BSC1 (monkey kidney epithelial) cells stably expressing rat brain clathrin light chain-EGFP (kindly provided by T. Kirchhausen, Harvard Medical School, Boston, MA) were cultured and prepared as specified in Supplementary Note 13 online. For live cell imaging, BSC1 cells were plated on glass coverslips, and through-the-objective TIR-FM was performed on a Nikon TE2000U inverted microscope using a 100X/1.45NA oil-immersion objective. Images were captured at 0.5 Hz with 200ms exposure time using a Hamamatsu Orca II-ERG.
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Submersion
Submersion
Submersion is the process of being completely immersed in a liquid, typically water.
This can occur in various situations, such as swimming, diving, or accidental drowning.
Submersion can lead to respiratory distress, hypothermia, and other life-threatening conditions if not addressed promptly.
It is an important concept in fields such as emergency medicine, water safety, and aquatic sports.
Proper precautions and training are essential to prevent and manage submersion-related incidents and their potentail consequences.
This can occur in various situations, such as swimming, diving, or accidental drowning.
Submersion can lead to respiratory distress, hypothermia, and other life-threatening conditions if not addressed promptly.
It is an important concept in fields such as emergency medicine, water safety, and aquatic sports.
Proper precautions and training are essential to prevent and manage submersion-related incidents and their potentail consequences.
Most cited protocols related to «Submersion»
Brain
Cells
Clathrin Light Chains
Epithelial Cells
Kidney
Microscopy
Monkeys
Orcinus orca
Submersion
Images were kindly provided by Javier Frias Aldeguer and Nicolas Rivron of Hubrecht Institute for Developmental Biology and Stem Cell Research and Li Linfeng of MERLN Institute for Technology-Inspired Regenerative Medicine. As per Rivron and colleagues [33 (link)], mouse embryos (3.5 dpc) were fixed right after isolation from the mother’s uterus. Fixation was performed using 4% PFA in RNAse-free PBS containing 1% acetic acid. ViewRNA ISH Cell Assay kit (cat# QVC0001) was used for performing smFISH on the embryos. The protocol includes steps of permeabilization and protease treatment as well as probes, preamplifier, amplifier, and label hybridizations. Embryos were then mounted in Slowfade reagent (Thermofisher cat# S36937) and directly imaged in a PerkinElmer Ultraview VoX spinning disk microscope in confocal mode by using a 63×/1.40 NA oil immersion lens.
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2-5A-dependent ribonuclease
Acetic Acids
Biological Assay
Cells
Crossbreeding
Embryo
isolation
Lens, Crystalline
Microscopy
Mothers
Mus
Peptide Hydrolases
Submersion
Uterus
Brain
Cells
Clathrin Light Chains
Epithelial Cells
Kidney
Microscopy
Monkeys
Orcinus orca
Submersion
Mice were placed on a warm blanket (37°C) and kept anesthetized with 0.5% isoflurane and sedated with chlorprothixene (20-40 μL at 0.33 mg/ml, i.m.)30 (link). Imaging was performed using a custom-built two-photon microscope (designs available at research.janelia.org/Svoboda) equipped with a resonant galvo scanning module (Thorlabs), controlled by ScanImage (scanimage.org)60 (link). The light source was a Mai Tai femtosecond pulsed laser (Spectra-Physics) running at 940 nm. The objective was a 16× water immersion lens (Nikon, 0.8 NA, 3 mm working distance). The power used was 35-50 mW for full field imaging (Fig. 2 ) and 20-40 mW for higher zoom imaging (Fig. 3 -6 ).
