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Forceps

Forcepse are medical instruments used to grasp, hold, or manipulate objects during surgical procedures.
They come in a variety of shapes and sizes to meet different clinical needs, such as obstetric forceps for assisting with difficult childbirths, or surgical forceps for grasping and manipulating tissues.
Forceps play a critical role in many medical interventions, helping to improve outcomes and enhance patient safety.
Researchers can use PubCompare.ai to quickly identify the most reliable and effective forceps products by comparing protocols from literature, preprints, and patents using advanced AI-driven analysis.
This can help optimize forceps research, enhance reproducibility, and ensure accuracy in clinical applications.

Most cited protocols related to «Forceps»

A schematic overview of the myocyte isolation procedure is shown in Figure 2. An expanded description of the procedure, accompanied with images and videos, and complete materials list is available in the Online Data Supplement, alongside full details of additional methods applied in this study (Appendix A-ix). All animal work was undertaken in accordance with Singapore National Advisory Committee for Laboratory Animal Research guidelines. Relevant national and institutional guidelines and regulations must be consulted before commencement of all animal work.
Buffers and media were prepared as detailed in Appendix D. EDTA, perfusion, and collagenase buffers were apportioned into sterile 10 mL syringes, and sterile 27 G hypodermic needles were attached (Online Figure IA).
C57/BL6J mice aged 8 to 12 weeks were anesthetized, and the chest was opened to expose the heart. Descending aorta was cut, and the heart was immediately flushed by injection of 7 mL EDTA buffer into the right ventricle. Ascending aorta was clamped using Reynolds forceps, and the heart was transferred to a 60-mm dish containing fresh EDTA buffer. Digestion was achieved by sequential injection of 10 mL EDTA buffer, 3 mL perfusion buffer, and 30 to 50 mL collagenase buffer into the left ventricle (LV). Constituent chambers (atria, LV, and right ventricle) were then separated and gently pulled into 1-mm pieces using forceps. Cellular dissociation was completed by gentle trituration, and enzyme activity was inhibited by addition of 5 mL stop buffer.
Cell suspension was passed through a 100-μm filter, and cells underwent 4 sequential rounds of gravity settling, using 3 intermediate calcium reintroduction buffers to gradually restore calcium concentration to physiological levels. The cell pellet in each round was enriched with myocytes and ultimately formed a highly pure myocyte fraction, whereas the supernatant from each round was combined to produce a fraction containing nonmyocyte cardiac populations.
CM yields and percentage of viable rod-shaped cells were quantified using a hemocytometer. Where required, the CMs were resuspended in prewarmed plating media and plated at an applicationdependent density, onto laminin (5 μg/mL) precoated tissue culture plastic or glass coverslips, in a humidified tissue culture incubator (37°C, 5% CO2). After 1 hour, and every 48 hours thereafter, media was changed to fresh, prewarmed culture media.
The cardiac nonmyocyte fraction was collected by centrifugation (300g, 5 minutes), resuspended in fibroblast growth media, and plated on tissue-culture treated plastic, area ≈ 23 cm2 (0.5× 12-well plate) per LV, in a humidified tissue culture incubator. Media was changed after 24 hours and every 48 hours thereafter.
Publication 2016
Animals Animals, Laboratory Ascending Aorta Buffers Calcium Centrifugation Chest Collagenase Culture Media Descending Aorta Dietary Supplements Digestion Edetic Acid enzyme activity Fibroblasts Forceps Gravity Heart Heart Atrium Hyperostosis, Diffuse Idiopathic Skeletal Hypodermic Needles isolation Laminin Left Ventricles Mus Muscle Cells Perfusion physiology Population Group Retreatments Rod Photoreceptors Sterility, Reproductive Syringes Tissues Ventricles, Right
Brains were harvested whole from P14 or P25 mice on a 129 background and stained using the FD Rapid GolgiStain kit (FD NeuroTechnologies). Brains were rinsed with double distilled water and then immersed in a 1∶1 mixture of FD Solution A∶B for 2 weeks at room temperature in the dark. Brains were then transferred to FD Solution C and kept in the dark at 4°C for 48 hours. Solution C was replaced after the first 24 hours. In preparation for freezing, individual brains were placed in Peel-A-Way disposable embedding molds (VWR) and immersed in Tissue Freezing Medium (Triangle Biomedical Sciences). Dry ice was used to line the bottom of an ice bucket, which was then filled with 190-proof ethanol (Koptec). Using forceps, the molds were lowered into the ethanol (being careful not to allow the ethanol to spill into the top of the mold) and held until the TFM froze. Brains were kept at −80°C until sectioning. Cryosectioning was performed on a Leica CM 3050 S at −22°C. Coronal sections of 100 µm thickness were cut and transferred to gelatin coated slides (LabScientific) onto small drops of FD Solution C. This thickness enabled optimal staining and preservation of spines on the secondary and tertiary dendritic segments used for analysis in the examples presented here. However, any thickness between 80 to 240 µm (recommended in the FD Rapid GolgiStain instructions) can be used in order to satisfy the user’s unique requirements, providing that individual dendritic spines can still be differentiated and measured. After allowing sections to dry at room temperature in the dark for at least 4 hours (or overnight), slides were then stained exactly as described in the FD Rapid GolgiStain instructions (under “Part VI. Staining Procedure”). Permount (Fisher) was used for coverslipping.
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Publication 2014
ARID1A protein, human Biologic Preservation Brain Dendrites Dendritic Spines Dry Ice Ethanol Forceps Freezing Fungus, Filamentous Gelatins Mice, 129 Strain Tissues Vertebral Column

