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Thiamine

Thiamine, also known as vitamin B1, is an essential nutrient required for proper metabolic functioning.
It plays a crucial role in energy production, nerve function, and carbohydrate metabolism.
Thiamine deficiency can lead to serious health issues, such as beriberi and Wernicke-Korsakoff syndrome.
Researchers use PubCompare.ai to optimize thiamine research by empowering users to locate the best protocols and products.
The AI-driven comparisons enhance reproducibility and accuracy, helping researchers make informed decisions.
Explore the power of data-driven protocol comparison to eleviate your thiamie studies and advance the understanding of this vital nutrient.

Most cited protocols related to «Thiamine»

Participants were asked to complete a semi-quantitative FFQ that included questions on their habitual daily consumption of twenty-five food items during the past year(28 (link)). This FFQ was based on an existing FFQ used in this population and on a short FFQ (i.e. sixty items) developed by Willett(29 (link),30 ). Participants were asked to indicate how often they consumed each item in a list of frequencies (every day; 5–6 d/week; 2–4 d/week; 1 d/week; 1–3 times/month; never or less than once a month), and to indicate approximate portion size.
FFQ-derived dietary information was used to calculate DII scores for all of the subjects, as described in detail elsewhere(24 (link),25 (link)). Briefly, dietary data for each study participant were first linked to a regionally representative global database that provided a robust estimate of means and standard deviations for each of the food parameters considered (i.e. foods, nutrients and other food components such as flavonoids)(24 (link)). A z-score was derived by subtracting the ‘standard global mean’ from the amount reported, and then this value was divided by the standard deviation. To minimise the effect of ‘right skewing’ (a common occurrence with dietary data), this value was then converted to a centred percentile score, which was then multiplied by the respective inflammatory effect score of the food parameters (derived from a literature review and scoring of 1943 ‘qualified’ articles) to obtain the subject’s food parameter-specific DII score. All of the food parameter-specific DII scores were then summed to create the overall DII score for each subject in the study. For the current FFQ, data were available for a total of seventeen food parameters (carbohydrate, protein, total fat, fibre, cholesterol, saturated fat, monounsaturated fat, polyunsaturated fat, n-6 fatty acid, thiamin, riboflavin, vitamin B12, Fe, Mg, Zn, vitamin A and vitamin C). A description of the validation work of the DII score, based on both dietary recalls and a structured questionnaire, the 7 d dietary recall that is similar to an FFQ, is available elsewhere(26 (link)). Thus far, the DII has been found to be associated with inflammatory cytokines, including CRP and IL-6(26 (link),31 (link),32 (link)), the glucose intolerance component of the metabolic syndrome, the increased odds of asthma and FEV1 (reduced forced expiratory volume in 1 min), inflammatory markers in shift workers, and colorectal, prostate and pancreatic cancers(31 (link)–38 ).
Publication 2015
Acids, Omega-6 Fatty Ascorbic Acid Asthma Carbohydrates Cholesterol Cobalamins Cytokine Diet Fibrosis Flavonoids Food Inflammation Intolerances, Glucose Mental Recall Nutrients Pancreatic Cancer Prostate Proteins Riboflavin Saturated Fatty Acid Thiamine Vitamin A Volumes, Forced Expiratory Workers
The PANDiet aims to measure the overall diet quality of an individual through the probability of having an adequate nutrient intake.
We selected 24 nutrients for inclusion in the PANDiet: protein, total carbohydrate, fibre, total fat, saturated and polyunsaturated fatty acids, cholesterol, thiamin, riboflavin, niacin, folate, vitamins A, B-6, B-12, C, D and E, calcium, magnesium, zinc, phosphorus, potassium, iron and sodium. This selection was based on the available current national nutritional recommendations for French [25] –[30] and US adults [31] –[38] , and the availability of data in ENNS and NHANES food composition databases.
We used the probabilistic approach developed by the Institute of Medicine [20] to estimate, for each individual, if the usual intake of a nutrient was adequate. The calculation of the probability takes into account the number of days of dietary data, the mean intake and the day-to-day variability of intake, the nutrient reference value and the interindividual variability (Figure 1). Values range from 0 to 1, where 1 represents a 100% probability that the usual intake was adequate
For each nutrient, adequate intake was assumed to be the level likely to satisfy the nutrient requirements and unlikely to be excessive and elicit adverse health effects. Therefore, we assessed separately the probability that the intake was adequate inasmuch as it satisfied the requirement, on one hand, and the probability that it was not excessive, on the other hand. Consequently, the PANDiet was constructed based on two sub-scores - the Adequacy sub-score and the Moderation sub-score.
The Adequacy sub-score was calculated as the average of the probability of adequacy for items for which the usual intake should be above a reference value, multiplied by 100. According to the nutrient reference values, the probability was determined as follows:
The Moderation sub-score was calculated as the average of the probability of adequacy for items for which the usual intake should not exceed a reference value and penalty values, multiplied by 100. According to the nutrient reference values, the probability was determined as follows:
For other vitamins and minerals with available upper tolerable limits but where the risk of excessive intake is low, we used a penalty value system: a value equal to 0 was generated when the average intake of a nutrient exceeded the upper tolerable limit of intake.
The PANDiet score is the average of the Adequacy and Moderation sub-scores. In principle, the score ranges from 0 to 100; the higher the score, the better the diet quality.
A French implementation of the PANDiet (Figure 2) was developed based on the French nutritional recommendations for adults [25] –[27] , including European Community values when specific French recommendations did not exist [28] –[30] . A US implementation of the PANDiet (Figure 3) was developed based on the US nutritional recommendations for adults [31] –[38] . Although the structure of these two implementations is almost identical, it should be noted that the differences in reference values renders cross-national comparisons of PANDiet scores meaningless.
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Publication 2012
Adult Calcium, Dietary Carbohydrates Cholesterol Diet Fibrosis Folate Food Iron Magnesium Minerals Niacin Nutrient Intake Nutrients Nutritional Requirements Phosphorus Polyunsaturated Fatty Acids Potassium Proteins Riboflavin Sodium Thiamine Vitamins Zinc
C. crescentus cells were grown in M2G medium supplemented with 1% PYE in the presence of appropriate antibiotics (kanamycin, gentamycin). Log-phase cultures were used for all experiments. Expression from Pxyl and Pvan was induced by adding to the medium 0.3% xylose and 500 μM vanillic acid, respectively. The synchrony was performed as described (Evinger & Agabian, 1977 (link)). E. coli cells were grown in M9 minimal medium supplemented with 1 mM MgSO4, 0.2% casamino acids, 50 μM thiamine, 0.2% of either maltose or glycerol in the presence of appropriate antibiotics (ampicillin, chloramphenicol). Strains and plasmids are listed in Table S1.
Publication 2011
Ampicillin Antibiotics, Antitubercular casamino acids Cells Chloramphenicol Escherichia coli Gentamicin Glycerin Kanamycin Maltose Plasmids Strains Sulfate, Magnesium Thiamine Vanillic Acid Xylose
CDSs were annotated by a combination of semi-automatic procedures. First, P. anserina open reading frames longer than 20 codons that are evolutionary conserved in N. crassa were retrieved by TBLASTN analysis. Candidates with an e-value lower than 10-18 were conserved as hypothetical exons. Exons separated by less than 200 nucleotides were merged into putative CDSs and putative introns were predicted thanks to the P. anserina consensus sequences defined in the pilot project [24 (link)]. Then, 5' and 3' smaller exons were searched by the same procedure except that open reading frames longer than five codons surrounding putative CDSs were analyzed by BLAST with the homologous N. crassa region. Candidates with an e-value lower than 10-5 were conserved and added to the putative CDSs. CDS and intron predictions were edited with Artemis [86 (link)] and manually corrected after comparison with available ESTs. Finally, ab initio prediction with GeneID [87 (link)] using the N. crassa and Chaetomium globosum parameter files were performed on regions devoid of annotated features. Manual verification was then applied to improve prediction. This resulted in the definition of 10,545 putative CDSs.
A canonic rDNA unit was assembled. A junction sequence between the left arm of chromosome 3 and an rDNA unit was observed, confirming the position of the cluster on this chromosome based on pulse field electrophoresis data [28 (link)]. On the other end of the cluster a junction between an incomplete rDNA repeat and CCCTAA telomeric repeats [88 (link)] was detected showing that the cluster is in a subtelomeric position. Similar to the previously investigated filamentous fungi [89 (link)], 5S rRNAs were detected by comparison with the N. crassa 5S genes. They are encoded by a set of 87 genes, including 72 full-length copies dispersed in the genome. tRNAs were identified with tRNAscan [90 (link)]. A total of 361 genes encode the cytosolic tRNA set, which is composed of 48 different acceptor families containing up to 22 members. This set enabled us to decode the 61 sense codons with the classical wobble rule. Other non-coding RNAs were detected with a combination of the Erpin [91 (link)], Blast [92 (link)] and Yass [93 (link)] programs. Homology search included all RNAs contained in the RFAM V.8 [94 (link)] and ncRNAdb [95 (link)] databases. Any hit from either program with an e-value below 10-4 was retained, producing a list of 28 annotated non-coding RNA genes or elements, including 12 spliceosomal RNAs, 15 snoRNAs (mostly of the C/D box class) and one thiamine pyrophosphateriboswitch.
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Publication 2008
Biological Evolution Chaetomium globosum Chromosomes Chromosomes, Human, Pair 3 Codon Consensus Sequence Cytosol DNA, Ribosomal Electrophoresis Exons Expressed Sequence Tags Fungus, Filamentous Genes Genes, vif Genome Introns Nucleotides Open Reading Frames Pulse Rate RNA RNA, Ribosomal, 5S RNA, Untranslated Sense Codon Small Nucleolar RNA Spliceosomes Telomere Thiamine Transfer RNA Triglyceride Storage Disease with Ichthyosis

