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Neointima

Neointima: A layer of smooth muscle cells and extracellular matrix that forms within the lumen of a blood vessel, typically in response to injury or disease.
This proliferative growth can lead to narrowing of the vessel and impaired blood flow.
Understanding the mechanisms and factors influencing neointima formation is crucial for developing effective treatments for cardiovascular conditions such as restenosis and atherosclerosis.
Researchers can optimize their neointima studies by utilizing PubCompare.ai's AI-powered platform, which helps locate the best protocols from literature, preprints, and patents, while providing detailed comparisons to enhance reproducibility and accuarcy.

Most cited protocols related to «Neointima»

1
Optical Coherence Tomography Imaging in Clinical Practice
The main obstacle to the adoption of TD-OCT imaging in clinical practice is that OCT cannot image through a blood field, and therefore requires clearing or flushing of blood from the lumen.1 (link) The 6 Fr compatible DragonflyTM FD-OCT catheter is so far the only one in the market, as two other systems from Volcano and Terumo, which have function similar to the DragonflyTM, are not yet available. The DragonflyTM catheter is first advanced over a regular guide wire, distal to the region of interest. A dedicated marker, located 10 mm distal to the OCT lens, enables the pull-back starting point selection.
The acquisition of an OCT image sequence requires a bolus of crystalloid solution (usually contrast) injected through the guiding catheter. The acquisition speed can be set up in a range between 5 and 40 mm/s, based on the used OCT system. Most expert users advocate the use of automated contrast injection to optimize the image quality.
The previous experience with TD and FD-OCT technology shows OCT acquisition to be safe,1 (link),4 (link),6 effective,1 (link),4 (link),6 –8 and highly reproducible for the assessment of the luminal areas and length.9 –11 (link) A fair correlation between OCT and IVUS quantitative measurements of the lumen areas was reported,9 –11 (link) despite comparative studies showing that IVUS tends to slightly overestimate lumen areas, while stent and neointimal areas are slightly higher on OCT.9 –11 (link) Frequency domain optical coherence tomography image quality depends on an accurate acquisition technique and proper guiding catheter engagement is needed to optimize directional contrast flushing.
Prati F., Guagliumi G., Mintz G.S., Costa M., Regar E., Akasaka T., Barlis P., Tearney G.J., Jang I.K., Arbustini E., Bezerra H.G., Ozaki Y., Bruining N., Dudek D., Radu M., Erglis A., Motreff P., Alfonso F., Toutouzas K., Gonzalo N., Tamburino C., Adriaenssens T., Pinto F., Serruys P.W, & Di Mario C. (2012). Expert review document part 2: methodology, terminology and clinical applications of optical coherence tomography for the assessment of interventional procedures. European Heart Journal, 33(20), 2513-2520.
Publication 2012
BLOOD Catheters Lens, Crystalline Neointima Phenobarbital POU2F3 protein, human Solutions, Crystalloid Stents Tomography, Optical Coherence
2
Atherosclerosis Development in Coronary Stents

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Publication 2011
Atherosclerosis Blood Platelets carbazole Chromogenic Substrates Fibroatheroma Fibrosis Foam Cells Gills Hematoxylin Immunoglobulins Immunohistochemistry Macrophage Neointima Neointima Formation Ovum Implantation Patients Physiologic Calcification Plaque, Atherosclerotic Senile Plaques Stents Technique, Dilution Thrombosis Thrombus Tunica Intima
3
Genetic Mapping of Cardiovascular Traits
Linkage analyses of neointimal lesions, plasma lipid and MCP-1 levels were performed by using MapManager QTXb20 (http://mapmgr.roswellpark.org/) and J/qtl (http://research.jax.org/faculty/churchill/software/Jqtl/index.html) software. The distributions of trait values in F2 mice were assessed for normality with the SPSS program (SPSS, Chicago) by examining skewness, kurtosis, Kolmogorov-Smirnov test, and Q-Q plots. Neointimal lesion sizes were square root transformed before QTL analysis was performed as they were not normally distributed. Non-HDL cholesterol, triglyceride, and MCP-1 levels were directly used for QTL analysis. One thousand permutations of the trait values were used to define the genome-wide LOD score threshold required to be significant or suggestive for each specific trait. The support interval (SI) for each QTL was determined by using a 1-LOD drop from the QTL peak. Variance and mode of inheritance of the traits were determined with MapManager and further confirmation was made with linear regression analysis. ANOVA was used to determine whether the mean phenotype values of progeny with different genotypes at a specific marker were significantly different. Differences were considered statistically significant at P < 0.05.
Yuan Z., Pei H., Roberts D.J., Zhang Z., Rowlan J.S., Matsumoto A.H, & Shi W. (2009). Quantitative trait locus analysis of neointimal formation in an intercross between C57BL/6 and C3H/HeJ apolipoprotein E-deficient mice. Circulation. Cardiovascular genetics, 2(3), 220-228.
Publication 2009
CCL2 protein, human Faculty Genetic Linkage Analysis Genome Genotype High Density Lipoprotein Cholesterol Lipids Mice, House Neointima neuro-oncological ventral antigen 2, human Pattern, Inheritance Phenotype Plant Roots Plasma Triglycerides
4
Arteriovenous Fistula Mouse Model for Neointimal Hyperplasia
All animal studies and experiments were approved by the University of Alabama at Birmingham Institutional Animal Care and Use Committee (IACUC) and performed in accordance with National Institutes of Health guidelines. Our studies utilized male C57BL/6J mice (n = 3, Taconic Biosciences, Hudson, NY) aged 8-12 weeks.
After mice (n = 2) with AVF were anesthetized with isoflurane, buprenorphine, xyalazine, and ketamine, a midline incision of the surgical area was performed. Using a surgical microscope, the right carotid artery and jugular vein were then exposed. Using 10-0 monofilament microsurgical sutures, a side-to-end anastomosis was created using the carotid artery (side) and jugular vein (end) (Fig. 1a). After unclamping, dilation of the vein and patency was confirmed visually. The mice were maintained on a warming blanket following surgery and buprenorphine was administered two times at 12 hours apart. NH was consistently observed by day 21 post-op (Fig. 1b). The control blood vessels were the pre-surgical carotid artery and jugular vein (n = 1), and the contralateral non-surgery carotid artery and jugular vein in the AVF mice at day 7 (n = 1) and day 21 (n = 1) post-operatively.

