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Versene

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Most cited protocols related to «Versene»

1
Differentiation of hPSC-Derived Brain Microvascular Endothelial Cells
Cited 40 times
IMR90-4 and DF19-9-11T iPSCs and H9 hESCs were maintained between passages 26–42 on Matrigel (BD Biosciences) in mTeSR1 medium (STEMCELL Technologies) or on irradiated mouse embryonic fibroblasts (MEFs) in standard unconditioned medium (UM) as previously described18 (link). For differentiation, cells were passaged onto Matrigel in mTeSR1 medium for 2–3 days of expansion and then switched to unconditioned medium (UM) lacking bFGF for 6 days. Human endothelial serum-free medium (hESFM; Life Technologies) supplemented with 20 ng/mL bFGF (R&D Systems) and 1% platelet-poor plasma derived bovine serum (Biomedical Technologies, Inc.) was then added for an additional 2–4 days. All-trans RA (Sigma) was reconstituted in DMSO and included at concentrations of 1–10 μM depending on the experiment. Cells were then dissociated with Versene (Life Technologies) and plated onto 12-well tissue culture polystyrene plates or 1.12 cm2 Transwell-Clear® permeable inserts (0.4 μm pore size) coated with a mixture of collagen IV (400 μg/mL; Sigma) and fibronectin (100 μg/mL; Sigma) in H2O. Culture plates were incubated with the coating for at least 30 min at 37°C, while the inserts were incubated for a minimum of 4 h at 37°C. Resultant, purified hPSC-derived BMECs were then grown in EC medium for 24 h (with or without RA); in some experiments, primary pericytes or fibroblasts were co-cultured with BMECs during these 24 h (see description below). After this 24 h period, BMECs were continued as monoculture or co-cultured as described below. In our previous publication, we had utilized dispase for subculturing the BMECs18 (link), but non-enzymatic treatment of the BMECs with Versene results in less debris attached to the BMEC monolayer. We had also used hPSCs exclusively maintained on MEFs prior to differentiation18 (link), but in this study no noticeable differences in BBB properties were observed between hPSCs maintained on MEFs and hPSCs maintained under feeder-independent conditions and we now exclusively use hPSCs maintained in mTeSR1 on Matrigel.
Lippmann E.S., Al-Ahmad A., Azarin S.M., Palecek S.P, & Shusta E.V. (2014). A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources. Scientific Reports, 4, 4160.
Publication 2014
Biomedical Technology Blood Platelets Bos taurus Cells Collagen Type IV dispase Embryo Endothelium Enzymes Fibroblasts FN1 protein, human Homo sapiens Human Embryonic Stem Cells Induced Pluripotent Stem Cells matrigel Mus Pericytes Permeability Plasma Polystyrenes Serum Stem Cells Sulfoxide, Dimethyl Tissues Versene
2
GPCR Activation Monitoring via BRET2
Cited 34 times
