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Pantothenate, Calcium

Pantothenate, Calcium is a salt form of pantothenic acid, a B-complex vitamin essential for energy metabolism, fatty acid synthesis, and the production of red blood cells.
It plays a crucial role in the body's ability to convert food into cellular energy.
Calcium pantothenate is commonly used as a dietary supplement to support overall health and wellness.
This compound can be found naturally in a variety of foods, including meat, dairy, legumes, and whole grains.
Reasearchers continue to explore the potential benefits of Calcium Pantothenate supplementatioin for conditions such as stress, fatigue, and immune function.

Most cited protocols related to «Pantothenate, Calcium»

The 3T3-L1 cell (#EC86052701-G0, KAK) is a universally used cell line for lipid studies. The 3T3-L1 preadipocytes were grown until confluence at 37 °C in HG- DMEM containing 8 mg/L d-biotin, 4 mg/L calcium pantothenate, 100 U/mL penicillin, 100 μg/mL streptomycin (b.p. HG-DMEM), and 10% CS.
The 3T3-L1 organoids were generated by a hanging droplet spheroid three dimension (3D) culture system as described recently28 (link). Briefly, to facilitate stable morphology, methylcellulose (Methocel A4M) was added to the growth medium. Prior to seeding the hanging drop culture plate (# HDP1385, Sigma-Aldrich), cells were cultured in 100 mm or 150 mm dishes until reaching approximately 90% confluence. After washing with a phosphate buffered saline (PBS), cells were detached using 0.25% Trypsin/EDTA and resuspended in growth medium. After centrifugation for 5 min at 300 g, the cell pellet was re-suspended in a growth medium containing 0.25% w/v Methocel A4M. Volume was adjusted so that 20,000 cells were contained in the 28 μL solution, and 28 μL drops were placed into each well of the drop culture plate (defined as 3D/Day 0). An organoid medium (i.e. growth medium with 0.25% w/v Methocel A4M) was used throughout the duration of the spheroid culture. On every following day, 14 μL of the culture medium was removed and a fresh 14 μL culture medium was added to each well.
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Publication 2020
3T3-L1 Cells Biotin Cell Lines Cells Centrifugation Culture Media Edetic Acid Hyperostosis, Diffuse Idiopathic Skeletal Lipid A Methocel Methocel E Methylcellulose Organoids Pantothenate, Calcium Penicillins Phosphates Saline Solution Streptomycin Trypsin
Continuous cultures were established using published methods [54] (link) with the exception of the phosphate-limited media, which contained the following (per liter): 100 mg calcium chloride, 100 mg sodium chloride, 500 mg magnesium sulfate, 5 g ammonium sulfate, 1 g potassium chloride, 500 µg boric acid, 40 µg copper sulfate, 100 µg potassium iodide, 200 µg ferric chloride, 400 µg manganese sulfate, 200 µg sodium molybdate, 400 µg zinc sulfate, 1 µg biotin, 200 µg calcium pantothenate, 1 µg folic acid, 1 mg inositol, 200 µg niacin, 100 µg p-aminobenzoic acid, 200 µg pyridoxine, 100 µg riboflavin, 200 µg thiamine, 10 mg potassium phosphate, and 5 g glucose.
Experiments were started by initially growing cultures in 300mL of the appropriate defined media in batch phase. Once the cultures reached saturation, chemostat flow was initiated. Cultures were grown at a dilution rate of 0.17 volumes/hour. Daily samples were taken from the overflow in order to determine optical density at 600 nm, cell count and viability; perform microscopy; and make archival glycerol stocks. We confirmed that all evolved haploid clones maintained the same mating type as the founder by backcrossing the evolved strain to the isogenic ancestral strain of the opposite mating type. Clones from three of the twelve evolved diploid populations exhibited reduced sporulation efficiency, but did not mate inappropriately.
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Publication 2008
4-Aminobenzoic Acid Biotin boric acid Calcium chloride Clone Cells Diploidy ferric chloride Folic Acid Glucose Glycerin Inositol manganese sulfate Microscopy Niacin Pantothenate, Calcium Phosphates Population Group Potassium Chloride Potassium Iodide potassium phosphate Pyridoxine Riboflavin Sodium Chloride sodium molybdate(VI) Strains Sulfate, Ammonium Sulfate, Copper Sulfate, Magnesium Technique, Dilution Thiamine Vision Zinc Sulfate
The 3T3-L1 preadipocytes (#EC86052701-G0, KAK), a cell line that is universally used in lipid studies, were cultured in 2D culture dishes at 37 °C in a grown medium; high glucose Dulbecco’s modified Eagle medium (HG-DMEM, FUJIFILM, Osaka, Japan) supplemented with 8 mg/L d-biotin, 4 mg/L calcium pantothenate, 100 U/mL penicillin, 100 μg/mL streptomycin (b.p. HG-DMEM), 10% CS and methylcellulose (Methocel A4M, Sigma-Aldrich, St. Louis, MO, USA) as a morphology stabilizer.
