Single-cell suspensions were prepared from the liver, spleen, thymus, bone marrow, lymph nodes, and intestinal intraepithelial lymphocytes (IELs). Liver was perfused with PBS via the portal vein until the liver was opaque and pressed through a 70-μm cell strainer (Becton Dickinson). Total liver cells were then resuspended in a 40% isotonic Percoll solution (Amersham Pharmacia Biotech) underlaid with a 60% isotonic Percoll solution. After centrifugation for 20 min at 900 g, mononuclear cells were isolated at the 40/60% interface. The cells were washed once with RPMI 1640 medium (Life Technologies) supplemented with 5% FBS (HyClone). For the spleen, lymphocytes were isolated by separation of total spleen cells on a lympholyte gradient (Cedarlane Labs.). Bone marrow cells (femur, tibia) were depleted of B cells using magnetic separation. CD19 microbeads (Miltenyi Biotec) were incubated with bone marrow cells, washed, and run over a column as per the manufacturer's protocol. To isolate IELs, the small intestine was opened longitudinally and flushed of fecal content. The intestine was then cut into 0.5-cm pieces, transferred into 250-ml Erlenmeyer flasks, and shaken three times at 200 rpm for 30 min, each time at 37°C, in HBSS without Ca2+ or Mg2+ and containing 1 mM dithiothreitol (Sigma-Aldrich). The cell suspensions were passed through a 60-μm nylon mesh, and cells were pelleted by centrifugation at 1,200 rpm. The cell pellets were resuspended in 40% Percoll, layered over 70% Percoll, and centrifuged at 900 g for 20 min. Cells from the interface were collected and washed once before analysis.
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Isotonic Solutions
Isotonic Solutions
Isotonic solutions are aqueous mixtures with the same osmotic pressure as body fluids, allowing for the maintenance of normal physiological conditions.
These solutions are commonly used in medical and research settings to maintain cell viability and prevent osmotic stress.
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These solutions are commonly used in medical and research settings to maintain cell viability and prevent osmotic stress.
PubCompare.ai's AI-driven platform helps researchers optimize their protocols and enhance reproducibility by easily locating the best isotonic solution protocols from literature, preprints, and patents using sophisticated comparisons.
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Most cited protocols related to «Isotonic Solutions»
B-Lymphocytes
Bone Marrow
Bone Marrow Cells
Cells
Cell Separation
Centrifugation
Dithiothreitol
Feces
Femur
Hemoglobin, Sickle
Hepatic Vein
Hepatocyte
Intestines
Intestines, Small
Intraepithelial Lymphocytes
Isotonic Solutions
Liver
Lymphocyte
Microspheres
Nodes, Lymph
Nylons
Pellets, Drug
Percoll
Spleen
Thymus Gland
Tibia
Patch-clamp experiments using mitoplasts were performed as described previously [18] (link), [19] (link). Briefly, mitoplasts were prepared from a sample of human astrocytoma mitochondria placed in a hypotonic solution (5 mM HEPES, 200 µM CaCl2, pH = 7.2) for approximately 1 min to induce swelling and breakage of the outer membrane. Then, a hypertonic solution (750 mM KCl, 30 mM HEPES, 200 µM CaCl2, pH = 7.2) was added to restore the isotonicity of the medium. The patch-clamp pipette was filled with an isotonic solution containing 150 mM KCl, 10 mM HEPES, and 200 µM CaCl2 at pH = 7.2. Mitoplasts are easily recognizable due to their size, round shape, transparency, and presence of a “cap”, characteristics that distinguish these structures from the cellular debris that is also present in the preparation. An isotonic solution containing 200 µM CaCl2 was used as the control solution for all of the presented data. The low-calcium solution (1 µM CaCl2) contained the following: 150 mM KCl, 10 mM HEPES, 1 mM EGTA and 0.752 mM CaCl2 at pH = 7.2. All of the modulators of the channels and the substrates and inhibitors of the respiratory chain were added as dilutions in isotonic solution containing 200 µM CaCl2. To apply these substances, we used a perfusion system containing a holder with a glass tube (made in our workshop), a peristaltic pump, and Teflon tubing. The mitoplasts at the tip of the measuring pipette were transferred into the openings of a multibarrel “sewer pipe” system in which their outer faces were rinsed with the test solutions (Fig. 1A ). The configuration of our patch-clamp mode is presented in Fig. 1A . The experiments were carried out in patch-clamp inside-out mode. This is based on observations with various mitochondrial substrates applied such as NADH or succinate. Reported voltages are those applied to the patch-clamp pipette interior. Hence, positive potentials represent the physiological polarization of the inner mitochondrial membrane (outside positive).
