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Ascorbic Acid

Ascorbic Acid, also known as Vitamin C, is a critical nutrient with diverse biological functions.
It serves as an essential cofactor for numerous enzymes involved in collagen synthesis, neurotransmitter production, and antioxidant defense.
Ascorbic Acid plays a key role in immune function, wound healing, and the prevention of scurvy.
This powerful antioxidant helps protect cells from oxidative damage and supports overall health and wellbeing.
Researchers utilize a variety of methodologies to study the pharmacokinetics, metabolism, and therapeutic applications of Ascorbic Acid.
PubCompare.ai offers a powerful AI-driven tool to optimize this research by identifying the most reproducible and accruate methods from the literature, preprints, and patents.
Boost your Ascorbic Acid research effciency and accuracy with PubCompare.ai.

Most cited protocols related to «Ascorbic Acid»

1
Maintaining Human Embryonic Stem Cells
Human ES cells (H1 and H9) were usually maintained in specific media on Matrigel-coated tissue culture plates32 (link). Cells were passaged routinely with EDTA as described previously13 (link). Briefly, cells were washed twice with PBS/EDTA medium (0.5 mM EDTA in PBS, osmolarity 340 mOsm), then incubated with PBS/EDTA for 5 minutes at 37°C. PBS/EDTA was removed, and cells were washed off swiftly with a small volume of corresponding media.
E8 media composition: Media contained DMEM/F12, L-ascorbic acid-2-phosphate magnesium (64 mg/l), sodium selenium (14 µg/l), FGF2 (100 µg/l), insulin (19.4 mg/l), NaHCO3 (543 mg/l) and transferrin (10.7 mg/l), TGFβ1(2 µg/l) or NODAL (100 µg/l). Osmolarity of all media was adjusted to 340 mOsm at pH7.4. All the media were stored at 4°C, and were used within 2 weeks of production. L-ascorbic acid-2-phosphate magnesium is the stable form of L-ascorbic acid in cell culture.
Chen G., Gulbranson D.R., Hou Z., Bolin J.M., Ruotti V., Probasco M.D., Smuga-Otto K., Howden S.E., Diol N.R., Propson N.E., Wagner R., Lee G.O., Antosiewicz-Bourget J., Teng J.M, & Thomson J.A. (2011). Chemically defined conditions for human iPS cell derivation and culture. Nature methods, 8(5), 424-429.
Publication 2011
Ascorbic Acid Bicarbonate, Sodium Edetic Acid Fibroblast Growth Factor 2 Human Embryonic Stem Cells Insulin L Forms magnesium ascorbate-2-phosphate matrigel Osmolarity Selenium Sodium TGF-beta1 Tissues Transferrin
2
Generating Human iPSC-Derived Neurons for Neuroscience Research
Embryoid bodies were generated from hiPSCs and then transferred to nonadherent plates (Corning). Colonies were maintained in suspension in N2 media (DMEM/F12 (Invitrogen), 1x N2 (Invitrogen)) for 7 days and then plated onto polyornithine (PORN)/Laminin-coated plates. Visible rosettes formed within 1 week and were manually dissected and cultured in NPC media (DMEM/F12, 1x N2, 1x B27-RA (Invitrogen), 1 µg/ml Laminin (Invitrogen) and 20 ng/ml FGF2 (Invitrogen). NPCs are maintained at high density, grown on PORN/Laminin-coated plates in NPC media and split approximately 1:4 every week with Accutase (Millipore). For neural differentiations, NPCs were dissociated with Accutase and plated at low density in neural differentiation media (DMEM/F12-Glutamax, 1x N2, 1x B27-RA, 20 ng/ml BDNF (Peprotech), 20 ng/ml GDNF (Peprotech), 1 mm dibutyrl-cyclicAMP (Sigma), 200 nM ascorbic acid (Sigma) onto PORN/Laminin-coated plates.
Assays for neuronal connectivity, neurite outgrowth, synaptic protein expression, synaptic density, electrophysiology, spontaneous calcium transient imaging and gene expression were used to compare control and SCZD hiPSC neurons.
Additional methods are found in S.I.
Brennand K., Simone A., Jou J., Gelboin-Burkhart C., Tran N., Sangar S., Li Y., Mu Y., Chen G., Yu D., McCarthy S., Sebat J, & Gage F.H. (2011). Modeling schizophrenia using hiPSC neurons. Nature, 473(7346), 221-225.
Publication 2011
accutase Ascorbic Acid Biological Assay Calcium Culture Media Embryoid Bodies Fibroblast Growth Factor 2 GDNF protein, human Gene Expression Human Induced Pluripotent Stem Cells Laminin Nervousness Neuronal Outgrowth Neurons polyornithine Proteins Schizophrenia Transients
3
Generating Inducible Cre Expressing mES Cells
Mouse embryonic stem (ES) cells were cultured on irradiated MEFs in DMEM / 15% FBS, penicillin/streptomycin (P/S, Gibco), 2 mM glutamax (Invitrogen), nonessential amino acids, 0.1 mM β-mercaptoethanol, and 100 U/mL LIF (Peprotech). For EB differentiation, ES cells were trypsinized, and re-plated in differentiation medium (IMDM/15% FBS, 200 μg/mL transferrin (Sigma), 4.5 mM monothiolglycerol (MTG, Sigma), 50 μg/mL ascorbic acid (Sigma), and 2 mM glutamax) for 30 min to allow MEFs to adhere. Nonadherent cells (105) were plated as a cell suspension in low adherence dishes on a slowly rotating shaker. To generate A2Lox.cre ES cells, the HPRT 5′ repair/targeting plasmid [9 (link)] carrying the cassette exchange TRE-2loxP-Δneo inducible target locus [10 ] was digested with XhoI and ligated to an XhoI-SalI fragment bearing the cre transgene from pSalk-cre [11 (link)]. 20 μg of SalI-linearized DNA was electroporated into 6×106 A17 mES cells, and selection in ES medium with HAT supplement (Invitrogen) was initiated 24 hours later.
Iacovino M., Bosnakovski D., Fey H., Rux D., Bajwa G., Mahen E., Mitanoska A., Xu Z, & Kyba M. (2011). Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells. Stem cells (Dayton, Ohio), 29(10), 1580-1588.
