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Tween 60

Tween 60 is a nonionic surfactant commonly used in biochemical and cell culture applications.
It is a polyoxyethylene sorbitan monostearate, with a hydrophile-lipophile balance (HLB) value of 14.9, making it suitable for emulsifying and solubilizing a wide range of compounds.
Tween 60 is known for its low toxicity and is often employed to enhance the solubility and stability of proteins, enzymes, and other biomolecules.
Its mild detergent properties make it useful in cell lysis, immunoassays, and other laboratory procedures that require the dispersion or extraction of cellular components.
Tween 60 is also used in the formulation of pharmaceuticals, cosmetics, and personal care products to improve the solubility and dispersion of active ingredients.

Most cited protocols related to «Tween 60»

1
Preparation and Characterization of Lipid-based Nanoparticles
Cited 8 times
Stearic acid and SPAN 80 were purchased from Merck (Merck KGaA, Darmstadt, Germany). Arachidic acid, Tween 60, Tween 80, poly(vinyl alcohol), L-lysine monohydrochloride, lithium carbonate, dansyl chloride, methylamine hydrochloride, triethylamine and sodium acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Miglyol 812 was purchased from Caelo (Caesar & Loretz GmbH, Hilden, Germany). Precirol ATO 5 and Compritol 888 ATO were kindly provided by Gattefossé (Saint Priest Cedex, France). L-Phenylalanine ethyl-ester hydrochloride was purchased from Fluka (Fluka Chemie GmbH, Buchs, Switzerland), acetic acid was obtained from VWR Chemicals (VWR International S.A.S., Fontenay-sous-Bois, France) and acetonitrile and methanol were obtained from Honeywell (Honeywell Riedel-de Häen AG, Seelze, Germany). Aqueous solutions were prepared with double-deionized water (Arium Pro, Sartorius AG, Göttingen, Germany).
Albuquerque J., Casal S., Páscoa R.N., Van Dorpe I., Fonseca A.J., Cabrita A.R., Neves A.R, & Reis S. (2020). Applying nanotechnology to increase the rumen protection of amino acids in dairy cows. Scientific Reports, 10, 6830.
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Publication 2020
Acetic Acid acetonitrile arachidic acid Cedax Compritol ATO 888 dansyl chloride L-phenylalanine ethylester Lithium Carbonate Lysine Methanol methylamine hydrochloride miglyol 812 Polyvinyl Alcohol precirol ATO 5 Sodium Acetate Span 80 stearic acid triethylamine Tween 60 Tween 80
2
Isolation and Characterization of Fusarium from Pinus in Colombia
Cited 5 times