The electrical connection was made using Ag/AgCl electrodes and an agar salt bridge (3 M KCl) as the ground electrode. The current was recorded using a patch-clamp amplifier (Axopatch 200B, Molecular Devices Corporation, USA). The pipettes, made of borosilicate glass, had a resistance of 10–20 MΩ and were pulled using a Flaming/Brown puller.
The currents were low-pass filtered at 1 kHz and sampled at a frequency of 100 kHz. The traces of the experiments were recorded in single-channel mode. The illustrated channel recordings are representative of the most frequently observed conductance for the given condition. The conductance of the channel was calculated from the current-voltage relationship (data not shown). The probability of channel opening (Po, open probability) was determined using the single-channel search mode of the Clampfit 10.2 software. Calculations were performed using segments of continuous recordings lasting 60 s, with N>1000 events. Data from the experiments are reported as the mean values ± standard deviations (S.D.). Student’s t-test was used for statistical analysis. In figures showing single-channel recordings, “-” indicates the closed state of the channel.
The electrical connection was made using Ag/AgCl electrodes and an agar salt bridge (3 M KCl) as the ground electrode. The current was recorded using a patch-clamp amplifier (Axopatch 200B, Molecular Devices Corporation, USA). The pipettes, made of borosilicate glass, had a resistance of 10–20 MΩ and were pulled using a Flaming/Brown puller.
The currents were low-pass filtered at 1 kHz and sampled at a frequency of 100 kHz. The traces of the experiments were recorded in single-channel mode. The illustrated channel recordings are representative of the most frequently observed conductance for the given condition. The conductance of the channel was calculated from the current-voltage relationship (data not shown). The probability of channel opening (Po, open probability) was determined using the single-channel search mode of the Clampfit 10.2 software. Calculations were performed using segments of continuous recordings lasting 60 s, with N>1000 events. Data from the experiments are reported as the mean values ± standard deviations (S.D.). Student’s t-test was used for statistical analysis. In figures showing single-channel recordings, “-” indicates the closed state of the channel.
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Agar
Astrocytoma
Calcium
Cellular Structures
Egtazic Acid
Electricity
Face
HEPES
Homo sapiens
Hypertonic Solutions
Hypotonic Solutions
inhibitors
Isotonic Solutions
Medical Devices
Mitochondria
Mitochondrial Membrane, Inner
NADH
Perfusion
Peristalsis
physiology
Respiratory Chain
Sodium Chloride
Student
Succinate
Technique, Dilution
Teflon
Tissue, Membrane
Cells of the immortalised mesenchymal stromal cell line N-KM (normal bone marrow) [41 ] were cultured in 6- or 24-well plates in 10% FBS, 100 U/mL penicillin, 100 U/mL streptomycin and 100 U/mL glutamine (Life Technologies) supplemented IMDM (Lonza, Cologne, Germany) until they reached approximately 70% confluency.
For the analyses of EV uptake equivalents of 1 × 108 particles of the EV-enriched samples were added to the cells. After incubation for 14–16 h at 37°C, the medium with residual particles was removed and fresh culture media were added. At first, cells were analysed by fluorescent microscopy on an Axio Observer.D1 microscope platform with Plan-Apochromat 20×/0.8 lenses (Zeiss, Oberkochen, Germany). To harvest cells for flow cytometric analysis, cells were treated with 0.25% trypsin (Lonza) for 5 min at 37°C. The enzymatic reaction was stopped by the addition of fresh culture media. Cells were pelleted by centrifugation for 5 min at 800 × g, re-suspended in isotonic solution for flow cytometry (Beckman Coulter) and analysed on a Cytomics FC500 flow cytometer (Beckman Coulter) for their eGFP-intensity. The mean fluorescence intensity was measured for all samples in comparison to untreated N-KM cells.