Publication 2011
2-Mercaptoethanol Amino Acids Ascorbic Acid Cells Dietary Supplements Embryo Embryonic Stem Cells Hyperostosis, Diffuse Idiopathic Skeletal Mouse Embryonic Stem Cells Penicillins Plasmids Stem, Plant Streptomycin Transferrin Transgenes
4
Validation of a Food Frequency Questionnaire for Pregnant Women
We used a semi-quantitative FFQ of 101 food items to assess the usual daily intake of foods and nutrients (available at: http://bibliodieta.umh.es/files/2011/07/CFA101.pdf). The FFQ was a modified version from a previous FFQ based on the Harvard questionnaire [15 (link)], which we developed and validated using four 1-week dietary records in an adult population in Valencia. The validity correlation coefficients (adjusted for energy intake) ranged from 0.27 for folate intake to 0.67 for calcium intake (average 0.47), and the reproducibility correlation coefficient s ranged from 0.30 for carotene intake to 0.65 for calcium intake (average 0.40) [16 ,17 (link)]; this is a similar range to other established diet questionnaires [3 ,4 (link)]. For the dietary assessment of pregnant women in the INMA cohort study, we added additional food items in the FFQ in order to capture the major sources of the most relevant nutrients, including specific carotenoids.
Participants in the study were asked twice during pregnancy how often, on average, they had consumed each food item over two periods of several months. The first period covered the time from the last menstruation to the first prenatal visit that occurred between the 10–13 weeks of pregnancy; the second period was the time between the first visit and the second one between weeks 28–32 of gestation. Serving sizes were specified for each food item in the FFQ. The questionnaire had nine possible responses, ranging from ‘never or less than once per month’ to ‘six or more per day’. Additionally, we asked whether study participants followed special diets.
Nutrient values were primarily obtained from the food composition tables of the US Department of Agriculture publications as well as other published sources for Spanish foods and portion sizes [18 ,19 ]. In order to obtain average daily nutrient intakes from diet for each individual, we multiplied the frequency of use for each food by the nutrient composition of the portion/serving size specified on the FFQ and added the results across all foods. For those nutrients often used in supplements during pregnancy such as folate, vitamin C and vitamin B12, the total daily nutrient intake was estimated by adding the average daily intake from supplements and the usual daily nutrient intake from the FFQ. In order to convert folic acid intake from supplements to dietary folate, we used the equivalence of 1 mcg of folate in the diet equals to 0.6 mcg of folic acid from supplements [20 (link)]. We estimated the mean daily consumption for 17 foods and food groups by grouping the intake of specific foods in the FFQ (Table 1).
Vioque J., Navarrete-Muñoz E.M., Gimenez-Monzó D., García-de-la-Hera M., Granado F., Young I.S., Ramón R., Ballester F., Murcia M., Rebagliato M, & Iñiguez C. (2013). Reproducibility and validity of a food frequency questionnaire among pregnant women in a Mediterranean area. Nutrition Journal, 12, 26.
Full text: Click here
Publication 2013
A-101 Adult Ascorbic Acid Calcium, Dietary Carotene Carotenoids Cobalamins Diet Dietary Supplements Eating Folate Folic Acid Food Hispanic or Latino Menstruation Nutrient Intake Nutrients Pregnancy Pregnant Women
5
Eligibility Criteria for Glycemic Study
Study subjects were recruited at 11 centers in the U.S., Europe, Africa, and Asia according to the consensus protocol. Type 1 and type 2 diabetic and nondiabetic volunteers were between the ages of 18 and 70 years and were judged as likely to be able to complete the protocol, including performance of the self-monitoring by fingerstick and continuous glucose monitoring. To be eligible, nondiabetic subjects had to have no history of diabetes, a plasma glucose level <97 mg/dl (5.4 mmol/l) after an overnight fast, and an A1C level <6.5%. The diabetic subjects had to have stable glycemic control as evidenced by two A1C values within 1 percentage point of each other in the 6 months before recruitment. Any conditions that might result in a major change in glycemia, such as diseases that might require steroid therapy or plans for pregnancy during the study period, were exclusionary. Similarly, any conditions or treatments that might interfere with the measurement of A1C by any of the study methods, such as hemoglobinopathies (22 (link)), or that might interfere with the putative relationship between A1C and AG values, including anemia (hematocrit <39% in men and <36% in women), high erythrocyte turnover as evidenced by reticulocytosis, blood loss and/or transfusions, chronic renal or liver disease, or high-dose vitamin C or erythropoetin treatment, were grounds for exclusion. The study was approved by the human studies committees at the participating institutions, and informed consent was obtained from all participants.
Nathan D.M., Kuenen J., Borg R., Zheng H., Schoenfeld D, & Heine R.J. (2008). Translating the A1C Assay Into Estimated Average Glucose Values. Diabetes Care, 31(8), 1473-1478.
Publication 2008
Anemia Ascorbic Acid Blood Transfusion Diabetes Mellitus Erythrocytes Glucose Glycemic Control Hemoglobinopathies Hemorrhage Homo sapiens Kidney Liver Diseases Plasma Steroids Volumes, Packed Erythrocyte Voluntary Workers Woman

