For Mössbauer spectroscopy, GC, and MUC cores collected during RV HEINCKE cruises HE406 and HE421 conducted in July 2013 and April 2014, respectively, were used (
Macrostomia
It can occur as an isolated anomaly or as part of a larger syndrome.
Macrostomia may lead to difficulties with feeding, speech, and facial aesthetics.
Effective management often requires a multidisciplinary approach involving specialists in plastic surgery, dentistry, and speech therapy.
Reseachers can leverage AI-driven tools like PubCompare.ai to optimize their Macrostomia research by identifiying the most effective protocols and products from the literature, preprints, and patents.
This can enhance the reproducibilty and accelerate progress in this importnat area of study.
Most cited protocols related to «Macrostomia»
For Mössbauer spectroscopy, GC, and MUC cores collected during RV HEINCKE cruises HE406 and HE421 conducted in July 2013 and April 2014, respectively, were used (
Bacteria containing the recombinant protein were recovered by centrifuging liquid cultures in 50-ml conical tubes at 4,500g for 10 minutes. For proteins expressed on LB/agar plates, a razor blade was gently glided over the surface of the agar, harvesting the colonies on the blade, and then wiped into 2-ml microcentrifuge tubes and gently centrifuged to the bottom of the tube. Four milliliters of the lysis reagent B-PER (Thermo 78248) was added for every gram of E. coli pellet. Tubes were gently vortexed until the pellets were completely dissolved, taking care not to form bubbles from the detergent component of the B-PER. The resulting suspension was then incubated on a gentle rocker for 15 minutes and then centrifuged at >20,000g for 10 minutes to pellet insoluble debris. Note that we find that there is a strong correlation between true protein solubility and extraction efficiency in B-PER that is not true of other extraction methods such as sonication, which can solubilize aggregated FPs more readily.
Meanwhile, we prepared a purification column by adding 1–2 ml of Ni-NTA resin slurry (Expedeon) into a 15-ml gravity column (Bio-Rad), allowing the storage buffer to drip through. The column was equilibrated with 10 bed volumes of wash buffer (150 mM Tris [pH 7.5], 300 mM NaCl, 5 mM imidazole) and then capped at the bottom. After centrifugation, the lysate was directly added to the prepared Ni-NTA column. The column was then capped at the top and the lysate-resin slurry was tumbled end-over-end for 30 minutes at 4°C. The top/bottom caps were removed, and the liquid was allowed to drip through by gravity flow. The column was then washed 3 times with 3 column volumes of wash buffer. Finally, the protein was eluted from the column by gradual addition of elution buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 200 mM imidazole). Clear liquid was allowed to drip through, and only the fluorescent/colorful fraction was collected.
The proteins were then concentrated further using a 3-kD MWCO column (Amicon/Millipore) until the volume of protein solution was <150 μl. Meanwhile, 2× desalting columns (Pierce) were prepared for each protein by equilibrating in 50 mM Tris (pH 8.5)/150 mM NaCl according to the manufacturer’s instructions. Then 150 μl of protein solution was loaded onto the equilibrated desalting column and centrifuged at 1,500 rpm for 1 minute in a microcentrifuge. The collected protein was then passed through a second equilibrated desalting column to ensure complete buffer exchange.
Most recents protocols related to «Macrostomia»
Urine samples were collected on the same day as the questionnaire surveys. The urine of each research participant was collected in 500 mL wide-mouth bottle containers, and then placed in – 4 °C ice buckets for storage. Subsequently, the samples were transported to a laboratory within 4 hours for storage in a refrigerator at – 20 °C. Within one week of collection, urine cotinine concentration analysis was conducted with enzyme-linked immunosorbent assays (ELISA) kits (Calbiotech Co., Spring Valley, CA, USA), with participants who exhibited urine cotinine concentrations above 50 ng/mL considered to be smokers and who exhibited urine cotinine concentrations under 50 ng/mL considered to be nonsmokers (Society for Research on Nicotine and Tobacco Subcommittee on Biochemical Verification, 2002). The testing process can be found in our previous study20 (link).
Obtain and process field samples. We manually collected approximately 6.5 L of a surficial portion (top several centimeters) benthic biomat from the field site using 1 L wide mouth Nalgene polypropylene bottles. The harvested biomat was stored and transported at 4 °C. This step will vary according to the system, but the goal is to obtain a representative sample that maintains viability.
Thoroughly clean the flow-through reactors. We perform a dilute bleach soak, a water rinse, and 70% ethanol wipe down followed by air drying.
Mark the interior of the flow-through reactors with 25 mm vertical increments using an indelible marker, or deploy rulers to monitor biomat depth.
