Total RNA was extracted from EBV transformed lymphoblastoid cell line pellets by the TRIzol reagent (Ambion), and mRNA and small RNA sequencing of 465 unique individuals was performed on the Illumina HiSeq2000 platform, with paired-end 75bp mRNA-seq and single-end 36bp small RNA-seq. Five samples were sequenced in replicate in each of the seven sequencing laboratories. The mRNA and small RNA reads were mapped with GEM31 and miraligner32 (link), respectively, with an average of 48.9M mRNA-seq reads and 1.2M miRNA reads per sample after QC. Numerous transcript features were quantified using Gencode v1233 (link) and miRBase v1834 (link) annotations: protein-coding and lincRNA genes (16,084 detected in >50% of samples), transcripts (67,603; with FluxCapacitor7 (link)), exons (146,498), annotated splice junctions (129,805; analyzed in detail in Ferreira et al. submitted), transcribed repetitive elements (47,409), and mature miRNAs (715). Data quality was assessed by sample correlations and read and gene count distributions, and technical variation was removed by PEER normalization35 (link) for the QTL and miRNA-mRNA correlation analyses11 (link). The samples clustered uniformly both before and after normalization. The genotype data was obtained from 1000 Genomes Phase 1 data set for 421 samples (80× average exome and 5× whole genome read depth), and the remaining 41 samples were imputed from Omni 2.5M SNP array data. Furthermore, we did functional reannotation for all the 1000 Genomes variants using Gencode v12. QTL mapping was done with linear regression, using genetic variants with >5% frequency in 1MB window and normalized quantifications transformed to standard normal. Permutations were used to adjust FDR to 5%. Full details are provided in Supplementary Methods .
>
Chemicals & Drugs
>
Organic Chemical
>
Trizol
Trizol
Trizol is a powerful reagent used for the extraction and purification of total RNA from a variety of biological samples.
It is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components that efficiently lyses cells and denatures cellular components to release intact RNA.
Trizol-based protocols are widely used in molecular biology and genomics research for high-yield, high-quality RNA isolation, enabling downstream applications such as RT-PCR, Northern blotting, and RNA-seq.
The ease of use, versatility, and reproducibility of Trizol make it an indispensable tool for researchers seeking to accurately quantify and analyze gene expression profiles across diverse experimental systems.
It is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components that efficiently lyses cells and denatures cellular components to release intact RNA.
Trizol-based protocols are widely used in molecular biology and genomics research for high-yield, high-quality RNA isolation, enabling downstream applications such as RT-PCR, Northern blotting, and RNA-seq.
The ease of use, versatility, and reproducibility of Trizol make it an indispensable tool for researchers seeking to accurately quantify and analyze gene expression profiles across diverse experimental systems.
Most cited protocols related to «Trizol»
Cell Line, Transformed
DNA Replication
Exome
Exons
Genes
Genetic Diversity
Genome
Genotype
Long Intergenic Non-Protein Coding RNA
MicroRNAs
Pellets, Drug
Proteins
Repetitive Region
RNA, Messenger
RNA-Seq
trizol
Thirty-four neuroblastoma cell lines were grown to subconfluency according to standard culture conditions. RNA was isolated using the RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions. Nine RNA samples from pooled normal human tissues (heart, brain, fetal brain, lung, trachea, kidney, mammary gland, small intestine and uterus) were obtained from Clontech. Blood and fibroblast biopsies were obtained from different normal healthy individuals. Thirteen leukocyte samples were isolated from 5 ml fresh blood using Qiagen's erythrocyte lysis buffer. Fibroblast cells from 20 upper-arm skin biopsies were cultured for a short time (3-4 passages) and harvested at subconfluency as described [22 (link)]. Bone marrow samples were obtained from nine patients with no hematological malignancy. Total RNA of leukocyte, fibroblast and bone marrow samples was extracted using Trizol (Invitrogen), according to the manufacturer's instructions.