For the analyses of EV uptake equivalents of 1 × 108 particles of the EV-enriched samples were added to the cells. After incubation for 14–16 h at 37°C, the medium with residual particles was removed and fresh culture media were added. At first, cells were analysed by fluorescent microscopy on an Axio Observer.D1 microscope platform with Plan-Apochromat 20×/0.8 lenses (Zeiss, Oberkochen, Germany). To harvest cells for flow cytometric analysis, cells were treated with 0.25% trypsin (Lonza) for 5 min at 37°C. The enzymatic reaction was stopped by the addition of fresh culture media. Cells were pelleted by centrifugation for 5 min at 800 × g, re-suspended in isotonic solution for flow cytometry (Beckman Coulter) and analysed on a Cytomics FC500 flow cytometer (Beckman Coulter) for their eGFP-intensity. The mean fluorescence intensity was measured for all samples in comparison to untreated N-KM cells.
Bone Marrow
Cells
Centrifugation
Culture Media
Enzymes
Flow Cytometry
Fluorescence
G-800
Glutamine
Isotonic Solutions
Lens, Crystalline
Mesenchymal Stromal Cells
Microscopy
Penicillins
Streptomycin
Trypsin
General anesthesia of tumor-bearing mice was induced with inhalation of isoflurane in 40 % oxygen/60 % nitrogen (gas flow = 1 mL min−1), and the mice were subsequently fixed in prone position. The body temperature was kept constant at 37 °C for the entire experiment. The mice were positioned in a prone position into the center of the field of view. A transmission scan for attenuation correction was not acquired. The mice were injected with 2–10 MBq of [18F]DCFPyL (60–150 ng) in 100–200 μL of isotonic NaCl solution (0.9 %) through a tail vein catheter. For blocking studies, the animals were pre-dosed with 300 μg of DCFPyL in 50 μL saline about 10 min prior to radiotracer injection. Data acquisition was performed over 60 min in a 3D list mode. The dynamic list mode data were sorted into sinograms with 53 time frames (10 × 2, 8 × 5, 6 × 10, 6 × 20, 8 × 60, 10 × 120, 5 × 300 s). The frames were reconstructed using maximum a posteriori (MAP) as reconstruction mode. The pixel size was 0.085 × 0.085 × 0.121 mm3 (256 × 256 × 63), and the resolution in the center of the field of view was 1.8 mm. No correction for partial volume effects was applied. The image files were processed using the ROVER v 2.0.51 software (ABX GmbH, Radeberg, Germany). Masks defining 3D regions of interest (ROI) were set and the ROIs were defined by thresholding.
Mean standardized uptake values [SUV]mean = (activity/mL tissue)/(injected activity/body weight), mL/g, were calculated for each ROI. Time-activity curves (TACs) were generated for the dynamic scans only. All semi-quantified PET data are presented as means ± SEM. Statistical difference for the blocking study was tested by unpaired Student’s t test and was considered significant for P < 0.05.
Mean standardized uptake values [SUV]mean = (activity/mL tissue)/(injected activity/body weight), mL/g, were calculated for each ROI. Time-activity curves (TACs) were generated for the dynamic scans only. All semi-quantified PET data are presented as means ± SEM. Statistical difference for the blocking study was tested by unpaired Student’s t test and was considered significant for P < 0.05.
