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Zinc Acetate

Zinc Acetate is an inorganic compound with the chemical formula Zn(CH3COO)2.
It is a white, crystalline solid that is soluble in water and commonly used in dietary supplements, pharmaceuticals, and industrial applications.
Reserach on Zinc Acetate has applications in areas such as wound healing, antioxidant activity, and zinc nutrional deficiency.
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Most cited protocols related to «Zinc Acetate»

Crystals were obtained at 16 °C for 7 days using the hanging drop vapor diffusion method by mixing equal volume of protein solution (HR1-L6-HR2, 10 mg/mL) and reservoir solution (10% PEG8000, 200 mM zinc acetate, 0.1 M MES, pH 6.0). Then crystals were flash-frozen and transferred to liquid nitrogen for data collection. On the in-house (Institute of Biophysics, Chinese Academy of Sciences) X-ray source (MicroMax 007 generator (Rigaku, Japan)) combined with Varimax HR optics (Rigaku, Japan), HR1-L6-HR2 crystals at 100 K were diffracted to 2.9-Å resolution at a wavelength of 1.5418 Å. A native set of X-ray diffraction data was collected with the R-AXIS IV++ detector (Rigaku, Japan) with an exposure time of 3 min per image and was indexed and processed using iMosflm.36 (link) The space group of the collected dataset is P21. Molecular replacement was performed with PHENIX.phaser37 (link) to solve the phasing problem, using the SARS-CoV S protein core structure (PDB code 1WYY) as a search model. The final model was manually adjusted in COOT and refined with Refmac.38 (link) Data collection statistics and refinement statistics are given in Table 1. Coordinates were deposited in the RCSB Protein Data Bank (PDB code: 6LXT). The interaction model of EK1C4 peptide and HR1 domains of SARS-nCoV-2 was predicted by SWISS-MODEL sever39 (link) using 6XLT as reference for EK1 moiety, and by Autodock 4 software40 (link) for cholesterol moiety (Supplementary information, Fig. S8).

Data collection and refinement statistics.

SARS-CoV-2 HR1-L6-HR2 PDB entry 6LXT
Data collection
 Space groupP 1 21 1
 Cell dimensions
   a, b, c (Å)51.2, 57.6, 115.7
   α, β, γ (°)90, 91.6, 90
 Wavelength (Å)1.5418
 Resolution (Å)47.32–2.90 (3.00–2.90)a
 Rmerge0.16 (1.13)
 Mean I/σ(I)6.3 (1.6)
 Completeness (%)95.2 (99.5)
 Redundancy7.1 (7.1)
Refinement
 Resolution (Å)47.32–2.90
 No. of reflections14313
 Reflections in test set737
 Rwork/Rfree0.259/0.290
 No. of atoms
   Protein5205
   Water & ligands32
 r.m.s. deviations
   Bond lengths (Å)0.013
   Bond angles (°)1.94
 Ramachandran outliers (%)0.15
 Average B-factor (Å2)87.99

aHighest resolution shell is shown in parenthesis.

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Publication 2020
Cells Chinese Cholesterol Complement Factor B Diffusion Epistropheus Eye Freezing Nitrogen Peptides polyethylene glycol 8000 Proteins Radiography Reflex SARS-CoV-2 spike glycoprotein, SARS-CoV X-Ray Diffraction Zinc Acetate
Sequences of the two PBM ‘all-10-mer’ designs are given at http://hugheslab.ccbr.utoronto.ca/supplementary-data/C2H2_modularity/. Details of the design and use of PBMs has been described elsewhere (41 (link),47 (link),49 (link),50 (link)). Plasmids are listed in Supplementary Table S1. ZFAs were cloned as SacI–BamHI fragments into pTH5325, a modified T7-driven GST expression vector (see Supplementary Document of the Supplementary Data). Briefly, we used 150 ng of plasmid DNA in a 25 μl in vitro transcription/translation reaction using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) supplemented with RNase inhibitor and 50 μM zinc acetate. After a 2-h incubation at 37°C, 12.5 μl of the mix was added to 137.5 μl of protein-binding solution for a final mix of PBS/2% skim milk/0.2 mg per ml BSA/50 μM zinc acetate/0.1% Tween-20. This mixture was added to an array previously blocked with PBS/2% skim milk and washed once with PBS/0.1% Tween-20 and once with PBS/0.01% Triton-X 100. After a 1-h incubation at room temperature, the array was washed once with PBS/0.5% Tween-20/50 μM zinc acetate and once with PBS/0.01% Triton-X 100/50 μM zinc acetate. Cy5-labeled anti-GST antibody was added, diluted in PBS/2% skim milk/50 μM zinc acetate. After a 1-h incubation at room temperature, the array was washed three times with PBS/0.05% Tween-20/50 μM zinc acetate and once with PBS/50 μM zinc acetate. The array was then imaged using an Agilent microarray scanner at 2 μM resolution.
Publication 2011
Antibodies, Anti-Idiotypic Cloning Vectors MBD protocol Microarray Analysis Milk, Cow's Plasmids Protein Biosynthesis Ribonucleases Transcription, Genetic Triton X-100 Tween 20 Zinc Acetate

