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Antioxidants

Antioxidants are a class of molecules that help protect cells from damage caused by unstable molecules known as free radicals.
These compounds can neutralize free radicals, preventing them from causing oxidative stress and cellular damage.
Antioxidants play a crucial role in maintaining overall health and wellness, and have been studied for their potential benefits in a wide range of conditions, including cardiovascular disease, cancer, and neurodegenerative disorders.
Researchers can leverage the power of PubCompare.ai to optimize their antioxidants research, accessing the best protocols from literature, preprints, and patents, ensuring reproducibility and accruacy.
This innovative platform can help unleash the full potential of antioxidants research, providing researchers with the tools they need to make important discoveries.

Most cited protocols related to «Antioxidants»

The adult brain slice method we have described has been successfully implemented in a variety of experimental contexts for analysis of diverse brain regions and cell types. However, we would encourage adopters to view this method as a work in progress, and we believe there is still substantial room for systematic improvement. As a case in point, we have observed that mature adult brain slices experience high levels of oxidative stress due in large part to rapid depletion of cellular antioxidants including ascorbate and reduced glutathione (GSH). This can lead to lipid peroxidation, neuronal membrane rigidity, and tissue deterioration. There appears to be a nonuniform susceptibility to this form of oxidative damage, for example, CA1 and CA3 pyramidal neurons are particularly vulnerable, making patch clamp recording of these cells difficult in brain slices from adult and aging animals in spite of the protective recovery method.
The specific restoration of intracellular pools of neuronal GSH (e.g. supplementation with the cell-permeable GSH-ethyl ester) is highly effective at curbing deterioration and prolonging slice viability under these circumstances. Thus, we have been able to further improve the NMDG recovery method by devising strategies for stimulating de novo synthesis of glutathione during acute brain slice preparation and incubation. This is most readily accomplished by adding the inexpensive GSH precursor N-acetyl-L-cysteine (NAC, 5–12 mM) to the NMDG aCSF and HEPES holding aCSF formulas, but not the recording aCSF (seeNote 14). NAC is cell-permeable and has been shown to specifically increase neuronal glutathione levels in brain tissue (26 (link)). Within 1–2 hours of slice preparation we are able to observe notable improvements in the general appearance of neurons and in the ease of patch clamp recording, and the slices are able to be maintained in a healthy state for extended time periods.
Although these more advanced methods are not absolutely required for successful adult brain slice patch clamp recordings (as demonstrated by the specific application we have described in this chapter), we include this information in hopes of providing more options to extend the versatility of our method for particularly challenging applications. Glutathione restoration is highly effective at maintaining healthy brain slices but may not be appropriate in every experimental context, e.g. investigations focusing on oxidative stress in the aging brain. On the other hand, without implementing the NMDG protective recovery method together with glutathione restoration strategy, targeted patch clamp analysis in brain slices from very old animals is prohibitively challenging.
Publication 2014
Acetylcysteine Adult Anabolism Animals Antioxidants Brain Cells Diet, Formula Esters Gastrin-Secreting Cells Glutathione HEPES Lipid Peroxidation Muscle Rigidity Neurons Oxidative Damage Oxidative Stress Permeability Protoplasm Pyramidal Cells Reduced Glutathione Susceptibility, Disease Tissue, Membrane Tissues
The samples were classified into 24 different categories covering products from the plant kingdom, products from the animal kingdom and mixed food products. Information about sample processing (raw, cooked, dried etc), if any, was included, along with all sample specifications, i.e. product name, brand name, where the product/sample was procured and country of origin. The product information in the database was collected from the packing of the product, from supplier or purchaser. When this information was not available or the samples were handpicked, only country of origin is presented. Each sample is assigned to only one category. The classification was done according to information from the supplier or purchaser, or according to common traditional use of the food. Some foods may therefore be categorized otherwise in other food cultures. For products in the categories "Herbal/traditional plant medicine" and "Vitamin and dietary Supplements" some products may rightfully be classified as both an herbal medicine and a supplement, but are still assigned to only one category. All berries, fruits, and vegetables were fresh samples unless otherwise noted in the database. The Antioxidant Food Table contains 3139 samples. About 1300 of these samples have been published before [16 (link),17 (link),28 (link)] but for comparison and completeness we have included them in the present publication. All individual samples previously published are identified by a comment in the Antioxidant Food Table. The categories and products in the database are presented in alphabetic order. Information about brand names and product trademarks does not imply endorsement by the authors, and are reported as descriptive information for research applications only. The Antioxidant Food Table will in the future be available online as a searchable database. In addition to the products mentioned in this paper, other foods will in the future be analyzed and incorporated into the online version, which will be posted on the University of Oslo's web site.
