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> Disorders > Disease or Syndrome > Atherosclerosis

Atherosclerosis

Atherosclerosis is a progressive disease characterized by the accumulation of lipids, cholesterol, and other substances in and on the walls of the arteries.
This buildup, known as plaque, can narrow the arteries and restrict blood flow, potentially leading to serious complications such as heart attack, stroke, or limb amputation.
PubCompare.ai leverages AI to optimize research protocols, locate best procedures from literature, preprints, and patents, and provide AI-driven comparisons for enhanced reproducibility and accuracy.
This streamlining of the research workflow can unlock new insights into the complexity of atherosclerosis, ultimately advancing our understanding and treatment of this widespread cardiovascular condition.

Most cited protocols related to «Atherosclerosis»

1
Metabolic Profiling and Microbiome Modulation in Atherosclerosis
Plasma samples and associated clinical study data were identified in patients referred for cardiac evaluation at a tertiary care center. All subjects gave written informed consent and the Institutional Review Board of the Cleveland Clinic approved all study protocols. Unbiased metabolic profiling was performed using liquid chromatography coupled to electrospray ionization mass spectrometry (LC/MS). Target analyte structural identification was achieved using a combination of LC/MS/MS, LC-MSn, multinuclear NMR, gas chromatography-mass spectrometry, and choline isotope tracer feeding studies in mice as outlined in Methods. Statstical analyses were performed using R (version 2.10.1)36 . Intestinal microflora was suppressed by supplementation of drinking water with a cocktail of broad spectrum antibiotics37 (link). Germ-free mice were purchased from Taconic SWGF. QTL analyses to identify atherosclerosis related genes were performed on F2 mice generated by crossing atherosclerosis prone C57BL/6J.apoe−/− mice and atherosclerosis resistant C3H/HeJ.apoe−/− mice38 (link). mRNA expression was assayed by Microarray Analysis and Real Time PCR. Aortic root lesion area in mice was quantified by microscopy after staining39 (link). Mouse peritoneal macrophages were collected by lavage for foam cell quantification and cholesterol accumulation assay. Surface protein levels of scavenger receptors, CD36, SR-A1, were determined by flow cytometry.
Wang Z., Klipfell E., Bennett B.J., Koeth R., Levison B.S., DuGar B., Feldstein A.E., Britt E.B., Fu X., Chung Y.M., Wu Y., Schauer P., Smith J.D., Allayee H., Tang W.H., DiDonato J.A., Lusis A.J, & Hazen S.L. (2011). Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease. Nature, 472(7341), 57-63.
Publication 2011
