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Pyridoxine

Pyridoxine is a water-soluble vitamin that belongs to the B-complex group.
It is essential for the metabolism of proteins, fats, and carbohydrates, as well as the production of red blood cells and neurotransmitters.
Pyridoxine deficiency can lead to anemia, skin problems, and neurological issues.
Researchers often study pyridoxine to understand its role in human health and develop effective treatments.
PubCompare.ai can help optimize pyridoxine research by allowing easy access to and comparison of relevant protocols from literature, preprints, and patents, enhancing reproducibility and accuracy.
Experieence the power of PubCompare.ai to identify the best methods and products for your pyridoxine research needs.

Most cited protocols related to «Pyridoxine»

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Publication 2018
Folic Acid Homocysteine Placebos Pyridoxine Systole Systolic Pressure
Continuous cultures were established using published methods [54] (link) with the exception of the phosphate-limited media, which contained the following (per liter): 100 mg calcium chloride, 100 mg sodium chloride, 500 mg magnesium sulfate, 5 g ammonium sulfate, 1 g potassium chloride, 500 µg boric acid, 40 µg copper sulfate, 100 µg potassium iodide, 200 µg ferric chloride, 400 µg manganese sulfate, 200 µg sodium molybdate, 400 µg zinc sulfate, 1 µg biotin, 200 µg calcium pantothenate, 1 µg folic acid, 1 mg inositol, 200 µg niacin, 100 µg p-aminobenzoic acid, 200 µg pyridoxine, 100 µg riboflavin, 200 µg thiamine, 10 mg potassium phosphate, and 5 g glucose.
Experiments were started by initially growing cultures in 300mL of the appropriate defined media in batch phase. Once the cultures reached saturation, chemostat flow was initiated. Cultures were grown at a dilution rate of 0.17 volumes/hour. Daily samples were taken from the overflow in order to determine optical density at 600 nm, cell count and viability; perform microscopy; and make archival glycerol stocks. We confirmed that all evolved haploid clones maintained the same mating type as the founder by backcrossing the evolved strain to the isogenic ancestral strain of the opposite mating type. Clones from three of the twelve evolved diploid populations exhibited reduced sporulation efficiency, but did not mate inappropriately.
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Publication 2008
4-Aminobenzoic Acid Biotin boric acid Calcium chloride Clone Cells Diploidy ferric chloride Folic Acid Glucose Glycerin Inositol manganese sulfate Microscopy Niacin Pantothenate, Calcium Phosphates Population Group Potassium Chloride Potassium Iodide potassium phosphate Pyridoxine Riboflavin Sodium Chloride sodium molybdate(VI) Strains Sulfate, Ammonium Sulfate, Copper Sulfate, Magnesium Technique, Dilution Thiamine Vision Zinc Sulfate
The wild-type N. crassa reference strain and background for mutant strains were OR74A (FGSC 2489) (Colot et al. 2006 (link)). The deletion strains for clr-2 (FGSC 15,835) were obtained from the Fungal Genetics Stock Center (http://www.fgsc.net/) (McCluskey 2003 (link)). The clr-2 misexpression strain was constructed by transforming a his-3 rid-1 Δsad-1; Δclr-2 A mutant with a variant of plasmid pMF272, where the open reading frame and 3′ untranslated region (UTR) of clr-2 were placed under the control of the promoter and 5′ UTR of the clock-controlled gene 1 (ccg-1). Transformants were selected for histidine prototrophy and backcrossed to a his-3; Δclr-2 A strain to obtain a his-3::pccg-1-clr-2 rid-1 Δsad-1; Δclr-2 A homokaryotic strain.