Escherichia coli strain DH5α was used to propagate all plasmids. The Aspergillus species used for implementation of CRISPR-Cas9 are listed in Table 2. Genomic DNA (gDNA) from fungal strains was isolated via FastDNA SPIN Kit for Soil DNA extraction kit (MP Biomedicals, USA). The mutant strains made in this study are listed in Table 1. All aspergilli were cultivated on standard solid glucose based minimal medium (MM) (1% glucose, 1x nitrate salt solution [41 ], 0.001% Thiamine, 1x trace metal solution [42 (link)], 2% agar), supplemented with 10 mM uridine (Uri), 10 mM uracil (Ura), and/or 4 mM L-arginine (Arg) when required. Solid plates containing 5-fluoroorotic acid (5-FOA) were made as MM+Arg+Uri+Ura supplemented with filter-sterilized 5-FOA (Sigma-Aldrich) to a final concentration of 1.3 mg/ml. For transformation media (TM) glucose was replaced with 1 M sucrose.
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Publication 2015
5-fluoroorotic acid Agar Arginine Aspergillus Clustered Regularly Interspaced Short Palindromic Repeats DNA, Fungal Escherichia coli Genome Glucose Metals Nitrates Plasmids Strains Sucrose Thiamine Uracil Uridine

Most recents protocols related to «Thiamine»

Not available on PMC !

Example 2

100 mg of the Sarcodon aspratus extracts according to the present invention;

an appropriate amount of a vitamin mixture;

70 μg of vitamin A acetate;

1.0 mg of vitamin E;

0.13 mg of vitamin B1;

0.15 mg of vitamin B2;

0.5 mg of vitamin B6;

0.2 μg of vitamin B12;

10 mg of vitamin C;

10 μg of biotin;

1.7 mg of nicotinic acid amide;

50 μg of folate;

0.5 mg of calcium pantothenate;

an appropriate amount of a mineral mixture;

1.75 mg of ferrous sulfide;

0.82 mg of zinc oxide;

25.3 mg of magnesium carbonate;

15 mg of potassium phosphate monobasic;

55 mg of dicalcium phosphate;

90 mg of potassium citrate;

100 mg of calcium carbonate; and

24.8 mg of magnesium chloride.