Aortic Root ApoE protein, human Atherosclerosis Biological Assay Cholesterol Choline Ethics Committees, Research Flow Cytometry Foam Cells Gas Chromatography-Mass Spectrometry Genes Heart Intestinal Microbiome Liquid Chromatography Macrophages, Peritoneal Mice, Inbred C57BL Microarray Analysis Microscopy Mus NMR, Multinuclear Patients Plasma Radionuclide Imaging Real-Time Polymerase Chain Reaction RNA, Messenger Scavenger Receptor Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry
2
MASALA Study Eligibility Criteria
To be eligible for the MASALA study participants had to have (1) South Asian ancestry defined by having at least 3 grandparents born in one of the following countries: India, Pakistan, Bangladesh, Nepal, or Sri Lanka; and (2) age between 40 and 79 years; (3) ability to speak and/or read English, Hindi or Urdu. A pilot study called the Metabolic syndrome and Atherosclerosis in South Asians Living in America (NIH grant #K23 HL080026) had similar eligibility criteria and methods as this larger study. The 150 participants enrolled in the pilot study were eligible to enroll in the current MASALA study.
We used identical exclusion criteria to MESA12 (link) which included having a physician diagnosed heart attack, stroke or transient ischemic attack, heart failure, angina, use of nitroglycerin, or those with a history of cardiovascular procedures such as coronary artery bypass graft surgery, angioplasty, valve replacement, pacemaker or defibrillator implantation, or any surgery on the heart or arteries. Those with current atrial fibrillation or in active treatment for cancer were excluded. Those with life expectancy < 5 years due to a serious medical illness, impaired cognitive ability as judged by the reviewer, plans to move out of the study region in the next 5 years, or those living in a nursing home or on a waiting list were also excluded. Due to CT scanner limitations, those weighing over 300 lbs. were excluded.
Kanaya A.M., Kandula N., Herrington D., Budoff M.J., Hulley S., Vittinghoff E, & Liu K. (2013). Mediators of Atherosclerosis in South Asians Living in America (MASALA) Study: Objectives, Methods, and Cohort Description. Clinical Cardiology, 36(12), 713-720.
Publication 2013
Angina Pectoris Angioplasty Arteries Asian Persons Atherosclerosis Atrial Fibrillation Cardiovascular System CAT SCANNERS X RAY Cerebrovascular Accident Childbirth Cognition Congestive Heart Failure Coronary Artery Bypass Surgery Defibrillators Eligibility Determination Grandparent Infantile Neuroaxonal Dystrophy Malignant Neoplasms Metabolic Syndrome X Myocardial Infarction Nitroglycerin Ovum Implantation Pacemaker, Artificial Cardiac Physicians South Asian People Surgical Procedure, Cardiac Transient Ischemic Attack
3
Socioeconomic Status, Race, and Diurnal Cortisol

