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Macrostomia

Macrostomia is a rare congenital condition characterized by an abnormally wide mouth opening.
It can occur as an isolated anomaly or as part of a larger syndrome.
Macrostomia may lead to difficulties with feeding, speech, and facial aesthetics.
Effective management often requires a multidisciplinary approach involving specialists in plastic surgery, dentistry, and speech therapy.
Reseachers can leverage AI-driven tools like PubCompare.ai to optimize their Macrostomia research by identifiying the most effective protocols and products from the literature, preprints, and patents.
This can enhance the reproducibilty and accelerate progress in this importnat area of study.

Most cited protocols related to «Macrostomia»

The Helgoland mud area (Figure 1) in the German Bight of the North Sea extends over ∼500 km2 and has a water depth of less than 30 m (Hebbeln et al., 2003 (link)). It represents one of the few depocenters of fine-grained sediments in the North Sea. The average sedimentation rate was estimated to have been high (13 mm yr-1) between 750–1550 before present. Presently, it is at 1.6 mm yr-1 (Hebbeln et al., 2003 (link)). During RV HEINCKE cruise HE376 in April 2012, subsurface sediment samples were collected from site HE 376-007 (Table 1) using a gravity corer (GC; 5 m core length). The upper 20–30 cm of sediment is generally lost when using a GC. Therefore, a multi corer (MUC) was used to collect undisturbed surface sediments (30 cm core length) from the same site during cruises with RV UTHÖRN in September 2012 (UT-2012; Table 1) and with RV HEINCKE in April 2014 (HE421-004; Table 1). The 5 m-long gravity core HE376-007-5 was cut in 25 cm sections, and subsamples of each section, taken with sterile 10 ml cut-off syringes, were frozen at -80°C for molecular analyses. For the MUC core UT-2012, only the top 10 cm of the 30 cm-long sediment core was processed for molecular analysis as this corresponded to the depth at which high dissolved iron was measured based on previous geochemical investigations at this study site. The sediment samples from the top 10 cm were homogenized and stored in separate 50 ml Falcon tubes at -80°C until further use. For pore-water and solid-phase analyses, a parallel gravity core at site HE376-007 was taken and sampled. Pore-water was retrieved every 25 cm by means of rhizon samplers which have an average pore size of 0.15 μm according to procedures described by Seeberg-Elverfeldt et al. (2005) (link) and Dickens et al. (2007) (link). For the measurement of methane concentrations, 5 ml of wet sediment were taken with cut-off syringes and were inserted into 50 ml headspace vials pre-filled with 20 ml of saturated NaCl solution. The vials were tightly closed with rubber septa, sealed with aluminum crimps and stored inverted at +4°C to minimize methane loss.
For Mössbauer spectroscopy, GC, and MUC cores collected during RV HEINCKE cruises HE406 and HE421 conducted in July 2013 and April 2014, respectively, were used (Table 1). In the home lab, the GC retrieved from site HE406-008 was cut into 25 cm sections and the top 10 cm of the MUC core (UT-2012) were sampled as described above. 10 g of wet sediment were transferred into wide-mouth glass vials under a stream of argon gas (99.998% purity, Linde, Germany), frozen at -20°C and then freeze-dried to avoid air oxygen contamination. The freeze-drier chamber was filled with argon, lyophilized samples were removed immediately, sealed with rubber septa, and headspaces were flushed with argon on a manifold.
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Publication 2015
A-007 Aluminum Argon Freezing Gravity Iron Macrostomia Methane Oxygen Rubber Sodium Chloride Spectroscopy, Mossbauer Sterility, Reproductive Syringes