The A. nidulans reference strain was FGSC 4A. The ΔclrB deletion strain was constructed previously (Coradetti et al. 2012 (link)). The clrB misexpression strains were constructed by transforming a ΔclrB mutant (ΔclrB::pyrG pyroA4 pyrG89) with a DNA fragment consisting of the Aspergillus fumigatus pyroA gene, the gpdA (Punt et al. 1990 (link)) or the alcA (Waring et al. 1989 (link)) promoter and the coding region, and 3′ UTR of either clrB or clr-2 inserted into the pyrG locus. Transformants were selected for pyridoxine prototrophy (Nayak et al. 2006 (link)). The DNA fragments were generated by fusion PCR and transformation was carried out as described previously (Szewczyk et al. 2006 (link)).
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Publication 2013
3' Untranslated Regions 5' Untranslated Regions Aspergillus fumigatus Deletion Mutation DNA, A-Form Genes Genes, Fungal Histidine PCCG-2 Plasmids Pyridoxine Strains
S. mutans strain UA140 was used as the parent strain. UA140 derivatives constructed in this study are listed in Table S1 of Supplemental Materials. All S. mutans strains were grown in Brain Heart Infusion (BHI, Difco; Sparks, MD) agar plates supplemented with either spectinomycin (Spc; 800 μg/ml), kanamycin (Kan, 800 μg/ml), or erythromycin (Erm; 15 μg/ml). Todd-Hewitt broth (TH) was used in the phenotypic microarray (PM) assay and the EGTA assay for calcium. For luciferase reporter assays, all strains were grown in artificial saliva solution (ASS) with or without added calcium. The ASS medium was modified from the chemically defined medium by Socransky (Socransky et al., 1985 (link)), and contains the following ingredients:
Glu (2.54 g/L), Cys (0.2 g/L), Leu (0.15 g/L), Lys (0.15 g/L), Arg (0.21 g/L), Pro (0.006 g/L), Gly (0.004 g/L), adenine (0.009 g/L), K2HPO4 (11.9 mM), KH2PO4 (21.5 mM), NH4Cl (10 mM), Urea (5 mM), Sodium Pyruvate (5.5 mM), MgCl2 (5.9 mM), MnCl2 (0.1 mM), FeSO4 (0.1 mM), CaCl2 (1 mM), Riboflavin (1 mg/L), Thiamine HCl (0.5 mg/L), D-Biotin (0.1 mg/L), Nicotinic acid (1 mg/L), p-Amino-benzoic acid (0.1 mg/L), a-panthothenate (0.5 mg/L), Pyridoxine (1 mg/L), a-panthothenate (0.5 mg/L), Pyridoxine (1 mg/L), Folic acid (0.1 mg/L), and glucose (0.5 %). The amino acids, salts, and vitamins were made in stock mixtures separately, and filter-sterilized. Glucose was made as a stock, filter-sterilized, and added with other ingredient mixtures. Stock solutions were stored in 4° C and used within one month, and ASS solution was used within one week. All S. mutans strains were grown anaerobically (90% N2 / 5% CO2 / 5% H2) at 37° C.
Publication 2008
Adenine Agar Amino Acids Benzoic Acid Biological Assay Biotin Brain Calcium, Dietary derivatives Egtazic Acid Erythromycin Folic Acid Glucose Heart Kanamycin Luciferases Magnesium Chloride manganese chloride Microarray Analysis Nicotinic Acids Parent Phenotype potassium phosphate, dibasic Pyridoxine Pyruvate Riboflavin Saliva, Artificial Salts Sodium Spectinomycin Strains thiamine hydrochloride Urea Vitamins
In the present study, the workflow for the experiment is illustrated in Fig. 1, and all chickens were obtained from the fast-growing white-feathered pure line B. Line B is a synthetic line produced by Foshan Gaoming Xinguang Agricultural and Animal Industrials Co., Ltd. (Foshan, China), and it has been selected for high body weight and growth rate traits for seven generations. In generation 6, a total of 189 male broiler breeders at 24 d of age, which were produced in the same hatch from 68 sires and 127 dams in generation 5, were randomly selected. They were housed in identical individual cages and provided with water and feed ad libitum. Each day, the amount of fresh feed provided was recorded individually, and residual feed was recorded daily and removed for an intervening period at 28 d of age. The broilers were fed a common corn-soybean meal diet until the end of the trial (42 d of age). The diet contained 2,900 kcal/kg metabolic energy and 183 g/kg crude protein, and detailed information about the diet is summarized in Table 1. The birds were slaughtered at 43 d of age after a 12-h overnight fast to obtain records of carcass traits, including abdominal fat weight (AbF).

