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Absorbent Pads
Absorbent Pads
Absorbent Pads are devices designed to absorb and contain liquids, often used for medical, hygiene, or industrial applications.
These pads typically consist of absorbent materials such as cellulose, polymers, or other fibers that can quickly soak up and retain fluids.
They play a crucial role in managing incontinence, wound care, and spill containment, among other uses.
Researchers studying absorbent pad materials, designs, and performance can leverage PubCompare.ai's AI-driven platform to optimize their research protocols and enhance reproducibility.
The platform helps locate relevant protocols from literature, pre-prints, and patents, and provides AI-driven comparisons to identify the best protocols and products, taking the guesswyork out of the research process.
These pads typically consist of absorbent materials such as cellulose, polymers, or other fibers that can quickly soak up and retain fluids.
They play a crucial role in managing incontinence, wound care, and spill containment, among other uses.
Researchers studying absorbent pad materials, designs, and performance can leverage PubCompare.ai's AI-driven platform to optimize their research protocols and enhance reproducibility.
The platform helps locate relevant protocols from literature, pre-prints, and patents, and provides AI-driven comparisons to identify the best protocols and products, taking the guesswyork out of the research process.
Most cited protocols related to «Absorbent Pads»
Absorbent Pads
Brain
Catheters
Claustrophobia
ECHO protocol
fMRI
Head
Human Body
Medulla Oblongata
Neoplasm Metastasis
Patients
Physical Examination
Radionuclide Imaging
Rectum
Saline Solution
Sterility, Reproductive
Urinary Bladder
Urodynamics
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Absorbent Pads
ACE2 protein, human
anti-IgG
Antibodies
Antibodies, Neutralizing
Biological Assay
BLOOD
Buffers
Diagnosis
Gold
Immunoglobulins
Mice, House
Nitrocellulose
Serum
Syncope
Tissue, Membrane
Tissue Donors
Colloidal gold nanoparticles with a mean particle diameter of 40 nm were used to produce antibody-colloidal gold probes in our study. Then, well-dispersed colloidal gold particles were conjugated with anti-BTX-1 mAb. There are four parts in the strip test, including the sample, conjugate, absorbent pads, and nitrocellulose (NC) membrane with test and control zones [24 (link)]. The BTX-1-BSA conjugate was coated onto the Millipore 135 NC membrane as a test line, and HRP-labeled rabbit anti-mouse IgG antibody was coated onto the Millipore 135 NC membrane as a control line. Colloidal gold-antibody conjugates were applied on the treated conjugate pad at a proper spray rate. Finally, four sections of the strip were assembled and stored at room temperature until use.
The competitive immunoassay was performed on the strip test to identify the cross-activity of the colloid gold strip. Different kinds of toxins including BTX-1, BTX-2, BTX-3, okadaic acid (OA), domoic acid (DA), saxitoxin (STX), tetrodotoxin (TTX), and conopeptide (CTX) were used to react with the colloidal gold-BTX-1 mAb conjugate which was pipetted onto the conjugate pad. The detection results could be observed by the naked eye after the mixtures moved forward to the nitrocellulose membrane for incubation for 10 min at room temperature. To evaluate the sensitivity of the strip test, different concentrations of BTX-1 were applied to the sample pad of individual test strips, so that they would flow along the nitrocellulose strip, and then a visible limit of detection (LOD) that resulted in the disappearance of a red band on the test line would be determined [25 (link)].
The competitive immunoassay was performed on the strip test to identify the cross-activity of the colloid gold strip. Different kinds of toxins including BTX-1, BTX-2, BTX-3, okadaic acid (OA), domoic acid (DA), saxitoxin (STX), tetrodotoxin (TTX), and conopeptide (CTX) were used to react with the colloidal gold-BTX-1 mAb conjugate which was pipetted onto the conjugate pad. The detection results could be observed by the naked eye after the mixtures moved forward to the nitrocellulose membrane for incubation for 10 min at room temperature. To evaluate the sensitivity of the strip test, different concentrations of BTX-1 were applied to the sample pad of individual test strips, so that they would flow along the nitrocellulose strip, and then a visible limit of detection (LOD) that resulted in the disappearance of a red band on the test line would be determined [25 (link)].
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Absorbent Pads
anti-IgG
domoic acid
Gold Colloid
Hypersensitivity
Immunoassay
Immunoglobulins
Mice, House
Nitrocellulose
Okadaic Acid
Rabbits
Tetrodotoxin
Tissue, Membrane
Toxins, Biological
Absorbent Pads
Catheters
fMRI
Head
Human Body
Movement
Neoplasm Metastasis
Patients
Protein Biosynthesis
Radionuclide Imaging
Rectum
Saline Solution
Sterility, Reproductive
Urinary Bladder
Urination
Urodynamics
Volume, Residual
Absorbent Pads
Biological Assay
Circadian Rhythms
Dry Ice
Filtration
Freezing
Hormones
Hydrocortisone
Medical Devices
Methyl Green
Polypropylenes
Saliva
Viscosity
Most recents protocols related to «Absorbent Pads»
The capture antibody was diluted to 1 mg/mL in 1 mM phosphate buffer.