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Publication 2010
1-palmitoyl-2-oleoylphosphatidylethanolamine Atherosclerosis Biological Assay BLOOD Cardiovascular Diseases Disease Progression Emotions Epistropheus Ethnicity Family Member Fingers Gender Gossypium Head Health Planning Heart Hispanics Hormones Hostility Households Hydrocortisone Hypersensitivity Index, Body Mass Luminescent Measurements Lung Medical Devices Metabolic Equivalent Obesity Parent Saliva Sjogren's Syndrome Specimen Collection Steroids Trapezoid Bones Viscosity Woman
4
Prospective Study of Stable CAD Patients
MIPS is a prospective study of stable CAD patients enrolled between June 2011 and August 2014 from Emory University affiliated hospitals and clinics, including Emory University Hospital, Grady Memorial Hospital, Emory Midtown Hospital and the Atlanta VA Medical Center. Patients were enrolled if they were 30 to 79 years of age and had documented CAD defined as any of the following: an abnormal coronary angiogram demonstrating evidence of atherosclerosis with at least luminal irregularities, previous percutaneous or surgical coronary revascularization, a history of myocardial infarction (MI), or a positive nuclear stress test. Patients were excluded if they had an acute coronary syndrome or decompensated heart failure in the prior week, severe psychiatric conditions other than major depression, pregnancy (women of childbearing age were screened by pregnancy test), uncontrolled high blood pressure (≥180/110 mmHg), or with contraindications for regadenoson administration. Beta-adrenergic antagonists were held for 24 hours and calcium channel blockers and nitrates for at least 12 hours prior to the stress test. Patients for whom withholding medications was considered unsafe were excluded. The diagnosis of heart failure was made using the following criteria: a) self-reported history of heart failure that was confirmed by chart review; b) medical chart review for previous diagnosis of heart failure (all patients were seen by a cardiologist at Emory affiliated hospitals before enrollment); c) ICD codes and adjudication by research personnel. Coronary angiographic data were collected by chart review with a median time between the angiogram and enrollment of 2.1 (1.0 – 4.4) years. The Emory University Institutional Review Board approved the research protocol, and all participants provided written informed consent.
Hammadah M., Al Mheid I., Wilmot K., Ramadan R., Shah A.J., Sun Y., Pearce B., Garcia E.V., Kutner M., Bremner J.D., Esteves F., Raggi P., Sheps D.S., Vaccarino V, & Quyyumi A.A. (2017). The Mental Stress Ischemia Prognosis Study (MIPS): Objectives, Study Design, and Prevalence of Inducible Ischemia. Psychosomatic medicine, 79(3), 311-317.
Publication 2016
Acute Coronary Syndrome Adrenergic beta-Antagonists Angiography ARID1A protein, human Atherosclerosis Calcium Channel Blockers Cardiologists Congestive Heart Failure Coronary Angiography Diagnosis Disorder, Depressive Ethics Committees, Research Exercise Tests Heart High Blood Pressures Myocardial Infarction Nitrates Operative Surgical Procedures Patients Pharmaceutical Preparations Phenobarbital Pregnancy Pregnancy Tests regadenoson TNPO1 protein, human Vision Woman
5
Genomic Epidemiology of Cardiovascular Traits
Data from 62,266 participants from the following eleven studies in the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium [3] (link) were included in this joint calling experiment and study descriptions were published previously: Age, Gene/Environment, Susceptibility-Reykjavik (AGES) Study [8] (link), Atherosclerosis Risk in Communities (ARIC) Study [9] (link), Cardiac Arrest Blood Study (CABS) [10] (link), Cardiovascular Health Study (CHS) [11] (link), [12] (link), Coronary Artery Risk Development in Young Adults (CARDIA) [13] (link), [14] (link), Multi-Ethnic Study of Atherosclerosis (MESA) [15] (link), Family Heart Study (FamHS) [16] (link), Framingham Heart Study (FHS) [17] (link), Health, Aging, and Body Composition (HABC) Study [18] (link), Jackson Heart Study (JHS) [19] , and the Rotterdam Study (RS) [20] –[23] (link). In addition, we genotyped 96 unrelated HapMap samples (48 CEU and 48 YRI) with each cohort and the list of sample IDs are available as a reference on the CHARGE exome chip public website.
Grove M.L., Yu B., Cochran B.J., Haritunians T., Bis J.C., Taylor K.D., Hansen M., Borecki I.B., Cupples L.A., Fornage M., Gudnason V., Harris T.B., Kathiresan S., Kraaij R., Launer L.J., Levy D., Liu Y., Mosley T., Peloso G.M., Psaty B.M., Rich S.S., Rivadeneira F., Siscovick D.S., Smith A.V., Uitterlinden A., van Duijn C.M., Wilson J.G., O’Donnell C.J., Rotter J.I, & Boerwinkle E. (2013). Best Practices and Joint Calling of the HumanExome BeadChip: The CHARGE Consortium. PLoS ONE, 8(7), e68095.
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Publication 2013
Artery, Coronary Atherosclerosis BLOOD Body Composition Cardiac Arrest Cardiovascular System DNA Chips Exome Genes Genome HapMap Heart Joints Susceptibility, Disease Young Adult

