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Soft Tissue Infection

Soft Tissue Infections are a group of diverse conditions affecting the skin, muscle, and connective tissues.
These infections can range from minor skin abrasions to severe, life-threatening necrotizing fasciitis.
Prompt diagnosis and appropriate treatment are crucial to prevent complications and improve patient outcomes.
PubCompare.ai's AI-driven platform helps researchers streamline their soft tissue infection studies by locating the best research protocols from literature, preprints, and patents.
Leverage our powerful comparrison tools to identify the optimal methods and prodcuts for your study, uncovering new insights to advance this critical area of medicine.

Most cited protocols related to «Soft Tissue Infection»

The Staphylococcus aureus strains used in this study were derived from a USA300 strain isolated from a skin and soft tissue infection in a detainee from the Los Angeles County Jail (LAC) (35 (link)). S. aureus strain LAC contains two plasmids, p01 (a 3.1-kb cryptic plasmid) and p03 (a 27-kb plasmid conferring resistance to erythromycin) (36 (link)). Both p01 and p03 were removed from LAC for two reasons. First, curing of these two plasmids eliminated potential plasmid incompatibility concerns with pFA545 and pBursa (see below), and second, curing ensured that the bursa aurealis transposon did not insert preferentially into plasmid DNA. For removal of p03, strain LAC was grown overnight in Tryptic soy broth (TSB) with shaking at 37°C without antibiotics. The next day, serial dilutions were made and the cells were plated on Trypic soy agar (TSA) plates without any antibiotics. Colonies arising after overnight incubation at 37°C were then replica plated onto plates with and without erythromycin (5 µg/ml). Those colonies unable to grow on erythromycin were chosen, and the loss of the plasmid was confirmed by performing plasmid analysis (Promega, Madison, WI). After confirmation that no major genomic rearrangements had occurred by pulsed-field gel electrophoresis (PFGE) (37 (link)), one colony, designated LAC-13C, was selected for subsequent manipulation.
We next cured p01 from LAC-13C utilizing a plasmid incompatibility strategy. First, a chimeric plasmid in which pCL84 (conferring tetracycline resistance) (38 (link)) and p01 were ligated together at their unique EcoRI sites was created. This plasmid, designated pJE06, was then electroporated into Escherichia coli DH5α, purified, and subsequently electroporated into S. aureus RN4220 (39 (link)). pJE06 was then transduced from RN4220 using bacteriophage ϕ11 (40 (link)) into LAC-13C using methods as described by McNamara (41 ). Plasmid preparations of the resulting tetracycline-resistant transductants were screened for the loss of p01 due to plasmid incompatibility (42 (link)). The resulting strains were then cured of pJE06 by following the process described above for removal of pUSA03 except that the colonies were screened for tetracycline sensitivity. Multiple tetracycline-susceptible derivatives were examined by PFGE to ensure that no chromosomal rearrangements were detected; one strain was selected for subsequent use and designated JE2.
Publication 2013
Agar Antibiotics Bacteriophages Cells CFC1 protein, human Chimera Chromosomes Deoxyribonuclease EcoRI derivatives Electrophoresis, Gel, Pulsed-Field Erythromycin Escherichia coli Gene Rearrangement Genome Hypersensitivity Jumping Genes Plasmids Promega Skin Soft Tissue Infection Staphylococcus aureus Staphylococcus aureus Infection Strains Synovial Bursa Technique, Dilution Tetracycline tryptic soy broth
The TIDOS project is an observational cohort study of short and long term infectious disease outcomes following a deployment-related traumatic injury. TIDOS cohort eligibility criteria include the following: active duty personnel or DoD beneficiary, ≥ 18 years of age, wounded or injured during deployment requiring return via Level IV at Landstuhl Regional Medical Center (LRMC), Landstuhl, Germany to a participating DoD hospital in the U.S., and providing informed consent or surrogate consent through a legally authorized representative. Participating Level V clinical sites include the National Naval Medical Center (NNMC), Bethesda, MD, Brooke Army Medical Center (BAMC), San Antonio, TX, and Walter Reed Army Medical Center (WRAMC), Washington DC. Consenting patients are enrolled in the study prior to discharge from one of the participating Level V hospitals. Participants are contacted periodically at 1, 3, 6, 12, 18, 24 months and then yearly for 5 years after discharge. Follow-up methods include subject interviews, web-based questionnaires, and query of DoD and Department of Veterans Affairs electronic healthcare databases.
