Details of breeding and husbandry, including preparation of encapsulated (enteric-released) rapamycin, have been described at length in earlier papers (Miller et al. 2007 (link); Miller et al. 2011 (link); Strong et al. 2013 (link)), as have results of both end-of-life (Miller et al. 2011 (link)) and midlife (Wilkinson et al. 2012 (link)) necropsy analyses. In brief, genetically heterogeneous UM-HET3 mice were produced by a cross between CByB6F1/J mothers (JAX #100009) and C3D2F1/J fathers (JAX #100004), at each of three test sites: the Jackson Laboratory (TJL), the University of Michigan (UM), or the University of Texas Health Science Center at San Antonio (UT). They were housed at 3 male mice or 4 female mice per cage from weaning, and at nine months of age were given a diet containing encapsulated rapamycin at 4.7, 14, or 42 ppm (mg of drug per kg of food). Mice that died were not replaced, so cage density declined at older ages. Cages were inspected daily. Date of death was noted for mice found dead, and mice found to be so ill that they were expected to die within the next 24 – 48 hr were euthanized, with the date of euthanasia taken as the date of death for life table calculations. At the time of analysis, 532 control mice had died, and 2 control mice (0.4%) were alive; 749 mice exposed to rapamycin had died, and 15 (1.9%) were alive. In addition, 5 mice were removed prior to natural death because of experimental accidents (example: death during implantation of ID tag); 6 were removed for humane reasons because of dermatitis; 74 males were removed according to our protocol policy which requires euthanasia of all mice in cages in which any mouse is found to have extensive fight wounds (Miller et al. 2007 (link)); and 104 mice, all at TJL, were removed for use in a separate study of immune function.
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Dermatitis
Dermatitis
Dermatitis is a general term that refers to inflammation of the skin.
It can have various causes, including allergic reactions, irritants, and underlying medical conditions.
Symptoms may include redness, itching, blistering, and scaling.
Dermatitis can affect any area of the body, but it is commonly seen on the hands, face, and other exposed skin areas.
Proper diagnosis and management, which may involve avoidance of triggers, topical treatments, and sometimes systemic medications, are important for controlling symptoms and preventing progression.
Reasearchers can leverage PubCompare.ai to optimize dermatitis studies by easily comparing data from literature, preprints, and patents to enhance reproducibility and accuracy in this complex field.
It can have various causes, including allergic reactions, irritants, and underlying medical conditions.
Symptoms may include redness, itching, blistering, and scaling.
Dermatitis can affect any area of the body, but it is commonly seen on the hands, face, and other exposed skin areas.
Proper diagnosis and management, which may involve avoidance of triggers, topical treatments, and sometimes systemic medications, are important for controlling symptoms and preventing progression.
Reasearchers can leverage PubCompare.ai to optimize dermatitis studies by easily comparing data from literature, preprints, and patents to enhance reproducibility and accuracy in this complex field.
Most cited protocols related to «Dermatitis»
Accidents
Autopsy
Dermatitis
Diet
Euthanasia
Fathers
Females
Food
Genetic Heterogeneity
Immune System Processes
Males
Mice, House
Mothers
Ovum Implantation
Pharmaceutical Preparations
Sirolimus
Wounds
Content validation was conducted through qualitative analysis of in‐depth one‐to‐one patient interviews to test the relevance, wording, applicability, usability, recall period and response options of the Peak Pruritus NRS in patients with moderate‐to‐severe AD. Interview participants had to be at least 18 years of age, have physician‐reported diagnosis of AD, have had AD for at least 3 years, have had moderate‐to‐severe itching related to AD in the past month, and not have participated in a related clinical trial or focus group in the past 6 months. Moderate‐to‐severe itching related to AD in the past was measured by response to the survey questions (i) ‘At its worst, would you describe the dermatitis‐related itching as mild, moderate, severe or extremely severe?’ (participants must answer ‘moderate’, ‘severe’ or ‘extremely severe’ to qualify) and (ii) ‘When was your last itching episode, as related to atopic dermatitis: in the past 2 weeks, past month, past 2 months or more than 2 months ago?’ (participants must answer ‘in the past 2 weeks’ or ‘in the past month’ to qualify).
We aimed to recruit a sample of patients with diversity of sex, race, ethnicity and level of education. Interviews included concept elicitation about AD symptoms and cognitive debriefing regarding the Peak Pruritus NRS. All interviews were conducted in English by the same pair of skilled, experienced interviewers following a semistructured interview guide, and verbatim responses were transcribed. Patients provided signed written informed consent, and all study materials were reviewed and approved by the institutional review board of RTI International (Raleigh, NC, U.S.A.).