Cells were plated either in six-well dishes at a density of 700,000–800,000 cells/well, or 10-cm dishes at 7–8 million cells/dish. Cells were transfected 2–4 hours later, using a 1:1:1:1 DNA ratio of receptor:Gα-RLuc8:Gβ:Gγ-GFP2 (100 ng/construct for six-well dishes, 750 ng/construct for 10-cm dishes), except for the Gγ-GFP2 screen, where an ethanol co-precipitated mixture of Gβ1–4-was used at twice its normal ratio (1:1:2:1). Transit 2020 (Mirus Biosciences, Madison, WI) was used to complex the DNA at a ratio of 3 μL Transit/μg DNA, in OptiMEM (Gibco-ThermoFisher, Waltham, MA) at a concentration of 10 ng DNA/μL OptiMEM. The next day, cells were harvested from the plate using Versene (0.1M PBS + 0.5 mM EDTA, pH 7.4), and plated in poly-D-lysine-coated white, clear bottom 96-well assay plates (Greiner Bio-One, Monroe, NC) at a density of 30,000–50,000 cells/well.
One day after plating in 96-well assay plates, white backings (Perkin Elmer, Waltham, MA) were applied to the plate bottoms, and growth medium was carefully aspirated and replaced immediately with 60 μL of assay buffer (1x HBSS + 20 mM HEPES, pH 7.4), followed by a 10 μL addition of freshly prepared 50 μM coelenterazine 400a (Nanolight Technologies, Pinetop, AZ). After a five-minute equilibration period, cells were treated with 30 μL of drug for an additional 5 minutes. Plates were then read in an LB940 Mithras plate reader (Berthold Technologies, Oak Ridge, TN) with 395 nm (RLuc8-coelenterazine 400a) and 510 nm (GFP2) emission filters, at 1 second/well integration times. Plates were read serially six times, and measurements from the sixth read were used in all analyses. BRET2 ratios were computed as the ratio of the GFP2 emission to RLuc8 emission.
Olsen R.H., DiBerto J.F., English J.G., Glaudin A.M., Krumm B.E., Slocum S.T., Che T., Gavin A.C., McCorvy J.D., Roth B.L, & Strachan R.T. (2020). “TRUPATH, an Open-Source Biosensor Platform for Interrogating the GPCR Transducerome”. Nature chemical biology, 16(8), 841-849.
Publication 2020
Biological Assay Buffers Cells coelenterazine Culture Media DNA receptor Edetic Acid Ethanol Hemoglobin, Sickle HEPES Hyperostosis, Diffuse Idiopathic Skeletal Lysine Pharmaceutical Preparations Poly A Versene
3
Directed Differentiation of Human iPSCs and ESCs
Cited 21 times
Human iPSCs and ESCs maintained on MEFs were passaged on Matrigel (GFR, BD Biosciences) thin coated 6-well plates (8.7 ug/cm2) and cultured in mTeSR1 medium (WiCell Institute) for 5–6 days to deplete the feeder cells. Cells were washed with PBS and incubated with 1 ml/well Versene solution (Invitrogen) at 37°C for 5 minutes to singularize the cells and seeded on Matrigel coated plate at the density of 100,000 cells/cm2 in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, CalBiochem). The medium was changed daily, and after 3–4 days when the monolayer of cells reached 80–90% confluence, a thin layer of Matrigel was overlaid by freshly mixing Matrigel (GFR), 0.5 mg (faster growing lines, i.e. DF19-9-11T, DF19-9-7T, IMR90 C4 or H9) or 1 mg (slower growing lines, i.e. DF6-9-9T, H1), in 15 ml ice cold mTeSR1 medium and replacing the medium in each well of a 6-well plate with 2.5 ml of Matrigel containing mTeSR1. Cells were cultured in mTeSR1 medium for another 1–2 days until the cells were 100% confluent, which is referred to as day 0 when the medium was replaced with 2.5 ml of RPMI 1640 basal medium (Invitrogen) plus