Log In Sign up
The largest database of trusted experimental protocols
> Disorders > Disease or Syndrome > Yellow Fever

Yellow Fever

Yellow fever is a serious and potentially deadly viral disease transmitted by infected mosquitoes.
It is characterized by fever, chills, loss of appetite, nausea, muscle pain, and headache.
In severe cases, it can lead to liver damage, bleeding, and organ failure.
PubCompare.ai can help optimize your Yellow Fever research by locating the best protocols from literature, pre-prints, and patents.
Their AI-driven comparisons enhance reproducibility and accuracy, ensuring you find the most effective strategies to advance your work in this important area of study.
Explore PubCompare.ai and take your Yellow Fever research to new heights.

Most cited protocols related to «Yellow Fever»

1
Modeling Cause-Specific Mortality with Insufficient Data

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2017
African Trypanosomiasis Hepatitis A Leishmaniasis, Visceral Malaria Measles Paratyphoid Fever Pertussis Syphilis Typhoid Fever Yellow Fever Zika Virus Infection
2
Comprehensive Assessment of Disease Burden

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2012
Acquired Immunodeficiency Syndrome Acute Disease African Trypanosomiasis Appendicitis Behavior Disorders Care, Prenatal Cerebrovascular Accident Child Cholera Chronic Kidney Diseases Congenital Abnormality Dementia Dengue Fever Disease, Chronic Drug Abuser Epilepsy Households Injuries Intellectual Disability Leprosy Liver Cirrhosis Malignant Neoplasms Measles Mental Health Multiple Sclerosis Myocardial Infarction Outpatients Pancreatitis Patient Discharge Patients Pertussis Pneumoconiosis Population Group Projective Techniques Respiratory Diaphragm Schistosomiasis sequels Skin Diseases Syphilis Tuberculosis Vision Woman Yellow Fever
3
Mosquito Sampling in Benin, West Africa
Sampling of mosquitoes was done in 28 villages by human landing catches using tubes that were plugged with cotton. Mosquitoes collection was carried out during 5 surveys from October 2007 to May 2008 (2 in the beginning of rainy periods and 3 in dry periods) every 6 weeks both indoors and outdoors at 4 sites per village from 10 p.m. to 6 a.m. and for two consecutive nights per survey (i.e. 16 person-nights per village per survey). Teams of collectors were rotated among the collection points on different collection nights to minimize sampling bias. Ethical clearance was given for the study by the National Ethical Committee in Benin (Comité National Provisoire d'Ethique pour la Recherche en Santé) and IRD ethical committee (Comité Consultatif de Déontologie et d'Ethique). Collectors gave prior informed consent and they were vaccinated against yellow fever. Since study was done in area where malaria is endemic, adult collectors that already acquired immunity against malaria parasites, did not received chemoprophylaxis, but were medically supervised by local physicians in case of illness.
Mosquitoes were identified on the field to species level using morphological criteria according to the identification keys [10 -12 ]. All mosquitoes belonging to the An. gambiae complex or An. funestus group were stored in individual tubes with silica gel and preserved at -20°C in the laboratory.
Djènontin A., Bio-Bangana S., Moiroux N., Henry M.C., Bousari O., Chabi J., Ossè R., Koudénoukpo S., Corbel V., Akogbéto M, & Chandre F. (2010). Culicidae diversity, malaria transmission and insecticide resistance alleles in malaria vectors in Ouidah-Kpomasse-Tori district from Benin (West Africa): A pre-intervention study. Parasites & Vectors, 3, 83.
Full text: Click here
Publication 2010
Adaptive Immunity Adult butocin Chemoprevention Culicidae Gossypium Homo sapiens Malaria Parasites Physicians Rain Silica Gel Yellow Fever
