Mice were injected intraperitoneally with 1 × 106 zymosan or 1 × 105 live C. albicans and were killed after 4 h. The inflammatory infiltrate was collected by lavage with ice-cold 5 mM EDTA in PBS. Inflammatory cell populations were counted and then were analyzed by flow cytometry to determine the leukocyte composition as described19 (link),29 (link). Cytokines in peritoneal lavage fluid were determined as described above.
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Zymosan
Zymosan
Zymosan is a complex carbohydrate derived from the cell wall of the yeast Saccharomyces cerevisiae.
It is widely used in biomedical research as an immunostimulant and inflammatory agent, capable of activating the innate immune system and triggering a range of cellular responses.
Zymosan has been extensively studied for its role in modulating the immune response, inflammation, and host-pathogen interactions.
Researchers rely on accurate and reproducible methods to study the effects of zymosan, which is where PubCompare.ai can help.
Our platform optimizes zymosan research by leveraging AI-driven reproducibility and accuracy, helping scientists locate the best protocols and products from literature, preprints, and patents with ease.
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It is widely used in biomedical research as an immunostimulant and inflammatory agent, capable of activating the innate immune system and triggering a range of cellular responses.
Zymosan has been extensively studied for its role in modulating the immune response, inflammation, and host-pathogen interactions.
Researchers rely on accurate and reproducible methods to study the effects of zymosan, which is where PubCompare.ai can help.
Our platform optimizes zymosan research by leveraging AI-driven reproducibility and accuracy, helping scientists locate the best protocols and products from literature, preprints, and patents with ease.
Experience the future of scientific discoverye - try PubCompare.ai today!
Most cited protocols related to «Zymosan»
Cells
Common Cold
Cytokine
Edetic Acid
Flow Cytometry
Inflammation
Leukocytes
Mus
Peritoneal Fluid
Population Group
Zymosan
Antibodies
Antibody Specificity
Biological Assay
Calcium
Cell Nucleus
Cells
Cloning Vectors
Contusions
cresyl violet
Frozen Sections
Immunoglobulins
Immunoprecipitation
ITGAM protein, human
Macrophage
Macrophage-1 Antigen
Mus
Myeloid Cells
Nissl Bodies
Proteins
Protoplasm
Ribosomal RNA
Tissue, Membrane
Tissues
Tissue Stains
Western Blotting
Zymosan
Genetic Heterogeneity
Inflammation
Macrophage
Microglia
Microscopy
Necrosis
Spinal Cord
Zymosan
Animals
Connective Tissue
Decompression Sickness
Females
Freund's Adjuvant
Isoflurane
Males
Microelectrodes
Needles
Operative Surgical Procedures
Paraspinal Muscles
Rats, Sprague-Dawley
Transverse Processes
Vertebral Column
Zymosan
Luminol, Lucigenin, Isoluminol, HRP Type VI, PMA, SOD from bovine erythrocytes and zymosan were obtained from Sigma (St Louis, MO). FCS, HBSS, H2DCF-DA were from Invitrogen Molecular Probes (Oregon). DMEM was purchased from PAA (Vienna, Austria), anti-mouse antibodies CD16/CD32, CD11b-FITC, CD11c-APC, F4/80-PE-Cy5 were obtained from BD Bioscience (Mountain View, CA). Rich medium (YPD) and synthetic complete were prepared essentially as described (Kaiser et al., 1994 ). BMDM media are composed of DMEM, 10% heat-inactivated FCS, 20% l -conditioned medium. mDC media are composed of DMEM, 10% heat-inactivated FCS, 10% X-conditioned medium. C. albicans strains were grown at 30°C in YPD medium overnight, diluted to an OD600 = 0.2 the next morning, grown to the logarithmic growth phase and used for the experiment unless indicated otherwise. For the preparation of mature filaments, an overnight culture of C. albicans was diluted 1:10 in YPD + 10% FCS and grown at 37°C for 3–4 h. For experiments requiring stimulation of macrophages with filaments, an aliquot of each culture was pelleted and the dry weight was determined by routine procedures. Aliquots of cultures equalling the indicated dry weights of yeast or filaments were used for experiments. Typically, 4 × 104 yeast cells correspond to 1 μg dry weight.