With clean, autoclaved utensils and a large mixing bowl, homogenize the biomat or sediment from the field sample and add to the flow-through reactors with a target depth of approximately 1 cm (this is potentially an experiment-specific variable).
Add influent media to desired depth and let the benthic biomat settle for approximately 12 h with the grow lights off. The settling time limits the sediment from flowing out of the reactors when influent flow begins.
Start the desired flow of influent representative of field waters, or a medium modified for experimental needs. We use a flow rate that creates a hydraulic residence time in the open water column of 12 – 48 h, depending on the experimental goals. Examples of flow rate and media calculations are included in the supplementary information.
Especially for experiments where the fate and transport of nutrients or trace inorganics are of interest, a parallel biomat-free reactor aids in hypothesis testing. We have observed nitrogen transformation in biomat-free systems, presumably due to adsorption to materials such as pump tubing, or microbial contamination within flow lines or influent containers (data not shown). Material compatibility can also be an issue, as we identified trace aluminum leaching from raw aluminum flow-through reactors that complex with trace metalloids in water (data not shown). Laboratory environmental variables such as humidity and elevation, and instrument accuracy and precision are easier to understand by including a biomat-free control reactor operated in parallel to the experimental ones.
remote learning in March 2020. As a result, classrooms, residence
halls, and assembly buildings (e.g., dining services) were closed
and non-essential research operations were halted on multiple campuses,
including Northeastern University (NEU) in Boston. On June 1, NEU
partially reopened for research activities at established laboratory
capacity limits of 25% for the remainder of the year. For the fall
2020 semester, residence halls and classrooms opened at reduced capacity
and NEU operated with hybrid learning, which included part in-person
and part remote learning. In this study, three NEU buildings (hereafter,
commercial buildings) and four residential households were included.
These site types (commercial buildings and residential households)
(i) had ranges of size, age, and functionality, (ii) were situated
within 5 miles of each other, and (iii) were served with chloraminated
water from the same DWDS. Cold water taps at each site type, i.e.,
commercial building (three sites, two taps per site) and residential
household (four sites, one tap per site), were sampled during the
first week of each month for 6 months starting the month of building
reopening (i.e., June 2020). The sampled taps were in residential
kitchens (n = 2), commercial kitchenettes (n = 3), residential bathrooms (n = 2),
and research laboratories in commercial buildings (n = 3). Flush profiles were conducted after overnight stagnation at
all 10 taps between 6:00 and 10:00 a.m. on two consecutive days (
from each tap in sterile 15 mL polyethylene centrifuge tubes (Falcon,
catalog no. 352196) for flow cytometric analyses; this included the
first draw sample following overnight stagnation [time point 1 (TP0, 0 min)] and six samples collected at 5 min intervals over
a flush period of 30 min (TP5, 5 min; TP10,
10 min; TP15, 15 min; TP20, 20 min; TP25, 25 min; and TP30, 30 min). An additional 2 L sample
was collected at TP0 and TP30 in sterile narrow-mouth
polycarbonate bottles (Thermo Scientific, catalog no. DS22050210)
and used for DNA-based microbial community characterization, as well
as 500 mL samples in wide-mouth HDPE bottles (Thermo Scientific, catalog
no. 02-896-2E) for chemical analysis. Temperature measurements were
obtained at 10 s intervals using an Elitech GSP-6 data logger during
the flushing period and flow rates averaged at 4.10 ± 1.80 L
min–1 (
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More about "Macrostomia"
This condition, also known as large mouth or wide mouth, can occur as an isolated anomaly or as part of a larger syndrome.
Individuals with macrostomia may experience difficulties with feeding, speech, and facial aesthetics.
Effective management often requires a multidisciplinary approach, involving specialists in plastic surgery, dentistry, and speech therapy.
Researchers studying macrostomia can leverage AI-driven tools like PubCompare.ai to optimize their research by identifying the most effective protocols and products from the literature, preprints, and patents.
This can enhance the reproducibility and accelerate progress in this important area of study.
Related terms and subtopics include congenital malformations, craniofacial abnormalities, feeding difficulties, speech impairment, facial aesthetics, plastic surgery, dentistry, speech therapy, PubCompare.ai, AI-driven tools, literature review, preprints, patents, and research optimization.
Researchers may also find the QIAamp DNA Stool Mini Kit, NanoDrop ND-1000, PAL-10S, Wide-mouth water sampling bottles, S-Pak Filters, 7500 series instrument, M-TEC agar, SCA CASA System, Wide-Mouth Straight-Sided PMP Jars, and Clean wide-mouth packers useful in their macrostomia-related studies, depending on the specific experimental needs.