4
Dengue and Flavivirus Antibody Profiling
Dengue antibody-positive human serum samples from children and adults were obtained from patients with dengue and from persons who had participated in clinical trials of the Sanofi Pasteur recombinant CYD tetravalent dengue vaccine candidate. Yellow fever (YF)– and Japanese encephalitis (JE)–antibody positive human serum samples were obtained from healthy adult donors who received vaccines against YF virus (YF-VAX®) or JE virus (JE-VAX™), respectively. Dengue antibody-negative human serum samples were obtained from healthy adult donors from non-endemic dengue areas.19 (link) Sample identifiers were removed and new sample identification numbers were issued. Samples were heat inactivated for 30 minutes at 56°C before use. Quality control samples were used in each assay run and demonstrated to be suitable for their intended purpose by consistently performing within the previously established neutralization titer limits, and served to monitor assay performance.
Timiryasova T.M., Bonaparte M.I., Luo P., Zedar R., Hu B.T, & Hildreth S.W. (2013). Optimization and Validation of a Plaque Reduction Neutralization Test for the Detection of Neutralizing Antibodies to Four Serotypes of Dengue Virus Used in Support of Dengue Vaccine Development. The American Journal of Tropical Medicine and Hygiene, 88(5), 962-970.
Publication 2013
Adult Biological Assay Child Dengue Fever Donors Fever Vaccine, Yellow Homo sapiens Immunoglobulins Japanese Encephalitis Patients Serum Vaccine, Dengue Vaccines Virus Virus, Japanese Encephalitis Yellow Fever Yellow fever virus
5
Functional Modulation of Human Monocytes
To enable analysis of the different functional programs of monocytes, human primary monocytes were purified from three to six healthy human donors (depending on experiment) by first isolating peripheral blood mononuclear cells by differential centrifugation over Ficoll-Paque, followed by depletion of T-cells, B-cells and NK-cells with anti-CD3, CD19 and CD56-coated beads, respectively (Fig. S1). Macrophages (Mf) were obtained by in vitro culture of the purified monocytes (Mo) for 6 days in cell culture medium enriched with human serum (10%). Monocyte tolerance, a state akin to immune paralysis during bacterial sepsis or endotoxin shock, was induced in vitro by exposing the purified monocytes to lipopolysaccharide (LPS) for 24 h (9 (link)). Monocyte training, a functional state in which cells respond more strongly to microbial stimuli that mirrors the non-specific protective effects of live microorganism vaccination (e.g. BCG, measles, yellow fever, (15 (link))) (Fig. 1A, B) was induced in vitro by exposing the purified monocytes to β-glucan for 24 h (Fig. 1B) (10 (link)). The LPS or β-glucan was then washed out from the system and the cells were incubated in enriched cell cultured medium for five days.
Saeed S., Quintin J., Kerstens H.H., Rao N.A., Aghajanirefah A., Matarese F., Cheng S.C., Ratter J., Berentsen K., van der Ent M.A., Sharifi N., Janssen-Megens E.M., Huurne M.T., Mandoli A., van Schaik T., Ng A., Burden F., Downes K., Frontini M., Kumar V., Giamarellos-Bourboulis E.J., Ouwehand W.H., van der Meer J.W., Joosten L.A., Wijmenga C., Martens J.H., Xavier R.J., Logie C., Netea M.G, & Stunnenberg H.G. (2014). Epigenetic programming during monocyte to macrophage differentiation and trained innate immunity. Science (New York, N.Y.), 345(6204), 1251086.
Publication 2014
B-Lymphocytes Bacteria beta-Glucans Cell Culture Techniques Cells Centrifugation Donors Ficoll Homo sapiens Immune Tolerance Lipopolysaccharides Macrophage Measles Monocytes Muromonab-CD3 Natural Killer Cells PBMC Peripheral Blood Mononuclear Cells Sepsis Serum Shock, Endotoxic T-Lymphocyte Vaccines, Attenuated Yellow Fever