Anti-Antibodies
Bos taurus
Candida albicans
Cells
Culture Media, Conditioned
Cytoskeletal Filaments
Erythrocytes
Fluorescein-5-isothiocyanate
Hemoglobin, Sickle
isoluminol
ITGAM protein, human
Lucigenin
Luminol
Macrophage
Molecular Probes
Mus
Strains
Yeasts
Zymosan
Most recents protocols related to «Zymosan»
HEK293 cells were from ATCC (CRL-1573). WT HBE cells were provided by the primary cell culture service offered from the Italian Cystic Fibrosis Research Foundation and cultured as described (46 (link)). Total lung cells, alveolar macrophages and MEFs were obtained from C57BL/6 mice as described (46 (link), 48 (link)). Zymosan from Saccharomyces cerevisiae (Sigma) was used at the final concentration of 50 µg/ml.
Cells
Cystic Fibrosis
HEK293 Cells
Lung
Macrophages, Alveolar
Mice, Inbred C57BL
Primary Cell Culture
Saccharomyces cerevisiae
Zymosan
The phagocytosis assay was performed according to the manufacturer’s instructions using pH-sensitive pHrodo Red Zymosan BioParticles (Life Technologies). The pHrodo Red conjugates do not fluoresce outside the cell at neutral pH but do fluoresce at acidic pH values such as those in phagosomes; this enables an accurate measurement of phagocytosis. Briefly, MGCs were incubated with Zymosan conjugate particles (10 μg/ml) diluted in a live-cell imaging solution (Thermo Fisher Scientific) for 2 h in the dark. Nuclei were counterstained using Hoechst (1:1,000; Life Technologies). After incubation, the cells were thoroughly washed and fixed with 2% paraformaldehyde for 10 min at room temperature. Cell fluorescence was then measured with a microplate reader (SpectraMax M5; Molecular Devices).
Acids
Biological Assay
Cell Nucleus
Cells
Fluorescence
Medical Devices
paraform
Phagocytosis
Phagosomes
Zymosan
Macrophage progenitors were cultured for 7 days in macrophage medium in a 96-well plate at a density of 1 × 105 cells/well. Mature iMΦ were incubated with or without reconstituted pHrodo Green Zymosan Bioparticles (Cat# P35365; Invitrogen, Waltham, MA, USA) at 37 °C for 2 h. Cells were harvested and analyzed by flow cytometry. Zymosan-free cells were used as negative controls to set a threshold for measuring the percentage of positive cells (Wilgenburg et al., 2013 (link)).
Flow Cytometry
Macrophage
Zymosan
Primary bone marrow-derived macrophages (BMDMs) and peritoneal macrophages (PMs) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland; 12–604 F) plus fetal bovine serum (10% v/v; Gibco, Waltham, MA, USA; 16000–044) and penicillin‒streptomycin-amphotericin B (Lonza; 17–745E). Bone marrow cells were cultured for 5–7 days in DMEM containing 25 ng/mL macrophage colony-stimulating factor (R&D Systems, Minneapolis, MN, USA; 416-ML) to generate fully differentiated BMDMs.
PMs were prepared as previously described36 (link). Briefly, 3 days after IP injection of 3% thioglycolate, mice were euthanized and IP injected with ice-cold phosphate buffered saline (PBS) containing 3% fetal bovine serum (5 mL). The wash solution was collected and centrifuged (1500 rpm, 8 min, 4 °C) to obtain a macrophage pellet. Cells were suspended in culture medium, counted, and seeded in plates for further analysis.
Before experiments, cells were incubated overnight in resting medium (DMEM with 5% fetal bovine serum). Cells were pretreated with vehicle, ATB1021, YTK-2205 or ATB1071 for 1 h and then stimulated with 100 ng/mL LPS (InvivoGen, San Diego, CA; tlrl-eblps), 20 μg/mL poly(I:C) (Sigma‒Aldrich), 10 μg/mL zymosan (Invivogen; tlrl; zyn), or 100 ng/mL Pam3CSK4 (InvivoGen; tlrl-pms) for 3, 6, or 18 h. Supernatants were collected for ELISAs; cells were lysed for RNA preparation or Western blotting. For autophagic flux analysis, bafilomycin A1 (BafA1) (Sigma‒Aldrich, b1793) was preincubated with the cells for 1.5 h at a final concentration of 100 nM.