The composition ratio of the vitamins and the mineral mixture described above may be determined according to a composition ratio used in general functional health foods, and the combination ratio of the vitamins and the mineral mixture may be arbitrarily determined. According to a conventional method of preparing functional health foods, these components are mixed, granules are prepared, and the granules are used to prepare a composition for a functional health food.

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Patent 2024
Ascorbic Acid Biotin Carbonate, Calcium Cobalamins Cytoplasmic Granules dicalcium phosphate ferrous sulfide Folate Functional Food magnesium carbonate Magnesium Chloride magnesium citrate Minerals Niacinamide Pantothenate, Calcium Potassium Potassium Citrate potassium phosphate retinol acetate Riboflavin Sarcodon aspratus Thiamine Vitamin A Vitamin B6 Vitamin E Vitamins Zinc Oxide
Not available on PMC !

EXAMPLE 2

This example shows the composition of the sealant plug covering the hole in the pipe and the pipe remains in the field of repair of.

CAS #Name component materialContent, %
80-05-7Bisphenol A39
668609-97-2Glycidyl ether11
21645-51-2Inert powder filler (synthetic aluminum20
67-53-0A corrosion resistance provider3
120962-03Rapeseed oil6.1
112945-52Silicon dioxide1
20344-49Iron oxide hydroxyl0.3
1140-40-0Diethylene thiamine3.5
80-05-74.4Isopropylidenediphenol2.5
2855-13-2Isophorondiamine4
100-51-6Benzyl alcohol4
9009-54-5Polyurethane2
9003-04-7Polyacrylate (Tamcril-15)3
9004-62-9Berol NP-10 (9)0.4
9004-62-0Hydroxyethyl cellulose0.1
8050-81-5Antifoam APRU DF-70100.1

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Patent 2024
Aluminum Benzyl Alcohol bisphenol A Corrosion Ethanol Ethers ferric oxide hydroxyethylcellulose Hydroxyl Radical Iron NP 10 Oil, Rapeseed Polyurethanes Powder Silicon Silicon Dioxide Thiamine

Example 6

This example provides a representative example of an aqueous solution to be used in a method described herein. The composition may contain the following ingredients:

5000 mcgcobalamin (vitamin B12)
200 mgascorbic acid (vitamin C)
 40 mgnicotinamide (vitamin B3)
 6 mgthiamine (vitamin B1)
 6 mgpyridoxine HCl (vitamin B6)
 3.6 mg riboflavin 5-phosphate sodium (vitamin B2)
 10 mgpantothenic acid (vitamin B5)
 0.1 mg Zinc sulfate heptahydrate
1 mlq.s., sterile water for injection

The aqueous solution is formulated in physiological saline and adjusted to about pH 7.4, thereby minimizing any injecting pain beyond the needle prick. In addition, the aqueous solution may be optionally supplemented with a preservative (e.g., benzyl alcohol), a chemical stabilizer (e.g., gentisic acid), and/or an additional bioactive agent (e.g., platelet-rich plasma) depending on anticipated delivery method, shelf-life, and intended effects.

Example 7

The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.

Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) twice a day for a period of 29 days. At the end of the 29 day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:

    • (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
    • (2) effectiveness of the composition described herein in improving the overall appearance of skin;
    • (3) effectiveness of the composition described herein in evening out skin tone and texture;
    • (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
    • (5) effectiveness of the composition described herein in making the skin look younger; and
    • (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
    • (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.

Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.

Example 8

The purpose of this example is to evaluate the efficacy of a composition described herein in treating the signs of aging present on facial skin. A composition is prepared as in Example 6.

Two groups of human subjects (8-10 subjects per group) are injected with the composition or physiological saline (control) on days 1, 3, 7, 10, 14, 21, 30, 60, and 90 of treatment. At the end of the 90-day test period, the subjects are polled regarding various aspects of the effectiveness of the composition described herein in treating and/or minimizing signs of aging present on the skin. The following aspects of the composition described herein are evaluated:

    • (1) effectiveness of the composition described herein in improving the smoothness and/or softness of skin (i.e., making the skin feel smoother and softer following treatment);
    • (2) effectiveness of the composition described herein in improving the overall appearance of skin;
    • (3) effectiveness of the composition described herein in evening out skin tone and texture;
    • (4) effectiveness of the composition described herein in improving the clarity and/or radiance of skin;
    • (5) effectiveness of the composition described herein in making the skin look younger; and
    • (6) effectiveness of the composition described herein in making wrinkles appear softer and/or less prominent.
    • (7) effectiveness of the composition described herein in increasing the degree of hydration of the skin.

