Consecutive orthodontic records of various time periods within a 12-year period (2006–2018, depending on the place of sample collection) were searched for eligible subjects at the following clinics: (a) University of Bern, Switzerland (b) University of Athens, Greece, (c) two private practices in Athens and two in Thessaloniki, Greece, and (d) one private practice in Biel, Switzerland. Sample collection was performed at the place of data generation by colleagues who were blinded to the aim of this study.
The sample was collected based on the following inclusion criteria:
• Individuals with an age between 12.5 and 40 years
• Individuals with and without agenesis of teeth other than third molars for the agenesis and the control group, respectively
• European ancestry
• No syndromes, systemic diseases or other defects that affect the craniofacial complex development, as reported in the subjects’ medical records
• Adequate quality panoramic radiographs for identification of missing teeth (
Fig. S1)
• No individuals where the cause of missing teeth was unclear
• No individuals where the presence or absence of teeth could not be confirmed
The minimum age limit of 12.5 years was determined according to previous studies that evaluated the correlation between chronological age and the degree of third molar mineralization (Caldas et al., 2011 (
link); De Oliveira et al., 2012 (
link); Karataş et al., 2013 (
link); Soares et al., 2015 (
link); Zandi et al., 2015 (
link)). They showed that in 95% of cases, Demirjian’s stage A was observed at the age of 12.5 or younger, which means that the mineralization of third molar crowns has already started and is clearly visible on the panoramic radiographs.
Finally, the panoramic radiographs of 303 individuals with agenesis of teeth other than third molars (agenesis group) were selected from a large orthodontic sample of approximately 10,000 individuals, based on availability. A control group of 303 individuals without agenesis of teeth other than third molars, matched for age (within 6 months) and sex was formed from the same archives. All other inclusion criteria for the control group, were the same as mentioned above for the agenesis group.
Scheiwiller M., Oeschger E.S, & Gkantidis N. (2020). Third molar agenesis in modern humans with and without agenesis of other teeth. PeerJ, 8, e10367.