Patient trauma history, injury severity scoring, and surgical management is obtained through selected data elements retrieved from the DoD Joint Theater Trauma Registry (JTTR).5 (link) The JTTR was established by the Assistant Secretary of Defense for Health Affairs as a means to provide an effective performance improvement tool to assess combat casualty care epidemiology, treatment, and outcome. An infectious diseases (ID) module to augment the JTTR was developed to capture infection-specific data throughout levels of care at participating hospitals. This ID-specific information includes diagnoses, treatments, and outcomes of bloodstream infections (BSI), clinical sepsis, bone and joint infections, skin and soft tissue infections (SSTI), central nervous system infections (CNS), intra-thoracic/pulmonary infections, and intra-abdominal infection. The JTTR/ID module captures data on all active duty military trauma patients admitted to Level IV and selected Level V facilities. A comprehensive assessment of the overall population and the representativeness of the TIDOS cohort were performed by analyzing de-identified JTTR/ID module data from non-enrolled trauma patients admitted through Level IV during the period. The study is approved by the institutional review boards of the Uniformed Services University of the Health Sciences and the St. Louis Veterans Administration Medical Center (VAMC).
This report includes subjects admitted to Level IV during the first three months of the study (June 1 – August 31, 2009) along with subject experience through Level V hospitalization (for those admitted to participating Level V sites) and up to 6-months of follow-up post Level V discharge (for those consenting to follow-up).
Publication 2011
Bones Central Nervous System Infection Communicable Diseases Diagnosis Eligibility Determination Ethics Committees, Research Health Services, University Hospitalization Infection Injuries Intraabdominal Infections Joints Lung Military Personnel Operative Surgical Procedures Patient Discharge Patients Persistent Infection Sepsis Septicemia Skin Soft Tissue Infection Veterans Wounds and Injuries
First we searched for relevant guidelines, using Medline, National Guideline Clearinghouse, Cochrane Health Technology Assessment, National Institutes of Health Consensus Development, and the US Preventative Services Task Force. On the basis of a review of those guidelines, each team developed a series of key questions. Examples of these key questions are “What is the utility of examination of urine for pyuria for the diagnosis of symptomatic urinary tract infection?” and “What is the diagnostic accuracy of pulse oximetry for nursing home pneumonia?” These key questions further guided the evidence review used to revise the existing surveillance criteria. Next, a search of the primary literature was performed, using Medline, CINAHL, Embase, Cochrane Systematic Reviews, and the Cochrane Controlled Clinical Trials Registry. Examples of key search terms include the following: nursing home, long-term care, aged, skilled nursing facility, older adults, elderly, fever, healthcare-associated infection, pneumonia, influenza, respiratory tract infection, functional impairment, confusion, leukocyte count, pulse oximetry, urinary tract infection, bacteriuria, urine culture, gastroenteritis, diarrhea, Clostridium difficile, norovirus, cellulitis, soft tissue infection, pressure ulcer, scabies. A line listing of articles that met the search criteria and were included in the final analyses is available upon request from the authors.