B-27 without insulin supplement (Invitrogen) containing Activin A (100 ng/ml, R&D Systems) and Matrigel (0.5 mg Matrigel/6-well plate). After 24 hours, the medium was changed with the same medium as day 0 (3 ml/well) without Matrigel but supplemented with BMP4 (5–10 ng/ml, R&D Systems) and bFGF (5–10 ng/ml, Invitrogen) for another 4 days without medium change. At day 5, the medium was changed to RPMI plus B27 complete supplement (Invitrogen), and the medium was changed every 2–3 days. For H9 cells, 1% KnockOut serum replacer (Invitrogen) was added at day 0 in a subset of experiments.
Zhang J., Klos M., Wilson G.F., Herman A.M., Lian X., Raval K.K., Barron M.R., Hou L., Soerens A.G., Yu J., Palecek S.P., Lyons G.E., Thomson J.A., Herron T.J., Jalife J, & Kamp T.J. (2012). Extracellular Matrix Promotes Highly Efficient Cardiac Differentiation of Human Pluripotent Stem Cells: The Matrix Sandwich Method. Circulation research, 111(9), 1125-1136.
Publication 2012
activin A Bone Morphogenetic Protein 4 Cells Common Cold Dietary Supplements Enhanced S-Cone Syndrome Feeder Cells Homo sapiens Induced Pluripotent Stem Cells Insulin matrigel Serum Versene Y 27632
4
Preparation of Rat Neuronal Cultures
Cited 9 times
Rat neurons were prepared as described previously (31 (link)) with some modifications. In brief, cerebella of 7-day-postnatal Sprague-Drawly rats were dissociated in Versene solution (1:5000, Invitrogen) and total cells (1 × 106 cells per 35 mm2) were plated in poly-D-Lysine (Sigma, St. Louis, MO) coated plates or dishes for five minutes after which, the non-adherent cell suspension was removed and used for preparing glial cells. Adherent cells were maintained in MEM (Mediatech, Herndon, VA) supplemented with heat-inactivated 10% fetal bovine serum (FBS, Atlas Biologicals, Fort Collins, CO), 25 mM KCl, 3gm/500 glucose (Sigma), and 1% antibiotic-antimycotic solution (Sigma). Cytosine-D-arabinoside (10 μM Ara-C, Sigma) was added to cultures 24 hrs after plating to block the proliferation of non-neuronal cells. These neurons were routinely used during 9–12 days in vitro (DIV). Ara-C was avoided to obtain neuron-glial mix culture (where glial growth was allowed in the neuronal culture). Rat hippocampal and cortical neurons were obtained from E18 embryos. The hippocampi or the cortices were dissected and dissociated in HBSS solution. Then they were plated in Poly-D-Lysine (Sigma) coated plates or dishes in Neurobasal media supplemented with B27 (Invitrogen). These neurons were routinely used during 14–21 days in vitro (DIV).
Saha R.N., Ghosh A., Palencia C.A., Fung Y.K., Dudek S.M, & Pahan K. (2009). Tumor necrosis factor-α preconditioning protects neurons via neuron-specific upregulation of CREB-binding protein. Journal of immunology (Baltimore, Md. : 1950), 183(3), 2068-2078.
Publication 2009
Antibiotics Ara-C Biological Factors Cardiac Arrest Cell Proliferation Cells Cerebellum Cortex, Cerebral Cytosine Embryo Glucose Hemoglobin, Sickle Hyperostosis, Diffuse Idiopathic Skeletal Lysine Neuroglia Neurons Poly A Seahorses Versene
5
Cardiomyocyte Differentiation from Urine-derived iPSCs
Cited 8 times