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2015
Agar Conidia Diagnosis Ethanol Fusarium Glucose Infection Mycelium Nonalcoholic Steatohepatitis Pinus Plant Roots Plants Seedlings Sodium Hypochlorite Solanum tuberosum Spores Stem, Plant Sterility, Reproductive Tissues Trees Tween 60
3
Optimizing Niosomal Encapsulation of E. angustifolia
Cited 4 times
Design of Experiments 10.0.3 software (Stat-Ease Inc., Minneapolis, MN, USA) applying the Box-Behnken methodology was used to investigate the effect of self-governing variables (hydration time, hydration volume, and the cholesterol content; Table 1) on physicochemical features of E. angustifolia-loaded niosomes. Table 2 shows these factors, their degree, as well as their impacts on the nanoparticle size and entrapment efficiency (EE). The minimal size of the niosomes and the maximal entrapment efficiency were taken as the optimization criteria based on the multi-criteria optimization used [15 (link)]. The data optimization of the D-optimal study was performed based on the desirability index [16 (link)]. Furthermore, the discrepancies of the anticipated and the perceived results were computed, and the optimized formation was then discerned for more far-away studies [17 (link),18 (link),19 (link)].
The thin-layer hydration method described in our prior research with slight changes was used to prepare the E. angustifolia-loaded niosomes [20 (link)]. Briefly, Span 60, Tween 60, and cholesterol were suspended in an organic solvent (2:1 of chloroform:methanol (v/v), 10 mL), accompanied by evaporation of solvents using a rotary evaporator (150 rpm, 60 °C, 30 min). Subsequently, thin layers were hydrated, and 15 mg of the drug (concentration of 1.5 mg mL−1) with different hydration volumes and time (Table 1 and Table 2) was added at 60 °C and 120 rpm. Lastly, 7 min of sonication was performed to obtain the uniform size distribution of E. angustifolia-loaded niosomes. The specimens were refrigerated (4 °C) for further experimental research. The compositions of niosomal formation are listed in Table 2.
Moghtaderi M., Mirzaie A., Zabet N., Moammeri A., Mansoori-Kermani A., Akbarzadeh I., Eshrati Yeganeh F., Chitgarzadeh A., Bagheri Kashtali A, & Ren Q. (2021). Enhanced Antibacterial Activity of Echinacea angustifolia Extract against Multidrug-Resistant Klebsiella pneumoniae through Niosome Encapsulation. Nanomaterials, 11(6), 1573.
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Publication 2021
Chloroform Cholesterol Methanol Niosomes Pharmaceutical Preparations Solvents Span 60 Tween 60
4
Niosomal Delivery of Curcumin and Paclitaxel
Cited 4 times
We used the thin-film hydration method to prepare the curcumin and paclitaxel-loaded niosomes [47 (link)]. Tween-60 (DaeJung Chemicals & Metals, South Korea) and cholesterol (Sigma-Aldrich, USA) were dissolved in chloroform to obtain the different molar ratio molarities (as illustrated in Table 1). PTX (Stragen, Switzerland) and CUR (Sigma-Aldrich, USA) were dissolved in chloroform and added to the mixture of surfactant and lipids. Fluorescent label Dil (Sigma, USA) was added to the lipid phase at 0.1% mol for lipid staining to evaluate cellular uptake. Niosomal formulations were screened for particle size, controlled release, and high entrapment efficiency parameters. After attaining optimized synthetic conditions, the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane, Sigma-Aldrich, USA) and polyethylene glycol (Lipoid PE 18:0/18:0–PEG2000, DSPE-mPEG 2000, Lipoid GmbH, Germany) were added for improving stability and transfection efficiency of the niosomal formulations. Organic solvent was removed by rotary evaporator (Heidolph, Germany) at 50 °C until a thin-layered film formed. The dry lipid films were hydrated by adding phosphate-buffered saline (PBS, pH = 7.4) at 60 °C for 60 min to obtain the niosomal suspensions. After hydration, the prepared vesicles were sonicated for 30 min using a microtip probe sonicator (model UP200St, Hielscher Ultrasonics GmbH, Germany) to reduce the vesicles’ mean size. Thereafter, free drugs (unloaded) were separated from niosomal vesicles using a dialysis bag diffusion technique against PBS for 1 h at 4 °C (MW = 12 kDa, Sigma-Aldrich, USA) [48 (link)]. Drug-free niosomes were produced in a similar manner without adding curcumin and paclitaxel. The dose of both drugs was 0.5 mg mL−1 for all formulations.
Alemi A., Zavar Reza J., Haghiralsadat F., Zarei Jaliani H., Haghi Karamallah M., Hosseini S.A, & Haghi Karamallah S. (2018). Paclitaxel and curcumin coadministration in novel cationic PEGylated niosomal formulations exhibit enhanced synergistic antitumor efficacy. Journal of Nanobiotechnology, 16, 28.
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Publication 2018
1,2-dioleoyloxy-3-(trimethylammonium)propane 1,2-distearoylphosphatidylethanolamine Cations Cells Chloroform Cholesterol Curcumin Dialysis Diffusion Lipids Metals Molar monomethoxypolyethylene glycol Niosomes Paclitaxel Pharmaceutical Preparations Phosphates Polyethylene Glycols Propane Saline Solution Solvents Surfactants Transfection Tween 60 Ultrasonics
5
Hal-loaded Spanlastic Dispersions with Penetration Enhancers
Cited 4 times
Based on previous work of our research team (data under publication), two spanlastic formulae (F1 and F2) were chosen as a foundation for the preparation of PECSs formulae. They contained Span® 60 along with Tween® 80 as an edge activator (EA). F1: 400 mg Span® 60 and 100 mg of Tween® 80 (4:1 weight ratio) and F2: 300 mg of Span® 60 and 200 mg of Tween® 80 (3:2 weight ratio). Each formula had 25 mg of Hal and the total volume was 10 ml. These were prepared using ethanol injection method as described by Kakkar & Kaur (2011 (link)). Briefly, Hal and Span® 60 were dissolved in 2 ml ethanol and injected dropwise into a hot (60 °C) Tween® 80 solution. The resulting hydroalcoholic solution was continuously stirred on a magnetic stirrer (Model MSH-20 D; Witeg Labortechnik GmbH Germany) for 30 minutes at 800 rpm to completely evaporate any remaining ethanol and to form Hal-loaded aqueous spanlastic dispersions. Ultrasonic water-bath sonication (Crest ultrasonics Corp., Trenton, NJ, USA) was done for 5 min to obtain a suitable particle size. These formulae were prepared again, but with the incorporation of one of three penetration enhancers (PE): Labrasol®, Transcutol® P or Tetraglycol® (Ahad et al., 2009 (link); Mura et al., 2009 (link)). These were used at 1% w/v concentration (100 mg) (Sinha & Kaur, 2000 (link)) in the final dispersion and were dispersed in the edge activator (EA) solution during preparation. Thus, six PECS formulae were obtained.
Fahmy A.M., El-Setouhy D.A., Ibrahim A.B., Habib B.A., Tayel S.A, & Bayoumi N.A. (2017). Penetration enhancer-containing spanlastics (PECSs) for transdermal delivery of haloperidol: in vitro characterization, ex vivo permeation and in vivo biodistribution studies. Drug Delivery, 25(1), 12-22.
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Publication 2017
Bath Crista Ampullaris Ethanol Labrasol Span 60 Span 80 tetraglycol transcutol P Tween 60 Tween 80 Ultrasonics