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Publication 2014
adenosine 5'-O-(3-thiotriphosphate) Buffers Creatine Kinase Deoxyribonuclease I Dithiothreitol DNA Helicase A DNA Helicases Edetic Acid Egtazic Acid Glycerin HEPES HSP40 Heat-Shock Proteins Krypton magnesium acetate MCM2 protein, human Phosphocreatine Potassium Glutamate Proteins SDS-PAGE Sodium Chloride Stains Staphylococcal Protein A Zinc Acetate
Pristine zinc oxide nanocrystals (ZnO NCs) were synthetized by a wet chemical method using zinc acetate dihydrate (Zn(CH3COO)2·H2O) and sodium hydroxide (NaOH) as precursors and methanol as a solvent. In detail, 0.818 g (3.73 mmol) of Zn(CH3COO)2·H2O was dissolved in 42 mL of methanol in a 100 mL round bottom flask and heated to 60 °C under vigorous stirring. When the temperature reached 60 °C, 318 μL of bi-distilled water (from a Direct Q3 system, Millipore) and a solution of 0.289 g (7.22 mmol) of NaOH in 23 mL of methanol were dropwise added to the zinc acetate solution. The resulting synthesis mixture was maintained, under continuous stirring, at 60 °C for 2.15 h and then washed two times with fresh ethanol using a repeated centrifugation–redispersion process.
Publication 2017
Anabolism Centrifugation Ethanol Methanol ML 23 Sodium Hydroxide Solvents Suby's G solution Zinc Acetate Zinc Acetate Dihydrate Zinc Oxide
Details of the design and use of PBMs has been described elsewhere19 (link), 28 (link), 49 (link), 50 (link). Here, we used two different universal PBM array designs, designated ‘ME’ and ‘HK’, after the initials of their designers. Information about individual plasmids is available in Supplementary Table 8. We identified the DNA Binding Domain (DBD) of each TF by searching for Pfam domains51 (link) using the HMMER tool52 (link). DBD sequences along with 50 amino acid residues on either side of the DBD in the native protein were cloned as SacI–BamHI fragments into pTH5325, a modified T7-driven GST expression vector. Briefly, we used 150 ng of plasmid DNA in a 15 μl in vitro transcription/ translation reaction using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) supplemented with RNase inhibitor and 50 μM zinc acetate. After a 2-h incubation at 37°C, 12.5 ml of the mix was added to 137.5 ml of protein-binding solution for a final mix of PBS/2% skim milk/0.2 mg per ml BSA/50 μM zinc acetate/0.1% Tween-20. This mixture was added to an array previously blocked with PBS/2% skim milk and washed once with PBS/0.1% Tween-20 and once with PBS/0.01% Triton-X 100. After a 1-h incubation at room temperature, the array was washed once with PBS/0.5% Tween-20/50 mM zinc acetate and once with PBS/0.01% Triton-X 100/50 mM zinc acetate. Cy5-labeled anti-GST antibody was added, diluted in PBS/2% skim milk/50 mM zinc acetate. After a 1-h incubation at room temperature, the array was washed three times with PBS/0.05% Tween-20/50 mM zinc acetate and once with PBS/50 mMzinc acetate. The array was then imaged using an Agilent microarray scanner at 2 mM resolution. Images were scanned at two power settings: 100% photomultiplier tube (PMT) voltage (high), and 10% PMT (low). The two resulting grid images were then manually examined, and the scan with the fewest number of saturated spots was used. Image spot intensities were quantified using ImaGene software (BioDiscovery). PBM data are available at NCBI GEO under accession GSE42864.
Publication 2013
Acetate Amino Acid Sequence Antibodies, Anti-Idiotypic Cloning Vectors Exanthema Microarray Analysis Milk, Cow's ML 137 Plasmids Protein Biosynthesis Proteins Radionuclide Imaging Ribonucleases Transcription, Genetic Triton X-100 Tween 20 Zinc Acetate