Most recents protocols related to «Atherosclerosis»

1
Cyclic Peptides Reduce Atherosclerosis
Not available on PMC !

Example 5

Methods.

Experiments were performed in male apoE−/− mice fed an atherogenic diet (D12108, cholate-free AIN-76A semi-purified diet, Research Diets Inc., New Brunswick, NJ) from 4 weeks of age. MPE-298 (300 nmol/kg), MPE-267 (300 nmol/kg) or vehicle (0.9% NaCl), were administered by daily by subcutaneous (s.c.) injections for 8 weeks, from 12 weeks of age, as shown schematically in FIG. 8A.

Results and Discussion.

The results depicted in FIG. 8B show that chronic treatment of the animals with cyclic peptides MPE-267 and MPE-298 reduced aortic lesions by 28% and 42% (p<0.01), respectively, relative to 0.9% NaCl (vehicle), providing evidence that the cyclic peptides exhibit anti-atherosclerotic activity.

US11879021B2. Cyclic peptides and uses thereof (2024-01-23). UNIVERSITÉ DE MONTRÉAL [CA]. Inventors: William D. Lubell [CA], Huy Ong [CA], Jinqiang Zhang [CN], Dilan Mukandila Mulumba [CA], Sylvie Marleau [CA], Ragnhild Gaard Ohm [DK], Ahsanullah [CA], Samy Omri [CA], Ramesh Chingle [US].
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Patent 2024
Animals Aorta Apolipoproteins E Atherosclerosis Cholate Cyclic Peptides Diet Males Mus Normal Saline
2
Comprehensive Stroke Risk Assessment
In this study, clinical data were collected from the enrolled patients, including demographics (age and sex); vascular risk factors (hypertension, diabetes mellitus, and ischemic heart disease); baseline blood pressure [systolic blood pressure (SBP) and diastolic blood pressure (DBP)]; Trial of Org 10 172 in Acute Stroke Treatment (TOAST) [large-artery atherosclerosis, cardioembolism, small-vessel occlusion, acute stroke of other determined etiology, stroke of undetermined etiology]; stroke severity (SS) [defined as mild stroke according to the National Institutes of Health Stroke Scale (NIHSS) scores of ≤ 8, moderate-to-severe stroke according to NIHSS scores of ≥9; all assessments completed on admission]; magnetic resonance imaging (MRI) features [stroke distribution (SD; anterior circulation, posterior circulation, and anterior/posterior circulation), side of hemisphere (SOH; left, right, and bilateral), number of stroke lesions (NOSs; single and multiple stroke lesions), site of stroke lesions (SOSs; cortical, cortico-subcortical, subcortical, brainstem, and cerebellum)]; laboratory tests [total cholesterol, triglycerides, low-density lipoprotein (LDL), fasting blood glucose (FBG), homocysteine (HCY), uric acid (UA), fibrinogen (FIB), myoglobin (MB), C-reactive protein (CRP), D-dimer brain natriuretic peptide (BNP), HBALC, neuron-specific enolase (NSE), and S-100β levels], treatment regimen [intravenous thrombolysis, arterial thrombolysis, antiplatelet, anticoagulation, statin, and proton pump inhibitor therapy (PPI)]; and stroke comorbidities [dysphagia and stroke-associated pneumonia (SAP)].
Wang K., Gu L., Liu W., Xu C., Yin C., Liu H., Rong L., Li W, & Wei X. (2023). The predictors of death within 1 year in acute ischemic stroke patients based on machine learning. Frontiers in Neurology, 14, 1092534.
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Publication 2023
Acute Cerebrovascular Accidents Arteries Atherosclerosis Blood Glucose Blood Pressure Blood Vessel Brain Stem Cerebellum Cerebrovascular Accident Cholesterol Cortex, Cerebral C Reactive Protein Deglutition Disorders Dental Occlusion Diabetes Mellitus fibrin fragment D Fibrinogen Fibrinolytic Agents gamma-Enolase High Blood Pressures Homocysteine Hydroxymethylglutaryl-CoA Reductase Inhibitors Low-Density Lipoproteins Myocardial Ischemia Myoglobin Nesiritide Patients Pneumonia Pressure, Diastolic Proton Pump Inhibitors Systolic Pressure Therapeutics Treatment Protocols Triglycerides Uric Acid
3
Atherosclerosis Progression in ApoE-/- Mice
