FFQ-derived dietary information was used to calculate DII scores for all of the subjects, as described in detail elsewhere(24 (link),25 (link)). Briefly, dietary data for each study participant were first linked to a regionally representative global database that provided a robust estimate of means and standard deviations for each of the food parameters considered (i.e. foods, nutrients and other food components such as flavonoids)(24 (link)). A z-score was derived by subtracting the ‘standard global mean’ from the amount reported, and then this value was divided by the standard deviation. To minimise the effect of ‘right skewing’ (a common occurrence with dietary data), this value was then converted to a centred percentile score, which was then multiplied by the respective inflammatory effect score of the food parameters (derived from a literature review and scoring of 1943 ‘qualified’ articles) to obtain the subject’s food parameter-specific DII score. All of the food parameter-specific DII scores were then summed to create the overall DII score for each subject in the study. For the current FFQ, data were available for a total of seventeen food parameters (carbohydrate, protein, total fat, fibre, cholesterol, saturated fat, monounsaturated fat, polyunsaturated fat, n-6 fatty acid, thiamin, riboflavin, vitamin B12, Fe, Mg, Zn, vitamin A and vitamin C). A description of the validation work of the DII score, based on both dietary recalls and a structured questionnaire, the 7 d dietary recall that is similar to an FFQ, is available elsewhere(26 (link)). Thus far, the DII has been found to be associated with inflammatory cytokines, including CRP and IL-6(26 (link),31 (link),32 (link)), the glucose intolerance component of the metabolic syndrome, the increased odds of asthma and FEV1 (reduced forced expiratory volume in 1 min), inflammatory markers in shift workers, and colorectal, prostate and pancreatic cancers(31 (link)–38 ).
Thiamine
It plays a crucial role in energy production, nerve function, and carbohydrate metabolism.
Thiamine deficiency can lead to serious health issues, such as beriberi and Wernicke-Korsakoff syndrome.
Researchers use PubCompare.ai to optimize thiamine research by empowering users to locate the best protocols and products.
The AI-driven comparisons enhance reproducibility and accuracy, helping researchers make informed decisions.
Explore the power of data-driven protocol comparison to eleviate your thiamie studies and advance the understanding of this vital nutrient.
Most cited protocols related to «Thiamine»
We selected 24 nutrients for inclusion in the PANDiet: protein, total carbohydrate, fibre, total fat, saturated and polyunsaturated fatty acids, cholesterol, thiamin, riboflavin, niacin, folate, vitamins A, B-6, B-12, C, D and E, calcium, magnesium, zinc, phosphorus, potassium, iron and sodium. This selection was based on the available current national nutritional recommendations for French [25] –[30] and US adults [31] –[38] , and the availability of data in ENNS and NHANES food composition databases.
We used the probabilistic approach developed by the Institute of Medicine [20] to estimate, for each individual, if the usual intake of a nutrient was adequate. The calculation of the probability takes into account the number of days of dietary data, the mean intake and the day-to-day variability of intake, the nutrient reference value and the interindividual variability (
For each nutrient, adequate intake was assumed to be the level likely to satisfy the nutrient requirements and unlikely to be excessive and elicit adverse health effects. Therefore, we assessed separately the probability that the intake was adequate inasmuch as it satisfied the requirement, on one hand, and the probability that it was not excessive, on the other hand. Consequently, the PANDiet was constructed based on two sub-scores - the Adequacy sub-score and the Moderation sub-score.
The Adequacy sub-score was calculated as the average of the probability of adequacy for items for which the usual intake should be above a reference value, multiplied by 100. According to the nutrient reference values, the probability was determined as follows:
The Moderation sub-score was calculated as the average of the probability of adequacy for items for which the usual intake should not exceed a reference value and penalty values, multiplied by 100. According to the nutrient reference values, the probability was determined as follows:
For other vitamins and minerals with available upper tolerable limits but where the risk of excessive intake is low, we used a penalty value system: a value equal to 0 was generated when the average intake of a nutrient exceeded the upper tolerable limit of intake.
The PANDiet score is the average of the Adequacy and Moderation sub-scores. In principle, the score ranges from 0 to 100; the higher the score, the better the diet quality.
A French implementation of the PANDiet (
A canonic rDNA unit was assembled. A junction sequence between the left arm of chromosome 3 and an rDNA unit was observed, confirming the position of the cluster on this chromosome based on pulse field electrophoresis data [28 (link)]. On the other end of the cluster a junction between an incomplete rDNA repeat and CCCTAA telomeric repeats [88 (link)] was detected showing that the cluster is in a subtelomeric position. Similar to the previously investigated filamentous fungi [89 (link)], 5S rRNAs were detected by comparison with the N. crassa 5S genes. They are encoded by a set of 87 genes, including 72 full-length copies dispersed in the genome. tRNAs were identified with tRNAscan [90 (link)]. A total of 361 genes encode the cytosolic tRNA set, which is composed of 48 different acceptor families containing up to 22 members. This set enabled us to decode the 61 sense codons with the classical wobble rule. Other non-coding RNAs were detected with a combination of the Erpin [91 (link)], Blast [92 (link)] and Yass [93 (link)] programs. Homology search included all RNAs contained in the RFAM V.8 [94 (link)] and ncRNAdb [95 (link)] databases. Any hit from either program with an e-value below 10-4 was retained, producing a list of 28 annotated non-coding RNA genes or elements, including 12 spliceosomal RNAs, 15 snoRNAs (mostly of the C/D box class) and one thiamine pyrophosphateriboswitch.