PMs were prepared as previously described36 (link). Briefly, 3 days after IP injection of 3% thioglycolate, mice were euthanized and IP injected with ice-cold phosphate buffered saline (PBS) containing 3% fetal bovine serum (5 mL). The wash solution was collected and centrifuged (1500 rpm, 8 min, 4 °C) to obtain a macrophage pellet. Cells were suspended in culture medium, counted, and seeded in plates for further analysis.
Before experiments, cells were incubated overnight in resting medium (DMEM with 5% fetal bovine serum). Cells were pretreated with vehicle, ATB1021, YTK-2205 or ATB1071 for 1 h and then stimulated with 100 ng/mL LPS (InvivoGen, San Diego, CA; tlrl-eblps), 20 μg/mL poly(I:C) (Sigma‒Aldrich), 10 μg/mL zymosan (Invivogen; tlrl; zyn), or 100 ng/mL Pam3CSK4 (InvivoGen; tlrl-pms) for 3, 6, or 18 h. Supernatants were collected for ELISAs; cells were lysed for RNA preparation or Western blotting. For autophagic flux analysis, bafilomycin A1 (BafA1) (Sigma‒Aldrich, b1793) was preincubated with the cells for 1.5 h at a final concentration of 100 nM.
Amphotericin
Amphotericin B
Autophagy
bafilomycin A1
Bone Marrow Cells
Cells
Cold Temperature
Eagle
Enzyme-Linked Immunosorbent Assay
Fetal Bovine Serum
Injections, Intraperitoneal
Macrophage
Macrophage Colony-Stimulating Factor
Macrophages, Peritoneal
Mus
Penicillins
phenethicillin
Phosphates
Poly I-C
Saline Solution
Streptomycin
Thioglycolates
Zymosan
24 hours after polarization, hMDM phagocytosis of S. aureus and Zymosan A pHrodo Red Bioparticles (Thermo Fisher Scientific) was measured as described previously [29 (link)], with a Bioparticle incubation time of 2 hours. Fluorescence in each well was quantified using a CLARIOstar plate reader (BMG Labtech).
Fluorescence
Phagocytosis
Zymosan
Top products related to «Zymosan»
Sourced in United States, Germany, United Kingdom, Italy
Zymosan is a cell wall preparation derived from the yeast Saccharomyces cerevisiae. It is composed of a complex of carbohydrates, including β-glucans and mannans. Zymosan is commonly used as a laboratory tool to study innate immune responses and inflammation.
Sourced in United States, Germany, United Kingdom, Israel, Hungary, Macao, Japan
Zymosan A is a laboratory reagent derived from the cell wall of the yeast Saccharomyces cerevisiae. It is composed of a complex mixture of carbohydrates, proteins, and other molecules. Zymosan A is commonly used in research to stimulate immune responses and as a tool to study inflammatory processes.
Sourced in United States, France, Germany, Norway
Zymosan is a cell wall preparation derived from Saccharomyces cerevisiae (baker's yeast). It is composed of a complex mixture of polysaccharides, including β-glucans and mannans. Zymosan is commonly used as a tool for stimulating innate immune responses in vitro and in vivo.
Sourced in United States, Germany, China, United Kingdom, Sao Tome and Principe, Macao, Italy, Japan, Canada, France, Switzerland, Israel, Australia, Spain, India, Ireland, Brazil, Poland, Netherlands, Sweden, Denmark, Hungary, Austria, Mongolia
The LPS laboratory equipment is a high-precision device used for various applications in scientific research and laboratory settings. It is designed to accurately measure and monitor specific parameters essential for various experimental procedures. The core function of the LPS is to provide reliable and consistent data collection, ensuring the integrity of research results. No further details or interpretations can be provided while maintaining an unbiased and factual approach.
Sourced in United States, Germany, France, China, Switzerland, Canada, Brazil, Sao Tome and Principe, Italy, United Kingdom, Macao
Luminol is a chemiluminescent compound used in analytical chemistry and forensic science. It emits light when oxidized, which can be used to detect the presence of certain substances, such as blood. The core function of Luminol is to serve as a reagent in various chemical and biological assays.
