Two 4 mm horizontal OCT scan sets were acquired (each consisting of 100 B-scans), first using the broadband light source (central wavelength 878.4 nm, 186.3 nm bandwidth) and then the standard source (central wavelength 860 nm, 53.7 nm bandwidth). This sequence was repeated a second time, for a total of four scans per eye. Switching between sources took approximately 5 min. Each scan was exported into ImageJ and a single registered average image was generated for each scan set as described above. Visual inspection and then quantitative mapping of the inner limiting membrane (ILM) contour22 (link) was done for each subject to confirm that their broadband and standard-source images were acquired from the same retinal location. It was estimated that the scans between sessions deviated by less than 50 μm in retinal location, which is the distance between two adjacent B scans.
To compare image quality achieved using the standard and broadband light sources, the peak-to-trough ratio and local contrast of five hyper-reflective retinal layers, ILM, external limiting membrane (ELM), inner segment (IS)/outer segment junction (OS) and two retinal pigment epithelium layers, were evaluated for all four averaged images. First, longitudinal reflectivity profiles (LRP)23 (link) were generated at three locations along each averaged scan: the foveal centre, 0.8 mm temporal to the fovea and 0.8 mm nasal to the fovea. Each LRP used for analysis was an average of the five surrounding A-scans at each of the three locations, so these were perpendicular to the total image. From the LRP the local contrast was computed for the above five layers of interest. We define local contrast as a normalised measure of the contrast of a reflective layer, as given byequation 2 .
The signal of the neighbouring troughs for each layer of interest was computed as an average of the neighbouring lower-reflecting layers in the OCT image (using the absolute local minimum). For example, for the ILM, the neighbouring layer trough signal comes from the vitreous and either the outer nuclear layer for the foveal measurement, or the ganglion cell layer for the nasal and temporal measurements. For the ELM, the neighbouring layer trough signal comes from an average of the outer nuclear layer and the inner segment layer. The reported value of local contrast for each layer is an average of the measured contrast for the two averaged images for the broad-band and standard source imaging conditions.
The improvement in resolution was evaluated by comparing the full-width half-height (FWHH) of the ELM layer in the registered and averaged images generated by the standard and broadband light sources. Using ImageJ, five plot profiles, drawn perpendicular through the ELM in the temporal retina, were averaged together. The averaged profiles were fit to a Gaussian function in order to objectively compute FWHH for each of the two light sources. Resolution was computed from the average image created from one broadband scan set and one standard-source scan set for each subject.
To compare image quality achieved using the standard and broadband light sources, the peak-to-trough ratio and local contrast of five hyper-reflective retinal layers, ILM, external limiting membrane (ELM), inner segment (IS)/outer segment junction (OS) and two retinal pigment epithelium layers, were evaluated for all four averaged images. First, longitudinal reflectivity profiles (LRP)23 (link) were generated at three locations along each averaged scan: the foveal centre, 0.8 mm temporal to the fovea and 0.8 mm nasal to the fovea. Each LRP used for analysis was an average of the five surrounding A-scans at each of the three locations, so these were perpendicular to the total image. From the LRP the local contrast was computed for the above five layers of interest. We define local contrast as a normalised measure of the contrast of a reflective layer, as given by
The signal of the neighbouring troughs for each layer of interest was computed as an average of the neighbouring lower-reflecting layers in the OCT image (using the absolute local minimum). For example, for the ILM, the neighbouring layer trough signal comes from the vitreous and either the outer nuclear layer for the foveal measurement, or the ganglion cell layer for the nasal and temporal measurements. For the ELM, the neighbouring layer trough signal comes from an average of the outer nuclear layer and the inner segment layer. The reported value of local contrast for each layer is an average of the measured contrast for the two averaged images for the broad-band and standard source imaging conditions.
The improvement in resolution was evaluated by comparing the full-width half-height (FWHH) of the ELM layer in the registered and averaged images generated by the standard and broadband light sources. Using ImageJ, five plot profiles, drawn perpendicular through the ELM in the temporal retina, were averaged together. The averaged profiles were fit to a Gaussian function in order to objectively compute FWHH for each of the two light sources. Resolution was computed from the average image created from one broadband scan set and one standard-source scan set for each subject.