We aimed to recruit a sample of patients with diversity of sex, race, ethnicity and level of education. Interviews included concept elicitation about AD symptoms and cognitive debriefing regarding the Peak Pruritus NRS. All interviews were conducted in English by the same pair of skilled, experienced interviewers following a semistructured interview guide, and verbatim responses were transcribed. Patients provided signed written informed consent, and all study materials were reviewed and approved by the institutional review board of RTI International (Raleigh, NC, U.S.A.).
Cognition
Dermatitis
Diagnosis
Eczema
Ethics Committees, Research
Ethnicity
Interviewers
Mental Recall
Patients
Physicians
Pruritus
Generation of Il22ra2−/− mice, tissue and cell isolation, flow cytometry, quantitative RT-PCR, generation of human immunoglobulin G Fc fusion protein, in vitro CD4+ T-cell differentiation assay, cell culture, skin inflammation, histopathological assessment, and immunohistochemical analysis are described in Supplementary Material.
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Biological Assay
CD4 Positive T Lymphocytes
Cell Culture Techniques
Cell Separation
Dermatitis
Flow Cytometry
Immunoglobulin G
Mus
NR4A2 protein, human
Reverse Transcriptase Polymerase Chain Reaction
Tissues
Adult
Allergists
Asthma
Biopsy
Blood Vessel
Dermatitis
Endoscopy
Endoscopy, Gastrointestinal
Eosinophil
Eosinophilia
Esophageal Diseases
Esophagitis
Esophagus
Ethics Committees, Research
Exudate
Food
Food Allergy
Gender
Hiatal Hernia
Hypersensitivity
Inflammation
Mucous Membrane
Pathological Dilatation
Pathologists
Patients
Phenotype
Proton Pump Inhibitors
Retreatments
Rhinitis, Allergic
Senile Plaques
Sinusitis
Stenosis
Steroids
For cell transplantation studies, 16-month-old male C57BL/6 mice were obtained from the National Institute on Aging (NIA). Mice were housed 4-5 per cage. Mice were sorted using body weight from low to high. Next, either SEN or CON transplant treatments were assigned to every other mouse using a random number generator, with the intervening mice being assigned to the other treatment, so that pairs of SEN- and CON-transplanted mice were matched by weight. After 1 month of acclimation, cells were transplanted at age 17 months. Physical function tests were performed 1 month after transplantation, at age 18 months. After that, no further tests were performed on these mice except for checking their cages. The earliest death occurred approximately 2 months after the last physical function test. For D+Q studies, 19-21-month-old C57BL/6 mice were obtained from the NIA. Mice were housed 3-5 per cage. As with the transplanted mice, animals were sorted based body weight and randomly assigned to D+Q or V treatment by a person unaware of the study design. Starting at age 24-27 months, mice were treated every 2 weeks with D+Q or V by oral gavage for 3 consecutive days. Some of the mice were moved from their original cages during the course of the study to minimize single cage-housing stress. RotaRod and hanging tests were conducted monthly because these tests are sensitive and non-invasive. We euthanized mice and scored them as having died if they exhibited more than one of the following signs59 : 1) unable to drink or eat; 2) reluctant to move even with stimulus; 3) rapid weight loss; 4) severe balance disorder; or 5) bleeding or ulcerated tumor. No mouse was lost due to fighting, accidental death, or dermatitis. The Cox proportional hazard model was used for survival analyses.
Accidents
Acclimatization
Animals
Body Weight
Cells
Cell Transplantation
Dermatitis
Males
Mice, Inbred C57BL
Mus
Neoplasms
Physical Examination
Transplantation
Tube Feeding
Most recents protocols related to «Dermatitis»
The study will be carried out in 16 primary care health centres in the Region of Madrid.
It will include participants aged 18 or older, with a diagnosis of venous ulcer recorded in the electronic medical record (ABI greater than 0.8 and less than 1.3; diameter of the lesion greater than or equal to 1 cm) and under treatment in primary care nursing consultations. The individuals must be able to walk with or without the aid of devices, understand and answer the questionnaires autonomously, be accessible throughout the duration of the study and have expressed their agreement to participate and signed the consent form.
Those who are unable to commute to the health centre, or who reside outside the area where the research is carried out for more than 6 months per year, will be excluded. People with mixed ulcers, deep vein thrombosis (DVT) in acute phase, decompensated heart failure, dermatitis in acute phase, rheumatoid arthritis, undergoing treatment with antineoplastic drugs or with some absolute contraindication for physical exercise will also be excluded.
Withdrawal criteria are set for patients who, during the course of the trial, present a change in their clinical condition that prevents them from further participation, such as inflammation of the locomotor system (with heat, flushing, pain and functional impotence) or trauma due to a fall during the course of program with or without fracture and/or haematoma at both joint and soft tissue level (muscle and tendons) [24 ], must drop out of the study.