The phantom was constructed from standard labware components: a 50ml polypropylene conical tube (30mm × 115mm) and 1000ml polypropylene wide-mouth jar. The 50ml tube was filled with distilled water, capped and cemented to the underside of the 1000ml jar top. Prior to diffusion measurements, cubed or crushed ice and water were added such that ice filled the full extent of the jar. By screwing on the jar top, the 50ml tube of water was held in the center ice water mixture. The phantom was wrapped in a hospital “blue pad” for insulation and to absorb surface condensate. Figure 1 illustrates the phantom (Figure 1A), its suggested positioning for scanning and a representative T1-weighted image through the center of the phantom (Figure 1B).
Separate from the MRI experiment, a thermocouple was inserted into the conical tube for determination of the time required to reach thermal equilibration between the water in the conical tube and the surrounding ice bath starting from room temperature..
Publication 2011
ARID1A protein, human Bath Diffusion Macrostomia Polypropylenes
Semen samples were obtained from 27 males following 2 days of sexual abstinence. The specimens were taken by masturbation into a sterile wide mouth container. The samples were liquefied at 37 °C for 30 min. All experiments were performed within 1 h after sampling. Only ejaculates showing normal semen parameters (concentration > 20 × 106/mL, motility > 40%, viability > 40%, and morphology > 4%) and free from leukocytes were used. The experiments were approved by the Ethic Committee at the Specialized Hospital Sv. Svodar Zobor, protocol no. 030809/2015. Tryptone Soya agar (TSA, Merck, Darmstadt, Germany) and Blood agar (BA, Merck, Darmstadt, Germany) were inoculated with the semen samples, and after incubation (24 h, 37 °C), individual colonies were selected for further confirmation with MALDI-TOF MS Biotyper (Brucker Daltonics, Bremen, Germany) [78 (link)]. The isolates were maintained in Mueller Hinton Agar (MHA, Merck, Darmstadt, Germany) and cultured 24 h before the experiment to reach a concentration of 105 cfu/mL.
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Publication 2020
Agar BLOOD Ethics Committees Leukocytes Macrostomia Males Motility, Cell Plant Embryos Soybeans Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sterility, Reproductive
Sequence-verified plasmids were transformed into NEB5a strain E. coli (New England Biolabs) (because the promoter in the pNCST vector is semi-constitutive in most strains of E. coli, we find it convenient to use a single strain for cloning and expression), plated on LB/agar supplemented with carbenicillin (100 μg/ml), and incubated overnight at 37°C. For proteins that matured efficiently at 37°C (AvicFP-F64L, mAvicFP1, AvicFP4, AausFP1, AausFP2, EGFP, mEGFP, and mNeonGreen), colonies were picked and inoculated directly into a 200-ml baffled wide-mouth flask containing 50 ml of 2xYT broth and 100 μg/ml carbenicillin, and incubated overnight at 37°C with shaking at 250 rpm. For proteins requiring multiple days at room temperature to mature (avGFP, AvicFP1, AvicFP2, AvicFP3, AausFP3, and AausFP4), a single colony was resuspended in 10 ml of 2xYT medium, and 100 μl of this suspension was plated on 5 100-mm petri dishes containing LB/agar and 100 μg/ml carbenicillin. After overnight incubation at 37°C to initially establish colonies, plates were then incubated at room temperature for several days in the dark.
Bacteria containing the recombinant protein were recovered by centrifuging liquid cultures in 50-ml conical tubes at 4,500g for 10 minutes. For proteins expressed on LB/agar plates, a razor blade was gently glided over the surface of the agar, harvesting the colonies on the blade, and then wiped into 2-ml microcentrifuge tubes and gently centrifuged to the bottom of the tube. Four milliliters of the lysis reagent B-PER (Thermo 78248) was added for every gram of E. coli pellet. Tubes were gently vortexed until the pellets were completely dissolved, taking care not to form bubbles from the detergent component of the B-PER. The resulting suspension was then incubated on a gentle rocker for 15 minutes and then centrifuged at >20,000g for 10 minutes to pellet insoluble debris. Note that we find that there is a strong correlation between true protein solubility and extraction efficiency in B-PER that is not true of other extraction methods such as sonication, which can solubilize aggregated FPs more readily.
