain, Masson’s trichrome stain, and immunohistochemical (IHC) stain tests were used to study new bone histology and were performed after the harvested calvarial samples were subjected to micro CT scanning. The samples fixed with 10% formalin were again decalcified using ethylene-diamine-tetraacetic acid solution. After demineralization, the samples were embedded in paraffin and sliced into samples with 4 μm thicknesses. Afterward, the slides were stained with hematoxylin and eosin (H&E) and Masson’s trichrome stain. Masson’s trichrome stain was performed in accordance with the manual instruction of Masson’s trichrome stain kit (ab150686, Abcam, Cambridge, UK). The other slides were immunohistochemically stained for type I collagen (NB600-450, Novus Biologicals, Littleton, CO, USA) and osteocalcin (sc-365797, Santa Cruz Biotechnology, Santa Cruz, CA, USA) to evaluate new bone formation characteristics. In order to analyze the trends in collagen type I and osteocalcin protein staining from the IHC-stained images, the stained area relative to the total tissue area, excluding the scaffold area, was calculated using the Image J (Version 1.53q; National Institutes of Health, Bethesda, MD, USA) analysis tool.
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Top products related to «Trichrome stain»
Sourced in China
Masson's Trichrome Stain Kit is a laboratory tool used for the histological staining of tissue samples. It is designed to differentially stain collagen fibers, muscle fibers, and nuclei within the sample.
Sourced in United States, China, United Kingdom
The Trichrome Stain (Masson) Kit is a laboratory product manufactured by Merck Group. It is a staining solution used for the histological examination of tissue samples. The kit provides the necessary reagents to perform the Masson trichrome staining technique, which is commonly used to differentiate between collagen fibers, muscle fibers, and nuclei in various tissue types.
Sourced in United States, Germany, Sao Tome and Principe
Masson's trichrome stain is a histological staining technique used in microscopy to differentiate various tissue components. It primarily stains collagen fibers blue-green, muscle fibers red, and nuclei black. This stain is commonly used in the analysis of connective tissue, muscle tissue, and other biological samples.
Sourced in United Kingdom, United States
The Trichrome Stain Kit is a set of reagents designed for the histological staining of tissue sections. The kit provides the necessary components to perform a trichrome staining procedure, which allows for the differentiation of various cellular and extracellular structures within a sample.
Sourced in United States, Germany
Masson's Trichrome Stain Kit is a laboratory staining product used to differentiate between collagen, muscle, and other connective tissues in histological samples. It provides a standardized set of reagents and a protocol for staining tissue sections.
Sourced in United States, Germany
The Masson's Trichrome Stain Kit is a laboratory product used for staining tissue samples. It is a three-color staining technique that differentiates collagen, muscle, and nuclei in tissue sections.
Sourced in United States, Japan, Germany, United Kingdom, China, Hungary, Singapore, Canada, Switzerland
Image-Pro Plus 6.0 is a comprehensive image analysis software package designed for scientific and industrial applications. It provides a wide range of tools for image capture, enhancement, measurement, analysis, and reporting.
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The BX51 microscope is an optical microscope designed for a variety of laboratory applications. It features a modular design and offers various illumination and observation methods to accommodate different sample types and research needs.
Sourced in United States, Germany, Spain, United Kingdom, China, Canada, Sweden, Italy
Masson's trichrome is a histological staining technique used to differentiate various types of connective tissues within a sample. It stains collagen fibers blue, muscle fibers red, and nuclei black. This stain is commonly used in pathological and research applications to visualize the structure and composition of tissues.
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Image-Pro Plus is an image analysis software developed by Media Cybernetics. It provides tools for image acquisition, processing, measurement, and analysis.