Our mice strain came from the Nanjing Model Animal Resource Information Platform. Apoe−/− male C57BL/6 mice (16 weeks old) were used to establish control and experimental group. APOE is often produced in monocytes and macrophages (Curtiss et al., 2000 (link)) and plays a critical role in blood lipid metabolism (Chen et al., 2017 (link)) as ligands for receptors that clear chylomicron and VLDL residue (Meir and Leitersdorf, 2004 (link)). So when APOE is knocked out, total cholesterol in plasma increases (Maganto-Garcia, Tarrio, and Lichtman, 2012 (link)), and the effect is multiplied especially under a high-fat and high-cholesterol diet. Female mice secrete estrogen, which lowers the content of LDL in plasma and enhances endovascular blood coagulation (Aryan et al., 2020 (link)). For the experimental group, to accelerate the progression of atherosclerosis, the mice were fed with high-fat and high-cholesterol food for about 12 weeks after they had been weaned (4 weeks old); this group is referred to as the Western diet (HFD) group for short. (Formula of high fat, high cholesterol diet: 20% sucrose, 15% lard, 1.2% cholesterol, 0.2% sodium cholate, 10% casein, 0.6% calcium hydrogen phosphate, 0.4% stone powder, 0.4% premix and 52.2% basal feed.) Meanwhile, another group of mice, the control group, was administered with a chow diet. Mice were euthanized after 12 weeks of being administered different diets. Animal studies were performed in compliance with ethical guidelines and use of animals, and the experimental protocol was approved by the Shenzhen University Animal Care and Use Committee.
Wen J., Ling R., Chen R., Zhang S., Dai Y., Zhang T., Guo F., Wang Q., Wang G, & Jiang Y. (2023). Diversity of arterial cell and phenotypic heterogeneity induced by high-fat and high-cholesterol diet. Frontiers in Cell and Developmental Biology, 11, 971091.
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Publication 2023
Animal Model Animals ApoE protein, human Atherosclerosis BLOOD Calculi Caseins Cholesterol Chylomicrons Coagulation, Blood dicalcium phosphate Diet Diet, High-Fat Disease Progression Estrogens Females Food Hypercholesterolemia lard Ligands Lipid A Macrophage Males Metabolism Mice, House Mice, Inbred C57BL Monocytes Plasma Powder Sodium Cholate Strains Sucrose Therapy, Diet
4
Atherosclerosis Biomarker Profiling
A total of 156 volunteers were recruited for this study, of which 80 were patients with atherosclerosis and 76 were healthy people matching the age and sex of the atherosclerosis group. The inclusion criteria for the atherosclerosis group included: patients diagnosed with carotid atherosclerosis by carotid artery ultrasonography. Exclusion criteria included rheumatic heart disease, recent traumatic surgical history, liver and kidney insufficiency, tumor, and hemorrhagic disease. After all volunteers were enrolled in the group, 5 mL of fasting venous blood was collected in anticoagulation tube, and the serum was separated after centrifugation and stored in −80 °C refrigerator for later use.
This research protocol has been approved by the Ethics Committee of Taihe Hospital, Hubei University of Medicine and follows the guidelines of the Declaration of Helsinki on human experiments. All volunteers have signed written informed consent.
Chen J., Tang Z., Chen Z., Wei Y., Liang H., Zhang X., Gao Z, & Zhu H. (2023). MicroRNA-218-5p regulates inflammation response via targeting TLR4 in atherosclerosis. BMC Cardiovascular Disorders, 23, 122.
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Publication 2023
Atherosclerosis BLOOD Carotid Atherosclerosis Centrifugation Ethics Committees, Clinical Hemorrhagic Disorders Homo sapiens Liver Neoplasms Operative Surgical Procedures Patients Pharmaceutical Preparations Renal Insufficiency Rheumatic Heart Disease Serum Ultrasonography, Carotid Arteries Veins Voluntary Workers
5
Evaluating miR-218-5p in Atherosclerosis
SPSS 22.0 software was used for data analysis in this study. According to the normality of the data (using Kolmogorov–Smirnov test), parametric t-test or non-parametric Mann–Whitney U test was performed. Receiver operating characteristic (ROC) curve was used to evaluate the clinical diagnostic accuracy of abnormally expressed miR-218-5p in atherosclerosis. The value of Pearson correlation coefficient analysis is to evaluate the correlation between the level of miR-218-5p and the index. Data are presented as mean ± standard deviation [15 (link)]. P < 0.05 was defined as significantly different.
Chen J., Tang Z., Chen Z., Wei Y., Liang H., Zhang X., Gao Z, & Zhu H. (2023). MicroRNA-218-5p regulates inflammation response via targeting TLR4 in atherosclerosis. BMC Cardiovascular Disorders, 23, 122.
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Publication 2023
Atherosclerosis Diagnosis