Arm, Upper
Biopsy
BLOOD
Bone Marrow
Brain
Buffers
Cell Lines
Erythrocytes
Fetus
Fibroblasts
Heart
Hematologic Neoplasms
Homo sapiens
Intestines, Small
Kidney
Leukocytes
Lung
Mammary Gland
Neuroblastoma
Patients
Skin
Tissues
Trachea
trizol
Uterus
Cells
Fetal Bovine Serum
Genome
Mus
Myogenesis
RNA, Messenger
Serum
Skeletal Myocytes
trizol
Mouse skeletal muscle C2C12 cells were initially plated on 15 cm plates in DMEM with 20% fetal bovine serum. At confluence, the cells were switched to low serum medium to initiate myogenic differentiation. For extraction of total RNA, cells were first rinsed in PBS and then lysed in Trizol reagent (Invitrogen catalog # 15596-026) either during exponential growth in high serum medium, or at 60 hrs, 5 days and 7 days after medium shift. Residual contaminating genomic DNA was removed from the total RNA fraction using Turbo DNA-free (Ambion catalog # AM1907M). mRNA was isolated from DNA-free total RNA using the Dynabeads mRNA Purification Kit (Invitrogen catalog # 610-06).
Cells
Fetal Bovine Serum
Genome
Mus
Myogenesis
RNA, Messenger
Serum
Skeletal Myocytes
trizol
Biological Assay
Cells
Complementary RNA
Eukaryotic Cells
Genome, Human
Lipofectamine
MicroRNAs
Oligonucleotides
RNA, Small Interfering
Transfection
trizol
Most recents protocols related to «Trizol»
Homogenized tissue samples (100 mg) were transferred into 1 mL of TRIzol in a 2 mL microcentrifuge tube. A total of 10 µL of fresh BME solution was added, mixed well by vigorous shaking/vortexing, and incubated for 5 min at room temperature. The tube was centrifuged at 12,000 rpm for 10 min at 4 °C. The aqueous phase was transferred into a 1.5 mL microcentrifuge tube and 200 µL of chloroform was added. The sample was mixed well, incubated for 5 min at room temperature, and centrifuged for 10 min at 12,000 rpm for phase separation. The aqueous phase was transferred into a 1.5 mL microcentrifuge tube and 0.7 volume of chilled isopropanol was added. The solution was mixed well by inversion and was incubated for 1 h at −20 °C. The supernatant was decanted after centrifugation at 12,000 rpm for 10 min at 4 °C. The pellet was washed with 70% ethanol. The supernatant was gently poured off or removed using a pipette. This step was repeated. The pellet was air dried and eluted in 50 µL of RNase-free water.
Full text: Click here
The TRIzol-column hybrid method was used to extract RNA from needles, phloem, and root tissues following Untergasser’s protocol [22 ]. First, 500 µL of TRIzol was added to homogenized tissue and was incubated for 5 min at room temperature. Then, 100 µL of chloroform was added and mixed well, and the mixture was incubated for 2 min at room temperature. The mixture was then centrifuged at 12,000 rpm for 15 min at 4 °C. The aqueous upper phase was transferred to a new tube, and an equal volume of 70% ethanol was added and mixed well. The phase liquid was transferred to the RNeasy Mini spin column and was centrifuged at 12,000 rpm for 15 s, and the flow-through was discarded. Then, 350 µL of buffer RW1 was added to the column and centrifuged, and the flow-through was discarded. A total of 500 µL of buffer RPE was added to the column and centrifuged, and the flow-through was discarded. The buffer RPE step was repeated. The column was placed in a fresh collection tube and was dry centrifuged for 2 min at maximum speed. Finally, the column was placed in a new microcentrifuge tube and eluted with the appropriate amount (~50 µL) of RNase-free water.
Full text: Click here
In this section, we detail the extraction of total RNA, involving microRNAs, from the specimens utilizing the TRIzol™ reagent. The extraction procedure adhered to the manufacturer's protocol provided by Thermo Fisher (USA).
