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U 73122

U 73122 is a pharmacological agent that inhibits phospholipase C, a key enzyme involved in signal transduction pathways.
It is commonly used in research to investigate the role of phospholipid signaling in various biological processes.
PubCompare.ai, an AI-driven platform, can help optimize your U 73122 research protocols and enhance the reproducibility of your experiments.
The tool allows you to easily locate relevant protocols from literature, preprints, and patents, and leverages AI-driven comparisons to identify the most effective protocols and products for your research needs.
Streamline your U 73122 experiments and unlock new insights with PubCompare.ai's cutting-edg tools.

Most cited protocols related to «U 73122»

1
Protrusion Formation Induced by Chemoattractant Flow
Cited 7 times
A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump, (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure required for the chemoattractant flow. U73122 was used to inhibit PLC, and U73343 was used as a control. To induce the formation of protrusion, a micropipette was filled with 25 nM EGF and was placed ∼5 μm from the edge of a quiescent cell, and a pressure of 16 hPa was exerted to induce flow. Time-lapse series were taken using 20× NA 1.4 infinity-corrected optics on a microscope and analyzed in ImageJ. Protrusion is measured along three lines emanating from the cell centroid: (1) the front protrusion is measured along the axis formed by the centroid and the tip of the pipette; (2) the side protrusion is measured along the perpendicular to the latter; and (3) the back protrusion is measured along the axis forming 180° with the front axis. All measurements are standardized over the corresponding distance between the centroid and the cell periphery along the corresponding axis before the introduction of the pipette.
Mouneimne G., Soon L., DesMarais V., Sidani M., Song X., Yip S.C., Ghosh M., Eddy R., Backer J.M, & Condeelis J. (2004). Phospholipase C and cofilin are required for carcinoma cell directionality in response to EGF stimulation. The Journal of Cell Biology, 166(5), 697-708.
Publication 2004
Cardiac Arrest Chemotactic Factors Epistropheus Eye Microscopy Pressure U 73122
2
Evaluating Endocytic Pathways of Nanoparticles
Cited 6 times
HeLa or MCF10A cells were seeded in a 48 well plate at a density of ~2×104 cells/well 24 h prior to the experiment. On the following day, cells were washed one time with phosphate buffered saline (PBS) and incubated with following endocytic inhibitors in serum free media for 1 h at 37 °C: cytochalasin D (10 μg/mL), methyl-β-cyclodextrin (5 mg/mL), nocodazole (10 μg/mL), 3 mg/mL NaN3/50 mM 2-deoxyglucose, wortmannin (100 ng/mL), pertussis toxin (100 ng/mL), U-73122 (4 μg/mL), polyinosinic acid (10 μg/mL), chlorpromazine (10 μg/mL) and dynasore (80 μM). The concentrations of the inhibitors were used as described in previous reports.[39 (link)] After 1 h, media were replaced with fresh media containing the inhibitors with NPs (100 nM) and further incubated for 1 h at 37 °C. Untreated cells and cells treated with only NPs (no inhibitor) were used as negative and positive controls, respectively. After incubation, cells were washed three times with PBS and lysis buffer was added to each well. All lysed cell samples were then further processed for ICP-MS analysis (vide infra) to determine the intracellular amount of gold. All inhibitors were purchased from Sigma except for dynasore, chlorpromazine, and pertusis toxin that were obtained from Fisher Scientific. Particle uptake (%) was calculated based on the following equation:
NP uptake(%)=(NP uptake in presence of inhibitors∕NP uptake in absence of inhibitors)×100
Saha K., Kim S.T., Yan B., Miranda O.R., Alfonso F.S., Shlosman D, & Rotello V.M. (2012). Surface functionality of nanoparticles determines cellular uptake mechanisms in mammalian cells. Small (Weinheim an der Bergstrasse, Germany), 9(2), 300-305.