To generate 3D spheroids under culture conditions identical to those used for the 2D cell culture except culture plates, a hanging droplet spheroid 3D culture system, described recently [6 (link),14 (link)], was used. Briefly, 3T3-L1 cells were cultured in 100 mm or 150 mm dishes as described above. After reaching approximately 90% confluence, the cells were detached by treatment with 0.25% trypsin/EDTA after washing with phosphate-buffered saline (PBS) and then resuspended in the growth medium. These cell suspensions were divided into conventional 2D cultures and 3D spheroid cultures. The 2D-cultured 3T3-L1 cells were again maintained 100 mm 2D culture dishes until Day 7, with medium changes daily. Alternatively, a cell suspension containing approximately 20,000 cells in a 28 μL culture medium was then placed into each well of the 3D drop culture plate (# HDP1385, Sigma-Aldrich, St. Louis, MO, USA). This timing was defined as 3D/Day 0, and 14 μL of the culture medium was then replaced with 14 μL of fresh culture medium in each well daily until Day 7 [7 (link),8 (link),10 (link)]. On Day 7, both 2D- and 3D-cultured 3T3-L1 cells were each collected and further processed for use in the RNA-sequencing analysis, as described below.
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Publication 2022
3T3-L1 Cells Biotin Cell Culture Techniques Cell Lines Cells Culture Media Eagle Edetic Acid Glucose Hyperostosis, Diffuse Idiopathic Skeletal Lipids Methocel Methylcellulose Pantothenate, Calcium Penicillins Phosphates Saline Solution Sequence Analysis Streptomycin Trypsin
The 3T3-L1 preadipocytes (#EC86052701-G0, KAK) were grown in 2D culture medium (HG-DMEM containing 100 U/mL penicillin, 100 μg/mL streptomycin and 10% CS) in 150 mm dish until confluence at 37℃.
To obtain 3T3-L1 organoids, 3T3-L1 preadipocytes were grown in 3D pre-culture medium (HG-DMEM containing 8 mg/L d-biotin, 4 mg/L calcium pantothenate, 100 U/mL penicillin, 100 μg/mL streptomycin and 10% CS) in 150 mm dish as above. When the cultures reached approximately a 90% confluence, the cells were washed with phosphate buffered saline (PBS), detached using 0.25% Trypsin/EDTA and resuspended in 3D pre-culture medium containing 0.25% w/v Methocel A4M (3D organoid medium). Approximately 20,000 cells in the 28μL 3D organoid medium were placed into each well of the drop culture plate (defined as 3D/Day 0). On alternate days, 14 μL of the medium was substituted with 14 μL of fresh medium in each well.
For the induction of adipogenic differentiation, two days after the 2D cells in the 2D culture medium or 3D organoids at Day 1 in the 3D organoid medium reached confluence, there were processed by supplementation with 250 nM dexamethasone, 10 nM T3, 10 μM troglitazone, and 1 μg/ml insulin during the initial two days, and during the following 4 days with 10 μM troglitazone and 1 μg/ml insulin. To study the efficacy of several drugs, 100 nM prostaglandin F2α (PGF2α), 100 nM omidenepag (OMD), or 100 nM butaprost (Buta) were added during their adipogenic differentiation. In terms of their corresponding receptor, PF and EP2 receptors, of these drugs, we confirmed their positive expressions with the 2D and 3D cultured 3T3-L1 cells (Supplemental Fig. 1).
Phase contrast images of the 3D organoids were captured in a × 4 objective lens using an inverted microscope (Nikon ECLIPSE TS2; Tokyo, Japan). For measurement of each organoid size, the largest cross-sectional area (CSA) was calculated using the Image-J software version 1.51n (National Institutes of Health, Bethesda, MD).
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Publication 2020
3T3-L1 Cells Adipogenesis Biotin butaprost Cells Dexamethasone Dinoprost Edetic Acid Hyperostosis, Diffuse Idiopathic Skeletal Insulin Lens, Crystalline Methocel Microscopy Microscopy, Phase-Contrast Organoids Pantothenate, Calcium Penicillins Pharmaceutical Preparations Phosphates Saline Solution Streptomycin Troglitazone Trypsin
All pneumococcal strains used in this study are derivatives of the clinical isolate S. pneumoniae D39V (Avery et al., 1944 (link), Slager et al., 2018 (link)) unless specified otherwise. See Table S6 for a list of the strains used and the Supplemental information for details on the construction of the strains.
S. pneumoniae was grown in C+Y medium at 37°C. C+Y was adapted from Adams and Roe (Adams and Roe 1945 (link)) and contained the following compounds: adenosine (68.2 μM), uridine (74.6 μM), L-asparagine (302 μM), L-cysteine (84.6 μM), L-glutamine (137 μM), L-tryptophan (26.8 μM), casein hydrolysate (4.56 g L-1), BSA (729 mg L-1), biotin (2.24 μM), nicotinic acid (4.44 μM), pyridoxine (3.10 μM), calcium pantothenate (4.59 μM), thiamin (1.73 μM), riboflavin (0.678 μM), choline (43.7 μM), CaCl2 (103 μM), K2HPO4 (44.5 mM), MgCl2 (2.24 mM), FeSO4 (1.64 μM), CuSO4 (1.82 μM), ZnSO4 (1.58 μM), MnCl2 (1.29 μM), glucose (10.1 mM), sodium pyruvate (2.48 mM), saccharose (861 μM), sodium acetate (22.2 mM) and yeast extract (2.28 g L-1).
We can control competence development by changing the pH in the medium. The underlying mechanism it is not fully understood, but it is believed that is related to the production and export of CSP (Moreno-Gámez et al., 2017 (link)). For this reason, we always grow a preculture in C+Y at pH 6.8, because at this pH, even the hypercompetent strains such as ΔlytB or Δpbp3 mutants, are not able to accumulate enough CSP to induce competence before cells reach stationary phase.
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Publication 2018
Adenosine Asparagine Biotin casein hydrolysate Cells Choline Cysteine derivatives Glucose Glutamine Magnesium Chloride manganese chloride Nicotinic Acids Pantothenate, Calcium potassium phosphate, dibasic Pulmonal S Pyridoxine Pyruvate Riboflavin Sodium Sodium Acetate Strains Streptococcus pneumoniae Sucrose Thiamine Tryptophan Uridine Yeast, Dried