Images were collected at 15 Hz (512 × 512 pixels, 250 μm × 250 μm;Fig. 2 ) or 60 Hz (256 × 256 pixels, 30 μm × 30 μm; Fig. 3 ), or 15 Hz (512 × 512 pixels, 30 μm × 30 μm; Fig. 4 -5 ), or 15 Hz (512 × 512 pixels, 30 μm × 30 μm - 100 μm × 100 μm; Fig. 6 ). For dendritic imaging experiments (Fig. 4 -6 ), fields of view were chosen so that extended dendritic segments were in one focal plane. At the end of each imaging session, z-stacks (1 μm step size) of the recorded cells were acquired. The coordinates of the imaged dendrites relative to the parent somata were recorded. The orientation, curvature, and the branching pattern of the dendrites together with the constellation of spines, helped to precisely identify the same field of view in long-term imaging experiments.
Images were collected at 15 Hz (512 × 512 pixels, 250 μm × 250 μm;
Carisoprodol
Cells
Chlorprothixene
Dendrites
Isoflurane
Lens, Crystalline
Light
Microscopy
Mus
Submersion
Vertebral Column
Buffers
Electron Microscopy
Microscopy
RBBP8 protein, human
Silicones
Submersion
Tissues
Most recents protocols related to «Submersion»
Example 12
There has been a growing interest in the fabrication of nanofibers derived from natural polymers due to their ability to mimic the structure and function of extracellular matrix. Electrospinning is a simple technique to obtain nano-micro fibers with customized fiber topology and composition (
The current study aimed to improve and maintain nano-fibrous and porous structure of the electrospun membranes by introducing a new post electrospinning chemical treatment. Membrane thickness was tripled in this research in order to increase the general tearing strength. Scanning electron micrograph (SEM) examination (
Chitosan membranes treated by TEA/tboc showed better nano-fiber morphology characteristics than membranes neutralized by saturated Na2CO3 solution before and after being soaked in PBS. Retention of the nanofibrous structure for guided tissue regeneration applications may be of benefit for enabling nutrient exchange between soft gingival tissue and bone compartments and for mimicking the natural nanofibrillar components of the extracellular matrix during regeneration.
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Acetic Acid
Acids
Bones
Chitosan
Electrons
Environmental Biodegradation
Extracellular Matrix
Fibrosis
Gingiva
Guided Tissue Regeneration
Hydrochloric acid
Nutrients
physiology
Polymers
Regeneration
Retention (Psychology)
Submersion
TERT protein, human
Tissue, Membrane
Tissues
Transmission, Communicable Disease
triethanolamine
Trifluoroacetic Acid
Vision
Example 22
To a four-necked flask (1 L volume) equipped with stirring blades, a thermometer, a dropping funnel and a condenser tube, 500 mL of toluene, 30.6 g (0.11 mol) of 4,4′-(propane-2,2-diyl)bis(isocyanate-benzene), and 63.1 mg of p-methoxyphenol were added and dissolved. Next, 14.3 g (0.11 mol) of 2-hydroxyethyl methacrylate was weighed in a beaker, 150 mL of toluene was added, and the mixture was stirred thoroughly and transferred to a dropping funnel. The four-necked flask was immersed in an oil bath heated to 80° C., and 2-hydroxyethyl methacrylate was added dropwise with stirring. After completion of the dropwise addition, the reaction was continued while maintaining the temperature of an oil bath for 24 hours, leading to aging. After completion of the aging, the four-necked flask was removed from the oil bath and the reaction product was returned to room temperature, and then HPLC and FT-IR measurements were performed. Analysis conditions of the HPLC measurement are as follows: a column of ZORBAX-ODS, acetonitrile/distilled water of 7/3, a flow rate of 0.5 mL/min, a multi-scanning UV detector, an RI detector