Publication 2012
3-deoxyglucose Acids Buffers Cells Chlorpromazine Culture Media, Serum-Free Cyclodextrins Cytochalasin D Gold HeLa Cells inhibitors N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide Nocodazole Pertussis Toxin Phosphates Protoplasm Saline Solution Sodium Azide Toxins, Biological U 73122 Wortmannin
3
Calcium-Sensing Receptor Signaling in Airway Smooth Muscle
Cited 5 times
fASM cells (or adult ASM cells) were grown to 50% confluence in 8-well chambers (Lab-Tek, Rochester, NY)) and serum starved. Since the intent of this study was to explore the CaSR which is sensitive to [Ca2+]o, cells were finally Ca2+-deprived for 12h prior to experimentation. Cells were loaded with the Ca2+ indicator Fura-2/AM (5 μM; ThermoFisher Scientific, Scotts Valley CA) for 30 min in 0 mM [Ca2+]o and then washed. Depending on the protocol, cells were then treated for 1h with either medium (vehicle), 10 μM calcimimetic (R568; Tocris/Bio-Techne, Minneapolis, MN), 10 μM calcilytic (NPS2143; Tocris), 1 μM PLC inhibitor (U73122; Tocris), 1 μM inhibitor of IP3 receptor (Xestospongin C; Sigma-Aldrich), or 100 μM ryanodine (to inhibit ryanodine receptor; Sigma-Aldrich) in media (DMEM F12) containing 0, 0.5, 1, or 2 mM [Ca2+]o. These procedures were done in separate sets of cells that were exposed to the same [Ca2+]o throughout, i.e. 0, 0.5, 1, or 2 mM Ca2+ HBSS. In separate experiments, the effect of PLC siRNA was explored (Dharmacon SMARTpool ON-TARGETplus siRNA; L-010280–00-0020), with appropriate vehicle (Lipofectamine) and nonsense siRNA controls.
Fura-2 loaded fASM or adult ASM cells were imaged on an inverted microscope (Nikon Eclipse Ti-U) using appropriate filters (340 and 380 nm excitation; 410 nM emission) with perfusion used to alter [Ca2+]o or add agonists as necessary: histamine (10 μM) or spermine (100 μM) in the appropriate [Ca2+]o environment. Baseline, peak, and amplitude of [Ca2+]i responses were recorded and analyzed.
Roesler A.M., Wicher S.A., Ravix J., Britt RD J.r., Manlove L., Teske J., Cummings K., Thompson M.A., Farver C., MacFarlane P., Pabelick C.M, & Prakash Y. (2019). Calcium Sensing Receptor in Developing Human Airway Smooth Muscle. Journal of cellular physiology, 234(8), 14187-14197.
Publication 2019
Adult agonists Cardiac Arrest Cells Fura-2 fura-2-am Hemoglobin, Sickle Histamine Lipofectamine Microscopy Mutation, Nonsense NPS 2143 Perfusion RNA, Small Interfering Ryanodine Ryanodine Receptors Serum Spermine Triphosphate Receptor, Inositol U 73122 xestospongin C
4
Electrophysiological Recording Protocols for Neuronal Ion Channel Studies
Cited 5 times
Normal aCSF (35 °C) and normal pipette solution were used in electrophysiological recording (Ibrahim et al., 2003 (link)) (Supplemental Methods). When the cationic blocker La3+ was added to the bath, a HEPES-buffered CSF solution was utilized (Zhang et al., 2008 (link)). In some experiments, low Na+ (5 mM) HEPES-buffered CSF solution and Ca2+ free extracellular CSF solution were used, where N-methyl-D-glucamine (NMDG) replaced Na+, and Mg2+ replaced Ca2+, respectively. For experiments measuring the ramp current-voltages (I–Vs) , K+-gluconate in the normal internal solution was replaced with Cs+-gluconate (pH 7.35 with CsOH), and the extracellular solution contained Na+, K+, Ih (HCN), Ca2+ and GABAA channel blockers (in mM: NaCl, 126; 4-aminopyridine, 5; KCl, 2.5; MgCl2, 1.2; CsCl, 2; CaCl2, 1.4; CoCl2, 1; nifedipine, 0.01; HEPES, 20; NaOH, 8; glucose, 10; tetrodotoxin, 0.001; picrotoxin, 0.1). Ramp I-Vs were also recorded in a solution that both Na+ and K+ were replaced with same concentration of Cs+(pH 7.35 with CsOH).
