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Floxuridine

Floxuridine, a potent antineoplastic agent, is widely used in the treatment of various cancers.
It works by inhibiting DNA synthesis, leading to cell death and growth suppression.
Floxuridine has demonstrated efficacy in the management of solid tumors, including colorectal, gastric, and pancreatic cancers.
Reasearch protocols and product comparisons are crucial for optimzing Floxuridine therapies and improving patient outcomes.
PubCompare.ai leverages AI-powered analysis to help researchers effortlessly identify the most effective Floxuridine procedures and procedures, streamlining the research process and accelerating advancements in this important area of oncology.

Most cited protocols related to «Floxuridine»

1
Cultivation of Thalassiosira pseudonana in Artificial Seawater
Cited 44 times
Parasites were grown in hTERT-BJ1 (clontech) cells in supplemented Dulbecco's modified Eagle's medium [67] ). Parasite cloning and plaque assays were performed in human foreskin fibroblasts (HFF). For the selection of stable transgenic lines, drugs were added as follow: 1 µM pyrimethamine added one day after transfection for one week, 20 µM chloramphenicol added the day of transfection for three weeks, 5 µM FUDR added two days after transfection for one week. To repress the regulated promoter, parasites were grown in the presence of 0.5 µM anhydrotetracycline (ATc).
Thalassiosira pseudonana (Hustedt) Hasle et Heimdal CCMP1335 was grown in an artificial seawater medium (EASW) according to the North East Pacific Culture Collection protocol (http://www3.botany.ubc.ca/cccm/NEPCC/esaw.html) at 18°C under constant light. Where indicated, NaNO3 was omitted from the medium (nitrogen-free medium) or replaced by 0.55 mM NH4Cl (ammonium medium).
Sheiner L., Demerly J.L., Poulsen N., Beatty W.L., Lucas O., Behnke M.S., White M.W, & Striepen B. (2011). A Systematic Screen to Discover and Analyze Apicoplast Proteins Identifies a Conserved and Essential Protein Import Factor. PLoS Pathogens, 7(12), e1002392.
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Publication 2011
Ammonium anhydrotetracycline Animals, Transgenic Biological Assay Cells Chloramphenicol Fibroblasts Floxuridine Foreskin Homo sapiens Light Nitrogen Parasites Pharmaceutical Preparations Pyrimethamine Senile Plaques Transfection
2
CRISPR-Cas9 Screen in Toxoplasma
Cited 23 times