Meanwhile, we prepared a purification column by adding 1–2 ml of Ni-NTA resin slurry (Expedeon) into a 15-ml gravity column (Bio-Rad), allowing the storage buffer to drip through. The column was equilibrated with 10 bed volumes of wash buffer (150 mM Tris [pH 7.5], 300 mM NaCl, 5 mM imidazole) and then capped at the bottom. After centrifugation, the lysate was directly added to the prepared Ni-NTA column. The column was then capped at the top and the lysate-resin slurry was tumbled end-over-end for 30 minutes at 4°C. The top/bottom caps were removed, and the liquid was allowed to drip through by gravity flow. The column was then washed 3 times with 3 column volumes of wash buffer. Finally, the protein was eluted from the column by gradual addition of elution buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 200 mM imidazole). Clear liquid was allowed to drip through, and only the fluorescent/colorful fraction was collected.
The proteins were then concentrated further using a 3-kD MWCO column (Amicon/Millipore) until the volume of protein solution was <150 μl. Meanwhile, 2× desalting columns (Pierce) were prepared for each protein by equilibrating in 50 mM Tris (pH 8.5)/150 mM NaCl according to the manufacturer’s instructions. Then 150 μl of protein solution was loaded onto the equilibrated desalting column and centrifuged at 1,500 rpm for 1 minute in a microcentrifuge. The collected protein was then passed through a second equilibrated desalting column to ensure complete buffer exchange.
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Publication 2020
Agar Bacteria Buffers Carbenicillin Centrifugation Cloning Vectors Detergents Escherichia coli Gravity Hyperostosis, Diffuse Idiopathic Skeletal imidazole Macrostomia Pellets, Drug Plasmids Proteins Recombinant Proteins Resins, Plant Sodium Chloride Staphylococcal Protein A Strains Tromethamine
Samples were collected during cruise ANT-28/5 (10 April – 15 May 2012 with RV Polarstern) at 26 stations across a latitudinal transect in the Atlantic Ocean (51 °S–47 °N). At all stations, samples were consistently collected from five depths of the epipelagic zone: 20 m, 40 m, 60 m, 100 m and 200 m. Seven samples were taken ±10 m from the designated depths (Table S2). Sampling was carried out with 12 L Niskin bottles mounted on a CTD probe (Sea-Bird Electronics Inc. SBE 911 plus probe) equipped with double temperature and conductivity sensors, a pressure sensor, altimeter, chlorophyll fluorometer and transmissometer. CTD data were validated during the cruise through regular reference measurements of water samples applying standard methods. Immediately after retrieval on deck, 12 L of sample water from one bottle was transferred to 20 L wide-mouth barrels, and filtered with three peristaltic pumps (Ismatec, IDEX Health & Science GmbH, Wertheim Germany) through three successive stainless steel filtration devices (Druckfiltrationsgerät Edelstahl Typ 1627, Omnilab Laborzentrum, Braunschweig, Germany) equipped with the following membrane filters (diameter 142 mm): 8 μm (mixed cellulose ester SCWP14250, Millipore, Darmstadt, Germany), 3 μm (mixed cellulose ester SSWP14250, Millipore, Darmstadt, Germany), and 0.22 μm (polyethersulfone GPWP14250, Millipore, Darmstadt, Germany). After filtration membranes were immediately stored at −80 °C until DNA isolation. The three communities were named: free-living (FL) for the 0.22 μm membranes, small particle associated (SPA) for the 3 μm membranes and large particle associated (LPA) for the 8 μm membranes (Supplementary Table S2).
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Publication 2016
Aves Cellulose Chlorophyll Electric Conductivity Esters Filtration isolation Macrostomia Medical Devices Peristalsis polyether sulfone Pressure Stainless Steel Tissue, Membrane