The workflow for the experiment in the current study

Ingredient and nutrient composition of the experimental diet

Ingredients, %Nutrients composition
Corn67.35Metabolizable energy, kcal/kg2,900
Soybean meal28.00Crude protein, %18.30
Soybean oil0.40Arginine, %1.14
Limestone1.90Lysine, %1.07
Monocalcium phosphate1.00Methionine, %0.47
Salt0.40Methionine + Cystine, %0.77
Lysine0.22Threonine, %0.73
Methionine0.17Tryptophan, %0.24
Choline chloride0.14Calcium, %1.01
Premix10.42Available phosphorus, %0.31
Total100

1Premix supplied per kilogram of diet: vitamin A, 13,200 IU; vitamin D3, 5,000 IU; vitamin E, 60 IU; vitamin K3, 8.0 mg; thiamine, 4.0 mg; riboflavin, 12.0 mg; pyridoxine, 12.0 mg; cobalamin, 0.4 mg; nicotinic acid, 80 mg; pantothenic acid, 24 mg; folic acid, 2.0 mg; biotin, 3.0 mg; iron, 70.0 mg; copper, 8.5 mg; zinc, 45.0 mg; manganese, 60.0 mg; iodine, 0.85 mg; selenium, 0.20 mg; cobalt, 0.25 mg

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Publication 2020
Abdominal Fat Animals Aves Biotin Chickens Cholecalciferol Cobalt Copper Corn Flour Corns Cystine Diet Folic Acid GTP-Binding Proteins Iodine Iron Males Manganese Niacin Nutrients Pantothenic Acid Phosphorus Proteins Pyridoxine Riboflavin Selenium Soybean Flour Soybeans Thiamine Vitamin A Vitamin B12 Vitamin E Vitamin K3 Zinc

Most recents protocols related to «Pyridoxine»

Example 3

Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 μm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.

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Patent 2024
Clostridium histolyticum Fermentation ferrous sulfate Filtration Niacin Pantothenate, Calcium Pimelic Acid Pyridoxine Riboflavin Sterilization Strains Sulfate, Magnesium Thiamine Vitamins

Example 3

Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 pm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.

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Patent 2024
Clostridium histolyticum Fermentation ferrous sulfate Filtration Niacin Pantothenate, Calcium Pimelic Acid Pyridoxine Riboflavin Sterilization Strains Sulfate, Magnesium Thiamine Vitamins
Hy-Line Brown laying hens were fed with a regular diet (corn-soybean meal-based; containing 0.32% non-phytate phosphorus (NPP); Table 1) start from 35 weeks of age. On the last day of age 40 weeks, a total of 60 hens that laid eggs between 07:30−08:30 were randomly selected to evaluate the daily phosphorus rhythms. Of them, 45 hens were euthanized for sample collection, and the other 15 hens were used to study the feed intake and calcium/phosphorus excretion rhythms. For sample collection, the 45 hens were sampled according the oviposition cycle: at oviposition, at 6, 12, 18 h post-oviposition, and at the next oviposition, respectively, with 9 hens sampled at each of the time point. The following samples were collected: blood (for serum), uterine (stored at −80 ℃, for Western-blotting analysis), femur (in 4% paraformaldehyde, for histological analysis) and kidney (stored at −80 ℃, for Western-blotting analysis). For the other 15 hens, the feed intake was recoded and the excreta was collected at the following intervals: from oviposition to 6 h post-oviposition, from 7 to 12 h post-oviposition, from 13 to 18 h post-oviposition, from 19 h post-oviposition to the next oviposition.