Most recents protocols related to «Ascorbic Acid»

1
Preservative Silk Gel Formulations
Not available on PMC !

Example 11

FIG. 93 is a table summarizing embodiments of preservative gels of the present disclosure. Silk gels were cast with standard 2% silk solution and 100 mg L-ascorbic acid/15 mL solution with the addition of a preservative and chelating agent. The preservative added was Verstatil SL by Kinetic (Water, Sodium Levulinate, Potassium Sorbate) at 1.5% and the chelating agent was Dermofeel-PA3 by Kinetic (Sodium Phytate) at 0.1%. The addition of preservatives extended gelation time to 7 days. Gel is being observed for discoloration and integrity with L-ascorbic acid and ascorbic acid-2-glucoside gel comparisons.

US11878070B2. Silk-based moisturizer compositions and methods thereof (2024-01-23). Evolved By Nature, Inc. [US]. Inventors: Gregory H. Altman [US], Dylan S. Haas [US], Kevin T. Healy [US].
Full text: Click here
Patent 2024
Ascorbic Acid ascorbic acid 2-O-glucoside CD3EAP protein, human Chelating Agents Kinetics Pharmaceutical Preservatives Silk Sodium Sodium Phytate Sorbate, Potassium
2
Green Tea Infused Silk Gel
Not available on PMC !