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Publication 2016
Biopharmaceuticals Blood Cells DNA Library DNA Replication Elp1 protein, human Filtration Floxuridine Infection Nested Polymerase Chain Reaction Oligonucleotide Primers Parasites Pyrimethamine Substance Abuse Detection Tissues Transfection
3
Culturing Embryonic Rat Cortical Neurons
Cited 8 times
Cortical neurons were harvested from embryonic day-18 Wistar rats (either sex, Charles River Laboratories, Wilmington, MA). Cultures were prepared according to a previously described procedure with some modifications (Ueno et al., 2012 (link)). Briefly, embryos were removed, and the cerebral cortex dissected, stripped of meninges, and dissociated by a combination of Ca2+- and Mg2+- free Hanks balance salt solution (HBSS) containing 0.125% trypsin digestion for 15 min, then mechanically triturated for ~20 times. The triturated cells were passed through a 40 μm cell strainer and counted to obtain a concentration of 3×107cells/ml.
To separate axons from neuronal soma, a microfluidic chamber (Standard Neuron Device, Cat# SND450, Xona Microfluidics, Temecula, CA) was employed (Taylor et al., 2005 (link); Taylor et al., 2009 (link)). The small dimension of the microgrooves in the chamber allows axons to sprout from the cells seeded compartment into the other compartment of the chamber, but prevents the passage of cell bodies(Taylor et al., 2005 (link); Taylor et al., 2009 (link)). Briefly, cleaned, sterilized, and dried chambers were affixed to poly-D-lysine (PDL) (Sigma-Aldrich, CA) -coated dishes (35mm, Corning). The cortical neurons were plated at a density of 6×105cells/chamber in DMEM with 5% FBS and, incubated for an initial 24 h. After 24 h, cell culture was initiated with the addition of neurobasal growth medium (Invitrogen), 2% B-27 (GIBCO), 2mM GlutaMax, and 1% antibiotic-antimycotic. On day in vitro (DIV) 3, one-half of the medium was replaced with culture medium containing 20 μM 5-fluorodeoxyuridine. The growth media was changed every other day thereafter.
Zhang Y., Ueno Y., Liu X.S., Buller B., Wang X., Chopp M, & Zhang Z.G. (2013). The MicroRNA-17–92 Cluster Enhances Axonal Outgrowth in Embryonic Cortical Neurons. The Journal of Neuroscience, 33(16), 6885-6894.
Publication 2013
Antibiotics Axon Carisoprodol Cell Body Cell Culture Techniques Cells Cortex, Cerebral Culture Media Digestion Embryo Floxuridine Hyperostosis, Diffuse Idiopathic Skeletal Lysine Medical Devices Meninges Neurons Poly A Rats, Wistar Rivers Sodium Chloride Trypsin
4
C. elegans Pathogen Avoidance Assay
Cited 8 times
C. elegans were cultivated and strains constructed using standard methods27 (link). Transgenic strains were generated using indicated constructs and standard microinjection methods28 (link). MosSCI single-copy insertions were generated as described29 (link). Assays measuring C. elegans pathogen avoidance behaviour utilized P. aeruginosa PA14 plates prepared as follows: a 100 mL solution of LB was inoculated with a single colony of P. aeruginosa PA14 and grown overnight without shaking at 37°C (OD=0.2-0.3). 30 μL of this culture was used to seed the center of each 100-mm NGM plate, and the seeded plates were incubated for 24 h at room temperature (22.5°C). Approximately 30 Larval Stage 4 (L4) animals were transferred onto plates containing the P. aeruginosa PA14 lawn at 22.5°C, and occupancy was determined at the indicated times. Survival assays were carried out on 35 mm Slow-Killing Assay plates30 (link) supplemented with 5-fluorodeoxyuridine (0.05 mg/mL) and seeded with P. aeruginosa PA14 prepared as above and maintained at 22.5°C. Laser ablations were performed on L3-stage larvae as described in text. Microscopy and image analysis was carried out on an AxioImager Z1 fluorescence microscope fitted with CCD camera (AxioCam) and processed with Axioplan image processor software (Zeiss). The statistical analyses were performed using GraphPad Prism software.
Chang H.C., Paek J, & Kim D.H. (2011). Natural Polymorphisms in C. elegans HECW-1 E3 Ligase Affect Pathogen Avoidance Behaviour. Nature, 480(7378), 525-529.
Publication 2011
Animals Animals, Transgenic Biological Assay Caenorhabditis elegans Floxuridine Insertion Mutation Larva Laser Ablation LB-100 Microinjections Microscopy Microscopy, Fluorescence Pathogenicity prisma PRO 140 Pseudomonas aeruginosa Strains
5
Modified Dietary Restriction Assay for E. coli
Cited 7 times
The msDR method was modified from previously described [49] (link). Overnight culture of E. coli OP50 grown at 37°C was centrifuged at 3,000 rpm for 30 minutes to collect bacteria cells. The bacterial pellet was washed with the S buffer, and the bacterial concentration was adjusted to 1.0×1012 cfu/ml. Serial dilutions were performed to achieve bacterial concentrations of 1.0×1011, 1.0×1010, 1.0×109, and 1.0×108 cfu/ml. Diluted bacterial cultures were spotted onto DR agar plates, which were modified from the standard nematode growth media (NGM) plates by excluding peptone and increasing agar from 1.7% to 2.0%. Carbenicillin (50 µg/ml) was added to the agar plates to further prevent bacteria growth. Synchronized L4 larvae growing under standard lab conditions (NGM plates with OP50 food, 20°C) were transferred to fresh NGM plates with OP50 food and 5 µg/ml of FUdR, and were incubated at 25°C overnight. Day 1 adult animals were then transferred to DR agar plates seeded with OP50 at different concentrations.
In the first week of lifespan experiments and heat stress assays, 5-fluorodeoxyuridine (FUdR) at 50 µg/ml was also added into the agar plates to prevent progeny from hatching.
Chen D., Thomas E.L, & Kapahi P. (2009). HIF-1 Modulates Dietary Restriction-Mediated Lifespan Extension via IRE-1 in Caenorhabditis elegans. PLoS Genetics, 5(5), e1000486.
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Publication 2009
Adult Agar Animals Bacteria Biological Assay Buffers Carbenicillin Cells Culture Media Escherichia coli Floxuridine Food Heat Stress Disorders Larva Nematoda Peptones Technique, Dilution

Most recents protocols related to «Floxuridine»