The anti-IL-6 antibody (L152) was dispensed onto the NC membrane using
a BioDot (ZX1010) dispensing platform at a flow rate of 1 μL/cm
to obtain a test line width of 1 mm. Then, the NC membrane was dried
in an oven at 37 °C for 1 h. The simplified half dipstick LFD
was then assembled by placing the NC membrane on the backing card
followed by the absorbent pad with a 5 mm overlap on the NC membrane.
Finally, strips were cut 5 mm wide before use.
The anti-IL-6 antibody (L152) was dispensed onto the NC membrane using
a BioDot (ZX1010) dispensing platform at a flow rate of 1 μL/cm
to obtain a test line width of 1 mm. Then, the NC membrane was dried
in an oven at 37 °C for 1 h. The simplified half dipstick LFD
was then assembled by placing the NC membrane on the backing card
followed by the absorbent pad with a 5 mm overlap on the NC membrane.
Finally, strips were cut 5 mm wide before use.
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Absorbent Pads
Antibodies, Anti-Idiotypic
Buffers
Immunoglobulins
LINE-1 Elements
Phosphates
Tissue, Membrane
Purified anti-spike protein monoclonal antibodies (SpMA-01 and SpMA-02) were diluted in 50 mM PBS buffer (pH 7.4), 2 µg of capture mAb (SpMA-02) and 1 µg of goat anti-mouse antibody (Bethyl Lab, USA) and dropped onto a nitrocellulose membrane at the reading window to give the T and C, respectively. To 10 µL of colloidal gold conjugated anti-spike mAb (SpMA-01), an equal volume of 10% alkali-treated casein was mixed and placed onto a conjugate pad. The membranes were then dried at room temperature to immobilize antibodies. Samples of SARS-CoV-2 and its variants, or viruses and bacteria from the FDA pathogen panel were either diluted in PBS or treated with 100 mM TERGITOL-NP (prepared by mixing 334μL 100 mM Tergitol NP-9 with 666μL 100 mM Tergitol NP-10) followed by dilution with 150 µL of PBS. The samples were then placed onto sample application wells. Driven by capillary forces, the immunocomplex migrated up the membrane into the absorbent pad and after 10 to 15 minutes, the test results were evaluated visually. The selection of the optimal concentrations of the spike protein or pathogen antigens were visually inspected for Test (T-) and Control (C-) results. To determine the analytical sensitivity of spike protein in saliva or nasal samples, 200 ng of recombinant (S) was spiked into swabs and a 150μL extract was tested in the assay.
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Absorbent Pads
Alkalies
Anti-Antibodies
Antibodies
Antibodies, Anti-Idiotypic
Antigens
Bacteria
Biological Assay
Buffers
Capillaries
Caseins
Goat
Gold Colloid
Hypersensitivity
Immobilization
Mice, House
M protein, multiple myeloma
Nitrocellulose
Nose
NP 10
Pathogenicity
S-phenyl-N-acetylcysteine
Saliva
SARS-CoV-2
Technique, Dilution
Tergitol
Tissue, Membrane
Virus
The performances of the strips composed of the NC membrane, the single-layer conjugate pad, and the absorbent pad were tested. The strips were manually cut into 6 × 0.5 cm and housed in a plastic cassette. The test and control lines were spotted manually in a circle by spotting 2 µg/dot of anti-spike mAb (SpMA-02) and 1 µg/dot of goat anti-mouse antibody (I-0759, Sigma, USA) at the reading window to give the test (T) and control line (C), respectively. To 10 µL of colloidal gold conjugated anti-spike mAb (SpMA-01), an equal volume of 10% alkali-treated casein was mixed and placed onto a conjugate pad. The membranes were dried for 5 to 7 minutes at room temperature to immobilize antibodies. Different concentrations of recombinant S ranging from 200 ng to 12.5 ng in 150 µL buffer and the control Vero cell extract [200 ng in 150 µL of PBS (50 mM PBS, pH 7.4)] were placed onto the sample application point. Driven by capillary forces, the immunocomplex migrated up the membrane into the absorbent pad and the test results were evaluated visually after 10 -15 minutes, the test results were evaluated visually. The selection of the optimal concentrations of the spike protein was visually inspected for T and C line results. The limit of detection (LOD) was calculated accordingly.