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Publication 2013
Cells Glutamine Hyperostosis, Diffuse Idiopathic Skeletal Induced Pluripotent Stem Cells Insulin matrigel Myocytes, Cardiac Urine Versene

Most recents protocols related to «Versene»

1
Cultivation of BWZ/hTREM-1 reporter cells
Not available on PMC !

Example 2

BWZ/hTREM-1 reporter cell were cultured in RPMI 1640 w/o phenol red (Cat #11835, Gibco, Carlsbad Calif., USA), supplemented with 10% FCS (Cat #16140-071, Gibco, New York, USA), 1% Pen/Strep (Cat #15070-06, Gibco), 1 mM Sodium Pyruvate (Cat #11360, Gibco), 5 μM-2ME (Cat #31350-010, Gibco) and 2 mM L-Glutamine (Cat #25030, Gibco). No special plates or coating was required. 10 ml Versene (Cat #15040, Gibco) was added to detach the cells which then were transferred to tubes, centrifuged 1200 rpm 5 min and washed in fresh RPMI 1640 w/o phenol red. These cell were then ready to use in an assay or re-culture for further propagation.

US11858992B2. Nucleic acids encoding antibodies that bind and block triggering receptor expressed on myeloid cells-1 (TREM-1) (2024-01-02). Novo Nordisk [DK]. Inventors: Vibeke Westphal Stennicke [DK], Christine Brender Read [DK], Susanne Nedergaard Grell [DK], Charlotte Wiberg [SE], Rune Salbo [DK], Anette Henriksen [DK], Soeren Padkjaer [DK].
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Patent 2024
Biological Assay Cell Lines Cells Glutamine Pyruvate Sodium Streptococcal Infections Versene
2
HEK293T Cell Culture and RNA Isolation
We cultured HEK293T cells in RPMI medium supplemented with 10% fetal calf serum to 80% confluence in a T75. The cells were washed with 2 ml versene and incubated with 2 ml of trypsin for 3 minutes at 37 °C. We neutralized the mixture with 8 ml fresh medium. We centrifuged for 5 minutes at 2000 rcf at 4 °C and removed the supernatants. We resuspended the cells in 1 ml of QIAzol and flash-froze the mixture in liquid nitrogen.
Everaert C., Verwilt J., Verniers K., Vandamme N., Marcos Rubio A., Vandesompele J, & Mestdagh P. (2023). Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation. Biological Procedures Online, 25, 7.
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Publication 2023
Fetal Bovine Serum Freezing Nitrogen PRSS2 protein, human Versene
3
Murine Bone Marrow Macrophage Differentiation
Murine bone-marrow progenitors were obtained by sampling tibias and femur bones from 8 to 12-week-old C57BL/6NJ wild type, Tirap+/- and Tirap-/- mice. BMDMs were obtained by seeding 107 bone marrow cells in 75 cm2 flasks in RPMI 1640 Glutamax medium (Gibco) supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS, Gibco; RPMI-FBS) and 10% L929 cell supernatant containing Macrophage Colony-Stimulating Factor (M-CSF). Fresh medium was added every 3–4 days. After 7 days incubation at 37°C in an atmosphere containing 5% CO2, the BMDMs monolayer was rinsed with D-PBS and cells were harvested with Versene (Gibco). BMDM were resuspended into culture medium to be used for subsequent assays.
Belhaouane I., Pochet A., Chatagnon J., Hoffmann E., Queval C.J., Deboosère N., Boidin-Wichlacz C., Majlessi L., Sencio V., Heumel S., Vandeputte A., Werkmeister E., Fievez L., Bureau F., Rouillé Y., Trottein F., Chamaillard M., Brodin P, & Machelart A. (2023). Tirap controls Mycobacterium tuberculosis phagosomal acidification. PLOS Pathogens, 19(3), e1011192.
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Publication 2023
Atmosphere Biological Assay Bone Marrow Bone Marrow Cells Cells Culture Media Femur Macrophage Colony-Stimulating Factor Mus Tibia Versene
4
Murine Bone Marrow-Derived Macrophage Isolation and Stimulation

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Publication 2023
Bacteria Bone Marrow Bones Buffers Cells Centrifugation Dexamethasone Edetic Acid Erythrocytes Ethanol Femur Ficoll Humerus Macrophage Males Mus Operative Surgical Procedures Penicillins potassium bicarbonate Serum Streptomycin Tibia Versene
5
Mammosphere Formation Assay Protocol
First, 150,000 cells in 2 ml CGM were plated in a six-well plate. After 12-24 h, cells were treated with EtOH or TAM in the presence or absence of 40 µM CCG-203971 for 6 or 36 h in RGM. Cells were trypsinized with Versene, resuspended in 1 ml 1× DPBS and passed three times through a 25-gauge needle to obtain single-cell suspensions. Then, 10,000 cells were plated in six-well plates previously coated with Poly(2-hydrocyethyl methacrylate) (PolyHEMA) and incubated in 2 ml mammosphere medium composed of 1:1 DMEM/F12, 20 ng/ml human EGF, 40 µg/ml insulin, 500 ng/ml hydrocortisone, 1% penincilin/streptomycin (Merck, 15070-063) and 1:1 Supplement B-27 Minus Vitamin A (Gibco, 12587-010), filtered with a 0.45 µm filter (VWR, 514-4127). Six days after incubation in a 5% CO2 humidified incubator at 37°C, brightfield pictures were acquired using a 5× objective on a brightfield microscope. Each condition was evaluated in triplicates.
Faria L., Canato S., Jesus T.T., Gonçalves M., Guerreiro P.S., Lopes C.S., Meireles I., Morais-de-Sá E., Paredes J, & Janody F. (2023). Activation of an actin signaling pathway in pre-malignant mammary epithelial cells by P-cadherin is essential for transformation. Disease Models & Mechanisms, 16(2), dmm049652.
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Publication 2023
CCG-203971 Cells Dietary Supplements Ethanol Homo sapiens Hydrocortisone Insulin Methacrylate Microscopy Needles Poly A Streptomycin Versene Vitamin A

