Pantothenic Acid
It is involved in the synthesis of coenzyme A, a key molecule in the citric acid cycle and fatty acid metabolism.
Pantothenic Acid is widely distributed in plant and animal tissues, and its deficiency can lead to symptoms such as fatigue, irritability, and digestive issues.
Researchers are actively exploring the potential therapeutic applications of Pantothenic Aicid, including its effects on cardiovascular health, cognitive function, and skin health.
PubCompare.ai's AI-driven platform can help streamline your Pantothenic Acid research by providing access to the best protocols from literature, preprints, and patents, enabling you to identify the most effective approaches and unlock new insights.
Most cited protocols related to «Pantothenic Acid»
Tissue explants were minced and placed directly in plastic culture dishes in DMEM containing 20% FBS, penicillin (100 U/mL), and gentamycin (20 µg/mL), allowing preadipocyte fibroblasts to proliferate. After fibroblasts had grown out from the explants, monolayers were passaged serially by gently treating with trypsin/EDTA, and cultures were maintained in 80-mm flasks containing DMEM with 10% FBS and antibiotics. Cell cultures were grown in a humidified 5% CO2 incubator at 37°C. The strains were stored in liquid N2 until needed, and they were used between the third and seventh passage.
After cells reached confluence in 6-well plates, differentiation of adipocytes was initiated by the following protocol. The culture medium were changed to serum-free DMEM supplemented with 33 µM biotin, 17 µM pantothenic acid, 10 µg/ml transferrin, 0.2 nM T3, 1 µM insulin (Boehringer-Mannheim, Mannheim, Germany), and 0.2 µM carbaprostaglandin (cPGI2; Calbiochem, La Jolla, CA, USA). For the first 4 days, 1 µM insulin, 1 µM dexamethasone, and 0.1 mM isobutylmethylxanthine were included in the media. The differentiation was continued for 10 days, during which the media was replaced every 3 days. A PPARγ agonist, rosiglitazone (10 µM, Cayman, Ann Arbor, MI, USA), was added from day 1 for further stimulation of adipogenesis.
For experiments, the overnight cultures were either diluted 200-fold in LB medium and grown to mid-log phase in a shaking water bath at 200 rpm and 37°C or diluted and used as stationary phase cultures. When necessary, cells were thoroughly washed with PBS to remove traces of medium and resuspended to OD600nm = 0.5 or 0.1 in PBS containing 0.2% glucose. Experiments were performed in 96-well plates. Aliquots (100 μl/well) of cell suspensions were transferred into triplicate wells, and tested compounds were added. Stock solutions of tested compounds were prepared in PBS and filter-sterilized.
M9CA medium consisted of M9 salts, prepared by dissolving 6 g Na2HPO4, 3 g K2HPO4, 1 g NH4Cl, and 0.5 g NaCl in 1 liter of distilled water. To 100 ml of M9 salts, 1 ml of each 0.2 M MgSO4, 20% glucose, casamino acids (Difco) to 20%, and 50 μL of 0.2 M CaCl2, autoclaved separately were added. Immediately before use, filter-sterilized solutions of pantothenic acid and thiamine were added to a final concentration of 3 mg/L.
Individual amino acids (Sigma-Aldrich) were added to phosphate-buffered saline (PBS) (Thermo Fisher Scientific) supplemented with 0.2% glucose or to M9 salts supplemented with 0.2% glucose. Since both media produced similar results, only data obtained in PBS/glucose are presented.
For anaerobic experiments, solutions were degassed, and together with plates containing cell suspensions, were transferred and equilibrated in an anaerobic Coy chamber (Plas Labs Inc., USA). All additions were performed under anaerobic conditions. Anaerobic cultures were grown in a Coy chamber at 37 oC.
Most recents protocols related to «Pantothenic Acid»
Example 6
This example provides a representative example of an aqueous solution to be used in a method described herein. The composition may contain the following ingredients:
The aqueous solution is formulated in physiological saline and adjusted to about pH 7.4, thereby minimizing any injecting pain beyond the needle prick. In addition, the aqueous solution may be optionally supplemented with a preservative (e.g., benzyl alcohol), a chemical stabilizer (e.g., gentisic acid), and/or an additional bioactive agent (e.g., platelet-rich plasma) depending on anticipated delivery method, shelf-life, and intended effects.
Example 7
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) twice a day for a period of 29 days. At the end of the 29 day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
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- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
Example 8
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) on days 1, 3, 7, 10, 14, 21, 30, 60, and 90 of treatment. At the end of the 90-day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
-
- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
While preferred embodiments have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the embodiments. It should be understood that various alternatives to the embodiments described herein may be employed. It is intended that the following claims define the scope of the embodiments and that methods and structures within the scope of these claims and their equivalents be covered thereby.
