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Ursolic acid
Ursolic acid
Ursolic acid is a naturally occurring pentacyclic triterpenoid compound found in various plants, including herbs, fruits, and medicinal plants.
It has been the subject of extensive research due to its diverse pharmacological properties, including anti-inflammatory, antioxidant, antimicrobial, and anticancer activities.
Ursolic acid has shown promise in the prevention and treatment of various diseases, such as cancer, cardiovascular disorders, metabolic syndromes, and neurological conditions.
Researchers continue to explore the mechanims of action and therapeutic potential of ursolic acid, as well as optimze research protocols to enhance the reproducibility and accuracy of results.
PubCompare.ai offers a unique solution to streamline ursolic acid research by leveraging artificial intelligence to identify the most effectie and reliable research methods from the scientific literature, preprints, and patents.
It has been the subject of extensive research due to its diverse pharmacological properties, including anti-inflammatory, antioxidant, antimicrobial, and anticancer activities.
Ursolic acid has shown promise in the prevention and treatment of various diseases, such as cancer, cardiovascular disorders, metabolic syndromes, and neurological conditions.
Researchers continue to explore the mechanims of action and therapeutic potential of ursolic acid, as well as optimze research protocols to enhance the reproducibility and accuracy of results.
PubCompare.ai offers a unique solution to streamline ursolic acid research by leveraging artificial intelligence to identify the most effectie and reliable research methods from the scientific literature, preprints, and patents.
Most cited protocols related to «Ursolic acid»
Anesthesia
Animals
Bilirubin
Biological Assay
Blood Glucose
Cholesterol
Corn oil
Creatinine
Fast Foods
Glucose
Hindlimb
Hormones
IGF1 protein, human
Institutional Animal Care and Use Committees
Leptin
Males
Metformin
Mice, House
Mice, Inbred C57BL
Muscle Denervation
Normal Saline
Plasma
Sciatic Nerve
Tail
Triglycerides
Upper Extremity
ursolic acid
Veins
The DPPH assay (DPPH free radicals) was used to find out the anti-radical properties of the samples (Ahmad et al., 2015 (link)). DPPH solution was prepared by adding 3.2 mg of it into 100 ml methanol (82%). Into a glass vial, 2,800 μl of DPPH solution was added followed by 200 μl of test sample. The serial dilutions were prepared in concentration of 1,000, 500, 250, 125, and 62.5 μg/ml.
The mixture was stirred and allowed to stay at room temperature in dark for 1 h. UV-Visible Spectrophotometer (Shimadzu 1,800) was used for recording of absorbance. A mixture of 82% methanol (2,800 μl) and 200 μl methanol was used as blank. Whilemethanol 200 and 2,800 μl of DPPH solution was used as a control. The test was repeated three (3) times. The % inhibition was find out by using the given formula.
Where Ac is the control absorbance and As is the test sample absorbance.
The mixture was stirred and allowed to stay at room temperature in dark for 1 h. UV-Visible Spectrophotometer (Shimadzu 1,800) was used for recording of absorbance. A mixture of 82% methanol (2,800 μl) and 200 μl methanol was used as blank. Whilemethanol 200 and 2,800 μl of DPPH solution was used as a control. The test was repeated three (3) times. The % inhibition was find out by using the given formula.
Where Ac is the control absorbance and As is the test sample absorbance.
Biological Assay
Free Radicals
Methanol
Psychological Inhibition
Technique, Dilution
Tumor volumes, body weights and percentage of apoptotic cells were represented as mean±s.d. Statistical analyses were performed using the two-tailed Student's t test. P<0.05 (*) or P<0.01 (**) were considered significant.
Apoptosis
Body Weight
Cells
Crossbreeding
Diet
Exons
Gene Expression
Homo sapiens
Microarray Analysis
Mus
RNA, Messenger
Skeletal Muscles
Spinal Cord Injuries
trizol
ursolic acid
SEM was performed on biofilm formed on glass coverslips (0.2 mm thick and 6 mm in diameter) by dispensing 700 μL of the cell suspensions into the wells of 24-well flat bottomed polystyrene plates (Costar 3524; Corning; USA). The plates were incubated at 37°C with shaking. The coverslips were then washed three times with water and fixed using 2.5% glutaraldehyde in 0.1 M sodium phosphate buffer (pH 7.2) for 4 h at 4°C. Next the coverslips were washed three times with 0.1 M sodium phosphate buffer for 15 min each, followed by three 50% ethanol (v/v) washed for 10 min each. The samples were freeze-dried for 5 h and ultimately coated with gold and palladium in an evaporator. The observations were usually performed with a scanning electron microscope (FEI Quanta 200; USA).