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Animals
Body Temperature
Body Weight
Cardiac Arrest
Catheters
General Anesthesia
Inhalation
Isoflurane
Isotonic Solutions
Mus
Neoplasms
Nitrogen
Oxygen
Radionuclide Imaging
Reading Frames
Reconstructive Surgical Procedures
Saline Solution
Sodium Chloride
Student
Tail
Tissues
Transmission, Communicable Disease
Veins
Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient (GE Health Care, Uppsala, Sweden) centrifugation. PBMCs (1.0 × 106 cells/mL) were incubated with 10.0 μg/mL TLR3 agonist (Poly I:C hmw; InvivoGen, San Diego-CA, USA) or 5.0 μg/mL TLR7/8 agonist (CL097; InvivoGen), and CD107a PE-Cy5 (Pe.Cy5/Clone: H4A3) was added to detect degranulating NK cells. The samples were plated and incubated at 37 °C with 5% CO2 for 6 h. After 2 h of incubation, Brefeldin (10.0 µg/mL; Sigma, St. Louis-MO, USA) was added to the cultures for another 4 h of incubation. After incubation, the cells were washed and incubated with human IgG for 15 min, followed by incubation with Live-Dead reagent (Invitrogen, Eugene, OR, USA) for 20 min at room temperature. Cells were then subjected to fixation with Cytofix/Cytoperm solution (BD Bioscience, San Diego, CA, USA) for 20 min and permeabilization with Perm/Wash solution for 20 min at 4 °C. The cells were then stained with CD3 (BV605/Clone: SK7), CD19 (Horizon V500/Clone: HIB19), CD16 (APC-Cy7/Clone: 3G8), CD56 (Alexa 700/Clone: B159), CD62L (FITC/Clone: Dreg 56), CD38 (PE/Clone: HIT2), IFN-γ (Horizon V450/Clone: B27) and TNF (PE-Cy7/Clone: MAB11) antibodies. Next, the samples were washed with Perm/Wash buffer (BD Bioscience, San Diego, CA, USA) and diluted in isotonic solution. Fluorescence Minus One (FMO) controls were performed for all antibody panels to confirm proper compensation and define positive signals. Boolean gate arrays were created using FlowJo software. A total of 300,000 events were acquired and analysed by flow cytometry (LSR Fortessa, BD Biosciences, USA). These analyses determined the expression frequency of each cytokine based on all possible combinations of the 3 different cytokines. Analysis of polychromatic flow cytometry data was performed with the SPICE Program (version 2.9, Vaccine Research Center, NIAID, USA).
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A 300
Antibodies
Buffers
Cells
Centrifugation
Clone Cells
Cytokine
Ficoll
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
Homo sapiens
Hypaque
Immunoglobulins
Interferon Type II
Isotonic Solutions
Natural Killer Cells
PBMC Peripheral Blood Mononuclear Cells
Poly I-C
Progressive Encephalomyelitis with Rigidity
SELL protein, human
Spices
Vaccines
Most recents protocols related to «Isotonic Solutions»
All injections were performed in the operating room. The standard injection technique applied to all patients is as follows: Eyelids and around the eyes were wiped with a 10% sterile gauze pad impregnated with povidone-iodine. Proparacaine hydrochloride (Alcaine) was dropped for topi-cal anesthesia. After placing the sterile eyelid retractor, 5% povidone-iodine was added to the eye surface and left for 3 min and then washed with a sterile isotonic solution. Four mm from the limbus in phakic eyes and 3.5 mm from the limbus in pseudophakic eyes were marked with compasses. Superotemporal quadrant was tried to be preferred as the entry point. 0.1 mL (0.5 mg) RAN was injected from the point determined by the compass with the 30 gauge needle toward the center of the vitreous cavity. The same procedure was followed in the DEX group, but additionally, subconjunctival anesthesia was applied and the DEX implant was injected into the vitreous with a 22 gauge applicator.
A short-term gentle pressure was applied to the injection site with a cotton-tipped applicator immediately after the injection to prevent the drug or vitreous from leaking back and bleeding from the conjunctiva. The tone of the eye was controlled digitally. Whether there was a sense of light was questioned. Antibiotic drops were given to all patients for one week and they were warned to apply to the emergency department if they have complaints such as sudden vision decrease, pain, and redness. Patients were called for control the next day and examined for infection and sudden IOP increase.
A short-term gentle pressure was applied to the injection site with a cotton-tipped applicator immediately after the injection to prevent the drug or vitreous from leaking back and bleeding from the conjunctiva. The tone of the eye was controlled digitally. Whether there was a sense of light was questioned. Antibiotic drops were given to all patients for one week and they were warned to apply to the emergency department if they have complaints such as sudden vision decrease, pain, and redness. Patients were called for control the next day and examined for infection and sudden IOP increase.