Most recents protocols related to «Yellow Fever»

1
Epidemics Surveillance and Management in Guinea
The study was conducted in Guinea, a West African country with a population of approximately 13 million, 84% of whom live in rural areas.48 The Guinean health system has three levels—local (38 health districts)—intermediate (eight health regions)—and central (MoH).49 The structural organisation of the central level includes the National Agency for Health Security (ANSS), as an attached service. The ANSS, created on 4 July 2016 by presidential decree (N°:D/2016, 205/PRG/SGG)50 after the EVD outbreak, is in charge of the prevention, surveillance and management of epidemic diseases in Guinea. It implements the strategic orientations of the MoH in terms of health security in the country.
Between 2014 and 2021, Guinea experienced several outbreaks such as EVD, Meningitis, Measles, Yellow Fever, COVID-19, cVDPV2, Lassa Fever and Marburg virus disease. Some of these outbreaks spread over all the health districts, and others affected only one or some health districts, as mapped in figure 1 using the quantum geographic information system version 3.6. The two haemorrhagic fevers (EVD and Marburg virus disease) originated from the N’Zérékoré health region, where there exist two forests (Ziama and Diécké) considered among the world’s last remaining primary forests.51 (link)
Millimouno T.M., Meessen B., Put W.V., Garcia M., Camara B.S., Christou A., Delvaux T., Sidibé S., Beavogui A.H, & Delamou A. (2023). How has Guinea learnt from the response to outbreaks? A learning health system analysis. BMJ Global Health, 8(2), e010996.
Publication 2023
COVID 19 Disease Management Disease Outbreaks Epidemics Forests Hemorrhagic Fevers, Viral Lassa Fever Marburg Virus Disease Measles Meningitis Mental Orientation Morphogenesis Nervous System, Autonomic Secure resin cement West African People Yellow Fever
2
Serological Detection of Zika Virus
Serology for ZIKV was performed with the “Anti-Zika Virus ELISA (IgG)” kit (EI 2668–9601 G, EUROIMMUN Schweiz AG, Luzern, Switzerland), according to the manufacturer’s specifications. Confirmation of positive samples was performed using a custom designed flavivirus mosaic Indirect Immunofluorescence Test (IIFT) (EUROIMMUN Schweiz AG, Luzern, Switzerland), in which cells infected with Zika, Dengue (I-IV), West Nile, Yellow fever and Japanese encephalitis viruses were used to detect their antigens.
Chui Y.C., Baud D., Fahmi A., Zumkehr B., Vouga M., Pomar L., Musso D., Thuong B.C., Alves M.P, & Stojanovic M. (2023). Absence of Zika virus among pregnant women in Vietnam in 2008. Tropical Diseases, Travel Medicine and Vaccines, 9, 4.
Full text: Click here
Publication 2023
Antigens Cells Dengue Fever Encephalitis Viruses, Japanese Enzyme-Linked Immunosorbent Assay Flavivirus Indirect Immunofluorescence Yellow Fever Zika Virus
3
Recombinant Protein Expression and Purification
The Yellow fever virus envelope protein antigen, YFE‐1, and the B. anthracis protective antigen, PA83, were targeted to the endoplasmic reticulum and carried poly‐histidine tags. For expression analyses, all leaves infiltrated with Agrobacteria were collected and ground for protein extraction. Soluble proteins were extracted in a Tris‐based extraction buffer for YFE‐1 or a phosphate‐based extraction buffer for PA83. Target expression levels were then assessed by SDS–PAGE followed by immunoblot analysis using a tetrahistidine‐specific monoclonal antibody (Qiagen), with a dilution series of a recombinant protein with a poly‐histidine tag providing a standard curve for quantification. For target protein purification, extracts were clarified by filtration through miracloth and centrifugation at 6800 g, and the target protein was recovered by immobilized metal affinity chromatography. YFE‐1 and PA83 yields were then assessed by SDS–PAGE followed by staining with Coomassie blue, with a dilution series of BSA providing a standard curve for quantification of an appropriate dilution of each target molecule that lay within the standard curve. The G:BOX Mini Gel and Blot Documentation System (Syngene) was used for target quantification by immunoblot analysis and SDS–PAGE.
Wang X., Prokhnevsky A.I., Skarjinskaia M., Razzak M.A., Streatfield S.J, & Lee J. (2023). Facilitating viral vector movement enhances heterologous protein production in an established plant system. Plant Biotechnology Journal, 21(3), 635-645.
Publication 2023
Agrobacterium Antigens Bacillus anthracis Buffers Centrifugation Chromatography, Affinity Coomassie blue Endoplasmic Reticulum Filtration Gene Products, env Immunoblotting Metals Monoclonal Antibodies Phosphates polyhistidine Proteins Recombinant Proteins SDS-PAGE Staphylococcal Protein A Technique, Dilution Tromethamine Viral Envelope Proteins Yellow Fever Yellow fever virus
4
Generation of Fluorescent PDLP5 Fusion Constructs
Fluorescently tagged fusion constructs of PDLP5 (UniProt Q8GUJ2) or its cytoplasmic tail mutants were produced using overlapping PCR by Phusion high‐fidelity DNA polymerase (New England Biolabs), and purified DNA fragments were subsequently cloned into the Gateway entry vector pENTR/D‐TOPO (ThermoFisher Scientific) and destination vector pGWB. To create pBI‐D vector expression cassettes, sequences from the pBI121 binary vector were replaced by expression cassettes containing a Cauliflower mosaic virus (CaMV) 35 S promoter with dual enhancers, a 5′ nontranslated leader sequence from Tobacco etch virus (Carrington and Freed, 1990 (link)), and a CaMV 35 S terminator. The PDLP5 3C‐3A mutant was cloned into the binary vector pBI‐D, introduced into the Agrobacterium tumefaciens strain GV3101 by electroporation, and used to create transgenic N. benthamiana lines. Optimized Yellow fever virus envelope protein (YFE‐1) (UniProt P03314) and B. anthracis protective antigen (PA83) (UniProt P13423) genes were cloned into a pGreen‐based expression vector carrying TMV genome sequences from pBID4 (Musiychuk et al., 2007 ). The resulting constructs were introduced into the A. tumefaciens strain AGL1 by electroporation. All constructs were confirmed by Sanger sequencing before agro transformation. Additional information regarding plasmids and vectors used in the current study is provided in Table S1.
Wang X., Prokhnevsky A.I., Skarjinskaia M., Razzak M.A., Streatfield S.J, & Lee J. (2023). Facilitating viral vector movement enhances heterologous protein production in an established plant system. Plant Biotechnology Journal, 21(3), 635-645.
Publication 2023
Agrobacterium tumefaciens Animals, Transgenic Antigens Bacillus anthracis Cauliflower Mosaic Virus Cloning Vectors Cytoplasm DNA-Directed DNA Polymerase Electroporation Gene Products, env Genes Genome Plasmids Strains Tail Tobacco etch virus Topotecan Viral Envelope Proteins Yellow Fever Yellow fever virus
5
Vaccination Characteristics and Indications