Top products related to «Versene»

1
Versene by Thermo Fisher Scientific
Sourced in United States, United Kingdom, France, Switzerland, Portugal, Germany
Versene is a chelating agent used in various laboratory applications. It binds to metal ions, effectively sequestering them from solutions. Versene is commonly used to remove unwanted metal ions, control pH, and stabilize solutions.
2
Versene solution by Thermo Fisher Scientific
Sourced in United States, France, United Kingdom
Versene solution is a chemical compound used in various laboratory applications. It functions as a chelating agent, which helps to bind and remove metal ions from solutions. The solution is commonly used in analytical, biochemical, and industrial processes that require the control or removal of metal ions.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
5
Matrigel by Corning
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Netherlands, Montenegro, Switzerland, Austria, Australia, Colombia, Spain, Morocco, India, Azerbaijan
Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
6
Mtesr1 medium by STEMCELL
Sourced in Canada, United States, France, Germany, United Kingdom, Japan, Italy
MTeSR1 is a complete, serum-free medium designed for the maintenance of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) in an undifferentiated state. It provides the necessary components to support the growth and self-renewal of pluripotent stem cells.
7
Matrigel by BD
Sourced in United States, United Kingdom, Germany, China, Canada, Japan, Italy, France, Belgium, Australia, Uruguay, Switzerland, Israel, India, Spain, Denmark, Morocco, Austria, Brazil, Ireland, Netherlands, Montenegro, Poland
Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
8
Trypsin edta by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, Canada, Switzerland, France, China, Australia, Japan, Italy, Spain, New Zealand, Sweden, Singapore, Portugal, Thailand, Belgium, Denmark, Taiwan, Province of China, Ireland, Brazil, Netherlands, Austria, Czechia, India, Morocco, Israel, Poland, Macao
Trypsin-EDTA is a solution used in cell culture applications to dissociate adherent cells from their growth surface. It contains the proteolytic enzyme trypsin and the chelating agent EDTA, which work together to break down the cellular adhesions and allow the cells to be harvested and passaged.
9
L glutamine by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, France, Canada, Switzerland, Italy, Australia, Belgium, China, Japan, Austria, Spain, Brazil, Israel, Sweden, Ireland, Netherlands, Gabon, Macao, New Zealand, Holy See (Vatican City State), Portugal, Poland, Argentina, Colombia, India, Denmark, Singapore, Panama, Finland, Cameroon
L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
10
Versene solution by PanEco
Sourced in United States
Versene solution is a chelating agent used in laboratory settings. It is a clear, colorless liquid that forms stable complexes with metal ions, allowing for their removal or control in various analytical and industrial applications. The core function of Versene solution is to sequester and bind metal ions, making it a versatile tool for laboratory processes.

More about "Versene"

Versene is an innovative AI-powered protocol comparison tool that revolutionizes the way researchers approach their workflows and identify the best protocols from literature, preprints, and patents.
Powered by advanced AI algorithms, Versene streamlines the research process, enabling users to easily locate and compare relevant protocols across a variety of fields.
With Versene, researchers can experience the power of AI-enhanced research, seamlessly identifying the right products and solutions for their needs.
The platform offers a concise, informative overview of available protocols, including details on Versene solutions, FBS, Penicillin/streptomycin, Matrigel, MTeSR1 medium, and Trypsin-EDTA, providing a valuable resource for scientists.
Versene's AI-driven comparison capabilities allow users to quickly and effortlessly find the optimal protocols, saving time and enhancing efficiency.
Whether you're working with cell culture, molecular biology, or any other research area, Versene can help you streamline your workflow and uncover the best protocols for your specific needs.
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