Most recents protocols related to «Pantothenate, Calcium»

Not available on PMC !

Example 2

100 mg of the Sarcodon aspratus extracts according to the present invention;

an appropriate amount of a vitamin mixture;

70 μg of vitamin A acetate;

1.0 mg of vitamin E;

0.13 mg of vitamin B1;

0.15 mg of vitamin B2;

0.5 mg of vitamin B6;

0.2 μg of vitamin B12;

10 mg of vitamin C;

10 μg of biotin;

1.7 mg of nicotinic acid amide;

50 μg of folate;

0.5 mg of calcium pantothenate;

an appropriate amount of a mineral mixture;

1.75 mg of ferrous sulfide;

0.82 mg of zinc oxide;

25.3 mg of magnesium carbonate;

15 mg of potassium phosphate monobasic;

55 mg of dicalcium phosphate;

90 mg of potassium citrate;

100 mg of calcium carbonate; and

24.8 mg of magnesium chloride.

The composition ratio of the vitamins and the mineral mixture described above may be determined according to a composition ratio used in general functional health foods, and the combination ratio of the vitamins and the mineral mixture may be arbitrarily determined. According to a conventional method of preparing functional health foods, these components are mixed, granules are prepared, and the granules are used to prepare a composition for a functional health food.

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Patent 2024
Ascorbic Acid Biotin Carbonate, Calcium Cobalamins Cytoplasmic Granules dicalcium phosphate ferrous sulfide Folate Functional Food magnesium carbonate Magnesium Chloride magnesium citrate Minerals Niacinamide Pantothenate, Calcium Potassium Potassium Citrate potassium phosphate retinol acetate Riboflavin Sarcodon aspratus Thiamine Vitamin A Vitamin B6 Vitamin E Vitamins Zinc Oxide

Example 3

Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 μm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.

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Patent 2024
Clostridium histolyticum Fermentation ferrous sulfate Filtration Niacin Pantothenate, Calcium Pimelic Acid Pyridoxine Riboflavin Sterilization Strains Sulfate, Magnesium Thiamine Vitamins

Example 3

Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 pm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.