Surgical procedure and histology: (a) Arteriovenous fistula (AVF) mouse model using jugular vein (end) to carotid artery (side) configuration. Asterisk (*) depicts the arteriovenous anastomosis. The white arrow indicates the direction of blood flow in the venous outflow tract. (b) Representative histology of AVF dysfunction (Movat’s stain). Neointimal hyperplasia (NH) was present at 21 Days

Pike D., Shiu Y.T., Somarathna M., Guo L., Isayeva T., Totenhagen J, & Lee T. (2017). High resolution hemodynamic profiling of murine arteriovenous fistula using magnetic resonance imaging and computational fluid dynamics. Theoretical Biology & Medical Modelling, 14, 5.
Publication 2017
Animals Arteriovenous Anastomosis Blood Circulation Blood Vessel Buprenorphine Common Carotid Artery Fistula, Arteriovenous Hyperplasia Institutional Animal Care and Use Committees Isoflurane Jugular Vein Ketamine Males Mice, House Mice, Inbred C57BL Microscopy Neointima Operative Surgical Procedures Stains Surgical Anastomoses Surgical Wound Sutures Veins Venous Engorgement
5
Volumetric Analysis of Injured Arteries
For OPT imaging, isolated vessels were embedded in 1.5% low melting point agarose (Invitrogen, UK), dehydrated in methanol (100%; 24 hours) and optically cleared in benzyl alcohol:benzyl benzoate (1∶2 v/v; 24 hours). Vessels were imaged using a Bioptonics 3001 OPT tomograph. All studies on injured arteries were performed using emission imaging, after UV illumination (425 nm excitation filter with 40 nm band pass; 475 nm long pass emission filter; 1.048Mpixel scanning resolution). For each vessel type, a magnification was chosen to provide the smallest voxel size whilst allowing the entire region of interest to be covered. This resulted in voxel sizes of 216, 64 and 166 µm3 respectively for wire- and ligation-injured femoral arteries and atherosclerotic aortic arches. For each vessel, exposure time was adjusted to maximise the dynamic range of the resulting image and was approximately 400, 800 and 1000ms per projection for wire- and ligation-injured femoral arteries and atherosclerotic aortic arches, respectively. Raw data (400 projections per scan at 0.9° increments) was subject to Hamming-filtered back-projection using NRecon software (Skyscan, Belgium). Quantification was performed using CTan software (Skyscan, Belgium). Briefly, lesion and lumen volumes were segmented by semi-automated tracing of the internal elastic lamina and subsequent grey-level thresholding to distinguish neointima from lumen. (See Methods S1 for a step-by-step protocol).
Kirkby N.S., Low L., Seckl J.R., Walker B.R., Webb D.J, & Hadoke P.W. (2011). Quantitative 3-Dimensional Imaging of Murine Neointimal and Atherosclerotic Lesions by Optical Projection Tomography. PLoS ONE, 6(2), e16906.
Publication 2011
Arch of the Aorta Arteries Benzyl Alcohol benzyl benzoate Blood Vessel Femoral Artery Internal Elastic Lamella Ligation Methanol Neointima Radionuclide Imaging Sepharose Tomography Ultraviolet Rays