Publication 2012
Aged Cellulitis Clostridium difficile Diagnosis Diarrhea Fever Gastroenteritis Infections, Hospital Influenza Leukocyte Count Long-Term Care Norovirus Oximetry, Pulse Pneumonia Pressure Ulcer Respiratory Tract Infections Scabies Soft Tissue Infection Technology Assessment, Biomedical Urinalysis Urinary Tract Infection Urine
The purpose of this study is to collect real-world data about the non-controlled prescribing use and impact of daptomycin. Therefore, a non-interventional, multicentre, retrospective design was chosen. Investigators were asked to voluntarily include as many patients as available. Patients could be retrospectively included if they were treated with at least one dose of daptomycin and if all relevant information was entered in the case report form (CRF). Patients who received daptomycin as part of a controlled clinical trial were not eligible for inclusion. Investigators at >100 institutions across Europe collected anonymous demographic, antibiotic, microbiological and clinical data from medical records using a standardized CRF and protocol. Argentinean sites were also allowed to participate, as overall enrolment predictions were uncertain and daptomycin had just been approved in that country. Written informed consent that complies with the ICH Good Clinical Practice guideline was obtained if required by the institutional review board or ethics committee and/or local data privacy regulations, and the protocol was approved by the health authority and the institutional review board or ethics committee, as required, in each country. Investigators were trained on the CRF and received written instructions to further guide the collection of patient data. Each patient is uniquely identified in the study by a combination of his/her centre number and patient number. All data are entered on standardized CRFs exclusively according to the investigator's judgement. Data from the CRFs are entered into the study database by certified contract research organization staff working on behalf of the sponsor. After database lock, all available data were distributed to all authors of the manuscript, who take part in scheduled publication committee meetings. All information collected reflected standard practice in each site. There was no intervention or restriction in clinical practice. Patient data can be recorded into this registry after a minimum of 30 days from the end of daptomycin therapy to permit the capture of AEs/serious AEs (SAEs). The CRFs collected the following information: treatment period; demographics; underlying diseases; pregnancy; neutropenia; antimicrobials; use of other antibiotics and statins; renal function; creatine phosphokinase (CPK) concentrations; diagnosis and current infection details; doses of current antibiotic treatment; duration of inpatient and/or outpatient treatment; outcomes; AEs and SAEs occurring between treatment onset and 30 days after last dose of daptomycin; and discharge information. No instructions were provided concerning drug discontinuation, study completion or post-study treatment. However, the reasons for study drug discontinuation, reason for completion of daptomycin treatment and antibiotic use after daptomycin treatment were captured. In cases of multiple infection, investigators entered the type of infection in order of clinical significance (i.e. primary or secondary infection) for which the patient received daptomycin.
In the database, the primary infection was always automatically assigned to the disease of higher hierarchy according to the following predefined severity classification (in order of most to least severe): endocarditis; osteomyelitis; bacteraemia; other [CNS infection, foreign body/prosthetic infection, metastatic abscess, necrotizing fasciitis, necrotizing infection, surgical/non-surgical antibiotic prophylaxis, septic arthritis and urinary tract infection (UTI)/pyelonephritis]; cSSTI; uncomplicated skin and soft tissue infection (uSSTI). This classification was needed to allow for analysis standardization and was based on experts’ and sponsor's judgement taking into consideration clinical prognosis, probability of microbiology eradication and consequences of treatment failure. Safety analysis included all reports of AEs, the severity of which was determined by the investigators. AEs were recorded regardless of their relationship to daptomycin therapy. The overall methodology of this registry, including the definitions mentioned in the following section, is aligned with the CORE registry methodology. It is possible to merge the databases to perform combined or comparative analyses between different regions, as recently published by Gonzalez-Ruiz et al.9
Publication 2011