Most recents protocols related to «Zinc Acetate»

Example 2

To quantify the encapsulation efficiency, the supernatant after crystals centrifugation was collected and remaining NGAL concentration was determined using sandwich enzyme-linked immunosorbent assay (ELISA). The encapsulation efficiency was found to be dependent on the concentration of ZIF-8 precursors. Specifically, when the concentrations of zinc acetate and 2-methylimidazole in the mixture increased to 40 mM and 160 mM, respectively, ˜95% NGAL was encapsulated within ZIF-8 crystals (FIG. 7). The encapsulation efficiency was calculated by subtracting the remaining NGAL amount in the supernatant after encapsulation and centrifugation (concentration measured by ELISA) from the total NGAL amount. Results are the mean and standard deviation from three independent experiments. As a control experiment, mixing of NGAL-spiked artificial urine with pure ZIF-8 crystals resulted in extremely low (˜10%, owing to the physical adsorption) encapsulation efficiency. This physical mixing of pre-formed ZIF-8 crystals with the protein biomarkers is in stark contrast with the protein-embedding approach (i.e. formation of ZIF-8 crystals in the presence of protein biomarkers), which exhibited high encapsulation efficiency (95%).

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Patent 2024
2-methylimidazole Adsorption Biological Markers Centrifugation crystal-8 Enzyme-Linked Immunosorbent Assay LCN2 protein, human Physical Examination Proteins Urine Zinc Acetate
After 5 days pre-conditioning, NO3- and sulfate reduction rates were determined for sediment from each core. Each core was placed in an anaerobic chamber under an N2 atmosphere and sediment was sampled from 4 to 10 cm above the core base and homogenized. Sediment was then transferred to Exetainers (Labco, Manchester) to create slurries whereupon labeling with 15N-NO3-, 35S-sulfate and subsequent sampling was carried out identically to the fresh sediment incubations. 35S-sulfate labeled samples from T0-T2 in the Nitrate Replete and Nitrate Deplete conditions, and T0 in the variably conditioned sediments were not weighed before they were decanted into zinc acetate, therefore the average sediment mass from other samples in their respective treatments was used for rate calculations.
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Publication 2023
Atmosphere Nitrates Sulfates, Inorganic Zinc Acetate
PVC core liners (I.D. 3.5 cm) were used to collect three vertical cores from the upper sand flat of Janssand during low tide on two occasions (October 17, 2018 and May 22, 2019) and transported to the lab (~2 h). In October surface water was approximately 14 °C, and in May 11 °C.
Cores were transferred to an anaerobic chamber and the upper pale (oxidized) layer (0–3 cm) was separated from a dark (reduced) layer (7–10 cm) (Supplementary Fig. 1). The upper layer was well mixed before 2 cm3 aliquots of sediment was transferred into 12 mL glass vials with septa (LabCo, Manchester), hereafter referred to as “Exetainers”, that were filled with filtered anoxic seawater collected October 10, 2018 (NO3- + NO2-- < 2 µM) creating sediment slurries. Exetainers were capped headspace free and removed from the anaerobic chamber whereupon they were assigned to one of three treatment groups (Supplementary Fig. 1). 38 Exetainers per core received 60 µM 15N-labeled NO3- (corresponding to ~300 nmol/cm3 sediment), 24 received 60 µM 15N-labeled NO3- and 250 kBq of 35S-labeled sulfate, 24 received only 250 kBq of 35S-labeled sulfate. Filled Exetainers were placed in roller tanks on a roller table. The roller table speed was set in order to gently invert the Exetainers every 44 seconds along their longitudinal axis to ensure that the slurries remained homogenous. Visual observations confirmed that this constantly mixed the sediment with the seawater in the vials.
Slurries were weighed and killed in duplicates at 12 selected time points with the aim of including timepoints before and after NO3- depletion. Slurries without added 35S (i.e. those with only 15N) were killed by injecting 100 µL 30% w/v zinc chloride and 200 µL saturated mercury chloride so that they were suitable for later 15N gas analysis. Slurries with added 35S were killed by first removing 1.8 mL sample water that was directly pipetted into 200 µL 20% w/v zinc acetate (total radioactivity samples) and stored at 4 °C, and the remaining sediment and water was decanted directly into 50 mL falcon tubes pre-filled with 7 mL 30% zinc acetate (TRIS samples) and frozen at −20 °C.