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Publication 2013
Acoustics Acoustic Stimulation Animals Cortex, Cerebral Dissection DNA Chips Food Forceps Fostex Hypersensitivity isolation Ketamine Light Medulla Oblongata Mice, House Pharmaceutical Preparations Photic Stimulation Pressure Sound Tamoxifen Vibrissae Xylazine
Five minutes after injecting with Avertin, animals were checked for responses and euthanized by cervical dislocation. Eyes were immediately enucleated and kept in ice-cold medium before dissection. The choice of the medium was identical to the one used for incubation later. After removing the cornea and the lens from the anterior of the eye, the central or peripheral choroid-scleral complex was separated from the retina and cut into approximately ∼2 mm×1 mm pieces (rats) or 1 mm×1 mm (mice). Choroid/sclera (here on referred to as “choroid”) fragments were isolated with and without RPE removal by peeling RPE away with forceps and placed in growth factor-reduced Matrigel™ (BD Biosciences, Cat. 354230) seeded in 24 well plates (Supporting Video S1). 30 µL of matrigel was used to coat the bottom of 24 well plates without touching the edge of the well. The thickness of the matrigel was approximately 0.4 mm. After seeding the choroid, plates were incubated in a 37°C cell culture incubator without medium for 10 minutes in order for the Matrigel™ to solidify. 500 µL of medium was then added to each well and incubated at 37°C with 5% CO2 for 48 hr before any treatment. Medium was changed every 48 hr. Phase contrast photos of individual explants were taken daily using a ZEISS Axio Oberver.Z1 microscope. The areas of sprouting were quantified with computer software ImageJ 1.46r (National Institute of Health). The macro for SWIFT-Choroid quantification is available from the authors.
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Publication 2013
Animals Cell Culture Techniques Choroid Cold Temperature Cornea Dissection Eye Forceps Growth Factor Joint Dislocations Lens, Crystalline matrigel Microscopy Microscopy, Phase-Contrast Mus Neck Rattus Retinal Detachment Sclera tribromoethanol
Spleens from 6–10-wk-old female C57BL/6 mice were used. Single cell suspensions were obtained by gentle teasing with forceps and filtered with a cell strainer. Erythrocytes were lysed by treatment with ammonium chloride. Remaining unfractionated cell populations were plated at a density of 5 × 105cells/ml in suspension culture Petri dishes (Corning Glass Works, Corning, NY). Culture medium for generation and expansion of DC was complete IMDM supplemented with 30% NIH/3T3 SN containing 10–20 ng/ml mouse rGM-CSF (we refer to this conditioned medium as R1 medium). Cultures were fed with fresh R1 medium every 3–4 d. First passages of DC-enriched cultures were performed after ∼2 wk. Both suspended and weakly adherent cells were propagated. Clusters of adherent cells with dendritic morphology were detached using PBS with 3 mM EDTA. The remaining strongly adherent cells were discarded. After 3 mo of culture, cells could be passaged every week. Experiments were performed with cells continuously cultured for more than 12 mo. This homogeneous, growth factor–dependent DC population, is referred to as D1 cells.
In experiments using rTNFα, culture medium was changed before the experiment and fresh rTNFα (100 U/ml) was added every 24 h. Similarly, rM-CSF and rGM-CSF were used at a concentration of 20 ng/ml.
Publication 1997
Cells Chloride, Ammonium Culture Media Culture Media, Conditioned Dendrites Edetic Acid Erythrocytes Females Forceps Hyperostosis, Diffuse Idiopathic Skeletal Mice, Inbred C57BL Mus NIH 3T3 Cells Population Group sargramostim