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Patent 2024
Clostridium histolyticum Fermentation ferrous sulfate Filtration Niacin Pantothenate, Calcium Pimelic Acid Pyridoxine Riboflavin Sterilization Strains Sulfate, Magnesium Thiamine Vitamins
E. coli Trans 5α (TransGen Biotech, Beijing, China) was used for propagation of plasmids. Luria–Bertani (LB) broth medium (5 g/L yeast extract, 10 g/L tryptone, 10 g/L NaCl) containing 50 mg/L of kanamycin was used to culture E. coli carrying transformed plasmids. S. cerevisiae strain BY4742 (MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0) was used as the parent strain for resveratrol biosynthesis. YPD medium (10 g/L yeast extract, 20 g/L peptone, and 20 g/L glucose, also marked as YPD-20G when needed) was used for routine cultivation of S. cerevisiae strains. YPD medium with 40 g/L glucose was also used for fermentation in shake flasks and marked as YPD-40G. Geneticin (G418, 100 mg/L) was supplemented in the YPD agar plate for the selection of engineered yeast strains edited by CRISPR/Cas9. SC agar plates (synthetic complete drop-out medium, 20 g/L glucose, 6.7 g/L yeast nitrogen base without amino acids, and 0.8 g/L dropout powder minus appropriate amino acids) was used for the selection of engineered yeast strains edited by homologous recombination using HIS3, LEU2, URA3 and LYS2 respectively as selective markers. Minimal medium used in our study was based on studies described previously [36 (link)]. The medium contained 20 g/L or 40 g/L glucose, 15 g/L (NH4)2SO4, 8 g/L KH2PO4, 6.2 g/L MgSO4∙7H2O, 1.2% (v/v) vitamin solution, and 1% (v/v) trace metal solution. L-lysine (0.5 g/L) was added in minimal medium for the cultivation of strain BRT8 and BRT9. The trace metal solution contained 5.75 g/L ZnSO4∙7H2O, 0.32 g/L MnCl2∙4H2O, 0.47 g/L CoCl2∙6H2O, 0.48 g/L Na2MoO4∙2H2O, 2.9 g/L CaCl2∙2H2O, 2.8 g/L FeSO4∙7H2O and 80 mL 0.5 M EDTA, pH 8.0. The vitamin solution contained 0.05 g/L biotin, 1 g/L calcium pantothenate, 1 g/L nicotinic acid, 25 g/L myo-inositol, 1 g/L thiamine HCl, 1 g/L pyridoxal HCl and 0.2 g/L p-aminobenzoic acid.
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Publication 2023
4-Aminobenzoic Acid Agar Amino Acids Amino Acids, Basic Anabolism antibiotic G 418 Biotin Clustered Regularly Interspaced Short Palindromic Repeats Edetic Acid Escherichia coli Fermentation Geneticin Glucose Homologous Recombination Inositol Kanamycin Lysine manganese chloride Metals Nicotinic Acids Nitrogen Pantothenate, Calcium Parent Peptones Plasmids Powder Pyridoxal Resveratrol Saccharomyces cerevisiae Sodium Chloride sodium molybdate(VI) Strains Sulfate, Magnesium thiamine hydrochloride Tremor Vitamins
The thermogenic supplement treatment and placebo were in powder form with uniform scoop sizes and dissolved in 300 mL of cold water. Lab staff prepared the powder and water mixture to mix appropriately and observed the participants’ consumption of the treatments, which had to be completed in <5 min. The ingredients in the active treatment, which contains 150 mg of caffeine (OxyShred Thermogenic Fat Burner, EHP Labs, Salt Lake City, Utah, USA) are presented in Table 1, while the placebo contained only inactive ingredients (gum Arabic, citric acid, malic acid, NAT Watermelon Type, NAT bitter blocker, sucralose, silicon dioxide, calcium silicate, beet color powder). Treatment and placebo powders were blinded for taste, texture, and appearance, produced by the manufacturer, and arrived in blinded containers. All containers were kept at room temperature in a cool and dry location. The treatment was given to the participants after completion of all baseline testing and questionnaires.