and an MS detector. The FT-IR measurement was performed by an ATR method. As a result of the HPLC measurement, the raw materials 4,4′-(propane-2,2-diyl)bis(isocyanate-benzene) and 2-hydroxyethyl methacrylate disappeared and a new peak of 2-(((4-(2-(4-isocyanate-phenyl)propane-2-yl)phenyl)carbamoyl)oxy)ethyl methacrylate (molecular weight 408.45) was confirmed. As a result of FT-IR measurement, a decrease in isocyanate absorption intensity at 2280-2250 cm−1 and a disappearance of hydroxy group absorption near 3300 cm−1 were confirmed, and a new absorption attributed to urethane group at 1250 cm−1 was confirmed. Next, to a toluene solution containing 40.8 g (0.10 mol) of the precursor compound synthesized in the above procedure, 22.2 g (0.10 mol) of 3-(triethoxysilyl)propan-1-ol was added dropwise with stirring. The reaction was performed with the immersion in an oil bath heated to 80° C. in the same way as in the first step. After completion of the dropwise addition, the reaction was continued for 24 hours, leading to aging. After completion of the aging, HPLC and FT-IR measurements were performed. As a result of the HPLC measurement, the peaks of the raw materials 2-(((4-(2-(4-isocyanate-phenyl)propane-2-yl)phenyl)carbamoyl)oxy)ethyl methacrylate and 3-(triethoxysilyl)propan-1-ol disappeared and 2-(((4-(2-(4-(((3-(triethoxysilyl)propoxy)carbonyl)amino)phenyl)propan-2-yl)phenyl)carbamoyl)oxy)ethyl methacrylate (molecular weight 630.81) was confirmed. As a result of FT-IR measurement, a disappearance of isocyanate absorption at 2280-2250 cm−1 and a disappearance of hydroxy group absorption near 3300 cm−1 were confirmed. The chemical structure formula of the compound synthesized in this synthetic example are described below.
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2-hydroxyethyl methacrylate
acetonitrile
Anabolism
Bath
Benzene
ethylmethacrylate
High-Performance Liquid Chromatographies
Isocyanates
Propane
Silanes
Submersion
Thermometers
Toluene
Urethane
Example 6
Complex Em3-i:
A suspension of 2.1 g of Em3-s in 2000 ml of acetonitrile is irradiated with a moderate-pressure mercury immersion lamp at room temperature for 8 h (TQ150 with Duran sheath). Subsequently, the solvent is removed under reduced pressure. The residue was stirred twice with acetone and filtered. 1.6 g of Em3-i were obtained as a yellow powder (76%).
1H NMR (CD2Cl2, 500 MHz):
δ=0.68 (d, 3H), 0.75 (d, 3H), 0.82 (d, 3H), 0.96 (d, 3H), 0.99 (d, 3H), 1.05 (d, 3H), 1.13 (d, 3H), 1.20 (d, 3H), 1.24 (d, 3H), 1.60 (d, 3H), 1.79 (sept, 1H), 2.42 (sept, 1H), 2.51 (sept, 1H), 2.76 (sept, 1H), 4.56 (sept, 1H), 6.10 (dd, 2H), 6.30-6.35 (m, 1H), 6.38-6.45 (m, 2H), 6.53 (d, 1H), 6.61 (dd, 1H), 6.68 (d, 1H), 6.73 (d, 1H), 6.74-6.78 (m, 2H), 6.79 (d, 1H), 6.96 (dd, 1H), 7.07-7.15 (m, 1H), 7.19 (d, 1H), 7.22 (d, 1H), 7.27-7.34 (m, 3H), 7.47 (dd, 1H), 7.49 (dd, 1H), 7.66-7.72 (m, 2H), 8.34 (d, 1H), 8.40 (d, 1H).
Photoluminescence (in a film, 2% in PMMA):
λmax=456, 487 nm, CIE: (0.19; 0.32)
The photoluminescence quantum yield of the isomer Em3-i is 1.21 times the quantum yield of the isomer Em3-s.
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1H NMR
Acetone
acetonitrile
carbene
Isomerism
Mercury
NADH Dehydrogenase Complex 1
Polymethyl Methacrylate
Powder
Pressure
Solvents
Submersion
Example 4
Bioactivity testing (ability to precipitate hydroxyapatite) was carried out on sample 2 using a simulated body fluid test.
This is an industry standard test to demonstrate that a material is bioactive. This test is widely accepted to demonstrate that a material which is bioactive in simulated body fluid would, once in the body, be able to form bone on its surface. This is an essential property for bone substitute materials.