1
Anaerobic Cultivation of Bacteroides Strains
Bacteroides strains were grown on supplemented brain heart infusion medium (BHIS) plates or liquid cultures supplemented with 1mg/ml of vitamin K3 (Acros Organics/Fisher Scientific, Geel, Belgium) when not specified (Basic 2008)(Lim, 2017) Bacteroides strains were incubated under anaerobic conditions (10% CO2, 10% H2, 80% N2) within a 37°C Whitley A55 anaerobic chamber (Don Whitley Scientific, Victoria Works, UK). All BHIS plates contain 60 mg/ml of gentamycin sulfate when not specified. E. coli was grown in Luria broth (LB) for liquid or solid agar culture and incubated at 37°C. Antibiotics were added to media as needed: carbenicillin 150 mg/ml, erythromycin 12.5 mg/ml, tetracycline 6 mg/ml, and 2-Deoxy-5-fluorouridine (FudR) 200 mg/ml.
Robitaille S., Simmons E.L., Verster A.J., McClure E.A., Royce D.B., Trus E., Swartz K., Schultz D., Nadell C.D, & Ross B.D. (2023). Community composition and the environment modulate the population dynamics of type VI secretion in human gut bacteria. bioRxiv.
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Publication Preprint 2023
Agar Antibiotics, Antitubercular Bacteroides Brain Carbenicillin Erythromycin Escherichia coli Floxuridine Heart Strains Sulfate, Gentamicin Tetracycline Vitamin K3
2
Evaluating Pharmacological Inhibitors
NVP-2, Nutlin-3a, 5-fluorouridine, 5-fluorodeoxyuridine, THZ531 and Pictilisib were from MedChemExpress. 5-fluorouracil, Senexin A, OTS964 and Triptolide were from Selleck Chemicals. YLK-5–124 and THAL-SNS-032 were a gift from Nathanael S. Gray’s Laboratory (Stanford University, USA). iCDK9 was a gift from Qiang Zhou’s Laboratory (University of California, Berkley, USA). PP2A activator DT-061 was a gift from Jukka Westermarck’s Laboratory (University of Turku, Finland).
Wang Z., Mačáková M., Bugai A., Kuznetsov S.G., Hassinen A., Lenasi T., Potdar S., Friedel C.C, & Barborič M. (2023). P-TEFb promotes cell survival upon p53 activation by suppressing intrinsic apoptosis pathway. Nucleic Acids Research, 51(4), 1687-1706.
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Publication 2023
5-fluorouridine Floxuridine Fluorouracil nutlin-3A OTS964 pictilisib PPP2R4 protein, human SNS-032 Thalidomide THZ531 triptolide
3
Generation of Complemented T. gondii Atg8 Cell Lines
Tachyzoites of the T. gondii cKD TgAtg8 cell line (12 (link)), as well as derived transgenic parasites generated in this study, were maintained by serial passage in a human foreskin fibroblast (HFF; American Type Culture Collection; CRL 1634) cell monolayer grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), supplemented with 5% decomplemented fetal bovine serum, 2-mM l-glutamine, and a cocktail of penicillin-streptomycin at 100 μg/mL.
Complemented cell lines were generated by insertion of an additional TgAtg8 copy at the uracil phosphoribosyltransferase (UPRT) locus in the cKD TgAtg8 mutant (12 (link)). The pGFP-TgAtg8 plasmid (64 (link)) was used as a template with primers ML2463 and ML2464, and the products were self-ligated to generate p-GFP-TgAtg8Δ68-76 excluding amino acids 68 to 76 (QCAQNSGLP). A 1.5-kbp sequence corresponding to the promoter region of TgAtg8 was obtained by PCR from genomic DNA with primers ML2429 and ML2430 and cloned with NsiI upstream of the GFP-TgAtg8 or GFP-TgAtg8ΔLoop fragment in its respective plasmid. These were then used as a PCR template to amplify, with primers ML2624 and ML2625, a cassette containing the TgAtg8 promoter followed by the sequence coding for GFP-fused wild-type (WT) or truncated TgAtg8. These cassettes were cloned using NotI and XmaI into the pUPRT-TUB-Ty plasmid (65 (link)) to yield the pUPRT-GFP-TgAtg8 and pUPRT-GFP-TgAtg8ΔLoop plasmids, respectively. These plasmids were then linearized with KpnI and BamHI prior to transfection into the cKD TgAtg8 cell line (12 (link)) together with a plasmid expressing Cas9 and a UPRT-specific guide RNA under the control of a U6 promoter (66 (link)). Then transgenic parasites were selected with 5 μM fluorodeoxyuridine and cloned by limiting dilution. Primers are listed in Table S1.
Walczak M., Meister T.R., Nguyen H.M., Zhu Y., Besteiro S, & Yeh E. (2023). Structure-Function Relationship for a Divergent Atg8 Protein Required for a Nonautophagic Function in Apicomplexan Parasites. mBio, 14(1), e03642-21.