Patients treated with the composition exhibit improvement in one or more of the symptoms described herein.

While preferred embodiments have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the embodiments. It should be understood that various alternatives to the embodiments described herein may be employed. It is intended that the following claims define the scope of the embodiments and that methods and structures within the scope of these claims and their equivalents be covered thereby.

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Patent 2024
Ascorbic Acid Benzyl Alcohol Cobalamins Face Feelings gentisic acid Needles Niacin Niacinamide Obstetric Delivery Pain Pantothenic Acid Patients Pharmaceutical Preservatives physiology Platelet-Rich Plasma Pyridoxine Hydrochloride Riboflavin Saline Solution Skin Skin Pigmentation Sodium Riboflavin Phosphate Sterility, Reproductive Thiamine Vitamin B6 Vitamins Youth Zinc Sulfate, Heptahydrate
Not available on PMC !

Example 5

An aqueous solution containing the following ingredients:

    • about 1500 to about 6250 meg cobalamin (vitamin B12);
    • about 150 to about 250 mg ascorbic acid (vitamin C);
    • about 30 to about 50 mg nicotinamide (vitamin B3);
    • about 4.5 to about 7.5 mg thiamine (vitamin B1);
    • about 0.1 to about 0.3 mg pyridoxine HCl (vitamin B6);
    • about 2.7 to about 4.5 mg riboflavin 5-phosphate sodium (vitamin B2);
    • about 7.5 to about 15 mg pantothenic acid (vitamin B5);
    • about 0.08 to about 0.125 mg Zinc sulfate heptahydrate; and
    • about 1 ml q.s., sterile water for injection

The aqueous solution is formulated in physiological saline and adjusted an acceptable pH in the range of about 6.5 to about 7.5 to 7.4, thereby minimizing any injecting pain beyond the needle prick. The aqueous solution may be optionally supplemented with a preservative (e.g., from about 0.01% to about 2% benzyl alcohol), a chemical stabilizer (e.g., from about 0.01% to about 2% gentisic acid), and/or an additional bioactive agent (e.g., from about 0.01% to about 2% hyaluronic acid) depending on anticipated delivery method, shelf-life, and intended effects.

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Patent 2024
5'-O-(6-O-malonylglucopyranosyl)pyridoxine Ascorbic Acid Benzyl Alcohol Cobalamins gentisic acid Hyaluronic acid Needles Niacin Niacinamide Obstetric Delivery Pain Pantothenic Acid Pharmaceutical Preservatives physiology Riboflavin Saline Solution Sodium Riboflavin Phosphate Sterility, Reproductive Thiamine Vitamin B6 Vitamins Zinc Sulfate, Heptahydrate

Example 3

Clostridium histolyticum ATCC 21000, strain 004 was inoculated into the starting culture with M #1 or M #2 and incubated at 37° C. for 16 hours. Ten milliliters of the starting culture (M #1 or M #2) and 10 mL Mg/vitamin solution (prepared separately by dissolving 8 g MgSO4, 1.2 g ferrous sulfate, 0.05 g riboflavin, 0.1 g Niacin, 0.1 g Calcium pantothenate, 0.1 g pimelic acid, 0.1 g pyridoxine, and 0.1 g thiamine in 1100 mL water, followed by sterilization by 0.22 μm filtration) was then transferred to each liter of M #3 or M #4 (or a variation thereof), and incubated for 22 hours. Clostridium histolyticum grew well with the OD600 reaching >2.5.