All drugs were purchased from Calbiochem (La Jolla, CA) unless otherwise specified. Leptin was provided by Dr. Parlow (Harbor-UCLA Medical Center, Torrance, California, USA) through the National Hormone and Peptide Program. The Jak2 inhibitor (E)-N-benzyl-2-cyano-3-(3,4-dihydroxyphenyl) acrylamide a-Cyano-(3,4-dihydroxy)-N-benzylcinnamide Tyrphostin B42 (AG 490), the PLC inhibitor U73122, the less active analog U73343 and the PI3 kinase inhibitor wortmannin (Alomone Laboratories) were dissolved in dimethylsulfoxide (DMSO) as stock solutions. The selective PLCγ inhibitor 1-O-Octadecyl-2-O-methyl-rac-glycero-3-phosphorylcholline (ET-18-OCH3) was dissolved in H2O. 1, 2-Bis-(o-aminophenoxyethane)-N, N, N', N'-tetra-acetic acid (BAPTA) tetrasodium salt was dissolved in the internal solution at a 10 mM concentration. The ion channel blockers/activators used were as follows: 1-[β-[3-(4-methoxyphenyl) propoxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride (SKF96365); 2-aminoethyl diphenylborinate (2-APB); flufenamic acid (FFA) and 1-(cis-9-Octadecenoyl)-2-acetyl-sn-glycerol, 2-Acetyl-1-oleoyl-sn-glycerol (OAG), all were dissolved in DMSO. LaCl3 was dissolved in H2O.
Qiu J., Fang Y., Rønnekleiv O.K, & Kelly M.J. (2010). Leptin Excites Proopiomelanocortin Neurons via Activation of TRPC Channels. The Journal of Neuroscience, 30(4), 1560-1565.
Publication 2010
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 1-Phosphatidylinositol 3-Kinase 2-aminoethyl diphenylborinate Acetic Acid Acrylamide ACTR protein, human AG-490 alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide Bath Cations cesium chloride Dalfampridine Flufenamic Acid gluconate Glucose Glycerin HEPES Hormones imidazole Ion Channel JAK2 protein, human Leptin Magnesium Chloride N-benzylcinnamide Nifedipine Peptides Pharmaceutical Preparations Picrotoxin PLCG2 protein, human SK&F 96365 Sodium Chloride Sulfoxide, Dimethyl Tetragonopterus Tetrodotoxin U 73122 Wortmannin
5
Immunocytochemistry of Transfected Cells
Cited 5 times
Liposome-based transfection reagents interfered with PI(4,5)P2 stainings (not shown). Therefore, for transient transfections, COS-7 cells were treated with the nonliposomal reagent Superfect (Qiagen). On the next day, cells were fixed for 30 min at 37°C, followed by 3–5 h at 4°C with 4% paraformaldehyde in DME with 2 mM EGTA. Subsequently, cells were further processed for immunocytochemistry as described in a previous study (Wiederkehr et al. 1997). The first antibody binding incubation was carried out overnight at 4°C, using an antibody dilution solution consisting of PBS, with 0.2% saponin, 50 mM glycine, 0.1% BSA, and 1% FCS. Cultured hippocampal neurons from wild-type and transgenic mice were labeled 24 h after plating, according to the same protocol.
For quantitative analysis of GMC and PI(4,5)P2 clusters, all cells from randomly selected fields (400×) with substantial levels of transgene expression (30–50% of all transgene expressing cells in any given field) were included in the analysis. At least 20 cells from one dish of transiently transfected cells were analyzed for every experiment, and the values are averages from at least two independent experiments. Images from 20 × 20 μm2 bins were captured and all clusters within the bin were analyzed with NIH Image software (see Fig. 2).
PC12B cells were transfected stably using the Fugene 6 reagent from Boehringer. Each experiment shown in the study was carried out with at least three independent clones, with similar results. For process outgrowth assays, 100,000 PC12 cells were plated on collagen-coated (30 μg/ml) 35-mm dishes, and, where indicated, the medium was changed 1 d after plating from DME, 10% horse serum, 5% FCS (growth medium) to DME, 1% horse serum, and 100 ng/ml NGF. Where process formation (>1 cell diameter) in the absence of NGF was monitored, cells were preincubated with or without neomycin for 2 h, replated in the presence or absence of the drug, and analyzed 3 h after replating. No preincubation was carried out when LiCl or the phospholipase C (PLC) inhibitor U-73122 was used.