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Publication 2023
Anoxia Dental Cavity Liner Epistropheus Freezing Homozygote Mercuric Chloride Neoplasm Metastasis Radioactivity Sulfates, Inorganic Tromethamine Zinc Acetate zinc chloride
Lake Vechten (52°04’N, 5°05’E) is located in the center of The Netherlands, a few kilometers southeast of Utrecht, which is a large city with >350 000 people. The lake is located next to a golf court, squeezed between two highways and two major railways. The lake was formed by sand excavation to build the highway A12 in 1941 (Steenbergen and Verdouw 1982 ). There are no waste dumpsites and no ditches or streams connecting the lake with the city or the agricultural area east of the city. The lake is largely fed by groundwater and rainwater, including surface runoff from the golf court and the adjacent highway. The lake consists of two basins with a total surface area of 4.7 ha, and has a maximum depth of 11.9 m.
Vertical profiles of temperature, dissolved oxygen (DO), chlorophyll a, photosynthetically active radiation (PAR), specific conductivity, and pH of the lake water were measured in situ using a multiprobe Hydrolab DataSonde 4a (Hydrolab Corporation, Austin, TX, USA). From every meter depth in the Western Basin, water samples were collected monthly or biweekly from March 2013 to September 2014. Water was pumped via a hose connected to the Hydrolab Datasonde to make sure the water samples matched the conditions measured by the Hydrolab Datasonde at each particular depth. Water samples were filtered through 0.20 µm nylon membrane filters (Millipore, GNWP) to collect microorganisms. The filters were immediately frozen and stored at −20°C until further analysis. Sediment samples (0–10 cm) were collected monthly with a box-corer from the same location, transported to the laboratory in a dry shipper and stored at −20°C until further analysis. Dry weight of the sediment was determined after drying for 2 days at 60°C.
Subsequent to filtration, ammonium (NH4+), nitrite (NO2), nitrate (NO3), dissolved inorganic nitrogen (DIN), sulfate (SO42−), phosphate (PO43−), and chloride (Cl) were measured with an auto-analyzer (SAN++, Skalar, The Netherlands), while dissolved organic carbon (DOC) was measured with a total organic carbon analyzer (TOC-VCPH, Shimadzu, Japan). For sulfide (S2−) measurements, lake water was filtered through 0.20 µm polyethersulfone membrane filter and fixed with zinc acetate (10% w/v) immediately in the field. Afterward, sulfide was measured in the laboratory according to the methylene blue spectroscopic method (Trüper and Schlegel 1964 (link)). The data were visualized with Ocean Data View version 4.7.8 (Schlitzer 2002 ).
Publication 2023
Ammonium austin Carbon Chlorides Chlorophyll A Dissolved Organic Carbon Electric Conductivity Filtration Freezing Methylene Blue Nitrates Nitrites Nitrogen Nylons Oxygen Phosphates polyether sulfone Radiation Spectrum Analysis Sulfates, Inorganic Sulfides Tissue, Membrane Zinc Acetate
We analyzed patients who had been followed up until 12 weeks after, designating as a population those who had visited our outpatient department with post-COVID-19 conditions as a main complaint. The study period started on August 1, 2021 and ended on June 1, 2022. The analysis only included the existing data; there were no exclusion criteria. General practitioners were in charge of the examination. We confirmed the presence or absence of symptoms in patients using a self-reported check sheet (original sheet) at the time of medical interview (Supplementary Material 1, www.jocmr.org). According to World Health Organization, a “post-COVID-19 condition occurs in individuals with a history of probable or confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, usually 3 months from the onset of COVID-19 with symptoms that last for at least 2 months and cannot be explained by an alternative diagnosis.” We analyzed all post-morbid symptoms, however, regardless of symptom duration. Regarding hair loss, we used a loupe to check the condition of the scalp. For patients with hypozincemia, we prescribed 50 mg of zinc acetate hydrate tablets to be taken twice a day and continued until the patient healed. (In Japan, doctors cannot prescribe zinc acetate hydrate because it is not covered by insurance unless it is hypozincemia.)
Publication 2023
Alopecia COVID 19 Diagnosis General Practitioners Infection Outpatients Patients Physicians Post-Acute COVID-19 Syndrome SARS-CoV-2 Scalp Zinc Acetate