Example 10

Steps:
Green Tea PrepHeat 250 mL water to boil
Steep tea bag 2-3 minutes
with occasional stir
remove tea bag and let cool
Gel SolutionUse TFF-10-0047 (3.71% silk)
Prepdilute to 3% silk with water
dilute to 2% with green tea
add L-ascorbic acid
GelGelation occurred like standard
gel at room temperature
Green/yellow color
Green Tea scent
Solution Spec:2% silk solution
65 mL (35 ml of 3.71% silk, 8.3
mL water, 21.66 mL green tea)
0.43 gL-ascorbic acid

FIG. 92 is a table summarizing an embodiment of a caffeine gel of the present disclosure. A silk gel with 2% silk and 100 mg L-ascorbic acid/15 mL solution was created with the addition of 50 mg caffeine/15 mL solution. The gel has the exact appearance of standard L-ascorbic acid gels.

US11857663B2. Stable silk protein fragment compositions (2024-01-02). Evolved By Nature, Inc. [US]. Inventors: Gregory H. Altman [US], Rebecca L. Lacouture [US], Rachel Lee Dow [US], Rachel M. Lind [US], Dylan S. Haas [US].
Full text: Click here
Patent 2024
Ascorbic Acid Caffeine Fast Green FCF Furuncles Gels Green Tea Pheromone Silk STEEP1 protein, human Technique, Dilution
3
Green Tea and Silk Nanocomposite Gel
Not available on PMC !

Example 10

Steps:

    • Green Tea Prep Heat 250 mL water to boil
      • Steep tea bag 2-3 minutes with occasional
      • stir
      • remove tea bag and let cool
    • Gel Solution
    • Prep Use TFF-10-0047 (3.71% silk)
      • dilute to 3% silk with water
      • dilute to 2% with green tea
      • add L-ascorbic acid
      • Gelation occurred like standard gel at room
    • Gel temperature
      • Green/yellow color
      • Green Tea scent
    • Solution Spec: 2% silk solution
      • 65 mL (35 ml of 3.71% silk, 8.3 mL water,
      • 21.66 mL green tea)
      • 0.43 g L-ascorbic acid

FIG. 92 is a table summarizing an embodiment of a caffeine gel of the present disclosure. A silk gel with 2% silk and 100 mg L-ascorbic acid/15 mL solution was created with the addition of 50 mg caffeine/15 mL solution. The gel has the exact appearance of standard L-ascorbic acid gels.

US11857664B2. Stable silk protein fragment compositions (2024-01-02). Evolved By Nature, Inc. [US]. Inventors: Gregory H. Altman [US], Rebecca L. Lacouture [US], Rachel Lee Dow [US], Rachel M. Lind [US], Dylan S. Haas [US].
Full text: Click here
Patent 2024
Ascorbic Acid Caffeine Fast Green FCF Furuncles Gels Green Tea Pheromone Silk STEEP1 protein, human Technique, Dilution
4
Direct Mass Spectrometry Imaging of Plant Tissues
Not available on PMC !

Example 8

FIGS. 12A-D show] direct MS spectra of plant tissues using sliced tissues of four kinds of plants. (FIG. 12A) Onion, (FIG. 12B) Spring onion, and two different leaves (FIG. 12C) and (FIG. 12D).

FIGS. 13A and 13B show an MS/MS spectra of Vitamin C analysis (FIG. 13A) direct analysis of onion without sample preparation, (FIG. 13B) using standard solution.

US11867684B2. Sample dispenser including an internal standard and methods of use thereof (2024-01-09). Purdue Research Foundation [US]. Inventors: Zheng Ouyang [US], He Wang [US], Nicholas E. Manicke [US], Robert Graham Cooks [US], Qian Yang [US], Jiangjiang Liu [US].
Full text: Click here
Patent 2024
Allium cepa Ascorbic Acid Plants Spectrum Analysis Tandem Mass Spectrometry Tissues
5
Silicic Acid and Micronutrients for Health
Not available on PMC !

Example 5

    • Daily oral administration of 5 mg of bioavailable silicic acid in the form of choline-stabilized orthosilicic acid (ch-OSA®), wherein silicic acid is stabilized with choline chloride, for instance in the form of a capsule.
    • Daily administration of a tablet containing 200 mg vitamin C, 150 microgram selenium, 10 mg zinc, 1 mg copper.