To analyze the distribution of the actin cytoskeleton in PC12B clones, we determined intensity profiles of RITC-phalloidin labeling across randomly selected cells. Cells were plated on a collagen-coated substratum in the absence of NGF, fixed and stained 3 h after plating, photographed under identical conditions, and were analyzed with Image software. For each analyzed cell, one rectangular bin of 10 pixels in height was placed across the center of the cell (see also schematic in Fig. 6 B), and an edge-to-edge labeling intensity profile was collected. For each type of PC12B clone, such profiles had reproducible characteristic features of actin cytoskeleton distribution, as revealed when the profiles were superimposed. To highlight shared features, each of six independent profiles (i.e., six cells) was assigned the same light gray value, and overlapping areas were integrated (see Fig. 6 B).
Laux T., Fukami K., Thelen M., Golub T., Frey D, & Caroni P. (2000). Gap43, Marcks, and Cap23 Modulate Pi(4,5p)2 at Plasmalemmal Rafts, and Regulate Cell Cortex Actin Dynamics through a Common Mechanism. The Journal of Cell Biology, 149(7), 1455-1472.
Publication 2000
Biological Assay Cells Clone Cells Collagen COS-7 Cells Culture Media Egtazic Acid Equus caballus FuGene Glycine Hyperostosis, Diffuse Idiopathic Skeletal Immunocytochemistry Immunoglobulins Light Liposomes Mice, Transgenic Microfilaments Neomycin Neurons paraform PC12 Cells Pharmaceutical Preparations Phospholipase C Place Cells rhodamine B isothiocyanate phalloidin Saponin Serum Staining Technique, Dilution Transfection Transgenes Transients U 73122

Most recents protocols related to «U 73122»

1
Isolation of Extracellular Vesicles from Sympathetic Neurons
Sympathetic neurons from TrkAFLAG/FLAG animals were dissected and plated at 14,000 cells per microfluidic device as previously described36 (link),37 (link). At 6 DIV, 150 μL of complete media was added to the CB chamber and 100 μL of anti-FLAG-AF488 antibody (1:200) in complete media was added to the DA chamber. Conditioned media was collected from the CB chamber 15 h after the addition of the anti-FLAG-AF488 antibody and EVs were isolated. For inhibitor treatments, 100 μL of complete media containing inhibitors (LY294002 50 μM, U-73122 1 μM, or 0.1% v/v DMSO) was added to the CB chamber and 150 μL of anti-FLAG-AF488 antibody (1:200) in complete media was added the DA chamber. Conditioned media was collected from the CB chamber 15 h after the addition of the anti-FLAG-AF488 and inhibitors and EVs were isolated.
Mason A.J., Keeler A.B., Kabir F., Winckler B, & Deppmann C. (2023). Sympathetic neurons secrete retrogradely transported TrkA on extracellular vesicles. Scientific Reports, 13, 3657.
Full text: Click here
Publication 2023
Animals Antibodies, Anti-Idiotypic Cells Culture Media, Conditioned inhibitors LY 294002 Microchip Analytical Devices Neurons Sulfoxide, Dimethyl U 73122
2
CCK8 Cytotoxicity Assay for Keratinocytes
CCK8 method was used to analyze the effects of different concentrations of U73122, MK-2206 (2HCI), and PTX on keratinocytes. Briefly, the cells were seeded in 96-well plates at a density of 1.2 × 104 cells per well. After 24 h incubation, cells were respectively treated with various concentrations of PTX (100 ng/ml, 150 ng/ml, 200 ng/ml, 250 ng/ml, 300 ng/ml, and 350 ng/ml) for 4 h or U73122 (4.5 μM, 9 μM, 13.5 μM, 18 μM, 22.5 μM, and 27 μM) for 30 min or MK-2206 (2HCI) (3 μM,6 μM, 9 μM,12 μM, 15 μM, 18 μM, 21 μM, and 24 μM) for 2 h. Following the treatment, 10 μl CCK8 was added to each well, and 2 h later, absorbance was tested at a wavelength of 405 nm using a fluorescence spectrophotometer (BD Biosciences, San Jose, CA).
Lan Y., Zeng W., Wang Y., Dong X., Shen X., Gu Y., Zhang W, & Lu H. (2023). Opsin 3 mediates UVA-induced keratinocyte supranuclear melanin cap formation. Communications Biology, 6, 238.