Most recents protocols related to «Forceps»

Adult ticks observed and counted at day 6 and day 8 post-exposure were defined by attachment status (attached, detached) and feeding status (non-engorged, partially engorged, fully engorged), with ‘attached’ = adults which remain imbedded in the skin of deer; ‘detached’ = adults which are not imbedded in the skin of deer; ‘flat’ = non-engorged adults, showing no discernable blood meal; ‘partially engorged’ = adults with partial blood meal discernable, but not fully fed; and ‘fully engorged’ = completely bloated and darkly colored adults. Ticks were further defined by condition (dead, alive), which was determined by carefully observing and manipulating attached and detached ticks with fine-tipped forceps to elicit movement, with ‘alive’ = movement of legs, palps or mouthparts; and ‘dead’ = no movement after approximately 45 s of manipulation. The attachment status, feeding status and condition of female ticks were compared between treatment and control groups. The proportion of ticks recovered within each test group was also investigated. Differences in the proportion of ticks attached and detached for each species and differences in feeding status and condition of each species within each test group were compared using a Pearson’s χ2 test for independence.
The weights of engorged females and approximate number of eggs and hatched larvae were compared between the treatment and control groups. To estimate the approximate number of eggs in each egg mass, an assumption was made that 1 g of ixodid eggs would contain approximately 20,000 individual eggs [46 (link)]. The number of hatched larvae was estimated by multiplying the approximate proportion of hatched eggs by the approximate number of eggs [46 (link)]. Differences in the weights of engorged females detaching from FDF-treated deer, relative to deer in the control group, and the subsequent numbers of eggs and larvae produced per female were estimated using a Student’s t-test.
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Publication 2023
Adult BLOOD Deer Eggs Females Forceps Larva Leg Movement Skin Student Ticks
Preterm birth was the primary outcome of this study, which was defined as births before 37 completed weeks of gestation. The World Health Organization (WHO) further subdivided preterm birth based on gestational age: extremely preterm (< 28 weeks), very preterm (28 to < 32 weeks), and moderate or late preterm (32 to < 37 weeks) [23 (link)]. Secondary outcomes were NICU admission, low birthweight and small for gestational age. Low birthweight was defined as a birthweight < 2500 g, and small for gestational age was defined as a birthweight less than the 10th percentile. The following variables were collected: maternal age at delivery (years), race [Asian, Black (Black or African American), White, other (American Indian or Alaska Native, Native Hawaiian or Other Pacific Islander, and more than one race)], education [less than 12 grade, high school/general educational development (GED), some college or associate degree (AA), bachelor or higher], pre-pregnancy weight (lb), pre-pregnancy body mass index (BMI) (BMI < 18.5 kg/m2, underweight; BMI = 18.5–24.9 kg/m2, normal; BMI = 25.0–29.9 kg/m2, overweight; BMI = 30.0–34.9 kg/m2, obesity), delivery weight (lb), weight gain (lb), smoking before pregnancy (yes or no), smoking status 1st/2nd/3rd trimester (mother-reported smoking in the three trimesters of pregnancy, yes or no), hypertension eclampsia (yes or no), gestational hypertension (yes or no), pre-pregnancy hypertension (yes or no), number of prenatal visits, the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC, receipt of WIC food for the mother during this pregnancy, yes or no), plurality, prior birth now living, prior birth now dead, prior other terminations, total birth order, gestational age (weeks), newborn sex (female or male), birth weight (g), infertility treatment used (yes or no), pregnancy method (natural pregnancy, pregnancy via ART), method of delivery [spontaneous, non-spontaneous (forceps, vacuum, cesarean)], preterm birth [extremely preterm, very preterm, moderate or late preterm; spontaneous, indicated (forceps, vacuum, cesarean)], NICU admission, low birthweight (yes or no), and small for gestational age (yes or no). WIC is a program intended to help low income pregnant women, infants, and children through age 5 receive proper nutrition by providing vouchers for food, nutrition counseling, health care screenings and referrals; it is administered by the U.S. Department of Agriculture (https://ftp.cdc.gov/pub/Health_Statistics/NCHS/Dataset_Documentation/DVS/natality/UserGuide2019-508.pdf). Infertility treatment referred to using fertility enhancing drugs, artificial insemination, intrauterine insemination, or using ART. ART included in vitro fertilization (IVF), gamete intrafallopian transfer (GIFT), and zygote intrafallopian transfer (ZIFT). Information on variables is available at https://www.cdc.gov/nchs/nvss/index.htm.
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Publication 2023