Alcaine
Anesthesia
Antibiotics
Conjunctiva
Dental Caries
Erythema
Eyelids
Gossypium
Infection
Isotonic Solutions
Light
Low Vision
Needles
Pain
Patients
Pharmaceutical Preparations
Povidone Iodine
Pressure
proparacaine hydrochloride
Sterility, Reproductive
TOP1 protein, human
Protocol full text hidden due to copyright restrictions
Open the protocol to access the free full text link
1,2-dioleoyloxy-3-(trimethylammonium)propane
Cholesterol
Freezing
Isotonic Solutions
Lipid Droplet
Molar
N-palmitoylsphingosine
Nitrogen
Nucleic Acids
Phosphates
RNA, Small Interfering
Solvents
Sterility, Reproductive
Sucrose
tert-Butyl Alcohol
While a positive DFAT result indicates an infection, a negative result does not rule out the possibility of infection. Therefore, after microscopic observation of the negative samples, the brain tissues were prepared and the mouse inoculation test (MIT) was performed. The MIT was conducted by adding a clarified supernatant of a homogenate of the brain material in an isotonic buffered solution containing antibiotics (10 ml of brain suspension with 50 μl of penicillin and 20 μl of streptomycin). Then, the skull of ten mice, 3–4 weeks old (12–14 grams), was injected with the suspension of 50 μl of the brain sample. The mice were anesthetized by ether before inoculation. For intracerebral inoculation, sterilized needles (27 and 26 gauges) were used. Next, the mice were observed daily for 28 days. Any deaths occurring during the first 4 days were regarded as nonspecific, and every dead mouse after the 4th day was considered positive, and the brain was obtained and sent for the DFAT examination [17 (link), 18 (link)].
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Antibiotics
Brain
Cranium
Ethyl Ether
Infection
Isotonic Solutions
Mice, House
Microscopy
Needles
Penicillins
Streptomycin
Tissues
Vaccination
Semen samples were allowed to liquefy for 20 min at room temperature. Seminal plasma, containing exosomes, was separated from the cell fraction by centrifugation at 1000 ×g for 10 min at 20°C. Cell debris was removed by subsequent centrifugation at 2400×g for 30 min at 20°C followed by 0.45 and 0.22 μm syringe filtration (Millex HA, Darmstadt, Germany). The cell pellet was cryopreserved (both in PWID and control semen samples) in 1 ml of freezing medium containing 90% heat-inactivated fetal bovine serum (Nucleus Biologics, San Diego, CA, USA) and 10% dimethyl sulfoxide. Cryovials were placed in a –80°C freezer in a Mr. Frosty™ freezing device containing isopropanol; the approximate freezing rate was –1°C/min. For long-term storage, cryovials were transferred to vapor-phase nitrogen.
Mature spermatozoa were purified using an adapted Percoll gradient protocol (Claassens et al., 1998 (link)). Isotonic Percoll solution was prepared by mixing 9 ml of Percoll (Sigma-Aldrich, St. Louis, MO, USA) with 1 ml of 10× concentrated Ham’s F10 solution (MP Biomedicals, Solon, OH, USA). The bottom fraction of the Percoll gradient (90%) contained 1 ml of 90% isotonic Percoll mixed with 10% X-VIVO 15 (Lonza, Basel, Switzerland) or human tubal fluid ‘HTF’ culture media (in-house). The upper fraction (45%) contained 1 ml of 45% isotonic Percoll mixed with 55% X-VIVO 15 (Lonza, Basel, Switzerland) or HTF culture media (in-house). The gradient was prepared in 15 ml Falcon conical tubes. Semen samples from both groups (PWID and controls) were thawed quickly in a 37°C water bath and 1 ml of X-VIVO 15 was added dropwise to each cryovial. Thawed semen samples were washed in 25 ml of X-VIVO 15, centrifuged at 400×g for 10 min at 20°C, resuspended in ∼500 µl of X-VIVO15 and layered on top of the gradient and centrifuged again at 400×g for 20 min (brake off). After centrifugation, ∼1.5 ml of the supernatant was aspirated off and discarded. The pellet and remaining ∼1 ml of Percoll were washed with 14 ml of X-VIVO 15 or PBS and spun down (400×g for 10 min at 20°C). The final pellet was resuspended in 500 µl of X-VIVO 15 and used to prepare smears on coverslips for Wright and hematoxylin and eosin (H&E) staining and/or aliquoted and frozen at –80°C for later RNA purification. Both stainings were performed at the Histology and Imaging Core (HIC) of the University of Washington, South Lake Union campus (Seattle, WA, USA).