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Publication 2023
Cells Fluorescence Keratinocyte MK 2206 U 73122
3
Preparation and Treatment of HEK Cells
U73122 (S8011, Selleck Chemicals, Houston, TX, USA) is dissolved in dimethyl sulfoxide (DMSO, D8371, Beijing, China), with the final concentration of 9 mM, and stored at −20 °C. For subsequent experiments, combined with cell viability results (see Supplementary data: Supplementary Fig s9a) and previous research results49 (link),54 (link), we selected 9 μM U73122 treatment of HEK for 15 min.
MK-2206 (2HCl) (S1078, Houston, TX, USA) is dissolved in dimethyl sulfoxide (DMSO, D8371, Beijing, China), with the final concentration of 12 mM, and stored at −80 °C. For subsequent experiments, combined with cell viability results (see Supplementary data: Supplementary Fig s9a) and previous research results55 (link), we selected 12 μM MK-2206 treatment of HEK for 24 h.
Pertussis toxin (PTX, P7208, Sigma-Aldrich St., Louis, USA) is dissolved in ddH2O, with the final concentration of 200 μg/ml, and stored at 4 °C. For subsequent experiments, combined with cell viability results (see Supplementary data: Supplementary Fig s9a) and previous research results48 (link), we selected 200 ng PTX treatment of HEK for 4 h.
Fluo-3 AM (F1241, Invitrogen, Thermo Fisher Scientific, USA) is dissolved in dimethyl sulfoxide (DMSO, D8371, Beijing, China), with the final concentration of 3 mM and stored at the light-free conditions at −20 °C.
Lan Y., Zeng W., Wang Y., Dong X., Shen X., Gu Y., Zhang W, & Lu H. (2023). Opsin 3 mediates UVA-induced keratinocyte supranuclear melanin cap formation. Communications Biology, 6, 238.
Full text: Click here
Publication 2023
Cell Survival Fluo-3 Light MK 2206 Pertussis Toxin Sulfoxide, Dimethyl U 73122
4
GLP-1 Secretion Assay in NCI-H716 Cells
NCI-H716 cells (1 × 106 cells/well) were seeded onto 12-well pre-coated plates and incubated for 48 h in siRNA solution diluted in DMEM to induce differentiation. After starvation, the cells were pre-treated with U73122 and 2-APB for 30 min. The medium was then exchanged with 1 mL/well DMEM containing 80 μM ISS, and the cells were incubated for 1 h. Then, the cell culture medium was centrifuged at 2000× g for 10 min to remove debris, and 50 μL of the supernatant was used for GLP-1 analysis. For the quantitative measurement of GLP-1 in the cell culture supernatants, a human GLP1 (7-36) enzyme-linked immunosorbent assay (ELISA) kit (ab184857) was purchased from Abcam (Cambridge, UK). The GLP-1 ELISA was performed according to the manufacturer’s protocol.
Lee S.H., Ko H.M., Jee W., Kim H., Chung W.S, & Jang H.J. (2023). Isosinensetin Stimulates Glucagon-like Peptide-1 Secretion via Activation of hTAS2R50 and the Gβγ-Mediated Signaling Pathway. International Journal of Molecular Sciences, 24(4), 3682.
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Publication 2023
Cell Culture Techniques Cells Culture Media EHMT1 protein, human Enzyme-Linked Immunosorbent Assay Glucagon-Like Peptide 1 Homo sapiens RNA, Small Interfering U 73122
5
Measurement of Cytosolic Calcium Dynamics
Differentiated NCI-H716 cells were seeded onto a clear-bottom 96-well black plates (Corning, Tewksbury, MA, USA) pre-coated with Matrigel. Before the experiment, the medium was replaced with PBS and incubated for 30 min with fura-2 AM dye (final concentration 1 μM) as described previously. Then, the medium was replaced with U73122 (10 μM, Sigma–Aldrich) and 2-aminoethoxydiphenyl borate (2-APB; 10 μM, Sigma–Aldrich) in fresh PBS for 30 min. Cytosolic free calcium [Ca2+]i was observed using a Nikon Eclipse TS 100 fluorescence imaging system (Nikon Instrument Inc., Melville, NY, USA). Alternating excitation wavelengths of 340 and 380 nm were delivered from a rotating wheel filter, and images were analyzed using InCyt Im2 software (Intracellular Imaging; Cincinnati, OH, USA).
Lee S.H., Ko H.M., Jee W., Kim H., Chung W.S, & Jang H.J. (2023). Isosinensetin Stimulates Glucagon-like Peptide-1 Secretion via Activation of hTAS2R50 and the Gβγ-Mediated Signaling Pathway. International Journal of Molecular Sciences, 24(4), 3682.