It will include participants aged 18 or older, with a diagnosis of venous ulcer recorded in the electronic medical record (ABI greater than 0.8 and less than 1.3; diameter of the lesion greater than or equal to 1 cm) and under treatment in primary care nursing consultations. The individuals must be able to walk with or without the aid of devices, understand and answer the questionnaires autonomously, be accessible throughout the duration of the study and have expressed their agreement to participate and signed the consent form.
Those who are unable to commute to the health centre, or who reside outside the area where the research is carried out for more than 6 months per year, will be excluded. People with mixed ulcers, deep vein thrombosis (DVT) in acute phase, decompensated heart failure, dermatitis in acute phase, rheumatoid arthritis, undergoing treatment with antineoplastic drugs or with some absolute contraindication for physical exercise will also be excluded.
Withdrawal criteria are set for patients who, during the course of the trial, present a change in their clinical condition that prevents them from further participation, such as inflammation of the locomotor system (with heat, flushing, pain and functional impotence) or trauma due to a fall during the course of program with or without fracture and/or haematoma at both joint and soft tissue level (muscle and tendons) [24 ], must drop out of the study.
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Antineoplastic Agents
Congestive Heart Failure
Deep Vein Thrombosis
Dermatitis
Diagnosis
Erectile Dysfunction
Fracture, Bone
Hematoma
Inflammation
Joints
Medical Devices
Muscle Tissue
Musculoskeletal System
Pain
Patients
Primary Health Care
Rheumatoid Arthritis
Tendons
Tissues
Ulcer
Varicose Ulcer
Wounds and Injuries
This study included 83,048 prisoners. Most were male (89.59%), and 10.41% were women. We measured the outcomes based on the ICD-9 codes (680–709). Among them, ICD9_680-686 represents infections of the skin and subcutaneous tissue; ICD9_690-698 represents other inflammatory conditions of the skin and subcutaneous tissue; ICD9_700-709 represents other diseases of the skin and subcutaneous tissue. To maintain strict algorithms, only patients diagnosed at least three times in one group were treated as disease cases [15 (link)].
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Dermatitis
Males
Patients
Prisoners
Skin Diseases
Skin Diseases, Infectious
Subcutaneous Fat
Woman
In trial 3, in each section of houses, randomly selected broiler feet (n = 10/section and four sections/house) were evaluated with a scoring range of 0–2. Footpad data were analyzed in a pass and fail manner. Lesion scorings on the feet of birds were given as follows: 0—no evidence of footpad dermatitis (pass), 1—minimal evidence of footpad dermatitis (fail), and 2—evidence of footpad dermatitis (fail). Pass birds have normal color and minimal swelling, and no lesion was found in more than half the area of the central pad. Fail birds have discoloration and swelling, and lesions were found in more than half the area of the central pad. Footpad condition was checked at 28 and 42 days of age, and the percentage of failed birds to the inspected birds was obtained and compared among treatment groups.
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Aves
Dermatitis
Foot
For the psoriasis mouse model, the dorsal skin of mice was shaved 2 days prior to the application of the topical treatment. Mice were initially anesthetised with 4% isoflurane until movement arrest was observed, then maintained with a continuous flow of 1% isoflurane in an isoflurane chamber. IMQ cream (5%) (Mingxin Pharmaceuticals, Sichuan, China), 62.5 mg, was topically applied to the shaved 2 cm × 3 cm skin portion for six consecutive days. Control mice were treated with 62.5 mg of Vaseline (Vas) as vehicle control under the same conditions. The first day of IMQ or Vas application was defined as Day 0, and mice were euthanised on Day 4 and Day 6.
For the exogenous IL-33 administration, 1 μg of recombinant murine IL-33 (PeproTech, USA, cat: 210–33) or phosphate-buffered saline (PBS; control) in a final volume of 100 μL were administered daily through intraperitoneal injection, 1 h prior to IMQ treatment.
The severity of skin inflammation was evaluated daily by two independent researchers, using the scoring system of the psoriasis severity index (PSI). This score rated erythema, scaling, and skin thickness on a scale from 0 to 4: 0—none; 1—slight; 2—moderate; 3—marked; and 4—very marked, according to previous studies [16 (link)]. A cumulative score was generated from these parameters (scale 0–12). Mice were euthanised and samples harvested for further analyses at the respective time points.
For the exogenous IL-33 administration, 1 μg of recombinant murine IL-33 (PeproTech, USA, cat: 210–33) or phosphate-buffered saline (PBS; control) in a final volume of 100 μL were administered daily through intraperitoneal injection, 1 h prior to IMQ treatment.