Escherichia coli strain DH5α was used to propagate all plasmids. The Aspergillus species used for implementation of CRISPR-Cas9 are listed in
Most recents protocols related to «Thiamine»
Example 2
100 mg of the Sarcodon aspratus extracts according to the present invention;
an appropriate amount of a vitamin mixture;
70 μg of vitamin A acetate;
1.0 mg of vitamin E;
0.13 mg of vitamin B1;
0.15 mg of vitamin B2;
0.5 mg of vitamin B6;
0.2 μg of vitamin B12;
10 mg of vitamin C;
10 μg of biotin;
1.7 mg of nicotinic acid amide;
50 μg of folate;
0.5 mg of calcium pantothenate;
an appropriate amount of a mineral mixture;
1.75 mg of ferrous sulfide;
0.82 mg of zinc oxide;
25.3 mg of magnesium carbonate;
15 mg of potassium phosphate monobasic;
55 mg of dicalcium phosphate;
90 mg of potassium citrate;
100 mg of calcium carbonate; and
24.8 mg of magnesium chloride.
The composition ratio of the vitamins and the mineral mixture described above may be determined according to a composition ratio used in general functional health foods, and the combination ratio of the vitamins and the mineral mixture may be arbitrarily determined. According to a conventional method of preparing functional health foods, these components are mixed, granules are prepared, and the granules are used to prepare a composition for a functional health food.
EXAMPLE 2
This example shows the composition of the sealant plug covering the hole in the pipe and the pipe remains in the field of repair of.
Example 6
This example provides a representative example of an aqueous solution to be used in a method described herein. The composition may contain the following ingredients:
The aqueous solution is formulated in physiological saline and adjusted to about pH 7.4, thereby minimizing any injecting pain beyond the needle prick. In addition, the aqueous solution may be optionally supplemented with a preservative (e.g., benzyl alcohol), a chemical stabilizer (e.g., gentisic acid), and/or an additional bioactive agent (e.g., platelet-rich plasma) depending on anticipated delivery method, shelf-life, and intended effects.
Example 7
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) twice a day for a period of 29 days. At the end of the 29 day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
-
- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
Example 8
The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.
Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) on days 1, 3, 7, 10, 14, 21, 30, 60, and 90 of treatment. At the end of the 90-day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:
-
- (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
- (2) effectiveness of the composition described herein in improving the overall appearance of skin;
- (3) effectiveness of the composition described herein in evening out skin tone and texture;
- (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
- (5) effectiveness of the composition described herein in making the skin look younger; and
- (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
- (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.
Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.
While preferred embodiments have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the embodiments. It should be understood that various alternatives to the embodiments described herein may be employed. It is intended that the following claims define the scope of the embodiments and that methods and structures within the scope of these claims and their equivalents be covered thereby.
Example 5
An aqueous solution containing the following ingredients:
-
- about 1500 to about 6250 meg cobalamin (vitamin B12);
- about 150 to about 250 mg ascorbic acid (vitamin C);
- about 30 to about 50 mg nicotinamide (vitamin B3);
- about 4.5 to about 7.5 mg thiamine (vitamin B1);
- about 0.1 to about 0.3 mg pyridoxine HCl (vitamin B6);
- about 2.7 to about 4.5 mg riboflavin 5-phosphate sodium (vitamin B2);
- about 7.5 to about 15 mg pantothenic acid (vitamin B5);
- about 0.08 to about 0.125 mg Zinc sulfate heptahydrate; and
- about 1 ml q.s., sterile water for injection
The aqueous solution is formulated in physiological saline and adjusted an acceptable pH in the range of about 6.5 to about 7.5 to 7.4, thereby minimizing any injecting pain beyond the needle prick. The aqueous solution may be optionally supplemented with a preservative (e.g., from about 0.01% to about 2% benzyl alcohol), a chemical stabilizer (e.g., from about 0.01% to about 2% gentisic acid), and/or an additional bioactive agent (e.g., from about 0.01% to about 2% hyaluronic acid) depending on anticipated delivery method, shelf-life, and intended effects.
Example 3
Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 μm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.
Top products related to «Thiamine»
More about "Thiamine"
This water-soluble vitamin is vital for energy production, nerve function, and carbohydrate metabolism.
Deficiency in thiamine can lead to serious health issues, such as beriberi and Wernicke-Korsakoff syndrome.
Researchers utilize advanced tools like PubCompare.ai to optimize their thiamine studies.
This AI-driven platform empowers users to locate the best protocols and products, enhancing the reproducibility and accuracy of their research.
By comparing data-driven insights, researchers can make informed decisions that eleviate their thiamine investigations and advance the understanding of this vital micronutrient.
Beyond thiamine, other B-complex vitamins like riboflavin (vitamin B2) and biotin (vitamin B7) also play important roles in metabolism and overall health.
The combination of these essential nutrients, along with compounds like L-DOPA (a precursor to dopamine), can have synergistic effects on the body's physiological processes.
In the lab, thiamine research may involve the use of thiamine hydrochloride, a common form of the vitamin, as well as other compounds like chloramphenicol, glucose, and select agar media.
The careful selection and optimization of these materials and protocols, facilitated by tools like PubCompare.ai, can help researchers achieve more reliable and meaningful results.
OtherTerms: vitamin B1, energy production, nerve function, carbohydrate metabolism, beriberi, Wernicke-Korsakoff syndrome, PubCompare.ai, riboflavin, biotin, L-DOPA, thiamine hydrochloride, chloramphenicol, glucose, select agar, KH2PO4, ampicillin