Full text: Click here
Total RNA (including miRNAs) from fresh stool samples was extracted using the TRIzol method according to the manufacturer’s instructions, and minor modifications were applied (TRIzol, Invitrogen, Waltham, MA, USA). A similar amount of stool was homogenized with the TRIzol reagent, and the aqueous phase was collected in a new collection tube after centrifugation. This procedure was repeated 3 times. In the first round, 0.2 mL of chloroform was added; in the second wash, 0.5 mL of isopropanol was added; and in the third wash, 1 mL of 100% ethanol was added. The RNA pellet was then resuspended in RNase-free water. No silica-based membrane and no microspin technology were used for this extraction method (Figure 1 ).
Full text: Click here
One day prior to inactivation, BHK-21 cells in 6-well plates were infected with MADV-1 at an MOI of 1.0. The following day, the media was removed, and cells were washed with PBS. We added 1 mL of TRIzol (Sigma Aldrich, St. Louis, MO, USA) per well, or about 1 × 106 cells. Cells were collected in screw-cap tubes and incubated at room temperature for 15 min. Inactivations were performed in biological triplicates. Viable virus was detected using plaque assays and also assessed using the 13-day passage experiment.
Full text: Click here
Top products related to «Trizol»
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
Sourced in United States, Germany, China, Japan, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Belgium, Denmark, Netherlands, India, Ireland, Lithuania, Singapore, Sweden, Norway, Austria, Brazil, Argentina, Hungary, Sao Tome and Principe, New Zealand, Hong Kong, Cameroon, Philippines
TRIzol is a monophasic solution of phenol and guanidine isothiocyanate that is used for the isolation of total RNA from various biological samples. It is a reagent designed to facilitate the disruption of cells and the subsequent isolation of RNA.
Sourced in Japan, China, United States, France, Germany, Switzerland, Canada, Sweden, Italy, Puerto Rico, Singapore
The PrimeScript RT reagent kit is a reverse transcription kit designed for the synthesis of first-strand cDNA from RNA templates. The kit includes RNase-free reagents and enzymes necessary for the reverse transcription process.
Sourced in United States, Germany, United Kingdom, Japan, Lithuania, France, Italy, China, Spain, Canada, Switzerland, Poland, Australia, Belgium, Denmark, Sweden, Hungary, Austria, Ireland, Netherlands, Brazil, Macao, Israel, Singapore, Egypt, Morocco, Palestine, State of, Slovakia
The High-Capacity cDNA Reverse Transcription Kit is a laboratory tool used to convert RNA into complementary DNA (cDNA) molecules. It provides a reliable and efficient method for performing reverse transcription, a fundamental step in various molecular biology applications.
Sourced in Japan, China, United States, Germany, Puerto Rico
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other components that is used for the isolation of total RNA from cells and tissues. It facilitates the separation of RNA from DNA and proteins during the RNA extraction process.
Sourced in Germany, United States, United Kingdom, Netherlands, Spain, Japan, Canada, France, China, Australia, Italy, Switzerland, Sweden, Belgium, Denmark, India, Jamaica, Singapore, Poland, Lithuania, Brazil, New Zealand, Austria, Hong Kong, Portugal, Romania, Cameroon, Norway
The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
Sourced in United States, Germany, Canada, China, France, United Kingdom, Japan, Netherlands, Italy, Spain, Australia, Belgium, Denmark, Switzerland, Singapore, Sweden, Ireland, Lithuania, Austria, Poland, Morocco, Hong Kong, India
The Agilent 2100 Bioanalyzer is a lab instrument that provides automated analysis of DNA, RNA, and protein samples. It uses microfluidic technology to separate and detect these biomolecules with high sensitivity and resolution.
Sourced in Japan, China, United States, Switzerland, Germany, Australia, Canada, United Kingdom, France, Singapore, Puerto Rico, Sweden
SYBR Premix Ex Taq is a ready-to-use solution for real-time PCR amplification and detection. It contains all the necessary components, including DNA polymerase, dNTPs, and SYBR Green I dye, for efficient and accurate gene quantification.