Most recents protocols related to «Tween 60»

1
Proton Exchange Membrane Fuel Cell Gas Diffusion Layer
Not available on PMC !

Example 22

A method for preparing a gas diffusion layer for proton exchange membrane fuel cell, includes steps as follows:

    • (1) preparing the carbon fiber suspension;
    • mixing the carbon fiber dispersion with the fibrous binder dispersion, then adding the ceramic fiber of 1 mm length (zirconia fiber), and then shearing and dispersing at a high-speed rate of 1500 r/min to obtain the carbon fiber suspension;
    • wherein the carbon fiber dispersion consists of the carbon fiber, the dispersant and water;
    • wherein the fibrous binder dispersion consists of the fibrous binder, the dispersant and water;
    • wherein the viscosity of dispersion composed of the dispersant and water is 2000 Pa·s in the carbon fiber suspension;
    • wherein the dispersant is Tween 60; wherein the amount of the dispersant in the carbon fiber suspension is 1.5 wt % of the amount of water;
    • wherein the fibrous binder is the composite filament numbered F-4 in Table 1;
    • wherein the length of the carbon fiber is 10-20 mm, the aspect ratio of the carbon fiber is 100-3000, and the mass of carbon fibers with the aspect ratio in the interval [100, 500) accounts for 10 wt % of the total mass of carbon fibers, the mass of carbon fibers with the aspect ratio in the interval [500, 1000) accounts for 60 wt % of the total mass of carbon fibers, the mass of carbon fibers with the aspect ratio in the interval [1000, 2000) accounts for 25 wt % of the total mass of carbon fibers, and the mass of carbon fibers with the aspect ratio in the interval [2000, 3000] accounts for 5 wt % of the total mass of carbon fibers; wherein the amount of the carbon fiber in the carbon fiber suspension is 5 wt % of the amount of water;
    • wherein the amount of the ceramic fiber is 5 wt % of the amount of the carbon fiber;
    • (2) papermaking and drying the carbon fiber suspension to obtain the carbon fiber base paper;
    • wherein the drying temperature is 140° C. and the drying time is 5 min;
    • in the prepared carbon fiber base paper, wherein the content of the fibrous binder is 30 wt %;
    • (3) cross-linking and curing of the carbon fiber base paper (hot-pressing cross-linking);
    • wherein the temperature of hot-pressing cross-linking is 300° C., the time of hot-pressing cross-linking is 5 min, and the pressure applied to the carbon fiber base paper is 5 MPa;
    • (4) carbonizing and graphitizing the cross-linked carbon fiber base paper under the protection of argon to obtain a gas diffusion layer for proton exchange membrane fuel cell;
    • wherein the carbonization temperature is 1250° C. and the carbonization time is 15 min; wherein the graphitization temperature is 2000° C. and the graphitization time is 5 min.

The prepared gas diffusion layer for proton exchange membrane fuel cell has hydrophilic channels composed of the ceramic fiber, and the pore gradient (that is, the pore size increases or decreases along the thickness direction), and the layer with the smallest pore size is the intrinsic microporous layer; wherein the gas diffusion layer for proton exchange membrane fuel cell has a thickness of 100 μm, a porosity of 70%, a contact angle with water of 145°, a tensile strength of 30 Ma, a normal resistivity of 70 mΩ·cm, an in-plane resistivity of 7 mΩ·cm, and a permeability of 2060 (mL·mm)/(cm2·h·mmAq).