Top products related to «Atherosclerosis»

1
Td 88137 by Inotiv
Sourced in United States, United Kingdom, Montenegro
The TD.88137 is a laboratory equipment product from Inotiv. It is designed for use in scientific research and analysis applications. The core function of the TD.88137 is to perform a specific task or measurement, but a detailed description is not available while maintaining an unbiased and factual approach.
2
Oil red o by Merck Group
Sourced in United States, Germany, China, Japan, United Kingdom, Sao Tome and Principe, Italy, Macao, Australia, France, Switzerland, Spain, India, Poland, Canada
Oil Red O is a fat-soluble dye used in histology and cell biology for the staining of neutral lipids, such as triglycerides and cholesterol esters. It is a useful tool for the identification and visualization of lipid-rich structures in cells and tissues.
3
Apoe mice by Jackson ImmunoResearch
Sourced in United States, Montenegro
ApoE−/− mice are genetically modified mice that lack the apolipoprotein E (ApoE) gene. This gene plays a crucial role in lipid metabolism and transport. ApoE−/− mice are commonly used as an animal model in research to study the development and progression of atherosclerosis, a condition characterized by the buildup of fatty deposits in the arteries.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
C57bl 6j by Jackson ImmunoResearch
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C57BL/6J is a mouse strain commonly used in biomedical research. It is a common inbred mouse strain that has been extensively characterized.
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Image pro plus 6 by Media Cybernetics
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Image-Pro Plus 6.0 is a comprehensive image analysis software package designed for scientific and industrial applications. It provides a wide range of tools for image capture, enhancement, measurement, analysis, and reporting.
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Image pro plus software by Media Cybernetics
Sourced in United States, Japan, Germany, United Kingdom, Canada, China, Denmark
Image-Pro Plus is an image analysis software developed by Media Cybernetics. It provides tools for image acquisition, processing, measurement, and analysis.
8
Apoe mice by Charles River Laboratories
Sourced in China, Germany, United States, Italy
ApoE−/− mice are genetically engineered mice that lack the apolipoprotein E (ApoE) gene. This gene plays a critical role in cholesterol and lipid metabolism. ApoE−/− mice are commonly used in research to study atherosclerosis, lipid disorders, and cardiovascular disease.
9
C57bl 6 mice by Jackson ImmunoResearch
Sourced in United States, Montenegro, Canada, China, France, United Kingdom, Japan, Germany
C57BL/6 mice are a widely used inbred mouse strain commonly used in biomedical research. They are known for their black coat color and are a popular model organism due to their well-characterized genetic and physiological traits.
10
Prism 8 by GraphPad
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Prism 8 is a data analysis and graphing software developed by GraphPad. It is designed for researchers to visualize, analyze, and present scientific data.

More about "Atherosclerosis"

Atherosclerosis, a progressive cardiovascular disease, is characterized by the accumulation of lipids, cholesterol, and other substances in and on the walls of arteries.
This buildup, known as plaque, can narrow the arteries and restrict blood flow, potentially leading to serious complications such as heart attack, stroke, or limb amputation.
PubCompare.ai, an innovative AI-driven platform, revolutionizes the research landscape by streamlining the workflow and optimizing protocols for studying atherosclerosis.
The platform leverages artificial intelligence to locate the best procedures from literature, preprints, and patents, providing AI-driven comparisons for enhanced reproducibility and accuracy.
This approach unlocks new insights into the complexity of atherosclerosis, a widespread condition affecting the cardiovascular system.
Researchers can utilize tools like TD.88137, Oil Red O staining, and ApoE−/− mouse models to delve deeper into the pathophysiology of the disease.
Additionally, the use of cell culture techniques, such as those involving fetal bovine serum (FBS) and C57BL/6J mouse-derived cells, can further elucidate the underlying mechanisms.
Advanced imaging software, such as Image-Pro Plus 6.0 and Prism 8, enable detailed analysis and visualization of atherosclerotic lesions in preclinical studies.
By harnessing the power of AI and integrating various research methodologies, PubCompare.ai empowers researchers to uncover new avenues for understanding and treating atherosclerosis, ultimately advancing the field of cardiovascular medicine.