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Publication 2010
Animals Antioxidants Berries Dietary Supplements Food Fruit Medicinal Herbs Plants Reproduction Vegetables Vitamins Volition
The RoGFP protein contains two engineered cysteine thiols, as first described by Remington et al. (RoGFP2) 11 (link). The cDNA encoding the protein was created by introducing four mutations in the mammalian GFP expression vector (pEGFP-N1) (C48S, Q80R, S147C, and Q204C) using a QuikChange Multi Site-directed mutagenesis kit (Strategene). The RoGFP construct was ligated into the VQ Ad5CMV K-NpA adenoviral shuttle vector between the KpnI and NotI sites; after sequencing and amplification this plasmid was used to generate a recombinant adenovirus to permit widespread expression in our cells (ViraQuest Inc., North Liberty, IA). The resulting redox-sensitive protein has excitation maxima at 400 and 484 nm, with emission at 525 nm. In response to changes in redox conditions, RoGFP exhibits reciprocal changes in intensity at the two excitation maxima 12 (link), and its ratiometric characteristics render it insensitive to expression levels 13 (link)-15 (link). Although RoGFP’s fluorescence behavior is relatively independent of pH and it does not respond to authentic nitric oxide (NO), reduced NADH, or the antioxidant N-acetyl-L-cysteine (NAC), its spectrum is slightly affected by reduced glutathione (GSH) possibly due to thiol-disulfide exchange (Online Figures I and II).
RoGFP was expressed in the mitochondrial matrix (Mito-RoGFP) by appending a 48 bp region encoding the mitochondrial targeting sequence from cytochrome oxidase subunit IV, at the 5′ end of the coding sequence. This construct was then ligated into the VQ Ad5CMV K-NpA plasmid between the KpnI and NotI sites, and used to generate an adenoviral vector. RoGFP was targeted to the mitochondrial inter-membrane space (IMS-RoGFP) by appending it to glycerol phosphate dehydrogenase (GPD). A cDNA construct encoding GPD, an integral protein of the inner mitochondrial membrane whose C-terminus protrudes into the inter-membrane space 17 (link), was ligated in-frame with cDNA encoding RoGFP 17 (link). The corresponding polypeptide includes amino acids 1–626 of GPD, with RoGFP at the carboxy terminus. This method has been used previously to express YFP in the inter-membrane space 18 (link). (See Online Supplemental Material for characterization of the RoGFP sensors and experimental protocols).
Publication 2009
Acetylcysteine Adenoviruses Adenovirus Vaccine Amino Acids Antioxidants Cells Cloning Vectors Cysteine Cytochrome-c Oxidase Subunit IV Disulfides DNA, Complementary Fluorescence glycerol-1-phosphate dehydrogenase Glycerol-3-Phosphate Dehydrogenase Integral Membrane Proteins Mammals Mitochondria Mitochondrial Membrane, Inner Mitochondrial Membranes Mitomycin Mutagenesis, Site-Directed Mutation NADH Open Reading Frames Oxidation-Reduction Oxide, Nitric Plasmids Polypeptides Proteins Reading Frames Reduced Glutathione Shuttle Vectors Sulfhydryl Compounds Tissue, Membrane
The free RSA of the dilute leaf extract of F. religiosa was tested using a 1,1-diphenyl-2-picryl hydrazyl (DPPH) technique. A total of 24 milligrams of DPPH were dissolved in 100 mL of methanol for making the stock solution. Filtration of DPPH stock solution using methanol yielded a usable mixture with an absorbance of around 0.973 at 517 nm. In a test tube, 3 mL DPPH workable solutions were combined with 100 µL of leaf extract. Three milliliters of solution containing DPPH in 100 µL of methanol is often given as a standard. After that, the tubes were kept in complete darkness for 30 min. The absorbance was therefore determined at 517 nm. The following formula was used to compute the percentage of antioxidants or RSA [19 (link)]:
where: Ac—Control reaction absorbance; As—Testing specimen absorbance.
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Publication 2022
Antioxidants Darkness diphenyl Filtration Methanol Plant Leaves Technique, Dilution
The overall objective of this study was to conduct a longitudinal assessment of the association of endogenous hormones with biomarkers of oxidative stress and antioxidant status during the menstrual cycle. There were four main objectives. The first was to study the intra-menstrual cycle variation of various measures of oxidative stress. This objective is intended to assess variation in several measures of oxidative stress during different phases of the menstrual cycle, including F2-8-isoprostanes in serum. Assessment of variation across individuals is planned. The second objective was to determine the relationship between specific reproductive hormone levels and oxidative stress during specific times in the menstrual cycle of premenopausal women. The panel of reproductive hormones in the blood that were of primary interest are oestradiol, progesterone, LH, FSH and sex hormone binding globulin (SHBG). The third objective was to examine the influence of external factors on both oxidative stress and hormone levels, and their interrelation. The study measured various biological factors that might influence oxidative stress, including serum concentration of certain antioxidant vitamin levels (retinoids, tocopherols, carotenoids and ascorbic acid). In addition, the study assessed other factors that might affect oxidative stress such as medication and supplement intake, cigarette smoking, alcohol consumption, dietary intake, physical activity and levels of stress. Lastly, the study was designed to evaluate the validity and reproducibility of the various biological markers included in the BioCycle study.
Publication 2009
Antioxidants Ascorbic Acid Biological Factors Biological Markers BLOOD Carotenoids Dietary Supplements Estradiol F2-Isoprostanes Hormones Menstrual Cycle Oxidative Stress Pharmaceutical Preparations Progesterone Reproduction Retinoids Serum Sex Hormone-Binding Globulin Tocopherol Vitamins Woman