Mature spermatozoa were purified using an adapted Percoll gradient protocol (Claassens et al., 1998 (link)). Isotonic Percoll solution was prepared by mixing 9 ml of Percoll (Sigma-Aldrich, St. Louis, MO, USA) with 1 ml of 10× concentrated Ham’s F10 solution (MP Biomedicals, Solon, OH, USA). The bottom fraction of the Percoll gradient (90%) contained 1 ml of 90% isotonic Percoll mixed with 10% X-VIVO 15 (Lonza, Basel, Switzerland) or human tubal fluid ‘HTF’ culture media (in-house). The upper fraction (45%) contained 1 ml of 45% isotonic Percoll mixed with 55% X-VIVO 15 (Lonza, Basel, Switzerland) or HTF culture media (in-house). The gradient was prepared in 15 ml Falcon conical tubes. Semen samples from both groups (PWID and controls) were thawed quickly in a 37°C water bath and 1 ml of X-VIVO 15 was added dropwise to each cryovial. Thawed semen samples were washed in 25 ml of X-VIVO 15, centrifuged at 400×g for 10 min at 20°C, resuspended in ∼500 µl of X-VIVO15 and layered on top of the gradient and centrifuged again at 400×g for 20 min (brake off). After centrifugation, ∼1.5 ml of the supernatant was aspirated off and discarded. The pellet and remaining ∼1 ml of Percoll were washed with 14 ml of X-VIVO 15 or PBS and spun down (400×g for 10 min at 20°C). The final pellet was resuspended in 500 µl of X-VIVO 15 and used to prepare smears on coverslips for Wright and hematoxylin and eosin (H&E) staining and/or aliquoted and frozen at –80°C for later RNA purification. Both stainings were performed at the Histology and Imaging Core (HIC) of the University of Washington, South Lake Union campus (Seattle, WA, USA).
Bath
Biological Factors
Cell Nucleus
Cells
Centrifugation
Culture Media
Eosin
Exosomes
Fetal Bovine Serum
Filtration
Freezing
Hematoxylin
Homo sapiens
Isopropyl Alcohol
Isotonic Solutions
Medical Devices
Nitrogen
Percoll
Plant Embryos
Seminal Plasma
Solon
Sperm
Staining
Sulfoxide, Dimethyl
Syringes
Heart beating (brain death), non-heart-beating and exitus donors are screened for heart valves and vascular segments donation. A maximum warm ischemia time of 24 h is accepted if the body has been refrigerated within 6 h after asystolia, or 12 h if the body has not been refrigerated. Hearts from living donors (who have received a heart transplant) can also be obtained.
Complete donor evaluation is performed in order to discard any general or specific contraindication for CV donation. This evaluation includes, but is not limited to, a review of the medical and social history, serological and microbiological testing for transmissible diseases, physical examination of the body, autopsy findings if apply, as well as any other relevant information provided by relatives or team leader responsible for the retrieval. Compulsory serology testing includes HIV, hepatitis B and C, syphilis, HTLV and nucleic acid determination of HIV, hepatitis B and C, and nowadays also hepatitis E. Additionally, depending on the country of origin of the donor, the information obtained from the relatives, the travel history and the epidemiologic situation, this serology could be completed with additional determinations such as Trypanosoma Cruzy or West Nile Virus.
After complete donor evaluation and next of kin donation consent (or donor himself if living donation), tissue donation is performed. Tissue retrieval is always performed in an operating room (OR). BTB has the possibility of performing the retrieval in diferent locations: (a) specific facilities for tissue recovery located in two diferent hospitals and IMLCFC; (b) OR in hospitals authorized for tissue donation; or (C) transplant hospitals in case of living donation.
CV tissue is procured by the multi-tissue retrieval teams but, in heart-beating organ donors, the tissue can also be procured by the organ transplant team.
In multi-tissue donors, CV tissues are recovered simultaneously to muskulosqueletal, opening the thoracic cavity by esternotomy and the abdominal cavity, after ocular and skin tissue retrieval. Heart is retrieved preserving the aortic arch and pulmonary branches as long as possible. Standard recovered vascular segments are aorto-iliac bifurcation and femoral arteries, but other segments from descending thoracic aorta to abdominal aorta can be also recovered depending on the needs.