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Publication 2023
Amino Acids Animals, Transgenic Cell Lines Cells DNA Primers Eagle Fetal Bovine Serum Fibroblasts Floxuridine Foreskin Genome Glutamine Homo sapiens Oligonucleotide Primers Open Reading Frames Parasites Penicillins Plasmids Streptomycin Technique, Dilution Transfection U6 small nuclear RNA uracil phosphoribosyltransferase
4
IMC29 Knockout and Mutant Generation
The full coding region of IMC29 was PCR amplified from cDNA using primers P21/P22 and cloned into a UPRT-locus knockout vector (10 (link)) using BglII/NotI (all enzymes purchased from NEB). The endogenous promoter was amplified from genomic DNA using primers P23/P24 and inserted with NsiI/BglII upstream of the coding sequence, resulting in UPRTKO-IMC29pro-IMC29FL. This complement vector was then linearized with DraIII-HF and transfected into Δimc29PC parasites along with a pU6 that targets the UPRT coding region. Selection was performed with 5 μg/mL 5-fluorodeoxyuridine (FUDR) for replacement of UPRT (57 (link)). Potential clones were screened by IFA, and an HA-positive clone was designated IMC29FL.
For IMC29 deletion constructs, UPRTKO-IMC29pro-IMC29FL was used as the template to amplify truncations from the IMC29 coding region, using primers P25 to P31. The P22 reverse primer was utilized to amplify each N-terminal truncation. The P21 forward primer was utilized to amplify the two C-terminal truncations. Each insert was cloned into a BglII/NotI-digested UPRTKO-IMC29pro-IMC29FL. For the additional C-terminal truncation (Δ1111-1218), the UPRTKO-IMC29pro-IMC29FL plasmid was used as the template using the Q5 mutagenesis kit. Primers P36 and P37 were used for inverse PCR to amplify the entire plasmid except for residues 1111 to 1218. For the phosphorylation mutant construct, phosphorylation sites were annotated from ToxoDB, combining phosphoproteomic data of both TgME49_243200 and TgGT1_243200. These include T42, S47, S50, S52, S68, S69, S73, T80, S205, S206, S526, S528, S736, T778, T779, T795, S797, T811, S813, S815, S817, S844, S846, T977, S1087, T1090, T1097, Y1100, S1105, S1106, T1220, and T1230. The mutated residues were designed in three synthetic gene blocks and ligated together using BglII/SgrAI, SgrAI/BamHI, and BamHI/NotI to generate a full-length IMC29 with all 32 residues mutated simultaneously to alanine. The same processes for linearization, transfection, and selection were followed for all deletion and mutant constructs.
Back P.S., Moon A.S., Pasquarelli R.R., Bell H.N., Torres J.A., Chen A.L., Sha J., Vashisht A.A., Wohlschlegel J.A, & Bradley P.J. (2023). IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome. mBio, 14(1), e03042-22.
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Publication 2023
Alanine Cloning Vectors Deletion Mutation DNA, Complementary Enzymes Floxuridine Genome Inverse PCR Mutagenesis Oligonucleotide Primers Parasites Phosphorylation Plasmids Synthetic Genes T1220 Transfection
5
Propagation of T. gondii RHΔhxgprt Strain
Parental T. gondii RHΔhxgprt (wild-type) and subsequent strains were grown on confluent monolayers of human foreskin fibroblasts (HFF) (BJ, ATCC, Manassas, VA) at 37°C and 5% CO2 in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% fetal bovine serum (Gibco), 5% Cosmic calf serum (HyClone), and 1× penicillin-streptomycin-l-glutamine (Gibco). Constructs containing selectable markers were selected using 1 μM pyrimethamine (dihydrofolate reductase-thymidylate synthase [DHFR-TS]), 50 μg/mL mycophenolic acid-xanthine (HXGPRT), or 40 μM chloramphenicol (CAT) (54 (link)– (link)56 (link)). Homologous recombination to the UPRT locus was negatively selected using 5 μM 5-fluorodeoxyuridine (FUDR) (57 (link)).
Back P.S., Moon A.S., Pasquarelli R.R., Bell H.N., Torres J.A., Chen A.L., Sha J., Vashisht A.A., Wohlschlegel J.A, & Bradley P.J. (2023). IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome. mBio, 14(1), e03042-22.
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Publication 2023
Chloramphenicol Cosmic composite resin Eagle Fetal Bovine Serum Fibroblasts Floxuridine Foreskin Glutamine Homologous Recombination Homo sapiens Mycophenolic Acid Parent Penicillins Pyrimethamine Serum Strains Streptomycin Tetrahydrofolate Dehydrogenase Thymidylate Synthase Xanthines