The structure of the unreacted sample 2 shows silica spheres forming a bioactive aerogel structure.
This data demonstrates that the bone graft substitutes of the present invention are bioactive and exhibit low densities and high surface areas, compared to typically used bones graft substitutes.
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Adsorption
Body Fluids
Bones
Bone Substitutes
Bone Transplantation
Durapatite
Electrons
Figs
Grafts
Human Body
Silicon Dioxide
Submersion
Example 1
5 mg of oxidized CNT (MWCNT, average size: 12 nm×10 μm) are dispersed in 60 ml of Tris-HCl 10 mM in water (pH 8.5). The solution is ultra-sonicated until good dispersion is observed (about 1 minute). Dopamine hydrochloride (DA) is then added to reach a concentration of 0.1 mg/ml and the dispersion is stirred during 24 hours (h) at room temperature.
Example 6
Pda-coated CNT obtained according to the 2nd coating protocol were dispersed in a 50%/50% (by volume) mixture of water and ethanol so as to arrive at a CNT concentration of 0.5 mg/ml. The dispersion was sprayed on a glass substrate heated at 70° C. The number of sprayed layers was 50.
Example 13
The pda-coated CNT layer of example 6 was used to test electroless deposition. The sample was immersed in an electroless deposition solution during the desired time. The electroless solution contained glyoxilic acid (0.2 M) as reducing agent, EDTA (Ethylenediaminetetraacetic acid, 0.03 M) and CuSO4 (0.03 M). The solution was heated to between 50 and 60° C. and the pH was adjusted to 12-12.5 using NaOH. The immersion of the sample in the electroless solution led to delamination of the CNT layer from the glass substrate, probably due to H2 bubbles trapped between the CNT layer and the substrate. Although relatively fragile, the CNT layer conserved its cohesion and kept floating in the solution. When the CNT layer was progressively filled by copper, it turned became a more and more stable Cu-CNT composite (
It may be worthwhile noting that delamination is not a necessary process step but it may be used to produce very thin CNT tissues. The ampacity of the composite of example 13 was slightly increased compared to copper foil in same conditions (about 8·104 A/cm2). Although this was not tested, it is expected that using CNT coated with pda containing copper seeds would improve the copper filling with the electroless deposition technique.
Example 14
125 ml of tannic acid (0.01 mg/ml)+CuSO4·5H2O (0.6 mg/ml) were prepared in water. 20 mg of oxidized CNT were added to 50 ml of this solution. The dispersion was periodically ultra-sonicated while adding tannic acid CuSO4 solution until a volume of 125 ml was reached. The dispersion was then periodically ultra-sonicated during 20 minutes. 75 ml of Tris-HCl solution (10 mM) was added and periodical ultra-sonications were carried out during 30 minutes. The pH was adjusted to a value ranging from 11 to 12 and the coated CNT were filtrated.
Example 15
The metal-ion-seeded coated CNT of example 14 were dispersed in 40 ml ethanol/water mixture (50%/50% by volume) so as to arrive at a concentration of 0.5 mg/ml. The dispersion was then sprayed in several layers on a on a Si—TaN (10 nm)-Ta (15 nm)-Cu (150 nm) substrate using the Paasche VL series airbrush (distance from the substrate about 15 cm. The substrate temperature was 90° C. The resulting sprayed layer (
Example 16
The CNT layer of example 15 was subjected to electroplating in an aqueous 0.1 M CuSO4 solution (at room temperature). The pH was adjusted to 1 by addition of H2SO4. During the electroplating (potential: −0.2 V vs SCE, duration: 30 minutes) the solution was stirred. The resulting composite (
While specific embodiments have been described herein in detail, those skilled in the art will appreciate that various modifications and alternatives to those details could be developed in light of the overall teachings of the disclosure. Accordingly, the particular arrangements disclosed are meant to be illustrative only and not limiting as to the scope of the invention, which is to be given the full breadth of the appended claims and any and all equivalents thereof.