EHP Labs OxyShred thermogenic fat burner ingredients list

OxyShred (one serving)Amount/serving% DV
Calories5 
Total carbohydrate1.0 g<1
Dietary fiber0.2 g4*
Vitamin C173 mg193
Thiamin0.56 mg46
Riboflavin0.78 mg60
Niacin20 mg123
Vitamin B60.98 mg58
Vitamin B120.9 mcg38
Pantothenic acid1.7 mg34
Chromium picolinate10 mcg3
Fat burning matrixAcetyl L-carnitine HCl, Garcinia cambogia fruit extract (60% hydroxycitric acid), conjugated linoleic acid (CLA), grapefruit seed extract 4:1, raspberry ketones (from raspberry fruit extract), Mangifera indica seed extract, bitter orange fruit extract, green coffee bean extract (50% chlorogenic acid), olive leaf extract (10% oleuropein), guggul extract powder, chromium picolinate2003 mg 
Immunity booster & prebiotic complexL-glutamine, inulin fiber, vitamin c (ascorbic acid)625 mg 
Mood enhancer matrixL-tyrosine, taurine, caffeine anhydrous (150 mg), Huperzia serrata whole herb extract (Huperzine A)851 mg 
Full B vitamin spectrumNiacinamide (niacin), calcium pantothenate (pantothenic acid), pyridoxine HCl (vitamin B6), riboflavin (vitamin B2), thiamine mononitrate (vitamin B1), cyanocobalamin (vitamin B12)24.59 mg 
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Publication 2023
4-(p-hydroxyphenyl)-2-butanone ARID1A protein, human Ascorbic Acid Beta vulgaris Caffeine calcium silicate Carnitine Chlorogenic Acid Chromium Citric Acid Cobalamins Coffee Cold Temperature Dietary Supplements Excipients Fibrosis Fruit Garcinia cambogia Glutamine grapefruit seed extract gugulu extract Gum Arabic Huperzia huperzine A hydroxycitric acid Inulin Linoleic Acids, Conjugated malic acid Mangifera indica bark Mood Niacin oleuropein olive leaf extract Pantothenate, Calcium Pantothenic Acid Placebos Powder Prebiotics Pyridoxine Hydrochloride Raspberries Response, Immune Riboflavin Secondary Immunization Silicon Dioxide Sodium Chloride sucralose Taste Taurine Thermogenesis Thiamine Thiamine Mononitrate Tyrosine Vitamin B6 Vitamins Watermelon

Top products related to «Pantothenate, Calcium»

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Calcium pantothenate is a chemical compound that is the calcium salt of pantothenic acid, also known as vitamin B5. It is a water-soluble vitamin that plays a crucial role in various metabolic processes within the body. As a lab equipment product, calcium pantothenate is used as a nutrient supplement or as a component in cell culture media for cell growth and maintenance.
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Tween 80 is a non-ionic surfactant and emulsifier. It is a viscous, yellow liquid that is commonly used in laboratory settings to solubilize and stabilize various compounds and formulations.
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Nicotinic acid, also known as niacin, is a water-soluble vitamin that plays a vital role in various metabolic processes within the human body. It serves as a precursor to the important coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), which are essential for cellular energy production, DNA repair, and other biochemical reactions.
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L-leucine is an amino acid that can be used as a laboratory reagent. It serves as a building block for proteins and is commonly used in cell culture media and other biochemical applications.
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Biotin is a laboratory-grade product manufactured by Merck Group. It is a water-soluble vitamin that serves as a cofactor for certain enzymes involved in carboxylation reactions. Biotin is commonly used in various biochemical and molecular biology applications.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Calcium pantothenate is a chemical compound that serves as a component in laboratory equipment. It is a salt of pantothenic acid, also known as vitamin B5. Calcium pantothenate is used in various applications within the scientific and research fields.
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
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Dexamethasone is a synthetic glucocorticoid medication used in laboratory and research settings. It is a potent anti-inflammatory and immunosuppressive agent. Dexamethasone is commonly used in experimental studies to investigate inflammatory processes and immune system responses.
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Optima LC-MS grade methanol is a high-purity solvent designed for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is produced to meet the stringent requirements of LC-MS analysis, ensuring minimal interference and optimized performance.

More about "Pantothenate, Calcium"

Pantothenic Acid, Calcium Pantothenate, B5 Vitamin, Coenzyme A, Energy Metabolism, Fatty Acid Synthesis, Red Blood Cell Production, Dietary Supplement, Stress, Fatigue, Immune Function.
Tween 80, a non-ionic surfactant, can be used alongside Calcium Pantothenate for improved solubility and bioavailability.
Nicotinic Acid, or Vitamin B3, and L-Leucine are other B-complex vitamins that work synergistically with Calcium Pantothenate to support energy levels and overall health.
Biotin, also known as Vitamin B7, is another important cofactor in metabolic processes.
Solvents like Acetonitrile and DMSO can be utilized for Calcium Pantothenate extraction and analysis, while Dexamethasone, a synthetic glucocorticoid, may interact with Calcium Pantothenate supplementation.
Optima LC-MS grade methanol provides high purity for liquid chromatography-mass spectrometry quantification of Calcium Pantothenate.
Whether you're studying the biochemistry, pharmacology, or nutritional aspects of this essential nutrient, PubCompare.ai can help you find the best protocols and optimize your Pantothenate, Calcium research.