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Absorbent Pads
Alkalies
Antibodies
Antibodies, Anti-Idiotypic
Buffers
Capillaries
Caseins
Cell Extracts
Goat
Gold Colloid
Immobilization
M protein, multiple myeloma
Mus
S-phenyl-N-acetylcysteine
Tissue, Membrane
The LFIA strips were assembled using nitrocellulose (NC) membrane fixed on 10 mils polystyrene backing with high protein binding capacity (FF80HP plus LAM, Cytiva, USA), with pore sizes of 12 and 15 µm; and wicking time of 60 to 100 seconds. A single-layer matrix (Fusion-5, Cytiva, USA) was used as the sample and conjugate pad without any specific pre-treatment for uniform movement of gold nanoparticle conjugates. The absorbent pad (CF 7 Cytiva, USA), with high absorbance capacity, 100% cotton linter material (1873 µm thickness at 53kPA, Cytiva, USA) was overlapped at the top on the NC membrane.
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Absorbent Pads
Gold
Gossypium
Maternally Inherited Leigh Syndrome
Movement
Neoplasm Metastasis
Nitrocellulose
Polystyrenes
Tissue, Membrane
Forced swimming stress model was first introduced by Porsolt et al. [25 (link)], in 1977. The procedure for this model is to place the mouse for 6 minutes into a glass-polycarbonate cylinder (25 cm high×10 cm wide) filled to a depth of 10cm with water maintained at 24°C. Immediately following the 6 minutes exposure into the water cylinder, the animals are removed from the cylinder and dried using a water absorbent pad. In the current study, we used a recently described five day repeated forced swim stress (5d-RFSS) protocol [22 (link)], in which mice were forced to swim in an open cylindrical container (diameter, 10 cm; height, 25 cm) containing 10 cm deep water (25°C) for five consecutive days. The five days procedure constituted the induction phase of the stress model. This protocol had been shown to produce the depressive symptoms that last for at least four weeks, allowing for a time window to test and study therapeutic interventions [22 (link)]. After the five days induction period, animals were treated for 21 days, while five minutes behavioural tests were conducted and video recorded at intervals on days 7, 14 and 21, after 5d-RFSS. The period/duration of swimming and immobility during the 6 minutes test was measured from the video recordings and recorded using a timer. While the FST has received extensive criticism regarding its validity and reproducibility, a metanalysis of 73 behavioral studies that used FST in mice demonstrated external validity and reproducibility of the FST where different types of antidepressant drugs reduced immobility in the test [26 (link)].
The issue of ethics regarding the use of the FST has been raised due to its perceived suffering to animals [27 ]. However, there is currently no regulation against the use of the FST. The fact sheet published by British Association for Psychopharmacology in conjunction with, the Laboratory Animal Science Association and Understanding Animal Research advocated for the continued use of FST as a valuable behavioral screening tool [28 ]. A recent commentary suggested the use of none-behavioral neurochemical measures like brain derived neurotropic factor (BDNF) as an alternative to the FST [29 (link)]. The current study included the testing of the BDNF levels in the brain and showed that the changes in the BDNF are in solidarity with the reduction in immobility time in the FST paving a way for a shift away from the FST.
The issue of ethics regarding the use of the FST has been raised due to its perceived suffering to animals [27 ]. However, there is currently no regulation against the use of the FST. The fact sheet published by British Association for Psychopharmacology in conjunction with, the Laboratory Animal Science Association and Understanding Animal Research advocated for the continued use of FST as a valuable behavioral screening tool [28 ]. A recent commentary suggested the use of none-behavioral neurochemical measures like brain derived neurotropic factor (BDNF) as an alternative to the FST [29 (link)]. The current study included the testing of the BDNF levels in the brain and showed that the changes in the BDNF are in solidarity with the reduction in immobility time in the FST paving a way for a shift away from the FST.
Absorbent Pads
Adult Fanconi Syndrome
Animals
Antidepressive Agents
Behavior Test
Brain
Depressive Symptoms
Mice, Laboratory
polycarbonate
Therapeutics
Top products related to «Absorbent Pads»
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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Tween 20 is a non-ionic detergent commonly used in biochemical applications. It is a polyoxyethylene sorbitan monolaurate, a surfactant that can be used to solubilize and stabilize proteins and other biomolecules. Tween 20 is widely used in various laboratory techniques, such as Western blotting, ELISA, and immunoprecipitation, to prevent non-specific binding and improve the efficiency of these assays.
Sourced in United States, China
Absorbent pads are laboratory equipment designed to absorb and contain liquids. They are made of highly absorbent materials and are used to protect surfaces from spills and leaks during various laboratory procedures.
Sourced in China
Biotin-BSA is a conjugate of biotin and bovine serum albumin (BSA). Biotin is a small molecule vitamin that can form a strong, non-covalent interaction with the protein avidin and its derivatives. The Biotin-BSA conjugate can be used as a tool in various biotechnology and biomedical applications that involve biotin-avidin interactions.