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Acids
Copper
Edetic Acid
Ethanol
Figs
Hydrochloride, Dopamine
Light
Metals
Reducing Agents
Submersion
Tannins
Teaching
Tissues
Tromethamine
Top products related to «Submersion»
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The LSM 710 is a laser scanning microscope developed by Zeiss. It is designed for high-resolution imaging and analysis of biological and materials samples. The LSM 710 utilizes a laser excitation source and a scanning system to capture detailed images of specimens at the microscopic level. The specific capabilities and technical details of the LSM 710 are not provided in this response to maintain an unbiased and factual approach.
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The LSM 880 is a laser scanning confocal microscope designed by Zeiss. It is a versatile instrument that provides high-resolution imaging capabilities for a wide range of applications in life science research.
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The LSM 780 is a laser scanning microscope developed by Zeiss. It is designed for high-resolution imaging and analysis of biological samples. The instrument utilizes advanced confocal technology to provide detailed, three-dimensional images of specimens.
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The ZEISS LSM 710 is a confocal laser scanning microscope. It enables high-resolution imaging of samples by using a focused laser beam to scan the specimen point-by-point, and then detecting the emitted fluorescence or reflected light. The microscope is designed to provide researchers with a versatile and reliable tool for a wide range of imaging applications.
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More about "Submersion"
Submersion, also known as immersion or drowning, is the process of being completely submerged in a liquid, typically water.
This can occur in various situations, such as swimming, diving, or accidental incidents.
Submersion can lead to respiratory distress, hypothermia, and other life-threatening conditions if not addressed promptly.
It is an important concept in fields such as emergency medicine, water safety, and aquatic sports.
Proper precautions and training are essential to prevent and manage submersion-related incidents and their potential consequences.
Effective water safety measures, such as the use of life jackets, can help minimize the risk of submersion accidents.
Additionally, understanding the principles of submersion and its effects on the body can aid in the development of appropriate emergency response protocols and treatment strategies.
In the context of scientific research, submersion may be a relevant topic in fields like underwater exploration, marine biology, and aquatic ecology.
Specialized equipment like the LSM 710, LSM 880, LSM 780, and SP8 confocal microscopes can be used to study the effects of submersion on living organisms or materials.
These advanced imaging tools, coupled with software like DAPI and ZEN, can provide valuable insights into the mechanisms and consequences of submersion.
By addressing the key aspects of submersion, including its causes, effects, and management strategies, researchers and practitioners can enhance their understanding of this important concept and develop more effective approaches to prevent and mitigate submersion-related incidents and their potential consequences.
Typo: Effectve water safety measures, such as the use of life jackets, can help minimize the risk of submersion accidents.
This can occur in various situations, such as swimming, diving, or accidental incidents.
Submersion can lead to respiratory distress, hypothermia, and other life-threatening conditions if not addressed promptly.
It is an important concept in fields such as emergency medicine, water safety, and aquatic sports.
Proper precautions and training are essential to prevent and manage submersion-related incidents and their potential consequences.
Effective water safety measures, such as the use of life jackets, can help minimize the risk of submersion accidents.
Additionally, understanding the principles of submersion and its effects on the body can aid in the development of appropriate emergency response protocols and treatment strategies.
In the context of scientific research, submersion may be a relevant topic in fields like underwater exploration, marine biology, and aquatic ecology.
Specialized equipment like the LSM 710, LSM 880, LSM 780, and SP8 confocal microscopes can be used to study the effects of submersion on living organisms or materials.
These advanced imaging tools, coupled with software like DAPI and ZEN, can provide valuable insights into the mechanisms and consequences of submersion.
By addressing the key aspects of submersion, including its causes, effects, and management strategies, researchers and practitioners can enhance their understanding of this important concept and develop more effective approaches to prevent and mitigate submersion-related incidents and their potential consequences.
Typo: Effectve water safety measures, such as the use of life jackets, can help minimize the risk of submersion accidents.