US11876232B2. Gas diffusion layer for proton exchange membrane fuel cell and preparation method thereof (2024-01-16). DONGHUA UNIVERSITY [CN]. Inventors: Biao Wang [CN], Tongqing Qu [CN], Xiyi Huang [CN].
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Patent 2024
A 145 Argon Carbon Fiber Cytoskeletal Filaments Diffusion Fibrosis Permeability Pressure Protons Tween 60 Viscosity zirconium oxide
2
Immunolabeling and Live-cell Imaging of Oocytes
Oocytes or oocytes were fixed in a solution of 4% paraformaldehyde and 2% triton X-100 in PBS for 30 min at room temperature. Blocking was performed in PBS with 10% goat or donkey serum, 10% BSA and 2% Tween for 60 min at room temperature. Antibodies used for immuno-labelling: pPlk1 (1 : 200, Santa Cruz Biotechnology), N-Wasp (1 : 100, Cell Signaling Technology), TACC3 (AF5720-SP; R&D System), rat anti-tyrosinated α-tubulin (1 : 1000, Serotec, YL1/2) and FITC-conjugated mouse anti-α-tubulin (1 : 200, Alexa Fluor 488, Invitrogen) and detected with secondary antibody of Goat anti-Rabbit IgG antibody (1 : 1000, Alexa Fluor 555, Invitrogen). Phalloidin Alexa Fluor 555 (1 : 20, Cell Signaling Technology) was used where appropriate to stain actin. DNA was labelled using 10 min incubation in Hoechst 33 342 (10 µg ml−1, Sigma-Aldrich). Serial Z sections of fixed oocytes/oocytes were acquired at room temperature in a glass-bottomed dish using laser-scanning confocal microscope imaging system (SP8, Leica). For live-cell imaging, the microinjected oocytes were incubated in M2 media at 37°C under mineral oil and imaged with Leica SP8 confocal microscope. In some live imaging experiments, chromosomes were labelled with 1 µM Sir-DNA (Spirochrome)
Yuen W.S., Zhang Q.H., Bourdais A., Adhikari D., Halet G, & Carroll J. (2023). Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes. Open Biology, 13(3), 220326.
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Publication 2023
Actins alexa fluor 488 Alexa Fluor 555 alpha-Tubulin Antibodies Antibodies, Anti-Idiotypic Cells Chromosomes Equus asinus Fever Fluorescein-5-isothiocyanate Goat Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulin G Microscopy, Confocal Microscopy, Confocal, Laser Scanning Mus Oil, Mineral Oocytes paraform Phalloidine Rabbits Serum Stains Triton X-100 Tween 60 WASL protein, human
3
Culturing and Utilizing Malassezia restricta