Biofilms
Buffers
Cells
Ethanol
Freezing
Glutaral
Gold
Palladium
Polystyrenes
Scanning Electron Microscopy
sodium phosphate
Most recents protocols related to «Ursolic acid»
50 mg (1 eq.) of ursolic acid and 5 equivalents of the acid were added to 1.5 mL of dimethyl carbonate (∼160 eq.) in a one-necked 10 mL round-bottom glass flask containing a magnetic stirrer bar and molecular sieve. The reaction was run at 90 °C for 24 hours. On the high temperature (150 °C) heating method, the reaction was run in 25 mL Teflon auto-cleave stainless steel at 150 °C for 6 hours. The resulting solution was extracted with 50% water/ethyl acetate to remove the acid. Na2SO4 was added to the organic layer to remove water, then Na2SO4 was removed by filtration and the solvent was removed by evaporation in vacuo to obtain the mix of products. The ursolic acid ester products were isolated by column chromatography with the gradient 1% to 60% ratio of ethyl acetate/hexane as a mobile phase ratio. The products were characterized by using 1H-NMR, 13C-NMR and MS.
Human neutrophils were seeded at 1 × 106 cells per 1 mL. The neutrophils were then stimulated with different concentrations of ursolic acid in the presence of a M5 cytokine mixture consisting of 1 ng/mL each of IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α diluted in RPMI 1640 medium. After 24 h, the cell medium was collected and stored at −20 °C for further analysis, and cells were harvested for flow cytometry analysis.
HaCaT cells were seeded on 48-well plates (100,000 cells/well) a day prior to the experiment and stimulated with different concentrations of ursolic acid formulations in the presence of an M5 cytokin mix consisting of 1 ng/mL of each IL-17A, IL-22, oncostatin M, IL-1α, and TNF-α (BioLegend, BioCourse.pl, Katowice, Poland) diluted in DMEM medium. After 24 h, cell media were collected and stored at −20 °C for further analysis.
HaCaT cells were seeded on 96-well plates (5000 cells/well) a day prior to the experiment. Cells were incubated with various concentrations of ursolic acid formulations in the presence of 1 ng/mL M5 cytokine mix diluted in DMEM medium supplemented with 1% FBS. After 72 h, the medium was removed, and cells were washed once with PBS and incubated with 200 µL/well of 0.5 mg/mL Thiazolyl Blue Tetrazolium Bromide (Merck, Poznań, Poland) in DMEM for up to 20 min at 37 °C/5% CO2. The medium was removed again, and formazan crystals were dissolved in 120 µL of isopropanol acidified with 5 mM hydrochloric acid (Avantor Performance Materials, Gliwice, Poland). Quantities of 90 µL of samples from each well were transferred to a new, transparent 96-well plate. Absorbance of samples was measured using Spectra Max Gemini EM (Molecular Devices, San Jose, CA, USA) at 570 nm. Results were calculated as a percentage of the untreated control (cells in DMEM medium).
A volume of 120 mL of sodium carbonate Na2CO3 7.5% (w/v) solution was added to 60 mL of deacidified WGPO. The mixture was stirred for 10 min, and the aqueous phase was separated via liquid–liquid extraction. The aqueous phase was acidified with 2 N hydrochloride acid at pH = 3, frozen, and lyophilized. To the solid residue, 20 mL of ethyl acetate was added. The mixture was vortexed for 1 min and placed in an ultrasonic bath (Branson Fisher Scientific 150 E Sonic Dismembrator, Marshall Scientific, Hampton, VA, USA) for 10 min. The samples were then shaken on an orbital shaker (Sko-DXL, Argolab, Carpi, Italy) at 600 rpm for 10 min and centrifuged at 9000 rpm for 10 min. The supernatants were collected and stored at 4 °C protected from light. The obtained pellets were re-extracted with 10 mL of ethyl acetate using the same procedure. Finally, the extracts obtained were evaporated to dryness under a light stream of nitrogen, reconstituted in dimethylsulfoxide (DMSO) at a concentration of 30 mg/mL, diluted with acetonitrile at a concentration of 5 mg/mL, and stored at −20 °C until analysis [24 (link)].