After recovery, CV tissues (heart and vascular segments) are packaged separately into sterile containers with isotonic solution (ringer lactate, saline solution), each one wrapped in sterile bag and kept in a thermobox to ensure a temperature between + 2/+8ºC. The container is labelled with Single European Code (given by the IT system in the DC) and forwarded to TE, where it is processed not later than 32 h after retrieval. The tissue is accompanied by documentation that guarantee full traceability, including the retrieval form with some compulsory identification data: donor ID, donation time and date, asystolia and retrieval data.
Complete donor evaluation is performed in order to discard any general or specific contraindication for CV donation. This evaluation includes, but is not limited to, a review of the medical and social history, serological and microbiological testing for transmissible diseases, physical examination of the body, autopsy findings if apply, as well as any other relevant information provided by relatives or team leader responsible for the retrieval. Compulsory serology testing includes HIV, hepatitis B and C, syphilis, HTLV and nucleic acid determination of HIV, hepatitis B and C, and nowadays also hepatitis E. Additionally, depending on the country of origin of the donor, the information obtained from the relatives, the travel history and the epidemiologic situation, this serology could be completed with additional determinations such as Trypanosoma Cruzy or West Nile Virus.
After complete donor evaluation and next of kin donation consent (or donor himself if living donation), tissue donation is performed. Tissue retrieval is always performed in an operating room (OR). BTB has the possibility of performing the retrieval in diferent locations: (a) specific facilities for tissue recovery located in two diferent hospitals and IMLCFC; (b) OR in hospitals authorized for tissue donation; or (C) transplant hospitals in case of living donation.
CV tissue is procured by the multi-tissue retrieval teams but, in heart-beating organ donors, the tissue can also be procured by the organ transplant team.
In multi-tissue donors, CV tissues are recovered simultaneously to muskulosqueletal, opening the thoracic cavity by esternotomy and the abdominal cavity, after ocular and skin tissue retrieval. Heart is retrieved preserving the aortic arch and pulmonary branches as long as possible. Standard recovered vascular segments are aorto-iliac bifurcation and femoral arteries, but other segments from descending thoracic aorta to abdominal aorta can be also recovered depending on the needs.
After recovery, CV tissues (heart and vascular segments) are packaged separately into sterile containers with isotonic solution (ringer lactate, saline solution), each one wrapped in sterile bag and kept in a thermobox to ensure a temperature between + 2/+8ºC. The container is labelled with Single European Code (given by the IT system in the DC) and forwarded to TE, where it is processed not later than 32 h after retrieval. The tissue is accompanied by documentation that guarantee full traceability, including the retrieval form with some compulsory identification data: donor ID, donation time and date, asystolia and retrieval data.
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Abdominal Cavity
Aortas, Abdominal
Arch of the Aorta
Autopsy
Blood Donation
Blood Vessel
Brain Death
Cardiac Arrest
Compulsive Behavior
Donor, Organ
Donors
Europeans
Femoral Artery
Grafts
Heart
Heart Transplantation
Heart Valves
Hepatitis B
Hepatitis E
Human Body
Ilium
Isotonic Solutions
Lactated Ringer's Solution
Living Donors
Lung
Nucleic Acids
Organ Transplantation
Physical Examination
Saline Solution
Skin
Sterility, Reproductive
Syphilis
T-Cell Leukemia Viruses, Human
Thoracic Aorta
Thoracic Cavity
Tissue Donors
Tissues
Trypanosoma
Vision
West Nile virus
Top products related to «Isotonic Solutions»
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Isotonic Percoll solution is a laboratory reagent used for the separation and isolation of cells, organelles, and other biological particles. It is a colloidal silica suspension with a specific gravity that can be adjusted to facilitate the separation of different cellular components or cell types based on their density.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Fura-2 AM is a fluorescent calcium indicator used for measuring intracellular calcium levels. It is a cell-permeable derivative of the parent compound Fura-2. Fura-2 AM can be loaded into cells, where intracellular esterases cleave off the acetoxymethyl (AM) ester group, trapping the Fura-2 indicator inside the cell.
Sourced in Germany
The Osmomat 030 is a laboratory instrument designed for the precise measurement of osmotic pressure. It operates using the freezing point depression principle to determine the osmotic concentration of a liquid sample.