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Zinc acetate is a chemical compound with the formula Zn(CH3COO)2. It is a white, crystalline solid that is soluble in water and other polar solvents. Zinc acetate is commonly used as a laboratory reagent and in various industrial applications.
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Zinc acetate dehydrate is a chemical compound with the formula Zn(CH3COO)2·2H2O. It is a white crystalline solid that is soluble in water and organic solvents. Zinc acetate dehydrate is commonly used as a source of zinc in various applications, including pharmaceutical and chemical industries.
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Sodium hydroxide is a chemical compound with the formula NaOH. It is a white, odorless, crystalline solid that is highly soluble in water and is a strong base. It is commonly used in various laboratory applications as a reagent.
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Oleic acid is a long-chain monounsaturated fatty acid commonly used in various laboratory applications. It is a colorless to light-yellow liquid with a characteristic odor. Oleic acid is widely utilized as a component in various laboratory reagents and formulations, often serving as a surfactant or emulsifier.
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1-octadecene is a linear alkene with the molecular formula C18H36. It is a colorless, oily liquid that is commonly used as a chemical intermediate in various industrial and laboratory applications.
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Ethanol is a clear, colorless liquid chemical compound commonly used in laboratory settings. It is a key component in various scientific applications, serving as a solvent, disinfectant, and fuel source. Ethanol has a molecular formula of C2H6O and a range of industrial and research uses.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.
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Oleylamine is a chemical compound used as a surfactant, emulsifier, and lubricant in various industrial applications. It is a long-chain aliphatic amine with a hydrocarbon backbone and an amino group at one end. Oleylamine is commonly used in the formulation of lubricants, coatings, and personal care products.
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Zinc acetate is a chemical compound with the formula Zn(CH3COO)2. It is a white crystalline solid that is soluble in water and various organic solvents. Zinc acetate is commonly used as a source of zinc ions in various applications, such as in the manufacture of cosmetics, pharmaceuticals, and other industrial products.

More about "Zinc Acetate"

Zinc acetate, also known as zinc diacetate, is a versatile inorganic compound with the chemical formula Zn(CH3COO)2.
It is a white, crystalline solid that is soluble in water and commonly used in dietary supplements, pharmaceuticals, and various industrial applications.
Research on zinc acetate has unveiled its potential benefits in areas such as wound healing, antioxidant activity, and addressing zinc nutritional deficiencies.
Zinc acetate dehydrate is a related compound that contains two water molecules.
Sodium hydroxide, also called caustic soda, is often used in the production of zinc acetate, while oleic acid, 1-octadecene, and ethanol are common solvents or reagents.
Hydrochloric acid and methanol may also be involved in the synthesis or purification processes.
To optimize your zinc acetate research, PubCompare.ai can provide access to the best protocols from literature, preprints, and patents.
Their AI-driven comparisons can help identify the most effective methods and products, enhancing reproducibility, accuracy, and streamlining your research process for improved results.
By leveraging these insights, you can advance your understanding and applications of this versatile compound.