Sourced in United States, Germany, Italy, Canada, United Kingdom, France, Netherlands, Switzerland, Sweden, Belgium, Japan, Australia, China, India, Spain, Denmark, Austria, Norway
The IScript cDNA Synthesis Kit is a reagent kit used for the reverse transcription of RNA into complementary DNA (cDNA). The kit contains all the necessary components to perform this reaction, including a reverse transcriptase enzyme, reaction buffer, and oligo(dT) primers.
Sourced in United States, Japan, China, Germany, United Kingdom, Switzerland, Canada, Singapore, Italy, France, Belgium, Denmark, Spain, Netherlands, Lithuania, Estonia, Sweden, Brazil, Australia, South Africa, Portugal, Morocco
The StepOnePlus Real-Time PCR System is a compact, flexible, and easy-to-use instrument designed for real-time PCR analysis. It can be used to detect and quantify nucleic acid sequences.
More about "Trizol"
Trizol, also known as TRIzol, is a powerful reagent widely used in molecular biology and genomics research for the extraction and purification of total RNA from a variety of biological samples.
This monophasic solution, composed of phenol, guanidine isothiocyanate, and other proprietary components, efficiently lyses cells and denatures cellular components to release intact RNA.
Trizol-based protocols are renowned for their high-yield and high-quality RNA isolation, enabling a wide range of downstream applications such as RT-PCR, Northern blotting, and RNA-seq.
The ease of use, versatility, and reproducibility of Trizol make it an indispensable tool for researchers seeking to accurately quantify and analyze gene expression profiles across diverse experimental systems.
In addition to Trizol, related reagents and kits, such as the PrimeScript RT reagent kit, High-Capacity cDNA Reverse Transcription Kit, RNeasy Mini Kit, and IScript cDNA synthesis kit, are commonly used in conjunction with Trizol for complementary RNA isolation, reverse transcription, and cDNA synthesis steps.
Furthermore, the Agilent 2100 Bioanalyzer and SYBR Premix Ex Taq are often utilized for quality assessment and real-time PCR analysis of the isolated RNA, respectively.
By leveraging the insights gained from the MeSH term description and the metadescription, researchers can enhance the reproducibility and accuracy of their studies, optimizing their workflows and ensuring the integrity of their gene expression data.
PubCompare.ai's AI-driven platform can assist in this process by helping researchers locate the best Trizol protocols from literature, pre-prints, and patents, empowering their research with unparalleled precision and efficiency.
This monophasic solution, composed of phenol, guanidine isothiocyanate, and other proprietary components, efficiently lyses cells and denatures cellular components to release intact RNA.
Trizol-based protocols are renowned for their high-yield and high-quality RNA isolation, enabling a wide range of downstream applications such as RT-PCR, Northern blotting, and RNA-seq.
The ease of use, versatility, and reproducibility of Trizol make it an indispensable tool for researchers seeking to accurately quantify and analyze gene expression profiles across diverse experimental systems.
In addition to Trizol, related reagents and kits, such as the PrimeScript RT reagent kit, High-Capacity cDNA Reverse Transcription Kit, RNeasy Mini Kit, and IScript cDNA synthesis kit, are commonly used in conjunction with Trizol for complementary RNA isolation, reverse transcription, and cDNA synthesis steps.
Furthermore, the Agilent 2100 Bioanalyzer and SYBR Premix Ex Taq are often utilized for quality assessment and real-time PCR analysis of the isolated RNA, respectively.
By leveraging the insights gained from the MeSH term description and the metadescription, researchers can enhance the reproducibility and accuracy of their studies, optimizing their workflows and ensuring the integrity of their gene expression data.
PubCompare.ai's AI-driven platform can assist in this process by helping researchers locate the best Trizol protocols from literature, pre-prints, and patents, empowering their research with unparalleled precision and efficiency.