Most recents protocols related to «Macrostomia»

Add 40 grams of each carbon source into a 1,000 mL wide mouth bottle, and mixed with 800 ml natural seawater (salinity 31 ± 1 ‰, pH 7.72 ± 0.15, DO 5.56 ± 0.41, COD 2.32 ± 1.17). The bottle was then sealed with a plastic cap to prevent evaporation and contamination. The experimental temperature was 25°C and the experimental period was 30 days. The water in the bottle was collected every 2 days for determination of COD, TOC, and, DOC. Analysis of CODs was done according to the method described in the Standard Method for Examination of Water and Wastewater GB 17378.7-1998 (1999) . TOC and DOC were measured using a TOC analyzer (Shimadzu, TOC-L) with an automatic sampler (Shimadzu, ASI-V). At the end of this experiment, the contents of short-chain fatty acids (SCFAs) in water were measured. SCFAs, including acetic acid (AC), propionic acid (PA), butyric acid (BA), and other types (isobutyric acid, valeric acid, isovaleric acid, and hexanoic acid) were measured by ion chromatography-mass spectrometry (Shimadzu GCMS QP2010-ULTRA, Japan). After each sampling, an equal amount of sterilized seawater was added to the mouth-wide mouth bottle to keep the volume constant. Before and after the experiment, the carbon source samples were analyzed by Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. The carbon sources were crushed and randomly sampled to observed using SEM (ZEISS Gemini SEM 300, Germany), and the carbon distribution on the surface of the solid carbon source was analyzed using SEM-energy dispersive X-ray spectroscopy (EDS) (ZEISS Gemini SEM 300, Germany). The functional group information of carbon sources was identified by FTIR using a FTIR spectrometer (Thermo Scientific Nicolet iS20, USA). The physical properties of the three repeated tests are shown in Table 1.
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Publication 2023
Acetic Acid Butyric Acids Carbon Chromatography Cods Energy Dispersive X Ray Spectroscopy Fatty Acids, Volatile Gas Chromatography-Mass Spectrometry hexanoic acid isobutyric acid isovaleric acid Macrostomia Mass Spectrometry Physical Processes propionic acid Salinity Scanning Electron Microscopy Spectroscopy, Fourier Transform Infrared valeric acid
This study referenced the design of past experimental surveys21 (link). To assess smoking behavior, this survey inquired “Do you currently have a habit of smoking?” with options comprising “currently smoking”, “already quit smoking”, and “never smoked”. Those indicating that they have quit smoking or had never smoked were considered to have no smoking behavior. Second-hand smoke exposure was assessed by asking research participants “Has anyone smoked in front of you within the last 30 days?” with options comprising “yes” and “no”.
Urine samples were collected on the same day as the questionnaire surveys. The urine of each research participant was collected in 500 mL wide-mouth bottle containers, and then placed in – 4 °C ice buckets for storage. Subsequently, the samples were transported to a laboratory within 4 hours for storage in a refrigerator at – 20 °C. Within one week of collection, urine cotinine concentration analysis was conducted with enzyme-linked immunosorbent assays (ELISA) kits (Calbiotech Co., Spring Valley, CA, USA), with participants who exhibited urine cotinine concentrations above 50 ng/mL considered to be smokers and who exhibited urine cotinine concentrations under 50 ng/mL considered to be nonsmokers (Society for Research on Nicotine and Tobacco Subcommittee on Biochemical Verification, 2002). The testing process can be found in our previous study20 (link).
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Publication 2023
Cotinine Enzyme-Linked Immunosorbent Assay Macrostomia Nicotiana tabacum Nicotine Non-Smokers Urinalysis Urine

Obtain and process field samples. We manually collected approximately 6.5 L of a surficial portion (top several centimeters) benthic biomat from the field site using 1 L wide mouth Nalgene polypropylene bottles. The harvested biomat was stored and transported at 4 °C. This step will vary according to the system, but the goal is to obtain a representative sample that maintains viability.

Thoroughly clean the flow-through reactors. We perform a dilute bleach soak, a water rinse, and 70% ethanol wipe down followed by air drying.

Mark the interior of the flow-through reactors with 25 mm vertical increments using an indelible marker, or deploy rulers to monitor biomat depth.

With clean, autoclaved utensils and a large mixing bowl, homogenize the biomat or sediment from the field sample and add to the flow-through reactors with a target depth of approximately 1 cm (this is potentially an experiment-specific variable).

Add influent media to desired depth and let the benthic biomat settle for approximately 12 h with the grow lights off. The settling time limits the sediment from flowing out of the reactors when influent flow begins.

Start the desired flow of influent representative of field waters, or a medium modified for experimental needs. We use a flow rate that creates a hydraulic residence time in the open water column of 12 – 48 h, depending on the experimental goals. Examples of flow rate and media calculations are included in the supplementary information.