Most recents protocols related to «Antioxidants»

Example 1

The target organoselenium compound 5 is synthesized using the Ugi four components reaction. The synthesis starts by the reaction of quinazoline-2-carbaldehyde (1) (1 mmol) with 4-(methylselanyl)aniline (2) (1 mmol) followed by the addition of 2-((3-methyl-1,4-dioxo-1,4-dihydronaphthalen-2-yl)thio)acetic acid (3) (1 mmol) and 2-isocyano-2-methylpropane (4) (1.2 mmol). The reaction proceeds smoothly at room temperature in methanol as solvent.

[Figure (not displayed)]

It is to be understood that the organic selenide compounds and the use thereof with DPPD are not limited to the specific embodiments described above, but encompasses any and all embodiments within the scope of the generic language of the following claims enabled by the embodiments described herein, or otherwise shown in the drawings or described above in terms sufficient to enable one of ordinary skill in the art to make and use the claimed subject matter.

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Patent 2024
4-phenylenediamine Acetic Acid Anabolism aniline Antioxidants diphenyl Gene, BRCA1 Generic Drugs Methanol N,N'-diphenyl-4-phenylenediamine Organic Chemicals Organoselenium Compounds Quinazolines Solvents Therapeutics

EXAMPLE 5

The antioxidant potential of Extracts 1-3 and 6-9 was analyzed using a kit by Oxford Biomedical Research, P.O. Box 522, Oxford MI 48371. This colorimetric microplate assay allows comparison of each Extract 1-3 and 6-9 to a standard to determine the total copper reducing equivalents. Generally the assay was performed by preparing the standards, and allowing dilution buffer, copper solution and stop solution to equilibrate to room temperature for about 30 minutes prior to running the assay. Both Extracts 1-3 and 6-9 samples and standards were diluted 1:40 in the provided dilution buffer (e.g. 15 mL serum+585 mL buffer). Next, 200 mL of diluted Extract samples or standards were placed in each well. The plate was read at 490 nanometers (nm) for a reference measurement. Then 50 mL of Cu++ solution was added to each well and incubated about 3 minutes at room temperature. 50 mL of stop solution was added and the plate read a second time at 490 nm.

The data in Table 15 demonstrates the antioxidant potential of each of Extracts 1-3 and 6-9 at two different concentrations. The data further explains the effectiveness of extracts against damaging oxidant or ROS events (above discussed) generated during in vitro processing of reproductive cells.

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Patent 2024
Antioxidants Biological Assay Buffers Cells Colorimetry Copper Genitalia Germ Cells Oxidants Serum Technique, Dilution

Example 4

Anti-oxidant activity

The prepared novel complex showed Antioxidant activity with an IC50 of 9.7 μg/ml against the breast cancer cell line compared with the 1-ascorbic acid standard antioxidant (IC50=55.2 μg/ml).

It is to be understood that the Schiff base-metal complex is not limited to the specific embodiments described above, but encompasses any and all embodiments within the scope of the generic language of the following claims enabled by the embodiments described herein, or otherwise shown in the drawings or described above in terms sufficient to enable one of ordinary skill in the art to make and use the claimed subject matter.