Sourced in United States
Zymosan A is a cell wall preparation derived from the yeast Saccharomyces cerevisiae. It consists primarily of carbohydrates, including β-glucans and mannans. Zymosan A is commonly used as a research tool to study immune responses and inflammation in various in vitro and in vivo models.
Sourced in United States, France, United Kingdom, Canada, Germany, Sweden, Italy, Macao
Pam3CSK4 is a synthetic triacylated lipopeptide that mimics the structure of the acylated amino terminus of bacterial lipoproteins. It acts as a potent agonist of Toll-like receptor 2 (TLR2) and can be used in cell-based assays to study TLR2-mediated cellular responses.
Sourced in United States
PHrodo Red Zymosan Bioparticles are a fluorescent labeling reagent used to detect and measure phagocytosis in cells. They are derived from the cell wall of Saccharomyces cerevisiae and are designed to exhibit pH-dependent fluorescence, allowing for the tracking of phagosome acidification during the phagocytic process.
Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden
The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
Sourced in United States, Germany, United Kingdom, France, Italy, China, Canada, Switzerland, Sao Tome and Principe, Macao, Poland, Japan, Australia, Belgium, Hungary, Netherlands, India, Denmark, Chile
The PMA is a versatile laboratory equipment designed for precision measurement and analysis. It functions as a sensitive pressure transducer, accurately measuring and monitoring pressure levels in various applications. The PMA provides reliable and consistent data for research and testing purposes.
More about "Zymosan"
Zymosan, a complex carbohydrate derived from the cell wall of the yeast Saccharomyces cerevisiae, is a widely used immunostimulant and inflammatory agent in biomedical research.
This versatile compound has the ability to activate the innate immune system and trigger a range of cellular responses, making it a valuable tool for scientists studying host-pathogen interactions, inflammation, and immune modulation.
Researchers rely on accurate and reproducible methods to investigate the effects of zymosan.
PubCompare.ai, an innovative AI-driven platform, optimizes zymosan research by leveraging advanced techniques to ensure reproducibility and accuracy.
The platform empowers scientists to effortlessly locate the best protocols and products from literature, preprints, and patents, streamlining the discovery process.
Zymosan, also known as Zymosan A, is often compared to other immune-stimulating agents like lipopolysaccharide (LPS) and Pam3CSK4.
Luminol, a chemiluminescent compound, is commonly used to measure the respiratory burst activity induced by zymosan in phagocytic cells.
PHrodo Red Zymosan Bioparticles are another valuable tool for studying zymosan-mediated phagocytosis, while the FACSCalibur flow cytometer is often employed to analyze the cellular responses triggered by this versatile compound.
By leveraging the insights and capabilities of PubCompare.ai, researchers can navigate the wealth of information on zymosan with ease, ensuring they have access to the most reliable and effective research methods.
Experience the future of scientific discovery and explore the possibilities of zymosan research with PubCompare.ai today!
This versatile compound has the ability to activate the innate immune system and trigger a range of cellular responses, making it a valuable tool for scientists studying host-pathogen interactions, inflammation, and immune modulation.
Researchers rely on accurate and reproducible methods to investigate the effects of zymosan.
PubCompare.ai, an innovative AI-driven platform, optimizes zymosan research by leveraging advanced techniques to ensure reproducibility and accuracy.
The platform empowers scientists to effortlessly locate the best protocols and products from literature, preprints, and patents, streamlining the discovery process.
Zymosan, also known as Zymosan A, is often compared to other immune-stimulating agents like lipopolysaccharide (LPS) and Pam3CSK4.
Luminol, a chemiluminescent compound, is commonly used to measure the respiratory burst activity induced by zymosan in phagocytic cells.
PHrodo Red Zymosan Bioparticles are another valuable tool for studying zymosan-mediated phagocytosis, while the FACSCalibur flow cytometer is often employed to analyze the cellular responses triggered by this versatile compound.
By leveraging the insights and capabilities of PubCompare.ai, researchers can navigate the wealth of information on zymosan with ease, ensuring they have access to the most reliable and effective research methods.
Experience the future of scientific discovery and explore the possibilities of zymosan research with PubCompare.ai today!