Abscess Antibiotic Prophylaxis Antibiotics Arthritis, Infectious Bacteremia Care, Ambulatory Central Nervous System Infection Clinical Reasoning Creatine Kinase Daptomycin Diagnosis Endocarditis Ethics Committees Ethics Committees, Research Foreign Bodies Hydroxymethylglutaryl-CoA Reductase Inhibitors Infection Inpatient Kidney Leukopenia Microbicides Necrotizing Fasciitis Operative Surgical Procedures Osteomyelitis Patient Discharge Patients Pharmaceutical Preparations Pregnancy Prognosis Pyelonephritis Safety Secondary Infections Skin Soft Tissue Infection Therapeutics Urinary Tract Infection
Blood cultures were collected and processed free of charge in healthcare facilities in the capital, Kinshasa, and the Bas-Congo province (referral hospital of Kisantu) and later extended to sentinel hospitals in the Oriental Province (University Hospital Kisangani with affiliated hospitals and health centers) and the Equateur Province (referral hospital of Bwamanda). Over the years, this network was extended with other partners, and blood cultures were also added to the diagnostic tools of the outbreak investigation teams of INRB in collaboration with the Ministry of Health. Indications of blood culture sampling were, outside the neonatal period, a body temperature of ≥38.0°C or ≤35.5°C (axillary) or history of fever during the last 48 hours and/or (1) signs of severity such as hypotension, confusion, or increased respiratory rate; (2) suspicion of severe localized infections (pneumonia, meningitis, complicated urinary tract infection, osteomyelitis and arthritis, severe skin and soft tissue infections, gynecological and abdominal infections/peritonitis); or (3) suspicion of other severe infection: sepsis, typhoid fever, and severe malaria. Basic demographic and clinical data were recorded. In adults, blood cultures consisted of 2 × 10 mL of blood sampled in aerobic BACT/ALERT bottles (bioMérieux, Marcy-L’Etoile, France); for children (defined as ≤14 years old), 1–4 mL was sampled in pediatric BACT/ALERT bottles. Grown blood cultures were worked up for identification according to conventional biochemical methods and serotyping (for antibiotic susceptibility testing, see below). For reasons of integration in clinical care and capacity building, blood cultures were preferentially worked up in the participating referral hospitals. However, when no competent laboratory services were available, samples were transported to INRB for workup. Isolates were stored for in-depth reference testing, and the results of the blood culture isolates and their resistance patterns were registered. Coagulase-negative Staphylococcus, Corynebacterium species, Micrococcus species, Cutibacterium species, and Lactobacillus species were considered as environmental or skin contaminants; the other bacteria were classified as pathogens. The term “suspected bloodstream infection (BSI) episode” was used to indicate all blood cultures in a single patient sampled within a 2-week period according to the indications mentioned above. A BSI episode was considered as culture confirmed when a pathogen was isolated in at least 1 of the bottles. Supplementary Document 1 provides a detailed overview of the workflow, criteria, and definitions used in the sampling, and the processing and reporting of the blood cultures.
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Publication 2019
Adult Antibiotics Arthritis Asian Persons Axilla Bacteria Bacteria, Aerobic Blood Blood Culture Body Temperature Child Coagulase Corynebacterium Diagnosis Fever Focal Infection Hospital Referral Infant, Newborn Infection Intraabdominal Infections Lactobacillus Malaria Meningitis Micrococcus Osteomyelitis Pathogenicity Patients Peritonitis Pneumonia Sepsis Septicemia Skin Soft Tissue Infection Staphylococcus Susceptibility, Disease Typhoid Fever Urinary Tract Infection

Most recents protocols related to «Soft Tissue Infection»

The secondary outcomes were the overall incidence of DVT, rate of blood transfusion, the incidence of wound complications and superficial soft tissue infections, leg circumference and the visual analog scale (VAS) score. The circumference of the leg was measured before surgery and at postoperative day 7 by physiotherapists at the maximum circumference of the lower leg. The VAS score at rest was measured periodically from the following day to five days after surgery at 6:00 AM, 12:00 PM, and 8:00 PM. The VAS score during activity was defined as the strongest pain experienced during physical therapy.
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Publication 2023
Blood Transfusion Leg Operative Surgical Procedures Pain Physical Therapist Soft Tissue Infection Therapy, Physical Visual Analog Pain Scale Wounds
Ten consecutive, non-duplicate SCCmec type IV MRSA strains isolated from skin and soft tissue infections (SSTIs) swabs between December 2020 and April 2021 were obtained from the Microbiology Laboratory at Salmaniya Medical Complex (SMC), Kingdom of Bahrain. All strains were identified as MRSA via the BD Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD, USA); SCCmec typing was conducted via multiplex PCR in accordance with Boye et al. [8 (link)]. Primers, reaction conditions and gel pictures can be seen in File S2, available in the online version of this article).