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Patent 2024
Antioxidant Activity Antioxidants Ascorbic Acid Coordination Complexes Generic Drugs Imines MCF-7 Cells Pharmaceutical Preparations prisma Schiff Bases Tetragonopterus

Example 2

In an exemplary embodiment, a capsule is provided containing a fill material comprising:

TABLE 3
Ingredient%mg/CapsuleFunction
Ultra-micronized30.77200.00Active
Progesterone
Medium Chain65.93428.55Solubilizing Agent
Triglyceride
(MIGLYOL 812 or
equivalent)
Lauroyl polyoxyl-32-3.0019.50Suspending Agent
glycerides
(GELUCIRE 44/14 or
equivalent)
Butylated0.031.95Antioxidant
Hydroxytoluene
Total100650

In various embodiments, amounts of MIGLYOL may be present in a range from about 35-95% by weight; GELUCIRE 44/14 from about 0.5-30% by weight; and BHT from about 0.01-0.1% by weight.

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Patent 2024
Antioxidants Capsule gelucire 44-14 Glycerides miglyol 812 Progesterone Suspending Agents Triglycerides

Example 14

Variables tested include: concentration of HA, concentration of zinc oxide, concentration of titanium dioxide, addition of vitamin C, and serum preparation method.

FIGS. 94A-94C are tables summarizing embodiments of cosmetic serums of the present disclosure with varying additives and concentrations of components suitable for protection against ultraviolet radiation (UV). Table 33 provides an embodiment of a hydrating serum of the present disclosure with vitamin C.

TABLE 33
Embodiment of Hydrating serum of
the present disclosure with vitamin C
% Silk Solution  1.0% w/v
(60 minute boil, 25 kDA)
Hyaluronic Acid 0.75% w/v
(sodium hyaluronate)
Lemongrass Oil20 uL/15 mL
silk solution
Sodium Ascorbyl Phosphate  6 g
Lactic Acid1.2 mL

A serum of the present disclosure can be made with from about 0.25% to about 10% sodium hyaluronate (increasing % results in more viscous serum). 0.5% to about 10% silk solutions can be used to prepare a serum of the present disclosure. A serum of the present disclosure can be clear and have a yellow tinted color. A serum of the present disclosure can have a pH=6. A serum of the present disclosure can have a lubricious texture that is rubbed in easily without residue.

Concentration of HA:

Hyaluronic acid (Sodium Hyaluronate) was tested as an ingredient in the UV silk serum due to its hygroscopic properties and widely accepted use in cosmetic products to promote hydration of skin. 1%, 2.5% and 5% HA solutions were tested. With increasing HA %, the serum became more viscous and gel like. 1% HA was not feasible for the UV serum due to the fact that the UV additives (zinc oxide, titanium dioxide) are not water soluble and need to be dispersed. 1% HA was not viscous enough for dispersion and the UV additives precipitated out. 2.5% gave the best consistency based on preferred feel, texture and viscosity and was able to disperse the UV additives. 5% was a very thick, viscous serum.

Concentration of Mineral Filters: Zinc Oxide and Titanium Dioxide:

Zinc oxide and titanium dioxide were explored as UV additives that are considered safe. These additives mechanically protect from UV radiation by forming a physical reflective barrier on the skin. Both are not soluble in water and must be dispersed for the current aqueous solution. Zinc oxide concentration varied from 2.5%, 3.75%, 5%, 5.625%, 10%, 12% and 15%. Titanium dioxide concentrations varied from 1.25%, 1.875%, 3%, 5% and 10%. Increasing the concentration of UV additives resulted in minor increases of white residue and how well dispersed the additives were, however if mixed well enough the effects were negligible. Zinc oxide and titanium dioxide were mixed together into serums in order to achieve broad spectrum protection. Zinc oxide is a broad spectrum UV additive capable of protecting against long and short UV A and UV B rays. However titanium dioxide is better at UV B protection and often added with zinc oxides for best broad spectrum protection. Combinations included 3.75%/1.25% ZnO/TiO2, 5.625%/1.875% ZnO/TiO2, 12%/3% ZnO/TiO2, 15%/5% ZnO/TiO2. The 3.75%/1.25% ZnO/TiO2 resulted in spf 5 and the 5.625%/1.875% ZnO/TiO2 produced spf 8.

Vitamin C:

Sodium ascorbyl phosphate was used as a vitamin C source. Formulations were created with the vitamin C concentration equal to that in the silk gel (0.67%). Formulations were also created with 20% sodium ascorbyl phosphate which is soluble in water.