While the depth of biomat and open water can be adjusted to experimental needs, we recommend adding at least 1 cm of biomat material to capture the diverse microbial consortia living both above and below the biomat oxic-anoxic interface, which is inferred to be within the surficial 1 cm of the biomat-water interface [13] (link). For the purposes of method validation with readily available data, biomat from the Prado Constructed Wetlands (Orange County, California) was used for seeding of the flow-through reactors. The depth of the water column was 3.5 cm and the depth of the biomat was 2 cm. The diel cycling of pH and DO is typically observed within 3 days of flow start-up, with steady-state diel cycling observed in 5–7 days.
Especially for experiments where the fate and transport of nutrients or trace inorganics are of interest, a parallel biomat-free reactor aids in hypothesis testing. We have observed nitrogen transformation in biomat-free systems, presumably due to adsorption to materials such as pump tubing, or microbial contamination within flow lines or influent containers (data not shown). Material compatibility can also be an issue, as we identified trace aluminum leaching from raw aluminum flow-through reactors that complex with trace metalloids in water (data not shown). Laboratory environmental variables such as humidity and elevation, and instrument accuracy and precision are easier to understand by including a biomat-free control reactor operated in parallel to the experimental ones.
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Publication 2023
Adsorption Aluminum Anoxia Ethanol Humidity Light Macrostomia Metalloids Microbial Consortia Nitrogen Nutrients Polypropylenes Technique, Dilution Wetlands
College campuses across Massachusetts underwent the transition to
remote learning in March 2020. As a result, classrooms, residence
halls, and assembly buildings (e.g., dining services) were closed
and non-essential research operations were halted on multiple campuses,
including Northeastern University (NEU) in Boston. On June 1, NEU
partially reopened for research activities at established laboratory
capacity limits of 25% for the remainder of the year. For the fall
2020 semester, residence halls and classrooms opened at reduced capacity
and NEU operated with hybrid learning, which included part in-person
and part remote learning. In this study, three NEU buildings (hereafter,
commercial buildings) and four residential households were included.
These site types (commercial buildings and residential households)
(i) had ranges of size, age, and functionality, (ii) were situated
within 5 miles of each other, and (iii) were served with chloraminated
water from the same DWDS. Cold water taps at each site type, i.e.,
commercial building (three sites, two taps per site) and residential
household (four sites, one tap per site), were sampled during the
first week of each month for 6 months starting the month of building
reopening (i.e., June 2020). The sampled taps were in residential
kitchens (n = 2), commercial kitchenettes (n = 3), residential bathrooms (n = 2),
and research laboratories in commercial buildings (n = 3). Flush profiles were conducted after overnight stagnation at
all 10 taps between 6:00 and 10:00 a.m. on two consecutive days (Table S1). Seven 10 mL samples were collected
from each tap in sterile 15 mL polyethylene centrifuge tubes (Falcon,
catalog no. 352196) for flow cytometric analyses; this included the
first draw sample following overnight stagnation [time point 1 (TP0, 0 min)] and six samples collected at 5 min intervals over
a flush period of 30 min (TP5, 5 min; TP10,
10 min; TP15, 15 min; TP20, 20 min; TP25, 25 min; and TP30, 30 min). An additional 2 L sample
was collected at TP0 and TP30 in sterile narrow-mouth
polycarbonate bottles (Thermo Scientific, catalog no. DS22050210)
and used for DNA-based microbial community characterization, as well
as 500 mL samples in wide-mouth HDPE bottles (Thermo Scientific, catalog
no. 02-896-2E) for chemical analysis. Temperature measurements were
obtained at 10 s intervals using an Elitech GSP-6 data logger during
the flushing period and flow rates averaged at 4.10 ± 1.80 L
min–1 (Table S1).
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Publication 2023
Cold Temperature Flow Cytometry Households Hybrids Macrostomia Microbial Community Polyethylene, High-Density Sterility, Reproductive
Four groups of artificial substrates were placed side-by-side in the central area of each experimental interval and marked. We collected several equal areas of benthic algae and mixed them into one sample for measurement to reduce experimental errors. A polyvinyl chloride tube with an outer diameter of approximately 3.7 cm was placed in each group of artificial substrates. The benthic algae in the area were scraped with a toothbrush and rinsed with distilled water into a wide-mouth plastic bottle as a quantitative sample of benthic algae. The remaining benthic algae were scraped into another wide-mouth plastic bottle, transported back to the laboratory, and added to Lugol’s solution and 4% formaldehyde solution for fixed preservation as qualitative samples of benthic algae.
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Publication 2023
Biologic Preservation Formalin Lugol's solution Macrostomia Polyvinyl Chloride Toothbrushing

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More about "Macrostomia"

Macrostomia is a rare congenital condition characterized by an abnormally wide mouth opening.
This condition, also known as large mouth or wide mouth, can occur as an isolated anomaly or as part of a larger syndrome.
Individuals with macrostomia may experience difficulties with feeding, speech, and facial aesthetics.
Effective management often requires a multidisciplinary approach, involving specialists in plastic surgery, dentistry, and speech therapy.
Researchers studying macrostomia can leverage AI-driven tools like PubCompare.ai to optimize their research by identifying the most effective protocols and products from the literature, preprints, and patents.
This can enhance the reproducibility and accelerate progress in this important area of study.
Related terms and subtopics include congenital malformations, craniofacial abnormalities, feeding difficulties, speech impairment, facial aesthetics, plastic surgery, dentistry, speech therapy, PubCompare.ai, AI-driven tools, literature review, preprints, patents, and research optimization.
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