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Publication 2023
Adjustment Disorders Diagnosis Methicillin-Resistant Staphylococcus aureus Multiplex Polymerase Chain Reaction Oligonucleotide Primers Skin Soft Tissue Infection Strains Vision
Invasive disease was defined by the isolation of GAS from a normally sterile site (blood, bone, pleural fluid, synovial fluid, peritoneal fluid, or cerebrospinal fluid (CSF)).
Probable invasive disease was defined as an unwell patient with GAS isolated from a non-sterile site (deep skin or retropharyngeal aspiration) who required one or more of the following: hospitalization for intravenous antibiotics, surgery, or admission to the intensive care unit (ICU).
Clinical syndromes of piGAS and iGAS disease were categorized as isolated bacteremia, pneumonia/empyema, skin and soft tissue infection (SSTI) with or without bacteremia, NF, STSS, septic arthritis, osteitis, bursitis, abdominal/peritoneal infection, meningitis, pharyngeal abscess with and without bacteremia, and endometritis (pregnancy or not pregnancy related).
Clinical syndromes are presented in Supplementary Information 1. In case of concurrent diagnosis in the same patient, the most severe diagnosis was retained.
IGAS infections were considered healthcare related if they occurred at least 48 h after the time of admission or if the patient underwent surgery within the 7 days preceding the onset of iGAS. IGAS or piGAS infections were considered associated to chickenpox if it occurred within a maximum time span of 7 days from the onset of the varicella infection. The pediatric population was limited to children between 0 and 16 years. An adult was defined as a patient at least 17 years old.
The 2010 case definition [15 ] based on two major criteria (hypotension and the involvement of at least two organs/systems) was applied for the diagnosis of STSS.
Publication 2023
Abscess Adult Antibiotics Arthritis, Infectious Bacteremia Blood Bones Bursitis Cerebrospinal Fluid Chickenpox Child Diagnosis Empyema, Pleural Endometritis Hospitalization Infection Intraabdominal Infections Meningitis Operative Surgical Procedures Osteitis Patients Peritoneal Fluid Peritoneum Pharynx Pleura Pregnancy Skin Soft Tissue Infection Sterility, Reproductive Syndrome Synovial Fluid Tetradecyl Sulfate, Sodium
This was a retrospective study carried from September 2018 to March 2020 in Shanghai Sixth People’s Hospital, and 43 patients (25 males and 18 females; average age, 38.4 years; age range, 20–65 years), who had persistent ankle pain and associated activity restrictions despite at least 6 months of nonoperative management, mechanical instability with manual anterior drawer test (ADT) stress maneuvers, with or without recognizable tenderness on palpation medial to the tibialis anterior tendon, and follow-up for at least 2 years, were assessed for study inclusion. Preoperative plain radiography and magnetic resonance imaging (MRI) were also collected. All patients agreed to arthroscopic ATFL repair and were separated into two groups: AMAI group (AMAI including intraoperative AMAO resection; 15 males and 14 females) and pure CLAI (with AMAO but without AMAI, no AMAO resection; 10 males and 4 females). This grouping method was based on whether AMAO was resected arthroscopically and the related data were gathered by two senior surgeons at the last follow-up. For each indicator, each surgeon averaged three measurements to determine the measurement result, and the final result was the average of the two surgeons’ measurement results. Patients were excluded if they had ALAI, manifest tibiotalar osteoarthritis, severe peripheral angioneuropathy, soft tissue infection at the surgical site, or a history of mental illness. All participants provided written informed consent, and the study was approved by our institution’s Ethics Committee.