Top products related to «Ursolic acid»
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Ursolic acid is a natural pentacyclic triterpenoid compound that can be isolated from various plant sources. It is a lab equipment product offered by Merck Group. Ursolic acid has a molecular formula of C30H48O3 and a molecular weight of 456.69 g/mol. The compound exhibits physicochemical properties that are of interest for research and development purposes.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Oleanolic acid is a naturally occurring pentacyclic triterpenoid compound. It is a chemical compound that can be extracted from various plant sources. Oleanolic acid is commonly used as a reference standard or analytical reagent in laboratory settings.
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Acetonitrile is a colorless, volatile, flammable liquid. It is a commonly used solvent in various analytical and chemical applications, including liquid chromatography, gas chromatography, and other laboratory procedures. Acetonitrile is known for its high polarity and ability to dissolve a wide range of organic compounds.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
More about "Ursolic acid"
Ursolic acid, a naturally occurring pentacyclic triterpenoid, has garnered significant attention in the scientific community due to its diverse pharmacological properties.
This compound, found in various plants such as herbs, fruits, and medicinal plants, has demonstrated potent anti-inflammatory, antioxidant, antimicrobial, and anticancer activities.
Researchers have extensively studied the mechanisms of action and therapeutic potential of ursolic acid, exploring its applications in the prevention and treatment of a wide range of diseases, including cancer, cardiovascular disorders, metabolic syndromes, and neurological conditions.
The compound's versatility has led to a growing body of research, with scientists continuously working to optimize research protocols and enhance the reproducibility and accuracy of their findings.
To streamline ursolic acid research, PubCompare.ai offers a unique solution that leverages artificial intelligence (AI) to identify the most effective and reliable research methods from the scientific literature, preprints, and patents.
By utilizing machine learning algorithms, PubCompare.ai helps researchers locate the best protocols, ensuring their work is built upon the most reproducible and accurate methods.
In addition to ursolic acid, related compounds such as oleanolic acid, quercetin, and FBS (fetal bovine serum) often play a crucial role in ursolic acid research.
Common solvents and reagents, including acetonitrile, DMSO (dimethyl sulfoxide), methanol, formic acid, and TRIzol reagent, are also frequently employed in ursolic acid-related experiments.
By harnessing the power of AI-driven protocol optimization, researchers can enhance the quality and efficiency of their ursolic acid studies, ultimately advancing our understanding of this remarkable compound and its therapeutic potential.
This compound, found in various plants such as herbs, fruits, and medicinal plants, has demonstrated potent anti-inflammatory, antioxidant, antimicrobial, and anticancer activities.
Researchers have extensively studied the mechanisms of action and therapeutic potential of ursolic acid, exploring its applications in the prevention and treatment of a wide range of diseases, including cancer, cardiovascular disorders, metabolic syndromes, and neurological conditions.
The compound's versatility has led to a growing body of research, with scientists continuously working to optimize research protocols and enhance the reproducibility and accuracy of their findings.
To streamline ursolic acid research, PubCompare.ai offers a unique solution that leverages artificial intelligence (AI) to identify the most effective and reliable research methods from the scientific literature, preprints, and patents.
By utilizing machine learning algorithms, PubCompare.ai helps researchers locate the best protocols, ensuring their work is built upon the most reproducible and accurate methods.
In addition to ursolic acid, related compounds such as oleanolic acid, quercetin, and FBS (fetal bovine serum) often play a crucial role in ursolic acid research.
Common solvents and reagents, including acetonitrile, DMSO (dimethyl sulfoxide), methanol, formic acid, and TRIzol reagent, are also frequently employed in ursolic acid-related experiments.
By harnessing the power of AI-driven protocol optimization, researchers can enhance the quality and efficiency of their ursolic acid studies, ultimately advancing our understanding of this remarkable compound and its therapeutic potential.