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Patent 2024
Clostridium histolyticum Fermentation ferrous sulfate Filtration Niacin Pantothenate, Calcium Pimelic Acid Pyridoxine Riboflavin Sterilization Strains Sulfate, Magnesium Thiamine Vitamins

Top products related to «Thiamine»

Sourced in United States, Germany, India, Belgium
Thiamine is a laboratory product manufactured by Merck Group. It is a water-soluble vitamin that serves as a cofactor for various enzymatic reactions in the body. Thiamine plays a critical role in the metabolism of carbohydrates, fats, and proteins.
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Riboflavin, also known as vitamin B2, is a water-soluble vitamin that is commonly used in laboratory settings. It serves as a core component in various biological processes, including energy metabolism and cellular respiration. Riboflavin plays a crucial role as a cofactor for enzymes involved in the conversion of food into energy. This product is often used in research and analytical applications where its specific properties and functions are required.
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L-DOPA is a laboratory product manufactured by Merck Group. It is a chemical compound used as a precursor in the synthesis of various pharmaceutical and research-related substances. The core function of L-DOPA is to serve as a starting material or intermediate in chemical reactions and processes. No further details or interpretations are provided.
Sourced in United States, Germany, Italy, United Kingdom, China, Canada, France, Belgium, Japan, India
Biotin is a laboratory-grade product manufactured by Merck Group. It is a water-soluble vitamin that serves as a cofactor for certain enzymes involved in carboxylation reactions. Biotin is commonly used in various biochemical and molecular biology applications.
Sourced in United States, Germany, Spain, Netherlands
Thiamine hydrochloride is a water-soluble chemical compound that serves as a source of vitamin B1 (thiamine). It is commonly used in various laboratory applications, including as a nutrient supplement in cell culture media and as a component in biochemical assays and analyses.
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Chloramphenicol is a bacteriostatic antibiotic that inhibits protein synthesis in bacteria. It is commonly used in microbiology laboratories for selective cultivation and identification of bacterial species.
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Glucose is a laboratory equipment used to measure the concentration of glucose in a sample. It is a fundamental tool in various medical and scientific applications, including the diagnosis and monitoring of diabetes, metabolic research, and food analysis.
Sourced in United States, Austria
Select agar is a solidifying agent used in the preparation of culture media for the growth and isolation of microorganisms. It is derived from red seaweed and provides a stable, inert, and transparent matrix for the cultivation of various microbes.
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KH2PO4 is a chemical compound commonly used as a laboratory reagent. It is a white, crystalline solid with the chemical formula KH2PO4. The compound is soluble in water and is a source of potassium (K) and phosphate (PO4) ions.
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Ampicillin is a broad-spectrum antibiotic used in laboratory settings. It is a penicillin-based compound effective against a variety of gram-positive and gram-negative bacteria. Ampicillin functions by inhibiting cell wall synthesis, leading to bacterial cell lysis and death.

More about "Thiamine"

Thiamine, also known as vitamin B1, is an essential nutrient that plays a crucial role in the proper functioning of the human body.
This water-soluble vitamin is vital for energy production, nerve function, and carbohydrate metabolism.
Deficiency in thiamine can lead to serious health issues, such as beriberi and Wernicke-Korsakoff syndrome.
Researchers utilize advanced tools like PubCompare.ai to optimize their thiamine studies.
This AI-driven platform empowers users to locate the best protocols and products, enhancing the reproducibility and accuracy of their research.
By comparing data-driven insights, researchers can make informed decisions that eleviate their thiamine investigations and advance the understanding of this vital micronutrient.
Beyond thiamine, other B-complex vitamins like riboflavin (vitamin B2) and biotin (vitamin B7) also play important roles in metabolism and overall health.
The combination of these essential nutrients, along with compounds like L-DOPA (a precursor to dopamine), can have synergistic effects on the body's physiological processes.
In the lab, thiamine research may involve the use of thiamine hydrochloride, a common form of the vitamin, as well as other compounds like chloramphenicol, glucose, and select agar media.
The careful selection and optimization of these materials and protocols, facilitated by tools like PubCompare.ai, can help researchers achieve more reliable and meaningful results.
OtherTerms: vitamin B1, energy production, nerve function, carbohydrate metabolism, beriberi, Wernicke-Korsakoff syndrome, PubCompare.ai, riboflavin, biotin, L-DOPA, thiamine hydrochloride, chloramphenicol, glucose, select agar, KH2PO4, ampicillin