Serum Preparation:

The vitamin C (sodium ascorbyl phosphate) must first be dissolved in water. Sodium hyaluronate is then added to the water, mixed vigorously and left to fully dissolve. The result is a viscous liquid (depending on HA %). The viscosity of the HA solution allows even dispersion of the zinc oxide and titanium dioxide and therefore HA must be mixed before addition of UV additives. The zinc oxide and titanium dioxide are then added to the solution and mixed vigorously with the use of an electric blender. Silk solution is then added and mixed to complete the serum formulation.

Chemical Filters:

A UV serum of the present disclosure can include one, or a combination of two or more, of these active chemical filter ingredients: oxybenzone, avobenzone, octisalate, octocrylene, homosalate and octinoxate. A UV serum of the present disclosure can also include a combination of zinc oxide with chemical filters.

In an embodiment, a UV serum of the present disclosure can be applied approximately 15 minutes before sun exposure to all skin exposed to sun, and can be reapplied at least every 2 hours. In an embodiment, a UV serum of the present disclosure includes water, zinc oxide, sodium hyaluronate, titanium dioxide, silk, and vitamin C or a vitamin C derivative such as sodium ascorbyl phosphate. In an embodiment, a UV serum of the present disclosure protects skin and seals in moisture with the power of silk protein. In an embodiment, a UV serum of the present disclosure improves skin tone, promotes collagen production and diminishes the appearance of wrinkles and fine lines with the antioxidant abilities of vitamin C. In an embodiment, a UV serum of the present disclosure delivers moisture for immediate and long-term hydration throughout the day with concentrated hyaluronic acid. In an embodiment, a UV serum of the present disclosure helps prevent sunburn with the combined action of zinc oxide and titanium dioxide. In an embodiment, a UV serum of the present disclosure is designed to protect, hydrate, and diminish fine lines while shielding skin from harsh UVA and UVB rays. In an embodiment, the silk protein in a UV serum of the present disclosure stabilizes and protects skin while sealing in moisture, without the use of harsh chemical preservatives or synthetic additives. In an embodiment, the vitamin C/derivative in a UV serum of the present disclosure acts as a powerful antioxidant that supports skin rejuvenation. In an embodiment, the sodium hyaluronate in a UV serum of the present disclosure nourishes the skin and delivers moisture for long-lasting hydration. In an embodiment, the zinc oxide and titanium dioxide in a UV serum of the present disclosure shields skin from harmful UVA and UVB rays. The silk protein stabilization matrix in a UV serum of the present disclosure protects the active ingredients from the air, to deliver their full benefits without the use of harsh chemicals or preservatives. The silk matrix also traps moisture within the skin furthering the hydrating effect of the sodium hyaluronate.

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Patent 2024
Acids Antioxidants Ascorbic Acid avobenzone Collagen Electricity Feelings Figs Furuncles homosalate Hyaluronic acid Minerals octinoxate octisalate octocrylene oxybenzone Pharmaceutical Preservatives Proteins Radiation Rejuvenation SERPINA3 protein, human Serum Serum Proteins Silk Skin Skin Pigmentation sodium ascorbyl phosphate Sodium Hyaluronate Strains Sunburn titanium dioxide Viscosity Vitamin A Vitamins west indian lemongrass oil Zinc Oxide

Top products related to «Antioxidants»

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Trolox is a water-soluble vitamin E analog that functions as an antioxidant. It is commonly used in research applications as a reference standard for measuring antioxidant capacity.
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DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.
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Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
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The Antioxidant Assay Kit is a laboratory instrument designed to measure the antioxidant capacity of biological samples. It provides a quantitative assessment of the ability of the sample to neutralize free radicals and reactive oxygen species.
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ABTS is a laboratory reagent used for the detection and quantification of peroxidase activity. It is a colorimetric substrate that undergoes a color change when oxidized by peroxidases, allowing for spectrophotometric or colorimetric analysis.
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
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NAC is a laboratory instrument used for the analysis and quantification of various analytes in samples. It functions by leveraging advanced spectroscopic techniques to detect and measure the presence and concentration of specific chemical compounds or biological molecules. The core purpose of NAC is to provide accurate and reliable data to support scientific research, clinical diagnostics, and other analytical applications.
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Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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The Multiskan GO is a microplate spectrophotometer designed for a wide range of absorbance-based applications. It provides reliable and accurate measurements across a broad wavelength range.

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