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Adolescent Chickenpox Child Cholera Diagnosis Ethnicity Females Inpatient Japanese Encephalitis measles, mumps, rubella, varicella vaccine Measles-Mumps-Rubella Vaccine Measles Vaccine Obstetric Delivery Poliovirus Vaccines Rubella Vaccination Vaccines Vaccines, Typhoid Woman Yellow Fever Youth

Top products related to «Yellow Fever»

1
Qiaamp viral rna mini kit by Qiagen
Sourced in Germany, United States, United Kingdom, France, Spain, Japan, China, Netherlands, Italy, Australia, Canada, Switzerland, Belgium
The QIAamp Viral RNA Mini Kit is a laboratory equipment designed for the extraction and purification of viral RNA from various sample types. It utilizes a silica-based membrane technology to efficiently capture and isolate viral RNA, which can then be used for downstream applications such as RT-PCR analysis.
2
Qiamp viral rna mini kit by Qiagen
Sourced in Germany, United States, United Kingdom, France, Canada
The QIAamp Viral RNA Mini Kit is a column-based nucleic acid extraction kit designed for the isolation of viral RNA from various sample types, including plasma, serum, and cell-free body fluids. The kit utilizes a silica-membrane technology to efficiently capture and purify viral RNA, making it suitable for downstream applications such as RT-PCR and RT-qPCR.
3
Realstar dengue rt pcr kit 2 by Altona Diagnostics
Sourced in Germany
The RealStar® Dengue RT-PCR Kit 2.0 is a real-time reverse transcription-polymerase chain reaction (RT-PCR) test for the detection of the Dengue virus. It is designed to amplify and detect specific genomic regions of the virus.
4
Realstar yellow fever virus rt pcr kit 1 by Altona Diagnostics
Sourced in Germany
The RealStar® Yellow Fever Virus RT-PCR Kit 1.0 is a real-time RT-PCR test for the qualitative detection of yellow fever virus RNA.
5
Stamaril by Sanofi
Sourced in France
Stamaril is a sterile, freeze-dried vaccine used for the prevention of yellow fever. It contains the live, attenuated yellow fever virus.
6
Biorobot mdx by Qiagen
Sourced in Germany
The BioRobot MDx is an automated workstation designed for high-throughput nucleic acid purification. It performs sample preparation, extraction, and purification for downstream molecular diagnostic applications. The BioRobot MDx is capable of processing multiple samples simultaneously, ensuring efficient and consistent results.
7
Goat anti mouse igg hrp by Merck Group
Sourced in United States, Germany
Goat anti-mouse IgG-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed for the detection of mouse primary antibodies in various immunoassay applications.
8
Immuneaccess by Adaptive Biotechnologies
ImmuneACCESS is a lab equipment product that enables the analysis of immune receptor sequences. It is designed to provide researchers with a comprehensive view of the adaptive immune system.
9
Goat anti mouse igg ap by Merck Group
Sourced in United States
Goat anti-mouse IgG-AP is a laboratory reagent used in immunoassays and other immunochemical techniques. It is an antibody preparation that specifically recognizes and binds to mouse immunoglobulin G (IgG) antibodies, and is conjugated with the enzyme alkaline phosphatase (AP).
10
Disodium hydrogen phosphate by Merck Group
Sourced in Germany, United States, India, France, Italy, Spain, China, Australia, Canada, Belgium, United Kingdom, Thailand
Disodium hydrogen phosphate is a chemical compound used as a buffer and desiccant in various laboratory applications. It is a white crystalline solid that is soluble in water. The primary function of disodium hydrogen phosphate is to maintain a specific pH level in solutions and to absorb moisture in order to preserve the stability and integrity of laboratory samples and reagents.

More about "Yellow Fever"