The severity of skin inflammation was evaluated daily by two independent researchers, using the scoring system of the psoriasis severity index (PSI). This score rated erythema, scaling, and skin thickness on a scale from 0 to 4: 0—none; 1—slight; 2—moderate; 3—marked; and 4—very marked, according to previous studies [16 (link)]. A cumulative score was generated from these parameters (scale 0–12). Mice were euthanised and samples harvested for further analyses at the respective time points.
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Administration, Topical
Animal Scales
CM 2-3
Dermatitis
Erythema
IL33 protein, human
Injections, Intraperitoneal
Isoflurane
Movement
Mus
Pharmaceutical Preparations
Phosphates
Psoriasis
Saline Solution
Skin
Vaseline
Protocol full text hidden due to copyright restrictions
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Abscess
Arm, Upper
Bacteremia
Cannulation
Catheters
Dental Occlusion
Dermatitis
Erythema
Infection
Phlebitis
Sepsis
Thrombophlebitis
Top products related to «Dermatitis»
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The Micrometer is a precision measuring instrument used to measure the thickness or diameter of small objects with high accuracy. It consists of a U-shaped frame with a spindle that rotates a thimble-shaped sleeve, allowing for precise measurements down to 0.01 millimeters or 0.001 inches.
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IMQ cream is a laboratory product manufactured by 3M. It is a sterile, white, water-miscible cream formulation.
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The Digital Caliper is a precision measurement tool designed to accurately measure distances, depths, and thicknesses. It features a digital display that provides highly accurate readings, typically with a resolution of 0.01mm or 0.0005 inches. The caliper is constructed with durable materials and is easy to use, making it a versatile tool for a wide range of applications in various industries.
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RNAlater is a RNA stabilization solution developed by Thermo Fisher Scientific. It is designed to protect RNA from degradation during sample collection, storage, and transportation. RNAlater stabilizes the RNA in tissues and cells, allowing for efficient RNA extraction and analysis.
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SAS 9.4 is an integrated software suite for advanced analytics, data management, and business intelligence. It provides a comprehensive platform for data analysis, modeling, and reporting. SAS 9.4 offers a wide range of capabilities, including data manipulation, statistical analysis, predictive modeling, and visual data exploration.
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C57BL/6J mice are a widely used inbred mouse strain. They are a commonly used model organism in biomedical research.
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Aldara is a laboratory equipment product manufactured by 3M. It is designed for use in research and scientific applications. The core function of Aldara is to provide a controlled environment for various laboratory procedures and experiments.
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An electronic balance is a precise weighing instrument used to determine the mass or weight of an object. It utilizes electronic sensors to measure and display the weight value digitally. The core function of an electronic balance is to provide accurate and reliable weight measurements for various applications in laboratories, research facilities, and manufacturing environments.
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The MC903 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but its core function is to provide precise temperature control and monitoring capabilities. The MC903 can be used to maintain specific temperatures for a variety of applications, such as incubation, sample preparation, and other temperature-sensitive processes. No further details or interpretations on the intended use of this product are provided.
More about "Dermatitis"
Dermatitis is a broad term encompassing various inflammatory skin conditions, including eczema, contact dermatitis, and atopic dermatitis.
These skin disorders can be triggered by a range of factors, such as allergic reactions, irritants, and underlying medical problems.
Symptoms may include redness, itching, blistering, and scaling, often affecting the hands, face, and other exposed areas.
Proper diagnosis and management are crucial for controlling symptoms and preventing progression.
This may involve identifying and avoiding triggers, utilizing topical treatments like Micrometer, IMQ cream, and Aldara, and in some cases, administering systemic medications.
Researchers can leverage powerful tools like PubCompare.ai to optimize their dermatitis studies by easily comparing data from literature, preprints, and patents, enhancing reproducibility and accuracy in this complex field.
Digital calipers, RNAlater, SAS 9.4, and C57BL/6J mice are some of the tools and resources that can aid in dermatitis research.
By harnessing the power of data comparison and AI-driven insights, researchers can unlock new possibilities for advancing our understanding and treatment of these skin conditions.
These skin disorders can be triggered by a range of factors, such as allergic reactions, irritants, and underlying medical problems.
Symptoms may include redness, itching, blistering, and scaling, often affecting the hands, face, and other exposed areas.
Proper diagnosis and management are crucial for controlling symptoms and preventing progression.
This may involve identifying and avoiding triggers, utilizing topical treatments like Micrometer, IMQ cream, and Aldara, and in some cases, administering systemic medications.
Researchers can leverage powerful tools like PubCompare.ai to optimize their dermatitis studies by easily comparing data from literature, preprints, and patents, enhancing reproducibility and accuracy in this complex field.
Digital calipers, RNAlater, SAS 9.4, and C57BL/6J mice are some of the tools and resources that can aid in dermatitis research.
By harnessing the power of data comparison and AI-driven insights, researchers can unlock new possibilities for advancing our understanding and treatment of these skin conditions.