US11878031B2. Silicic acids for use in the treatment of periodontitis (2024-01-23). Bio Minerals N.V. [BE]. Inventors: Mario Remi Yvonne Calomme [BE], Kathleen Jozef Ingrid Suzanne Van Hoof [BE].
Full text: Click here
Patent 2024
Acids Administration, Oral Ascorbic Acid Capsule Choline Choline Chloride Copper Periodontitis Selenium Silicic acid Tablet Zinc

Top products related to «Ascorbic Acid»

1
Ascorbic acid by Merck Group
Sourced in United States, Germany, United Kingdom, France, Italy, India, China, Sao Tome and Principe, Canada, Spain, Macao, Australia, Japan, Portugal, Hungary, Brazil, Singapore, Switzerland, Poland, Belgium, Ireland, Austria, Mexico, Israel, Sweden, Indonesia, Chile, Saudi Arabia, New Zealand, Gabon, Czechia, Malaysia
Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
2
Dexamethasone (dex) by Merck Group
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
3
L ascorbic acid by Merck Group
Sourced in United States, Germany, United Kingdom, China, Italy, Australia, India, Macao, France, Spain, Poland, Sao Tome and Principe, Japan, Singapore, Hungary, Ireland, Netherlands, Canada, Mexico, Czechia, Sweden, Denmark, Switzerland
L-ascorbic acid is a chemical compound commonly known as vitamin C. It is a white, crystalline solid that is soluble in water and has a slight acidic taste. L-ascorbic acid is an essential nutrient required for various metabolic processes in the body and acts as an antioxidant, protecting cells from damage caused by free radicals.
4
β glycerophosphate by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Italy, China, Sao Tome and Principe, France, Canada, Australia, Macao, India, Senegal, Ireland, Spain, Denmark, Belgium
β-glycerophosphate is a chemical compound that serves as a buffering agent and source of phosphate for cell culture media. It helps maintain a stable pH environment for cell growth and proliferation.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
2 2 diphenyl 1 picrylhydrazyl (dpph) by Merck Group
Sourced in United States, Germany, Italy, India, China, Spain, Poland, France, United Kingdom, Australia, Brazil, Singapore, Switzerland, Hungary, Mexico, Japan, Denmark, Sao Tome and Principe, Chile, Malaysia, Argentina, Belgium, Cameroon, Canada, Ireland, Portugal, Israel, Romania, Czechia, Macao, Indonesia
DPPH is a chemical compound used as a free radical scavenger in various analytical techniques. It is commonly used to assess the antioxidant activity of substances. The core function of DPPH is to serve as a stable free radical that can be reduced, resulting in a color change that can be measured spectrophotometrically.
7
Gallic acid by Merck Group
Sourced in United States, Germany, Italy, Spain, France, India, China, Poland, Australia, United Kingdom, Sao Tome and Principe, Brazil, Chile, Ireland, Canada, Singapore, Switzerland, Malaysia, Portugal, Mexico, Hungary, New Zealand, Belgium, Czechia, Macao, Hong Kong, Sweden, Argentina, Cameroon, Japan, Slovakia, Serbia
Gallic acid is a naturally occurring organic compound that can be used as a laboratory reagent. It is a white to light tan crystalline solid with the chemical formula C6H2(OH)3COOH. Gallic acid is commonly used in various analytical and research applications.
8
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
9
Quercetin by Merck Group
Sourced in United States, Germany, Italy, India, Spain, United Kingdom, France, Poland, China, Sao Tome and Principe, Australia, Brazil, Macao, Switzerland, Canada, Chile, Japan, Singapore, Ireland, Mexico, Portugal, Sweden, Malaysia, Hungary
Quercetin is a natural compound found in various plants, including fruits and vegetables. It is a type of flavonoid with antioxidant properties. Quercetin is often used as a reference standard in analytical procedures and research applications.
10
Methanol by Merck Group
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Methanol is a clear, colorless, and flammable liquid that is widely used in various industrial and laboratory applications. It serves as a solvent, fuel, and chemical intermediate. Methanol has a simple chemical formula of CH3OH and a boiling point of 64.7°C. It is a versatile compound that is widely used in the production of other chemicals, as well as in the fuel industry.

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