African American Alaskan Natives American Indians Artificial Insemination Asian Americans Birth Birth Weight Child Eclampsia Fertility Agents Fertilization in Vitro Food Forceps Gamete Intrafallopian Transfer Gestational Age High Blood Pressures Index, Body Mass Infant Infant, Newborn Insemination Males Mothers Native Hawaiians Obesity Obstetric Delivery Pacific Islander Americans Pregnancy Pregnant Women Prehypertension Premature Birth Screening Sterility, Reproductive Transient Hypertension, Pregnancy Vacuum Woman Zygote Intrafallopian Transfer
The experiment ended after 4 groups of rats were fed in the artificial climate simulator for 1 month. After the experiment ended, 3 rats were randomly selected from each group and their perianal skin was disinfected with 75% alcohol. The feces of each rat was collected using sterilized forceps and placed into a sterile 2-mL Eppendorf tube. The collected feces were immediately stored in an ultra-low temperature refrigerator at −80°C.
The microbial community genomic total DNA was extracted by E.Z.N.A. ® Stool DNA Kit (Omega Bio-Tek, United States). PCR amplification was performed using 16S V4 region primers (515F and 806R) according to the requirement of Phusion® High-Fidelity PCR Master Mix amplification kit (New England Biolabs). The purity of the PCR amplified sample was tested by agarose gel electrophoresis. After passing the test, the instructions of the TruSeq® DNA PCR-Free Sample Preparation Kit (New England Biolabs) were followed to build a sequencing library. After building and testing the library, the NovaSeq6000 sequencing platform was used by Beijing Novogene Technology Co., Ltd., to perform the sequencing of the flora. After sorting out the original data, effective tags were clustered, and the sequences were clustered into operational taxonomic units (OTUs) with 97% consistency. Then, using the representative sequences of OTUs, species annotation (Venn Graph, species relative abundance column accumulation map, species abundance cluster heat map), alpha diversity analysis (Shannon index, Chao1 index), beta diversity analysis (principal co-ordinate analysis, PCoA), and between-group difference species analysis (LDA effect size analysis) were performed.
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Publication 2023
Climate Cold Temperature DNA Library Electrophoresis, Agar Gel Ethanol Feces Forceps Genome Microbial Community Oligonucleotide Primers Skin Sterility, Reproductive
There was no difference in surgical procedure between the ASS group and HS group except for skin closure. For the ASS group, we used Insorb (Incisive Surgical, Inc.), which is a brand name of absorbable subcuticular skin stapler. First, the operator grasps both edges of the skin together with a tooth forceps using 1 hand, while the other hand holds the stapler. After locating the nose of the stapler underneath the grasped tissue, the operator fires the staple. This process is repeated along the incision till the opposite end, and staples are placed at about 7-mm intervals. Fig. 2 shows a mastectomy field from our center in which an ASS was being applied. For the HS group, interrupted subcuticular suture was done using Monosyn (B. Braun). First-generation cephalosporin was injected once on the day of the operation as a prophylactic antibiotic into every patient, and no additional antibiotics were used postoperatively. Only for the patients in whom SSI was detected was an additional third-generation cephalosporin plus metronidazole used empirically; or in cases of positive culture from the wound, other proper antibiotics were administered according to the result of the culture.
Publication 2023
Antibiotics Antibiotics, Antitubercular Cephalosporins Condoms Fires Forceps Mastectomy Metronidazole Nose Operative Surgical Procedures Patients Skin Staple, Surgical Surgery, Day Sutures Tissues Tooth Wounds
Anesthesiologists used neuroleptic sedation for each patient with a combination of ketamine, midazolam, fentanyl and propofol. The surgeon used loupes with a 3.3X magnification and a headlight. The tumour was assessed, measured (Figure 1A), and marked with standard four millimetre surgical margins for BCC and seven millimeter surgical margins for melanoma in situ (MIS). The width of the excised area was documented. The donor tissue width was estimated and marked (Figure 1B). The donor lid was then stretched horizontally, ensuring that the secondary defect could undergo direct closure. One drop of topical anesthesia was placed in each eye and the operative site was prepared with controlled use of chlorhexidine to limit the risk of corneal toxicity. The surgeon performed subcutaneous infiltration of the tumor and donor sites using lidocaine 2% with epinephrine 1:100,000; 2 ml or less per eyelid. All tissue was handled with 0.5 mm toothed forceps to preserve its architecture and integrity.
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Publication 2023
Anesthesiologist Antipsychotic Agents Chlorhexidine Cornea Epinephrine Eyelids Fentanyl Forceps Ketamine Lidocaine Melanoma Midazolam Neoplasms Patients Propofol Sedatives Surgeons Surgical Margins Tissue Donors Tissues Topical Anesthetics