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DNase I is a laboratory enzyme that functions to degrade DNA molecules. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively breaking down DNA strands.
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FBS, or Fetal Bovine Serum, is a commonly used cell culture supplement. It is derived from the blood of bovine fetuses and provides essential growth factors, hormones, and other nutrients to support the growth and proliferation of a wide range of cell types in vitro.
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Percoll is a colloidal silica-based medium used for cell separation and gradient centrifugation. It is designed to provide a density gradient for the isolation and purification of cells, organelles, and other biological particles.
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The Zetasizer Nano ZS is a dynamic light scattering (DLS) instrument designed to measure the size and zeta potential of particles and molecules in a sample. The instrument uses laser light to measure the Brownian motion of the particles, which is then used to calculate their size and zeta potential.
Sourced in United States
LysoSensor Yellow/Blue DND-160 is a fluorescent probe used for staining and imaging acidic organelles, such as lysosomes, in live cells. It exhibits a pH-dependent shift in fluorescence emission, allowing for the visualization and analysis of lysosomal pH.
Sourced in United States
[1-13C]pyruvic acid is a stable isotope-labeled chemical compound. It is used as a precursor for the synthesis of other compounds in various research and analytical applications.
More about "Isotonic Solutions"
Isotonic solutions, also known as physiological solutions or balanced salt solutions, are aqueous mixtures that have the same osmotic pressure as body fluids.
These solutions are widely used in medical and research settings to maintain cell viability and prevent osmotic stress.
Isotonic Percoll solution, which contains Percoll in an isotonic buffer, is commonly used for cell separation and purification.
DMSO (Dimethyl sulfoxide) is another important isotonic agent used to cryopreserve cells and tissues.
Fura-2 AM is a fluorescent calcium indicator that is often used in isotonic solutions to measure intracellular calcium levels.
The Osmomat 030 is a laboratory instrument used to measure the osmolarity of isotonic solutions, ensuring they have the correct osmotic pressure.
DNase I, an enzyme that digests DNA, is sometimes added to isotonic solutions to prevent cell clumping.
FBS (Fetal Bovine Serum) is a common additive in isotonic cell culture media, providing essential nutrients and growth factors.
Percoll is a colloidal silica-based medium used to create density gradients for cell separation in isotonic solutions.
The Zetasizer Nano ZS is a tool used to measure the size and zeta potential of particles in isotonic solutions, which is important for understanding their behavior.
LysoSensor Yellow/Blue DND-160 is a fluorescent dye that can be used in isotonic solutions to detect and measure the pH of lysosomes within cells. [1-13C]pyruvic acid is a labeled metabolite that can be used in isotonic solutions to study cellular metabolism.
Experince the future of research today with PubCompare.ai's powerful tools and data-driven insights to optimize your isotonic solution protocols and enhance the reproducibility of your studies.
These solutions are widely used in medical and research settings to maintain cell viability and prevent osmotic stress.
Isotonic Percoll solution, which contains Percoll in an isotonic buffer, is commonly used for cell separation and purification.
DMSO (Dimethyl sulfoxide) is another important isotonic agent used to cryopreserve cells and tissues.
Fura-2 AM is a fluorescent calcium indicator that is often used in isotonic solutions to measure intracellular calcium levels.
The Osmomat 030 is a laboratory instrument used to measure the osmolarity of isotonic solutions, ensuring they have the correct osmotic pressure.
DNase I, an enzyme that digests DNA, is sometimes added to isotonic solutions to prevent cell clumping.
FBS (Fetal Bovine Serum) is a common additive in isotonic cell culture media, providing essential nutrients and growth factors.
Percoll is a colloidal silica-based medium used to create density gradients for cell separation in isotonic solutions.
The Zetasizer Nano ZS is a tool used to measure the size and zeta potential of particles in isotonic solutions, which is important for understanding their behavior.
LysoSensor Yellow/Blue DND-160 is a fluorescent dye that can be used in isotonic solutions to detect and measure the pH of lysosomes within cells. [1-13C]pyruvic acid is a labeled metabolite that can be used in isotonic solutions to study cellular metabolism.
Experince the future of research today with PubCompare.ai's powerful tools and data-driven insights to optimize your isotonic solution protocols and enhance the reproducibility of your studies.