Most recents protocols related to «Neointima»

1
Histopathological Analysis of Vein Grafts
The procedures of histopathological analysis were described in our previous study [49 (link)]. Histological examination of the implanted vein grafts was performed before and 12 weeks after surgery. The harvested samples were fixed in 10% buffered formalin, and cross-sections (5 µm thick) were cut and embedded into paraffin. The morphology of the vessel wall thickness and intimal hyperplasia was analyzed to clarify the layer division using hematoxylin and eosin (H&E) staining. The structure of each histological sections was manually identified using light microscopy (OLYMPUS, BX53). The lumen area, neointimal layer thickness, and medial layer thickness were measured using Image J software version 1.52v (National Institutes of Health, Bethesda, Maryland, USA). The neointima-to-media ratio was assessed. All pathological analysis was performed in a blinded manner.
The collagen density was analyzed using Masson’s trichrome staining. The stains were examined using light microscopy and measurements were made according to the intensity of the color blue, which represented the collagen density. A quantitative analysis was obtained by using the ratio of collagen density in the total area.
Apoptosis was detected by the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method using an in situ apoptosis detection kit (TREVIGEN 4810-30-k). The number of apoptotic cells was counted at 200× magnification in an average of four sections per stained slide using Image J software.
Kim J.H., Jang E.H., Ryu J.Y., Lee J., Kim J.H., Ryu W, & Youn Y.N. (2023). Sirolimus-Embedded Silk Microneedle Wrap to Prevent Neointimal Hyperplasia in Vein Graft Model. International Journal of Molecular Sciences, 24(4), 3306.
Publication 2023
Apoptosis Blood Vessel Collagen deoxyuridine triphosphate DNA Nucleotidylexotransferase Eosin Formalin Grafts Hematoxylin Hyperplasia Light Microscopy Neointima Operative Surgical Procedures Paraffin Embedding Staining Tunica Intima Veins
2
Carotid Artery Injury Model in Mice
Vascular injury was modeled in 10-week-old SM-mtCaMKIIN mice and littermates by endothelial denudation of the left common carotid artery using a resin bead-coated suture. Twenty-eight days after the denudation procedure, all animals were anesthetized. Transcardiac perfusion was performed with 10 ml PBS, after which fixation at physiological pressure was performed by replacing PBS with 10 ml of 4% paraformaldehyde (PFA). The injured left carotid arteries and their contralateral controls were excised, placed in tissue-embedding molds, stored in 4% PFA for 2 days, and embedded in paraffin.
For morphometric analysis of the arteries, 5 μm cross sections were obtained at 50 μm intervals, beginning at the carotid bifurcation site and extending up to 500 μm proximally. Sections were treated with Verhoeff-van Gieson stain and imaged using a Nikon Eclipse TS100 microscope. The areas of the vessel wall, lumen, and intima were determined using tracings of the circumferences of the external elastic lamina (EEL), internal elastic lamina (IEL), and lumen, using NIH Image J. The areas were calculated as follows: that of the vessel wall by subtracting the luminal area from the area defined by the EEL; and that of the neointima by subtracting the luminal area from the area defined by the IEL.
Koval O.M., Nguyen E.K., Mittauer D.J., Ait-Aissa K., Chinchankar W., Qian L., Madesh M., Dai D.F, & Grumbach I.M. (2023). The mitochondrial regulation of smooth muscle cell proliferation in type 2 diabetes. bioRxiv.
Publication Preprint 2023
Animals Arteries Blood Vessel Carotid Arteries Common Carotid Artery Endothelium Fungus, Filamentous Internal Elastic Lamella Mice, Laboratory Microscopy Neointima Paraffin Embedding paraform Perfusion Phenobarbital physiology Pressure Resins, Plant Stains Sutures Tunica Intima Vascular System Injuries
3
Histomorphometric Analysis of Arterial Remodeling
Paraffin-embedded arteries were cut into 5-μm sections and hematoxylin‐eosin (H&E) stained. Lumen area, area inside internal elastic lamina (IEL), area inside external elastic lamina (EEL), and IEL and EEL perimeters were measured on the sections using ImageJ. Calculations were conducted following previous publication [63 (link)]: Neointimal area = IEL area - lumen area; normalized intimal thickness = neointima area/IEL perimeter; stenosis rate = (neointima area/IEL area)*100; neointima/media ratio (I/M ratio) = neointima area/(area inside adventitia – IEL area). The data was generated by averaging 3–4 artery sections from each animal, and then the means from seven animals in each treatment group were averaged to get mean ± SEM (n = 7 rats). The morphometric parameters were measured by a student in a blinded fashion.
Xie X., Shirasu T., Li J., Guo L.W, & Kent K.C. (2023). miR579-3p is an inhibitory modulator of neointimal hyperplasia and transcription factors c-MYB and KLF4. Cell Death Discovery, 9, 73.
Publication 2023
Adventitia Animals Arteries Eosin Hematoxylin Internal Elastic Lamella Neointima Paraffin Perimetry Rattus norvegicus Stenosis Student Tunica Intima
4
Murine Carotid Artery Injury Model
The present study consists of two datasets: One study on the naive carotid artery and one dataset on restenosis. For the analysis of the healthy vessels, cell isolation procedures were partially adapted from Hu et al. (2016) (link) to ensure isolation of bona fide carotid cell populations, rather than cells of the neointima or granulation tissue. When analyzing the injury response of the murine carotid artery, the entire vascular cell population was of interest. Data subset from the latter dataset have been used in a previous study comparing the vascular response in WT and Nox4−/− mice (Buchmann et al., 2021 (link)).
All animal experiments were performed in accordance with the National Institutes of Health Guidelines on the use of laboratory animals. The University Animal Care Committee and the Federal Authorities for Animal Research (Darmstadt, Germany) approved the study protocol (approval number: FU1185). Mice were housed in a specified pathogen-free facility with 12-12 h day and night cycle and free access to water and chow. Wire-induced injury surgery was applied to male mice at an age of 11 weeks. Wire-injury was carried out only on the left carotid of the animal, the right carotid artery served as control (Lindner et al., 1993 (link); Xu, 2004 (link); Bigalke et al., 2011 (link)). Due to the potential for artefacts and variability, any vessels showing signs of thrombosis were removed and not subjected to further analysis.
Warwick T., Buchmann G.K., Pflüger-Müller B., Spaeth M., Schürmann C., Abplanalp W., Tombor L., John D., Weigert A., Leo-Hansmann M., Dimmeler S, & Brandes R.P. (2023). Acute injury to the mouse carotid artery provokes a distinct healing response. Frontiers in Physiology, 14, 1125864.
Publication 2023
Animal Care Committees Animals Animals, Laboratory Blood Cells Blood Vessel Carotid Arteries Carotid Artery Injuries Cells Cell Separation Common Carotid Artery Granulation Tissue Injuries Males Mus Neointima NOX4 protein, human Operative Surgical Procedures Specific Pathogen Free Thrombosis
5
Carotid Artery Neointima Analysis
Carotid tissue samples frozen in OCT compound were prepared as 10 µm serial sections. Sections were stained with Hematoxylin and Eosin staining solutions. The neointima area was analyzed by planimetry with ImageJ.
Warwick T., Buchmann G.K., Pflüger-Müller B., Spaeth M., Schürmann C., Abplanalp W., Tombor L., John D., Weigert A., Leo-Hansmann M., Dimmeler S, & Brandes R.P. (2023). Acute injury to the mouse carotid artery provokes a distinct healing response. Frontiers in Physiology, 14, 1125864.
Publication 2023
Carotid Arteries Eosin Freezing Neointima Tissues