Top products related to «Floxuridine»

1
Fluorodeoxyuridine by Merck Group
Sourced in United States, Spain, Italy
Fluorodeoxyuridine is a laboratory reagent used in various research applications. It is a synthetic analog of the nucleoside thymidine, which is a component of DNA. Fluorodeoxyuridine can be used to study cellular processes and DNA metabolism in biological systems.
2
Glutamax by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, France, Switzerland, Canada, Japan, Australia, China, Belgium, Italy, Denmark, Spain, Austria, Netherlands, Sweden, Ireland, New Zealand, Israel, Gabon, India, Poland, Argentina, Macao, Finland, Hungary, Brazil, Slovenia, Sao Tome and Principe, Singapore, Holy See (Vatican City State)
GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
3
B27 supplement by Thermo Fisher Scientific
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B27 supplement is a serum-free and animal component-free cell culture supplement developed by Thermo Fisher Scientific. It is designed to promote the growth and survival of diverse cell types, including neurons, embryonic stem cells, and other sensitive cell lines. The core function of B27 supplement is to provide a defined, optimized combination of vitamins, antioxidants, and other essential components to support cell culture applications.
4
5 fluorodeoxyuridine fudr by Merck Group
Sourced in United States
5-fluorodeoxyuridine (FUdR) is a laboratory reagent used in scientific research. It is a synthetic analogue of the nucleoside deoxyuridine. FUdR functions as an inhibitor of the enzyme thymidylate synthase, which is involved in DNA synthesis.
5
Uridine by Merck Group
Sourced in United States, Germany, China, United Kingdom, France, Japan, Sao Tome and Principe
Uridine is a nucleoside that is a constituent of ribonucleic acid (RNA). It serves as a precursor in the biosynthesis of uridine triphosphate (UTP) and other pyrimidine nucleotides. Uridine can be used as a research tool in various biological and biochemical applications.
6
Papain by Worthington
Sourced in United States, Germany, Japan, Austria
Papain is a proteolytic enzyme derived from the fruit of the papaya plant. It is a white to off-white powder with a neutral pH. Papain functions as a catalyst in the breakdown of proteins.
7
5 fluorodeoxyuridine by Merck Group
Sourced in Germany
5-fluorodeoxyuridine is a synthetic nucleoside analogue. It is commonly used as a research tool in cellular and molecular biology laboratories.
8
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
9
Neurobasal a medium by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Italy, Japan, Canada, Switzerland, Morocco, Belgium
Neurobasal-A medium is a cell culture medium designed for the maintenance and growth of neuronal cell types. It provides a defined, serum-free formulation that supports the survival and differentiation of neurons in vitro. The medium is optimized to support the specific nutritional requirements of neuronal cells.
10
Matrigel by BD
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.

More about "Floxuridine"

Floxuridine, also known as Fluorodeoxyuridine (FUDR) or 5-fluorodeoxyuridine, is a potent antineoplastic agent that has been widely used in the treatment of various types of cancer, including colorectal, gastric, and pancreatic.
This pyrimidine analog works by inhibiting DNA synthesis, leading to cell death and growth suppression.
Floxuridine has demonstrated efficacy in the management of solid tumors, and research protocols and product comparisons are crucial for optimizing its therapies and improving patient outcomes.
PubCompare.ai, an AI-powered platform, can help researchers effortlessly identify the most effective Floxuridine procedures and products, streamlining the research process and accelerating advancements in this important area of oncology.
In addition to Floxuridine, other related terms and substances that are relevant in this context include Fluorodeoxyuridine (FUdR), Uridine, Papain, GlutaMAX, B27 supplement, FBS, and Neurobasal-A medium.
These components may be used in research protocols or as part of cell culture and tissue engineering techniques to support or complement the use of Floxuridine.
Optimizing Floxuridine therapies requires a comprehensive understanding of these related terms and substances, as well as the latest research protocols and product comparisons.
By leveraging the power of AI-driven analysis, researchers can streamline their workflow, identify the most effective approaches, and drive advancements in the treatment of cancer using this important antineoplastic agent.