Preoperative and 2-year follow-up measurements of ankle dorsiflexion, the American Orthopedic Foot and Ankle Society (AOFAS) ankle–hindfoot score, and the visual analog scale (VAS) were taken. Ankle dorsiflexion measurement was taken with a goniometer (Xu Lang Xing, China). Participants were sitting on examination table during testing, with the ankle and foot suspended over the end of the examining table and the subtalar joint in neutral position. Participants were instructed to actively dorsiflex the ankle to an extreme. The axis of the goniometer was placed near to the lateral malleolus, the stationary arm was aligned with the head of fibula, and the movable arm was aligned parallel to the plantar aspect of the calcaneus and fifth metatarsal [24 (link)]. In all cases, the same pre- and postoperative questionnaires were used and the ankle dorsiflexion angles were measured.
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Publication 2023
Ankle Arthroscopes Calcaneus Degenerative Arthritides Dorsiflex Epistropheus Examination Tables Exercise Tests Females Fibula Foot Head Institutional Ethics Committees Males Mental Disorders Metatarsal Bones Operative Surgical Procedures Pain Palpation Patients Soft Tissue Infection Subtalar Joint Surgeons Tendons Tibial Muscle, Anterior Visual Analog Pain Scale X-Rays, Diagnostic
We retrospectively analyzed the surgical episodes of elective foot and ankle surgeries in our foot center; an orthopedic referral center at the Balgrist University Hospital in Zurich, Switzerland, from January 2014 to September 2022. We used data mining from the hospital’s own medical databases and verified the SSI by opening individual electronic files. We included all patients older than 18 years of age with elective foot and ankle surgeries and excluded emergency surgeries understood as any injury that requires immediate medical care [19 (link)], such as open or displaced fractures or surgeries performed for community-acquired or nosocomial infections (gangrene, severe soft tissue infection), and severe ischemia. We divided the cohort into two groups of diabetic and non-diabetic patients. We studied the key variables related to possible risk factors for SSI, including duration of the surgery, type of elective surgery, more than two previous surgeries, surgery with foreign material (osteosynthesis, external material), the ASA score, and the presence of a chronic wound in diabetics. The local ethics committee approved our retrospective study (BASEC 2022-01755). Patients who did not provide their general informed consent upon hospitalisation for surgery were not analyzed.
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Publication 2023
Ankle Elective Surgical Procedures Emergencies Foot Foreign Bodies Fracture, Bone Fracture Fixation, Internal Gangrene Hospital Referral Infections, Hospital Injuries Ischemia Operative Surgical Procedures Patients Regional Ethics Committees Soft Tissue Infection Wounds

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More about "Soft Tissue Infection"

Soft tissue infections are a diverse group of conditions affecting the skin, muscles, and connective tissues.
These infections can range from minor abrasions to severe, life-threatening necrotizing fasciitis.
Prompt diagnosis and appropriate treatment are crucial to prevent complications and improve patient outcomes.
Researchers can leverage PubCompare.ai's AI-driven platform to streamline their soft tissue infection studies.
This platform helps locate the best research protocols from literature, preprints, and patents.
Researchers can utilize powerful comparison tools to identify the optimal methods and products for their studies, uncovering new insights to advance this critical area of medicine.
Some key subtopics and related terms in the field of soft tissue infections include: - Skin and soft tissue infections (SSTIs) - Cellulitis - Erysipelas - Abscess - Necrotizing fasciitis - Myositis - Pyomyositis - Virulence factors - Antimicrobial resistance - Diagnostic tools like Vitek 2, MALDI-TOF MS, and MALDI-TOF mass spectrometry - Reference strains like S. epidermidis ATCC 35984, S. aureus ATCC 29213, and S. aureus ATCC 25923 - Genotyping methods like Identibac S. aureus Genotyping - Tissue culture techniques using Cytodex 3 microcarrier beads - Antimicrobial agents like Bacitracin and Polymyxin B By utilizing PubCompare.ai's AI-powered platform, researchers can streamline their soft tissue infection studies, identify the best research protocols, and uncover new insights to advance this critical area of medicine.