Top products related to «Forceps»

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Trypsin is a proteolytic enzyme that hydrolyzes peptide bonds in proteins. It is commonly used in cell biology and molecular biology applications to facilitate cell detachment and dissociation.
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The GIF-Q260J is a laboratory equipment product from Olympus. It is designed to perform a core function, but the specific details of its intended use are not available in this concise, unbiased, and factual description.
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DNase I is a lab equipment product that serves as an enzyme used for cleaving DNA molecules. It functions by catalyzing the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, effectively breaking down DNA strands.
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MS-222 is a chemical compound commonly used as a fish anesthetic in research and aquaculture settings. It is a white, crystalline powder that can be dissolved in water to create a sedative solution for fish. The primary function of MS-222 is to temporarily immobilize fish, allowing for safe handling, examination, or other procedures to be performed. This product is widely used in the scientific community to facilitate the study and care of various fish species.
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RNAlater is a RNA stabilization solution developed by Thermo Fisher Scientific. It is designed to protect RNA from degradation during sample collection, storage, and transportation. RNAlater stabilizes the RNA in tissues and cells, allowing for efficient RNA extraction and analysis.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Dispase II is a highly purified protease enzyme that can be used for the dissociation of a variety of cell types, including epithelial, endothelial, and neural cells. It is an effective tool for the isolation and culture of primary cells from tissues.

More about "Forceps"

Forceps are a crucial medical instrument used in a variety of surgical procedures.
These handheld tools come in a range of shapes and sizes, from obstetric forceps used to assist with difficult childbirths to surgical forceps for grasping and manipulating tissues.
Forceps play a critical role in many medical interventions, helping to improve outcomes and enhance patient safety.
Researchers can utilize advanced AI-driven analysis platforms like PubCompare.ai to quickly identify the most reliable and effective forceps products by comparing protocols from literature, preprints, and patents.
This can help optimize forceps research, enhance reproducibility, and ensure accuracy in clinical applications.
The versatility of forceps makes them an essential tool in the medical field.
Clinicians may use them in conjunction with other instruments like Penicillin/streptomycin, TRIzol reagent, Trypsin, GIF-Q260J, DNase I, MS-222, RNAlater, DMEM, and Dispase II to perform a wide range of procedures.
By utilizing the latest technology and research, healthcare professionals can ensure that forceps are used effectively and safely, ultimately leading to better patient outcomes.
One important aspect of forceps research is the need for clear and reproducible protocols.
PubCompare.ai can help researchers identify the most reliable and effective forceps products by comparing protocols from various sources, including literature, preprints, and patents.
This can lead to enhanced reproducibility and accuracy in clinical applications, which is crucial for patient safety and positive clinical outcomes.
In conclusion, forceps are a vital medical instrument that play a crucial role in many surgical procedures.
By leveraging the power of AI-driven analysis tools like PubCompare.ai, researchers can optimize their forceps research, enhance reproducibility, and ensure the accuracy of clinical applications.
This can ultimately lead to improved patient care and better medical outcomes.