Composition and nutrient concentrations of basal diet (%, unless noted, as-is basis)

ItemLow phosphorusRegular phosphorus
Ingredients
 Corn56.6956.69
 Soybean meal25.7725.77
 Distillers dried grains with solubles4.004.00
 Calcium carbonate9.739.04
 Dicalcium phosphate-1.15
 Soybean oil1.511.51
 Sodium chloride0.260.26
DL-Methionine0.180.18
 Choline chloride0.150.15
 Montmorillonite0.710.25
 Premix111
 In total100.00100.00
Nutrient levels
 Metabolizable energy, kcal/kg (calculated)2,6002,600
 Crude protein (calculated)16.516.5
 Total phosphorus (calculated/analyzed)0.34/0.340.53/0.49
 Non-phytate phosphorus (calculated)0.140.32
 Calcium (calculated/analyzed)3.50/3.473.50/3.52

1Provided per kilogram of diet: manganese 60 mg, copper 8 mg, zinc 80 mg, iodine 0.35 mg, selenium 0.3 mg, vitamin A 8000 IU, vitamin E 30 mg, vitamin K3 1.5 mg, thiamine 4 mg, riboflavin 13 mg, pantothenic acid 15 mg, nicotinamide 20 mg, pyridoxine 6 mg, biotin 0.15 mg, folic acid 1.5 mg, and cobalamin 0.02 mg

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Publication 2023
Biotin BLOOD Calcium, Dietary calcium phosphate Cereals Choline Copper Corn Flour Corns Diet Eggs Feed Intake Femur Folic Acid Iodine Kidney Manganese Niacinamide Nutrients Oviposition Pantothenic Acid paraform Phosphorus Phytate Proteins Pyridoxine Riboflavin Selenium Serum Sodium sodium phosphate Soybean Flour Soybeans Specimen Collection Thiamine Uterus Vitamin A Vitamin B12 Vitamin E Vitamin K3 Western Blot Zinc-80
A cofactor-protein interaction network for vitamin B2 (riboflavin), B5 (pantothenate), and B6 (pyridoxine) and its interacting proteins were generated using pre-published data in cytoscape 3.8.2113 (link),114 (link). A network was generated for each transcriptomic dataset. A comparative analysis was carried out for common genes between the cofactor-protein network and those involved in the enriched pathways from transcriptomic dataset analysis. Further, Cytoscape 3.8.2 was used to generate the integrated Transcriptomic-Cofactor-protein interaction network using the network merge tool for visualization. The generated network highlights key overlapping genes that are represented in both the cofactor-protein network and enriched pathway network from transcriptomics as shown.
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Publication 2023
Gene Expression Profiling Genes Genes, Overlapping Proteins Pyridoxine Riboflavin Vitamins
Real-time OD600 measurements were obtained every 10 min for 48–120 h with microplate reader Synergy™ H1 (BioTek) for aerobic and microaerobic conditions. Epoch 2 microplate reader (BioTek) was used for anaerobic real-timer OD600 measurements. All cultures had an initial OD600 of 0.05. The cultures were incubated into 200 µL minimal synthetic complete media (DELFT) containing 7.5 g/L (NH4)2SO4, 14.4 g/L KH2PO4, 0.5 g/L MgSO4 x 7H2O, 20 g/L glucose, 2 ml/L trace metals solution, and 1 ml/L vitamins with or without thiamine and pyridoxine (Verduyn et al., 1992 (link)), and with or without 20 mg/L uracil, 20 mg/L histidine and 20 mg/L tryptophan supplemented. CELLSTAR® 96 well cell culture plate (Greiner Bio-One) with an air-penetrable lid (Breathe-Easy, Diversified Biotech) was used for all cultivation. pH was adjusted with 1M HCl to 3, 4, 5 and 6 for the respective experiment. Cultivation was performed with continuous double orbital shaking of 548 cycles per minute (CPM) at 37 °C and 0%, 0.1%, 1% or 21% oxygen. Anaerobic conditions were obtained using a vinyl anaerobic chamber (Coy Laboratory Products Vinyl; gas mixture, 95% N2 and 5% H2), and microaerobic conditions were obtained using CO2/O2 Gas Controller (BioTek).
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Publication 2023
Bacteria, Aerobic Cell Culture Techniques EPOCH protocol Glucose Histidine Metals Polyvinyl Chloride Pyridoxine Sulfate, Magnesium Thiamine Tryptophan Uracil Vitamins