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More about "Neointima"

Neointima, also known as intimal hyperplasia, is a critical pathological process in cardiovascular diseases, characterized by the formation of a new innermost layer within the blood vessel lumen.
This proliferative growth of smooth muscle cells and extracellular matrix can lead to vessel narrowing, known as restenosis, and impaired blood flow, a hallmark of atherosclerosis.
Understanding the mechanisms and factors influencing neointima formation is crucial for developing effective treatments for these cardiovascular conditions.
Researchers can utilize powerful tools like Image-Pro Plus 6.0, a comprehensive image analysis software, to study the cellular and structural changes associated with neointima development.
To optimize their neointima research, scientists can turn to PubCompare.ai, an AI-powered platform that helps locate the best protocols from literature, preprints, and patents.
This data-driven approach not only enhances reproducibility and accuracy but also saves time and effort compared to manual research.
By incorporating synonyms such as 'intimal hyperplasia,' related terms like 'restenosis' and 'atherosclerosis,' and key subtopics including 'smooth muscle cells,' 'extracellular matrix,' and 'vessel narrowing,' researchers can comprehensively explore the intricacies of neointima formation.
Additionally, leveraging software tools like Image-Pro Plus, In Situ Cell Death Detection Kit, Atlantis SR Pro, and Ab28364 (an anti-α-SMA antibody) can provide valuable insights into the cellular and molecular mechanisms underlying this process.
Furthermore, the use of C57BL/6J mice, a widely used animal model in cardiovascular research, and techniques like the Vectostain ABC Elite peroxidase kit and Eclipse 80i microscope can further enhance the understanding of neointima development and its implications for clinical applications.
By embracing this holistic, data-driven approach to neointima research, scientists can unlock new possibilities for advancing our knowledge and developing innovative therapies to address the challenges posed by restenosis, atherosclerosis, and other cardiovascular conditions.