Example 5
An aqueous solution containing the following ingredients:
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- about 1500 to about 6250 meg cobalamin (vitamin B12);
- about 150 to about 250 mg ascorbic acid (vitamin C);
- about 30 to about 50 mg nicotinamide (vitamin B3);
- about 4.5 to about 7.5 mg thiamine (vitamin B1);
- about 0.1 to about 0.3 mg pyridoxine HCl (vitamin B6);
- about 2.7 to about 4.5 mg riboflavin 5-phosphate sodium (vitamin B2);
- about 7.5 to about 15 mg pantothenic acid (vitamin B5);
- about 0.08 to about 0.125 mg Zinc sulfate heptahydrate; and
- about 1 ml q.s., sterile water for injection
The aqueous solution is formulated in physiological saline and adjusted an acceptable pH in the range of about 6.5 to about 7.5 to 7.4, thereby minimizing any injecting pain beyond the needle prick. The aqueous solution may be optionally supplemented with a preservative (e.g., from about 0.01% to about 2% benzyl alcohol), a chemical stabilizer (e.g., from about 0.01% to about 2% gentisic acid), and/or an additional bioactive agent (e.g., from about 0.01% to about 2% hyaluronic acid) depending on anticipated delivery method, shelf-life, and intended effects.
Composition and nutrient concentrations of basal diet (%, unless noted, as-is basis)
Item | Low phosphorus | Regular phosphorus |
---|---|---|
Ingredients | ||
Corn | 56.69 | 56.69 |
Soybean meal | 25.77 | 25.77 |
Distillers dried grains with solubles | 4.00 | 4.00 |
Calcium carbonate | 9.73 | 9.04 |
Dicalcium phosphate | - | 1.15 |
Soybean oil | 1.51 | 1.51 |
Sodium chloride | 0.26 | 0.26 |
DL-Methionine | 0.18 | 0.18 |
Choline chloride | 0.15 | 0.15 |
Montmorillonite | 0.71 | 0.25 |
Premix1 | 1 | 1 |
In total | 100.00 | 100.00 |
Nutrient levels | ||
Metabolizable energy, kcal/kg (calculated) | 2,600 | 2,600 |
Crude protein (calculated) | 16.5 | 16.5 |
Total phosphorus (calculated/analyzed) | 0.34/0.34 | 0.53/0.49 |
Non-phytate phosphorus (calculated) | 0.14 | 0.32 |
Calcium (calculated/analyzed) | 3.50/3.47 | 3.50/3.52 |
1Provided per kilogram of diet: manganese 60 mg, copper 8 mg, zinc 80 mg, iodine 0.35 mg, selenium 0.3 mg, vitamin A 8000 IU, vitamin E 30 mg, vitamin K3 1.5 mg, thiamine 4 mg, riboflavin 13 mg, pantothenic acid 15 mg, nicotinamide 20 mg, pyridoxine 6 mg, biotin 0.15 mg, folic acid 1.5 mg, and cobalamin 0.02 mg
EHP Labs OxyShred thermogenic fat burner ingredients list
OxyShred (one serving) | Amount/serving | % DV |
---|---|---|
Calories | 5 | |
Total carbohydrate | 1.0 g | <1 |
Dietary fiber | 0.2 g | 4* |
Vitamin C | 173 mg | 193 |
Thiamin | 0.56 mg | 46 |
Riboflavin | 0.78 mg | 60 |
Niacin | 20 mg | 123 |
Vitamin B6 | 0.98 mg | 58 |
Vitamin B12 | 0.9 mcg | 38 |
Pantothenic acid | 1.7 mg | 34 |
Chromium picolinate | 10 mcg | 3 |
Fat burning matrix | 2003 mg | |
Immunity booster & prebiotic complex | 625 mg | |
Mood enhancer matrix | 851 mg | |
Full B vitamin spectrum | 24.59 mg |
The sufficiency of dietary intake of macro- and micronutrients was evaluated according to reference values of the Turkish Nutrition Guideline.12 Daily copper consumption (DCC) <1.3 mg/day in females and <1.6 mg/day in males was defined as “low DCC group.”12 ,13 (link) The patients and/or their caregivers were given a questionnaire about their attitudes toward dietary copper restriction. Based on their statements, the patients were divided as either “following a copper-restricted diet” or “not restricting copper.” The normal ranges for percentage of energy gained from fat and carbohydrate were defined as 20%-35% and 45%-60%, respectively.12 Minimum required daily fiber intake (g/day) was calculated as “patient age (years) + 5.”