M. restricta strain KCTC 27527 was used in this study.23 (link) The fungal cells were grown in Leeming and Notman agar medium [0.5% (w/v) glucose, 0.01% (w/v) yeast extract, 1% (w/v) peptone, 0.8% (w/v) bile salt, 0.05% (w/v) glycerol monostearate, 0.1% (v/v) glycerol, 0.05% (v/v) Tween 60, 1.2% (w/v) agar, and 0.5% (v/v) whole fat cow milk] at 34 °C for 3 days, and used for mouse studies.23 (link)
Zeng S., Hartmann P., Park M., Duan Y., Lang S., Llorente C., Wang Y., Cabré N., Fouts D.E., Bacher P., Jung W.H., Stärkel P, & Schnabl B. (2023). Malassezia restricta promotes alcohol-induced liver injury. Hepatology Communications, 7(2), e0029.
Publication 2023
Agar Cells Glucose Glycerin Milk, Cow's Mus Peptones Salts, Bile Strains Tween 60 Yeast, Dried
4
Multiplex Autoantibody Profiling Assay
Serum or plasma samples were tested at 1:100 dilution in 0.05% PBS-Tween supplemented with 1% (w/v) BSA and transferred into 96-well plates in a randomized layout. The bead array was distributed into a 384-well plate (Greiner BioOne) by transfer of 5 μL bead array per well. A total of 45 μL of the 1:100 diluted sera was transferred into the 384-well plate containing the bead array. Samples were incubated for 60 minutes on a shaker at room temperature. Beads were washed with 3 × 60 μL PBS-Tween on a plate washer (EL406, Biotek), and 50 μL of 1:1,000 diluted R-phycoerythrin–conjugated (R-PE–conjugated) Fc-γ–specific goat anti–human IgG F(ab’)2 fragment (Jackson ImmunoResearch, 106-116-098) was added to the 384-well plate for detection of bound human IgG. After incubation with the secondary antibody for 30 minutes, the plate was washed with 3 × 60 μL PBS-Tween and resuspended in 50 μL PBS-Tween prior to analysis using a FlexMap3D instrument (Luminex Corp.). Binding events were displayed as MFI. All samples were run in duplicate in each experiment. Longitudinal samples that showed new-onset autoantibodies were reanalyzed in duplicate on new bead arrays to confirm results. Samples from patients with COVID-19 were heat inactivated prior to analysis, as previously described (41 (link)).
Feng A., Yang E.Y., Moore A.R., Dhingra S., Chang S.E., Yin X., Pi R., Mack E.K., Völkel S., Geßner R., Gündisch M., Neubauer A., Renz H., Tsiodras S., Fragkou P.C., Asuni A.A., Levitt J.E., Wilson J.G., Leong M., Lumb J.H., Mao R., Pinedo K., Roque J., Richards C.M., Stabile M., Swaminathan G., Salagianni M.L., Triantafyllia V., Bertrams W., Blish C.A., Carette J.E., Frankovich J., Meffre E., Nadeau K.C., Singh U., Wang T.T., Luning Prak E.T., Herold S., Andreakos E., Schmeck B., Skevaki C., Rogers A.J, & Utz P.J. (2023). Autoantibodies are highly prevalent in non–SARS-CoV-2 respiratory infections and critical illness. JCI Insight, 8(3), e163150.
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Publication 2023
anti-IgG Autoantibodies COVID 19 Goat Homo sapiens Immunoglobulin Fc Fragments Immunoglobulins Patients Phycoerythrin Plasma Serum Technique, Dilution Tween 60 Tweens
5
Development and Characterization of Clarithromycin Liposomes
Clarithromycin was obtained as a gift sample from the JOSWE Pharmaceutical Company (Amman, Jordan). Phosphoric acid was purchased from RomiL™ (Cambridge, UK), while HPLC-grade acetonitrile and HPLC-grade methanol were purchased from Tedia™ (Fairfield, OH, USA). Span™ 40, Span™ 60, Span™ 80, Tween™ 60, Tween™ 80, and cholesterol (Chol) were purchased from Sigma Aldrich™ (Gillingham, Dorset, UK). Diethyl ether and methanol were purchased from Tedia™ (Fairfield, OH, USA). Cellulose dialysis membranes with a molecular weight cut-off (MWCO) of 12–14 kDa, average flat width of 28.46 mm, and average diameter of 17.5 mm were purchased from HiMedia Laboratories™ (Mumbai, Maharashtra, India). Polyethylene glycol 400 (PEG 400) was purchased from GCC Diagnostics™ (Deeside, Flintshire, UK). Hydroxypropyl methyl cellulose (HPMC) 40,000, dicetyl phosphate (DCP), and phosphate-buffered saline (PBS) tablets were purchased from Sigma Aldrich™ (Gillingham, Dorset, UK). All other chemicals used in this study were of analytical grade.
Zaid Alkilani A., Musleh B., Hamed R., Swellmeen L, & Basheer H.A. (2023). Preparation and Characterization of Patch Loaded with Clarithromycin Nanovesicles for Transdermal Drug Delivery. Journal of Functional Biomaterials, 14(2), 57.
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Publication 2023
acetonitrile Cellulose Cholesterol Clarithromycin Diagnosis Dialysis dicetylphosphate Ethyl Ether High-Performance Liquid Chromatographies Hypromellose Methanol Pharmaceutical Preparations Phosphates Phosphoric Acids polyethylene glycol 400 Saline Solution Span 40 Span 60 Span 80 Tissue, Membrane Tween 60 Tween 80

Top products related to «Tween 60»