Sourced in China
Biotinylated bovine serum albumin (biotin-BSA) is a protein conjugate used as a labeling reagent in various bioassays and research applications. It consists of the protein bovine serum albumin (BSA) covalently linked to the small molecule biotin. The biotin moiety on the conjugate can be utilized to detect or capture target analytes in a sample through high-affinity interactions with streptavidin or avidin.
Sourced in Germany, United States, France, United Kingdom, Netherlands, Spain, Japan, China, Italy, Canada, Switzerland, Australia, Sweden, India, Belgium, Brazil, Denmark
The QIAamp DNA Mini Kit is a laboratory equipment product designed for the purification of genomic DNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify DNA, which can then be used for various downstream applications.
Sourced in United States
Dye (Crimson red) streptavidin-coated polymer nanoparticles are a type of lab equipment produced by Bangs Laboratories. These nanoparticles have a core function of providing a fluorescent dye label with a crimson red color. The nanoparticles are coated with streptavidin, a protein that can bind to biotinylated molecules.
Sourced in United States, Germany, United Kingdom, France, Switzerland, Sao Tome and Principe, China, Macao, Italy, Poland, Canada, Spain, India, Australia, Belgium, Japan, Sweden, Israel, Denmark, Austria, Singapore, Ireland, Mexico, Greece, Brazil
Sucrose is a disaccharide composed of glucose and fructose. It is commonly used as a laboratory reagent for various applications, serving as a standard reference substance and control material in analytical procedures.
Sourced in United States, Germany, China, United Kingdom, Ireland, France, Morocco, Italy, Australia, Sao Tome and Principe, Canada, Macao, Spain, India
Nitrocellulose membranes are a type of laboratory equipment used for various applications, such as protein blotting, DNA/RNA transfer, and immunodetection. They are made from purified cellulose that has been chemically treated to create a porous structure, allowing efficient binding and transfer of biomolecules. These membranes provide a reliable and versatile platform for a range of research and analytical procedures.
Sourced in United States, China, Germany
Nitrocellulose (NC) membrane is a porous sheet-like material made from nitrated cellulose. It is commonly used as a support matrix in various analytical techniques, such as Western blotting, dot blotting, and nucleic acid hybridization assays. The membrane's high binding capacity and ability to immobilize proteins, nucleic acids, and other biomolecules make it a widely used tool in biochemistry and molecular biology laboratories.
More about "Absorbent Pads"
Absorbent pads, also known as sanitary pads or incontinence pads, are essential devices designed to absorb and contain various types of liquids.
These pads typically consist of absorbent materials such as cellulose, polymers, or other fibers that can quickly soak up and retain fluids.
They play a crucial role in managing incontinence, wound care, and spill containment, among other medical, hygiene, and industrial applications.
Researchers studying absorbent pad materials, designs, and performance can leverage PubCompare.ai's AI-driven platform to optimize their research protocols and enhance reproducibility.
The platform helps locate relevant protocols from literature, pre-prints, and patents, and provides AI-driven comparisons to identify the best protocols and products, taking the guesswork out of the research process.
Bovine serum albumin (BSA) and Tween 20 are commonly used in absorbent pad research, often in conjunction with biotinylated BSA (biotin-BSA) and dye-labeled streptavidin-coated polymer nanoparticles.
The QIAamp DNA Mini Kit is another tool that may be employed in absorbent pad studies, while sucrose and nitrocellulose (NC) membranes can also play a role in the research.
By leveraging PubCompare.ai's AI-driven platform and incorporating these related terms and materials, researchers can streamline their work, enhance reproducibility, and optimize their absorbent pad research protocols, leading to more efficient and effective studies.
These pads typically consist of absorbent materials such as cellulose, polymers, or other fibers that can quickly soak up and retain fluids.
They play a crucial role in managing incontinence, wound care, and spill containment, among other medical, hygiene, and industrial applications.
Researchers studying absorbent pad materials, designs, and performance can leverage PubCompare.ai's AI-driven platform to optimize their research protocols and enhance reproducibility.
The platform helps locate relevant protocols from literature, pre-prints, and patents, and provides AI-driven comparisons to identify the best protocols and products, taking the guesswork out of the research process.
Bovine serum albumin (BSA) and Tween 20 are commonly used in absorbent pad research, often in conjunction with biotinylated BSA (biotin-BSA) and dye-labeled streptavidin-coated polymer nanoparticles.
The QIAamp DNA Mini Kit is another tool that may be employed in absorbent pad studies, while sucrose and nitrocellulose (NC) membranes can also play a role in the research.
By leveraging PubCompare.ai's AI-driven platform and incorporating these related terms and materials, researchers can streamline their work, enhance reproducibility, and optimize their absorbent pad research protocols, leading to more efficient and effective studies.