Full text: Click here
Publication 2023
2-aminoethoxydiphenyl borate Calcium Cells Cytosol fura-2-am matrigel Protoplasm U 73122

Top products related to «U 73122»

1
U73122 by Merck Group
Sourced in United States, Germany, Italy, France, Sao Tome and Principe, Japan, United Kingdom, Sweden
U73122 is a laboratory reagent produced by Merck Group. It functions as a phospholipase C inhibitor. Further details on its intended use or applications are not provided in this unbiased and factual description.
2
U73122 by Bio-Techne
Sourced in United Kingdom, United States
U73122 is a chemical compound that functions as a phospholipase C inhibitor. It is commonly used in biochemical and cell biology research applications.
3
Bapta am by Merck Group
Sourced in United States, Germany, Japan, China, France, United Kingdom, Israel, Macao
BAPTA-AM is a calcium chelator that can be used to control intracellular calcium levels in biological systems. It functions by rapidly binding to and sequestering calcium ions within the cell.
4
U73122 by Cayman Chemical
Sourced in United States
U73122 is a chemical compound that functions as a phospholipase C inhibitor. It is commonly used in research applications to study cellular signaling pathways.
5
U73343 by Bio-Techne
Sourced in United Kingdom, United States
U73343 is a laboratory reagent produced by Bio-Techne. It functions as a phospholipase C inhibitor. No further details on its intended use or application are provided.
6
2 apb by Merck Group
Sourced in United States, Germany, United Kingdom, Canada
2-APB is a chemical compound used as a laboratory reagent. It functions as an inhibitor of inositol 1,4,5-trisphosphate (IP3) receptors, which are responsible for the release of calcium from intracellular stores. The core function of 2-APB is to modulate cellular calcium signaling processes in various experimental settings.
7
Thapsigargin by Merck Group
Sourced in United States, United Kingdom, Germany, Sao Tome and Principe, Italy, France, Japan, Canada, Spain, Macao, Belgium, Switzerland
Thapsigargin is a naturally occurring compound isolated from the plant Thapsia garganica. It functions as a selective inhibitor of the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) pump, which is responsible for the active uptake of calcium ions into the endoplasmic reticulum. Thapsigargin is a valuable tool for researchers studying calcium signaling and homeostasis in biological systems.
8
U73122 by Selleck Chemicals
Sourced in United States, China
U73122 is a laboratory chemical compound used in biochemical research. It functions as a phospholipase C inhibitor. The core purpose of U73122 is to provide researchers with a tool to study cellular signaling pathways involving phospholipase C.
9
Fura 2 am by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Italy, France, Spain, Australia, Japan, China, Canada, Belgium, Austria, Hungary, Macao
Fura-2 AM is a fluorescent calcium indicator used for measuring intracellular calcium levels. It is a cell-permeable derivative of the parent compound Fura-2. Fura-2 AM can be loaded into cells, where intracellular esterases cleave off the acetoxymethyl (AM) ester group, trapping the Fura-2 indicator inside the cell.
10
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

More about "U 73122"

U-73122 is a widely used pharmacological agent that inhibits the enzyme phospholipase C, a key player in signal transduction pathways.
This compound is commonly employed in research to investigate the role of phospholipid signaling in various biological processes.
PubCompare.ai, an innovative AI-driven platform, can help optimize your U-73122 research protocols and enhance the reproducibility of your experiments.
This powerful tool allows you to easily locate relevant protocols from literature, preprints, and patents, and leverages AI-driven comparisons to identify the most effective protocols and products for your research needs.
In addition to U-73122, researchers may also utilize related compounds like BAPTA-AM, U-73343, 2-APB, Thapsigargin, and Fura-2 AM to study calcium signaling, endoplasmic reticulum (ER) function, and other cellular processes.
These chemical tools can provide valuable insights when used in conjunction with U-73122, helping to unravel the complexities of signal transduction pathways.
Streamline your U-73122 experiments and unlock new discoveries with the cutting-edge tools provided by PubCompare.ai.
This AI-powered platform can help you optimize your research protocols, improve experimental reproducibility, and accelerate your scientific progress.
Explore the vast library of protocols and leverage the power of AI-driven analysis to enhance your U-73122 research today.