Top products related to «Pyridoxine»

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Pyridoxine is a chemical compound used in laboratory settings. It is a form of vitamin B6 and plays a role in various metabolic processes. Pyridoxine is used as a standard for analytical and research purposes.
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Riboflavin, also known as vitamin B2, is a water-soluble vitamin that is commonly used in laboratory settings. It serves as a core component in various biological processes, including energy metabolism and cellular respiration. Riboflavin plays a crucial role as a cofactor for enzymes involved in the conversion of food into energy. This product is often used in research and analytical applications where its specific properties and functions are required.
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Nicotinic acid, also known as niacin, is a water-soluble vitamin that plays a vital role in various metabolic processes within the human body. It serves as a precursor to the important coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), which are essential for cellular energy production, DNA repair, and other biochemical reactions.
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Formic acid is a clear, colorless liquid chemical compound used in various industrial and laboratory applications. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid has a pungent odor and is highly corrosive. It is commonly used as a preservative, pH adjuster, and analytical reagent in laboratory settings.
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HEPES is a buffering agent commonly used in cell culture and biochemical applications. It helps maintain a stable pH environment for biological processes.
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Pyridoxine hydrochloride is a chemical compound that is commonly used as a form of vitamin B6. It is a white, crystalline solid with the chemical formula C₈H₁₁NO₃•HCl. Pyridoxine hydrochloride is an essential nutrient that plays a role in various metabolic processes within the body.
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Formic acid is a colorless, pungent-smelling liquid chemical compound. It is the simplest carboxylic acid, with the chemical formula HCOOH. Formic acid is widely used in various industrial and laboratory applications.
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Acetonitrile is a highly polar, aprotic organic solvent commonly used in analytical and synthetic chemistry applications. It has a low boiling point and is miscible with water and many organic solvents. Acetonitrile is a versatile solvent that can be utilized in various laboratory procedures, such as HPLC, GC, and extraction processes.
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Folic acid is a laboratory reagent used in various scientific applications. It is a water-soluble vitamin that plays a crucial role in cellular function and development. Folic acid is an essential component in many biochemical processes, including DNA synthesis and cell growth.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

More about "Pyridoxine"

Pyridoxine, also known as vitamin B6, is a water-soluble nutrient that plays a crucial role in human health.
Part of the B-complex group, pyridoxine is essential for the metabolism of proteins, fats, and carbohydrates, as well as the production of red blood cells and neurotransmitters.
Deficiency in pyridoxine can lead to a range of issues, including anemia, skin problems, and neurological complications.
Researchers frequently study pyridoxine to understand its impact on human wellbeing and develop effective treatments.
Riboflavin, another B-vitamin, is often examined alongside pyridoxine, as they work in tandem to support various bodily functions.
Nicotinic acid, also known as niacin, is another B-vitamin that is closely related to pyridoxine and plays a role in energy production and metabolism.
Formic acid, HEPES, and acetonitrile are common chemical compounds used in the analysis and extraction of pyridoxine.
Pyridoxine hydrochloride is a specific form of the vitamin that is often used in supplements and research.
Fetal bovine serum (FBS) is a widely used cell culture supplement that may contain pyridoxine and other essential nutrients.
PubCompare.ai is a powerful tool that can help optimize pyridoxine research by providing easy access to and comparison of relevant protocols from literature, preprints, and patents.
This enhances the reproducibility and accuracy of pyridoxine-related studies, allowing researchers to identify the best methods and products for their specific needs.
Experieence the power of PubCompare.ai to take your pyridoxine research to the next level.