1
Tween 60 by Merck Group
Sourced in Germany, United States, France, United Kingdom, Switzerland
Tween 60 is a non-ionic surfactant used in various laboratory applications. It is a polyoxyethylene sorbitan monostearate compound.
2
Span 60 by Merck Group
Sourced in United States, Germany, United Kingdom, Brazil, China, India, Spain, Switzerland, France
Span 60 is a non-ionic surfactant compound used in various laboratory applications. It functions as a wetting agent and emulsifier to facilitate the mixing and dispersion of substances in liquid environments.
3
Tween 80 by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, France, Sao Tome and Principe, Spain, Poland, China, Belgium, Brazil, Switzerland, Canada, Australia, Macao, Ireland, Chile, Pakistan, Japan, Denmark, Malaysia, Indonesia, Israel, Saudi Arabia, Thailand, Bangladesh, Croatia, Mexico, Portugal, Austria, Puerto Rico, Czechia
Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
4
Dimethyl sulfoxide (dmso) by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh
DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Tween 20 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, France, China, Spain, Australia, Japan, India, Poland, Sao Tome and Principe, Switzerland, Macao, Belgium, Canada, Denmark, Israel, Mexico, Netherlands, Singapore, Austria, Ireland, Sweden, Argentina, Romania
Tween 20 is a non-ionic detergent commonly used in biochemical applications. It is a polyoxyethylene sorbitan monolaurate, a surfactant that can be used to solubilize and stabilize proteins and other biomolecules. Tween 20 is widely used in various laboratory techniques, such as Western blotting, ELISA, and immunoprecipitation, to prevent non-specific binding and improve the efficiency of these assays.
7
Methanol by Merck Group
Sourced in Germany, United States, Italy, India, United Kingdom, China, France, Poland, Spain, Switzerland, Australia, Canada, Sao Tome and Principe, Brazil, Ireland, Japan, Belgium, Portugal, Singapore, Macao, Malaysia, Czechia, Mexico, Indonesia, Chile, Denmark, Sweden, Bulgaria, Netherlands, Finland, Hungary, Austria, Israel, Norway, Egypt, Argentina, Greece, Kenya, Thailand, Pakistan
Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
8
Chloroform by Merck Group
Sourced in United States, Germany, United Kingdom, India, Italy, Spain, France, Canada, Switzerland, China, Australia, Brazil, Poland, Ireland, Sao Tome and Principe, Chile, Japan, Belgium, Portugal, Netherlands, Macao, Singapore, Sweden, Czechia, Cameroon, Austria, Pakistan, Indonesia, Israel, Malaysia, Norway, Mexico, Hungary, New Zealand, Argentina
Chloroform is a colorless, volatile liquid with a characteristic sweet odor. It is a commonly used solvent in a variety of laboratory applications, including extraction, purification, and sample preparation processes. Chloroform has a high density and is immiscible with water, making it a useful solvent for a range of organic compounds.
9
Tween 40 by Merck Group
Sourced in United States, Germany, Spain, Australia
Tween 40 is a non-ionic surfactant commonly used in laboratory settings. It is a polyoxyethylene sorbitan monopalmitate, which functions as a wetting agent, emulsifier, and detergent.
10
Cholesterol by Merck Group
Sourced in United States, Germany, United Kingdom, India, Japan, Sao Tome and Principe, China, France, Spain, Canada, Switzerland, Italy, Australia, Israel, Brazil, Belgium, Poland, Hungary, Macao
Cholesterol is a lab equipment product that measures the concentration of cholesterol in a given sample. It provides quantitative analysis of total cholesterol, HDL cholesterol, and LDL cholesterol levels.

More about "Tween 60"

Tween 60 is a non-ionic surfactant, also known as polyoxyethylene sorbitan monostearate, that is widely used in biochemical and cell culture applications.
With a hydrophile-lipophile balance (HLB) value of 14.9, Tween 60 is effective in emulsifying and solubilizing a variety of compounds, making it a versatile tool for researchers.
Owing to its low toxicity, Tween 60 is often employed to enhance the solubility and stability of proteins, enzymes, and other biomolecules.
Its mild detergent properties make it useful in cell lysis, immunoassays, and other laboratory procedures that require the dispersion or extraction of cellular components.
Tween 60 shares similarities with other non-ionic surfactants, such as Span 60, Tween 80, and Tween 20, each with its own unique properties and applications.
DMSO, a commonly used solvent, and FBS, a crucial component in cell culture media, are often used in conjunction with Tween 60 to improve solubility and support cell growth.
In addition to its use in biochemical and cell culture applications, Tween 60 is also found in the formulation of pharmaceuticals, cosmetics, and personal care products, where it helps to improve the solubility and dispersion of active ingredients.
When working with Tween 60, it's important to consider potential interactions with other solvents, such as methanol and chloroform, as well as Tween 40 and cholesterol, which may affect the surfactant's behavior and the overall experimental outcomes.
By understanding the properties and applications of Tween 60, researchers can optimize their experimental protocols, enhance reproducibility, and streamline their research efforts using PubCompare.ai, the leading AI platform for research optimization.